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Sequences

Sequence NM_001877

CACAGCTGCTTGCTGCTCCAGCCTTGCCCTCCCAGAGCTGCCGGACGCTCGCGGGTCTCGGAACGCATCCCGCCGCGGGGGCTTCGGCCGTGGCATGGGC GCCGCGGGCCTGCTCGGGGTTTTCTTGGCTCTCGTCGCACCGGGGGTCCTCGGGATTTCTTGTGGCTCTCCTCCGCCTATCCTAAATGGCCGGATTAGTT ATTATTCTACCCCCATTGCTGTTGGTACCGTGATAAGGTACAGTTGTTCAGGTACCTTCCGCCTCATTGGAGAAAAAAGTCTATTATGCATAACTAAAGA CAAAGTGGATGGAACCTGGGATAAACCTGCTCCTAAATGTGAATATTTCAATAAATATTCTTCTTGCCCTGAGCCCATAGTACCAGGAGGATACAAAATT AGAGGCTCTACACCCTACAGACATGGTGATTCTGTGACATTTGCCTGTAAAACCAACTTCTCCATGAACGGAAACAAGTCTGTTTGGTGTCAAGCAAATA ATATGTGGGGGCCGACACGACTACCAACCTGTGTAAGTGTTTTCCCTCTCGAGTGTCCAGCACTTCCTATGATCCACAATGGACATCACACAAGTGAGAA TGTTGGCTCCATTGCTCCAGGATTGTCTGTGACTTACAGCTGTGAATCTGGTTACTTGCTTGTTGGAGAAAAGATCATTAACTGTTTGTCTTCGGGAAAA TGGAGTGCTGTCCCCCCCACATGTGAAGAGGCACGCTGTAAATCTCTAGGACGATTTCCCAATGGGAAGGTAAAGGAGCCTCCAATTCTCCGGGTTGGTG TAACTGCAAACTTTTTCTGTGATGAAGGGTATCGACTGCAAGGCCCACCTTCTAGTCGGTGTGTAATTGCTGGACAGGGAGTTGCTTGGACCAAAATGCC AGTATGTGAAGAAATTTTTTGCCCATCACCTCCCCCTATTCTCAATGGAAGACATATAGGCAACTCACTAGCAAATGTCTCATATGGAAGCATAGTCACT TACACTTGTGACCCGGACCCAGAGGAAGGAGTGAACTTCATCCTTATTGGAGAGAGCACTCTCCGTTGTACAGTTGATAGTCAGAAGACTGGGACCTGGA GTGGCCCTGCCCCACGCTGTGAACTTTCTACTTCTGCGGTTCAGTGTCCACATCCCCAGATCCTAAGAGGCCGAATGGTATCTGGGCAGAAAGATCGATA TACCTATAACGACACTGTGATATTTGCTTGCATGTTTGGCTTCACCTTGAAGGGCAGCAAGCAAATCCGATGCAATGCCCAAGGCACATGGGAGCCATCT GCACCAGTCTGTGAAAAGGAATGCCAGGCCCCTCCTAACATCCTCAATGGGCAAAAGGAAGATAGACACATGGTCCGCTTTGACCCTGGAACATCTATAA AATATAGCTGTAACCCTGGCTATGTGCTGGTGGGAGAAGAATCCATACAGTGTACCTCTGAGGGGGTGTGGACACCCCCTGTACCCCAATGCAAAGTGGC AGCGTGTGAAGCTACAGGAAGGCAACTCTTGACAAAACCCCAGCACCAATTTGTTAGACCAGATGTCAACTCTTCTTGTGGTGAAGGGTACAAGTTAAGT GGGAGTGTTTATCAGGAGTGTCAAGGCACAATTCCTTGGTTTATGGAGATTCGTCTTTGTAAAGAAATCACCTGCCCACCACCCCCTGTTATCTACAATG GGGCACACACCGGGAGTTCCTTAGAAGATTTTCCATATGGAACCACGGTCACTTACACATGTAACCCTGGGCCAGAAAGAGGAGTGGAATTCAGCCTCAT TGGAGAGAGCACCATCCGTTGTACAAGCAATGATCAAGAAAGAGGCACCTGGAGTGGCCCTGCTCCCCTGTGTAAACTTTCCCTCCTTGCTGTCCAGTGC TCACATGTCCATATTGCAAATGGATACAAGATATCTGGCAAGGAAGCCCCATATTTCTACAATGACACTGTGACATTCAAGTGTTATAGTGGATTTACTT TGAAGGGCAGTAGTCAGATTCGTTGCAAAGCTGATAACACCTGGGATCCTGAAATACCAGTTTGTGAAAAAGAAACATGCCAGCATGTGAGACAGAGTCT TCAAGAACTTCCAGCTGGTTCACGTGTGGAGCTAGTTAATACGTCCTGCCAAGATGGGTACCAGTTGACTGGACATGCTTATCAGATGTGTCAAGATGCT GAAAATGGAATTTGGTTCAAAAAGATTCCACTTTGTAAAGTTATTCACTGTCACCCTCCACCAGTGATTGTCAATGGGAAGCACACAGGCATGATGGCAG AAAACTTTCTATATGGAAATGAAGTCTCTTATGAATGTGACCAAGGATTCTATCTCCTGGGAGAGAAAAAATTGCAGTGCAGAAGTGATTCTAAAGGACA TGGATCTTGGAGCGGGCCTTCCCCACAGTGCTTACGATCTCCTCCTGTGACTCGCTGCCCTAATCCAGAAGTCAAACATGGGTACAAGCTCAATAAAACA CATTCTGCATATTCCCACAATGACATAGTGTATGTTGACTGCAATCCTGGCTTCATCATGAATGGTAGTCGCGTGATTAGGTGTCATACTGATAACACAT GGGTGCCAGGTGTGCCAACTTGTATCAAAAAAGCCTTCATAGGGTGTCCACCTCCGCCTAAGACCCCTAACGGGAACCATACTGGTGGAAACATAGCTCG ATTTTCTCCTGGAATGTCAATCCTGTACAGCTGTGACCAAGGCTACCTGCTGGTGGGAGAGGCACTCCTTCTTTGCACACATGAGGGAACCTGGAGCCAA CCTGCCCCTCATTGTAAAGAGGTAAACTGTAGCTCACCAGCAGATATGGATGGAATCCAGAAAGGGCTGGAACCAAGGAAAATGTATCAGTATGGAGCTG TTGTAACTCTGGAGTGTGAAGATGGGTATATGCTGGAAGGCAGTCCCCAGAGCCAGTGCCAATCGGATCACCAATGGAACCCTCCCCTGGCGGTTTGCAG ATCCCGTTCACTTGCTCCTGTCCTTTGTGGTATTGCTGCAGGTTTGATACTTCTTACCTTCTTGATTGTCATTACCTTATACGTGATATCAAAACACAGA GCACGCAATTATTATACAGATACAAGCCAGAAAGAAGCTTTTCATTTAGAAGCACGAGAAGTATATTCTGTTGATCCATACAACCCAGCCAGCTGATCAG AAGACAAACTGGTGTGTGCCTCATTGCTTGGAATTCAGCGGAATATTGATTAGAAAGAAACTGCTCTAATATCAGCAAGTCTCTTTATATGGCCTCAAGA TCAATGAAATGATGTCATAAGCGATCACTTCCTATATGCACTTATTCTCAAGAAGAACATCTTTATGGTAAAGATGGGAGCCCAGTTTCACTGCCATATA CTCTTCAAGGACTTTCTGAAGCCTCACTTATGAGATGCCTGAAGCCAGGCCATGGCTATAAACAATTACATGGCTCTAAAAAGTTTTGCCCTTTTTAAGG AAGGCACTAAAAAGAGCTGTCCTGGTATCTAGACCCATCTTCTTTTTGAAATCAGCATACTCAATGTTACTATCTGCTTTTGGTTATAATGTGTTTTTAA TTATCTAAAGTATGAAGCATTTTCTGGGGTTATGATGGCTTTACCTTTATTAGGAAGTATGGTTTTATTTTGATAGTAGCTTCCTCCTCTGGTGGTGTTA ATCATTTCATTTTTACCCTTACTTGGTTTGAGTTTCTCTCACATTACTGTATATACTTTGCCTTTCCATAATCACTCAGTGATTGCAATTTGCACAAGTT TTTTTAAATTATGGGAATCAAGATTTAATCCTAGAGATTTGGTGTACAATTCAGGCTTTGGATGTTTCTTTAGCAGTTTTGTGATAAGTTCTAGTTGCTT GTAAAATTTCACTTAATAATGTGTACATTAGTCATTCAATAAATTGTAATTGTAAAGAAAACATACAA

refseq_CR2.F1 (forward primer)
refseq_CR2.R1 (reverse primer)
Single exon skipping

Frame 0

HSCLLLQPCPPRAAGRSRVSERIPPRGLRPWHG RRGPARGFLGSRRTGGPRDFLWLSSAYPKWPD*L LFYPHCCWYRDKVQLFRYLPPHWRKKSIMHN*R QSGWNLG*TCS*M*IFQ*IFFLP*AHSTRRIQN *RLYTLQTW*FCDICL*NQLLHERKQVCLVSSK* YVGADTTTNLCKCFPSRVSSTSYDPQWTSHK*E CWLHCSRIVCDLQL*IWLLACWRKDH*LFVFGK MECCPPHM*RGTL*ISRTISQWEGKGASNSPGWC NCKLFL**RVSTARPTF*SVCNCWTGSCLDQNA SM*RNFLPITSPYSQWKTYRQLTSKCLIWKHSH LHL*PGPRGRSELHPYWREHSPLYS**SEDWDLE WPCPTL*TFYFCGSVSTSPDPKRPNGIWAERSI YL*RHCDICLHVWLHLEGQQANPMQCPRHMGAI CTSL*KGMPGPS*HPQWAKGR*THGPL*PWNIYK I*L*PWLCAGGRRIHTVYL*GGVDTPCTPMQSG SV*SYRKATLDKTPAPIC*TRCQLFLW*RVQVK WECLSGVSRHNSLVYGDSSL*RNHLPTTPCYLQW GTHREFLRRFSIWNHGHLHM*PWARKRSGIQPH WREHHPLYKQ*SRKRHLEWPCSPV*TFPPCCPV LTCPYCKWIQDIWQGSPIFLQ*HCDIQVL*WIYF EGQ*SDSLQS**HLGS*NTSL*KRNMPACETES SRTSSWFTCGAS*YVLPRWVPVDWTCLSDVSRC *KWNLVQKDSTL*SYSLSPSTSDCQWEAHRHDGR KLSIWK*SLL*M*PRILSPGREKIAVQK*F*RT WILERAFPTVLTISSCDSLP*SRSQTWVQAQ*N TFCIFPQ*HSVC*LQSWLHHEW*SRD*VSY**HM GARCANLYQKSLHRVSTSA*DP*REPYWWKHSS IFSWNVNPVQL*PRLPAGGRGTPSLHT*GNLEP TCPSL*RGKL*LTSRYGWNPERAGTKENVSVWSC CNSGV*RWVYAGRQSPEPVPIGSPMEPSPGGLQ IPFTCSCPLWYCCRFDTSYLLDCHYLIRDIKTQ STQLLYRYKPERSFSFRSTRSIFC*SIQPSQLIR RQTGVCLIAWNSAEY*LERNCSNISKSLYMASR SMK*CHKRSLPICTYSQEEHLYGKDGSPVSLPY TLQGLSEASLMRCLKPGHGYKQLHGSKKFCPF*G RH*KELSWYLDPSSF*NQHTQCYYLLLVIMCF* LSKV*SIFWGYDGFTFIRKYGFILIVASSSGGV NHFIFTLTWFEFLSHYCIYFAFP*SLSDCNLHKF F*IMGIKI*S*RFGVQFRLWMFL*QFCDKF*LL VKFHLIMCTLVIQ*IVIVKKTY

Frame 1

TAACCSSLALPELPDARGSRNASRRGGFGRGMG AAGLLGVFLALVAPGVLGISCGSPPPILNGRIS YYSTPIAVGTVIRYSCSGTFRLIGEKSLLCITKD KVDGTWDKPAPKCEYFNKYSSCPEPIVPGGYKI RGSTPYRHGDSVTFACKTNFSMNGNKSVWCQAN NMWGPTRLPTCVSVFPLECPALPMIHNGHHTSEN VGSIAPGLSVTYSCESGYLLVGEKIINCLSSGK WSAVPPTCEEARCKSLGRFPNGKVKEPPILRVG VTANFFCDEGYRLQGPPSSRCVIAGQGVAWTKMP VCEEIFCPSPPPILNGRHIGNSLANVSYGSIVT YTCDPDPEEGVNFILIGESTLRCTVDSQKTGTW SGPAPRCELSTSAVQCPHPQILRGRMVSGQKDRY TYNDTVIFACMFGFTLKGSKQIRCNAQGTWEPS APVCEKECQAPPNILNGQKEDRHMVRFDPGTSI KYSCNPGYVLVGEESIQCTSEGVWTPPVPQCKVA ACEATGRQLLTKPQHQFVRPDVNSSCGEGYKLS GSVYQECQGTIPWFMEIRLCKEITCPPPPVIYN GAHTGSSLEDFPYGTTVTYTCNPGPERGVEFSLI GESTIRCTSNDQERGTWSGPAPLCKLSLLAVQC SHVHIANGYKISGKEAPYFYNDTVTFKCYSGFT LKGSSQIRCKADNTWDPEIPVCEKETCQHVRQSL QELPAGSRVELVNTSCQDGYQLTGHAYQMCQDA ENGIWFKKIPLCKVIHCHPPPVIVNGKHTGMMA ENFLYGNEVSYECDQGFYLLGEKKLQCRSDSKGH GSWSGPSPQCLRSPPVTRCPNPEVKHGYKLNKT HSAYSHNDIVYVDCNPGFIMNGSRVIRCHTDNT WVPGVPTCIKKAFIGCPPPPKTPNGNHTGGNIAR FSPGMSILYSCDQGYLLVGEALLLCTHEGTWSQ PAPHCKEVNCSSPADMDGIQKGLEPRKMYQYGA VVTLECEDGYMLEGSPQSQCQSDHQWNPPLAVCR SRSLAPVLCGIAAGLILLTFLIVITLYVISKHR ARNYYTDTSQKEAFHLEAREVYSVDPYNPAS*S EDKLVCASLLGIQRNID*KETALISASLFIWPQD Q*NDVISDHFLYALILKKNIFMVKMGAQFHCHI LFKDFLKPHL*DA*SQAMAINNYMALKSFALFK EGTKKSCPGI*THLLFEISILNVTICFWL*CVFN YLKYEAFSGVMMALPLLGSMVLF***LPPLVVL IISFLPLLGLSFSHITVYTLPFHNHSVIAICTS FFKLWESRFNPRDLVYNSGFGCFFSSFVISSSCL *NFT**CVH*SFNKL*L*RKHT

Frame 2

QLLAAPALPSQSCRTLAGLGTHPAAGASAVAWA PRACSGFSWLSSHRGSSGFLVALLRLS*MAGLV IILPPLLLVP**GTVVQVPSASLEKKVYYA*LK TKWMEPGINLLLNVNISINILLALSP*YQEDTKL EALHPTDMVIL*HLPVKPTSP*TETSLFGVKQI ICGGRHDYQPV*VFSLSSVQHFL*STMDITQVR MLAPLLQDCL*LTAVNLVTCLLEKRSLTVCLREN GVLSPPHVKRHAVNL*DDFPMGR*RSLQFSGLV *LQTFSVMKGIDCKAHLLVGV*LLDRELLGPKC QYVKKFFAHHLPLFSMEDI*ATH*QMSHMEA*SL TLVTRTQRKE*TSSLLERALSVVQLIVRRLGPG VALPHAVNFLLLRFSVHIPRS*EAEWYLGRKID IPITTL*YLLACLASP*RAASKSDAMPKAHGSHL HQSVKRNARPLLTSSMGKRKIDTWSALTLEHL* NIAVTLAMCWWEKNPYSVPLRGCGHPLYPNAKW QRVKLQEGNS*QNPSTNLLDQMSTLLVVKGTS*V GVFIRSVKAQFLGLWRFVFVKKSPAHHPLLSTM GHTPGVP*KIFHMEPRSLTHVTLGQKEEWNSAS LERAPSVVQAMIKKEAPGVALLPCVNFPSLLSSA HMSILQMDTRYLARKPHISTMTL*HSSVIVDLL *RAVVRFVAKLITPGILKYQFVKKKHASM*DRV FKNFQLVHVWS*LIRPAKMGTS*LDMLIRCVKML KMEFGSKRFHFVKLFTVTLHQ*LSMGSTQA*WQ KTFYMEMKSLMNVTKDSISWERKNCSAEVILKD MDLGAGLPHSAYDLLL*LAALIQKSNMGTSSIKH ILHIPTMT*CMLTAILASS*MVVA*LGVILITH GCQVCQLVSKKPS*GVHLRLRPLTGTILVET*L DFLLECQSCTAVTKATCWWERHSFFAHMREPGAN LPLIVKR*TVAHQQIWMESRKGWNQGKCISMEL L*LWSVKMGICWKAVPRASANRITNGTLPWRFA DPVHLLLSFVVLLQV*YFLPS*LSLPYT*YQNTE HAIIIQIQARKKLFI*KHEKYILLIHTTQPADQ KTNWCVPHCLEFSGILIRKKLL*YQQVSLYGLK INEMMS*AITSYMHLFSRRTSLW*RWEPSFTAIY SSRTF*SLTYEMPEARPWL*TITWL*KVLPFLR KALKRAVLVSRPIFFLKSAYSMLLSAFGYNVFL II*SMKHFLGL*WLYLY*EVWFYFDSSFLLWWC* SFHFYPYLV*VSLTLLYILCLSIITQ*LQFAQV FLNYGNQDLILEIWCTIQALDVSLAVL**VLVA CKISLNNVYISHSINCNCKENIQ

Sequence NM_001006658

CACAGCTGCTTGCTGCTCCAGCCTTGCCCTCCCAGAGCTGCCGGACGCTCGCGGGTCTCGGAACGCATCCCGCCGCGGGGGCTTCGGCCGTGGCATGGGC GCCGCGGGCCTGCTCGGGGTTTTCTTGGCTCTCGTCGCACCGGGGGTCCTCGGGATTTCTTGTGGCTCTCCTCCGCCTATCCTAAATGGCCGGATTAGTT ATTATTCTACCCCCATTGCTGTTGGTACCGTGATAAGGTACAGTTGTTCAGGTACCTTCCGCCTCATTGGAGAAAAAAGTCTATTATGCATAACTAAAGA CAAAGTGGATGGAACCTGGGATAAACCTGCTCCTAAATGTGAATATTTCAATAAATATTCTTCTTGCCCTGAGCCCATAGTACCAGGAGGATACAAAATT AGAGGCTCTACACCCTACAGACATGGTGATTCTGTGACATTTGCCTGTAAAACCAACTTCTCCATGAACGGAAACAAGTCTGTTTGGTGTCAAGCAAATA ATATGTGGGGGCCGACACGACTACCAACCTGTGTAAGTGTTTTCCCTCTCGAGTGTCCAGCACTTCCTATGATCCACAATGGACATCACACAAGTGAGAA TGTTGGCTCCATTGCTCCAGGATTGTCTGTGACTTACAGCTGTGAATCTGGTTACTTGCTTGTTGGAGAAAAGATCATTAACTGTTTGTCTTCGGGAAAA TGGAGTGCTGTCCCCCCCACATGTGAAGAGGCACGCTGTAAATCTCTAGGACGATTTCCCAATGGGAAGGTAAAGGAGCCTCCAATTCTCCGGGTTGGTG TAACTGCAAACTTTTTCTGTGATGAAGGGTATCGACTGCAAGGCCCACCTTCTAGTCGGTGTGTAATTGCTGGACAGGGAGTTGCTTGGACCAAAATGCC AGTATGTGAAGAAATTTTTTGCCCATCACCTCCCCCTATTCTCAATGGAAGACATATAGGCAACTCACTAGCAAATGTCTCATATGGAAGCATAGTCACT TACACTTGTGACCCGGACCCAGAGGAAGGAGTGAACTTCATCCTTATTGGAGAGAGCACTCTCCGTTGTACAGTTGATAGTCAGAAGACTGGGACCTGGA GTGGCCCTGCCCCACGCTGTGAACTTTCTACTTCTGCGGTTCAGTGTCCACATCCCCAGATCCTAAGAGGCCGAATGGTATCTGGGCAGAAAGATCGATA TACCTATAACGACACTGTGATATTTGCTTGCATGTTTGGCTTCACCTTGAAGGGCAGCAAGCAAATCCGATGCAATGCCCAAGGCACATGGGAGCCATCT GCACCAGTCTGTGAAAAGGAATGCCAGGCCCCTCCTAACATCCTCAATGGGCAAAAGGAAGATAGACACATGGTCCGCTTTGACCCTGGAACATCTATAA AATATAGCTGTAACCCTGGCTATGTGCTGGTGGGAGAAGAATCCATACAGTGTACCTCTGAGGGGGTGTGGACACCCCCTGTACCCCAATGCAAAGTGGC AGCGTGTGAAGCTACAGGAAGGCAACTCTTGACAAAACCCCAGCACCAATTTGTTAGACCAGATGTCAACTCTTCTTGTGGTGAAGGGTACAAGTTAAGT GGGAGTGTTTATCAGGAGTGTCAAGGCACAATTCCTTGGTTTATGGAGATTCGTCTTTGTAAAGAAATCACCTGCCCACCACCCCCTGTTATCTACAATG GGGCACACACCGGGAGTTCCTTAGAAGATTTTCCATATGGAACCACGGTCACTTACACATGTAACCCTGGGCCAGAAAGAGGAGTGGAATTCAGCCTCAT TGGAGAGAGCACCATCCGTTGTACAAGCAATGATCAAGAAAGAGGCACCTGGAGTGGCCCTGCTCCCCTGTGTAAACTTTCCCTCCTTGCTGTCCAGTGC TCACATGTCCATATTGCAAATGGATACAAGATATCTGGCAAGGAAGCCCCATATTTCTACAATGACACTGTGACATTCAAGTGTTATAGTGGATTTACTT TGAAGGGCAGTAGTCAGATTCGTTGCAAAGCTGATAACACCTGGGATCCTGAAATACCAGTTTGTGAAAAAGGCTGCCAGTCACCTCCTGGGCTCCACCA TGGTCGTCATACAGGTGGAAATACGGTCTTCTTTGTCTCTGGGATGACTGTAGACTACACTTGTGACCCTGGCTATTTGCTTGTGGGAAACAAATCCATT CACTGTATGCCTTCAGGAAATTGGAGTCCTTCTGCCCCACGGTGTGAAGAAACATGCCAGCATGTGAGACAGAGTCTTCAAGAACTTCCAGCTGGTTCAC GTGTGGAGCTAGTTAATACGTCCTGCCAAGATGGGTACCAGTTGACTGGACATGCTTATCAGATGTGTCAAGATGCTGAAAATGGAATTTGGTTCAAAAA GATTCCACTTTGTAAAGTTATTCACTGTCACCCTCCACCAGTGATTGTCAATGGGAAGCACACAGGCATGATGGCAGAAAACTTTCTATATGGAAATGAA GTCTCTTATGAATGTGACCAAGGATTCTATCTCCTGGGAGAGAAAAAATTGCAGTGCAGAAGTGATTCTAAAGGACATGGATCTTGGAGCGGGCCTTCCC CACAGTGCTTACGATCTCCTCCTGTGACTCGCTGCCCTAATCCAGAAGTCAAACATGGGTACAAGCTCAATAAAACACATTCTGCATATTCCCACAATGA CATAGTGTATGTTGACTGCAATCCTGGCTTCATCATGAATGGTAGTCGCGTGATTAGGTGTCATACTGATAACACATGGGTGCCAGGTGTGCCAACTTGT ATCAAAAAAGCCTTCATAGGGTGTCCACCTCCGCCTAAGACCCCTAACGGGAACCATACTGGTGGAAACATAGCTCGATTTTCTCCTGGAATGTCAATCC TGTACAGCTGTGACCAAGGCTACCTGCTGGTGGGAGAGGCACTCCTTCTTTGCACACATGAGGGAACCTGGAGCCAACCTGCCCCTCATTGTAAAGAGGT AAACTGTAGCTCACCAGCAGATATGGATGGAATCCAGAAAGGGCTGGAACCAAGGAAAATGTATCAGTATGGAGCTGTTGTAACTCTGGAGTGTGAAGAT GGGTATATGCTGGAAGGCAGTCCCCAGAGCCAGTGCCAATCGGATCACCAATGGAACCCTCCCCTGGCGGTTTGCAGATCCCGTTCACTTGCTCCTGTCC TTTGTGGTATTGCTGCAGGTTTGATACTTCTTACCTTCTTGATTGTCATTACCTTATACGTGATATCAAAACACAGAGCACGCAATTATTATACAGATAC AAGCCAGAAAGAAGCTTTTCATTTAGAAGCACGAGAAGTATATTCTGTTGATCCATACAACCCAGCCAGCTGATCAGAAGACAAACTGGTGTGTGCCTCA TTGCTTGGAATTCAGCGGAATATTGATTAGAAAGAAACTGCTCTAATATCAGCAAGTCTCTTTATATGGCCTCAAGATCAATGAAATGATGTCATAAGCG ATCACTTCCTATATGCACTTATTCTCAAGAAGAACATCTTTATGGTAAAGATGGGAGCCCAGTTTCACTGCCATATACTCTTCAAGGACTTTCTGAAGCC TCACTTATGAGATGCCTGAAGCCAGGCCATGGCTATAAACAATTACATGGCTCTAAAAAGTTTTGCCCTTTTTAAGGAAGGCACTAAAAAGAGCTGTCCT GGTATCTAGACCCATCTTCTTTTTGAAATCAGCATACTCAATGTTACTATCTGCTTTTGGTTATAATGTGTTTTTAATTATCTAAAGTATGAAGCATTTT CTGGGGTTATGATGGCTTTACCTTTATTAGGAAGTATGGTTTTATTTTGATAGTAGCTTCCTCCTCTGGTGGTGTTAATCATTTCATTTTTACCCTTACT TGGTTTGAGTTTCTCTCACATTACTGTATATACTTTGCCTTTCCATAATCACTCAGTGATTGCAATTTGCACAAGTTTTTTTAAATTATGGGAATCAAGA TTTAATCCTAGAGATTTGGTGTACAATTCAGGCTTTGGATGTTTCTTTAGCAGTTTTGTGATAAGTTCTAGTTGCTTGTAAAATTTCACTTAATAATGTG TACATTAGTCATTCAATAAATTGTAATTGTAAAGAAAACATACAA

refseq_CR2.F1 (forward primer)
refseq_CR2.R1 (reverse primer)
Single exon skipping

Frame 0

HSCLLLQPCPPRAAGRSRVSERIPPRGLRPWHG RRGPARGFLGSRRTGGPRDFLWLSSAYPKWPD*L LFYPHCCWYRDKVQLFRYLPPHWRKKSIMHN*R QSGWNLG*TCS*M*IFQ*IFFLP*AHSTRRIQN *RLYTLQTW*FCDICL*NQLLHERKQVCLVSSK* YVGADTTTNLCKCFPSRVSSTSYDPQWTSHK*E CWLHCSRIVCDLQL*IWLLACWRKDH*LFVFGK MECCPPHM*RGTL*ISRTISQWEGKGASNSPGWC NCKLFL**RVSTARPTF*SVCNCWTGSCLDQNA SM*RNFLPITSPYSQWKTYRQLTSKCLIWKHSH LHL*PGPRGRSELHPYWREHSPLYS**SEDWDLE WPCPTL*TFYFCGSVSTSPDPKRPNGIWAERSI YL*RHCDICLHVWLHLEGQQANPMQCPRHMGAI CTSL*KGMPGPS*HPQWAKGR*THGPL*PWNIYK I*L*PWLCAGGRRIHTVYL*GGVDTPCTPMQSG SV*SYRKATLDKTPAPIC*TRCQLFLW*RVQVK WECLSGVSRHNSLVYGDSSL*RNHLPTTPCYLQW GTHREFLRRFSIWNHGHLHM*PWARKRSGIQPH WREHHPLYKQ*SRKRHLEWPCSPV*TFPPCCPV LTCPYCKWIQDIWQGSPIFLQ*HCDIQVL*WIYF EGQ*SDSLQS**HLGS*NTSL*KRLPVTSWAPP WSSYRWKYGLLCLWDDCRLHL*PWLFACGKQIH SLYAFRKLESFCPTV*RNMPACETESSRTSSWFT CGAS*YVLPRWVPVDWTCLSDVSRC*KWNLVQK DSTL*SYSLSPSTSDCQWEAHRHDGRKLSIWK* SLL*M*PRILSPGREKIAVQK*F*RTWILERAFP TVLTISSCDSLP*SRSQTWVQAQ*NTFCIFPQ* HSVC*LQSWLHHEW*SRD*VSY**HMGARCANL YQKSLHRVSTSA*DP*REPYWWKHSSIFSWNVNP VQL*PRLPAGGRGTPSLHT*GNLEPTCPSL*RG KL*LTSRYGWNPERAGTKENVSVWSCCNSGV*R WVYAGRQSPEPVPIGSPMEPSPGGLQIPFTCSCP LWYCCRFDTSYLLDCHYLIRDIKTQSTQLLYRY KPERSFSFRSTRSIFC*SIQPSQLIRRQTGVCL IAWNSAEY*LERNCSNISKSLYMASRSMK*CHKR SLPICTYSQEEHLYGKDGSPVSLPYTLQGLSEA SLMRCLKPGHGYKQLHGSKKFCPF*GRH*KELS WYLDPSSF*NQHTQCYYLLLVIMCF*LSKV*SIF WGYDGFTFIRKYGFILIVASSSGGVNHFIFTLT WFEFLSHYCIYFAFP*SLSDCNLHKFF*IMGIK I*S*RFGVQFRLWMFL*QFCDKF*LLVKFHLIMC TLVIQ*IVIVKKTY

Frame 1

TAACCSSLALPELPDARGSRNASRRGGFGRGMG AAGLLGVFLALVAPGVLGISCGSPPPILNGRIS YYSTPIAVGTVIRYSCSGTFRLIGEKSLLCITKD KVDGTWDKPAPKCEYFNKYSSCPEPIVPGGYKI RGSTPYRHGDSVTFACKTNFSMNGNKSVWCQAN NMWGPTRLPTCVSVFPLECPALPMIHNGHHTSEN VGSIAPGLSVTYSCESGYLLVGEKIINCLSSGK WSAVPPTCEEARCKSLGRFPNGKVKEPPILRVG VTANFFCDEGYRLQGPPSSRCVIAGQGVAWTKMP VCEEIFCPSPPPILNGRHIGNSLANVSYGSIVT YTCDPDPEEGVNFILIGESTLRCTVDSQKTGTW SGPAPRCELSTSAVQCPHPQILRGRMVSGQKDRY TYNDTVIFACMFGFTLKGSKQIRCNAQGTWEPS APVCEKECQAPPNILNGQKEDRHMVRFDPGTSI KYSCNPGYVLVGEESIQCTSEGVWTPPVPQCKVA ACEATGRQLLTKPQHQFVRPDVNSSCGEGYKLS GSVYQECQGTIPWFMEIRLCKEITCPPPPVIYN GAHTGSSLEDFPYGTTVTYTCNPGPERGVEFSLI GESTIRCTSNDQERGTWSGPAPLCKLSLLAVQC SHVHIANGYKISGKEAPYFYNDTVTFKCYSGFT LKGSSQIRCKADNTWDPEIPVCEKGCQSPPGLHH GRHTGGNTVFFVSGMTVDYTCDPGYLLVGNKSI HCMPSGNWSPSAPRCEETCQHVRQSLQELPAGS RVELVNTSCQDGYQLTGHAYQMCQDAENGIWFKK IPLCKVIHCHPPPVIVNGKHTGMMAENFLYGNE VSYECDQGFYLLGEKKLQCRSDSKGHGSWSGPS PQCLRSPPVTRCPNPEVKHGYKLNKTHSAYSHND IVYVDCNPGFIMNGSRVIRCHTDNTWVPGVPTC IKKAFIGCPPPPKTPNGNHTGGNIARFSPGMSI LYSCDQGYLLVGEALLLCTHEGTWSQPAPHCKEV NCSSPADMDGIQKGLEPRKMYQYGAVVTLECED GYMLEGSPQSQCQSDHQWNPPLAVCRSRSLAPV LCGIAAGLILLTFLIVITLYVISKHRARNYYTDT SQKEAFHLEAREVYSVDPYNPAS*SEDKLVCAS LLGIQRNID*KETALISASLFIWPQDQ*NDVIS DHFLYALILKKNIFMVKMGAQFHCHILFKDFLKP HL*DA*SQAMAINNYMALKSFALFKEGTKKSCP GI*THLLFEISILNVTICFWL*CVFNYLKYEAF SGVMMALPLLGSMVLF***LPPLVVLIISFLPLL GLSFSHITVYTLPFHNHSVIAICTSFFKLWESR FNPRDLVYNSGFGCFFSSFVISSSCL*NFT**C VH*SFNKL*L*RKHT

Frame 2

QLLAAPALPSQSCRTLAGLGTHPAAGASAVAWA PRACSGFSWLSSHRGSSGFLVALLRLS*MAGLV IILPPLLLVP**GTVVQVPSASLEKKVYYA*LK TKWMEPGINLLLNVNISINILLALSP*YQEDTKL EALHPTDMVIL*HLPVKPTSP*TETSLFGVKQI ICGGRHDYQPV*VFSLSSVQHFL*STMDITQVR MLAPLLQDCL*LTAVNLVTCLLEKRSLTVCLREN GVLSPPHVKRHAVNL*DDFPMGR*RSLQFSGLV *LQTFSVMKGIDCKAHLLVGV*LLDRELLGPKC QYVKKFFAHHLPLFSMEDI*ATH*QMSHMEA*SL TLVTRTQRKE*TSSLLERALSVVQLIVRRLGPG VALPHAVNFLLLRFSVHIPRS*EAEWYLGRKID IPITTL*YLLACLASP*RAASKSDAMPKAHGSHL HQSVKRNARPLLTSSMGKRKIDTWSALTLEHL* NIAVTLAMCWWEKNPYSVPLRGCGHPLYPNAKW QRVKLQEGNS*QNPSTNLLDQMSTLLVVKGTS*V GVFIRSVKAQFLGLWRFVFVKKSPAHHPLLSTM GHTPGVP*KIFHMEPRSLTHVTLGQKEEWNSAS LERAPSVVQAMIKKEAPGVALLPCVNFPSLLSSA HMSILQMDTRYLARKPHISTMTL*HSSVIVDLL *RAVVRFVAKLITPGILKYQFVKKAASHLLGST MVVIQVEIRSSLSLG*L*TTLVTLAICLWETNPF TVCLQEIGVLLPHGVKKHASM*DRVFKNFQLVH VWS*LIRPAKMGTS*LDMLIRCVKMLKMEFGSK RFHFVKLFTVTLHQ*LSMGSTQA*WQKTFYMEMK SLMNVTKDSISWERKNCSAEVILKDMDLGAGLP HSAYDLLL*LAALIQKSNMGTSSIKHILHIPTM T*CMLTAILASS*MVVA*LGVILITHGCQVCQLV SKKPS*GVHLRLRPLTGTILVET*LDFLLECQS CTAVTKATCWWERHSFFAHMREPGANLPLIVKR *TVAHQQIWMESRKGWNQGKCISMELL*LWSVKM GICWKAVPRASANRITNGTLPWRFADPVHLLLS FVVLLQV*YFLPS*LSLPYT*YQNTEHAIIIQI QARKKLFI*KHEKYILLIHTTQPADQKTNWCVPH CLEFSGILIRKKLL*YQQVSLYGLKINEMMS*A ITSYMHLFSRRTSLW*RWEPSFTAIYSSRTF*S LTYEMPEARPWL*TITWL*KVLPFLRKALKRAVL VSRPIFFLKSAYSMLLSAFGYNVFLII*SMKHF LGL*WLYLY*EVWFYFDSSFLLWWC*SFHFYPY LV*VSLTLLYILCLSIITQ*LQFAQVFLNYGNQD LILEIWCTIQALDVSLAVL**VLVACKISLNNV YISHSINCNCKENIQ