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Sequences

Sequence NM_080815

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGG CCACCCAGGACCAAAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCA CACCGGGAGTGCCTAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAG GGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGG AGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTG GCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGA AAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGG CCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGG AAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTG GCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGG AGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGG GAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAA AGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGTACTCCAGGACCAAT TGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACAT GGACCACCCGGGGACAAGGGAAACCGGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGT GTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTG CATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCT GCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCA TGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAAT GAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ HFRVPLEDPDSQVNQELEVSLDFRVPLGWTANR ATQDQRATWV*RVPQDSRDPRDKKEKRVSVESTH TGSA*AACQQLCAPAK*LP*RANRARPASKVHQ GPQAPLDQVDLWGTQDCQGLWGHLAYLGLLDQR ETQGSRATTAGRENGACQGCQASMEPRGRPELPW LG*RVSQGSQEPRVRRGLKAPLGFLASWGRRER KAMLATPLEEAEGNLALQGSLGPQGQREKQVSM ARLAPQGSQETRGSVEQLENRDQMAPRAPRENQG KERWWITMETSMRLSRRSGRWP*WGLLVFLGKL AHLELQGFQARRGRLDCQALQDTMGKRDLAVNQ ETWALLVPKAPQERMDLQE*REKTGTQGAQERRG KKGRQDKQAHRFLGCQGQRGLPDLRGSKVFLDQ RGKQD*MEQKERKASREKKETVVPWDYPVLQDQ LEFQAQRDQRARGAAKETLG*QDQREQLGFLVYM DHPGTRETGAKMAYQSKAAGTSDASNLGLACVC VCT*NIYFYTVFTF*KCQKYDASYRLLKKKEKP AYFVQKISTSSLLFTRCFV*AYVSNTFFVWS*CL HDICAHLLSIEA**IIVSWCQRGPARTEVLASS CVNSHKCRGP*YLLS*VCLRVF*TLMDFHY*RK *NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS ISGSHWKTRTPRSTRN*RFPWISGSHWAGRQTG PPRTKGRHGSDGSPRTAGTPGTKRRKGSVWRVP TPGVPKQHASSSALQPNNCPEGRTEPGQHPRSTR APRPPWTKWTSGAPRTARAYGATWLTWASWTKG RPRDPGLPRPEGRTGHARDARQAWSQGGARNCR GWDEG*ARDPRNQG*EGG*RLPWASWPPGAEGRE RRCWQLHWRRQRGTWPSRAPWAPRAKGRSRCRW PGWPPRAARRQGGAWSSWRTGTRWPQGLQGRTR ERRDGGLQWKHQ*GSPGDPDAGLDGASWSSWANW PTWSSRDSRPEGGDWTARPSRTRWGKGTSR*TR RHGPSWSPRPPRKGWTSRSEGRKRAPREPRREG GKRGDRTSRLTGSWAARARGASRTSGAPRCSWTK GGSRTRWSKRRERLPGRKRRPWSPGTTRYSRTN WSSRPSGTKGREGQQRRPWDDRTNGSSWASWFT WTTRGQGKPGRRWLTSPRLLEQVMPLTLDWPVCV FVHRIFIFIQFSLFENARSMMHLTDY*KRKKNL HILYRKYQPLPFCLQDVLYKPMSLIHFLFGRNV CMIFVHIY*VSKLNKLLCPGAKGGQPELRCWLAH V*IHINVEVHDIC*ARCV*EYFKPLWIFIIKGN ETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP AFQGPTGRPGLPGQPGTRGFPGFPGPIGLDGKPG HPGPKGDMGLTGPPGQPGPQGQKGEKGQCGEYP HRECLSSMPAALRSSQIIALKGEQSQASIQGPP GPPGPPGPSGPLGHPGLPGPMGPPGLPGPPGPKG DPGIQGYHGRKGERGMPGMPGKHGAKGAPGIAV AGMKGEPGIPGTKGEKGAEGSPGLPGLLGQKGE KGDAGNSIGGGRGEPGPPGLPGPPGPKGEAGVDG QVGPPGQPGDKGERGAAGEQGPDGPKGSKGEPG KGEMVDYNGNINEALQEIRTLALMGPPGLPGQI GPPGAPGIPGQKGEIGLPGPPGHDGEKGPRGKPG DMGPPGPQGPPGKDGPPGVKGENGHPGSPGEKG EKGETGQAGSPVPGLPGPEGPPGPPGLQGVPGP KGEAGLDGAKGEKGFQGEKGDRGPLGLPGTPGPI GVPGPAGPKGERGSKGDPGMTGPTGAAGLPGLH GPPGDKGNRGEDGLPVQGCWNK*CL*PWIGLCV CLYIEYLFLYSFHFLKMPEV*CILQIIKKERKTC IFCTENINLFPFVYKMFCISLCL*YIFCLVVMS A*YLCTFIKYRSLINYCVLVPKGASQN*GAG*L MCEFT*M*RSMIFAKLGVSKSILNPYGFSLLKEM KHGN

Sequence NM_080801

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCC CCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATC CAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGG GTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGG CAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCC CCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGG TGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGC TCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCT CCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGA CAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGG ACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGT CCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCG AGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGA GAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCA GTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGA AAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAA GATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAAT TATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGC TAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA *AACQQLCAPAK*LP*RANRARPASKVHQGPQA PLDQVDLWGTQDCQGLWGHLAYLGLLDQRETQGS RATTAGRENGACQGCQASMEPRGRPELPWLG*R VSQGSQEPRVRRGLKAPLGFLASWGRRERKAML ATPLEEAEGNLALQGSLGPQGQREKQVSMARLAP QGSQETRGSVEQLENRDQMAPRAPRENQGKERW WITMETSMRLSRRSGRWP*WGLLVFLGKLAHLE LQGFQARRGRLDCQALQDTMGKRDLAVNQETWAL LVPKAPQERMDLQE*REKTGTQGAQERRGKKGR QDKQAHRERKEKPGRRAIQEQRFLGCQGQRGLP DLRGSKVFLDQRGKQD*MEQKERKASREKKETVV PWDYPELQVWTAGLGHRVLQDQLEFQAQRDQRA RGAAKETLG*QDQREQLGFLVYMDHPGTRETGG RGGRKALEGLKGIRETKERLD*MPPAHWAKMAYQ SKAAGTSDASNLGLACVCVCT*NIYFYTVFTF* KCQKYDASYRLLKKKEKPAYFVQKISTSSLLFT RCFV*AYVSNTFFVWS*CLHDICAHLLSIEA**I IVSWCQRGPARTEVLASSCVNSHKCRGP*YLLS *VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP KQHASSSALQPNNCPEGRTEPGQHPRSTRAPRP PWTKWTSGAPRTARAYGATWLTWASWTKGRPRD PGLPRPEGRTGHARDARQAWSQGGARNCRGWDEG *ARDPRNQG*EGG*RLPWASWPPGAEGRERRCW QLHWRRQRGTWPSRAPWAPRAKGRSRCRWPGWP PRAARRQGGAWSSWRTGTRWPQGLQGRTRERRDG GLQWKHQ*GSPGDPDAGLDGASWSSWANWPTWS SRDSRPEGGDWTARPSRTRWGKGTSR*TRRHGP SWSPRPPRKGWTSRSEGRKRAPREPRREGGKRGD RTSRLTGRERRSRGEGQSRSRGSWAARARGASR TSGAPRCSWTKGGSRTRWSKRRERLPGRKRRPW SPGTTRSFRFGRQAWATGYSRTNWSSRPSGTKGR EGQQRRPWDDRTNGSSWASWFTWTTRGQGKPGG EGEERL*RA*RG*GRPRSAWIRCPLPIGRRWLT SPRLLEQVMPLTLDWPVCVFVHRIFIFIQFSLFE NARSMMHLTDY*KRKKNLHILYRKYQPLPFCLQ DVLYKPMSLIHFLFGRNVCMIFVHIY*VSKLNK LLCPGAKGGQPELRCWLAHV*IHINVEVHDIC*A RCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC LSSMPAALRSSQIIALKGEQSQASIQGPPGPPGP PGPSGPLGHPGLPGPMGPPGLPGPPGPKGDPGI QGYHGRKGERGMPGMPGKHGAKGAPGIAVAGMK GEPGIPGTKGEKGAEGSPGLPGLLGQKGEKGDAG NSIGGGRGEPGPPGLPGPPGPKGEAGVDGQVGP PGQPGDKGERGAAGEQGPDGPKGSKGEPGKGEM VDYNGNINEALQEIRTLALMGPPGLPGQIGPPGA PGIPGQKGEIGLPGPPGHDGEKGPRGKPGDMGP PGPQGPPGKDGPPGVKGENGHPGSPGEKGEKGE TGQAGSPGEKGEAGEKGNPGAEVPGLPGPEGPPG PPGLQGVPGPKGEAGLDGAKGEKGFQGEKGDRG PLGLPGASGLDGRPGPPGTPGPIGVPGPAGPKG ERGSKGDPGMTGPTGAAGLPGLHGPPGDKGNRGE RGKKGSRGPKGDKGDQGAPGLDAPCPLGEDGLP VQGCWNK*CL*PWIGLCVCLYIEYLFLYSFHFL KMPEV*CILQIIKKERKTCIFCTENINLFPFVYK MFCISLCL*YIFCLVVMSA*YLCTFIKYRSLIN YCVLVPKGASQN*GAG*LMCEFT*M*RSMIFAK LGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080812

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG TTTTTCAGGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCAAAGGGCGACATGGG TCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGCTGCTGCCTCTCCTCAATTCA GTGCGACTGGCTCCACCCCCGGTCATAAAAAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTG GACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGG CTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAG CCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACT CCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGG GCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGAT TACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAG GGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGG TCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGA CAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTC CGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCT GGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGG GGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGG GGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCA AGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATG CCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGT TTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTG TGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGT GTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ FFRVNQELEVSLDFRVPLGWTANRATQDQRATW V*RVPQDSRDPRDKKEKRVSVESTHTGCCLSSIQ CDWLHPRS*KGGRSRANRARPASKVHQGPQAPL DQVDLWGTQDCQGLWGHLAYLGLLDQRETQGSR ATTAGRENGACQGCQASMEPRGRPELPWLG*RVS QGSQEPRVRRGLKAPLGFLASWGRRERKAMLAT PLEEAEGNLALQGSLGPQGQREKQVSMARLAPQ GSQETRGSVEQLENRDQMAPRAPRENQGKERWWI TMETSMRLSRRSGRWP*WGLLVFLGKLAHLELQ GFQARRGRLDCQALQDTMGKRDLAVNQETWALL VPKAPQERMDLQE*REKTGTQGAQERRGKKGRQD KQAHRERKEKPGRRAIQEQRFLGCQGQRGLPDL RGSKVFLDQRGKQD*MEQKERKASREKKETVVP WDYPELQVWTAGLGHRVLQDQLEFQAQRDQRARG AAKETLG*QDQREQLGFLVYMDHPGTRETGGRG GRKALEGLKGIRETKERLD*MPPAHWAKMAYQS KAAGTSDASNLGLACVCVCT*NIYFYTVFTF*KC QKYDASYRLLKKKEKPAYFVQKISTSSLLFTRC FV*AYVSNTFFVWS*CLHDICAHLLSIEA**II VSWCQRGPARTEVLASSCVNSHKCRGP*YLLS*V CLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS FSGSTRN*RFPWISGSHWAGRQTGPPRTKGRHG SDGSPRTAGTPGTKRRKGSVWRVPTPAAASPQF SATGSTPGHKKADVPGRTEPGQHPRSTRAPRPPW TKWTSGAPRTARAYGATWLTWASWTKGRPRDPG LPRPEGRTGHARDARQAWSQGGARNCRGWDEG* ARDPRNQG*EGG*RLPWASWPPGAEGRERRCWQL HWRRQRGTWPSRAPWAPRAKGRSRCRWPGWPPR AARRQGGAWSSWRTGTRWPQGLQGRTRERRDGG LQWKHQ*GSPGDPDAGLDGASWSSWANWPTWSSR DSRPEGGDWTARPSRTRWGKGTSR*TRRHGPSW SPRPPRKGWTSRSEGRKRAPREPRREGGKRGDR TSRLTGRERRSRGEGQSRSRGSWAARARGASRTS GAPRCSWTKGGSRTRWSKRRERLPGRKRRPWSP GTTRSFRFGRQAWATGYSRTNWSSRPSGTKGRE GQQRRPWDDRTNGSSWASWFTWTTRGQGKPGGEG EERL*RA*RG*GRPRSAWIRCPLPIGRRWLTSP RLLEQVMPLTLDWPVCVFVHRIFIFIQFSLFEN ARSMMHLTDY*KRKKNLHILYRKYQPLPFCLQDV LYKPMSLIHFLFGRNVCMIFVHIY*VSKLNKLL CPGAKGGQPELRCWLAHV*IHINVEVHDIC*AR CV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP VFQGQPGTRGFPGFPGPIGLDGKPGHPGPKGDMG LTGPPGQPGPQGQKGEKGQCGEYPHRLLPLLNS VRLAPPPVIKRRTFQGEQSQASIQGPPGPPGPP GPSGPLGHPGLPGPMGPPGLPGPPGPKGDPGIQG YHGRKGERGMPGMPGKHGAKGAPGIAVAGMKGE PGIPGTKGEKGAEGSPGLPGLLGQKGEKGDAGN SIGGGRGEPGPPGLPGPPGPKGEAGVDGQVGPPG QPGDKGERGAAGEQGPDGPKGSKGEPGKGEMVD YNGNINEALQEIRTLALMGPPGLPGQIGPPGAP GIPGQKGEIGLPGPPGHDGEKGPRGKPGDMGPPG PQGPPGKDGPPGVKGENGHPGSPGEKGEKGETG QAGSPGEKGEAGEKGNPGAEVPGLPGPEGPPGP PGLQGVPGPKGEAGLDGAKGEKGFQGEKGDRGPL GLPGASGLDGRPGPPGTPGPIGVPGPAGPKGER GSKGDPGMTGPTGAAGLPGLHGPPGDKGNRGER GKKGSRGPKGDKGDQGAPGLDAPCPLGEDGLPVQ GCWNK*CL*PWIGLCVCLYIEYLFLYSFHFLKM PEV*CILQIIKKERKTCIFCTENINLFPFVYKM FCISLCL*YIFCLVVMSA*YLCTFIKYRSLINYC VLVPKGASQN*GAG*LMCEFT*M*RSMIFAKLG VSKSILNPYGFSLLKEMKHGN

Sequence NM_080806

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGGCGAAC AGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACC TGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCAT GGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGC TTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCC AGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGAT GGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGA TGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGA TGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAAC GGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAG GAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGC AAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACT CCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTC CTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCC TGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGT TTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCA TATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGC ATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATG TGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGA AACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ RATWV*RVPQDSRDPRDKKEKRVSVESTHTGAN RARPASKVHQGPQAPLDQVDLWGTQDCQGLWGH LAYLGLLDQRETQGSRATTAGRENGACQGCQASM EPRGRPELPWLG*RVSQGSQEPRVRRGLKAPLG FLASWGRRERKAMLATPLEEAEGNLALQGSLGP QGQREKQVSMARLAPQGSQETRGSVEQLENRDQM APRAPRENQGKERWWITMETSMRLSRRSGRWP* WGLLVFLGKLAHLELQGFQARRGRLDCQALQDT MGKRDLAVNQETWALLVPKAPQERMDLQE*REKT GTQGAQERRGKKGRQDKQAHRERKEKPGRRAIQ EQRFLGCQGQRGLPDLRGSKVFLDQRGKQD*ME QKERKASREKKETVVPWDYPELQVWTAGLGHRVL QDQLEFQAQRDQRARGAAKETLG*QDQREQLGF LVYMDHPGTRETGGRGGRKALEGLKGIRETKER LD*MPPAHWAKMAYQSKAAGTSDASNLGLACVCV CT*NIYFYTVFTF*KCQKYDASYRLLKKKEKPA YFVQKISTSSLLFTRCFV*AYVSNTFFVWS*CL HDICAHLLSIEA**IIVSWCQRGPARTEVLASSC VNSHKCRGP*YLLS*VCLRVF*TLMDFHY*RK* NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGRT EPGQHPRSTRAPRPPWTKWTSGAPRTARAYGAT WLTWASWTKGRPRDPGLPRPEGRTGHARDARQA WSQGGARNCRGWDEG*ARDPRNQG*EGG*RLPWA SWPPGAEGRERRCWQLHWRRQRGTWPSRAPWAP RAKGRSRCRWPGWPPRAARRQGGAWSSWRTGTR WPQGLQGRTRERRDGGLQWKHQ*GSPGDPDAGLD GASWSSWANWPTWSSRDSRPEGGDWTARPSRTR WGKGTSR*TRRHGPSWSPRPPRKGWTSRSEGRK RAPREPRREGGKRGDRTSRLTGRERRSRGEGQSR SRGSWAARARGASRTSGAPRCSWTKGGSRTRWS KRRERLPGRKRRPWSPGTTRSFRFGRQAWATGY SRTNWSSRPSGTKGREGQQRRPWDDRTNGSSWAS WFTWTTRGQGKPGGEGEERL*RA*RG*GRPRSA WIRCPLPIGRRWLTSPRLLEQVMPLTLDWPVCV FVHRIFIFIQFSLFENARSMMHLTDY*KRKKNLH ILYRKYQPLPFCLQDVLYKPMSLIHFLFGRNVC MIFVHIY*VSKLNKLLCPGAKGGQPELRCWLAH V*IHINVEVHDIC*ARCV*EYFKPLWIFIIKGNE TWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHRGE QSQASIQGPPGPPGPPGPSGPLGHPGLPGPMGPP GLPGPPGPKGDPGIQGYHGRKGERGMPGMPGKH GAKGAPGIAVAGMKGEPGIPGTKGEKGAEGSPG LPGLLGQKGEKGDAGNSIGGGRGEPGPPGLPGPP GPKGEAGVDGQVGPPGQPGDKGERGAAGEQGPD GPKGSKGEPGKGEMVDYNGNINEALQEIRTLAL MGPPGLPGQIGPPGAPGIPGQKGEIGLPGPPGHD GEKGPRGKPGDMGPPGPQGPPGKDGPPGVKGEN GHPGSPGEKGEKGETGQAGSPGEKGEAGEKGNP GAEVPGLPGPEGPPGPPGLQGVPGPKGEAGLDGA KGEKGFQGEKGDRGPLGLPGASGLDGRPGPPGT PGPIGVPGPAGPKGERGSKGDPGMTGPTGAAGL PGLHGPPGDKGNRGERGKKGSRGPKGDKGDQGAP GLDAPCPLGEDGLPVQGCWNK*CL*PWIGLCVC LYIEYLFLYSFHFLKMPEV*CILQIIKKERKTC IFCTENINLFPFVYKMFCISLCL*YIFCLVVMSA *YLCTFIKYRSLINYCVLVPKGASQN*GAG*LM CEFT*M*RSMIFAKLGVSKSILNPYGFSLLKEM KHGN

Sequence NM_080809

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGTTCCTGGGCTGCC AGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAG GGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAG GAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGG CTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGG AACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTAT GATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAG CCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTG CCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGA GTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA *AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS* KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE QLENRDQMAPRAPRENQGKERWWITMETSMRLS RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD CQALQDTMGKRDLAVNQETWALLVPKAPQERMD LQE*REKTGTQGAQERRGKKGRQDKQAHRFLGC QGQRGLPDLRGSKVFLDQRGKQD*MEQKERKASR EKKETVVPWDYPVLQDQLEFQAQRDQRARGAAK ETLG*QDQREQLGFLVYMDHPGTRETGGRGGRK ALEGLKGIRETKERLD*MPPAHWAKMAYQSKAAG TSDASNLGLACVCVCT*NIYFYTVFTF*KCQKY DASYRLLKKKEKPAYFVQKISTSSLLFTRCFV* AYVSNTFFVWS*CLHDICAHLLSIEA**IIVSWC QRGPARTEVLASSCVNSHKCRGP*YLLS*VCLR VF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP KQHASSSALQPNNCPEAAASPQFSATGSTPGHK KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT SRSEGRKRAPREPRREGGKRGDRTSRLTGSWAA RARGASRTSGAPRCSWTKGGSRTRWSKRRERLP GRKRRPWSPGTTRYSRTNWSSRPSGTKGREGQQR RPWDDRTNGSSWASWFTWTTRGQGKPGGEGEER L*RA*RG*GRPRSAWIRCPLPIGRRWLTSPRLL EQVMPLTLDWPVCVFVHRIFIFIQFSLFENARSM MHLTDY*KRKKNLHILYRKYQPLPFCLQDVLYK PMSLIHFLFGRNVCMIFVHIY*VSKLNKLLCPG AKGGQPELRCWLAHV*IHINVEVHDIC*ARCV*E YFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG PPGVKGENGHPGSPGEKGEKGETGQAGSPVPGLP GPEGPPGPPGLQGVPGPKGEAGLDGAKGEKGFQ GEKGDRGPLGLPGTPGPIGVPGPAGPKGERGSK GDPGMTGPTGAAGLPGLHGPPGDKGNRGERGKKG SRGPKGDKGDQGAPGLDAPCPLGEDGLPVQGCW NK*CL*PWIGLCVCLYIEYLFLYSFHFLKMPEV *CILQIIKKERKTCIFCTENINLFPFVYKMFCIS LCL*YIFCLVVMSA*YLCTFIKYRSLINYCVLV PKGASQN*GAG*LMCEFT*M*RSMIFAKLGVSK SILNPYGFSLLKEMKHGN

Sequence NM_080810

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGTCCCCCGGAGACGCTGGGCTGTCCATCATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAAC TAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCAAAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAG CCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCC CAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCC AGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGG ATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCG ATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGT TGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGA GAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCAC CTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACAT GGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAA GGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGC CTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGA CCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCA AAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACC GGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGG CTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCAC TTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTG TTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTT AATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTT GCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ HFRVPLEDPDSQGPPETLGCPSLVPAAPLVNQE LEVSLDFRVPLGWTANRATQDQRATWV*RVPQDS RDPRDKKEKRVSVESTHTGANRARPASKVHQGP QAPLDQVDLWGTQDCQGLWGHLAYLGLLDQRET QGSRATTAGRENGACQGCQASMEPRGRPELPWLG *RVSQGSQEPRVRRGLKAPLGFLASWGRRERKA MLATPLEEAEGNLALQGSLGPQGQREKQVSMAR LAPQGSQETRGSVEQLENRDQMAPRAPRENQGKE RWWITMETSMRLSRRSGRWP*WGLLVFLGKLAH LELQGFQARRGRLDCQALQDTMGKRDLAVNQET WALLVPKAPQERMDLQE*REKTGTQGAQERRGKK GRQDKQAHRERKEKPGRRAIQEQRFLGCQGQRG LPDLRGSKVFLDQRGKQD*MEQKERKASREKKE TVVPWDYPELQVWTAGLGHRVLQDQLEFQAQRDQ RARGAAKETLG*QDQREQLGFLVYMDHPGTRET GGRGGRKALEGLKGIRETKERLD*MPPAHWAKM AYQSKAAGTSDASNLGLACVCVCT*NIYFYTVFT F*KCQKYDASYRLLKKKEKPAYFVQKISTSSLL FTRCFV*AYVSNTFFVWS*CLHDICAHLLSIEA **IIVSWCQRGPARTEVLASSCVNSHKCRGP*YL LS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS ISGSHWKTRTPRVPRRRWAVHHWSPRPPWSTRN *RFPWISGSHWAGRQTGPPRTKGRHGSDGSPRT AGTPGTKRRKGSVWRVPTPGRTEPGQHPRSTRAP RPPWTKWTSGAPRTARAYGATWLTWASWTKGRP RDPGLPRPEGRTGHARDARQAWSQGGARNCRGW DEG*ARDPRNQG*EGG*RLPWASWPPGAEGRERR CWQLHWRRQRGTWPSRAPWAPRAKGRSRCRWPG WPPRAARRQGGAWSSWRTGTRWPQGLQGRTRER RDGGLQWKHQ*GSPGDPDAGLDGASWSSWANWPT WSSRDSRPEGGDWTARPSRTRWGKGTSR*TRRH GPSWSPRPPRKGWTSRSEGRKRAPREPRREGGK RGDRTSRLTGRERRSRGEGQSRSRGSWAARARGA SRTSGAPRCSWTKGGSRTRWSKRRERLPGRKRR PWSPGTTRSFRFGRQAWATGYSRTNWSSRPSGT KGREGQQRRPWDDRTNGSSWASWFTWTTRGQGKP GGEGEERL*RA*RG*GRPRSAWIRCPLPIGRRW LTSPRLLEQVMPLTLDWPVCVFVHRIFIFIQFS LFENARSMMHLTDY*KRKKNLHILYRKYQPLPFC LQDVLYKPMSLIHFLFGRNVCMIFVHIY*VSKL NKLLCPGAKGGQPELRCWLAHV*IHINVEVHDI C*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP AFQGPTGRPGLPGSPGDAGLSIIGPRGPPGQPGT RGFPGFPGPIGLDGKPGHPGPKGDMGLTGPPGQ PGPQGQKGEKGQCGEYPHRGEQSQASIQGPPGP PGPPGPSGPLGHPGLPGPMGPPGLPGPPGPKGDP GIQGYHGRKGERGMPGMPGKHGAKGAPGIAVAG MKGEPGIPGTKGEKGAEGSPGLPGLLGQKGEKG DAGNSIGGGRGEPGPPGLPGPPGPKGEAGVDGQV GPPGQPGDKGERGAAGEQGPDGPKGSKGEPGKG EMVDYNGNINEALQEIRTLALMGPPGLPGQIGP PGAPGIPGQKGEIGLPGPPGHDGEKGPRGKPGDM GPPGPQGPPGKDGPPGVKGENGHPGSPGEKGEK GETGQAGSPGEKGEAGEKGNPGAEVPGLPGPEG PPGPPGLQGVPGPKGEAGLDGAKGEKGFQGEKGD RGPLGLPGASGLDGRPGPPGTPGPIGVPGPAGP KGERGSKGDPGMTGPTGAAGLPGLHGPPGDKGN RGERGKKGSRGPKGDKGDQGAPGLDAPCPLGEDG LPVQGCWNK*CL*PWIGLCVCLYIEYLFLYSFH FLKMPEV*CILQIIKKERKTCIFCTENINLFPF VYKMFCISLCL*YIFCLVVMSA*YLCTFIKYRSL INYCVLVPKGASQN*GAG*LMCEFT*M*RSMIF AKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080811

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCC CCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATC CAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGG GTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGG CAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCC CCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGG TGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGC TCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCT CCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGA CAGGACAAGCAGGCTCACCGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGG ACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGTACTCCAGGACCAATTGGAGTTCCAGGC CCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGG ACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATT GGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTA TACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACC TCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAA GTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGG TCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA *AACQQLCAPAK*LP*RANRARPASKVHQGPQA PLDQVDLWGTQDCQGLWGHLAYLGLLDQRETQGS RATTAGRENGACQGCQASMEPRGRPELPWLG*R VSQGSQEPRVRRGLKAPLGFLASWGRRERKAML ATPLEEAEGNLALQGSLGPQGQREKQVSMARLAP QGSQETRGSVEQLENRDQMAPRAPRENQGKERW WITMETSMRLSRRSGRWP*WGLLVFLGKLAHLE LQGFQARRGRLDCQALQDTMGKRDLAVNQETWAL LVPKAPQERMDLQE*REKTGTQGAQERRGKKGR QDKQAHRFLGCQGQRGLPDLRGSKVFLDQRGKQ D*MEQKERKASREKKETVVPWDYPVLQDQLEFQA QRDQRARGAAKETLG*QDQREQLGFLVYMDHPG TRETGGRGGRKALEGLKGIRETKERLD*MPPAH WAKMAYQSKAAGTSDASNLGLACVCVCT*NIYFY TVFTF*KCQKYDASYRLLKKKEKPAYFVQKIST SSLLFTRCFV*AYVSNTFFVWS*CLHDICAHLL SIEA**IIVSWCQRGPARTEVLASSCVNSHKCRG P*YLLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP KQHASSSALQPNNCPEGRTEPGQHPRSTRAPRP PWTKWTSGAPRTARAYGATWLTWASWTKGRPRD PGLPRPEGRTGHARDARQAWSQGGARNCRGWDEG *ARDPRNQG*EGG*RLPWASWPPGAEGRERRCW QLHWRRQRGTWPSRAPWAPRAKGRSRCRWPGWP PRAARRQGGAWSSWRTGTRWPQGLQGRTRERRDG GLQWKHQ*GSPGDPDAGLDGASWSSWANWPTWS SRDSRPEGGDWTARPSRTRWGKGTSR*TRRHGP SWSPRPPRKGWTSRSEGRKRAPREPRREGGKRGD RTSRLTGSWAARARGASRTSGAPRCSWTKGGSR TRWSKRRERLPGRKRRPWSPGTTRYSRTNWSSR PSGTKGREGQQRRPWDDRTNGSSWASWFTWTTRG QGKPGGEGEERL*RA*RG*GRPRSAWIRCPLPI GRRWLTSPRLLEQVMPLTLDWPVCVFVHRIFIF IQFSLFENARSMMHLTDY*KRKKNLHILYRKYQP LPFCLQDVLYKPMSLIHFLFGRNVCMIFVHIY* VSKLNKLLCPGAKGGQPELRCWLAHV*IHINVE VHDIC*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC LSSMPAALRSSQIIALKGEQSQASIQGPPGPPGP PGPSGPLGHPGLPGPMGPPGLPGPPGPKGDPGI QGYHGRKGERGMPGMPGKHGAKGAPGIAVAGMK GEPGIPGTKGEKGAEGSPGLPGLLGQKGEKGDAG NSIGGGRGEPGPPGLPGPPGPKGEAGVDGQVGP PGQPGDKGERGAAGEQGPDGPKGSKGEPGKGEM VDYNGNINEALQEIRTLALMGPPGLPGQIGPPGA PGIPGQKGEIGLPGPPGHDGEKGPRGKPGDMGP PGPQGPPGKDGPPGVKGENGHPGSPGEKGEKGE TGQAGSPVPGLPGPEGPPGPPGLQGVPGPKGEAG LDGAKGEKGFQGEKGDRGPLGLPGTPGPIGVPG PAGPKGERGSKGDPGMTGPTGAAGLPGLHGPPG DKGNRGERGKKGSRGPKGDKGDQGAPGLDAPCPL GEDGLPVQGCWNK*CL*PWIGLCVCLYIEYLFL YSFHFLKMPEV*CILQIIKKERKTCIFCTENIN LFPFVYKMFCISLCL*YIFCLVVMSA*YLCTFIK YRSLINYCVLVPKGASQN*GAG*LMCEFT*M*R SMIFAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080798

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGTCCCCCGGAGACGCTGGGCTGTCCATCATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAAC TAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCAAAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAG CCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCCTAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAA TAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAAAAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAG CATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGG CCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGG CGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCT GGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGA GAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCT CCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGG TCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGA CCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGA GCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCC TGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAA GGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTG GAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGG ACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCC CCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAAT ATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGA AAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGC ACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACAT AAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ HFRVPLEDPDSQGPPETLGCPSLVPAAPLVNQE LEVSLDFRVPLGWTANRATQDQRATWV*RVPQDS RDPRDKKEKRVSVESTHTGSA*AACQQLCAPAK *LP*SCCLSSIQCDWLHPRS*KGGRSRANRARP ASKVHQGPQAPLDQVDLWGTQDCQGLWGHLAYLG LLDQRETQGSRATTAGRENGACQGCQASMEPRG RPELPWLG*RVSQGSQEPRVRRGLKAPLGFLAS WGRRERKAMLATPLEEAEGNLALQGSLGPQGQRE KQVSMARLAPQGSQETRGSVEQLENRDQMAPRA PRENQGKERWWITMETSMRLSRRSGRWP*WGLL VFLGKLAHLELQGFQARRGRLDCQALQDTMGKRD LAVNQETWALLVPKAPQERMDLQE*REKTGTQG AQERRGKKGRQDKQAHRERKEKPGRRAIQEQRF LGCQGQRGLPDLRGSKVFLDQRGKQD*MEQKERK ASREKKETVVPWDYPELQVWTAGLGHRVLQDQL EFQAQRDQRARGAAKETLG*QDQREQLGFLVYM DHPGTRETGGRGGRKALEGLKGIRETKERLD*MP PAHWAKMAYQSKAAGTSDASNLGLACVCVCT*N IYFYTVFTF*KCQKYDASYRLLKKKEKPAYFVQ KISTSSLLFTRCFV*AYVSNTFFVWS*CLHDICA HLLSIEA**IIVSWCQRGPARTEVLASSCVNSH KCRGP*YLLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS ISGSHWKTRTPRVPRRRWAVHHWSPRPPWSTRN *RFPWISGSHWAGRQTGPPRTKGRHGSDGSPRT AGTPGTKRRKGSVWRVPTPGVPKQHASSSALQPN NCPEAAASPQFSATGSTPGHKKADVPGRTEPGQ HPRSTRAPRPPWTKWTSGAPRTARAYGATWLTW ASWTKGRPRDPGLPRPEGRTGHARDARQAWSQGG ARNCRGWDEG*ARDPRNQG*EGG*RLPWASWPP GAEGRERRCWQLHWRRQRGTWPSRAPWAPRAKG RSRCRWPGWPPRAARRQGGAWSSWRTGTRWPQGL QGRTRERRDGGLQWKHQ*GSPGDPDAGLDGASW SSWANWPTWSSRDSRPEGGDWTARPSRTRWGKG TSR*TRRHGPSWSPRPPRKGWTSRSEGRKRAPRE PRREGGKRGDRTSRLTGRERRSRGEGQSRSRGS WAARARGASRTSGAPRCSWTKGGSRTRWSKRRE RLPGRKRRPWSPGTTRSFRFGRQAWATGYSRTNW SSRPSGTKGREGQQRRPWDDRTNGSSWASWFTW TTRGQGKPGGEGEERL*RA*RG*GRPRSAWIRC PLPIGRRWLTSPRLLEQVMPLTLDWPVCVFVHRI FIFIQFSLFENARSMMHLTDY*KRKKNLHILYR KYQPLPFCLQDVLYKPMSLIHFLFGRNVCMIFV HIY*VSKLNKLLCPGAKGGQPELRCWLAHV*IHI NVEVHDIC*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP AFQGPTGRPGLPGSPGDAGLSIIGPRGPPGQPGT RGFPGFPGPIGLDGKPGHPGPKGDMGLTGPPGQ PGPQGQKGEKGQCGEYPHRECLSSMPAALRSSQ IIALKLLPLLNSVRLAPPPVIKRRTFQGEQSQAS IQGPPGPPGPPGPSGPLGHPGLPGPMGPPGLPG PPGPKGDPGIQGYHGRKGERGMPGMPGKHGAKG APGIAVAGMKGEPGIPGTKGEKGAEGSPGLPGLL GQKGEKGDAGNSIGGGRGEPGPPGLPGPPGPKG EAGVDGQVGPPGQPGDKGERGAAGEQGPDGPKG SKGEPGKGEMVDYNGNINEALQEIRTLALMGPPG LPGQIGPPGAPGIPGQKGEIGLPGPPGHDGEKG PRGKPGDMGPPGPQGPPGKDGPPGVKGENGHPG SPGEKGEKGETGQAGSPGEKGEAGEKGNPGAEVP GLPGPEGPPGPPGLQGVPGPKGEAGLDGAKGEK GFQGEKGDRGPLGLPGASGLDGRPGPPGTPGPI GVPGPAGPKGERGSKGDPGMTGPTGAAGLPGLHG PPGDKGNRGERGKKGSRGPKGDKGDQGAPGLDA PCPLGEDGLPVQGCWNK*CL*PWIGLCVCLYIE YLFLYSFHFLKMPEV*CILQIIKKERKTCIFCTE NINLFPFVYKMFCISLCL*YIFCLVVMSA*YLC TFIKYRSLINYCVLVPKGASQN*GAG*LMCEFT *M*RSMIFAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080800

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGCTGCTGC CTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAAAAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCC CCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGAC CCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTG GGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGG CGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAG GTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAG GAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCC ACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGAC ATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAA AAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGG GCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGA GACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGAC CAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAA CCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGAT GGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTC ACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTT TGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGC TTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATAT TTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ RATWV*RVPQDSRDPRDKKEKRVSVESTHTGCC LSSIQCDWLHPRS*KGGRSRANRARPASKVHQG PQAPLDQVDLWGTQDCQGLWGHLAYLGLLDQRET QGSRATTAGRENGACQGCQASMEPRGRPELPWL G*RVSQGSQEPRVRRGLKAPLGFLASWGRRERK AMLATPLEEAEGNLALQGSLGPQGQREKQVSMAR LAPQGSQETRGSVEQLENRDQMAPRAPRENQGK ERWWITMETSMRLSRRSGRWP*WGLLVFLGKLA HLELQGFQARRGRLDCQALQDTMGKRDLAVNQET WALLVPKAPQERMDLQE*REKTGTQGAQERRGK KGRQDKQAHRERKEKPGRRAIQEQRFLGCQGQR GLPDLRGSKVFLDQRGKQD*MEQKERKASREKKE TVVPWDYPELQVWTAGLGHRVLQDQLEFQAQRD QRARGAAKETLG*QDQREQLGFLVYMDHPGTRE TGGRGGRKALEGLKGIRETKERLD*MPPAHWAKM AYQSKAAGTSDASNLGLACVCVCT*NIYFYTVF TF*KCQKYDASYRLLKKKEKPAYFVQKISTSSL LFTRCFV*AYVSNTFFVWS*CLHDICAHLLSIEA **IIVSWCQRGPARTEVLASSCVNSHKCRGP*Y LLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPAAA SPQFSATGSTPGHKKADVPGRTEPGQHPRSTRA PRPPWTKWTSGAPRTARAYGATWLTWASWTKGR PRDPGLPRPEGRTGHARDARQAWSQGGARNCRGW DEG*ARDPRNQG*EGG*RLPWASWPPGAEGRER RCWQLHWRRQRGTWPSRAPWAPRAKGRSRCRWP GWPPRAARRQGGAWSSWRTGTRWPQGLQGRTRER RDGGLQWKHQ*GSPGDPDAGLDGASWSSWANWP TWSSRDSRPEGGDWTARPSRTRWGKGTSR*TRR HGPSWSPRPPRKGWTSRSEGRKRAPREPRREGGK RGDRTSRLTGRERRSRGEGQSRSRGSWAARARG ASRTSGAPRCSWTKGGSRTRWSKRRERLPGRKR RPWSPGTTRSFRFGRQAWATGYSRTNWSSRPSGT KGREGQQRRPWDDRTNGSSWASWFTWTTRGQGK PGGEGEERL*RA*RG*GRPRSAWIRCPLPIGRR WLTSPRLLEQVMPLTLDWPVCVFVHRIFIFIQFS LFENARSMMHLTDY*KRKKNLHILYRKYQPLPF CLQDVLYKPMSLIHFLFGRNVCMIFVHIY*VSK LNKLLCPGAKGGQPELRCWLAHV*IHINVEVHDI C*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHRLL PLLNSVRLAPPPVIKRRTFQGEQSQASIQGPPGP PGPPGPSGPLGHPGLPGPMGPPGLPGPPGPKGD PGIQGYHGRKGERGMPGMPGKHGAKGAPGIAVA GMKGEPGIPGTKGEKGAEGSPGLPGLLGQKGEKG DAGNSIGGGRGEPGPPGLPGPPGPKGEAGVDGQ VGPPGQPGDKGERGAAGEQGPDGPKGSKGEPGK GEMVDYNGNINEALQEIRTLALMGPPGLPGQIGP PGAPGIPGQKGEIGLPGPPGHDGEKGPRGKPGD MGPPGPQGPPGKDGPPGVKGENGHPGSPGEKGE KGETGQAGSPGEKGEAGEKGNPGAEVPGLPGPEG PPGPPGLQGVPGPKGEAGLDGAKGEKGFQGEKG DRGPLGLPGASGLDGRPGPPGTPGPIGVPGPAG PKGERGSKGDPGMTGPTGAAGLPGLHGPPGDKGN RGERGKKGSRGPKGDKGDQGAPGLDAPCPLGED GLPVQGCWNK*CL*PWIGLCVCLYIEYLFLYSF HFLKMPEV*CILQIIKKERKTCIFCTENINLFPF VYKMFCISLCL*YIFCLVVMSA*YLCTFIKYRS LINYCVLVPKGASQN*GAG*LMCEFT*M*RSMI FAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080813

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG TTTTTCAGGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCAAAGGGCGACATGGG TCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCCTAAGCAGCATGCCA GCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTG GACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGG CCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATC CCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAG GAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGG AGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGA AACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAG GCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGG CCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGC TCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCC AAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACC CGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAA GGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAG GCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTG GAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTA TGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAA GCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGT GCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAG AGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ FFRVNQELEVSLDFRVPLGWTANRATQDQRATW V*RVPQDSRDPRDKKEKRVSVESTHTGSA*AACQ QLCAPAK*LP*RANRARPASKVHQGPQAPLDQV DLWGTQDCQGLWGHLAYLGLLDQRETQGSRATT AGRENGACQGCQASMEPRGRPELPWLG*RVSQGS QEPRVRRGLKAPLGFLASWGRRERKAMLATPLE EAEGNLALQGSLGPQGQREKQVSMARLAPQGSQ ETRGSVEQLENRDQMAPRAPRENQGKERWWITME TSMRLSRRSGRWP*WGLLVFLGKLAHLELQGFQ ARRGRLDCQALQDTMGKRDLAVNQETWALLVPK APQERMDLQE*REKTGTQGAQERRGKKGRQDKQA HRERKEKPGRRAIQEQRFLGCQGQRGLPDLRGS KVFLDQRGKQD*MEQKERKASREKKETVVPWDY PELQVWTAGLGHRVLQDQLEFQAQRDQRARGAAK ETLG*QDQREQLGFLVYMDHPGTRETGGRGGRK ALEGLKGIRETKERLD*MPPAHWAKMAYQSKAA GTSDASNLGLACVCVCT*NIYFYTVFTF*KCQKY DASYRLLKKKEKPAYFVQKISTSSLLFTRCFV* AYVSNTFFVWS*CLHDICAHLLSIEA**IIVSW CQRGPARTEVLASSCVNSHKCRGP*YLLS*VCLR VF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS FSGSTRN*RFPWISGSHWAGRQTGPPRTKGRHG SDGSPRTAGTPGTKRRKGSVWRVPTPGVPKQHA SSSALQPNNCPEGRTEPGQHPRSTRAPRPPWTKW TSGAPRTARAYGATWLTWASWTKGRPRDPGLPR PEGRTGHARDARQAWSQGGARNCRGWDEG*ARD PRNQG*EGG*RLPWASWPPGAEGRERRCWQLHWR RQRGTWPSRAPWAPRAKGRSRCRWPGWPPRAAR RQGGAWSSWRTGTRWPQGLQGRTRERRDGGLQW KHQ*GSPGDPDAGLDGASWSSWANWPTWSSRDSR PEGGDWTARPSRTRWGKGTSR*TRRHGPSWSPR PPRKGWTSRSEGRKRAPREPRREGGKRGDRTSR LTGRERRSRGEGQSRSRGSWAARARGASRTSGAP RCSWTKGGSRTRWSKRRERLPGRKRRPWSPGTT RSFRFGRQAWATGYSRTNWSSRPSGTKGREGQQ RRPWDDRTNGSSWASWFTWTTRGQGKPGGEGEER L*RA*RG*GRPRSAWIRCPLPIGRRWLTSPRLL EQVMPLTLDWPVCVFVHRIFIFIQFSLFENARS MMHLTDY*KRKKNLHILYRKYQPLPFCLQDVLYK PMSLIHFLFGRNVCMIFVHIY*VSKLNKLLCPG AKGGQPELRCWLAHV*IHINVEVHDIC*ARCV* EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP VFQGQPGTRGFPGFPGPIGLDGKPGHPGPKGDMG LTGPPGQPGPQGQKGEKGQCGEYPHRECLSSMP AALRSSQIIALKGEQSQASIQGPPGPPGPPGPS GPLGHPGLPGPMGPPGLPGPPGPKGDPGIQGYHG RKGERGMPGMPGKHGAKGAPGIAVAGMKGEPGI PGTKGEKGAEGSPGLPGLLGQKGEKGDAGNSIG GGRGEPGPPGLPGPPGPKGEAGVDGQVGPPGQPG DKGERGAAGEQGPDGPKGSKGEPGKGEMVDYNG NINEALQEIRTLALMGPPGLPGQIGPPGAPGIP GQKGEIGLPGPPGHDGEKGPRGKPGDMGPPGPQG PPGKDGPPGVKGENGHPGSPGEKGEKGETGQAG SPGEKGEAGEKGNPGAEVPGLPGPEGPPGPPGL QGVPGPKGEAGLDGAKGEKGFQGEKGDRGPLGLP GASGLDGRPGPPGTPGPIGVPGPAGPKGERGSK GDPGMTGPTGAAGLPGLHGPPGDKGNRGERGKK GSRGPKGDKGDQGAPGLDAPCPLGEDGLPVQGCW NK*CL*PWIGLCVCLYIEYLFLYSFHFLKMPEV *CILQIIKKERKTCIFCTENINLFPFVYKMFCI SLCL*YIFCLVVMSA*YLCTFIKYRSLINYCVLV PKGASQN*GAG*LMCEFT*M*RSMIFAKLGVSK SILNPYGFSLLKEMKHGN

Sequence NM_080808

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGA AGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTC CAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCA AAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAA AGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGC TGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAG TATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTAT AAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTG GTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTA AGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA *AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS* KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE QLENRDQMAPRAPRENQGKERWWITMETSMRLS RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD CQALQDTMGKRDLAVNQETWALLVPKAPQERMD LQE*REKTGTQGAQERRGKKGRQDKQAHRERKE KPGRRAIQEQRGSKVFLDQRGKQD*MEQKERKAS REKKETVVPWDYPVLQDQLEFQAQRDQRARGAA KETLG*QDQREQLGFLVYMDHPGTRETGGRGGR KALEGLKGIRETKERLD*MPPAHWAKMAYQSKAA GTSDASNLGLACVCVCT*NIYFYTVFTF*KCQK YDASYRLLKKKEKPAYFVQKISTSSLLFTRCFV *AYVSNTFFVWS*CLHDICAHLLSIEA**IIVSW CQRGPARTEVLASSCVNSHKCRGP*YLLS*VCL RVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP KQHASSSALQPNNCPEAAASPQFSATGSTPGHK KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT SRSEGRKRAPREPRREGGKRGDRTSRLTGRERR SRGEGQSRSRGAPRCSWTKGGSRTRWSKRRERL PGRKRRPWSPGTTRYSRTNWSSRPSGTKGREGQQ RRPWDDRTNGSSWASWFTWTTRGQGKPGGEGEE RL*RA*RG*GRPRSAWIRCPLPIGRRWLTSPRL LEQVMPLTLDWPVCVFVHRIFIFIQFSLFENARS MMHLTDY*KRKKNLHILYRKYQPLPFCLQDVLY KPMSLIHFLFGRNVCMIFVHIY*VSKLNKLLCP GAKGGQPELRCWLAHV*IHINVEVHDIC*ARCV* EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG PPGVKGENGHPGSPGEKGEKGETGQAGSPGEKGE AGEKGNPGAEGLQGVPGPKGEAGLDGAKGEKGF QGEKGDRGPLGLPGTPGPIGVPGPAGPKGERGS KGDPGMTGPTGAAGLPGLHGPPGDKGNRGERGKK GSRGPKGDKGDQGAPGLDAPCPLGEDGLPVQGC WNK*CL*PWIGLCVCLYIEYLFLYSFHFLKMPE V*CILQIIKKERKTCIFCTENINLFPFVYKMFCI SLCL*YIFCLVVMSA*YLCTFIKYRSLINYCVL VPKGASQN*GAG*LMCEFT*M*RSMIFAKLGVS KSILNPYGFSLLKEMKHGN

Sequence NM_080799

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGTCAACCAGGAACTAGAGGTTTCCCTGG ATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCAAAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGA CAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCCTAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGC TGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAAAAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACC AGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAG GGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCG TGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGA GAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGAT GGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAG GGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAAT TGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCA GGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGG GGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCC AGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAA AAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAG CGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAA GGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGC GAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACA GTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTT CCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTAT CGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCA TGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ HFRVPLEDPDSQGTKVPLGCLDVWVNQELEVSL DFRVPLGWTANRATQDQRATWV*RVPQDSRDPRD KKEKRVSVESTHTGSA*AACQQLCAPAK*LP*S CCLSSIQCDWLHPRS*KGGRSRANRARPASKVH QGPQAPLDQVDLWGTQDCQGLWGHLAYLGLLDQR ETQGSRATTAGRENGACQGCQASMEPRGRPELP WLG*RVSQGSQEPRVRRGLKAPLGFLASWGRRE RKAMLATPLEEAEGNLALQGSLGPQGQREKQVSM ARLAPQGSQETRGSVEQLENRDQMAPRAPRENQ GKERWWITMETSMRLSRRSGRWP*WGLLVFLGK LAHLELQGFQARRGRLDCQALQDTMGKRDLAVNQ ETWALLVPKAPQERMDLQE*REKTGTQGAQERR GKKGRQDKQAHRERKEKPGRRAIQEQRFLGCQG QRGLPDLRGSKVFLDQRGKQD*MEQKERKASREK KETVVPWDYPELQVWTAGLGHRVLQDQLEFQAQ RDQRARGAAKETLG*QDQREQLGFLVYMDHPGT RETGGRGGRKALEGLKGIRETKERLD*MPPAHWA KMAYQSKAAGTSDASNLGLACVCVCT*NIYFYT VFTF*KCQKYDASYRLLKKKEKPAYFVQKISTS SLLFTRCFV*AYVSNTFFVWS*CLHDICAHLLSI EA**IIVSWCQRGPARTEVLASSCVNSHKCRGP *YLLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS ISGSHWKTRTPRGQRCHWDAWTCGSTRN*RFPW ISGSHWAGRQTGPPRTKGRHGSDGSPRTAGTPG TKRRKGSVWRVPTPGVPKQHASSSALQPNNCPEA AASPQFSATGSTPGHKKADVPGRTEPGQHPRST RAPRPPWTKWTSGAPRTARAYGATWLTWASWTK GRPRDPGLPRPEGRTGHARDARQAWSQGGARNCR GWDEG*ARDPRNQG*EGG*RLPWASWPPGAEGR ERRCWQLHWRRQRGTWPSRAPWAPRAKGRSRCR WPGWPPRAARRQGGAWSSWRTGTRWPQGLQGRTR ERRDGGLQWKHQ*GSPGDPDAGLDGASWSSWAN WPTWSSRDSRPEGGDWTARPSRTRWGKGTSR*T RRHGPSWSPRPPRKGWTSRSEGRKRAPREPRREG GKRGDRTSRLTGRERRSRGEGQSRSRGSWAARA RGASRTSGAPRCSWTKGGSRTRWSKRRERLPGR KRRPWSPGTTRSFRFGRQAWATGYSRTNWSSRPS GTKGREGQQRRPWDDRTNGSSWASWFTWTTRGQ GKPGGEGEERL*RA*RG*GRPRSAWIRCPLPIG RRWLTSPRLLEQVMPLTLDWPVCVFVHRIFIFIQ FSLFENARSMMHLTDY*KRKKNLHILYRKYQPL PFCLQDVLYKPMSLIHFLFGRNVCMIFVHIY*V SKLNKLLCPGAKGGQPELRCWLAHV*IHINVEVH DIC*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP AFQGPTGRPGLPGDKGAIGMPGRVGQPGTRGFPG FPGPIGLDGKPGHPGPKGDMGLTGPPGQPGPQG QKGEKGQCGEYPHRECLSSMPAALRSSQIIALK LLPLLNSVRLAPPPVIKRRTFQGEQSQASIQGPP GPPGPPGPSGPLGHPGLPGPMGPPGLPGPPGPK GDPGIQGYHGRKGERGMPGMPGKHGAKGAPGIA VAGMKGEPGIPGTKGEKGAEGSPGLPGLLGQKGE KGDAGNSIGGGRGEPGPPGLPGPPGPKGEAGVD GQVGPPGQPGDKGERGAAGEQGPDGPKGSKGEP GKGEMVDYNGNINEALQEIRTLALMGPPGLPGQI GPPGAPGIPGQKGEIGLPGPPGHDGEKGPRGKP GDMGPPGPQGPPGKDGPPGVKGENGHPGSPGEK GEKGETGQAGSPGEKGEAGEKGNPGAEVPGLPGP EGPPGPPGLQGVPGPKGEAGLDGAKGEKGFQGE KGDRGPLGLPGASGLDGRPGPPGTPGPIGVPGP AGPKGERGSKGDPGMTGPTGAAGLPGLHGPPGDK GNRGERGKKGSRGPKGDKGDQGAPGLDAPCPLG EDGLPVQGCWNK*CL*PWIGLCVCLYIEYLFLY SFHFLKMPEV*CILQIIKKERKTCIFCTENINLF PFVYKMFCISLCL*YIFCLVVMSA*YLCTFIKY RSLINYCVLVPKGASQN*GAG*LMCEFT*M*RS MIFAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_005203

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGA AGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAG GGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACG GCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGG ACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGG GATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAAC CTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTAT TAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATT TTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGA ACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGG ATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA *AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS* KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE QLENRDQMAPRAPRENQGKERWWITMETSMRLS RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD CQALQDTMGKRDLAVNQETWALLVPKAPQERMD LQE*REKTGTQGAQERRGKKGRQDKQAHRERKE KPGRRAIQEQRFLGCQGQRGLPDLRGSKVFLDQR GKQD*MEQKERKASREKKETVVPWDYPELQVWT AGLGHRVLQDQLEFQAQRDQRARGAAKETLG*Q DQREQLGFLVYMDHPGTRETGGRGGRKALEGLKG IRETKERLD*MPPAHWAKMAYQSKAAGTSDASN LGLACVCVCT*NIYFYTVFTF*KCQKYDASYRL LKKKEKPAYFVQKISTSSLLFTRCFV*AYVSNTF FVWS*CLHDICAHLLSIEA**IIVSWCQRGPAR TEVLASSCVNSHKCRGP*YLLS*VCLRVF*TLM DFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP KQHASSSALQPNNCPEAAASPQFSATGSTPGHK KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT SRSEGRKRAPREPRREGGKRGDRTSRLTGRERR SRGEGQSRSRGSWAARARGASRTSGAPRCSWTK GGSRTRWSKRRERLPGRKRRPWSPGTTRSFRFGR QAWATGYSRTNWSSRPSGTKGREGQQRRPWDDR TNGSSWASWFTWTTRGQGKPGGEGEERL*RA*R G*GRPRSAWIRCPLPIGRRWLTSPRLLEQVMPLT LDWPVCVFVHRIFIFIQFSLFENARSMMHLTDY *KRKKNLHILYRKYQPLPFCLQDVLYKPMSLIH FLFGRNVCMIFVHIY*VSKLNKLLCPGAKGGQPE LRCWLAHV*IHINVEVHDIC*ARCV*EYFKPLW IFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG PPGVKGENGHPGSPGEKGEKGETGQAGSPGEKGE AGEKGNPGAEVPGLPGPEGPPGPPGLQGVPGPK GEAGLDGAKGEKGFQGEKGDRGPLGLPGASGLD GRPGPPGTPGPIGVPGPAGPKGERGSKGDPGMTG PTGAAGLPGLHGPPGDKGNRGERGKKGSRGPKG DKGDQGAPGLDAPCPLGEDGLPVQGCWNK*CL* PWIGLCVCLYIEYLFLYSFHFLKMPEV*CILQII KKERKTCIFCTENINLFPFVYKMFCISLCL*YI FCLVVMSA*YLCTFIKYRSLINYCVLVPKGASQ N*GAG*LMCEFT*M*RSMIFAKLGVSKSILNPYG FSLLKEMKHGN

Sequence NM_080802

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGTTCCTGGGCTGCC AGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAG GGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAG GCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGG GGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCA TTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTT TATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAA CCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATT AAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGA GGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA *AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS* KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE QLENRDQMAPRAPRENQGKERWWITMETSMRLS RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD CQALQDTMGKRDLAVNQETWALLVPKAPQERMD LQE*REKTGTQGAQERRGKKGRQDKQAHRFLGC QGQRGLPDLRGSKVFLDQRGKQD*MEQKERKASR EKKETVVPWDYPELQVWTAGLGHRVLQDQLEFQ AQRDQRARGAAKETLG*QDQREQLGFLVYMDHP GTRETGGRGGRKALEGLKGIRETKERLD*MPPAH WAKMAYQSKAAGTSDASNLGLACVCVCT*NIYF YTVFTF*KCQKYDASYRLLKKKEKPAYFVQKIS TSSLLFTRCFV*AYVSNTFFVWS*CLHDICAHLL SIEA**IIVSWCQRGPARTEVLASSCVNSHKCR GP*YLLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP KQHASSSALQPNNCPEAAASPQFSATGSTPGHK KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT SRSEGRKRAPREPRREGGKRGDRTSRLTGSWAA RARGASRTSGAPRCSWTKGGSRTRWSKRRERLP GRKRRPWSPGTTRSFRFGRQAWATGYSRTNWSSR PSGTKGREGQQRRPWDDRTNGSSWASWFTWTTR GQGKPGGEGEERL*RA*RG*GRPRSAWIRCPLP IGRRWLTSPRLLEQVMPLTLDWPVCVFVHRIFIF IQFSLFENARSMMHLTDY*KRKKNLHILYRKYQ PLPFCLQDVLYKPMSLIHFLFGRNVCMIFVHIY *VSKLNKLLCPGAKGGQPELRCWLAHV*IHINVE VHDIC*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG PPGVKGENGHPGSPGEKGEKGETGQAGSPVPGLP GPEGPPGPPGLQGVPGPKGEAGLDGAKGEKGFQ GEKGDRGPLGLPGASGLDGRPGPPGTPGPIGVP GPAGPKGERGSKGDPGMTGPTGAAGLPGLHGPPG DKGNRGERGKKGSRGPKGDKGDQGAPGLDAPCP LGEDGLPVQGCWNK*CL*PWIGLCVCLYIEYLF LYSFHFLKMPEV*CILQIIKKERKTCIFCTENIN LFPFVYKMFCISLCL*YIFCLVVMSA*YLCTFI KYRSLINYCVLVPKGASQN*GAG*LMCEFT*M* RSMIFAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080805

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGG CCACCCAGGACCAAAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCA CACCGGGAGTGCCTAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCAC CCCCGGTCATAAAAAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCT GGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAG GGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAA CCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAG AGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAG GGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCA ATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAA GGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCA GGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGG GAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGT TCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCT TCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACC CTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAG AGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAG TGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCA TCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATG TCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAG GGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTT TAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ HFRVPLEDPDSQVNQELEVSLDFRVPLGWTANR ATQDQRATWV*RVPQDSRDPRDKKEKRVSVESTH TGSA*AACQQLCAPAK*LP*SCCLSSIQCDWLH PRS*KGGRSRANRARPASKVHQGPQAPLDQVDL WGTQDCQGLWGHLAYLGLLDQRETQGSRATTAGR ENGACQGCQASMEPRGRPELPWLG*RVSQGSQE PRVRRGLKAPLGFLASWGRRERKAMLATPLEEA EGNLALQGSLGPQGQREKQVSMARLAPQGSQETR GSVEQLENRDQMAPRAPRENQGKERWWITMETS MRLSRRSGRWP*WGLLVFLGKLAHLELQGFQAR RGRLDCQALQDTMGKRDLAVNQETWALLVPKAPQ ERMDLQE*REKTGTQGAQERRGKKGRQDKQAHR ERKEKPGRRAIQEQRFLGCQGQRGLPDLRGSKV FLDQRGKQD*MEQKERKASREKKETVVPWDYPEL QVWTAGLGHRVLQDQLEFQAQRDQRARGAAKET LG*QDQREQLGFLVYMDHPGTRETGGRGGRKAL EGLKGIRETKERLD*MPPAHWAKMAYQSKAAGTS DASNLGLACVCVCT*NIYFYTVFTF*KCQKYDA SYRLLKKKEKPAYFVQKISTSSLLFTRCFV*AY VSNTFFVWS*CLHDICAHLLSIEA**IIVSWCQR GPARTEVLASSCVNSHKCRGP*YLLS*VCLRVF *TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS ISGSHWKTRTPRSTRN*RFPWISGSHWAGRQTG PPRTKGRHGSDGSPRTAGTPGTKRRKGSVWRVP TPGVPKQHASSSALQPNNCPEAAASPQFSATGST PGHKKADVPGRTEPGQHPRSTRAPRPPWTKWTS GAPRTARAYGATWLTWASWTKGRPRDPGLPRPE GRTGHARDARQAWSQGGARNCRGWDEG*ARDPRN QG*EGG*RLPWASWPPGAEGRERRCWQLHWRRQ RGTWPSRAPWAPRAKGRSRCRWPGWPPRAARRQ GGAWSSWRTGTRWPQGLQGRTRERRDGGLQWKHQ *GSPGDPDAGLDGASWSSWANWPTWSSRDSRPE GGDWTARPSRTRWGKGTSR*TRRHGPSWSPRPP RKGWTSRSEGRKRAPREPRREGGKRGDRTSRLTG RERRSRGEGQSRSRGSWAARARGASRTSGAPRC SWTKGGSRTRWSKRRERLPGRKRRPWSPGTTRS FRFGRQAWATGYSRTNWSSRPSGTKGREGQQRRP WDDRTNGSSWASWFTWTTRGQGKPGGEGEERL* RA*RG*GRPRSAWIRCPLPIGRRWLTSPRLLEQ VMPLTLDWPVCVFVHRIFIFIQFSLFENARSMMH LTDY*KRKKNLHILYRKYQPLPFCLQDVLYKPM SLIHFLFGRNVCMIFVHIY*VSKLNKLLCPGAK GGQPELRCWLAHV*IHINVEVHDIC*ARCV*EYF KPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP AFQGPTGRPGLPGQPGTRGFPGFPGPIGLDGKPG HPGPKGDMGLTGPPGQPGPQGQKGEKGQCGEYP HRECLSSMPAALRSSQIIALKLLPLLNSVRLAP PPVIKRRTFQGEQSQASIQGPPGPPGPPGPSGPL GHPGLPGPMGPPGLPGPPGPKGDPGIQGYHGRK GERGMPGMPGKHGAKGAPGIAVAGMKGEPGIPG TKGEKGAEGSPGLPGLLGQKGEKGDAGNSIGGGR GEPGPPGLPGPPGPKGEAGVDGQVGPPGQPGDK GERGAAGEQGPDGPKGSKGEPGKGEMVDYNGNI NEALQEIRTLALMGPPGLPGQIGPPGAPGIPGQK GEIGLPGPPGHDGEKGPRGKPGDMGPPGPQGPP GKDGPPGVKGENGHPGSPGEKGEKGETGQAGSP GEKGEAGEKGNPGAEVPGLPGPEGPPGPPGLQGV PGPKGEAGLDGAKGEKGFQGEKGDRGPLGLPGA SGLDGRPGPPGTPGPIGVPGPAGPKGERGSKGD PGMTGPTGAAGLPGLHGPPGDKGNRGERGKKGSR GPKGDKGDQGAPGLDAPCPLGEDGLPVQGCWNK *CL*PWIGLCVCLYIEYLFLYSFHFLKMPEV*C ILQIIKKERKTCIFCTENINLFPFVYKMFCISLC L*YIFCLVVMSA*YLCTFIKYRSLINYCVLVPK GASQN*GAG*LMCEFT*M*RSMIFAKLGVSKSI LNPYGFSLLKEMKHGN

Sequence NM_080804

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGA AGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAG GGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGTACTCCAGGACCAATTG GAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGG ACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCC CCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAAT ATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGA AAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGC ACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACAT AAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA *AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS* KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE QLENRDQMAPRAPRENQGKERWWITMETSMRLS RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD CQALQDTMGKRDLAVNQETWALLVPKAPQERMD LQE*REKTGTQGAQERRGKKGRQDKQAHRERKE KPGRRAIQEQRFLGCQGQRGLPDLRGSKVFLDQR GKQD*MEQKERKASREKKETVVPWDYPVLQDQL EFQAQRDQRARGAAKETLG*QDQREQLGFLVYM DHPGTRETGGRGGRKALEGLKGIRETKERLD*MP PAHWAKMAYQSKAAGTSDASNLGLACVCVCT*N IYFYTVFTF*KCQKYDASYRLLKKKEKPAYFVQ KISTSSLLFTRCFV*AYVSNTFFVWS*CLHDICA HLLSIEA**IIVSWCQRGPARTEVLASSCVNSH KCRGP*YLLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP KQHASSSALQPNNCPEAAASPQFSATGSTPGHK KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT SRSEGRKRAPREPRREGGKRGDRTSRLTGRERR SRGEGQSRSRGSWAARARGASRTSGAPRCSWTK GGSRTRWSKRRERLPGRKRRPWSPGTTRYSRTNW SSRPSGTKGREGQQRRPWDDRTNGSSWASWFTW TTRGQGKPGGEGEERL*RA*RG*GRPRSAWIRC PLPIGRRWLTSPRLLEQVMPLTLDWPVCVFVHRI FIFIQFSLFENARSMMHLTDY*KRKKNLHILYR KYQPLPFCLQDVLYKPMSLIHFLFGRNVCMIFV HIY*VSKLNKLLCPGAKGGQPELRCWLAHV*IHI NVEVHDIC*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG PPGVKGENGHPGSPGEKGEKGETGQAGSPGEKGE AGEKGNPGAEVPGLPGPEGPPGPPGLQGVPGPK GEAGLDGAKGEKGFQGEKGDRGPLGLPGTPGPI GVPGPAGPKGERGSKGDPGMTGPTGAAGLPGLHG PPGDKGNRGERGKKGSRGPKGDKGDQGAPGLDA PCPLGEDGLPVQGCWNK*CL*PWIGLCVCLYIE YLFLYSFHFLKMPEV*CILQIIKKERKTCIFCTE NINLFPFVYKMFCISLCL*YIFCLVVMSA*YLC TFIKYRSLINYCVLVPKGASQN*GAG*LMCEFT *M*RSMIFAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080803

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGA AGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTC CAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTC CAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACC CGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGC CCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTAT TTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATAT CAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTT ATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGT AGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA *AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS* KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE QLENRDQMAPRAPRENQGKERWWITMETSMRLS RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD CQALQDTMGKRDLAVNQETWALLVPKAPQERMD LQE*REKTGTQGAQERRGKKGRQDKQAHRERKE KPGRRAIQEQRGSKVFLDQRGKQD*MEQKERKAS REKKETVVPWDYPELQVWTAGLGHRVLQDQLEF QAQRDQRARGAAKETLG*QDQREQLGFLVYMDH PGTRETGGRGGRKALEGLKGIRETKERLD*MPPA HWAKMAYQSKAAGTSDASNLGLACVCVCT*NIY FYTVFTF*KCQKYDASYRLLKKKEKPAYFVQKI STSSLLFTRCFV*AYVSNTFFVWS*CLHDICAHL LSIEA**IIVSWCQRGPARTEVLASSCVNSHKC RGP*YLLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP KQHASSSALQPNNCPEAAASPQFSATGSTPGHK KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT SRSEGRKRAPREPRREGGKRGDRTSRLTGRERR SRGEGQSRSRGAPRCSWTKGGSRTRWSKRRERL PGRKRRPWSPGTTRSFRFGRQAWATGYSRTNWSS RPSGTKGREGQQRRPWDDRTNGSSWASWFTWTT RGQGKPGGEGEERL*RA*RG*GRPRSAWIRCPL PIGRRWLTSPRLLEQVMPLTLDWPVCVFVHRIFI FIQFSLFENARSMMHLTDY*KRKKNLHILYRKY QPLPFCLQDVLYKPMSLIHFLFGRNVCMIFVHI Y*VSKLNKLLCPGAKGGQPELRCWLAHV*IHINV EVHDIC*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG PPGVKGENGHPGSPGEKGEKGETGQAGSPGEKGE AGEKGNPGAEGLQGVPGPKGEAGLDGAKGEKGF QGEKGDRGPLGLPGASGLDGRPGPPGTPGPIGV PGPAGPKGERGSKGDPGMTGPTGAAGLPGLHGPP GDKGNRGERGKKGSRGPKGDKGDQGAPGLDAPC PLGEDGLPVQGCWNK*CL*PWIGLCVCLYIEYL FLYSFHFLKMPEV*CILQIIKKERKTCIFCTENI NLFPFVYKMFCISLCL*YIFCLVVMSA*YLCTF IKYRSLINYCVLVPKGASQN*GAG*LMCEFT*M *RSMIFAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080807

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGCTGCTGC CTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAAAAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCC CCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGAC CCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTG GGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGG CGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAG GTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAG GAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCC ACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGAC ATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAA AAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGG GCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGA GACCGTGGTCCCCTGGGACTACCCGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGA TGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCC TAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGC CTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTAC AGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTA ATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCC AGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACC CTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ RATWV*RVPQDSRDPRDKKEKRVSVESTHTGCC LSSIQCDWLHPRS*KGGRSRANRARPASKVHQG PQAPLDQVDLWGTQDCQGLWGHLAYLGLLDQRET QGSRATTAGRENGACQGCQASMEPRGRPELPWL G*RVSQGSQEPRVRRGLKAPLGFLASWGRRERK AMLATPLEEAEGNLALQGSLGPQGQREKQVSMAR LAPQGSQETRGSVEQLENRDQMAPRAPRENQGK ERWWITMETSMRLSRRSGRWP*WGLLVFLGKLA HLELQGFQARRGRLDCQALQDTMGKRDLAVNQET WALLVPKAPQERMDLQE*REKTGTQGAQERRGK KGRQDKQAHRERKEKPGRRAIQEQRFLGCQGQR GLPDLRGSKVFLDQRGKQD*MEQKERKASREKKE TVVPWDYPVLQDQLEFQAQRDQRARGAAKETLG *QDQREQLGFLVYMDHPGTRETGGRGGRKALEG LKGIRETKERLD*MPPAHWAKMAYQSKAAGTSDA SNLGLACVCVCT*NIYFYTVFTF*KCQKYDASY RLLKKKEKPAYFVQKISTSSLLFTRCFV*AYVS NTFFVWS*CLHDICAHLLSIEA**IIVSWCQRGP ARTEVLASSCVNSHKCRGP*YLLS*VCLRVF*T LMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPAAA SPQFSATGSTPGHKKADVPGRTEPGQHPRSTRA PRPPWTKWTSGAPRTARAYGATWLTWASWTKGR PRDPGLPRPEGRTGHARDARQAWSQGGARNCRGW DEG*ARDPRNQG*EGG*RLPWASWPPGAEGRER RCWQLHWRRQRGTWPSRAPWAPRAKGRSRCRWP GWPPRAARRQGGAWSSWRTGTRWPQGLQGRTRER RDGGLQWKHQ*GSPGDPDAGLDGASWSSWANWP TWSSRDSRPEGGDWTARPSRTRWGKGTSR*TRR HGPSWSPRPPRKGWTSRSEGRKRAPREPRREGGK RGDRTSRLTGRERRSRGEGQSRSRGSWAARARG ASRTSGAPRCSWTKGGSRTRWSKRRERLPGRKR RPWSPGTTRYSRTNWSSRPSGTKGREGQQRRPWD DRTNGSSWASWFTWTTRGQGKPGGEGEERL*RA *RG*GRPRSAWIRCPLPIGRRWLTSPRLLEQVM PLTLDWPVCVFVHRIFIFIQFSLFENARSMMHLT DY*KRKKNLHILYRKYQPLPFCLQDVLYKPMSL IHFLFGRNVCMIFVHIY*VSKLNKLLCPGAKGG QPELRCWLAHV*IHINVEVHDIC*ARCV*EYFKP LWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHRLL PLLNSVRLAPPPVIKRRTFQGEQSQASIQGPPGP PGPPGPSGPLGHPGLPGPMGPPGLPGPPGPKGD PGIQGYHGRKGERGMPGMPGKHGAKGAPGIAVA GMKGEPGIPGTKGEKGAEGSPGLPGLLGQKGEKG DAGNSIGGGRGEPGPPGLPGPPGPKGEAGVDGQ VGPPGQPGDKGERGAAGEQGPDGPKGSKGEPGK GEMVDYNGNINEALQEIRTLALMGPPGLPGQIGP PGAPGIPGQKGEIGLPGPPGHDGEKGPRGKPGD MGPPGPQGPPGKDGPPGVKGENGHPGSPGEKGE KGETGQAGSPGEKGEAGEKGNPGAEVPGLPGPEG PPGPPGLQGVPGPKGEAGLDGAKGEKGFQGEKG DRGPLGLPGTPGPIGVPGPAGPKGERGSKGDPG MTGPTGAAGLPGLHGPPGDKGNRGERGKKGSRGP KGDKGDQGAPGLDAPCPLGEDGLPVQGCWNK*C L*PWIGLCVCLYIEYLFLYSFHFLKMPEV*CIL QIIKKERKTCIFCTENINLFPFVYKMFCISLCL* YIFCLVVMSA*YLCTFIKYRSLINYCVLVPKGA SQN*GAG*LMCEFT*M*RSMIFAKLGVSKSILN PYGFSLLKEMKHGN

Sequence NM_080814

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG CCCCGCAGGGGTCCCCCGGAGACGCTGGGCTGTCCATCATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCC CATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCAAAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAA AAGGGTCAGTGTGGAGAGTACCCACACCGGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTC TGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAA GGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGA ACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCA GAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAA GGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATC AATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGA AGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCC AGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCG GGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTG TTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGC TTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGAC CCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTA GAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAA GTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGC ATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTAT GTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAA GGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATT TTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC

refseq_COL13A1.F1 (forward primer)
refseq_COL13A1.R1 (reverse primer)
Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ PRRGPPETLGCPSLVPAAPLVNQELEVSLDFRV PLGWTANRATQDQRATWV*RVPQDSRDPRDKKEK RVSVESTHTGANRARPASKVHQGPQAPLDQVDL WGTQDCQGLWGHLAYLGLLDQRETQGSRATTAG RENGACQGCQASMEPRGRPELPWLG*RVSQGSQE PRVRRGLKAPLGFLASWGRRERKAMLATPLEEA EGNLALQGSLGPQGQREKQVSMARLAPQGSQET RGSVEQLENRDQMAPRAPRENQGKERWWITMETS MRLSRRSGRWP*WGLLVFLGKLAHLELQGFQAR RGRLDCQALQDTMGKRDLAVNQETWALLVPKAP QERMDLQE*REKTGTQGAQERRGKKGRQDKQAHR ERKEKPGRRAIQEQRFLGCQGQRGLPDLRGSKV FLDQRGKQD*MEQKERKASREKKETVVPWDYPE LQVWTAGLGHRVLQDQLEFQAQRDQRARGAAKET LG*QDQREQLGFLVYMDHPGTRETGGRGGRKAL EGLKGIRETKERLD*MPPAHWAKMAYQSKAAGT SDASNLGLACVCVCT*NIYFYTVFTF*KCQKYDA SYRLLKKKEKPAYFVQKISTSSLLFTRCFV*AY VSNTFFVWS*CLHDICAHLLSIEA**IIVSWCQ RGPARTEVLASSCVNSHKCRGP*YLLS*VCLRVF *TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS PAGVPRRRWAVHHWSPRPPWSTRN*RFPWISGS HWAGRQTGPPRTKGRHGSDGSPRTAGTPGTKRR KGSVWRVPTPGRTEPGQHPRSTRAPRPPWTKWTS GAPRTARAYGATWLTWASWTKGRPRDPGLPRPE GRTGHARDARQAWSQGGARNCRGWDEG*ARDPR NQG*EGG*RLPWASWPPGAEGRERRCWQLHWRRQ RGTWPSRAPWAPRAKGRSRCRWPGWPPRAARRQ GGAWSSWRTGTRWPQGLQGRTRERRDGGLQWKH Q*GSPGDPDAGLDGASWSSWANWPTWSSRDSRPE GGDWTARPSRTRWGKGTSR*TRRHGPSWSPRPP RKGWTSRSEGRKRAPREPRREGGKRGDRTSRLT GRERRSRGEGQSRSRGSWAARARGASRTSGAPRC SWTKGGSRTRWSKRRERLPGRKRRPWSPGTTRS FRFGRQAWATGYSRTNWSSRPSGTKGREGQQRR PWDDRTNGSSWASWFTWTTRGQGKPGGEGEERL* RA*RG*GRPRSAWIRCPLPIGRRWLTSPRLLEQ VMPLTLDWPVCVFVHRIFIFIQFSLFENARSMM HLTDY*KRKKNLHILYRKYQPLPFCLQDVLYKPM SLIHFLFGRNVCMIFVHIY*VSKLNKLLCPGAK GGQPELRCWLAHV*IHINVEVHDIC*ARCV*EY FKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF YLFIYWFSRRERMVAERTHKAAATGARGPGELG APGTVALVAARAERGARLPSPGSCGLLTLALCSL ALSLLAHFRTAELQARVLRLEAERGEQQMETAI LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP APQGSPGDAGLSIIGPRGPPGQPGTRGFPGFPGP IGLDGKPGHPGPKGDMGLTGPPGQPGPQGQKGE KGQCGEYPHRGEQSQASIQGPPGPPGPPGPSGP LGHPGLPGPMGPPGLPGPPGPKGDPGIQGYHGRK GERGMPGMPGKHGAKGAPGIAVAGMKGEPGIPG TKGEKGAEGSPGLPGLLGQKGEKGDAGNSIGGG RGEPGPPGLPGPPGPKGEAGVDGQVGPPGQPGDK GERGAAGEQGPDGPKGSKGEPGKGEMVDYNGNI NEALQEIRTLALMGPPGLPGQIGPPGAPGIPGQ KGEIGLPGPPGHDGEKGPRGKPGDMGPPGPQGPP GKDGPPGVKGENGHPGSPGEKGEKGETGQAGSP GEKGEAGEKGNPGAEVPGLPGPEGPPGPPGLQG VPGPKGEAGLDGAKGEKGFQGEKGDRGPLGLPGA SGLDGRPGPPGTPGPIGVPGPAGPKGERGSKGD PGMTGPTGAAGLPGLHGPPGDKGNRGERGKKGS RGPKGDKGDQGAPGLDAPCPLGEDGLPVQGCWNK *CL*PWIGLCVCLYIEYLFLYSFHFLKMPEV*C ILQIIKKERKTCIFCTENINLFPFVYKMFCISL CL*YIFCLVVMSA*YLCTFIKYRSLINYCVLVPK GASQN*GAG*LMCEFT*M*RSMIFAKLGVSKSI LNPYGFSLLKEMKHGN