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Sequence NM_014145
ACTTCCGGCGCACTGATCCCGCCTGGGGCCGGCTGAGTGGCACTTAAGCGGGCCATGCCATGCAACCTTGGGCGCTGCCAACCGTGGGCGAGCTCTGGGTGTGCGGGCGGCCTGGCGCGGCGCTCCGGTAAGGCGTGTGTGCGGCAGGGCGGGGACAGAACCGTCCTCTCGGGCTCTGGGCGTGTCCGAGACCGCGCTCC
CCGCCGAAATCAAGCTCCGAGTCATCCGTGTGGGGCATTCGTCCCCCCTGGCACAGTTGGCCTCTTTCCAGAAGCCCGTTTTGTTTGTTTTACGTCTAAA
TTCGCGTCGGTTCTTATTTCTCTCCCTGGCAAGGTCTGAAGACGGGTAGGAGAATAACCTGTGTCAGCGTGTTATGATGCCGTCCCGTACCAACCTGGCT
ACTGGAATCCCCAGTAGTAAAGTGAAATATTCAAGGCTCTCCAGCACAGACGATGGCTACATTGACCTTCAGTTTAAGAAAACCCCTCCTAAGATCCCTT
ATAAGGCCATCGCACTTGCCACTGTGCTGTTTTTGATTGGCGCCTTTCTCATTATTATAGGCTCCCTCCTGCTGTCAGGCTACATCAGCAAAGGGGGGGC
AGACCGGGCCGTTCCAGTGCTGATCATTGGCATTCTGGTGTTCCTACCCGGATTTTACCACCTGCGCATCGCTTACTATGCATCCAAAGGCTACCGTGGT
TACTCCTATGATGACATTCCAGACTTTGATGACTAGCACCCACCCCATAGCTGAGGAGGAGTCACAGTGGAACTGTCCCAGCTTTAAGATATCTAGCAGA
AACTATAGCTGAGGACTAAGGAATTCTGCAGCTTGCAGATGTTTAAGAAAATAATGGCCAGATTTTTTGGGTCCTTCCCAAAGATGTTAAGTGAACCTAC
AGTTAGCTAATTAGGACAAGCTCTATTTTTCATCCCTGGGCCCTGACAAGTTTTTCCACAGGAATATGTATCATGGAAGAATAGAGGTTATTCTGTAATG
GAAAAGTGTTGCCTGCCACCACCCTCTGTAGAGCTGAGCATTTCTTTTAAATAGTCTTCATTGCCAATTTGTTCTTGTAGCAAATGGAACAATGTGGTAT
GGCTAATTTCTTATTATTAAGTAATTTATTTTAAAAATATCTGAGTATATTATCCTGTACACTTATCCCTACCTTCATGTTCCAGTGGAAGACCTTAGTA
AAATCAAAGATCAGTGAGTTCATCTGTAATATTTTTTTTACTTGCTTTCTTACTGACAGCAACCAGGAATTTTTTTATCCTGCAGAGCAAGTTTTCAAAA
TGTAAATACTTCCTCTGTTTAACAGTCCTTGGACCATTCTGATCCAGTTCACCAGTAGGTTGGACAGCATATAATTTGCATCATTTTGTCCCTTGTAAAT
CAAGATGTTCTGCAGATTATTCCTTTAACGGCCGGACTTTTGGCTGTTTCCTAATGAAACATGTAGTGGTTATTATTTAGAGTTTATAGCCGTATTGCTA
GCACCTTGTAGTATGTCATCATTCTGCTCATGATTCCAAGGATCAGCCTGGATGCCTAGAGGACTAGATCACCTTAGTTTGATTCTATTTTTTAGCTTGC
AAAAAGTGACTTATATTCCAAAGAAATTAAAATGTTGAAATCCAAATCCTAGAAATAAAATGAGTTAACTTCAAACATTTCAGAAAACA
- refseq_C20orf30.F2 (forward primer)
- refseq_C20orf30.R2 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
TSGALIPPGAG*VALKRAMPCNLGRCQPWASSGCAGGLARRSGKACVRQGGDRTVLSGSGRVRDRAP
RRNQAPSHPCGAFVPPGTVGLFPEARFVCFTSK
FASVLISLPGKV*RRVGE*PVSACYDAVPYQPG
YWNPQ**SEIFKALQHRRWLH*PSV*ENPS*DPL
*GHRTCHCAVFDWRLSHYYRLPPAVRLHQQRGG
RPGRSSADHWHSGVPTRILPPAHRLLCIQRLPW
LLL**HSRL**LAPTP*LRRSHSGTVPALRYLAE
TIAED*GILQLADV*ENNGQIFWVLPKDVK*TY
S*LIRTSSIFHPWALTSFSTGICIMEE*RLFCN
GKVLPATTLCRAEHFF*IVFIANLFL*QMEQCGM
ANFLLLSNLF*KYLSILSCTLIPTFMFQWKTLV
KSKISEFICNIFFTCFLTDSNQEFFYPAEQVFK
M*ILPLFNSPWTILIQFTSRLDSI*FASFCPL*I
KMFCRLFL*RPDFWLFPNETCSGYYLEFIAVLL
APCSMSSFCS*FQGSAWMPRGLDHLSLILFFSL
QKVTYIPKKLKC*NPNPRNKMS*LQTFQKT
Frame 1
LPAH*SRLGPAEWHLSGPCHATLGAANRGRALGVRAAWRGAPVRRVCGRAGTEPSSRALGVSETAL
PAEIKLRVIRVGHSSPLAQLASFQKPVLFVLRLN
SRRFLFLSLARSEDG*ENNLCQRVMMPSRTNLA
TGIPSSKVKYSRLSSTDDGYIDLQFKKTPPKIP
YKAIALATVLFLIGAFLIIIGSLLLSGYISKGGA
DRAVPVLIIGILVFLPGFYHLRIAYYASKGYRG
YSYDDIPDFDD*HPPHS*GGVTVELSQL*DI*Q
KL*LRTKEFCSLQMFKKIMARFFGSFPKMLSEPT
VS*LGQALFFIPGP*QVFPQEYVSWKNRGYSVM
EKCCLPPPSVELSISFK*SSLPICSCSKWNNVV
WLISYY*VIYFKNI*VYYPVHLSLPSCSSGRP**
NQRSVSSSVIFFLLAFLLTATRNFFILQSKFSK
CKYFLCLTVLGPF*SSSPVGWTAYNLHHFVPCK
SRCSADYSFNGRTFGCFLMKHVVVII*SL*PYC*
HLVVCHHSAHDSKDQPGCLED*ITLV*FYFLAC
KK*LIFQRN*NVEIQILEIK*VNFKHFRK
Frame 2
FRRTDPAWGRLSGT*AGHAMQPWALPTVGELWVCGRPGAALR*GVCAAGRGQNRPLGLWACPRPRS
PPKSSSESSVWGIRPPWHSWPLSRSPFCLFYV*
IRVGSYFSPWQGLKTGRRITCVSVL*CRPVPTWL
LESPVVK*NIQGSPAQTMATLTFSLRKPLLRSL
IRPSHLPLCCF*LAPFSLL*APSCCQATSAKGG
QTGPFQC*SLAFWCSYPDFTTCASLTMHPKATVV
TPMMTFQTLMTSTHPIAEEESQWNCPSFKISSR
NYS*GLRNSAACRCLRK*WPDFLGPSQRC*VNL
QLAN*DKLYFSSLGPDKFFHRNMYHGRIEVIL*W
KSVACHHPL*S*AFLLNSLHCQFVLVANGTMWY
G*FLIIK*FILKISEYIILYTYPYLHVPVEDLS
KIKDQ*VHL*YFFYLLSY*QQPGIFLSCRASFQN
VNTSSV*QSLDHSDPVHQ*VGQHIICIILSLVN
QDVLQIIPLTAGLLAVS**NM*WLLFRVYSRIA
STL*YVIILLMIPRISLDA*RTRSP*FDSIF*LA
KSDLYSKEIKMLKSKS*K*NELTSNISEN
Sequence NM_001009924
ACTTCCGGCGCACTGATCCCGCCTGGGGCCGGCTGAGTGGCACTTAAGCGGGCCATGCCATGCAACCTTGGGCGCTGCCAACCGTGGGCGAGCTCTGGGTGTGCGGGCGGCCTGGCGCGGCGCTCCGGTAAGGCGTGTGTGCGGCAGGGCGGGGACAGAACCGTCCTCTCGGGCTCTGGGCGTGTCCGAGACCGCGCTCC
CCGCCGAAATCAAGCTCCGAGTCATCCGTGTGGGGCATTCGTCCCCCCTGGCACAGTTGGCCTCTTTCCAGAAGCCCGTTTTGTTTGTTTTACGTCTAAA
TTCGCGTCGGTTCTTATTTCTCTCCCTGGCAAGGTCTGAAGACGGATGGAGTCTAGTTCTGTCGCCCAGGCTGGAGCCCAGTGGTGTGATCTCAGCTCAC
TGCAATCTCCACCTCCTGGCTTCAAGCGATTCTTCTGCTTCAGCCTCCCGACTGTGTCAGCGTGTTATGATGCCGTCCCGTACCAACCTGGCTACTGGAA
TCCCCAGTAGTAAAGTGAAATATTCAAGGCTCTCCAGCACAGACGATGGCTACATTGACCTTCAGTTTAAGAAAACCCCTCCTAAGATCCCTTATAAGGC
CATCGCACTTGCCACTGTGCTGTTTTTGATTGGCGCCTTTCTCATTATTATAGGCTCCCTCCTGCTGTCAGGCTACATCAGCAAAGGGGGGGCAGACCGG
GCCGTTCCAGTGCTGATCATTGGCATTCTGGTGTTCCTACCCGGATTTTACCACCTGCGCATCGCTTACTATGCATCCAAAGGCTACCGTGGTTACTCCT
ATGATGACATTCCAGACTTTGATGACTAGCACCCACCCCATAGCTGAGGAGGAGTCACAGTGGAACTGTCCCAGCTTTAAGATATCTAGCAGAAACTATA
GCTGAGGACTAAGGAATTCTGCAGCTTGCAGATGTTTAAGAAAATAATGGCCAGATTTTTTGGGTCCTTCCCAAAGATGTTAAGTGAACCTACAGTTAGC
TAATTAGGACAAGCTCTATTTTTCATCCCTGGGCCCTGACAAGTTTTTCCACAGGAATATGTATCATGGAAGAATAGAGGTTATTCTGTAATGGAAAAGT
GTTGCCTGCCACCACCCTCTGTAGAGCTGAGCATTTCTTTTAAATAGTCTTCATTGCCAATTTGTTCTTGTAGCAAATGGAACAATGTGGTATGGCTAAT
TTCTTATTATTAAGTAATTTATTTTAAAAATATCTGAGTATATTATCCTGTACACTTATCCCTACCTTCATGTTCCAGTGGAAGACCTTAGTAAAATCAA
AGATCAGTGAGTTCATCTGTAATATTTTTTTTACTTGCTTTCTTACTGACAGCAACCAGGAATTTTTTTATCCTGCAGAGCAAGTTTTCAAAATGTAAAT
ACTTCCTCTGTTTAACAGTCCTTGGACCATTCTGATCCAGTTCACCAGTAGGTTGGACAGCATATAATTTGCATCATTTTGTCCCTTGTAAATCAAGATG
TTCTGCAGATTATTCCTTTAACGGCCGGACTTTTGGCTGTTTCCTAATGAAACATGTAGTGGTTATTATTTAGAGTTTATAGCCGTATTGCTAGCACCTT
GTAGTATGTCATCATTCTGCTCATGATTCCAAGGATCAGCCTGGATGCCTAGAGGACTAGATCACCTTAGTTTGATTCTATTTTTTAGCTTGCAAAAAGT
GACTTATATTCCAAAGAAATTAAAATGTTGAAATCCAAATCCTAGAAATAAAATGAGTTAACTTCAAACATTTCAGAAAACA
- refseq_C20orf30.F2 (forward primer)
- refseq_C20orf30.R2 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
TSGALIPPGAG*VALKRAMPCNLGRCQPWASSGCAGGLARRSGKACVRQGGDRTVLSGSGRVRDRAP
RRNQAPSHPCGAFVPPGTVGLFPEARFVCFTSK
FASVLISLPGKV*RRMESSSVAQAGAQWCDLSS
LQSPPPGFKRFFCFSLPTVSACYDAVPYQPGYWN
PQ**SEIFKALQHRRWLH*PSV*ENPS*DPL*G
HRTCHCAVFDWRLSHYYRLPPAVRLHQQRGGRP
GRSSADHWHSGVPTRILPPAHRLLCIQRLPWLLL
**HSRL**LAPTP*LRRSHSGTVPALRYLAETI
AED*GILQLADV*ENNGQIFWVLPKDVK*TYS*
LIRTSSIFHPWALTSFSTGICIMEE*RLFCNGKV
LPATTLCRAEHFF*IVFIANLFL*QMEQCGMAN
FLLLSNLF*KYLSILSCTLIPTFMFQWKTLVKS
KISEFICNIFFTCFLTDSNQEFFYPAEQVFKM*I
LPLFNSPWTILIQFTSRLDSI*FASFCPL*IKM
FCRLFL*RPDFWLFPNETCSGYYLEFIAVLLAP
CSMSSFCS*FQGSAWMPRGLDHLSLILFFSLQKV
TYIPKKLKC*NPNPRNKMS*LQTFQKT
Frame 1
LPAH*SRLGPAEWHLSGPCHATLGAANRGRALGVRAAWRGAPVRRVCGRAGTEPSSRALGVSETAL
PAEIKLRVIRVGHSSPLAQLASFQKPVLFVLRLN
SRRFLFLSLARSEDGWSLVLSPRLEPSGVISAH
CNLHLLASSDSSASASRLCQRVMMPSRTNLATG
IPSSKVKYSRLSSTDDGYIDLQFKKTPPKIPYKA
IALATVLFLIGAFLIIIGSLLLSGYISKGGADR
AVPVLIIGILVFLPGFYHLRIAYYASKGYRGYS
YDDIPDFDD*HPPHS*GGVTVELSQL*DI*QKL*
LRTKEFCSLQMFKKIMARFFGSFPKMLSEPTVS
*LGQALFFIPGP*QVFPQEYVSWKNRGYSVMEK
CCLPPPSVELSISFK*SSLPICSCSKWNNVVWLI
SYY*VIYFKNI*VYYPVHLSLPSCSSGRP**NQ
RSVSSSVIFFLLAFLLTATRNFFILQSKFSKCK
YFLCLTVLGPF*SSSPVGWTAYNLHHFVPCKSRC
SADYSFNGRTFGCFLMKHVVVII*SL*PYC*HL
VVCHHSAHDSKDQPGCLED*ITLV*FYFLACKK
*LIFQRN*NVEIQILEIK*VNFKHFRK
Frame 2
FRRTDPAWGRLSGT*AGHAMQPWALPTVGELWVCGRPGAALR*GVCAAGRGQNRPLGLWACPRPRS
PPKSSSESSVWGIRPPWHSWPLSRSPFCLFYV*
IRVGSYFSPWQGLKTDGV*FCRPGWSPVV*SQLT
AISTSWLQAILLLQPPDCVSVL*CRPVPTWLLE
SPVVK*NIQGSPAQTMATLTFSLRKPLLRSLIR
PSHLPLCCF*LAPFSLL*APSCCQATSAKGGQTG
PFQC*SLAFWCSYPDFTTCASLTMHPKATVVTP
MMTFQTLMTSTHPIAEEESQWNCPSFKISSRNY
S*GLRNSAACRCLRK*WPDFLGPSQRC*VNLQLA
N*DKLYFSSLGPDKFFHRNMYHGRIEVIL*WKS
VACHHPL*S*AFLLNSLHCQFVLVANGTMWYG*
FLIIK*FILKISEYIILYTYPYLHVPVEDLSKIK
DQ*VHL*YFFYLLSY*QQPGIFLSCRASFQNVN
TSSV*QSLDHSDPVHQ*VGQHIICIILSLVNQD
VLQIIPLTAGLLAVS**NM*WLLFRVYSRIASTL
*YVIILLMIPRISLDA*RTRSP*FDSIF*LAKS
DLYSKEIKMLKSKS*K*NELTSNISEN
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