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Sequences

Sequence NM_181359

GGCGGTCCCCTGTTCTCCCCGCTCAGGTGCGGCGCTGTGGCAGGAAGCCACCCCCTCGGTCGGCCGGTGCGCGGGGCTGTTGCGCCATCCGCTCCGGCTT
TCGTAACCGCACCCTGGGACGGCCCAGAGACGCTCCAGCGCGAGTTCCTCAAATGTTTTCCTGCGTTGCCAGGACCGTCCGCCGCTCTGAGTCATGTGCG
AGTGGGAAGTCGCACTGACACTGAGCCGGGCCAGAGGGAGAGGAGCCGAGCGCGGCGCGGGGCCGAGGGACTCGCAGTGTGTGTAGAGAGCCGGGCTCCT
GCGGATGGGGGCTGCCCCCGGGGCCTGAGCCCGCCTGCCCGCCCACCGCCCCGCCCCGCCCCTGCCACCCCTGCCGCCCGGTTCCCATTAGCCTGTCCGC
CTCTGCGGGACCATGGAGTGGTAGCCGAGGAGGAAGCATGCTGGCCGTCGGCTGCGCGCTGCTGGCTGCCCTGCTGGCCGCGCCGGGAGCGGCGCTGGCC
CCAAGGCGCTGCCCTGCGCAGGAGGTGGCGAGAGGCGTGCTGACCAGTCTGCCAGGAGACAGCGTGACTCTGACCTGCCCGGGGGTAGAGCCGGAAGACA
ATGCCACTGTTCACTGGGTGCTCAGGAAGCCGGCTGCAGGCTCCCACCCCAGCAGATGGGCTGGCATGGGAAGGAGGCTGCTGCTGAGGTCGGTGCAGCT
CCACGACTCTGGAAACTATTCATGCTACCGGGCCGGCCGCCCAGCTGGGACTGTGCACTTGCTGGTGGATGTTCCCCCCGAGGAGCCCCAGCTCTCCTGC
TTCCGGAAGAGCCCCCTCAGCAATGTTGTTTGTGAGTGGGGTCCTCGGAGCACCCCATCCCTGACGACAAAGGCTGTGCTCTTGGTGAGGAAGTTTCAGA
ACAGTCCGGCCGAAGACTTCCAGGAGCCGTGCCAGTATTCCCAGGAGTCCCAGAAGTTCTCCTGCCAGTTAGCAGTCCCGGAGGGAGACAGCTCTTTCTA
CATAGTGTCCATGTGCGTCGCCAGTAGTGTCGGGAGCAAGTTCAGCAAAACTCAAACCTTTCAGGGTTGTGGAATCTTGCAGCCTGATCCGCCTGCCAAC
ATCACAGTCACTGCCGTGGCCAGAAACCCCCGCTGGCTCAGTGTCACCTGGCAAGACCCCCACTCCTGGAACTCATCTTTCTACAGACTACGGTTTGAGC
TCAGATATCGGGCTGAACGGTCAAAGACATTCACAACATGGATGGTCAAGGACCTCCAGCATCACTGTGTCATCCACGACGCCTGGAGCGGCCTGAGGCA
CGTGGTGCAGCTTCGTGCCCAGGAGGAGTTCGGGCAAGGCGAGTGGAGCGAGTGGAGCCCGGAGGCCATGGGCACGCCTTGGACAGAATCCAGGAGTCCT
CCAGCTGAGAACGAGGTGTCCACCCCCATGCAGGCACTTACTACTAATAAAGACGATGATAATATTCTCTTCAGAGATTCTGCAAATGCGACAAGCCTCC
CAGGTTCAAGAAGACGTGGAAGCTGCGGGCTCTGAAGGAAGGCAAGACAAGCATGCATCCGCCGTACTCTTTGGGGCAGCTGGTCCCGGAGAGGCCTCGA
CCCACCCCAGTGCTTGTTCCTCTCATCTCCCCACCGGTGTCCCCCAGCAGCCTGGGGTCTGACAATACCTCGAGCCACAACCGACCAGATGCCAGGGACC
CACGGAGCCCTTATGACATCAGCAATACAGACTACTTCTTCCCCAGATAGCTGGCTGGGTGGCACCAGCAGCCTGGACCCTGTGGATGATAAAACACAAA
CGGGCTCAGCAAAAGATGCTTCTCACTGCCATGCCAGCTTATCTCAGGGGTGTGCGGCCTTTGGCTTCACGGAAGAGCCTTGCGGAAGGTTCTACGCCAG
GGGAAAATCAGCCTGCTCCAGCTGTTCAGCTGGTTGAGGTTTCAAACCTCCCTTTCCAAATGCCCAGCTTAAAGGGGCTAGAGTGAACTTGGGCCACTGT
GAAGAGAACCATATCAAGACTCTTTGGACACTCACACGGACACTCAAAAGCTGGGCAGGTTGGTGGGGGCCTCGGTGTGGAGAAGCGGCTGGCAGCCCAC
CCCTCAACACCTCTGCACAAGCTGCACCCTCAGGCAGGTGGGATGGATTTCCAGCCAAAGCCTCCTCCAGCCGCCATGCTCCTGGCCCACTGCATCGTTT
CATCTTCCAACTCAAACTCTTAAAACCCAAGTGCCTTAGCAAATTCTGTTTTTCTAGGCCTGGGGACGGCTTTTACTTAAACCGCCAAGGCTGGGGGAAG
AAGCTCTCTCCTCCCTTTCTTCCCTACAGTTGAAAAACAGCTGAGGGTGAGTGGGTGAATAATACAGTATCTCAGGGCCTGGTCGTTTTCAACAGAATTA
TAATTAGTTCCTCATTAGCATTTTGCTAAATGTGAATGATGATCCTAGGCATTTGCTGAATACAGAGGCAACTGCATTGGCTTTGGGTTGCAGGACCTCA
GGTGAGAAGCAGAGGAAGGAGAGGAGAGGGGCACAGGGTCTCTACCATCCCCTGTAGAGTGGGAGCTGAGTGGGGGATCACAGCCTCTGAAAACCAATGT
TCTCTCTTCTCCACCTCCCACAAAGGAGAGCTAGCAGCAGGGAGGGCTTCTGCCATTTCTGAGATCAAAACGGTTTTACTGCAGCTTTGTTTGTTGTCAG
CTGAACCTGGGTAACTAGGGAAGATAATATTAAGGAAGACAATGTGAAAAGAAAAATGAGCCTGGCAAGAATGTGTTTAAACTTGGTTTTTAAAAAACTG
CTGACTGTTTTCTCTTGAGAGGGTGGAATATCCAATATTCGCTGTGTCAGCATAGAAGTAACTTACTTAGGTGTGGGGGAAGCACCATAACTTTGTTTAG
CCCAAAACCAAGTCAAGTGAAAAAGGAGGAAGAGAAAAAATATTTTCCTGCCAGGCATGGTGGCCCACGCACTTCGGGAGGTCGAGGCAGGAGGATCACT
TGAGTCCAGAAGTTTGAGATCAGCCTGGGCAATGTGATAAAACCCCATCTCTACAAAAAGCATAAAAATTAGCCAAGTGTGGTAGAGTGTGCCTGAAGTC
CCAGATACTTGGGGGGCTGAGGTGGGAGGATCTCTTGAGCCTGGGAGGTCAAGGCTGCAGTGAGCCGAGATTGCACCACTGCACTCCAGCCTGGGTGACA
GAGCAAGTGAGACCCTGTCTCAAAAAAAGAAAAAGAAAAAGAAAAAATATTTTCCCTATTAGAGAAGAGATTGTGGTTTCATTCTGTATTTTGTTTTTGT
CTTAAAAAGTGGAAAAATAGCCTGCCTCTTCTCTACTCTAGGGAAAAACCAGCGTGTGACTACTCCCCCAGGTGGTTATGGAGAGGGTGTCCGGTCCCTG
TCCCAGTGCCGAGAAGGAAGCCTCCCACGACTGCCCGGCAGGGTCCTAGAAATTCCCCACCCTGAAAGCCCTGAGCTTTCTGCTATCAAAGAGGTTTTAA
AAAAATCCCATTTAAAAAAAATCCCTTACCTCGGTGCCTTCCTCTTTTTATTTAGTTCCTTGAGTTGATTCAGCTCTGCAAGAATTGAAGCAGGACTAAA
TGTCTAGTTGTAACACCATGATTAACCACTTCAGCTGACTTTTCTGTCCGAGCTTTGAAAATTCAGTGGTGTTAGTGGTTACCCAGTTAGCTCTCAAGTT
ATCAGGGTATTCCAGAGTGGGGATATGATTTAAATCAGCCGTGTAACCATGGACCCAATATTTACCAGACCACAAAACTTTTCTAATACTCTACCCTCTT
AGAAAAACCACCACCATCACCAGACAGGTGCGAAAGGATGAAAGTGACCATGTTTTGTTTACGGTTTTCCAGGTTTAAGCTGTTACTGTCTTCAGTAAGC
CGTGATTTTCATTGCTGGGCTTGTCTGTAGATTTTAGACCCTATTGCTGCTTGAGGCAACTCATCTTAGGTTGGCAAAAAGGCAGGATGGCCGGGCGCGG
TGGCTCACGCCTGTAATCCTAGCACTTTGGGAGGCCAAGGTGGGAGGATTGCTTGAGCTCAGGAGTTTGAGACCAACCTGGG
  • refseq_IL6R.F2 (forward primer)
  • refseq_IL6R.R2 (reverse primer)
  • Single exon skipping

Frame 0

GGPLFSPLRCGAVAGSHPLGRPVRGAVAPSAPA
FVTAPWDGPETLQREFLKCFPALPGPSAALSHVR
VGSRTDTEPGQRERSRARRGAEGLAVCVESRAP
ADGGCPRGLSPPARPPPRPAPATPAARFPLACP
PLRDHGVVAEEEACWPSAARCWLPCWPRRERRWP
QGAALRRRWREAC*PVCQETA*L*PARG*SRKT
MPLFTGCSGSRLQAPTPADGLAWEGGCC*GRCS
STTLETIHATGPAAQLGLCTCWWMFPPRSPSSPA
SGRAPSAMLFVSGVLGAPHP*RQRLCSW*GSFR
TVRPKTSRSRASIPRSPRSSPAS*QSRRETALS
T*CPCASPVVSGASSAKLKPFRVVESCSLIRLPT
SQSLPWPETPAGSVSPGKTPTPGTHLSTDYGLS
SDIGLNGQRHSQHGWSRTSSITVSSTTPGAA*G
TWCSFVPRRSSGKASGASGARRPWARLGQNPGVL
QLRTRCPPPCRHLLLIKTMIIFSSEILQMRQAS
QVQEDVEAAGSEGRQDKHASAVLFGAAGPGEAS
THPSACSSHLPTGVPQQPGV*QYLEPQPTRCQGP
TEPL*HQQYRLLLPQIAGWVAPAAWTLWMIKHK
RAQQKMLLTAMPAYLRGVRPLASRKSLAEGSTP
GENQPAPAVQLVEVSNLPFQMPSLKGLE*TWATV
KRTISRLFGHSHGHSKAGQVGGGLGVEKRLAAH
PSTPLHKLHPQAGGMDFQPKPPPAAMLLAHCIV
SSSNSNS*NPSALANSVFLGLGTAFT*TAKAGGR
SSLLPFFPTVEKQLRVSG*IIQYLRAWSFSTEL
*LVPH*HFAKCE**S*AFAEYRGNCIGFGLQDL
R*EAEEGEERGTGSLPSPVEWELSGGSQPLKTNV
LSSPPPTKES*QQGGLLPFLRSKRFYCSFVCCQ
LNLGN*GR*Y*GRQCEKKNEPGKNVFKLGF*KT
ADCFLLRGWNIQYSLCQHRSNLLRCGGSTITLFS
PKPSQVKKEEEKKYFPARHGGPRTSGGRGRRIT
*VQKFEISLGNVIKPHLYKKHKN*PSVVECA*S
PRYLGG*GGRIS*AWEVKAAVSRDCTTALQPG*Q
SK*DPVSKKEKEKEKIFSLLEKRLWFHSVFCFC
LKKWKNSLPLLYSREKPACDYSPRWLWRGCPVP
VPVPRRKPPTTARQGPRNSPP*KP*AFCYQRGFK
KIPFKKNPLPRCLPLFI*FLELIQLCKN*SRTK
CLVVTP*LTTSADFSVRALKIQWC*WLPS*LSS
YQGIPEWGYDLNQPCNHGPNIYQTTKLF*YSTLL
EKPPPSPDRCERMKVTMFCLRFSRFKLLLSSVS
RDFHCWACL*ILDPIAA*GNSS*VGKKAGWPGA
VAHACNPSTLGGQGGRIA*AQEFETNL

Frame 1

AVPCSPRSGAALWQEATPSVGRCAGLLRHPLRL
S*PHPGTAQRRSSASSSNVFLRCQDRPPL*VMC
EWEVALTLSRARGRGAERGAGPRDSQCV*RAGLL
RMGAAPGA*ARLPAHRPAPPLPPLPPGSH*PVR
LCGTMEW*PRRKHAGRRLRAAGCPAGRAGSGAG
PKALPCAGGGERRADQSARRQRDSDLPGGRAGRQ
CHCSLGAQEAGCRLPPQQMGWHGKEAAAEVGAA
PRLWKLFMLPGRPPSWDCALAGGCSPRGAPALL
LPEEPPQQCCL*VGSSEHPIPDDKGCALGEEVSE
QSGRRLPGAVPVFPGVPEVLLPVSSPGGRQLFL
HSVHVRRQ*CREQVQQNSNLSGLWNLAA*SACQ
HHSHCRGQKPPLAQCHLARPPLLELIFLQTTV*A
QISG*TVKDIHNMDGQGPPASLCHPRRLERPEA
RGAASCPGGVRARRVERVEPGGHGHALDRIQES
SS*ERGVHPHAGTYY**RR**YSLQRFCKCDKPP
RFKKTWKLRALKEGKTSMHPPYSLGQLVPERPR
PTPVLVPLISPPVSPSSLGSDNTSSHNRPDARD
PRSPYDISNTDYFFPR*LAGWHQQPGPCG**NTN
GLSKRCFSLPCQLISGVCGLWLHGRALRKVLRQ
GKISLLQLFSWLRFQTSLSKCPA*RG*SELGPL
*REPYQDSLDTHTDTQKLGRLVGASVWRSGWQPT
PQHLCTSCTLRQVGWISSQSLLQPPCSWPTASF
HLPTQTLKTQVP*QILFF*AWGRLLLKPPRLGE
EALSSLSSLQLKNS*G*VGE*YSISGPGRFQQNY
N*FLISILLNVNDDPRHLLNTEATALALGCRTS
GEKQRKERRGAQGLYHPL*SGS*VGDHSL*KPM
FSLLHLPQRRASSREGFCHF*DQNGFTAALFVVS
*TWVTREDNIKEDNVKRKMSLARMCLNLVFKKL
LTVFS*EGGISNIRCVSIEVTYLGVGEAP*LCL
AQNQVK*KRRKRKNIFLPGMVAHALREVEAGGSL
ESRSLRSAWAM**NPISTKSIKISQVW*SVPEV
PDTWGAEVGGSLEPGRSRLQ*AEIAPLHSSLGD
RASETLSQKKKKKKKKYFPY*RRDCGFILYFVFV
LKSGKIACLFSTLGKNQRVTTPPGGYGEGVRSL
SQCREGSLPRLPGRVLEIPHPESPELSAIKEVL
KKSHLKKIPYLGAFLFLFSSLS*FSSARIEAGLN
V*L*HHD*PLQLTFLSEL*KFSGVSGYPVSSQV
IRVFQSGDMI*ISRVTMDPIFTRPQNFSNTLPS
*KNHHHHQTGAKG*K*PCFVYGFPGLSCYCLQ*A
VIFIAGLVCRF*TLLLLEATHLRLAKRQDGRAR
WLTPVILALWEAKVGGLLELRSLRPTW

Frame 2

RSPVLPAQVRRCGRKPPPRSAGARGCCAIRSGF
RNRTLGRPRDAPARVPQMFSCVARTVRRSESCA
SGKSH*H*AGPEGEEPSAARGRGTRSVCREPGS
CGWGLPPGPEPACPPTAPPRPCHPCRPVPISLSA
SAGPWSGSRGGSMLAVGCALLAALLAAPGAALA
PRRCPAQEVARGVLTSLPGDSVTLTCPGVEPED
NATVHWVLRKPAAGSHPSRWAGMGRRLLLRSVQL
HDSGNYSCYRAGRPAGTVHLLVDVPPEEPQLSC
FRKSPLSNVVCEWGPRSTPSLTTKAVLLVRKFQ
NSPAEDFQEPCQYSQESQKFSCQLAVPEGDSSFY
IVSMCVASSVGSKFSKTQTFQGCGILQPDPPAN
ITVTAVARNPRWLSVTWQDPHSWNSSFYRLRFE
LRYRAERSKTFTTWMVKDLQHHCVIHDAWSGLRH
VVQLRAQEEFGQGEWSEWSPEAMGTPWTESRSP
PAENEVSTPMQALTTNKDDDNILFRDSANATSL
PGSRRRGSCGL*RKARQACIRRTLWGSWSRRGLD
PPQCLFLSSPHRCPPAAWGLTIPRATTDQMPGT
HGALMTSAIQTTSSPDSWLGGTSSLDPVDDKTQ
TGSAKDASHCHASLSQGCAAFGFTEEPCGRFYAR
GKSACSSCSAG*GFKPPFPNAQLKGARVNLGHC
EENHIKTLWTLTRTLKSWAGWWGPRCGEAAGSP
PLNTSAQAAPSGRWDGFPAKASSSRHAPGPLHRF
IFQLKLLKPKCLSKFCFSRPGDGFYLNRQGWGK
KLSPPFLPYS*KTAEGEWVNNTVSQGLVVFNRI
IISSSLAFC*M*MMILGIC*IQRQLHWLWVAGPQ
VRSRGRRGEGHRVSTIPCRVGAEWGITASENQC
SLFSTSHKGELAAGRASAISEIKTVLLQLCLLS
AEPG*LGKIILRKTM*KEK*AWQECV*TWFLKNC
*LFSLERVEYPIFAVSA*K*LT*VWGKHHNFV*
PKTKSSEKGGREKIFSCQAWWPTHFGRSRQEDH
LSPEV*DQPGQCDKTPSLQKA*KLAKCGRVCLKS
QILGGLRWEDLLSLGGQGCSEPRLHHCTPAWVT
EQVRPCLKKRKRKRKNIFPIREEIVVSFCILFL
S*KVEK*PASSLL*GKTSV*LLPQVVMERVSGPC
PSAEKEASHDCPAGS*KFPTLKALSFLLSKRF*
KNPI*KKSLTSVPSSFYLVP*VDSALQELKQD*
MSSCNTMINHFS*LFCPSFENSVVLVVTQLALKL
SGYSRVGI*FKSAV*PWTQYLPDHKTFLILYPL
RKTTTITRQVRKDESDHVLFTVFQV*AVTVFSK
P*FSLLGLSVDFRPYCCLRQLILGWQKGRMAGRG
GSRL*S*HFGRPRWEDCLSSGV*DQPG

Sequence NM_000565

GGCGGTCCCCTGTTCTCCCCGCTCAGGTGCGGCGCTGTGGCAGGAAGCCACCCCCTCGGTCGGCCGGTGCGCGGGGCTGTTGCGCCATCCGCTCCGGCTT
TCGTAACCGCACCCTGGGACGGCCCAGAGACGCTCCAGCGCGAGTTCCTCAAATGTTTTCCTGCGTTGCCAGGACCGTCCGCCGCTCTGAGTCATGTGCG
AGTGGGAAGTCGCACTGACACTGAGCCGGGCCAGAGGGAGAGGAGCCGAGCGCGGCGCGGGGCCGAGGGACTCGCAGTGTGTGTAGAGAGCCGGGCTCCT
GCGGATGGGGGCTGCCCCCGGGGCCTGAGCCCGCCTGCCCGCCCACCGCCCCGCCCCGCCCCTGCCACCCCTGCCGCCCGGTTCCCATTAGCCTGTCCGC
CTCTGCGGGACCATGGAGTGGTAGCCGAGGAGGAAGCATGCTGGCCGTCGGCTGCGCGCTGCTGGCTGCCCTGCTGGCCGCGCCGGGAGCGGCGCTGGCC
CCAAGGCGCTGCCCTGCGCAGGAGGTGGCGAGAGGCGTGCTGACCAGTCTGCCAGGAGACAGCGTGACTCTGACCTGCCCGGGGGTAGAGCCGGAAGACA
ATGCCACTGTTCACTGGGTGCTCAGGAAGCCGGCTGCAGGCTCCCACCCCAGCAGATGGGCTGGCATGGGAAGGAGGCTGCTGCTGAGGTCGGTGCAGCT
CCACGACTCTGGAAACTATTCATGCTACCGGGCCGGCCGCCCAGCTGGGACTGTGCACTTGCTGGTGGATGTTCCCCCCGAGGAGCCCCAGCTCTCCTGC
TTCCGGAAGAGCCCCCTCAGCAATGTTGTTTGTGAGTGGGGTCCTCGGAGCACCCCATCCCTGACGACAAAGGCTGTGCTCTTGGTGAGGAAGTTTCAGA
ACAGTCCGGCCGAAGACTTCCAGGAGCCGTGCCAGTATTCCCAGGAGTCCCAGAAGTTCTCCTGCCAGTTAGCAGTCCCGGAGGGAGACAGCTCTTTCTA
CATAGTGTCCATGTGCGTCGCCAGTAGTGTCGGGAGCAAGTTCAGCAAAACTCAAACCTTTCAGGGTTGTGGAATCTTGCAGCCTGATCCGCCTGCCAAC
ATCACAGTCACTGCCGTGGCCAGAAACCCCCGCTGGCTCAGTGTCACCTGGCAAGACCCCCACTCCTGGAACTCATCTTTCTACAGACTACGGTTTGAGC
TCAGATATCGGGCTGAACGGTCAAAGACATTCACAACATGGATGGTCAAGGACCTCCAGCATCACTGTGTCATCCACGACGCCTGGAGCGGCCTGAGGCA
CGTGGTGCAGCTTCGTGCCCAGGAGGAGTTCGGGCAAGGCGAGTGGAGCGAGTGGAGCCCGGAGGCCATGGGCACGCCTTGGACAGAATCCAGGAGTCCT
CCAGCTGAGAACGAGGTGTCCACCCCCATGCAGGCACTTACTACTAATAAAGACGATGATAATATTCTCTTCAGAGATTCTGCAAATGCGACAAGCCTCC
CAGTGCAAGATTCTTCTTCAGTACCACTGCCCACATTCCTGGTTGCTGGAGGGAGCCTGGCCTTCGGAACGCTCCTCTGCATTGCCATTGTTCTGAGGTT
CAAGAAGACGTGGAAGCTGCGGGCTCTGAAGGAAGGCAAGACAAGCATGCATCCGCCGTACTCTTTGGGGCAGCTGGTCCCGGAGAGGCCTCGACCCACC
CCAGTGCTTGTTCCTCTCATCTCCCCACCGGTGTCCCCCAGCAGCCTGGGGTCTGACAATACCTCGAGCCACAACCGACCAGATGCCAGGGACCCACGGA
GCCCTTATGACATCAGCAATACAGACTACTTCTTCCCCAGATAGCTGGCTGGGTGGCACCAGCAGCCTGGACCCTGTGGATGATAAAACACAAACGGGCT
CAGCAAAAGATGCTTCTCACTGCCATGCCAGCTTATCTCAGGGGTGTGCGGCCTTTGGCTTCACGGAAGAGCCTTGCGGAAGGTTCTACGCCAGGGGAAA
ATCAGCCTGCTCCAGCTGTTCAGCTGGTTGAGGTTTCAAACCTCCCTTTCCAAATGCCCAGCTTAAAGGGGCTAGAGTGAACTTGGGCCACTGTGAAGAG
AACCATATCAAGACTCTTTGGACACTCACACGGACACTCAAAAGCTGGGCAGGTTGGTGGGGGCCTCGGTGTGGAGAAGCGGCTGGCAGCCCACCCCTCA
ACACCTCTGCACAAGCTGCACCCTCAGGCAGGTGGGATGGATTTCCAGCCAAAGCCTCCTCCAGCCGCCATGCTCCTGGCCCACTGCATCGTTTCATCTT
CCAACTCAAACTCTTAAAACCCAAGTGCCTTAGCAAATTCTGTTTTTCTAGGCCTGGGGACGGCTTTTACTTAAACCGCCAAGGCTGGGGGAAGAAGCTC
TCTCCTCCCTTTCTTCCCTACAGTTGAAAAACAGCTGAGGGTGAGTGGGTGAATAATACAGTATCTCAGGGCCTGGTCGTTTTCAACAGAATTATAATTA
GTTCCTCATTAGCATTTTGCTAAATGTGAATGATGATCCTAGGCATTTGCTGAATACAGAGGCAACTGCATTGGCTTTGGGTTGCAGGACCTCAGGTGAG
AAGCAGAGGAAGGAGAGGAGAGGGGCACAGGGTCTCTACCATCCCCTGTAGAGTGGGAGCTGAGTGGGGGATCACAGCCTCTGAAAACCAATGTTCTCTC
TTCTCCACCTCCCACAAAGGAGAGCTAGCAGCAGGGAGGGCTTCTGCCATTTCTGAGATCAAAACGGTTTTACTGCAGCTTTGTTTGTTGTCAGCTGAAC
CTGGGTAACTAGGGAAGATAATATTAAGGAAGACAATGTGAAAAGAAAAATGAGCCTGGCAAGAATGTGTTTAAACTTGGTTTTTAAAAAACTGCTGACT
GTTTTCTCTTGAGAGGGTGGAATATCCAATATTCGCTGTGTCAGCATAGAAGTAACTTACTTAGGTGTGGGGGAAGCACCATAACTTTGTTTAGCCCAAA
ACCAAGTCAAGTGAAAAAGGAGGAAGAGAAAAAATATTTTCCTGCCAGGCATGGTGGCCCACGCACTTCGGGAGGTCGAGGCAGGAGGATCACTTGAGTC
CAGAAGTTTGAGATCAGCCTGGGCAATGTGATAAAACCCCATCTCTACAAAAAGCATAAAAATTAGCCAAGTGTGGTAGAGTGTGCCTGAAGTCCCAGAT
ACTTGGGGGGCTGAGGTGGGAGGATCTCTTGAGCCTGGGAGGTCAAGGCTGCAGTGAGCCGAGATTGCACCACTGCACTCCAGCCTGGGTGACAGAGCAA
GTGAGACCCTGTCTCAAAAAAAGAAAAAGAAAAAGAAAAAATATTTTCCCTATTAGAGAAGAGATTGTGGTTTCATTCTGTATTTTGTTTTTGTCTTAAA
AAGTGGAAAAATAGCCTGCCTCTTCTCTACTCTAGGGAAAAACCAGCGTGTGACTACTCCCCCAGGTGGTTATGGAGAGGGTGTCCGGTCCCTGTCCCAG
TGCCGAGAAGGAAGCCTCCCACGACTGCCCGGCAGGGTCCTAGAAATTCCCCACCCTGAAAGCCCTGAGCTTTCTGCTATCAAAGAGGTTTTAAAAAAAT
CCCATTTAAAAAAAATCCCTTACCTCGGTGCCTTCCTCTTTTTATTTAGTTCCTTGAGTTGATTCAGCTCTGCAAGAATTGAAGCAGGACTAAATGTCTA
GTTGTAACACCATGATTAACCACTTCAGCTGACTTTTCTGTCCGAGCTTTGAAAATTCAGTGGTGTTAGTGGTTACCCAGTTAGCTCTCAAGTTATCAGG
GTATTCCAGAGTGGGGATATGATTTAAATCAGCCGTGTAACCATGGACCCAATATTTACCAGACCACAAAACTTTTCTAATACTCTACCCTCTTAGAAAA
ACCACCACCATCACCAGACAGGTGCGAAAGGATGAAAGTGACCATGTTTTGTTTACGGTTTTCCAGGTTTAAGCTGTTACTGTCTTCAGTAAGCCGTGAT
TTTCATTGCTGGGCTTGTCTGTAGATTTTAGACCCTATTGCTGCTTGAGGCAACTCATCTTAGGTTGGCAAAAAGGCAGGATGGCCGGGCGCGGTGGCTC
ACGCCTGTAATCCTAGCACTTTGGGAGGCCAAGGTGGGAGGATTGCTTGAGCTCAGGAGTTTGAGACCAACCTGGG
  • refseq_IL6R.F2 (forward primer)
  • refseq_IL6R.R2 (reverse primer)
  • Single exon skipping

Frame 0

GGPLFSPLRCGAVAGSHPLGRPVRGAVAPSAPA
FVTAPWDGPETLQREFLKCFPALPGPSAALSHVR
VGSRTDTEPGQRERSRARRGAEGLAVCVESRAP
ADGGCPRGLSPPARPPPRPAPATPAARFPLACP
PLRDHGVVAEEEACWPSAARCWLPCWPRRERRWP
QGAALRRRWREAC*PVCQETA*L*PARG*SRKT
MPLFTGCSGSRLQAPTPADGLAWEGGCC*GRCS
STTLETIHATGPAAQLGLCTCWWMFPPRSPSSPA
SGRAPSAMLFVSGVLGAPHP*RQRLCSW*GSFR
TVRPKTSRSRASIPRSPRSSPAS*QSRRETALS
T*CPCASPVVSGASSAKLKPFRVVESCSLIRLPT
SQSLPWPETPAGSVSPGKTPTPGTHLSTDYGLS
SDIGLNGQRHSQHGWSRTSSITVSSTTPGAA*G
TWCSFVPRRSSGKASGASGARRPWARLGQNPGVL
QLRTRCPPPCRHLLLIKTMIIFSSEILQMRQAS
QCKILLQYHCPHSWLLEGAWPSERSSALPLF*G
SRRRGSCGL*RKARQACIRRTLWGSWSRRGLDPP
QCLFLSSPHRCPPAAWGLTIPRATTDQMPGTHG
ALMTSAIQTTSSPDSWLGGTSSLDPVDDKTQTG
SAKDASHCHASLSQGCAAFGFTEEPCGRFYARGK
SACSSCSAG*GFKPPFPNAQLKGARVNLGHCEE
NHIKTLWTLTRTLKSWAGWWGPRCGEAAGSPPL
NTSAQAAPSGRWDGFPAKASSSRHAPGPLHRFIF
QLKLLKPKCLSKFCFSRPGDGFYLNRQGWGKKL
SPPFLPYS*KTAEGEWVNNTVSQGLVVFNRIII
SSSLAFC*M*MMILGIC*IQRQLHWLWVAGPQVR
SRGRRGEGHRVSTIPCRVGAEWGITASENQCSL
FSTSHKGELAAGRASAISEIKTVLLQLCLLSAE
PG*LGKIILRKTM*KEK*AWQECV*TWFLKNC*L
FSLERVEYPIFAVSA*K*LT*VWGKHHNFV*PK
TKSSEKGGREKIFSCQAWWPTHFGRSRQEDHLS
PEV*DQPGQCDKTPSLQKA*KLAKCGRVCLKSQI
LGGLRWEDLLSLGGQGCSEPRLHHCTPAWVTEQ
VRPCLKKRKRKRKNIFPIREEIVVSFCILFLS*
KVEK*PASSLL*GKTSV*LLPQVVMERVSGPCPS
AEKEASHDCPAGS*KFPTLKALSFLLSKRF*KN
PI*KKSLTSVPSSFYLVP*VDSALQELKQD*MS
SCNTMINHFS*LFCPSFENSVVLVVTQLALKLSG
YSRVGI*FKSAV*PWTQYLPDHKTFLILYPLRK
TTTITRQVRKDESDHVLFTVFQV*AVTVFSKP*
FSLLGLSVDFRPYCCLRQLILGWQKGRMAGRGGS
RL*S*HFGRPRWEDCLSSGV*DQPG

Frame 1

AVPCSPRSGAALWQEATPSVGRCAGLLRHPLRL
S*PHPGTAQRRSSASSSNVFLRCQDRPPL*VMC
EWEVALTLSRARGRGAERGAGPRDSQCV*RAGLL
RMGAAPGA*ARLPAHRPAPPLPPLPPGSH*PVR
LCGTMEW*PRRKHAGRRLRAAGCPAGRAGSGAG
PKALPCAGGGERRADQSARRQRDSDLPGGRAGRQ
CHCSLGAQEAGCRLPPQQMGWHGKEAAAEVGAA
PRLWKLFMLPGRPPSWDCALAGGCSPRGAPALL
LPEEPPQQCCL*VGSSEHPIPDDKGCALGEEVSE
QSGRRLPGAVPVFPGVPEVLLPVSSPGGRQLFL
HSVHVRRQ*CREQVQQNSNLSGLWNLAA*SACQ
HHSHCRGQKPPLAQCHLARPPLLELIFLQTTV*A
QISG*TVKDIHNMDGQGPPASLCHPRRLERPEA
RGAASCPGGVRARRVERVEPGGHGHALDRIQES
SS*ERGVHPHAGTYY**RR**YSLQRFCKCDKPP
SARFFFSTTAHIPGCWREPGLRNAPLHCHCSEV
QEDVEAAGSEGRQDKHASAVLFGAAGPGEASTH
PSACSSHLPTGVPQQPGV*QYLEPQPTRCQGPTE
PL*HQQYRLLLPQIAGWVAPAAWTLWMIKHKRA
QQKMLLTAMPAYLRGVRPLASRKSLAEGSTPGE
NQPAPAVQLVEVSNLPFQMPSLKGLE*TWATVKR
TISRLFGHSHGHSKAGQVGGGLGVEKRLAAHPS
TPLHKLHPQAGGMDFQPKPPPAAMLLAHCIVSS
SNSNS*NPSALANSVFLGLGTAFT*TAKAGGRSS
LLPFFPTVEKQLRVSG*IIQYLRAWSFSTEL*L
VPH*HFAKCE**S*AFAEYRGNCIGFGLQDLR*
EAEEGEERGTGSLPSPVEWELSGGSQPLKTNVLS
SPPPTKES*QQGGLLPFLRSKRFYCSFVCCQLN
LGN*GR*Y*GRQCEKKNEPGKNVFKLGF*KTAD
CFLLRGWNIQYSLCQHRSNLLRCGGSTITLFSPK
PSQVKKEEEKKYFPARHGGPRTSGGRGRRIT*V
QKFEISLGNVIKPHLYKKHKN*PSVVECA*SPR
YLGG*GGRIS*AWEVKAAVSRDCTTALQPG*QSK
*DPVSKKEKEKEKIFSLLEKRLWFHSVFCFCLK
KWKNSLPLLYSREKPACDYSPRWLWRGCPVPVP
VPRRKPPTTARQGPRNSPP*KP*AFCYQRGFKKI
PFKKNPLPRCLPLFI*FLELIQLCKN*SRTKCL
VVTP*LTTSADFSVRALKIQWC*WLPS*LSSYQ
GIPEWGYDLNQPCNHGPNIYQTTKLF*YSTLLEK
PPPSPDRCERMKVTMFCLRFSRFKLLLSSVSRD
FHCWACL*ILDPIAA*GNSS*VGKKAGWPGAVA
HACNPSTLGGQGGRIA*AQEFETNL

Frame 2

RSPVLPAQVRRCGRKPPPRSAGARGCCAIRSGF
RNRTLGRPRDAPARVPQMFSCVARTVRRSESCA
SGKSH*H*AGPEGEEPSAARGRGTRSVCREPGS
CGWGLPPGPEPACPPTAPPRPCHPCRPVPISLSA
SAGPWSGSRGGSMLAVGCALLAALLAAPGAALA
PRRCPAQEVARGVLTSLPGDSVTLTCPGVEPED
NATVHWVLRKPAAGSHPSRWAGMGRRLLLRSVQL
HDSGNYSCYRAGRPAGTVHLLVDVPPEEPQLSC
FRKSPLSNVVCEWGPRSTPSLTTKAVLLVRKFQ
NSPAEDFQEPCQYSQESQKFSCQLAVPEGDSSFY
IVSMCVASSVGSKFSKTQTFQGCGILQPDPPAN
ITVTAVARNPRWLSVTWQDPHSWNSSFYRLRFE
LRYRAERSKTFTTWMVKDLQHHCVIHDAWSGLRH
VVQLRAQEEFGQGEWSEWSPEAMGTPWTESRSP
PAENEVSTPMQALTTNKDDDNILFRDSANATSL
PVQDSSSVPLPTFLVAGGSLAFGTLLCIAIVLRF
KKTWKLRALKEGKTSMHPPYSLGQLVPERPRPT
PVLVPLISPPVSPSSLGSDNTSSHNRPDARDPR
SPYDISNTDYFFPR*LAGWHQQPGPCG**NTNGL
SKRCFSLPCQLISGVCGLWLHGRALRKVLRQGK
ISLLQLFSWLRFQTSLSKCPA*RG*SELGPL*R
EPYQDSLDTHTDTQKLGRLVGASVWRSGWQPTPQ
HLCTSCTLRQVGWISSQSLLQPPCSWPTASFHL
PTQTLKTQVP*QILFF*AWGRLLLKPPRLGEEA
LSSLSSLQLKNS*G*VGE*YSISGPGRFQQNYN*
FLISILLNVNDDPRHLLNTEATALALGCRTSGE
KQRKERRGAQGLYHPL*SGS*VGDHSL*KPMFS
LLHLPQRRASSREGFCHF*DQNGFTAALFVVS*T
WVTREDNIKEDNVKRKMSLARMCLNLVFKKLLT
VFS*EGGISNIRCVSIEVTYLGVGEAP*LCLAQ
NQVK*KRRKRKNIFLPGMVAHALREVEAGGSLES
RSLRSAWAM**NPISTKSIKISQVW*SVPEVPD
TWGAEVGGSLEPGRSRLQ*AEIAPLHSSLGDRA
SETLSQKKKKKKKKYFPY*RRDCGFILYFVFVLK
SGKIACLFSTLGKNQRVTTPPGGYGEGVRSLSQ
CREGSLPRLPGRVLEIPHPESPELSAIKEVLKK
SHLKKIPYLGAFLFLFSSLS*FSSARIEAGLNV*
L*HHD*PLQLTFLSEL*KFSGVSGYPVSSQVIR
VFQSGDMI*ISRVTMDPIFTRPQNFSNTLPS*K
NHHHHQTGAKG*K*PCFVYGFPGLSCYCLQ*AVI
FIAGLVCRF*TLLLLEATHLRLAKRQDGRARWL
TPVILALWEAKVGGLLELRSLRPTW

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