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Sequences

Sequence NM_033340

GAAAGATCCAGGAGCAGCATCTCCACCAGCAAGCTGGGCTGCTGGGTGGGTACTTCCTTCAAAGCTGAGGGAGCGTCCTACGCCCACGCGCGCAGGAGGG CGCCCCCCGCAAAGCAACGTCTAGGAGACCACAGTGGATGCCACAGCGGGCCCGAAGCGGATCAGCCTTGTGGGATGGCAGATGATCAGGGCTGTATTGA AGAGCAGGGGGTTGAGGATTCAGCAAATGAAGATTCAGTGGATGCTAAGCCAGACCGGTCCTCGTTTGTACCGTCCCTCTTCAGTAAGAAGAAGAAAAAT GTCACCATGCGATCCATCAAGACCACCCGGGACCGAGTGCCTACATATCAGTACAACATGAATTTTGAAAAGCTGGGCAAATGCATCATAATAAACAACA AGAACTTTGATAAAGTGACAGGTATGGGCGTTCGAAACGGAACAGACAAAGATGCCGAGGCGCTCTTCAAGTGCTTCCGAAGCCTGGGTTTTGACGTGAT TGTCTATAATGACTGCTCTTGTGCCAAGATGCAAGATCTGCTTAAAAAAGCTTCTGAAGAGGACCATACAAATGCCGCCTGCTTCGCCTGCATCCTCTTA AGCCATGGAGAAGAAAATATGGAATCTTGCTCTGTCACCCAGGCTGGAGTGCAGCGGCGTGATCTCGGAAGACTGCAACCTCCACCTCCCAGGCTTGCCG AGGGACCGAGCTTGATGATGGCATCCAGGCCGACTCGGGGCCCATCAATGACACAGATGCTAATCCTCGATACAAGATCCCAGTGGAAGCTGACTTCCTC TTCGCCTATTCCACGGTTCCAGGCTATTACTCGTGGAGGAGCCCAGGAAGAGGCTCCTGGTTTGTGCAAGCCCTCTGCTCCATCCTGGAGGAGCACGGAA AAGACCTGGAAATCATGCAGATCCTCACCAGGGTGAATGACAGAGTTGCCAGGCACTTTGAGTCTCAGTCTGATGACCCACACTTCCATGAGAAGAAGCA GATCCCCTGTGTGGTCTCCATGCTCACCAAGGAACTCTACTTCAGTCAATAGCCATATCAGGGGTACATTCTAGCTGAGAAGCAATGGGTCACTCATTAA TGAATCACATTTTTTTATGCTCTTGAAATATTCAGAAATTCTCCAGGATTTTAATTTCAGGAAAATGTATTGATTCAACAGGGAAGAAACTTTCTGGTGC TGTCTTTTGTTCTCTGAATTTTCAGAGACTTTTTTTATAATGTTATTCATTTGGTGACTGTGTAACTTTCTCTTAAGATTAATTTTCTCTTTGTATGTCT GTTACCTTGTTAATAGACTTAATACATGCAACAGAAGTGACTTCTGGAGAAAGCTCATGGCTGTGTCCACTGCAATTGGTGGTAACAGTGGTAGAGTCAT GTTTGCACTTGGCAAAAAGAATCCCAATGTTTGACAAAACACAGCCAAGGGGATATTTACTGCTCTTTATTGCAGAATGTGGGTATTGAGTGTGATTTGA ATGATTTTTCATTGGCTTAGGGCAGATTTTCATGCAAAAGTTCTCATATGAGTTAGAGGAGAAAAAGCTTAATGATTCTGATATGTATCCATCAGGATCC AGTCTGGAAAACAGAAACCATTCTAGGTGTTTCAACAGAGGGAGTTTAATACAGGAAATTGACTTACATAGATGATAAAAGAGAAGCCAAACAGCAAGAA GCTGTTACCACACCCAGGGCTATGAGGATAATGGGAAGAGGTTTGGTTTCCTGTGTCCAGTAGTGGGATCATCCAGAGGAGCTGGAACCATGGTGGGGGC TGCCTAGTGGGAGTTAGGACCACCAATGGATTGTGGAAAATGGAGCCATGACAAGAACAAAGCCACTGACTGAGATGGAGTGAGCTGAGACAGATAAGAG AATACCTTGGTCTCACCTATCCTGCCCTCACATCTTCCACCAGCACCTTACTGCCCAGGCCTATCTGGAAGCCACCTCACCAAGGACCTTGGAAGAGCAA GGGACAGTGAGGCAGGAGAAGAACAAGAAATGGATGTAAGCCTGGCCCATAATGTGAACATAAGTAATCACTAATGCTCAACAATTTATCCATTCAATCA TTTATTCATTGGGTTGTCAGATAGTCTATGTATGTGTAAAACAATCTGTTTTGGCTTTATGTGCAAAATCTGTTATAGCTTTAAAATATATCTGGAACTT TTTAGATTATTCCAAGCCTTATTTTGAGTAAATATTTGTTACTTTTAGTTCTATAAGTGAGGAAGAGTTTATGGCAAAGATTTTTGGCACTTTGTTTTCA AGATGGTGTTATCTTTTGAATTCTTGATAAATGACTGTTTTTTTCTGCCTAATAGTAACTGGTTAAAAAACAAATGTTCATATTTATTGATTAAAAATGT GGTTGCTTAATTCCTAA

refseq_CASP7.F1 (forward primer)
refseq_CASP7.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

ERSRSSISTSKLGCWVGTSFKAEGASYAHARRR APPAKQRLGDHSGCHSGPEADQPCGMADDQGCIE EQGVEDSANEDSVDAKPDRSSFVPSLFSKKKKN VTMRSIKTTRDRVPTYQYNMNFEKLGKCIIINN KNFDKVTGMGVRNGTDKDAEALFKCFRSLGFDVI VYNDCSCAKMQDLLKKASEEDHTNAACFACILL SHGEENMESCSVTQAGVQRRDLGRLQPPPPRLA EGPSLMMASRPTRGPSMTQMLILDTRSQWKLTSS SPIPRFQAITRGGAQEEAPGLCKPSAPSWRSTE KTWKSCRSSPG*MTELPGTLSLSLMTHTSMRRS RSPVWSPCSPRNSTSVNSHIRGTF*LRSNGSLIN ESHFFMLLKYSEILQDFNFRKMY*FNREETFWC CLLFSEFSETFFIMLFIW*LCNFLLRLIFSLYV CYLVNRLNTCNRSDFWRKLMAVSTAIGGNSGRVM FALGKKNPNV*QNTAKGIFTALYCRMWVLSVI* MIFHWLRADFHAKVLI*VRGEKA**F*YVSIRI QSGKQKPF*VFQQREFNTGN*LT*MIKEKPNSKK LLPHPGL*G*WEEVWFPVSSSGIIQRSWNHGGG CLVGVRTTNGLWKMEP*QEQSH*LRWSELRQIR EYLGLTYPALTSSTSTLLPRPIWKPPHQGPWKSK GQ*GRRRTRNGCKPGP*CEHK*SLMLNNLSIQS FIHWVVR*SMYV*NNLFWLYVQNLL*L*NISGT F*IIPSLILSKYLLLLVL*VRKSLWQRFLALCFQ DGVIF*ILDK*LFFSA***LVKKQMFIFID*KC GCLIP

Frame 1

KDPGAASPPASWAAGWVLPSKLRERPTPTRAGG RPPQSNV*ETTVDATAGPKRISLVGWQMIRAVL KSRGLRIQQMKIQWMLSQTGPRLYRPSSVRRRKM SPCDPSRPPGTECLHISTT*ILKSWANAS**TT RTLIK*QVWAFETEQTKMPRRSSSASEAWVLT* LSIMTALVPRCKICLKKLLKRTIQMPPASPASS* AMEKKIWNLALSPRLECSGVISEDCNLHLPGLP RDRA**WHPGRLGAHQ*HRC*SSIQDPSGS*LP LRLFHGSRLLLVEEPRKRLLVCASPLLHPGGARK RPGNHADPHQGE*QSCQAL*VSV**PTLP*EEA DPLCGLHAHQGTLLQSIAISGVHSS*EAMGHSL MNHIFLCS*NIQKFSRILISGKCIDSTGKKLSGA VFCSLNFQRLFL*CYSFGDCVTFS*D*FSLCMS VTLLIDLIHATEVTSGESSWLCPLQLVVTVVES CLHLAKRIPMFDKTQPRGYLLLFIAECGY*V*FE *FFIGLGQIFMQKFSYELEEKKLNDSDMYPSGS SLENRNHSRCFNRGSLIQEIDLHR**KRSQTAR SCYHTQGYEDNGKRFGFLCPVVGSSRGAGTMVGA A*WELGPPMDCGKWSHDKNKATD*DGVS*DR*E NTLVSPILPSHLPPAPYCPGLSGSHLTKDLGRA RDSEAGEEQEMDVSLAHNVNISNH*CSTIYPFNH LFIGLSDSLCMCKTICFGFMCKICYSFKIYLEL FRLFQALF*VNICYF*FYK*GRVYGKDFWHFVF KMVLSFEFLINDCFFLPNSNWLKNKCSYLLIKNV VA*FL

Frame 2

KIQEQHLHQQAGLLGGYFLQS*GSVLRPRAQEG APRKATSRRPQWMPQRARSGSALWDGR*SGLY* RAGG*GFSK*RFSGC*ARPVLVCTVPLQ*EEEK CHHAIHQDHPGPSAYISVQHEF*KAGQMHHNKQQ EL**SDRYGRSKRNRQRCRGALQVLPKPGF*RD CL**LLLCQDARSA*KSF*RGPYKCRLLRLHPL KPWRRKYGILLCHPGWSAAA*SRKTATSTSQACR GTELDDGIQADSGPINDTDANPRYKIPVEADFL FAYSTVPGYYSWRSPGRGSWFVQALCSILEEHG KDLEIMQILTRVNDRVARHFESQSDDPHFHEKKQ IPCVVSMLTKELYFSQ*PYQGYILAEKQWVTH* *ITFFYALEIFRNSPGF*FQENVLIQQGRNFLV LSFVL*IFRDFFYNVIHLVTV*LSLKINFLFVCL LPC**T*YMQQK*LLEKAHGCVHCNWW*QW*SH VCTWQKESQCLTKHSQGDIYCSLLQNVGIECDL NDFSLA*GRFSCKSSHMS*RRKSLMILICIHQDP VWKTETILGVSTEGV*YRKLTYIDDKREAKQQE AVTTPRAMRIMGRGLVSCVQ*WDHPEELEPWWG LPSGS*DHQWIVENGAMTRTKPLTEME*AETDKR IPWSHLSCPHIFHQHLTAQAYLEATSPRTLEEQ GTVRQEKNKKWM*AWPIM*T*VITNAQQFIHSI IYSLGCQIVYVCVKQSVLALCAKSVIALKYIWNF LDYSKPYFE*IFVTFSSISEEEFMAKIFGTLFS RWCYLLNS**MTVFFCLIVTG*KTNVHIY*LKM WLLNS*

Sequence NM_033339

CTCCCGCGCGCGGGCTCAACTTTGTAGAGCGAGGGGCCAACTTGGCAGAGCGCGCGGCCAGCTTTGCAGAGAGCGCCCTCCAGGGACTATGCGTGCGGGG ACACGGGTCGCTTTGGGCTCTTCCACCCCTGCGGAGCGCACTACCCCGAGCCAGGGGCGGTGCAAGCCCCGCCCGGCCCTACCCAGGGCGGCTCCTCCCT CCGCAGCGCCGAGACTTTTAGTTTCGCTTTCGCTAAAGGGGCCCCAGACCCTTGCTGCGGAGCGACGGAGAGAGACTGTGCCAGTCCCAGCCGCCCTACC GCCGTGGGAACGGCAGGAAGTGGCACTTGGAAAAGAACACCAGCTGCGGTGGTAGCAGTGGGATTTGTGCTTCTTATGTTACCCAGATGGCAGATGATCA GGGCTGTATTGAAGAGCAGGGGGTTGAGGATTCAGCAAATGAAGATTCAGTGGATGCTAAGCCAGACCGGTCCTCGTTTGTACCGTCCCTCTTCAGTAAG AAGAAGAAAAATGTCACCATGCGATCCATCAAGACCACCCGGGACCGAGTGCCTACATATCAGTACAACATGAATTTTGAAAAGCTGGGCAAATGCATCA TAATAAACAACAAGAACTTTGATAAAGTGACAGGTATGGGCGTTCGAAACGGAACAGACAAAGATGCCGAGGCGCTCTTCAAGTGCTTCCGAAGCCTGGG TTTTGACGTGATTGTCTATAATGACTGCTCTTGTGCCAAGATGCAAGATCTGCTTAAAAAAGCTTCTGAAGAGGACCATACAAATGCCGCCTGCTTCGCC TGCATCCTCTTAAGCCATGGAGAAGAAAATGTAATTTATGGGAAAGATGGTGTCACACCAATAAAGGATTTGACAGCCCACTTTAGGGGGGATAGATGCA AAACCCTTTTAGAGAAACCCAAACTCTTCTTCATTCAGGCTTGCCGAGGGACCGAGCTTGATGATGGCATCCAGGCCGACTCGGGGCCCATCAATGACAC AGATGCTAATCCTCGATACAAGATCCCAGTGGAAGCTGACTTCCTCTTCGCCTATTCCACGGTTCCAGGCTATTACTCGTGGAGGAGCCCAGGAAGAGGC TCCTGGTTTGTGCAAGCCCTCTGCTCCATCCTGGAGGAGCACGGAAAAGACCTGGAAATCATGCAGATCCTCACCAGGGTGAATGACAGAGTTGCCAGGC ACTTTGAGTCTCAGTCTGATGACCCACACTTCCATGAGAAGAAGCAGATCCCCTGTGTGGTCTCCATGCTCACCAAGGAACTCTACTTCAGTCAATAGCC ATATCAGGGGTACATTCTAGCTGAGAAGCAATGGGTCACTCATTAATGAATCACATTTTTTTATGCTCTTGAAATATTCAGAAATTCTCCAGGATTTTAA TTTCAGGAAAATGTATTGATTCAACAGGGAAGAAACTTTCTGGTGCTGTCTTTTGTTCTCTGAATTTTCAGAGACTTTTTTTATAATGTTATTCATTTGG TGACTGTGTAACTTTCTCTTAAGATTAATTTTCTCTTTGTATGTCTGTTACCTTGTTAATAGACTTAATACATGCAACAGAAGTGACTTCTGGAGAAAGC TCATGGCTGTGTCCACTGCAATTGGTGGTAACAGTGGTAGAGTCATGTTTGCACTTGGCAAAAAGAATCCCAATGTTTGACAAAACACAGCCAAGGGGAT ATTTACTGCTCTTTATTGCAGAATGTGGGTATTGAGTGTGATTTGAATGATTTTTCATTGGCTTAGGGCAGATTTTCATGCAAAAGTTCTCATATGAGTT AGAGGAGAAAAAGCTTAATGATTCTGATATGTATCCATCAGGATCCAGTCTGGAAAACAGAAACCATTCTAGGTGTTTCAACAGAGGGAGTTTAATACAG GAAATTGACTTACATAGATGATAAAAGAGAAGCCAAACAGCAAGAAGCTGTTACCACACCCAGGGCTATGAGGATAATGGGAAGAGGTTTGGTTTCCTGT GTCCAGTAGTGGGATCATCCAGAGGAGCTGGAACCATGGTGGGGGCTGCCTAGTGGGAGTTAGGACCACCAATGGATTGTGGAAAATGGAGCCATGACAA GAACAAAGCCACTGACTGAGATGGAGTGAGCTGAGACAGATAAGAGAATACCTTGGTCTCACCTATCCTGCCCTCACATCTTCCACCAGCACCTTACTGC CCAGGCCTATCTGGAAGCCACCTCACCAAGGACCTTGGAAGAGCAAGGGACAGTGAGGCAGGAGAAGAACAAGAAATGGATGTAAGCCTGGCCCATAATG TGAACATAAGTAATCACTAATGCTCAACAATTTATCCATTCAATCATTTATTCATTGGGTTGTCAGATAGTCTATGTATGTGTAAAACAATCTGTTTTGG CTTTATGTGCAAAATCTGTTATAGCTTTAAAATATATCTGGAACTTTTTAGATTATTCCAAGCCTTATTTTGAGTAAATATTTGTTACTTTTAGTTCTAT AAGTGAGGAAGAGTTTATGGCAAAGATTTTTGGCACTTTGTTTTCAAGATGGTGTTATCTTTTGAATTCTTGATAAATGACTGTTTTTTTCTGCCTAATA GTAACTGGTTAAAAAACAAATGTTCATATTTATTGATTAAAAATGTGGTTGCTTAATTCCTAA

refseq_CASP7.F1 (forward primer)
refseq_CASP7.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

LPRAGSTL*SEGPTWQSARPALQRAPSRDYACG DTGRFGLFHPCGAHYPEPGAVQAPPGPTQGGSSL RSAETFSFAFAKGAPDPCCGATERDCASPSRPT AVGTAGSGTWKRTPAAVVAVGFVLLMLPRWQMI RAVLKSRGLRIQQMKIQWMLSQTGPRLYRPSSVR RRKMSPCDPSRPPGTECLHISTT*ILKSWANAS **TTRTLIK*QVWAFETEQTKMPRRSSSASEAW VLT*LSIMTALVPRCKICLKKLLKRTIQMPPASP ASS*AMEKKM*FMGKMVSHQ*RI*QPTLGGIDA KPF*RNPNSSSFRLAEGPSLMMASRPTRGPSMT QMLILDTRSQWKLTSSSPIPRFQAITRGGAQEEA PGLCKPSAPSWRSTEKTWKSCRSSPG*MTELPG TLSLSLMTHTSMRRSRSPVWSPCSPRNSTSVNS HIRGTF*LRSNGSLINESHFFMLLKYSEILQDFN FRKMY*FNREETFWCCLLFSEFSETFFIMLFIW *LCNFLLRLIFSLYVCYLVNRLNTCNRSDFWRK LMAVSTAIGGNSGRVMFALGKKNPNV*QNTAKGI FTALYCRMWVLSVI*MIFHWLRADFHAKVLI*V RGEKA**F*YVSIRIQSGKQKPF*VFQQREFNT GN*LT*MIKEKPNSKKLLPHPGL*G*WEEVWFPV SSSGIIQRSWNHGGGCLVGVRTTNGLWKMEP*Q EQSH*LRWSELRQIREYLGLTYPALTSSTSTLL PRPIWKPPHQGPWKSKGQ*GRRRTRNGCKPGP*C EHK*SLMLNNLSIQSFIHWVVR*SMYV*NNLFW LYVQNLL*L*NISGTF*IIPSLILSKYLLLLVL *VRKSLWQRFLALCFQDGVIF*ILDK*LFFSA** *LVKKQMFIFID*KCGCLIP

Frame 1

SRARAQLCRARGQLGRARGQLCRERPPGTMRAG TRVALGSSTPAERTTPSQGRCKPRPALPRAAPP SAAPRLLVSLSLKGPQTLAAERRRETVPVPAALP PWERQEVALGKEHQLRW*QWDLCFLCYPDGR*S GLY*RAGG*GFSK*RFSGC*ARPVLVCTVPLQ* EEEKCHHAIHQDHPGPSAYISVQHEF*KAGQMHH NKQQEL**SDRYGRSKRNRQRCRGALQVLPKPG F*RDCL**LLLCQDARSA*KSF*RGPYKCRLLR LHPLKPWRRKCNLWERWCHTNKGFDSPL*GG*MQ NPFRETQTLLHSGLPRDRA**WHPGRLGAHQ*H RC*SSIQDPSGS*LPLRLFHGSRLLLVEEPRKR LLVCASPLLHPGGARKRPGNHADPHQGE*QSCQA L*VSV**PTLP*EEADPLCGLHAHQGTLLQSIA ISGVHSS*EAMGHSLMNHIFLCS*NIQKFSRIL ISGKCIDSTGKKLSGAVFCSLNFQRLFL*CYSFG DCVTFS*D*FSLCMSVTLLIDLIHATEVTSGES SWLCPLQLVVTVVESCLHLAKRIPMFDKTQPRG YLLLFIAECGY*V*FE*FFIGLGQIFMQKFSYEL EEKKLNDSDMYPSGSSLENRNHSRCFNRGSLIQ EIDLHR**KRSQTARSCYHTQGYEDNGKRFGFL CPVVGSSRGAGTMVGAA*WELGPPMDCGKWSHDK NKATD*DGVS*DR*ENTLVSPILPSHLPPAPYC PGLSGSHLTKDLGRARDSEAGEEQEMDVSLAHN VNISNH*CSTIYPFNHLFIGLSDSLCMCKTICFG FMCKICYSFKIYLELFRLFQALF*VNICYF*FY K*GRVYGKDFWHFVFKMVLSFEFLINDCFFLPN SNWLKNKCSYLLIKNVVA*FL

Frame 2

PARGLNFVERGANLAERAASFAESALQGLCVRG HGSLWALPPLRSALPRARGGASPARPYPGRLLP PQRRDF*FRFR*RGPRPLLRSDGERLCQSQPPY RRGNGRKWHLEKNTSCGGSSGICASYVTQMADDQ GCIEEQGVEDSANEDSVDAKPDRSSFVPSLFSK KKKNVTMRSIKTTRDRVPTYQYNMNFEKLGKCI IINNKNFDKVTGMGVRNGTDKDAEALFKCFRSLG FDVIVYNDCSCAKMQDLLKKASEEDHTNAACFA CILLSHGEENVIYGKDGVTPIKDLTAHFRGDRC KTLLEKPKLFFIQACRGTELDDGIQADSGPINDT DANPRYKIPVEADFLFAYSTVPGYYSWRSPGRG SWFVQALCSILEEHGKDLEIMQILTRVNDRVAR HFESQSDDPHFHEKKQIPCVVSMLTKELYFSQ*P YQGYILAEKQWVTH**ITFFYALEIFRNSPGF* FQENVLIQQGRNFLVLSFVL*IFRDFFYNVIHL VTV*LSLKINFLFVCLLPC**T*YMQQK*LLEKA HGCVHCNWW*QW*SHVCTWQKESQCLTKHSQGD IYCSLLQNVGIECDLNDFSLA*GRFSCKSSHMS *RRKSLMILICIHQDPVWKTETILGVSTEGV*YR KLTYIDDKREAKQQEAVTTPRAMRIMGRGLVSC VQ*WDHPEELEPWWGLPSGS*DHQWIVENGAMT RTKPLTEME*AETDKRIPWSHLSCPHIFHQHLTA QAYLEATSPRTLEEQGTVRQEKNKKWM*AWPIM *T*VITNAQQFIHSIIYSLGCQIVYVCVKQSVL ALCAKSVIALKYIWNFLDYSKPYFE*IFVTFSSI SEEEFMAKIFGTLFSRWCYLLNS**MTVFFCLI VTG*KTNVHIY*LKMWLLNS*

Sequence NM_001227

CTCCCGCGCGCGGGCTCAACTTTGTAGAGCGAGGGGCCAACTTGGCAGAGCGCGCGGCCAGCTTTGCAGAGAGCGCCCTCCAGGGACTATGCGTGCGGGG ACACGGGTCGCTTTGGGCTCTTCCACCCCTGCGGAGCGCACTACCCCGAGCCAGGGGCGGTGCAAGCCCCGCCCGGCCCTACCCAGGGCGGCTCCTCCCT CCGCAGCGCCGAGACTTTTAGTTTCGCTTTCGCTAAAGGGGCCCCAGACCCTTGCTGCGGAGCGACGGAGAGAGACTGTGCCAGTCCCAGCCGCCCTACC GCCGTGGGAACGATGGCAGATGATCAGGGCTGTATTGAAGAGCAGGGGGTTGAGGATTCAGCAAATGAAGATTCAGTGGATGCTAAGCCAGACCGGTCCT CGTTTGTACCGTCCCTCTTCAGTAAGAAGAAGAAAAATGTCACCATGCGATCCATCAAGACCACCCGGGACCGAGTGCCTACATATCAGTACAACATGAA TTTTGAAAAGCTGGGCAAATGCATCATAATAAACAACAAGAACTTTGATAAAGTGACAGGTATGGGCGTTCGAAACGGAACAGACAAAGATGCCGAGGCG CTCTTCAAGTGCTTCCGAAGCCTGGGTTTTGACGTGATTGTCTATAATGACTGCTCTTGTGCCAAGATGCAAGATCTGCTTAAAAAAGCTTCTGAAGAGG ACCATACAAATGCCGCCTGCTTCGCCTGCATCCTCTTAAGCCATGGAGAAGAAAATGTAATTTATGGGAAAGATGGTGTCACACCAATAAAGGATTTGAC AGCCCACTTTAGGGGGGATAGATGCAAAACCCTTTTAGAGAAACCCAAACTCTTCTTCATTCAGGCTTGCCGAGGGACCGAGCTTGATGATGGCATCCAG GCCGACTCGGGGCCCATCAATGACACAGATGCTAATCCTCGATACAAGATCCCAGTGGAAGCTGACTTCCTCTTCGCCTATTCCACGGTTCCAGGCTATT ACTCGTGGAGGAGCCCAGGAAGAGGCTCCTGGTTTGTGCAAGCCCTCTGCTCCATCCTGGAGGAGCACGGAAAAGACCTGGAAATCATGCAGATCCTCAC CAGGGTGAATGACAGAGTTGCCAGGCACTTTGAGTCTCAGTCTGATGACCCACACTTCCATGAGAAGAAGCAGATCCCCTGTGTGGTCTCCATGCTCACC AAGGAACTCTACTTCAGTCAATAGCCATATCAGGGGTACATTCTAGCTGAGAAGCAATGGGTCACTCATTAATGAATCACATTTTTTTATGCTCTTGAAA TATTCAGAAATTCTCCAGGATTTTAATTTCAGGAAAATGTATTGATTCAACAGGGAAGAAACTTTCTGGTGCTGTCTTTTGTTCTCTGAATTTTCAGAGA CTTTTTTTATAATGTTATTCATTTGGTGACTGTGTAACTTTCTCTTAAGATTAATTTTCTCTTTGTATGTCTGTTACCTTGTTAATAGACTTAATACATG CAACAGAAGTGACTTCTGGAGAAAGCTCATGGCTGTGTCCACTGCAATTGGTGGTAACAGTGGTAGAGTCATGTTTGCACTTGGCAAAAAGAATCCCAAT GTTTGACAAAACACAGCCAAGGGGATATTTACTGCTCTTTATTGCAGAATGTGGGTATTGAGTGTGATTTGAATGATTTTTCATTGGCTTAGGGCAGATT TTCATGCAAAAGTTCTCATATGAGTTAGAGGAGAAAAAGCTTAATGATTCTGATATGTATCCATCAGGATCCAGTCTGGAAAACAGAAACCATTCTAGGT GTTTCAACAGAGGGAGTTTAATACAGGAAATTGACTTACATAGATGATAAAAGAGAAGCCAAACAGCAAGAAGCTGTTACCACACCCAGGGCTATGAGGA TAATGGGAAGAGGTTTGGTTTCCTGTGTCCAGTAGTGGGATCATCCAGAGGAGCTGGAACCATGGTGGGGGCTGCCTAGTGGGAGTTAGGACCACCAATG GATTGTGGAAAATGGAGCCATGACAAGAACAAAGCCACTGACTGAGATGGAGTGAGCTGAGACAGATAAGAGAATACCTTGGTCTCACCTATCCTGCCCT CACATCTTCCACCAGCACCTTACTGCCCAGGCCTATCTGGAAGCCACCTCACCAAGGACCTTGGAAGAGCAAGGGACAGTGAGGCAGGAGAAGAACAAGA AATGGATGTAAGCCTGGCCCATAATGTGAACATAAGTAATCACTAATGCTCAACAATTTATCCATTCAATCATTTATTCATTGGGTTGTCAGATAGTCTA TGTATGTGTAAAACAATCTGTTTTGGCTTTATGTGCAAAATCTGTTATAGCTTTAAAATATATCTGGAACTTTTTAGATTATTCCAAGCCTTATTTTGAG TAAATATTTGTTACTTTTAGTTCTATAAGTGAGGAAGAGTTTATGGCAAAGATTTTTGGCACTTTGTTTTCAAGATGGTGTTATCTTTTGAATTCTTGAT AAATGACTGTTTTTTTCTGCCTAATAGTAACTGGTTAAAAAACAAATGTTCATATTTATTGATTAAAAATGTGGTTGCTTAATTCCTAA

refseq_CASP7.F1 (forward primer)
refseq_CASP7.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

LPRAGSTL*SEGPTWQSARPALQRAPSRDYACG DTGRFGLFHPCGAHYPEPGAVQAPPGPTQGGSSL RSAETFSFAFAKGAPDPCCGATERDCASPSRPT AVGTMADDQGCIEEQGVEDSANEDSVDAKPDRS SFVPSLFSKKKKNVTMRSIKTTRDRVPTYQYNMN FEKLGKCIIINNKNFDKVTGMGVRNGTDKDAEA LFKCFRSLGFDVIVYNDCSCAKMQDLLKKASEE DHTNAACFACILLSHGEENVIYGKDGVTPIKDLT AHFRGDRCKTLLEKPKLFFIQACRGTELDDGIQ ADSGPINDTDANPRYKIPVEADFLFAYSTVPGY YSWRSPGRGSWFVQALCSILEEHGKDLEIMQILT RVNDRVARHFESQSDDPHFHEKKQIPCVVSMLT KELYFSQ*PYQGYILAEKQWVTH**ITFFYALE IFRNSPGF*FQENVLIQQGRNFLVLSFVL*IFRD FFYNVIHLVTV*LSLKINFLFVCLLPC**T*YM QQK*LLEKAHGCVHCNWW*QW*SHVCTWQKESQ CLTKHSQGDIYCSLLQNVGIECDLNDFSLA*GRF SCKSSHMS*RRKSLMILICIHQDPVWKTETILG VSTEGV*YRKLTYIDDKREAKQQEAVTTPRAMR IMGRGLVSCVQ*WDHPEELEPWWGLPSGS*DHQW IVENGAMTRTKPLTEME*AETDKRIPWSHLSCP HIFHQHLTAQAYLEATSPRTLEEQGTVRQEKNK KWM*AWPIM*T*VITNAQQFIHSIIYSLGCQIVY VCVKQSVLALCAKSVIALKYIWNFLDYSKPYFE *IFVTFSSISEEEFMAKIFGTLFSRWCYLLNS* *MTVFFCLIVTG*KTNVHIY*LKMWLLNS*

Frame 1

SRARAQLCRARGQLGRARGQLCRERPPGTMRAG TRVALGSSTPAERTTPSQGRCKPRPALPRAAPP SAAPRLLVSLSLKGPQTLAAERRRETVPVPAALP PWERWQMIRAVLKSRGLRIQQMKIQWMLSQTGP RLYRPSSVRRRKMSPCDPSRPPGTECLHISTT* ILKSWANAS**TTRTLIK*QVWAFETEQTKMPRR SSSASEAWVLT*LSIMTALVPRCKICLKKLLKR TIQMPPASPASS*AMEKKM*FMGKMVSHQ*RI* QPTLGGIDAKPF*RNPNSSSFRLAEGPSLMMASR PTRGPSMTQMLILDTRSQWKLTSSSPIPRFQAI TRGGAQEEAPGLCKPSAPSWRSTEKTWKSCRSS PG*MTELPGTLSLSLMTHTSMRRSRSPVWSPCSP RNSTSVNSHIRGTF*LRSNGSLINESHFFMLLK YSEILQDFNFRKMY*FNREETFWCCLLFSEFSE TFFIMLFIW*LCNFLLRLIFSLYVCYLVNRLNTC NRSDFWRKLMAVSTAIGGNSGRVMFALGKKNPN V*QNTAKGIFTALYCRMWVLSVI*MIFHWLRAD FHAKVLI*VRGEKA**F*YVSIRIQSGKQKPF*V FQQREFNTGN*LT*MIKEKPNSKKLLPHPGL*G *WEEVWFPVSSSGIIQRSWNHGGGCLVGVRTTN GLWKMEP*QEQSH*LRWSELRQIREYLGLTYPAL TSSTSTLLPRPIWKPPHQGPWKSKGQ*GRRRTR NGCKPGP*CEHK*SLMLNNLSIQSFIHWVVR*S MYV*NNLFWLYVQNLL*L*NISGTF*IIPSLILS KYLLLLVL*VRKSLWQRFLALCFQDGVIF*ILD K*LFFSA***LVKKQMFIFID*KCGCLIP

Frame 2

PARGLNFVERGANLAERAASFAESALQGLCVRG HGSLWALPPLRSALPRARGGASPARPYPGRLLP PQRRDF*FRFR*RGPRPLLRSDGERLCQSQPPY RRGNDGR*SGLY*RAGG*GFSK*RFSGC*ARPVL VCTVPLQ*EEEKCHHAIHQDHPGPSAYISVQHE F*KAGQMHHNKQQEL**SDRYGRSKRNRQRCRG ALQVLPKPGF*RDCL**LLLCQDARSA*KSF*RG PYKCRLLRLHPLKPWRRKCNLWERWCHTNKGFD SPL*GG*MQNPFRETQTLLHSGLPRDRA**WHP GRLGAHQ*HRC*SSIQDPSGS*LPLRLFHGSRLL LVEEPRKRLLVCASPLLHPGGARKRPGNHADPH QGE*QSCQAL*VSV**PTLP*EEADPLCGLHAH QGTLLQSIAISGVHSS*EAMGHSLMNHIFLCS*N IQKFSRILISGKCIDSTGKKLSGAVFCSLNFQR LFL*CYSFGDCVTFS*D*FSLCMSVTLLIDLIH ATEVTSGESSWLCPLQLVVTVVESCLHLAKRIPM FDKTQPRGYLLLFIAECGY*V*FE*FFIGLGQI FMQKFSYELEEKKLNDSDMYPSGSSLENRNHSR CFNRGSLIQEIDLHR**KRSQTARSCYHTQGYED NGKRFGFLCPVVGSSRGAGTMVGAA*WELGPPM DCGKWSHDKNKATD*DGVS*DR*ENTLVSPILP SHLPPAPYCPGLSGSHLTKDLGRARDSEAGEEQE MDVSLAHNVNISNH*CSTIYPFNHLFIGLSDSL CMCKTICFGFMCKICYSFKIYLELFRLFQALF* VNICYF*FYK*GRVYGKDFWHFVFKMVLSFEFLI NDCFFLPNSNWLKNKCSYLLIKNVVA*FL

Sequence NM_033338

CTCCCGCGCGCGGGCTCAACTTTGTAGAGCGAGGGGCCAACTTGGCAGAGCGCGCGGCCAGCTTTGCAGAGAGCGCCCTCCAGGGACTATGCGTGCGGGG ACACGGGTCGCTTTGGGCTCTTCCACCCCTGCGGAGCGCACTACCCCGAGCCAGGGGCGGTGCAAGCCCCGCCCGGCCCTACCCAGGGCGGCTCCTCCCT CCGCAGCGCCGAGACTTTTAGTTTCGCTTTCGCTAAAGGGGCCCCAGACCCTTGCTGCGGAGCGACGGAGAGAGACTGTGCCAGTCCCAGCCGCCCTACC GCCGTGGGAACGATGCTGTAATGGACTGTGTTGGTTGGCCTCCAGGCAGGAAGTGGCACTTGGAAAAGAACACCAGCTGCGGTGGTAGCAGTGGGATTTG TGCTTCTTATGTTACCCAGATGGCAGATGATCAGGGCTGTATTGAAGAGCAGGGGGTTGAGGATTCAGCAAATGAAGATTCAGTGGATGCTAAGCCAGAC CGGTCCTCGTTTGTACCGTCCCTCTTCAGTAAGAAGAAGAAAAATGTCACCATGCGATCCATCAAGACCACCCGGGACCGAGTGCCTACATATCAGTACA ACATGAATTTTGAAAAGCTGGGCAAATGCATCATAATAAACAACAAGAACTTTGATAAAGTGACAGGTATGGGCGTTCGAAACGGAACAGACAAAGATGC CGAGGCGCTCTTCAAGTGCTTCCGAAGCCTGGGTTTTGACGTGATTGTCTATAATGACTGCTCTTGTGCCAAGATGCAAGATCTGCTTAAAAAAGCTTCT GAAGAGGACCATACAAATGCCGCCTGCTTCGCCTGCATCCTCTTAAGCCATGGAGAAGAAAATGTAATTTATGGGAAAGATGGTGTCACACCAATAAAGG ATTTGACAGCCCACTTTAGGGGGGATAGATGCAAAACCCTTTTAGAGAAACCCAAACTCTTCTTCATTCAGGCTTGCCGAGGGACCGAGCTTGATGATGG CATCCAGGCCGACTCGGGGCCCATCAATGACACAGATGCTAATCCTCGATACAAGATCCCAGTGGAAGCTGACTTCCTCTTCGCCTATTCCACGGTTCCA GGCTATTACTCGTGGAGGAGCCCAGGAAGAGGCTCCTGGTTTGTGCAAGCCCTCTGCTCCATCCTGGAGGAGCACGGAAAAGACCTGGAAATCATGCAGA TCCTCACCAGGGTGAATGACAGAGTTGCCAGGCACTTTGAGTCTCAGTCTGATGACCCACACTTCCATGAGAAGAAGCAGATCCCCTGTGTGGTCTCCAT GCTCACCAAGGAACTCTACTTCAGTCAATAGCCATATCAGGGGTACATTCTAGCTGAGAAGCAATGGGTCACTCATTAATGAATCACATTTTTTTATGCT CTTGAAATATTCAGAAATTCTCCAGGATTTTAATTTCAGGAAAATGTATTGATTCAACAGGGAAGAAACTTTCTGGTGCTGTCTTTTGTTCTCTGAATTT TCAGAGACTTTTTTTATAATGTTATTCATTTGGTGACTGTGTAACTTTCTCTTAAGATTAATTTTCTCTTTGTATGTCTGTTACCTTGTTAATAGACTTA ATACATGCAACAGAAGTGACTTCTGGAGAAAGCTCATGGCTGTGTCCACTGCAATTGGTGGTAACAGTGGTAGAGTCATGTTTGCACTTGGCAAAAAGAA TCCCAATGTTTGACAAAACACAGCCAAGGGGATATTTACTGCTCTTTATTGCAGAATGTGGGTATTGAGTGTGATTTGAATGATTTTTCATTGGCTTAGG GCAGATTTTCATGCAAAAGTTCTCATATGAGTTAGAGGAGAAAAAGCTTAATGATTCTGATATGTATCCATCAGGATCCAGTCTGGAAAACAGAAACCAT TCTAGGTGTTTCAACAGAGGGAGTTTAATACAGGAAATTGACTTACATAGATGATAAAAGAGAAGCCAAACAGCAAGAAGCTGTTACCACACCCAGGGCT ATGAGGATAATGGGAAGAGGTTTGGTTTCCTGTGTCCAGTAGTGGGATCATCCAGAGGAGCTGGAACCATGGTGGGGGCTGCCTAGTGGGAGTTAGGACC ACCAATGGATTGTGGAAAATGGAGCCATGACAAGAACAAAGCCACTGACTGAGATGGAGTGAGCTGAGACAGATAAGAGAATACCTTGGTCTCACCTATC CTGCCCTCACATCTTCCACCAGCACCTTACTGCCCAGGCCTATCTGGAAGCCACCTCACCAAGGACCTTGGAAGAGCAAGGGACAGTGAGGCAGGAGAAG AACAAGAAATGGATGTAAGCCTGGCCCATAATGTGAACATAAGTAATCACTAATGCTCAACAATTTATCCATTCAATCATTTATTCATTGGGTTGTCAGA TAGTCTATGTATGTGTAAAACAATCTGTTTTGGCTTTATGTGCAAAATCTGTTATAGCTTTAAAATATATCTGGAACTTTTTAGATTATTCCAAGCCTTA TTTTGAGTAAATATTTGTTACTTTTAGTTCTATAAGTGAGGAAGAGTTTATGGCAAAGATTTTTGGCACTTTGTTTTCAAGATGGTGTTATCTTTTGAAT TCTTGATAAATGACTGTTTTTTTCTGCCTAATAGTAACTGGTTAAAAAACAAATGTTCATATTTATTGATTAAAAATGTGGTTGCTTAATTCCTAA

refseq_CASP7.F1 (forward primer)
refseq_CASP7.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

LPRAGSTL*SEGPTWQSARPALQRAPSRDYACG DTGRFGLFHPCGAHYPEPGAVQAPPGPTQGGSSL RSAETFSFAFAKGAPDPCCGATERDCASPSRPT AVGTML*WTVLVGLQAGSGTWKRTPAAVVAVGF VLLMLPRWQMIRAVLKSRGLRIQQMKIQWMLSQT GPRLYRPSSVRRRKMSPCDPSRPPGTECLHIST T*ILKSWANAS**TTRTLIK*QVWAFETEQTKM PRRSSSASEAWVLT*LSIMTALVPRCKICLKKLL KRTIQMPPASPASS*AMEKKM*FMGKMVSHQ*R I*QPTLGGIDAKPF*RNPNSSSFRLAEGPSLMM ASRPTRGPSMTQMLILDTRSQWKLTSSSPIPRFQ AITRGGAQEEAPGLCKPSAPSWRSTEKTWKSCR SSPG*MTELPGTLSLSLMTHTSMRRSRSPVWSP CSPRNSTSVNSHIRGTF*LRSNGSLINESHFFML LKYSEILQDFNFRKMY*FNREETFWCCLLFSEF SETFFIMLFIW*LCNFLLRLIFSLYVCYLVNRL NTCNRSDFWRKLMAVSTAIGGNSGRVMFALGKKN PNV*QNTAKGIFTALYCRMWVLSVI*MIFHWLR ADFHAKVLI*VRGEKA**F*YVSIRIQSGKQKP F*VFQQREFNTGN*LT*MIKEKPNSKKLLPHPGL *G*WEEVWFPVSSSGIIQRSWNHGGGCLVGVRT TNGLWKMEP*QEQSH*LRWSELRQIREYLGLTY PALTSSTSTLLPRPIWKPPHQGPWKSKGQ*GRRR TRNGCKPGP*CEHK*SLMLNNLSIQSFIHWVVR *SMYV*NNLFWLYVQNLL*L*NISGTF*IIPSL ILSKYLLLLVL*VRKSLWQRFLALCFQDGVIF*I LDK*LFFSA***LVKKQMFIFID*KCGCLIP

Frame 1

SRARAQLCRARGQLGRARGQLCRERPPGTMRAG TRVALGSSTPAERTTPSQGRCKPRPALPRAAPP SAAPRLLVSLSLKGPQTLAAERRRETVPVPAALP PWERCCNGLCWLASRQEVALGKEHQLRW*QWDL CFLCYPDGR*SGLY*RAGG*GFSK*RFSGC*AR PVLVCTVPLQ*EEEKCHHAIHQDHPGPSAYISVQ HEF*KAGQMHHNKQQEL**SDRYGRSKRNRQRC RGALQVLPKPGF*RDCL**LLLCQDARSA*KSF *RGPYKCRLLRLHPLKPWRRKCNLWERWCHTNKG FDSPL*GG*MQNPFRETQTLLHSGLPRDRA**W HPGRLGAHQ*HRC*SSIQDPSGS*LPLRLFHGS RLLLVEEPRKRLLVCASPLLHPGGARKRPGNHAD PHQGE*QSCQAL*VSV**PTLP*EEADPLCGLH AHQGTLLQSIAISGVHSS*EAMGHSLMNHIFLC S*NIQKFSRILISGKCIDSTGKKLSGAVFCSLNF QRLFL*CYSFGDCVTFS*D*FSLCMSVTLLIDL IHATEVTSGESSWLCPLQLVVTVVESCLHLAKR IPMFDKTQPRGYLLLFIAECGY*V*FE*FFIGLG QIFMQKFSYELEEKKLNDSDMYPSGSSLENRNH SRCFNRGSLIQEIDLHR**KRSQTARSCYHTQG YEDNGKRFGFLCPVVGSSRGAGTMVGAA*WELGP PMDCGKWSHDKNKATD*DGVS*DR*ENTLVSPI LPSHLPPAPYCPGLSGSHLTKDLGRARDSEAGE EQEMDVSLAHNVNISNH*CSTIYPFNHLFIGLSD SLCMCKTICFGFMCKICYSFKIYLELFRLFQAL F*VNICYF*FYK*GRVYGKDFWHFVFKMVLSFE FLINDCFFLPNSNWLKNKCSYLLIKNVVA*FL

Frame 2

PARGLNFVERGANLAERAASFAESALQGLCVRG HGSLWALPPLRSALPRARGGASPARPYPGRLLP PQRRDF*FRFR*RGPRPLLRSDGERLCQSQPPY RRGNDAVMDCVGWPPGRKWHLEKNTSCGGSSGIC ASYVTQMADDQGCIEEQGVEDSANEDSVDAKPD RSSFVPSLFSKKKKNVTMRSIKTTRDRVPTYQY NMNFEKLGKCIIINNKNFDKVTGMGVRNGTDKDA EALFKCFRSLGFDVIVYNDCSCAKMQDLLKKAS EEDHTNAACFACILLSHGEENVIYGKDGVTPIK DLTAHFRGDRCKTLLEKPKLFFIQACRGTELDDG IQADSGPINDTDANPRYKIPVEADFLFAYSTVP GYYSWRSPGRGSWFVQALCSILEEHGKDLEIMQ ILTRVNDRVARHFESQSDDPHFHEKKQIPCVVSM LTKELYFSQ*PYQGYILAEKQWVTH**ITFFYA LEIFRNSPGF*FQENVLIQQGRNFLVLSFVL*I FRDFFYNVIHLVTV*LSLKINFLFVCLLPC**T* YMQQK*LLEKAHGCVHCNWW*QW*SHVCTWQKE SQCLTKHSQGDIYCSLLQNVGIECDLNDFSLA* GRFSCKSSHMS*RRKSLMILICIHQDPVWKTETI LGVSTEGV*YRKLTYIDDKREAKQQEAVTTPRA MRIMGRGLVSCVQ*WDHPEELEPWWGLPSGS*D HQWIVENGAMTRTKPLTEME*AETDKRIPWSHLS CPHIFHQHLTAQAYLEATSPRTLEEQGTVRQEK NKKWM*AWPIM*T*VITNAQQFIHSIIYSLGCQ IVYVCVKQSVLALCAKSVIALKYIWNFLDYSKPY FE*IFVTFSSISEEEFMAKIFGTLFSRWCYLLN S**MTVFFCLIVTG*KTNVHIY*LKMWLLNS*