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Sequences

Sequence NM_033150

ACGCAGAGCGCTGCTGGGCTGCCGGGTCTCCCGCTTCCCCCTCCTGCTCCAAGGGCCTCCTGCATGAGGGCGCGGTAGAGACCCGGACCCGCGCCGTGCT CCTGCCGTTTCGCTGCGCTCCGCCCGGGCCCGGCTCAGCCAGGCCCCGCGGTGAGCCATGATTCGCCTCGGGGCTCCCCAGACGCTGGTGCTGCTGACGC TGCTCGTCGCCGCTGTCCTTCGGTGTCAGGGCCAGGATGTCCGGCAACCAGGACCAAAGGGACAGAAAGGAGAACCTGGAGACATCAAGGATATTGTAGG ACCCAAAGGACCTCCTGGGCCTCAGGGACCTGCAGGGGAACAAGGACCCAGAGGGGATCGTGGTGACAAAGGTGAAAAAGGTGCCCCTGGACCTCGTGGC AGAGATGGAGAACCTGGGACCCCTGGAAATCCTGGCCCCCCTGGTCCTCCCGGCCCCCCTGGTCCCCCTGGTCTTGGTGGAAACTTTGCTGCCCAGATGG CTGGAGGATTTGATGAAAAGGCTGGTGGCGCCCAGTTGGGAGTAATGCAAGGACCAATGGGCCCCATGGGACCTCGAGGACCTCCAGGCCCTGCAGGTGC TCCTGGGCCTCAAGGATTTCAAGGCAATCCTGGTGAACCTGGTGAACCTGGTGTCTCTGGTCCCATGGGTCCCCGTGGTCCTCCTGGTCCCCCTGGAAAG CCTGGTGATGATGGTGAAGCTGGAAAACCTGGAAAAGCTGGTGAAAGGGGTCCGCCTGGTCCTCAGGGTGCTCGTGGTTTCCCAGGAACCCCAGGCCTTC CTGGTGTCAAAGGTCACAGAGGTTATCCAGGCCTGGACGGTGCTAAGGGAGAGGCGGGTGCTCCTGGTGTGAAGGGTGAGAGTGGTTCCCCGGGTGAGAA CGGATCTCCGGGCCCAATGGGTCCTCGTGGCCTGCCTGGTGAAAGAGGACGGACTGGCCCTGCTGGCGCTGCGGGTGCCCGAGGCAACGATGGTCAGCCA GGCCCCGCAGGGCCTCCGGGTCCTGTCGGTCCTGCTGGTGGTCCTGGCTTCCCTGGTGCTCCTGGAGCCAAGGGTGAAGCCGGCCCCACTGGTGCCCGTG GTCCTGAAGGTGCTCAAGGTCCTCGCGGTGAACCTGGTACTCCTGGGTCCCCTGGGCCTGCTGGTGCCTCCGGTAACCCTGGAACAGATGGAATTCCTGG AGCCAAAGGATCTGCTGGTGCTCCTGGCATTGCTGGTGCTCCTGGCTTCCCTGGGCCACGGGGCCCTCCTGGCCCTCAAGGTGCAACTGGTCCTCTGGGC CCGAAAGGTCAGACGGGTGAACCTGGTATTGCTGGCTTCAAAGGTGAACAAGGCCCCAAGGGAGAACCTGGCCCTGCTGGCCCCCAGGGAGCCCCTGGAC CCGCTGGTGAAGAAGGCAAGAGAGGTGCCCGTGGAGAGCCTGGTGGCGTTGGGCCCATCGGTCCCCCTGGAGAAAGAGGTGCTCCCGGCAACCGCGGTTT CCCAGGTCAAGATGGTCTGGCAGGTCCCAAGGGAGCCCCTGGAGAGCGAGGGCCCAGTGGTCTTGCTGGCCCCAAGGGAGCCAACGGTGACCCTGGCCGT CCTGGAGAACCTGGCCTTCCTGGAGCCCGGGGTCTCACTGGCCGCCCTGGTGATGCTGGTCCTCAAGGCAAAGTTGGCCCTTCTGGAGCCCCTGGTGAAG ATGGTCGTCCTGGACCTCCAGGTCCTCAGGGGGCTCGTGGGCAGCCTGGTGTCATGGGTTTCCCTGGCCCCAAAGGTGCCAACGGTGAGCCTGGCAAAGC TGGTGAGAAGGGACTGCCTGGTGCTCCTGGTCTGAGGGGTCTTCCTGGCAAAGATGGTGAGACAGGTGCTGCAGGACCCCCTGGCCCTGCTGGACCTGCT GGTGAACGAGGCGAGCAGGGTGCTCCTGGGCCATCTGGGTTCCAGGGACTTCCTGGCCCTCCTGGTCCCCCAGGTGAAGGTGGAAAACCAGGTGACCAGG GTGTTCCCGGTGAAGCTGGAGCCCCTGGCCTCGTGGGTCCCAGGGGTGAACGAGGTTTCCCAGGTGAACGTGGCTCTCCCGGTGCCCAGGGCCTCCAGGG TCCCCGTGGCCTCCCCGGCACTCCTGGCACTGATGGTCCCAAAGGTGCATCTGGCCCAGCAGGCCCCCCTGGGGCTCAGGGCCCTCCAGGTCTTCAGGGA ATGCCTGGCGAGAGGGGAGCAGCTGGTATCGCTGGGCCCAAAGGCGACAGGGGTGACGTTGGTGAGAAAGGCCCTGAGGGAGCCCCTGGAAAGGATGGTG GACGAGGCCTGACAGGTCCCATTGGCCCCCCTGGCCCAGCTGGTGCTAATGGCGAGAAGGGAGAAGTTGGACCTCCTGGTCCTGCAGGAAGTGCTGGTGC TCGTGGCGCTCCGGGTGAACGTGGAGAGACTGGGCCCCCCGGACCAGCGGGATTTGCTGGGCCTCCTGGTGCTGATGGCCAGCCTGGGGCCAAGGGTGAG CAAGGAGAGGCCGGCCAGAAAGGCGATGCTGGTGCCCCTGGTCCTCAGGGCCCCTCTGGAGCACCTGGGCCTCAGGGTCCTACTGGAGTGACTGGTCCTA AAGGAGCCCGAGGTGCCCAAGGCCCCCCGGGAGCCACTGGATTCCCTGGAGCTGCTGGCCGCGTTGGACCCCCAGGCTCCAATGGCAACCCTGGACCCCC TGGTCCCCCTGGTCCTTCTGGAAAAGATGGTCCCAAAGGTGCTCGAGGAGACAGCGGCCCCCCTGGCCGAGCTGGTGAACCCGGCCTCCAAGGTCCTGCT GGACCCCCTGGCGAGAAGGGAGAGCCTGGAGATGACGGTCCCTCTGGTGCCGAAGGTCCACCAGGTCCCCAGGGTCTGGCTGGTCAGAGAGGCATCGTCG GTCTGCCTGGGCAACGTGGTGAGAGAGGATTCCCTGGCTTGCCTGGCCCGTCGGGTGAGCCCGGCAAGCAGGGTGCTCCTGGAGCATCTGGAGACAGAGG TCCTCCTGGCCCCGTGGGTCCTCCTGGCCTGACGGGTCCTGCAGGTGAACCTGGACGAGAGGGAAGCCCCGGTGCTGATGGCCCCCCTGGCAGAGATGGC GCTGCTGGAGTCAAGGGTGATCGTGGTGAGACTGGTGCTGTGGGAGCTCCTGGAGCCCCTGGGCCCCCTGGCTCCCCTGGCCCCGCTGGTCCAACTGGCA AGCAAGGAGACAGAGGAGAAGCTGGTGCACAAGGCCCCATGGGACCCTCAGGACCAGCTGGAGCCCGGGGAATCCAGGGTCCTCAAGGCCCCAGAGGTGA CAAAGGAGAGGCTGGAGAGCCTGGCGAGAGAGGCCTGAAGGGACACCGTGGCTTCACTGGTCTGCAGGGTCTGCCCGGCCCTCCTGGTCCTTCTGGAGAC CAAGGTGCTTCTGGTCCTGCTGGTCCTTCTGGCCCTAGAGGTCCTCCTGGCCCCGTCGGTCCCTCTGGCAAAGATGGTGCTAATGGAATCCCTGGCCCCA TTGGGCCTCCTGGTCCCCGTGGACGATCAGGCGAAACCGGCCCTGCTGGTCCTCCTGGAAATCCTGGACCCCCTGGTCCTCCAGGTCCCCCTGGCCCTGG CATCGACATGTCCGCCTTTGCTGGCTTAGGCCCGAGAGAGAAGGGCCCCGACCCCCTGCAGTACATGCGGGCCGACCAGGCAGCCGGTGGCCTGAGACAG CATGACGCCGAGGTGGATGCCACACTCAAGTCCCTCAACAACCAGATTGAGAGCATCCGCAGCCCCGAGGGCTCCCGCAAGAACCCTGCTCGCACCTGCA GAGACCTGAAACTCTGCCACCCTGAGTGGAAGAGTGGAGACTACTGGATTGACCCCAACCAAGGCTGCACCTTGGACGCCATGAAGGTTTTCTGCAACAT GGAGACTGGCGAGACTTGCGTCTACCCCAATCCAGCAAACGTTCCCAAGAAGAACTGGTGGAGCAGCAAGAGCAAGGAGAAGAAACACATCTGGTTTGGA GAAACCATCAATGGTGGCTTCCATTTCAGCTATGGAGATGACAATCTGGCTCCCAACACTGCCAACGTCCAGATGACCTTCCTACGCCTGCTGTCCACGG AAGGCTCCCAGAACATCACCTACCACTGCAAGAACAGCATTGCCTATCTGGACGAAGCAGCTGGCAACCTCAAGAAGGCCCTGCTCATCCAGGGCTCCAA TGACGTGGAGATCCGGGCAGAGGGCAATAGCAGGTTCACGTACACTGCCCTGAAGGATGGCTGCACGAAACATACCGGTAAGTGGGGCAAGACTGTTATC GAGTACCGGTCACAGAAGACCTCACGCCTCCCCATCATTGACATTGCACCCATGGACATAGGAGGGCCCGAGCAGGAATTCGGTGTGGACATAGGGCCGG TCTGCTTCTTGTAAAAACCTGAACCCAGAAACAACACAATCCGTTGCAAACCCAAAGGACCCAAGTACTTTCCAATCTCAGTCACTCTAGGACTCTGCAC TGAATGGCTGACCTGACCTGATGTCCATTCATCCCACCCTCTCACAGTTCGGACTTTTCTCCCCTCTCTTTCTAAGAGACCTGAACTGGGCAGACTGCAA AATAAAATCTCGGTGTTCTATTTATTTATTGTCTTCCTGTAAGACCTTCGGGTCAAGGCAGAGGCAGGAAACTAACTGGTGTGAGTCAAATGCCCCCTGA GTGACTGCCCCCAGCCCAGGCCAGAAGACCTCCCTTCAGGTGCCGGGCGCAGGAACTGTGTGTGTCCTACACAATGGTGCTATTCTGTGTCAAACACCTC TGTATTTTTTAAAACATCAATTGATATTAAAAATGAAAAGATTATTGGAAAGT

refseq_COL2A1.F1 (forward primer)
refseq_COL2A1.R1 (reverse primer)
Single exon skipping

Frame 0

TQSAAGLPGLPLPPPAPRASCMRAR*RPGPAPC SCRFAALRPGPAQPGPAVSHDSPRGSPDAGAADA ARRRCPSVSGPGCPATRTKGTERRTWRHQGYCR TQRTSWASGTCRGTRTQRGSW*QR*KRCPWTSW QRWRTWDPWKSWPPWSSRPPWSPWSWWKLCCPDG WRI**KGWWRPVGSNARTNGPHGTSRTSRPCRC SWASRISRQSW*TW*TWCLWSHGSPWSSWSPWK AW**W*SWKTWKSW*KGSAWSSGCSWFPRNPRPS WCQRSQRLSRPGRC*GRGGCSWCEG*EWFPG*E RISGPNGSSWPAW*KRTDWPCWRCGCPRQRWSA RPRRASGSCRSCWWSWLPWCSWSQG*SRPHWCPW S*RCSRSSR*TWYSWVPWACWCLR*PWNRWNSW SQRICWCSWHCWCSWLPWATGPSWPSRCNWSSG PERSDG*TWYCWLQR*TRPQGRTWPCWPPGSPWT RW*RRQERCPWRAWWRWAHRSPWRKRCSRQPRF PRSRWSGRSQGSPWRARAQWSCWPQGSQR*PWP SWRTWPSWSPGSHWPPW*CWSSRQSWPFWSPW*R WSSWTSRSSGGSWAAWCHGFPWPQRCQR*AWQS W*EGTAWCSWSEGSSWQRW*DRCCRTPWPCWTC W*TRRAGCSWAIWVPGTSWPSWSPR*RWKTR*PG CSR*SWSPWPRGSQG*TRFPR*TWLSRCPGPPG SPWPPRHSWH*WSQRCIWPSRPPWGSGPSRSSG NAWREGSSWYRWAQRRQG*RW*ERP*GSPWKGWW TRPDRSHWPPWPSWC*WREGRSWTSWSCRKCWC SWRSG*TWRDWAPRTSGICWASWC*WPAWGQG* ARRGRPERRCWCPWSSGPLWSTWASGSYWSDWS* RSPRCPRPPGSHWIPWSCWPRWTPRLQWQPWTP WSPWSFWKRWSQRCSRRQRPPWPSW*TRPPRSC WTPWREGRAWR*RSLWCRRSTRSPGSGWSERHRR SAWATW*ERIPWLAWPVG*ARQAGCSWSIWRQR SSWPRGSSWPDGSCR*TWTRGKPRC*WPPWQRW RCWSQG*SW*DWCCGSSWSPWAPWLPWPRWSNWQ ARRQRRSWCTRPHGTLRTSWSPGNPGSSRPQR* QRRGWRAWRERPEGTPWLHWSAGSARPSWSFWR PRCFWSCWSFWP*RSSWPRRSLWQRWC*WNPWPH WASWSPWTIRRNRPCWSSWKSWTPWSSRSPWPW HRHVRLCWLRPEREGPRPPAVHAGRPGSRWPET A*RRGGCHTQVPQQPD*EHPQPRGLPQEPCSHLQ RPETLPP*VEEWRLLD*PQPRLHLGRHEGFLQH GDWRDLRLPQSSKRSQEELVEQQEQGEETHLVW RNHQWWLPFQLWR*QSGSQHCQRPDDLPTPAVHG RLPEHHLPLQEQHCLSGRSSWQPQEGPAHPGLQ *RGDPGRGQ*QVHVHCPEGWLHETYR*VGQDCY RVPVTEDLTPPHH*HCTHGHRRARAGIRCGHRAG LLLVKT*TQKQHNPLQTQRTQVLSNLSHSRTLH *MADLT*CPFIPPSHSSDFSPLSF*ET*TGQTA K*NLGVLFIYCLPVRPSGQGRGRKLTGVSQMPPE *LPPAQARRPPFRCRAQELCVSYTMVLFCVKHL CIF*NIN*Y*K*KDYWK

Frame 1

RRALLGCRVSRFPLLLQGPPA*GRGRDPDPRRA PAVSLRSARARLSQAPR*AMIRLGAPQTLVLLT LLVAAVLRCQGQDVRQPGPKGQKGEPGDIKDIVG PKGPPGPQGPAGEQGPRGDRGDKGEKGAPGPRG RDGEPGTPGNPGPPGPPGPPGPPGLGGNFAAQM AGGFDEKAGGAQLGVMQGPMGPMGPRGPPGPAGA PGPQGFQGNPGEPGEPGVSGPMGPRGPPGPPGK PGDDGEAGKPGKAGERGPPGPQGARGFPGTPGL PGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGEN GSPGPMGPRGLPGERGRTGPAGAAGARGNDGQP GPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGAR GPEGAQGPRGEPGTPGSPGPAGASGNPGTDGIPG AKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLG PKGQTGEPGIAGFKGEQGPKGEPGPAGPQGAPG PAGEEGKRGARGEPGGVGPIGPPGERGAPGNRGF PGQDGLAGPKGAPGERGPSGLAGPKGANGDPGR PGEPGLPGARGLTGRPGDAGPQGKVGPSGAPGE DGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKA GEKGLPGAPGLRGLPGKDGETGAAGPPGPAGPA GERGEQGAPGPSGFQGLPGPPGPPGEGGKPGDQ GVPGEAGAPGLVGPRGERGFPGERGSPGAQGLQG PRGLPGTPGTDGPKGASGPAGPPGAQGPPGLQG MPGERGAAGIAGPKGDRGDVGEKGPEGAPGKDG GRGLTGPIGPPGPAGANGEKGEVGPPGPAGSAGA RGAPGERGETGPPGPAGFAGPPGADGQPGAKGE QGEAGQKGDAGAPGPQGPSGAPGPQGPTGVTGP KGARGAQGPPGATGFPGAAGRVGPPGSNGNPGPP GPPGPSGKDGPKGARGDSGPPGRAGEPGLQGPA GPPGEKGEPGDDGPSGAEGPPGPQGLAGQRGIV GLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRG PPGPVGPPGLTGPAGEPGREGSPGADGPPGRDG AAGVKGDRGETGAVGAPGAPGPPGSPGPAGPTG KQGDRGEAGAQGPMGPSGPAGARGIQGPQGPRGD KGEAGEPGERGLKGHRGFTGLQGLPGPPGPSGD QGASGPAGPSGPRGPPGPVGPSGKDGANGIPGP IGPPGPRGRSGETGPAGPPGNPGPPGPPGPPGPG IDMSAFAGLGPREKGPDPLQYMRADQAAGGLRQ HDAEVDATLKSLNNQIESIRSPEGSRKNPARTC RDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNM ETGETCVYPNPANVPKKNWWSSKSKEKKHIWFG ETINGGFHFSYGDDNLAPNTANVQMTFLRLLST EGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSN DVEIRAEGNSRFTYTALKDGCTKHTGKWGKTVI EYRSQKTSRLPIIDIAPMDIGGPEQEFGVDIGP VCFL*KPEPRNNTIRCKPKGPKYFPISVTLGLCT EWLT*PDVHSSHPLTVRTFLPSLSKRPELGRLQ NKISVFYLFIVFL*DLRVKAEAGN*LV*VKCPL SDCPQPRPEDLPSGAGRRNCVCPTQWCYSVSNTS VFFKTSIDIKNEKIIGK

Frame 2

AERCWAAGSPASPSCSKGLLHEGAVETRTRAVL LPFRCAPPGPGSARPRGEP*FASGLPRRWCC*R CSSPLSFGVRARMSGNQDQRDRKENLETSRIL* DPKDLLGLRDLQGNKDPEGIVVTKVKKVPLDLVA EMENLGPLEILAPLVLPAPLVPLVLVETLLPRW LEDLMKRLVAPSWE*CKDQWAPWDLEDLQALQV LLGLKDFKAILVNLVNLVSLVPWVPVVLLVPLES LVMMVKLENLEKLVKGVRLVLRVLVVSQEPQAF LVSKVTEVIQAWTVLRERRVLLV*RVRVVPRVR TDLRAQWVLVACLVKEDGLALLALRVPEATMVSQ APQGLRVLSVLLVVLASLVLLEPRVKPAPLVPV VLKVLKVLAVNLVLLGPLGLLVPPVTLEQMEFL EPKDLLVLLALLVLLASLGHGALLALKVQLVLWA RKVRRVNLVLLASKVNKAPRENLALLAPREPLD PLVKKAREVPVESLVALGPSVPLEKEVLPATAV SQVKMVWQVPREPLESEGPVVLLAPREPTVTLAV LENLAFLEPGVSLAALVMLVLKAKLALLEPLVK MVVLDLQVLRGLVGSLVSWVSLAPKVPTVSLAK LVRRDCLVLLV*GVFLAKMVRQVLQDPLALLDLL VNEASRVLLGHLGSRDFLALLVPQVKVENQVTR VFPVKLEPLASWVPGVNEVSQVNVALPVPRASR VPVASPALLALMVPKVHLAQQAPLGLRALQVFRE CLARGEQLVSLGPKATGVTLVRKALREPLERMV DEA*QVPLAPLAQLVLMARREKLDLLVLQEVLV LVALRVNVERLGPPDQRDLLGLLVLMASLGPRVS KERPARKAMLVPLVLRAPLEHLGLRVLLE*LVL KEPEVPKAPREPLDSLELLAALDPQAPMATLDP LVPLVLLEKMVPKVLEETAAPLAELVNPASKVLL DPLARRESLEMTVPLVPKVHQVPRVWLVREASS VCLGNVVREDSLACLARRVSPASRVLLEHLETE VLLAPWVLLA*RVLQVNLDEREAPVLMAPLAEMA LLESRVIVVRLVLWELLEPLGPLAPLAPLVQLA SKETEEKLVHKAPWDPQDQLEPGESRVLKAPEV TKERLESLAREA*RDTVASLVCRVCPALLVLLET KVLLVLLVLLALEVLLAPSVPLAKMVLMESLAP LGLLVPVDDQAKPALLVLLEILDPLVLQVPLAL ASTCPPLLA*ARERRAPTPCSTCGPTRQPVA*DS MTPRWMPHSSPSTTRLRASAAPRAPARTLLAPA ET*NSATLSGRVETTGLTPTKAAPWTP*RFSAT WRLARLASTPIQQTFPRRTGGAARARRRNTSGLE KPSMVASISAMEMTIWLPTLPTSR*PSYACCPR KAPRTSPTTARTALPIWTKQLATSRRPCSSRAP MTWRSGQRAIAGSRTLP*RMAARNIPVSGARLLS STGHRRPHASPSLTLHPWT*EGPSRNSVWT*GR SASCKNLNPETTQSVANPKDPSTFQSQSL*DSA LNG*PDLMSIHPTLSQFGLFSPLFLRDLNWADCK IKSRCSIYLLSSCKTFGSRQRQETNWCESNAP* VTAPSPGQKTSLQVPGAGTVCVLHNGAILCQTP LYFLKHQLILKMKRLLES

Sequence NM_001844

ACGCAGAGCGCTGCTGGGCTGCCGGGTCTCCCGCTTCCCCCTCCTGCTCCAAGGGCCTCCTGCATGAGGGCGCGGTAGAGACCCGGACCCGCGCCGTGCT CCTGCCGTTTCGCTGCGCTCCGCCCGGGCCCGGCTCAGCCAGGCCCCGCGGTGAGCCATGATTCGCCTCGGGGCTCCCCAGACGCTGGTGCTGCTGACGC TGCTCGTCGCCGCTGTCCTTCGGTGTCAGGGCCAGGATGTCCAGGAGGCTGGCAGCTGTGTGCAGGATGGGCAGAGGTATAATGATAAGGATGTGTGGAA GCCGGAGCCCTGCCGGATCTGTGTCTGTGACACTGGGACTGTCCTCTGCGACGACATAATCTGTGAAGACGTGAAAGACTGCCTCAGCCCTGAGATCCCC TTCGGAGAGTGCTGCCCCATCTGCCCAACTGACCTCGCCACTGCCAGTGGGCAACCAGGACCAAAGGGACAGAAAGGAGAACCTGGAGACATCAAGGATA TTGTAGGACCCAAAGGACCTCCTGGGCCTCAGGGACCTGCAGGGGAACAAGGACCCAGAGGGGATCGTGGTGACAAAGGTGAAAAAGGTGCCCCTGGACC TCGTGGCAGAGATGGAGAACCTGGGACCCCTGGAAATCCTGGCCCCCCTGGTCCTCCCGGCCCCCCTGGTCCCCCTGGTCTTGGTGGAAACTTTGCTGCC CAGATGGCTGGAGGATTTGATGAAAAGGCTGGTGGCGCCCAGTTGGGAGTAATGCAAGGACCAATGGGCCCCATGGGACCTCGAGGACCTCCAGGCCCTG CAGGTGCTCCTGGGCCTCAAGGATTTCAAGGCAATCCTGGTGAACCTGGTGAACCTGGTGTCTCTGGTCCCATGGGTCCCCGTGGTCCTCCTGGTCCCCC TGGAAAGCCTGGTGATGATGGTGAAGCTGGAAAACCTGGAAAAGCTGGTGAAAGGGGTCCGCCTGGTCCTCAGGGTGCTCGTGGTTTCCCAGGAACCCCA GGCCTTCCTGGTGTCAAAGGTCACAGAGGTTATCCAGGCCTGGACGGTGCTAAGGGAGAGGCGGGTGCTCCTGGTGTGAAGGGTGAGAGTGGTTCCCCGG GTGAGAACGGATCTCCGGGCCCAATGGGTCCTCGTGGCCTGCCTGGTGAAAGAGGACGGACTGGCCCTGCTGGCGCTGCGGGTGCCCGAGGCAACGATGG TCAGCCAGGCCCCGCAGGGCCTCCGGGTCCTGTCGGTCCTGCTGGTGGTCCTGGCTTCCCTGGTGCTCCTGGAGCCAAGGGTGAAGCCGGCCCCACTGGT GCCCGTGGTCCTGAAGGTGCTCAAGGTCCTCGCGGTGAACCTGGTACTCCTGGGTCCCCTGGGCCTGCTGGTGCCTCCGGTAACCCTGGAACAGATGGAA TTCCTGGAGCCAAAGGATCTGCTGGTGCTCCTGGCATTGCTGGTGCTCCTGGCTTCCCTGGGCCACGGGGCCCTCCTGGCCCTCAAGGTGCAACTGGTCC TCTGGGCCCGAAAGGTCAGACGGGTGAACCTGGTATTGCTGGCTTCAAAGGTGAACAAGGCCCCAAGGGAGAACCTGGCCCTGCTGGCCCCCAGGGAGCC CCTGGACCCGCTGGTGAAGAAGGCAAGAGAGGTGCCCGTGGAGAGCCTGGTGGCGTTGGGCCCATCGGTCCCCCTGGAGAAAGAGGTGCTCCCGGCAACC GCGGTTTCCCAGGTCAAGATGGTCTGGCAGGTCCCAAGGGAGCCCCTGGAGAGCGAGGGCCCAGTGGTCTTGCTGGCCCCAAGGGAGCCAACGGTGACCC TGGCCGTCCTGGAGAACCTGGCCTTCCTGGAGCCCGGGGTCTCACTGGCCGCCCTGGTGATGCTGGTCCTCAAGGCAAAGTTGGCCCTTCTGGAGCCCCT GGTGAAGATGGTCGTCCTGGACCTCCAGGTCCTCAGGGGGCTCGTGGGCAGCCTGGTGTCATGGGTTTCCCTGGCCCCAAAGGTGCCAACGGTGAGCCTG GCAAAGCTGGTGAGAAGGGACTGCCTGGTGCTCCTGGTCTGAGGGGTCTTCCTGGCAAAGATGGTGAGACAGGTGCTGCAGGACCCCCTGGCCCTGCTGG ACCTGCTGGTGAACGAGGCGAGCAGGGTGCTCCTGGGCCATCTGGGTTCCAGGGACTTCCTGGCCCTCCTGGTCCCCCAGGTGAAGGTGGAAAACCAGGT GACCAGGGTGTTCCCGGTGAAGCTGGAGCCCCTGGCCTCGTGGGTCCCAGGGGTGAACGAGGTTTCCCAGGTGAACGTGGCTCTCCCGGTGCCCAGGGCC TCCAGGGTCCCCGTGGCCTCCCCGGCACTCCTGGCACTGATGGTCCCAAAGGTGCATCTGGCCCAGCAGGCCCCCCTGGGGCTCAGGGCCCTCCAGGTCT TCAGGGAATGCCTGGCGAGAGGGGAGCAGCTGGTATCGCTGGGCCCAAAGGCGACAGGGGTGACGTTGGTGAGAAAGGCCCTGAGGGAGCCCCTGGAAAG GATGGTGGACGAGGCCTGACAGGTCCCATTGGCCCCCCTGGCCCAGCTGGTGCTAATGGCGAGAAGGGAGAAGTTGGACCTCCTGGTCCTGCAGGAAGTG CTGGTGCTCGTGGCGCTCCGGGTGAACGTGGAGAGACTGGGCCCCCCGGACCAGCGGGATTTGCTGGGCCTCCTGGTGCTGATGGCCAGCCTGGGGCCAA GGGTGAGCAAGGAGAGGCCGGCCAGAAAGGCGATGCTGGTGCCCCTGGTCCTCAGGGCCCCTCTGGAGCACCTGGGCCTCAGGGTCCTACTGGAGTGACT GGTCCTAAAGGAGCCCGAGGTGCCCAAGGCCCCCCGGGAGCCACTGGATTCCCTGGAGCTGCTGGCCGCGTTGGACCCCCAGGCTCCAATGGCAACCCTG GACCCCCTGGTCCCCCTGGTCCTTCTGGAAAAGATGGTCCCAAAGGTGCTCGAGGAGACAGCGGCCCCCCTGGCCGAGCTGGTGAACCCGGCCTCCAAGG TCCTGCTGGACCCCCTGGCGAGAAGGGAGAGCCTGGAGATGACGGTCCCTCTGGTGCCGAAGGTCCACCAGGTCCCCAGGGTCTGGCTGGTCAGAGAGGC ATCGTCGGTCTGCCTGGGCAACGTGGTGAGAGAGGATTCCCTGGCTTGCCTGGCCCGTCGGGTGAGCCCGGCAAGCAGGGTGCTCCTGGAGCATCTGGAG ACAGAGGTCCTCCTGGCCCCGTGGGTCCTCCTGGCCTGACGGGTCCTGCAGGTGAACCTGGACGAGAGGGAAGCCCCGGTGCTGATGGCCCCCCTGGCAG AGATGGCGCTGCTGGAGTCAAGGGTGATCGTGGTGAGACTGGTGCTGTGGGAGCTCCTGGAGCCCCTGGGCCCCCTGGCTCCCCTGGCCCCGCTGGTCCA ACTGGCAAGCAAGGAGACAGAGGAGAAGCTGGTGCACAAGGCCCCATGGGACCCTCAGGACCAGCTGGAGCCCGGGGAATCCAGGGTCCTCAAGGCCCCA GAGGTGACAAAGGAGAGGCTGGAGAGCCTGGCGAGAGAGGCCTGAAGGGACACCGTGGCTTCACTGGTCTGCAGGGTCTGCCCGGCCCTCCTGGTCCTTC TGGAGACCAAGGTGCTTCTGGTCCTGCTGGTCCTTCTGGCCCTAGAGGTCCTCCTGGCCCCGTCGGTCCCTCTGGCAAAGATGGTGCTAATGGAATCCCT GGCCCCATTGGGCCTCCTGGTCCCCGTGGACGATCAGGCGAAACCGGCCCTGCTGGTCCTCCTGGAAATCCTGGACCCCCTGGTCCTCCAGGTCCCCCTG GCCCTGGCATCGACATGTCCGCCTTTGCTGGCTTAGGCCCGAGAGAGAAGGGCCCCGACCCCCTGCAGTACATGCGGGCCGACCAGGCAGCCGGTGGCCT GAGACAGCATGACGCCGAGGTGGATGCCACACTCAAGTCCCTCAACAACCAGATTGAGAGCATCCGCAGCCCCGAGGGCTCCCGCAAGAACCCTGCTCGC ACCTGCAGAGACCTGAAACTCTGCCACCCTGAGTGGAAGAGTGGAGACTACTGGATTGACCCCAACCAAGGCTGCACCTTGGACGCCATGAAGGTTTTCT GCAACATGGAGACTGGCGAGACTTGCGTCTACCCCAATCCAGCAAACGTTCCCAAGAAGAACTGGTGGAGCAGCAAGAGCAAGGAGAAGAAACACATCTG GTTTGGAGAAACCATCAATGGTGGCTTCCATTTCAGCTATGGAGATGACAATCTGGCTCCCAACACTGCCAACGTCCAGATGACCTTCCTACGCCTGCTG TCCACGGAAGGCTCCCAGAACATCACCTACCACTGCAAGAACAGCATTGCCTATCTGGACGAAGCAGCTGGCAACCTCAAGAAGGCCCTGCTCATCCAGG GCTCCAATGACGTGGAGATCCGGGCAGAGGGCAATAGCAGGTTCACGTACACTGCCCTGAAGGATGGCTGCACGAAACATACCGGTAAGTGGGGCAAGAC TGTTATCGAGTACCGGTCACAGAAGACCTCACGCCTCCCCATCATTGACATTGCACCCATGGACATAGGAGGGCCCGAGCAGGAATTCGGTGTGGACATA GGGCCGGTCTGCTTCTTGTAAAAACCTGAACCCAGAAACAACACAATCCGTTGCAAACCCAAAGGACCCAAGTACTTTCCAATCTCAGTCACTCTAGGAC TCTGCACTGAATGGCTGACCTGACCTGATGTCCATTCATCCCACCCTCTCACAGTTCGGACTTTTCTCCCCTCTCTTTCTAAGAGACCTGAACTGGGCAG ACTGCAAAATAAAATCTCGGTGTTCTATTTATTTATTGTCTTCCTGTAAGACCTTCGGGTCAAGGCAGAGGCAGGAAACTAACTGGTGTGAGTCAAATGC CCCCTGAGTGACTGCCCCCAGCCCAGGCCAGAAGACCTCCCTTCAGGTGCCGGGCGCAGGAACTGTGTGTGTCCTACACAATGGTGCTATTCTGTGTCAA ACACCTCTGTATTTTTTAAAACATCAATTGATATTAAAAATGAAAAGATTATTGGAAAGT

refseq_COL2A1.F1 (forward primer)
refseq_COL2A1.R1 (reverse primer)
Single exon skipping

Frame 0

TQSAAGLPGLPLPPPAPRASCMRAR*RPGPAPC SCRFAALRPGPAQPGPAVSHDSPRGSPDAGAADA ARRRCPSVSGPGCPGGWQLCAGWAEV***GCVE AGALPDLCL*HWDCPLRRHNL*RRERLPQP*DP LRRVLPHLPN*PRHCQWATRTKGTERRTWRHQGY CRTQRTSWASGTCRGTRTQRGSW*QR*KRCPWT SWQRWRTWDPWKSWPPWSSRPPWSPWSWWKLCC PDGWRI**KGWWRPVGSNARTNGPHGTSRTSRPC RCSWASRISRQSW*TW*TWCLWSHGSPWSSWSP WKAW**W*SWKTWKSW*KGSAWSSGCSWFPRNP RPSWCQRSQRLSRPGRC*GRGGCSWCEG*EWFPG *ERISGPNGSSWPAW*KRTDWPCWRCGCPRQRW SARPRRASGSCRSCWWSWLPWCSWSQG*SRPHW CPWS*RCSRSSR*TWYSWVPWACWCLR*PWNRWN SWSQRICWCSWHCWCSWLPWATGPSWPSRCNWS SGPERSDG*TWYCWLQR*TRPQGRTWPCWPPGS PWTRW*RRQERCPWRAWWRWAHRSPWRKRCSRQP RFPRSRWSGRSQGSPWRARAQWSCWPQGSQR*P WPSWRTWPSWSPGSHWPPW*CWSSRQSWPFWSP W*RWSSWTSRSSGGSWAAWCHGFPWPQRCQR*AW QSW*EGTAWCSWSEGSSWQRW*DRCCRTPWPCW TCW*TRRAGCSWAIWVPGTSWPSWSPR*RWKTR *PGCSR*SWSPWPRGSQG*TRFPR*TWLSRCPGP PGSPWPPRHSWH*WSQRCIWPSRPPWGSGPSRS SGNAWREGSSWYRWAQRRQG*RW*ERP*GSPWK GWWTRPDRSHWPPWPSWC*WREGRSWTSWSCRKC WCSWRSG*TWRDWAPRTSGICWASWC*WPAWGQ G*ARRGRPERRCWCPWSSGPLWSTWASGSYWSD WS*RSPRCPRPPGSHWIPWSCWPRWTPRLQWQPW TPWSPWSFWKRWSQRCSRRQRPPWPSW*TRPPR SCWTPWREGRAWR*RSLWCRRSTRSPGSGWSER HRRSAWATW*ERIPWLAWPVG*ARQAGCSWSIWR QRSSWPRGSSWPDGSCR*TWTRGKPRC*WPPWQ RWRCWSQG*SW*DWCCGSSWSPWAPWLPWPRWS NWQARRQRRSWCTRPHGTLRTSWSPGNPGSSRPQ R*QRRGWRAWRERPEGTPWLHWSAGSARPSWSF WRPRCFWSCWSFWP*RSSWPRRSLWQRWC*WNP WPHWASWSPWTIRRNRPCWSSWKSWTPWSSRSPW PWHRHVRLCWLRPEREGPRPPAVHAGRPGSRWP ETA*RRGGCHTQVPQQPD*EHPQPRGLPQEPCS HLQRPETLPP*VEEWRLLD*PQPRLHLGRHEGFL QHGDWRDLRLPQSSKRSQEELVEQQEQGEETHL VWRNHQWWLPFQLWR*QSGSQHCQRPDDLPTPA VHGRLPEHHLPLQEQHCLSGRSSWQPQEGPAHPG LQ*RGDPGRGQ*QVHVHCPEGWLHETYR*VGQD CYRVPVTEDLTPPHH*HCTHGHRRARAGIRCGH RAGLLLVKT*TQKQHNPLQTQRTQVLSNLSHSRT LH*MADLT*CPFIPPSHSSDFSPLSF*ET*TGQ TAK*NLGVLFIYCLPVRPSGQGRGRKLTGVSQM PPE*LPPAQARRPPFRCRAQELCVSYTMVLFCVK HLCIF*NIN*Y*K*KDYWK

Frame 1

RRALLGCRVSRFPLLLQGPPA*GRGRDPDPRRA PAVSLRSARARLSQAPR*AMIRLGAPQTLVLLT LLVAAVLRCQGQDVQEAGSCVQDGQRYNDKDVWK PEPCRICVCDTGTVLCDDIICEDVKDCLSPEIP FGECCPICPTDLATASGQPGPKGQKGEPGDIKD IVGPKGPPGPQGPAGEQGPRGDRGDKGEKGAPGP RGRDGEPGTPGNPGPPGPPGPPGPPGLGGNFAA QMAGGFDEKAGGAQLGVMQGPMGPMGPRGPPGP AGAPGPQGFQGNPGEPGEPGVSGPMGPRGPPGPP GKPGDDGEAGKPGKAGERGPPGPQGARGFPGTP GLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSP GENGSPGPMGPRGLPGERGRTGPAGAAGARGNDG QPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTG ARGPEGAQGPRGEPGTPGSPGPAGASGNPGTDG IPGAKGSAGAPGIAGAPGFPGPRGPPGPQGATGP LGPKGQTGEPGIAGFKGEQGPKGEPGPAGPQGA PGPAGEEGKRGARGEPGGVGPIGPPGERGAPGN RGFPGQDGLAGPKGAPGERGPSGLAGPKGANGDP GRPGEPGLPGARGLTGRPGDAGPQGKVGPSGAP GEDGRPGPPGPQGARGQPGVMGFPGPKGANGEP GKAGEKGLPGAPGLRGLPGKDGETGAAGPPGPAG PAGERGEQGAPGPSGFQGLPGPPGPPGEGGKPG DQGVPGEAGAPGLVGPRGERGFPGERGSPGAQG LQGPRGLPGTPGTDGPKGASGPAGPPGAQGPPGL QGMPGERGAAGIAGPKGDRGDVGEKGPEGAPGK DGGRGLTGPIGPPGPAGANGEKGEVGPPGPAGS AGARGAPGERGETGPPGPAGFAGPPGADGQPGAK GEQGEAGQKGDAGAPGPQGPSGAPGPQGPTGVT GPKGARGAQGPPGATGFPGAAGRVGPPGSNGNP GPPGPPGPSGKDGPKGARGDSGPPGRAGEPGLQG PAGPPGEKGEPGDDGPSGAEGPPGPQGLAGQRG IVGLPGQRGERGFPGLPGPSGEPGKQGAPGASG DRGPPGPVGPPGLTGPAGEPGREGSPGADGPPGR DGAAGVKGDRGETGAVGAPGAPGPPGSPGPAGP TGKQGDRGEAGAQGPMGPSGPAGARGIQGPQGP RGDKGEAGEPGERGLKGHRGFTGLQGLPGPPGPS GDQGASGPAGPSGPRGPPGPVGPSGKDGANGIP GPIGPPGPRGRSGETGPAGPPGNPGPPGPPGPP GPGIDMSAFAGLGPREKGPDPLQYMRADQAAGGL RQHDAEVDATLKSLNNQIESIRSPEGSRKNPAR TCRDLKLCHPEWKSGDYWIDPNQGCTLDAMKVF CNMETGETCVYPNPANVPKKNWWSSKSKEKKHIW FGETINGGFHFSYGDDNLAPNTANVQMTFLRLL STEGSQNITYHCKNSIAYLDEAAGNLKKALLIQ GSNDVEIRAEGNSRFTYTALKDGCTKHTGKWGKT VIEYRSQKTSRLPIIDIAPMDIGGPEQEFGVDI GPVCFL*KPEPRNNTIRCKPKGPKYFPISVTLG LCTEWLT*PDVHSSHPLTVRTFLPSLSKRPELGR LQNKISVFYLFIVFL*DLRVKAEAGN*LV*VKC PLSDCPQPRPEDLPSGAGRRNCVCPTQWCYSVS NTSVFFKTSIDIKNEKIIGK

Frame 2

AERCWAAGSPASPSCSKGLLHEGAVETRTRAVL LPFRCAPPGPGSARPRGEP*FASGLPRRWCC*R CSSPLSFGVRARMSRRLAAVCRMGRGIMIRMCG SRSPAGSVSVTLGLSSATT*SVKT*KTASALRSP SESAAPSAQLTSPLPVGNQDQRDRKENLETSRI L*DPKDLLGLRDLQGNKDPEGIVVTKVKKVPLD LVAEMENLGPLEILAPLVLPAPLVPLVLVETLLP RWLEDLMKRLVAPSWE*CKDQWAPWDLEDLQAL QVLLGLKDFKAILVNLVNLVSLVPWVPVVLLVP LESLVMMVKLENLEKLVKGVRLVLRVLVVSQEPQ AFLVSKVTEVIQAWTVLRERRVLLV*RVRVVPR VRTDLRAQWVLVACLVKEDGLALLALRVPEATM VSQAPQGLRVLSVLLVVLASLVLLEPRVKPAPLV PVVLKVLKVLAVNLVLLGPLGLLVPPVTLEQME FLEPKDLLVLLALLVLLASLGHGALLALKVQLV LWARKVRRVNLVLLASKVNKAPRENLALLAPREP LDPLVKKAREVPVESLVALGPSVPLEKEVLPAT AVSQVKMVWQVPREPLESEGPVVLLAPREPTVT LAVLENLAFLEPGVSLAALVMLVLKAKLALLEPL VKMVVLDLQVLRGLVGSLVSWVSLAPKVPTVSL AKLVRRDCLVLLV*GVFLAKMVRQVLQDPLALL DLLVNEASRVLLGHLGSRDFLALLVPQVKVENQV TRVFPVKLEPLASWVPGVNEVSQVNVALPVPRA SRVPVASPALLALMVPKVHLAQQAPLGLRALQV FRECLARGEQLVSLGPKATGVTLVRKALREPLER MVDEA*QVPLAPLAQLVLMARREKLDLLVLQEV LVLVALRVNVERLGPPDQRDLLGLLVLMASLGP RVSKERPARKAMLVPLVLRAPLEHLGLRVLLE*L VLKEPEVPKAPREPLDSLELLAALDPQAPMATL DPLVPLVLLEKMVPKVLEETAAPLAELVNPASK VLLDPLARRESLEMTVPLVPKVHQVPRVWLVREA SSVCLGNVVREDSLACLARRVSPASRVLLEHLE TEVLLAPWVLLA*RVLQVNLDEREAPVLMAPLA EMALLESRVIVVRLVLWELLEPLGPLAPLAPLVQ LASKETEEKLVHKAPWDPQDQLEPGESRVLKAP EVTKERLESLAREA*RDTVASLVCRVCPALLVL LETKVLLVLLVLLALEVLLAPSVPLAKMVLMESL APLGLLVPVDDQAKPALLVLLEILDPLVLQVPL ALASTCPPLLA*ARERRAPTPCSTCGPTRQPVA *DSMTPRWMPHSSPSTTRLRASAAPRAPARTLLA PAET*NSATLSGRVETTGLTPTKAAPWTP*RFS ATWRLARLASTPIQQTFPRRTGGAARARRRNTS GLEKPSMVASISAMEMTIWLPTLPTSR*PSYACC PRKAPRTSPTTARTALPIWTKQLATSRRPCSSR APMTWRSGQRAIAGSRTLP*RMAARNIPVSGAR LLSSTGHRRPHASPSLTLHPWT*EGPSRNSVWT* GRSASCKNLNPETTQSVANPKDPSTFQSQSL*D SALNG*PDLMSIHPTLSQFGLFSPLFLRDLNWA DCKIKSRCSIYLLSSCKTFGSRQRQETNWCESNA P*VTAPSPGQKTSLQVPGAGTVCVLHNGAILCQ TPLYFLKHQLILKMKRLLES