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Sequence NM_001025249
GTCATGTTCCCAGGACGGGCGCGTCTTCAGGGTGGAAGCCTGGCGCACGTCCGGAGAGACACCCGCCATTTCACCCAGTAAGCGGGCCCGGCCTGCGGAGGTGGGCGGCATGCAGCTCCGCTTTGCCCGGCTCTCCGAGCACGCCACGGCCCCCACCCGGGGCTCCGCGCGCGCCGCGGGCTACGACCTGTACAGTGCCT
ATGATTACACAATACCACCTATGGAGAAAGCTGTTGTGAAAACGGACATTCAGATAGCGCTCCCTTCTGGGTGTTATGGAAGAGTGGCTCCACGGTCAGG
CTTGGCTGCAAAACACTTTATTGATGTAGGAGCTGGTGTCATAGATGAAGATTATAGAGGAAATGTTGGTGTTGTACTGTTTAATTTTGGCAAAGAAAAG
TTTGAAGTCAAAAAAGGTGATCGAATTGCACAGCTCATTTGCGAACGGATTTTTTATCCAGAAATAGAAGAAGTTCAAGCCTTGGATGACACCGAAAGGG
GTTCAGGAGGTTTTGGTTCCACTGGAAAGAATTAAAATTTATGCCAAGAACAGAAAACAAGAAGTCATACCTTTTTCTTAAAAAAAAAAAAAAAGTTTTT
GCTTCAAGTGTTTTGGTGTTTTGCACTTCTGTAAACTTACTAGCTTTACCTTCTAAAAGTACTGCATTTTTTACTTTTTTTTATGATCAAGGAAAAGATC
ATTAAAAAAAAACACAAAGAAGTTTTTCTTTGTGTTTGGATCAAAAAGAAACTTTGTTTTTCCGCAATTGAAGGTTGTATGTAAATCTGCTTTGTGGTGA
CCTGATGTAAACAGTGTCTTCTTAAAATCAAATGTAAATCAATTACAGATTAAAAAAAAAAGCCTGTATTTAACTCATATGATCTCCCTTCAGCAACTTA
TTTTGCTTTAATTGCTTTAAATCTTAAGCAATATTTTTTATTCAGTAAACAAATTCTTTCACAAGGTACAAAATCTTGCATAAGCTGAACTAAAATAAAA
ATGAAAAGGAGAGATTAAAGGTATTCCTTGTTCTTCCCTTCTCTTCACTAGTCTAAAAACTTCTTTTTAATCTTAAGATTCTTTGTGATGAGGGTGAGAA
AAAGAATCCTCAGTTTATTTTTCCACTATTAATCTTTCTTTTGATAAATCCTCTATTGACTGGGTAGAGGTATGTTTGTGAAAGACATGTAACTTGGGGA
TTTGTTACTTTAGGTTTGTTCCCTTGAATTTCATCTCATCAGGCAAATTGTACTAGTTGTAGTTACGAGTTTTCCCTCAGTGAAGTAGCAATAGGCTGTA
ATCAAGAAAATATGCCATTTATAGAGATAAGATAAATGAAATAATACTTCAGCCACCAGGTTTTTCTGTCTCACATACATAAGCAGCATTTCATTGCAGA
TATGGGACTGATTCTGTGGCTTACCTTGATTAACATCTTTTGGAAGTTTTGCTAGTGTGCTTTCCTTTCTTTACTATGTTTCTCAGATTCCTTTGTATCA
GGGTTTTGGGTGTCACTTAGGTTTTGTCCATCAGATTCTGTGAGACACCAGGCATCGTTTTGAGGATGTGGGTTATACACATGGAGTGCTTCTGGAACTA
TCAGCCCACTTGACCACCCAGTTTGTGGAAGCACAGGCAAGAGTGTTCTTTTCTGGTGATTCTCCAGGCCATTTAATACCCTGCAATGTAATTGTCCCTC
TGTGGCTCACATTTCATTAGTGAGCCATGAAATCAACTCAGTGGGACATAGCCAGCATTTTTGCATACCAGGTTGGGCTATAAAATATTTCTGTTGTCAA
TAAATTTTAAATGTTTTCCTGCTAAACTAA
- refseq_DUT.F1 (forward primer)
- refseq_DUT.R1 (reverse primer)
- Alternative donor
Frame 0
VMFPGRARLQGGSLAHVRRDTRHFTQ*AGPACGGGRHAAPLCPALRARHGPHPGLRARRGLRPVQCL
*LHNTTYGESCCENGHSDSAPFWVLWKSGSTVR
LGCKTLY*CRSWCHR*RL*RKCWCCTV*FWQRK
V*SQKR*SNCTAHLRTDFLSRNRRSSSLG*HRKG
FRRFWFHWKELKFMPRTENKKSYLFLKKKKKVF
ASSVLVFCTSVNLLALPSKSTAFFTFFYDQGKD
H*KKTQRSFSLCLDQKETLFFRN*RLYVNLLCGD
LM*TVSS*NQM*INYRLKKKACI*LI*SPFSNL
FCFNCFKS*AIFFIQ*TNSFTRYKILHKLN*NK
NEKERLKVFLVLPFSSLV*KLLFNLKILCDEGEK
KNPQFIFPLLIFLLINPLLTG*RYVCERHVTWG
FVTLGLFP*ISSHQANCTSCSYEFSLSEVAIGC
NQENMPFIEIR*MK*YFSHQVFLSHIHKQHFIAD
MGLILWLTLINIFWKFC*CAFLSLLCFSDSFVS
GFWVSLRFCPSDSVRHQASF*GCGLYTWSASGT
ISPLDHPVCGSTGKSVLFW*FSRPFNTLQCNCPS
VAHISLVSHEINSVGHSQHFCIPGWAIKYFCCQ
*ILNVFLLN*
Frame 1
SCSQDGRVFRVEAWRTSGETPAISPSKRARPAEVGGMQLRFARLSEHATAPTRGSARAAGYDLYSA
YDYTIPPMEKAVVKTDIQIALPSGCYGRVAPRSG
LAAKHFIDVGAGVIDEDYRGNVGVVLFNFGKEK
FEVKKGDRIAQLICERIFYPEIEEVQALDDTER
GSGGFGSTGKN*NLCQEQKTRSHTFFLKKKKKFL
LQVFWCFALL*TY*LYLLKVLHFLLFFMIKEKI
IKKKHKEVFLCVWIKKKLCFSAIEGCM*ICFVV
T*CKQCLLKIKCKSITD*KKKPVFNSYDLPSATY
FALIALNLKQYFLFSKQILSQGTKSCIS*TKIK
MKRRD*RYSLFFPSLH*SKNFFLILRFFVMRVR
KRILSLFFHY*SFF**ILY*LGRGMFVKDM*LGD
LLL*VCSLEFHLIRQIVLVVVTSFPSVK*Q*AV
IKKICHL*R*DK*NNTSATRFFCLTYISSISLQ
IWD*FCGLP*LTSFGSFASVLSFLYYVSQIPLYQ
GFGCHLGFVHQIL*DTRHRFEDVGYTHGVLLEL
SAHLTTQFVEAQARVFFSGDSPGHLIPCNVIVP
LWLTFH**AMKSTQWDIASIFAYQVGL*NISVVN
KF*MFSC*T
Frame 2
HVPRTGASSGWKPGARPERHPPFHPVSGPGLRRWAACSSALPGSPSTPRPPPGAPRAPRATTCTVP
MITQYHLWRKLL*KRTFR*RSLLGVMEEWLHGQ
AWLQNTLLM*ELVS*MKIIEEMLVLYCLILAKKS
LKSKKVIELHSSFANGFFIQK*KKFKPWMTPKG
VQEVLVPLERIKIYAKNRKQEVIPFS*KKKKSF
CFKCFGVLHFCKLTSFTF*KYCIFYFFL*SRKRS
LKKNTKKFFFVFGSKRNFVFPQLKVVCKSALW*
PDVNSVFLKSNVNQLQIKKKSLYLTHMISLQQL
ILL*LL*ILSNIFYSVNKFFHKVQNLA*AELK*K
*KGEIKGIPCSSLLFTSLKTSF*S*DSL**G*E
KESSVYFSTINLSFDKSSIDWVEVCL*KTCNLG
ICYFRFVPLNFISSGKLY*L*LRVFPQ*SSNRL*
SRKYAIYRDKINEIILQPPGFSVSHT*AAFHCR
YGTDSVAYLD*HLLEVLLVCFPFFTMFLRFLCI
RVLGVT*VLSIRFCETPGIVLRMWVIHMECFWNY
QPT*PPSLWKHRQECSFLVILQAI*YPAM*LSL
CGSHFISEP*NQLSGT*PAFLHTRLGYKIFLLS
INFKCFPAKL
Sequence NM_001025248
GTCATGTTCCCAGGACGGGCGCGTCTTCAGGGTGGAAGCCTGGCGCACGTCCGGAGGTGCCGAGGACCCAACCAGCCCAAACTCTGGGGGAAATGACTCCCCTCTGCCCTCGCCCCGCGCTCTGCTACCATTTCCTTACGTCTCTGCTTCGCTCAGCGATGCAAAACGCGCGAGGCGCACGGCAGAGGGCCGAAGCCGCG
GTACTCTCCGGGCCAGGCCCGCCCCTCGGCCGCGCCGCGCAGCACGGGATTCCCCGGCCGCTGTCCAGCGCTGGCCGCCTGAGCCAAGGCTGCCGCGGAG
CCAGTACAGTCGGGGCCGCTGGCTGGAAGGGCGAGCTTCCTAAGGCGGGGGGAAGCCCGGCGCCGGGGCCGGAGACACCCGCCATTTCACCCAGTAAGCG
GGCCCGGCCTGCGGAGGTGGGCGGCATGCAGCTCCGCTTTGCCCGGCTCTCCGAGCACGCCACGGCCCCCACCCGGGGCTCCGCGCGCGCCGCGGGCTAC
GACCTGTACAGTGCCTATGATTACACAATACCACCTATGGAGAAAGCTGTTGTGAAAACGGACATTCAGATAGCGCTCCCTTCTGGGTGTTATGGAAGAG
TGGCTCCACGGTCAGGCTTGGCTGCAAAACACTTTATTGATGTAGGAGCTGGTGTCATAGATGAAGATTATAGAGGAAATGTTGGTGTTGTACTGTTTAA
TTTTGGCAAAGAAAAGTTTGAAGTCAAAAAAGGTGATCGAATTGCACAGCTCATTTGCGAACGGATTTTTTATCCAGAAATAGAAGAAGTTCAAGCCTTG
GATGACACCGAAAGGGGTTCAGGAGGTTTTGGTTCCACTGGAAAGAATTAAAATTTATGCCAAGAACAGAAAACAAGAAGTCATACCTTTTTCTTAAAAA
AAAAAAAAAAGTTTTTGCTTCAAGTGTTTTGGTGTTTTGCACTTCTGTAAACTTACTAGCTTTACCTTCTAAAAGTACTGCATTTTTTACTTTTTTTTAT
GATCAAGGAAAAGATCATTAAAAAAAAACACAAAGAAGTTTTTCTTTGTGTTTGGATCAAAAAGAAACTTTGTTTTTCCGCAATTGAAGGTTGTATGTAA
ATCTGCTTTGTGGTGACCTGATGTAAACAGTGTCTTCTTAAAATCAAATGTAAATCAATTACAGATTAAAAAAAAAAGCCTGTATTTAACTCATATGATC
TCCCTTCAGCAACTTATTTTGCTTTAATTGCTTTAAATCTTAAGCAATATTTTTTATTCAGTAAACAAATTCTTTCACAAGGTACAAAATCTTGCATAAG
CTGAACTAAAATAAAAATGAAAAGGAGAGATTAAAGGTATTCCTTGTTCTTCCCTTCTCTTCACTAGTCTAAAAACTTCTTTTTAATCTTAAGATTCTTT
GTGATGAGGGTGAGAAAAAGAATCCTCAGTTTATTTTTCCACTATTAATCTTTCTTTTGATAAATCCTCTATTGACTGGGTAGAGGTATGTTTGTGAAAG
ACATGTAACTTGGGGATTTGTTACTTTAGGTTTGTTCCCTTGAATTTCATCTCATCAGGCAAATTGTACTAGTTGTAGTTACGAGTTTTCCCTCAGTGAA
GTAGCAATAGGCTGTAATCAAGAAAATATGCCATTTATAGAGATAAGATAAATGAAATAATACTTCAGCCACCAGGTTTTTCTGTCTCACATACATAAGC
AGCATTTCATTGCAGATATGGGACTGATTCTGTGGCTTACCTTGATTAACATCTTTTGGAAGTTTTGCTAGTGTGCTTTCCTTTCTTTACTATGTTTCTC
AGATTCCTTTGTATCAGGGTTTTGGGTGTCACTTAGGTTTTGTCCATCAGATTCTGTGAGACACCAGGCATCGTTTTGAGGATGTGGGTTATACACATGG
AGTGCTTCTGGAACTATCAGCCCACTTGACCACCCAGTTTGTGGAAGCACAGGCAAGAGTGTTCTTTTCTGGTGATTCTCCAGGCCATTTAATACCCTGC
AATGTAATTGTCCCTCTGTGGCTCACATTTCATTAGTGAGCCATGAAATCAACTCAGTGGGACATAGCCAGCATTTTTGCATACCAGGTTGGGCTATAAA
ATATTTCTGTTGTCAATAAATTTTAAATGTTTTCCTGCTAAACTAA
- refseq_DUT.F1 (forward primer)
- refseq_DUT.R1 (reverse primer)
- Alternative donor
Frame 0
VMFPGRARLQGGSLAHVRRCRGPNQPKLWGK*LPSALAPRSATISLRLCFAQRCKTREAHGRGPKPR
YSPGQARPSAAPRSTGFPGRCPALAA*AKAAAE
PVQSGPLAGRASFLRRGEARRRGRRHPPFHPVS
GPGLRRWAACSSALPGSPSTPRPPPGAPRAPRAT
TCTVPMITQYHLWRKLL*KRTFR*RSLLGVMEE
WLHGQAWLQNTLLM*ELVS*MKIIEEMLVLYCL
ILAKKSLKSKKVIELHSSFANGFFIQK*KKFKPW
MTPKGVQEVLVPLERIKIYAKNRKQEVIPFS*K
KKKSFCFKCFGVLHFCKLTSFTF*KYCIFYFFL
*SRKRSLKKNTKKFFFVFGSKRNFVFPQLKVVCK
SALW*PDVNSVFLKSNVNQLQIKKKSLYLTHMI
SLQQLILL*LL*ILSNIFYSVNKFFHKVQNLA*
AELK*K*KGEIKGIPCSSLLFTSLKTSF*S*DSL
**G*EKESSVYFSTINLSFDKSSIDWVEVCL*K
TCNLGICYFRFVPLNFISSGKLY*L*LRVFPQ*
SSNRL*SRKYAIYRDKINEIILQPPGFSVSHT*A
AFHCRYGTDSVAYLD*HLLEVLLVCFPFFTMFL
RFLCIRVLGVT*VLSIRFCETPGIVLRMWVIHM
ECFWNYQPT*PPSLWKHRQECSFLVILQAI*YPA
M*LSLCGSHFISEP*NQLSGT*PAFLHTRLGYK
IFLLSINFKCFPAKL
Frame 1
SCSQDGRVFRVEAWRTSGGAEDPTSPNSGGNDSPLPSPRALLPFPYVSASLSDAKRARRTAEGRSR
GTLRARPAPRPRRAARDSPAAVQRWPPEPRLPRS
QYSRGRWLEGRAS*GGGKPGAGAGDTRHFTQ*A
GPACGGGRHAAPLCPALRARHGPHPGLRARRGL
RPVQCL*LHNTTYGESCCENGHSDSAPFWVLWKS
GSTVRLGCKTLY*CRSWCHR*RL*RKCWCCTV*
FWQRKV*SQKR*SNCTAHLRTDFLSRNRRSSSL
G*HRKGFRRFWFHWKELKFMPRTENKKSYLFLKK
KKKVFASSVLVFCTSVNLLALPSKSTAFFTFFY
DQGKDH*KKTQRSFSLCLDQKETLFFRN*RLYV
NLLCGDLM*TVSS*NQM*INYRLKKKACI*LI*S
PFSNLFCFNCFKS*AIFFIQ*TNSFTRYKILHK
LN*NKNEKERLKVFLVLPFSSLV*KLLFNLKIL
CDEGEKKNPQFIFPLLIFLLINPLLTG*RYVCER
HVTWGFVTLGLFP*ISSHQANCTSCSYEFSLSE
VAIGCNQENMPFIEIR*MK*YFSHQVFLSHIHK
QHFIADMGLILWLTLINIFWKFC*CAFLSLLCFS
DSFVSGFWVSLRFCPSDSVRHQASF*GCGLYTW
SASGTISPLDHPVCGSTGKSVLFW*FSRPFNTL
QCNCPSVAHISLVSHEINSVGHSQHFCIPGWAIK
YFCCQ*ILNVFLLN*
Frame 2
HVPRTGASSGWKPGARPEVPRTQPAQTLGEMTPLCPRPALCYHFLTSLLRSAMQNARGARQRAEAA
VLSGPGPPLGRAAQHGIPRPLSSAGRLSQGCRG
ASTVGAAGWKGELPKAGGSPAPGPETPAISPSKR
ARPAEVGGMQLRFARLSEHATAPTRGSARAAGY
DLYSAYDYTIPPMEKAVVKTDIQIALPSGCYGR
VAPRSGLAAKHFIDVGAGVIDEDYRGNVGVVLFN
FGKEKFEVKKGDRIAQLICERIFYPEIEEVQAL
DDTERGSGGFGSTGKN*NLCQEQKTRSHTFFLK
KKKKFLLQVFWCFALL*TY*LYLLKVLHFLLFFM
IKEKIIKKKHKEVFLCVWIKKKLCFSAIEGCM*
ICFVVT*CKQCLLKIKCKSITD*KKKPVFNSYD
LPSATYFALIALNLKQYFLFSKQILSQGTKSCIS
*TKIKMKRRD*RYSLFFPSLH*SKNFFLILRFF
VMRVRKRILSLFFHY*SFF**ILY*LGRGMFVK
DM*LGDLLL*VCSLEFHLIRQIVLVVVTSFPSVK
*Q*AVIKKICHL*R*DK*NNTSATRFFCLTYIS
SISLQIWD*FCGLP*LTSFGSFASVLSFLYYVS
QIPLYQGFGCHLGFVHQIL*DTRHRFEDVGYTHG
VLLELSAHLTTQFVEAQARVFFSGDSPGHLIPC
NVIVPLWLTFH**AMKSTQWDIASIFAYQVGL*
NISVVNKF*MFSC*T
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