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Sequence NM_080426
CCGCCGCCGCCGCAGCCCGGCCGCGCCCCGCCGCCGCCGCCGCCGCCATGGGCTGCCTCGGGAACAGTAAGACCGAGGACCAGCGCAACGAGGAGAAGGCGCAGCGTGAGGCCAACAAAAAGATCGAGAAGCAGCTGCAGAAGGACAAGCAGGTCTACCGGGCCACGCACCGCCTGCTGCTGCTGGGTGCTGGAGAATCT
GGTAAAAGCACCATTGTGAAGCAGATGAGGATCCTGCATGTTAATGGGTTTAATGGAGACAGTGAGAAGGCAACCAAAGTGCAGGACATCAAAAACAACC
TGAAAGAGGCGATTGAAACCATTGTGGCCGCCATGAGCAACCTGGTGCCCCCCGTGGAGCTGGCCAACCCCGAGAACCAGTTCAGAGTGGACTACATCCT
GAGTGTGATGAACGTGCCTGACTTTGACTTCCCTCCCGAATTCTATGAGCATGCCAAGGCTCTGTGGGAGGATGAAGGAGTGCGTGCCTGCTACGAACGC
TCCAACGAGTACCAGCTGATTGACTGTGCCCAGTACTTCCTGGACAAGATCGACGTGATCAAGCAGGCTGACTATGTGCCGAGCGATCAGGACCTGCTTC
GCTGCCGTGTCCTGACTTCTGGAATCTTTGAGACCAAGTTCCAGGTGGACAAAGTCAACTTCCACATGTTTGACGTGGGTGGCCAGCGCGATGAACGCCG
CAAGTGGATCCAGTGCTTCAACGATGTGACTGCCATCATCTTCGTGGTGGCCAGCAGCAGCTACAACATGGTCATCCGGGAGGACAACCAGACCAACCGC
CTGCAGGAGGCTCTGAACCTCTTCAAGAGCATCTGGAACAACAGATGGCTGCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG
AGAAAGTCCTTGCTGGGAAATCGAAGATTGAGGACTACTTTCCAGAATTTGCTCGCTACACTACTCCTGAGGATGCTACTCCCGAGCCCGGAGAGGACCC
ACGCGTGACCCGGGCCAAGTACTTCATTCGAGATGAGTTTCTGAGGATCAGCACTGCCAGTGGAGATGGGCGTCACTACTGCTACCCTCATTTCACCTGC
GCTGTGGACACTGAGAACATCCGCCGTGTGTTCAACGACTGCCGTGACATCATTCAGCGCATGCACCTTCGTCAGTACGAGCTGCTCTAAGAAGGGAACC
CCCAAATTTAATTAAAGCCTTAAGCACAATTAATTAAAAGTGAAACGTAATTGTACAAGCAGTTAATCACCCACCATAGGGCATGATTAACAAAGCAACC
TTTCCCTTCCCCCGAGTGATTTTGCGAAACCCCCTTTTCCCTTCAGCTTGCTTAGATGTTCCAAATTTAGAAAGCTTAAGGCGGCCTACAGAAAAAGGAA
AAAAGGCCACAAAAGTTCCCTCTCACTTTCAGTAAAAATAAATAAAACAGCAGCAGCAAACAAATAAAATGAAATAAAAGAAACAAATGAAATAAATATT
GTGTTGTGCAGCATTAAAAAAAATCAAAATAAAAATTAAATGTGAGCAAAGAA
- refseq_GNAS.F1 (forward primer)
- refseq_GNAS.R1 (reverse primer)
- Mutually exclusive exon skipping
Frame 0
PPPPQPGRAPPPPPPPWAASGTVRPRTSATRRRRSVRPTKRSRSSCRRTSRSTGPRTACCCWVLENL
VKAPL*SR*GSCMLMGLMETVRRQPKCRTSKTT
*KRRLKPLWPP*ATWCPPWSWPTPRTSSEWTTS
*V**TCLTLTSLPNSMSMPRLCGRMKECVPATNA
PTSTS*LTVPSTSWTRST*SSRLTMCRAIRTCF
AAVS*LLESLRPSSRWTKSTSTCLTWVASAMNA
ASGSSASTM*LPSSSWWPAAATTWSSGRTTRPTA
CRRL*TSSRASGTTDGCAPSL*SCSSTSKICSL
RKSLLGNRRLRTTFQNLLATLLLRMLLPSPERT
HA*PGPSTSFEMSF*GSALPVEMGVTTATLISPA
LWTLRTSAVCSTTAVTSFSACTFVSTSCSKKGT
PKFN*SLKHN*LKVKRNCTSS*SPTIGHD*QSN
LSLPPSDFAKPPFPFSLLRCSKFRKLKAAYRKRK
KGHKSSLSLSVKINKTAAANK*NEIKETNEINI
VLCSIKKNQNKN*M*AK
Frame 1
RRRRSPAAPRRRRRRHGLPREQ*DRGPAQRGEGAA*GQQKDREAAAEGQAGLPGHAPPAAAGCWRI
W*KHHCEADEDPAC*WV*WRQ*EGNQSAGHQKQP
ERGD*NHCGRHEQPGAPRGAGQPREPVQSGLHP
ECDERA*L*LPSRIL*ACQGSVGG*RSACLLRT
LQRVPAD*LCPVLPGQDRRDQAG*LCAERSGPAS
LPCPDFWNL*DQVPGGQSQLPHV*RGWPAR*TP
QVDPVLQRCDCHHLRGGQQQLQHGHPGGQPDQP
PAGGSEPLQEHLEQQMAAHHLCDPVPQQARSAR*
ESPCWEIED*GLLSRICSLHYS*GCYSRARRGP
TRDPGQVLHSR*VSEDQHCQWRWASLLLPSFHL
RCGH*EHPPCVQRLP*HHSAHAPSSVRAALRREP
PNLIKALSTIN*K*NVIVQAVNHPP*GMINKAT
FPFPRVILRNPLFPSACLDVPNLESLRRPTEKG
KKATKVPSHFQ*K*IKQQQQTNKMK*KKQMK*IL
CCAALKKIKIKIKCEQR
Frame 2
AAAAARPRPAAAAAAMGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGES
GKSTIVKQMRILHVNGFNGDSEKATKVQDIKNN
LKEAIETIVAAMSNLVPPVELANPENQFRVDYIL
SVMNVPDFDFPPEFYEHAKALWEDEGVRACYER
SNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLL
RCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERR
KWIQCFNDVTAIIFVVASSSYNMVIREDNQTNR
LQEALNLFKSIWNNRWLRTISVILFLNKQDLLA
EKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDP
RVTRAKYFIRDEFLRISTASGDGRHYCYPHFTC
AVDTENIRRVFNDCRDIIQRMHLRQYELL*EGN
PQI*LKP*AQLIKSET*LYKQLITHHRA*LTKQP
FPSPE*FCETPFSLQLA*MFQI*KA*GGLQKKE
KRPQKFPLTFSKNK*NSSSKQIK*NKRNK*NKY
CVVQH*KKSK*KLNVSKE
Sequence NM_000516
CCGCCGCCGCCGCAGCCCGGCCGCGCCCCGCCGCCGCCGCCGCCGCCATGGGCTGCCTCGGGAACAGTAAGACCGAGGACCAGCGCAACGAGGAGAAGGCGCAGCGTGAGGCCAACAAAAAGATCGAGAAGCAGCTGCAGAAGGACAAGCAGGTCTACCGGGCCACGCACCGCCTGCTGCTGCTGGGTGCTGGAGAATCT
GGTAAAAGCACCATTGTGAAGCAGATGAGGATCCTGCATGTTAATGGGTTTAATGGAGAGGGCGGCGAAGAGGACCCGCAGGCTGCAAGGAGCAACAGCG
ATGGTGAGAAGGCAACCAAAGTGCAGGACATCAAAAACAACCTGAAAGAGGCGATTGAAACCATTGTGGCCGCCATGAGCAACCTGGTGCCCCCCGTGGA
GCTGGCCAACCCCGAGAACCAGTTCAGAGTGGACTACATCCTGAGTGTGATGAACGTGCCTGACTTTGACTTCCCTCCCGAATTCTATGAGCATGCCAAG
GCTCTGTGGGAGGATGAAGGAGTGCGTGCCTGCTACGAACGCTCCAACGAGTACCAGCTGATTGACTGTGCCCAGTACTTCCTGGACAAGATCGACGTGA
TCAAGCAGGCTGACTATGTGCCGAGCGATCAGGACCTGCTTCGCTGCCGTGTCCTGACTTCTGGAATCTTTGAGACCAAGTTCCAGGTGGACAAAGTCAA
CTTCCACATGTTTGACGTGGGTGGCCAGCGCGATGAACGCCGCAAGTGGATCCAGTGCTTCAACGATGTGACTGCCATCATCTTCGTGGTGGCCAGCAGC
AGCTACAACATGGTCATCCGGGAGGACAACCAGACCAACCGCCTGCAGGAGGCTCTGAACCTCTTCAAGAGCATCTGGAACAACAGATGGCTGCGCACCA
TCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTGAGAAAGTCCTTGCTGGGAAATCGAAGATTGAGGACTACTTTCCAGAATTTGCTCGCTA
CACTACTCCTGAGGATGCTACTCCCGAGCCCGGAGAGGACCCACGCGTGACCCGGGCCAAGTACTTCATTCGAGATGAGTTTCTGAGGATCAGCACTGCC
AGTGGAGATGGGCGTCACTACTGCTACCCTCATTTCACCTGCGCTGTGGACACTGAGAACATCCGCCGTGTGTTCAACGACTGCCGTGACATCATTCAGC
GCATGCACCTTCGTCAGTACGAGCTGCTCTAAGAAGGGAACCCCCAAATTTAATTAAAGCCTTAAGCACAATTAATTAAAAGTGAAACGTAATTGTACAA
GCAGTTAATCACCCACCATAGGGCATGATTAACAAAGCAACCTTTCCCTTCCCCCGAGTGATTTTGCGAAACCCCCTTTTCCCTTCAGCTTGCTTAGATG
TTCCAAATTTAGAAAGCTTAAGGCGGCCTACAGAAAAAGGAAAAAAGGCCACAAAAGTTCCCTCTCACTTTCAGTAAAAATAAATAAAACAGCAGCAGCA
AACAAATAAAATGAAATAAAAGAAACAAATGAAATAAATATTGTGTTGTGCAGCATTAAAAAAAATCAAAATAAAAATTAAATGTGAGCAAAGAA
- refseq_GNAS.F1 (forward primer)
- refseq_GNAS.R1 (reverse primer)
- Mutually exclusive exon skipping
Frame 0
PPPPQPGRAPPPPPPPWAASGTVRPRTSATRRRRSVRPTKRSRSSCRRTSRSTGPRTACCCWVLENL
VKAPL*SR*GSCMLMGLMERAAKRTRRLQGATA
MVRRQPKCRTSKTT*KRRLKPLWPP*ATWCPPW
SWPTPRTSSEWTTS*V**TCLTLTSLPNSMSMPR
LCGRMKECVPATNAPTSTS*LTVPSTSWTRST*
SSRLTMCRAIRTCFAAVS*LLESLRPSSRWTKS
TSTCLTWVASAMNAASGSSASTM*LPSSSWWPAA
ATTWSSGRTTRPTACRRL*TSSRASGTTDGCAP
SL*SCSSTSKICSLRKSLLGNRRLRTTFQNLLA
TLLLRMLLPSPERTHA*PGPSTSFEMSF*GSALP
VEMGVTTATLISPALWTLRTSAVCSTTAVTSFS
ACTFVSTSCSKKGTPKFN*SLKHN*LKVKRNCT
SS*SPTIGHD*QSNLSLPPSDFAKPPFPFSLLRC
SKFRKLKAAYRKRKKGHKSSLSLSVKINKTAAA
NK*NEIKETNEINIVLCSIKKNQNKN*M*AK
Frame 1
RRRRSPAAPRRRRRRHGLPREQ*DRGPAQRGEGAA*GQQKDREAAAEGQAGLPGHAPPAAAGCWRI
W*KHHCEADEDPAC*WV*WRGRRRGPAGCKEQQR
W*EGNQSAGHQKQPERGD*NHCGRHEQPGAPRG
AGQPREPVQSGLHPECDERA*L*LPSRIL*ACQ
GSVGG*RSACLLRTLQRVPAD*LCPVLPGQDRRD
QAG*LCAERSGPASLPCPDFWNL*DQVPGGQSQ
LPHV*RGWPAR*TPQVDPVLQRCDCHHLRGGQQ
QLQHGHPGGQPDQPPAGGSEPLQEHLEQQMAAHH
LCDPVPQQARSAR*ESPCWEIED*GLLSRICSL
HYS*GCYSRARRGPTRDPGQVLHSR*VSEDQHC
QWRWASLLLPSFHLRCGH*EHPPCVQRLP*HHSA
HAPSSVRAALRREPPNLIKALSTIN*K*NVIVQ
AVNHPP*GMINKATFPFPRVILRNPLFPSACLD
VPNLESLRRPTEKGKKATKVPSHFQ*K*IKQQQQ
TNKMK*KKQMK*ILCCAALKKIKIKIKCEQR
Frame 2
AAAAARPRPAAAAAAMGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGES
GKSTIVKQMRILHVNGFNGEGGEEDPQAARSNS
DGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVE
LANPENQFRVDYILSVMNVPDFDFPPEFYEHAK
ALWEDEGVRACYERSNEYQLIDCAQYFLDKIDV
IKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVN
FHMFDVGGQRDERRKWIQCFNDVTAIIFVVASS
SYNMVIREDNQTNRLQEALNLFKSIWNNRWLRT
ISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARY
TTPEDATPEPGEDPRVTRAKYFIRDEFLRISTA
SGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQ
RMHLRQYELL*EGNPQI*LKP*AQLIKSET*LYK
QLITHHRA*LTKQPFPSPE*FCETPFSLQLA*M
FQI*KA*GGLQKKEKRPQKFPLTFSKNK*NSSS
KQIK*NKRNK*NKYCVVQH*KKSK*KLNVSKE
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