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Sequences

Sequence HMMR.eAug05

GGGAAGTGCGCTTGCGCAAATGCTAGGTTACACTTGAGTAACTCCGGGAATGACTGGGTGGGTGGGGCGGGGAACAGTAACGGTCTCCTATTGGATGGCA
GCCTAAACTCGCAGGCCAGCTAATTGGACGCAATCAGAACCCAGCATTCTTTCTTCGTGTTCCTGTGCGGGATTGGTGTGCCCAGGGGTTTGGCTTTCCA
ATTGGCTAACGCCGGGGTGGGTGGGGAATGTGGGGAGATTTGAATTTGAAACCGGTAGGGAGTGATAATCCGCATTCAGTTGTCGAGGAGTGCCAGTCAC
CTTCAGTTTCTGGAGCTGGCCGTCAACATGTCCTTTCCTAAGGCGCCCTTGAAACGATTCAATGACCCTTCTGGTTGTGCACCATCTCCAGGTGCTTATG
ATGTTAAAACTTTAGAAGTATTGAAAGGACCAGTATCCTTTCAGAAATCACAAAGATTTAAACAGCAAAAAGAATCTAAACAAAATCTTAATGTTGACAA
AGATACTACCTTGCCTGCTTCAGCTAGAAAAGTTAAGTCTTCGGAATCAAAGATTCGTGTTCTTCTACAGGAACGTGGTGCCCAGGACAGGCGGATCCAG
GATCTGGAAACTGAGTTGGAAAAGATGGAAGCAAGGCTAAATGCTGCACTAAGGGAAAAAACATCTCTCTCTGCAAATAATGCTACACTGGAAAAACAAC
TTATTGAATTGACCAGGACTAATGAACTACTAAAATCTAAGGTATCTGAGCCTCATGATAATATTTACAATTGAATAAATATAAACACGTTTTTTAGGGC
CGGGCACGGTGGCTCACGCCTGTGTTCCCAGCATTTTGGGAGGCCAAGGCAGGCGGATCACCTGAGGTCGGGAGTTCGAGACCAGCCTGACCAACATGGA
GAAACCCTGTCTCTACTAAAAATACAAAAATTAGCTAGGCCTATTGGCGGGCGCCTGTAATCTCAGCTACTCGGGAGGCTGAGGCAGAAGAATCACTTGA
ACCCAGGAGGTGGAGGTTGCAGTGAGCTGACATGGCGCCATTGCACTTCAGCCTAGGCAAGAGAGTGCGACTCCATCTCAAAAAACAAAACAAAACAAAA
CAACATACACCAGGCGATTCAGATTCATTTTGTTAAGCAAACATGTTTCAAGAAATCCTTTCTCAGGGACTTGCTTTAGAGTCTTGCAATGGACTTTCCA
GCATATAGAGGTAAGTGACCACCCAAACAACCTTTCAAAAATGAACCAACAGGCCAGCCACAGTGGCTCACACCTGTAATTCCAGCGCTTTGGGAAGCTG
AGACGGATGGATCACTTGAGGTCAGGATTTTGAGACTAGCCTAGCCTAACATGGCGAAACCCCATCTTTACTAAAAATACAAAAATTAGTCAGGTATGGT
GGCGTACACCCGTAATTCCAGCTACTCAGGAGGCTGAGGCATGAGAATAGCTTGAACTCGGGAGGCTGAGGCATGAGAATAGCTTGAACTCGGGAGGTGG
AAGTTGCAGTGAGCAACACGGCACTCCAGCCTGGGTGACAGAGTGAGACTCTGTCTTACAAAAATAAAATAAAAATAAACCAGCAAGTCACATTAAGGAA
AAGAGGGATAAGAACAGTAGACTGGTACAGTGGCTCATGCCTGTATTTCCAGCATTTTGGAAGGCTGAGGGCTGGAGAATTGCTTGAGGCCAGGAGTTTG
AGACCAGCCTGGGCAACATATCAAGACCCCATCTCTATAAACAAATTGAAAAATTAGCTAGGCATGGTGGTGGTGCACACCGGTAATCCCAGCTACTCAG
GAAGATGAGGCAGGAGGATTGATTGAGCCCAGGAGTTTGAGATTATAGCGAGCTATGATCATGCCACTCCACTCTAGCCTGGACAGCGGAGCGAGACTTG
ATCTCTTAAAAAG
  • HMMR.F1 (forward primer)
  • HMMR.R1 (reverse primer)
  • Single exon skipping

Frame 0

GKCACANARLHLSNSGNDWVGGAGNSNGLLLDG
SLNSQAS*LDAIRTQHSFFVFLCGIGVPRGLAFQ
LANAGVGGECGEI*I*NR*GVIIRIQLSRSASH
LQFLELAVNMSFPKAPLKRFNDPSGCAPSPGAY
DVKTLEVLKGPVSFQKSQRFKQQKESKQNLNVDK
DTTLPASARKVKSSESKIRVLLQERGAQDRRIQ
DLETELEKMEARLNAALREKTSLSANNATLEKQ
LIELTRTNELLKSKVSEPHDNIYN*ININTFFRA
GHGGSRLCSQHFGRPRQADHLRSGVRDQPDQHG
ETLSLLKIQKLARPIGGRL*SQLLGRLRQKNHL
NPGGGGCSELTWRHCTSA*ARECDSISKNKTKQN
NIHQAIQIHFVKQTCFKKSFLRDLL*SLAMDFP
AYRGK*PPKQPFKNEPTGQPQWLTPVIPALWEA
ETDGSLEVRILRLA*PNMAKPHLY*KYKN*SGMV
AYTRNSSYSGG*GMRIA*TREAEA*E*LELGRW
KLQ*ATRHSSLGDRVRLCLTKIK*K*TSKSH*G
KEG*EQ*TGTVAHACISSILEG*GLENCLRPGV*
DQPGQHIKTPSL*TN*KIS*AWWWCTPVIPATQ
EDEAGGLIEPRSLRL*RAMIMPLHSSLDSGARL
DLLK

Frame 1

GSALAQMLGYT*VTPGMTGWVGRGTVTVSYWMA
A*TRRPANWTQSEPSILSSCSCAGLVCPGVWLS
NWLTPGWVGNVGRFEFETGRE**SAFSCRGVPVT
FSFWSWPSTCPFLRRP*NDSMTLLVVHHLQVLM
MLKL*KY*KDQYPFRNHKDLNSKKNLNKILMLT
KILPCLLQLEKLSLRNQRFVFFYRNVVPRTGGSR
IWKLSWKRWKQG*MLH*GKKHLSLQIMLHWKNN
LLN*PGLMNY*NLRYLSLMIIFTIE*I*TRFLG
PGTVAHACVPSILGGQGRRIT*GREFETSLTNME
KPCLY*KYKN*LGLLAGACNLSYSGG*GRRIT*
TQEVEVAVS*HGAIALQPRQESATPSQKTKQNK
TTYTRRFRFILLSKHVSRNPFSGTCFRVLQWTFQ
HIEVSDHPNNLSKMNQQASHSGSHL*FQRFGKL
RRMDHLRSGF*D*PSLTWRNPIFTKNTKISQVW
WRTPVIPATQEAEA*E*LELGRLRHENSLNSGGG
SCSEQHGTPAWVTE*DSVLQK*NKNKPASHIKE
KRDKNSRLVQWLMPVFPAFWKAEGWRIA*GQEF
ETSLGNISRPHLYKQIEKLARHGGGAHR*SQLLR
KMRQED*LSPGV*DYSEL*SCHSTLAWTAERDL
IS*K

Frame 2

EVRLRKC*VTLE*LRE*LGGWGGEQ*RSPIGWQ
PKLAGQLIGRNQNPAFFLRVPVRDWCAQGFGFP
IG*RRGGWGMWGDLNLKPVGSDNPHSVVEECQS
PSVSGAGRQHVLS*GALETIQ*PFWLCTISRCL*
C*NFRSIERTSILSEITKI*TAKRI*TKS*C*Q
RYYLACFS*KS*VFGIKDSCSSTGTWCPGQADP
GSGN*VGKDGSKAKCCTKGKNISLCK*CYTGKTT
Y*IDQD**TTKI*GI*AS**YLQLNKYKHVF*G
RARWLTPVFPAFWEAKAGGSPEVGSSRPA*PTW
RNPVSTKNTKIS*AYWRAPVISATREAEAEESLE
PRRWRLQ*ADMAPLHFSLGKRVRLHLKKQNKTK
QHTPGDSDSFC*ANMFQEILSQGLALESCNGLS
SI*R*VTTQTTFQK*TNRPATVAHTCNSSALGS*
DGWIT*GQDFETSLA*HGETPSLLKIQKLVRYG
GVHP*FQLLRRLRHENSLNSGG*GMRIA*TREV
EVAVSNTALQPG*QSETLSYKNKIKINQQVTLRK
RGIRTVDWYSGSCLYFQHFGRLRAGELLEARSL
RPAWATYQDPISINKLKN*LGMVVVHTGNPSYS
GR*GRRID*AQEFEIIASYDHATPL*PGQRSET*
SLKK

Sequence HMMR.cAug05

GGGAAGTGCGCTTGCGCAAATGCTAGGTTACACTTGAGTAACTCCGGGAATGACTGGGTGGGTGGGGCGGGGAACAGTAACGGTCTCCTATTGGATGGCA
GCCTAAACTCGCAGGCCAGCTAATTGGACGCAATCAGAACCCAGCATTCTTTCTTCGTGTTCCTGTGCGGGATTGGTGTGCCCAGGGGTTTGGCTTTCCA
ATTGGCTAACGCCGGGGTGGGTGGGGAATGTGGGGAGATTTGAATTTGAAACCGGTAGGGAGTGATAATCCGCATTCAGTTGTCGAGGAGTGCCAGTCAC
CTTCAGTTTCTGGAGCTGGCCGTCAACATGTCCTTTCCTAAGGCGCCCTTGAAACGATTCAATGACCCTTCTGGTTGTGCACCATCTCCAGGTGCTTATG
ATGTTAAAACTTTAGAAGTATTGAAAGGACCAGTATCCTTTCAGAAATCACAAAGATTTAAACAGCAAAAAGAATCTAAACAAAATCTTAATGTTGACAA
AGATACTACCTTGCCTGCTTCAGCTAGAAAAGTTAAGTCTTCGGAATCAAAGATTCGTGTTCTTCTACAGGAACGTGGTGCCCAGGACAGGCGGATCCAG
GATCTGGAAACTGAGTTGGAAAAGATGGAAGCAAGGCTAAATGCTGCACTAAGGGAAAAAACATCTCTCTCTGCAAATAATGCTACACTGGAAAAACAAC
TTATTGAATTGACCAGGACTAATGAACTACTAAAATCTAAGTTTTCTGAAAATGGTAACCAGAAGAATTTGAGAATTCTAAGCTTGGAGTTGATGAAACT
TAGAAACAAAAGAGAAACAAAGATGAGGGGTATGATGGCTAAGCAAGAAGGCATGGAGATGAAGCTGCAGGTCACCCAAAGGAGTCTCGAAGAGTCTCAA
GGGAAAATAGCCCAACTGGAGGGAAAACTTGTTTCAATAGAGAAAGAAAAGATTGATGAAAAATCTGAAACAGAAAAACTCTTGGAATACATCGAAGAAA
TTAGTTGTGCTTCAGATCAAGTGGAAAAATACAAGCTAGATATTGCCCAGTTAGAAGAAAATTTGAAAGAGAAGAATGATGAAATTTTAAGCCTTAAGCA
GTCTCTTGAGGAGAATATTGTTATATTATCTAAACAAGTAGAAGATCTAAATGTGAAATGTCAGCTGCTTGAAAAAGAAAAAGAAGACCATGTCAACAGG
AATAGAGAACACAACGAAAATCTAAATGCAGAGATGCAAAACTTAAAACAGAAGTTTATTCTTGAACAACAGGAACGTGAAAAGCTTCAACAAAAAGAAT
TACAAATTGATTCACTTCTGCAACAAGAGAAAGAATTATCTTCGAGTCTTCATCAGAAGCTCTGTTCTTTTCAAGAGGAAATGGTTAAAGAGAAGAATCT
GTTTGAGGAAGAATTAAAGCAAACACTGGATGAGCTTGATAAATTACAGCAAAAGGAGGAACAAGCTGAAAGGCTGGTCAAGCAATTGGAAGAGGAAGCA
AAATCTAGAGCTGAAGAATTAAAACTCCTAGAAGAAAAGCTGAAAGGGAAGGAGGCTGAACTGGAGAAAAGTAGTGCTGCTCATACCCAGGCCACCCTGC
TTTTGCAGGAAAAGTATGACAGTATGGTGCAAAGCCTTGAAGATGTTACTGCTCAATTTGAAAGCTATAAAGCGTTAACAGCCAGTGAGATAGAAGATCT
TAAGCTGGAGAACTCATCATTACAGGAAAAAGCGGCCAAGGCTGGGAAAAATGCAGAGGATGTTCAGCATCAGATTTTGGCAACTGAGAGCTCAAATCAA
GAATATGTAAGGATGCTTCTAGATCTGCAGACCAAGTCAGCACTAAAGGAAACAGAAATTAAAGAAATCACAGTTTCTTTTCTTCAAAAAATAACTGATT
TGCAGAACCAACTCAAGCAACAGGAGGAAGACTTTAGAAAACAGCTGGAAGATGAAGAAGGAAGAAAAGCTGAAAAAGAAAATACAACAGCAGAATTAAC
TGAAGAAATTAACAAGTGGCGTCTCCTCTATGAAGAACTATATAATAAAACAAAACCTTTTCAGCTACAACTAGATGCTTTTGAAGTAGAAAAACAGGCA
TTGTTGAATGAACATGGTGCAGCTCAGGAACAGCTAAATAAAATAAGAGATTCATATGCTAAATTATTGGGTCATCAGAATTTGAAACAAAAAATCAAGC
ATGTTGTGAAGTTGAAAGATGAAAATAGCCAACTCAAATCGGAAGTATCAAAACTCCGCTGTCAGCTTGCTAAAAAAAAACAAAGTGAGACAAAACTTCA
AGAGGAATTGAATAAAGTTCTAGGTATCAAACACTTTGATCCTTCAAAGGCTTTTCATCATGAAAGTAAAGAAAATTTTGCCCTGAAGACCCCATTAAAA
GAAGGCAATACAAACTGTTACCGAGCTCCTATGGAGTGTCAAGAATCATGGAAGTAAACATCTGAGAAACCTGTTGAAGATTATTTCATTCGTCTTGTTG
TTATTGATGTTGCTGTTATTATATTTGACATGGGTATTTTATAATGTTGTATTTAATTTTAACTGCCAATCCTTAAATATGTGAAAGGAACATTTTTTAC
CAAAGTGTCTTTTGACATTTTATTTTTTCTTGCAAATACCTCCTCCCTAATGCTCACCTTTATCACCTCATTCTGAACCCTTTCGCTGGCTTTCCAGCTT
AGAATGCATCTCATCAACTTAAAAGTCAGTATCATATTATTATCCTCCTGTTCTGAAACCTTAGTTTCAAGAGTCTAAACCCCAGATTCTTCAGCTTGAT
CCTGGAGGTCTTTTCTAGTCTGAGCTTCTTTAGCTAGGCTAAAACACCTTGGCTTGTTATTGCCTCTACTTTGATTCTGATAATGCTCACTTGGTCCTAC
CTATTATCCTTCTACTTGTCCAGTTCAAATAAGAAATAAGGACAAGCCTAACTTCATAGAAACCTCTCTATTTTTAATCAGTTGTTTAATAATTTACAGG
TTCTTAGGCTCCATCCTGTTTGTATGAAATTATAATCTGTGGATTGGCCTTTAAGCCTGCATTCTTAACAAACTCTTCAGTTAATTCTTAGATACACTAA
AAATCTGAGAAACTCTACATGTAACTATTTCTTCAGAGTTTGTCATATACTGCTTGTCATCTGCATGTCTACTCAGCATTTGATTAACATTTGTGTAATA
TGAAATAAAATTACACAGTAAGTCATTTAACCAATTAA
  • HMMR.F1 (forward primer)
  • HMMR.R1 (reverse primer)
  • Single exon skipping

Frame 0

GKCACANARLHLSNSGNDWVGGAGNSNGLLLDG
SLNSQAS*LDAIRTQHSFFVFLCGIGVPRGLAFQ
LANAGVGGECGEI*I*NR*GVIIRIQLSRSASH
LQFLELAVNMSFPKAPLKRFNDPSGCAPSPGAY
DVKTLEVLKGPVSFQKSQRFKQQKESKQNLNVDK
DTTLPASARKVKSSESKIRVLLQERGAQDRRIQ
DLETELEKMEARLNAALREKTSLSANNATLEKQ
LIELTRTNELLKSKFSENGNQKNLRILSLELMKL
RNKRETKMRGMMAKQEGMEMKLQVTQRSLEESQ
GKIAQLEGKLVSIEKEKIDEKSETEKLLEYIEE
ISCASDQVEKYKLDIAQLEENLKEKNDEILSLKQ
SLEENIVILSKQVEDLNVKCQLLEKEKEDHVNR
NREHNENLNAEMQNLKQKFILEQQEREKLQQKE
LQIDSLLQQEKELSSSLHQKLCSFQEEMVKEKNL
FEEELKQTLDELDKLQQKEEQAERLVKQLEEEA
KSRAEELKLLEEKLKGKEAELEKSSAAHTQATL
LLQEKYDSMVQSLEDVTAQFESYKALTASEIEDL
KLENSSLQEKAAKAGKNAEDVQHQILATESSNQ
EYVRMLLDLQTKSALKETEIKEITVSFLQKITD
LQNQLKQQEEDFRKQLEDEEGRKAEKENTTAELT
EEINKWRLLYEELYNKTKPFQLQLDAFEVEKQA
LLNEHGAAQEQLNKIRDSYAKLLGHQNLKQKIK
HVVKLKDENSQLKSEVSKLRCQLAKKKQSETKLQ
EELNKVLGIKHFDPSKAFHHESKENFALKTPLK
EGNTNCYRAPMECQESWK*TSEKPVEDYFIRLV
VIDVAVIIFDMGIL*CCI*F*LPILKYVKGTFFT
KVSFDILFFLANTSSLMLTFITSF*TLSLAFQL
RMHLINLKVSIILLSSCSETLVSRV*TPDSSA*
SWRSFLV*ASLARLKHLGLLLPLL*F**CSLGPT
YYPSTCPVQIRNKDKPNFIETSLFLISCLIIYR
FLGSILFV*NYNLWIGL*ACILNKLFS*FLDTL
KI*ETLHVTISSEFVIYCLSSACLLSI*LTFV*Y
EIKLHSKSFNQL

Frame 1

GSALAQMLGYT*VTPGMTGWVGRGTVTVSYWMA
A*TRRPANWTQSEPSILSSCSCAGLVCPGVWLS
NWLTPGWVGNVGRFEFETGRE**SAFSCRGVPVT
FSFWSWPSTCPFLRRP*NDSMTLLVVHHLQVLM
MLKL*KY*KDQYPFRNHKDLNSKKNLNKILMLT
KILPCLLQLEKLSLRNQRFVFFYRNVVPRTGGSR
IWKLSWKRWKQG*MLH*GKKHLSLQIMLHWKNN
LLN*PGLMNY*NLSFLKMVTRRI*EF*AWS**N
LETKEKQR*GV*WLSKKAWR*SCRSPKGVSKSLK
GK*PNWRENLFQ*RKKRLMKNLKQKNSWNTSKK
LVVLQIKWKNTS*ILPS*KKI*KRRMMKF*ALS
SLLRRILLYYLNK*KI*M*NVSCLKKKKKTMSTG
IENTTKI*MQRCKT*NRSLFLNNRNVKSFNKKN
YKLIHFCNKRKNYLRVFIRSSVLFKRKWLKRRI
CLRKN*SKHWMSLINYSKRRNKLKGWSSNWKRKQ
NLELKN*NS*KKS*KGRRLNWRKVVLLIPRPPC
FCRKSMTVWCKALKMLLLNLKAIKR*QPVR*KI
LSWRTHHYRKKRPRLGKMQRMFSIRFWQLRAQIK
NM*GCF*ICRPSQH*RKQKLKKSQFLFFKK*LI
CRTNSSNRRKTLENSWKMKKEEKLKKKIQQQN*
LKKLTSGVSSMKNYIIKQNLFSYN*MLLK*KNRH
C*MNMVQLRNS*IK*EIHMLNYWVIRI*NKKSS
ML*S*KMKIANSNRKYQNSAVSLLKKNKVRQNF
KRN*IKF*VSNTLILQRLFIMKVKKILP*RPH*K
KAIQTVTELLWSVKNHGSKHLRNLLKIISFVLL
LLMLLLLYLTWVFYNVVFNFNCQSLNM*KEHFL
PKCLLTFYFFLQIPPP*CSPLSPHSEPFRWLSSL
ECISST*KSVSYYYPPVLKP*FQESKPQILQLD
PGGLF*SELL*LG*NTLACYCLYFDSDNAHLVL
PIILLLVQFK*EIRTSLTS*KPLYF*SVV**FTG
S*APSCLYEIIICGLAFKPAFLTNSSVNS*IH*
KSEKLYM*LFLQSLSYTACHLHVYSAFD*HLCN
MK*NYTVSHLTN*

Frame 2

EVRLRKC*VTLE*LRE*LGGWGGEQ*RSPIGWQ
PKLAGQLIGRNQNPAFFLRVPVRDWCAQGFGFP
IG*RRGGWGMWGDLNLKPVGSDNPHSVVEECQS
PSVSGAGRQHVLS*GALETIQ*PFWLCTISRCL*
C*NFRSIERTSILSEITKI*TAKRI*TKS*C*Q
RYYLACFS*KS*VFGIKDSCSSTGTWCPGQADP
GSGN*VGKDGSKAKCCTKGKNISLCK*CYTGKTT
Y*IDQD**TTKI*VF*KW*PEEFENSKLGVDET
*KQKRNKDEGYDG*ARRHGDEAAGHPKESRRVS
RENSPTGGKTCFNRERKD**KI*NRKTLGIHRRN
*LCFRSSGKIQARYCPVRRKFEREE**NFKP*A
VS*GEYCYII*TSRRSKCEMSAA*KRKRRPCQQ
E*RTQRKSKCRDAKLKTEVYS*TTGT*KASTKRI
TN*FTSATRERIIFESSSEALFFSRGNG*REES
V*GRIKANTG*A**ITAKGGTS*KAGQAIGRGS
KI*S*RIKTPRRKAEREGG*TGEK*CCSYPGHPA
FAGKV*QYGAKP*RCYCSI*KL*SVNSQ*DRRS
*AGELIITGKSGQGWEKCRGCSASDFGN*ELKS
RICKDASRSADQVSTKGNRN*RNHSFFSSKNN*F
AEPTQATGGRL*KTAGR*RRKKS*KRKYNSRIN
*RN*QVASPL*RTI**NKTFSATTRCF*SRKTG
IVE*TWCSSGTAK*NKRFIC*IIGSSEFETKNQA
CCEVER*K*PTQIGSIKTPLSAC*KKTK*DKTS
RGIE*SSRYQTL*SFKGFSS*K*RKFCPEDPIK
RRQYKLLPSSYGVSRIMEVNI*ETC*RLFHSSCC
Y*CCCYYI*HGYFIMLYLILTANP*ICERNIFY
QSVF*HFIFSCKYLLPNAHLYHLILNPFAGFPA
*NASHQLKSQYHIIILLF*NLSFKSLNPRFFSLI
LEVFSSLSFFS*AKTPWLVIASTLILIMLTWSY
LLSFYLSSSNKK*GQA*LHRNLSIFNQLFNNLQ
VLRLHPVCMKL*SVDWPLSLHS*QTLQLILRYTK
NLRNSTCNYFFRVCHILLVICMSTQHLINICVI
*NKITQ*VI*PI

Sequence HMMR.aAug05

GGGAAGTGCGCTTGCGCAAATGCTAGGTTACACTTGAGTAACTCCGGGAATGACTGGGTGGGTGGGGCGGGGAACAGTAACGGTCTCCTATTGGATGGCA
GCCTAAACTCGCAGGCCAGCTAATTGGACGCAATCAGAACCCAGCATTCTTTCTTCGTGTTCCTGTGCGGGATTGGTGTGCCCAGGGGTTTGGCTTTCCA
ATTGGCTAACGCCGGGGTGGGTGGGGAATGTGGGGAGATTTGAATTTGAAACCGGTAGGGAGTGATAATCCGCATTCAGTTGTCGAGGAGTGCCAGTCAC
CTTCAGTTTCTGGAGCTGGCCGTCAACATGTCCTTTCCTAAGGCGCCCTTGAAACGATTCAATGACCCTTCTGGTTGTGCACCATCTCCAGGTGCTTATG
ATGTTAAAACTTTAGAAGTATTGAAAGGACCAGTATCCTTTCAGAAATCACAAAGATTTAAACAGCAAAAAGAATCTAAACAAAATCTTAATGTTGACAA
AGATACTACCTTGCCTGCTTCAGCTAGAAAAGTTAAGTCTTCGGAATCAAAGAAGGAATCTCAAAAGAATGATAAAGATTTGAAGATATTAGAGAAAGAG
ATTCGTGTTCTTCTACAGGAACGTGGTGCCCAGGACAGGCGGATCCAGGATCTGGAAACTGAGTTGGAAAAGATGGAAGCAAGGCTAAATGCTGCACTAA
GGGAAAAAACATCTCTCTCTGCAAATAATGCTACACTGGAAAAACAACTTATTGAATTGACCAGGACTAATGAACTACTAAAATCTAAGTTTTCTGAAAA
TGGTAACCAGAAGAATTTGAGAATTCTAAGCTTGGAGTTGATGAAACTTAGAAACAAAAGAGAAACAAAGATGAGGGGTATGATGGCTAAGCAAGAAGGC
ATGGAGATGAAGCTGCAGGTCACCCAAAGGAGTCTCGAAGAGTCTCAAGGGAAAATAGCCCAACTGGAGGGAAAACTTGTTTCAATAGAGAAAGAAAAGA
TTGATGAAAAATCTGAAACAGAAAAACTCTTGGAATACATCGAAGAAATTAGTTGTGCTTCAGATCAAGTGGAAAAATACAAGCTAGATATTGCCCAGTT
AGAAGAAAATTTGAAAGAGAAGAATGATGAAATTTTAAGCCTTAAGCAGTCTCTTGAGGAGAATATTGTTATATTATCTAAACAAGTAGAAGATCTAAAT
GTGAAATGTCAGCTGCTTGAAAAAGAAAAAGAAGACCATGTCAACAGGAATAGAGAACACAACGAAAATCTAAATGCAGAGATGCAAAACTTAAAACAGA
AGTTTATTCTTGAACAACAGGAACGTGAAAAGCTTCAACAAAAAGAATTACAAATTGATTCACTTCTGCAACAAGAGAAAGAATTATCTTCGAGTCTTCA
TCAGAAGCTCTGTTCTTTTCAAGAGGAAATGGTTAAAGAGAAGAATCTGTTTGAGGAAGAATTAAAGCAAACACTGGATGAGCTTGATAAATTACAGCAA
AAGGAGGAACAAGCTGAAAGGCTGGTCAAGCAATTGGAAGAGGAAGCAAAATCTAGAGCTGAAGAATTAAAACTCCTAGAAGAAAAGCTGAAAGGGAAGG
AGGCTGAACTGGAGAAAAGTAGTGCTGCTCATACCCAGGCCACCCTGCTTTTGCAGGAAAAGTATGACAGTATGGTGCAAAGCCTTGAAGATGTTACTGC
TCAATTTGAAAGCTATAAAGCGTTAACAGCCAGTGAGATAGAAGATCTTAAGCTGGAGAACTCATCATTACAGGAAAAAGCGGCCAAGGCTGGGAAAAAT
GCAGAGGATGTTCAGCATCAGATTTTGGCAACTGAGAGCTCAAATCAAGAATATGTAAGGATGCTTCTAGATCTGCAGACCAAGTCAGCACTAAAGGAAA
CAGAAATTAAAGAAATCACAGTTTCTTTTCTTCAAAAAATAACTGATTTGCAGAACCAACTCAAGCAACAGGAGGAAGACTTTAGAAAACAGCTGGAAGA
TGAAGAAGGAAGAAAAGCTGAAAAAGAAAATACAACAGCAGAATTAACTGAAGAAATTAACAAGTGGCGTCTCCTCTATGAAGAACTATATAATAAAACA
AAACCTTTTCAGCTACAACTAGATGCTTTTGAAGTAGAAAAACAGGCATTGTTGAATGAACATGGTGCAGCTCAGGAACAGCTAAATAAAATAAGAGATT
CATATGCTAAATTATTGGGTCATCAGAATTTGAAACAAAAAATCAAGCATGTTGTGAAGTTGAAAGATGAAAATAGCCAACTCAAATCGGAAGTATCAAA
ACTCCGCTGTCAGCTTGCTAAAAAAAAACAAAGTGAGACAAAACTTCAAGAGGAATTGAATAAAGTTCTAGGTATCAAACACTTTGATCCTTCAAAGGCT
TTTCATCATGAAAGTAAAGAAAATTTTGCCCTGAAGACCCCATTAAAAGAAGGCAATACAAACTGTTACCGAGCTCCTATGGAGTGTCAAGAATCATGGA
AGTAAACATCTGAGAAACCTGTTGAAGATTATTTCATTCGTCTTGTTGTTATTGATGTTGCTGTTATTATATTTGACATGGGTATTTTATAATGTTGTAT
TTAATTTTAACTGCCAATCCTTAAATATGTGAAAGGAACATTTTTTACCAAAGTGTCTTTTGACATTTTATTTTTTCTTGCAAATACCTCCTCCCTAATG
CTCACCTTTATCACCTCATTCTGAACCCTTTCGCTGGCTTTCCAGCTTAGAATGCATCTCATCAACTTAAAAGTCAGTATCATATTATTATCCTCCTGTT
CTGAAACCTTAGTTTCAAGAGTCTAAACCCCAGATTCTTCAGCTTGATCCTGGAGGTCTTTTCTAGTCTGAGCTTCTTTAGCTAGGCTAAAACACCTTGG
CTTGTTATTGCCTCTACTTTGATTCTGATAATGCTCACTTGGTCCTACCTATTATCCTTCTACTTGTCCAGTTCAAATAAGAAATAAGGACAAGCCTAAC
TTCATAGAAACCTCTCTATTTTTAATCAGTTGTTTAATAATTTACAGGTTCTTAGGCTCCATCCTGTTTGTATGAAATTATAATCTGTGGATTGGCCTTT
AAGCCTGCATTCTTAACAAACTCTTCAGTTAATTCTTAGATACACTAAAAATCTGAGAAACTCTACATGTAACTATTTCTTCAGAGTTTGTCATATACTG
CTTGTCATCTGCATGTCTACTCAGCATTTGATTAACATTTGTGTAATATGAAATAAAATTACACAGTAAGTCATTTAACCAATTAA
  • HMMR.F1 (forward primer)
  • HMMR.R1 (reverse primer)
  • Single exon skipping

Frame 0

GKCACANARLHLSNSGNDWVGGAGNSNGLLLDG
SLNSQAS*LDAIRTQHSFFVFLCGIGVPRGLAFQ
LANAGVGGECGEI*I*NR*GVIIRIQLSRSASH
LQFLELAVNMSFPKAPLKRFNDPSGCAPSPGAY
DVKTLEVLKGPVSFQKSQRFKQQKESKQNLNVDK
DTTLPASARKVKSSESKKESQKNDKDLKILEKE
IRVLLQERGAQDRRIQDLETELEKMEARLNAAL
REKTSLSANNATLEKQLIELTRTNELLKSKFSEN
GNQKNLRILSLELMKLRNKRETKMRGMMAKQEG
MEMKLQVTQRSLEESQGKIAQLEGKLVSIEKEK
IDEKSETEKLLEYIEEISCASDQVEKYKLDIAQL
EENLKEKNDEILSLKQSLEENIVILSKQVEDLN
VKCQLLEKEKEDHVNRNREHNENLNAEMQNLKQ
KFILEQQEREKLQQKELQIDSLLQQEKELSSSLH
QKLCSFQEEMVKEKNLFEEELKQTLDELDKLQQ
KEEQAERLVKQLEEEAKSRAEELKLLEEKLKGK
EAELEKSSAAHTQATLLLQEKYDSMVQSLEDVTA
QFESYKALTASEIEDLKLENSSLQEKAAKAGKN
AEDVQHQILATESSNQEYVRMLLDLQTKSALKE
TEIKEITVSFLQKITDLQNQLKQQEEDFRKQLED
EEGRKAEKENTTAELTEEINKWRLLYEELYNKT
KPFQLQLDAFEVEKQALLNEHGAAQEQLNKIRD
SYAKLLGHQNLKQKIKHVVKLKDENSQLKSEVSK
LRCQLAKKKQSETKLQEELNKVLGIKHFDPSKA
FHHESKENFALKTPLKEGNTNCYRAPMECQESW
K*TSEKPVEDYFIRLVVIDVAVIIFDMGIL*CCI
*F*LPILKYVKGTFFTKVSFDILFFLANTSSLM
LTFITSF*TLSLAFQLRMHLINLKVSIILLSSC
SETLVSRV*TPDSSA*SWRSFLV*ASLARLKHLG
LLLPLL*F**CSLGPTYYPSTCPVQIRNKDKPN
FIETSLFLISCLIIYRFLGSILFV*NYNLWIGL
*ACILNKLFS*FLDTLKI*ETLHVTISSEFVIYC
LSSACLLSI*LTFV*YEIKLHSKSFNQL

Frame 1

GSALAQMLGYT*VTPGMTGWVGRGTVTVSYWMA
A*TRRPANWTQSEPSILSSCSCAGLVCPGVWLS
NWLTPGWVGNVGRFEFETGRE**SAFSCRGVPVT
FSFWSWPSTCPFLRRP*NDSMTLLVVHHLQVLM
MLKL*KY*KDQYPFRNHKDLNSKKNLNKILMLT
KILPCLLQLEKLSLRNQRRNLKRMIKI*RY*RKR
FVFFYRNVVPRTGGSRIWKLSWKRWKQG*MLH*
GKKHLSLQIMLHWKNNLLN*PGLMNY*NLSFLK
MVTRRI*EF*AWS**NLETKEKQR*GV*WLSKKA
WR*SCRSPKGVSKSLKGK*PNWRENLFQ*RKKR
LMKNLKQKNSWNTSKKLVVLQIKWKNTS*ILPS
*KKI*KRRMMKF*ALSSLLRRILLYYLNK*KI*M
*NVSCLKKKKKTMSTGIENTTKI*MQRCKT*NR
SLFLNNRNVKSFNKKNYKLIHFCNKRKNYLRVF
IRSSVLFKRKWLKRRICLRKN*SKHWMSLINYSK
RRNKLKGWSSNWKRKQNLELKN*NS*KKS*KGR
RLNWRKVVLLIPRPPCFCRKSMTVWCKALKMLL
LNLKAIKR*QPVR*KILSWRTHHYRKKRPRLGKM
QRMFSIRFWQLRAQIKNM*GCF*ICRPSQH*RK
QKLKKSQFLFFKK*LICRTNSSNRRKTLENSWK
MKKEEKLKKKIQQQN*LKKLTSGVSSMKNYIIKQ
NLFSYN*MLLK*KNRHC*MNMVQLRNS*IK*EI
HMLNYWVIRI*NKKSSML*S*KMKIANSNRKYQ
NSAVSLLKKNKVRQNFKRN*IKF*VSNTLILQRL
FIMKVKKILP*RPH*KKAIQTVTELLWSVKNHG
SKHLRNLLKIISFVLLLLMLLLLYLTWVFYNVV
FNFNCQSLNM*KEHFLPKCLLTFYFFLQIPPP*C
SPLSPHSEPFRWLSSLECISST*KSVSYYYPPV
LKP*FQESKPQILQLDPGGLF*SELL*LG*NTL
ACYCLYFDSDNAHLVLPIILLLVQFK*EIRTSLT
S*KPLYF*SVV**FTGS*APSCLYEIIICGLAF
KPAFLTNSSVNS*IH*KSEKLYM*LFLQSLSYT
ACHLHVYSAFD*HLCNMK*NYTVSHLTN*

Frame 2

EVRLRKC*VTLE*LRE*LGGWGGEQ*RSPIGWQ
PKLAGQLIGRNQNPAFFLRVPVRDWCAQGFGFP
IG*RRGGWGMWGDLNLKPVGSDNPHSVVEECQS
PSVSGAGRQHVLS*GALETIQ*PFWLCTISRCL*
C*NFRSIERTSILSEITKI*TAKRI*TKS*C*Q
RYYLACFS*KS*VFGIKEGISKE**RFEDIRER
DSCSSTGTWCPGQADPGSGN*VGKDGSKAKCCTK
GKNISLCK*CYTGKTTY*IDQD**TTKI*VF*K
W*PEEFENSKLGVDET*KQKRNKDEGYDG*ARR
HGDEAAGHPKESRRVSRENSPTGGKTCFNRERKD
**KI*NRKTLGIHRRN*LCFRSSGKIQARYCPV
RRKFEREE**NFKP*AVS*GEYCYII*TSRRSK
CEMSAA*KRKRRPCQQE*RTQRKSKCRDAKLKTE
VYS*TTGT*KASTKRITN*FTSATRERIIFESS
SEALFFSRGNG*REESV*GRIKANTG*A**ITA
KGGTS*KAGQAIGRGSKI*S*RIKTPRRKAEREG
G*TGEK*CCSYPGHPAFAGKV*QYGAKP*RCYC
SI*KL*SVNSQ*DRRS*AGELIITGKSGQGWEK
CRGCSASDFGN*ELKSRICKDASRSADQVSTKGN
RN*RNHSFFSSKNN*FAEPTQATGGRL*KTAGR
*RRKKS*KRKYNSRIN*RN*QVASPL*RTI**N
KTFSATTRCF*SRKTGIVE*TWCSSGTAK*NKRF
IC*IIGSSEFETKNQACCEVER*K*PTQIGSIK
TPLSAC*KKTK*DKTSRGIE*SSRYQTL*SFKG
FSS*K*RKFCPEDPIKRRQYKLLPSSYGVSRIME
VNI*ETC*RLFHSSCCY*CCCYYI*HGYFIMLY
LILTANP*ICERNIFYQSVF*HFIFSCKYLLPN
AHLYHLILNPFAGFPA*NASHQLKSQYHIIILLF
*NLSFKSLNPRFFSLILEVFSSLSFFS*AKTPW
LVIASTLILIMLTWSYLLSFYLSSSNKK*GQA*
LHRNLSIFNQLFNNLQVLRLHPVCMKL*SVDWPL
SLHS*QTLQLILRYTKNLRNSTCNYFFRVCHIL
LVICMSTQHLINICVI*NKITQ*VI*PI

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