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Sequences

Sequence NM_001009960

TGGCAGCTTGTTTTGCCTTCACGGGAGTAGAAGGAGGCGGCGTCCGGCCGCGGCCGACGGTAGTTCGCTTCCCCGAGAGTGCGCGGAGGCCCGGGTGCGA
GGAGGGCCTGTTTCTCTTCAGCCCTGGTTCATTCACCTCGCGGACCGAGGGCCCCGCCGTCAGGAGCCGGCGACCGTGCCCTGGTGCGAGCTGTGATGGT
CATTGTCCTCCAGAGCAGTGATGGAGGAAATACCAGCCCAGGAAGCAGCAGGGTCACCAAGGGTCCAGTTTCAGTCTTTGGAGACCCAGTCTGAGTGTCT
GTCCCCAGAGCCTCAGTTTGTGCAGGACACCGACATGGAACAGGGACTCACTGGGGATGGAGAGACCAGAGAAGAGAACAAGCTGTTGATTCCTAAGCAG
AAAATTTCGGAAGAAGTGCATTCATACAAAGTGAGAGTAGGAAGACTCAAACACGATATTACCCAAGTTCCTGAGACTAGAGAAGTGTATAAGTCTGAGG
ACAGATTAGAAAGACTTCAGGAAATTCTAAGGAAATTTCTGTACCTGGAGAGAGAGTTTAGGCAAATAACAATCAGCAAGGAAACCTTCACCAGTGAGAA
GAACAATGAATGTCATGAACCCGAAAAAAGCTTCAGTCTGGACTCTACTATTGATGCAGATCAGAGAGTTCTTAGAATACAGAATACCGATGACAATGAT
AAGTATGACATGAGCTTCAACCAGAATTCAGCCTCTGGTAAACATGAACACTTAAATCTAACAGAGGATTTTCAGAGTAGTGAATGTAAGGAAAGCTTAA
TGGATCTCTCCCACCTTAATAAATGGGAGAGCATCCCTAACACTGAGAAATCCTATAAATGTGATGTATGTGGGAAAATTTTCCATCAGAGCTCAGCCCT
TACTAGACATCAGAGAATCCATACTAGAGAGAAGCCCTACAAATGTAAAGAATGTGAAAAGTCTTTCAGTCAGAGCTCAAGTCTTAGTCGACATAAAAGA
ATACACACTAGAGAAAAACCTTACAAATGTGAAGCATCTGATAAATCCTGTGAAGCGTCTGATAAATCCTGTAGTCCAAGCTCAGGCATAATTCAGCATA
AGAAAATTCACACCAGAGCCAAATCTTACAAATGTAGCAGTTGTGAAAGAGTCTTCAGTCGTAGTGTCCACCTTACTCAACATCAGAAAATTCACAAAGA
GATGCCCTGTAAGTGTACTGTATGTGGCAGTGACTTCTGCCATACTTCATACCTACTTGAACATCAGAGGGTCCATCATGAAGAGAAAGCCTATGAGTAT
GATGAATATGGGTTGGCCTATATTAAACAACAAGGAATTCATTTCAGAGAAAAGCCCTATACGTGTAGTGAATGTGGAAAAGACTTCAGATTGAATTCAC
ATCTTATTCAGCATCAAAGAATTCACACAGGAGAGAAAGCACATGAATGTAATGAATGTGGAAAAGCTTTCAGTCAAACCTCATGCCTTATTCAGCATCA
CAAAATGCATAGGAAAGAGAAATCGTATGAATGTAATGAGTATGAGGGCAGTTTCAGTCATAGCTCAGATCTTATCCTGCAACAAGAAGTCCTCACCAGA
CAGAAAGCCTTTGATTGTGATGTATGGGAAAAGAACTCCAGTCAGAGAGCACATCTAGTTCAACATCAGAGCATTCATACCAAAGAGAACTCATGAATGT
AATGAAGATGGGAAGATATTTATCAAATTCAGGCTTCATTCAGCATCTGAGAGTTCACACCAGGGAGAAATCATGTATGTACTGCATGTGGTAAAGCCTT
CAGTCATAGCTCAGCCATTGCTCAGCATCAGATAATTCACACCAGAGAGAAACCCTCTGAATGTGACGAATGAAGAAAAGGTATTAGTGTTAAACTCTTA
ATCGACTCCTGCAAATCTATACCAGTGAGAAATCTTACAAATGTATTGAATGTGGCAAATTTTTCATGCTATTAGTATTTTCATACCTTAGTCACATTTG
GAGAATTCACATGGGAATAAAATTCCATTGCTGCAATGAATGTGAAAAAGCCATCAGTCAAAGAAACTACCTTGTTTAGTATCAAATTCACGCCATGCAA
AAAGATTATAAATGTAATAAGCATGTATGTGTGTGAGGAGATTCAGTCATAACCCAACGCTCATTCAACATCAAAGAATTTATACCTAAGAGAACTTATT
TGGGTGTAGTAAATGGCAGATCTTTCAATAGGAGTTTAACTAGTCTTTGTCATATCAGAATATCCATAGTAGACAAGAATTTGATGTAACGCAAATGGAA
AAACTCGACACCACATTTCAGGCTTTACCCAACATCGAAATAATGGAGAGAAAATTGTTGATTATTTGTTTATGAAATTGTTAATACATAGTCCCAATCT
TTTTCATTGCACAAAAATCTAGGGTTGACTTGGTAAATGCAGTGACATTTTCTCATGGAGTTCCTTTATTTAATATGTATTCTAAGTAGGTACGTTTATT
TTTACTTTTTTATTATAATTTTGATATTAAAAAGAACAGAGATGGGGTCTTGCTTTGTTGCCCAGGCTGGTCTTGAACTCCTGGCCTCAAGCGATCCTCC
CACCTGTCCTCCCAAAGTGCTGGGGTTACAGGCGTGTGTCACTGTGCTGGGCCTATTTTATTTATAGAACTCATTTAAGCTGTTTTTATTTTAATATGCC
CTATAAACATTTTTATATTTTTTGAAATTGGTTCTTAGTGTTCACAACTTCCATAAGATACTGCTAATGCACCAGTATTAAAACACATCGACGTAAGTAG
CTCATTTAGCTTTTTCTGCTGTTCTTGGCCCAAGTTCTTTCCAAAACCAACTCTTAGGCCTGCTCTTTACTAGGGATCTTATGTCGTATTGCTTTACAGC
CACAACACTTGGATTCCTGTTGATTAACTTCTCCATTCTCTTAAGCACCTTTAGAAGATTTAGAAGTTTCCTAGTTTTAAGTGTTTCACCAGCAAGTATT
CCATACCTACTTGATGTTGCTGGTCTGGTGTCTTATTTCCTAAAGTGAAGCATCTTTTTTTAAAAAAGAATTTGATTGACAATATATCCAGTCCAATATA
AGTATGAAGGATTCTCTCTCCTGAGATTGTAGCAGGCAGCCAAACATTTTCAAATGATGCCCAAGGTTTTAGCTGTCTTGTGTGCATCCACAGTCTGCGA
AGAAGACATGATAAGGACATCAGGGAGCCAACAAGACTCCTAATAGCCTCACTACATTCATCCAGTGCCTATTCTGCATGCCTAAGCTTAGAGTTCTTTT
ATATACCTCTACGGCCAGCAAAATGCTCAGGTCTGCTCTTGGTAGGGTAAACATAAAGAAGATACACAGGCCGGGCATGGTGGCTCACACCTGTAATCCC
AGCACTTTGGGAGGCTGAGGCGGATGGATCACGAGGTCAGGCAGTCGAGACCATCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAGT
TAGCCGGGTGTGGTGGCACGCGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAACTGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCGAG
ATTGCACCACTGCACTCCAGCCTGGGCGACAGAGCAGGACTCTCTCTCAAAAAAACACAAAAAAACAAAAACAAAAAACCATACACACACACACACACAC
ACAAATCAGCATCATAAGGGAATGTAGCCTTCCAACAGAGATGATGCTGTTCGTATGTTAATCTCAGAGACAGTATTTCAAGAGAGTGGCAGGTCTGTTC
CTGGTAAAATTTTAACCATTAGGATTGCAGATAAATGTTTGAATTCTGCTCCTCTCTCATCAATCCAGGACAGTATTTGAAGTGTGAGGGCTTTGTGTAT
AGTTGTTTATCCATTACCACATTTTTGTATTTTAATAGTCTACAGGCTATATAAAAGAACATGGCTTTTTGACTGATAAAAGTGATTACAGATGTTGGCT
CAAGTTCAGGGCCACCATCATATACCTAACAAGAGTTCATGATTCTTTAGGTAATGTCAAAACATTTTGTATTTTTCCATCTTAAGCTTTATAACATTTT
GTGAGTAAGACAAATGTTATTTAAAATTCTTGTTGTCAGTCCAGCAATTGAGGCTTTCATAGTTCAGTGTTATAATATTCAGTAGGGACCCTCAACAAAT
ATATAAAAATATGTTGCTCACTCTATAATCCTCCTATGGCTAACCTCTAGGATAGTTCTGCCACTATATTTTACTTCTTTGCCATCAGCAAGAGTAGGAT
TTCATCAAGGCAAGGTAGGAATCTAAATGAAATTGATATATAAATGAATTGATCTAAATGTAAAAGCAAATGAAAAATGCATGTGTTTTTTCCTGTCAAA
CATGTATACCCTTATGTATAGAGACCAGTAGTCACGTATGGTGACTGAAACAGGATTATGTAATCCCTAAAAAGCAGAATATGTAAAAATCACATGTATG
CGTTTGGTTTAGGAATGTGCTTTTGTACTTCCACTTGAATAAAGGTGTGTTTGGTATTCTGAGAA
  • refseq_ZNF655.F1 (forward primer)
  • refseq_ZNF655.R1 (reverse primer)
  • Multiple exon skipping

Frame 0

WQLVLPSRE*KEAASGRGRR*FASPRVRGGPGA
RRACFSSALVHSPRGPRAPPSGAGDRALVRAVMV
IVLQSSDGGNTSPGSSRVTKGPVSVFGDPV*VS
VPRASVCAGHRHGTGTHWGWRDQRREQAVDS*A
ENFGRSAFIQSESRKTQTRYYPSS*D*RSV*V*G
QIRKTSGNSKEISVPGERV*ANNNQQGNLHQ*E
EQ*MS*TRKKLQSGLYY*CRSESS*NTEYR*Q*
*V*HELQPEFSLW*T*TLKSNRGFSE**M*GKLN
GSLPP**MGEHP*H*EIL*M*CMWENFPSELSP
Y*TSENPY*REALQM*RM*KVFQSELKS*ST*K
NTH*RKTLQM*SI**IL*SV**IL*SKLRHNSA*
ENSHQSQILQM*QL*KSLQS*CPPYSTSENSQR
DAL*VYCMWQ*LLPYFIPT*TSEGPS*RESL*V
**IWVGLY*TTRNSFQRKALYV**MWKRLQIEFT
SYSASKNSHRREST*M**MWKSFQSNLMPYSAS
QNA*EREIV*M**V*GQFQS*LRSYPATRSPHQ
TESL*L*CMGKELQSESTSSSTSEHSYQRELMNV
MKMGRYLSNSGFIQHLRVHTREKSCMYCMW*SL
QS*LSHCSASDNSHQRETL*M*RMKKRY*C*TL
NRLLQIYTSEKSYKCIECGKFFMLLVFSYLSHIW
RIHMGIKFHCCNECEKAISQRNYLV*YQIHAMQ
KDYKCNKHVCV*GDSVITQRSFNIKEFIPKRTY
LGVVNGRSFNRSLTSLCHIRISIVDKNLM*RKWK
NSTPHFRLYPTSK*WRENC*LFVYEIVNT*SQS
FSLHKNLGLTW*MQ*HFLMEFLYLICILSRYVY
FYFFIIILILKRTEMGSCFVAQAGLELLASSDPP
TCPPKVLGLQACVTVLGLFYL*NSFKLFLF*YA
L*TFLYFLKLVLSVHNFHKILLMHQY*NTST*V
AHLAFSAVLGPSSFQNQLLGLLFTRDLMSYCFTA
TTLGFLLINFSILLSTFRRFRSFLVLSVSPASI
PYLLDVAGLVSYFLK*SIFF*KRI*LTIYPVQY
KYEGFSLLRL*QAAKHFQMMPKVLAVLCASTVCE
EDMIRTSGSQQDS**PHYIHPVPILHA*A*SSF
IYLYGQQNAQVCSW*GKHKEDTQAGHGGSHL*S
QHFGRLRRMDHEVRQSRPSWPTW*NPVSTKNTKV
SRVWWHAPVIPATQEAEAGELLEPGRRRLQ*AE
IAPLHSSLGDRAGLSLKKTQKNKNKKPYTHTHT
HKSAS*GNVAFQQR*CCSYVNLRDSISREWQVCS
W*NFNH*DCR*MFEFCSSLINPGQYLKCEGFVY
SCLSITTFLYFNSLQAI*KNMAF*LIKVITDVG
SSSGPPSYT*QEFMIL*VMSKHFVFFHLKLYNIL
*VRQMLFKILVVSPAIEAFIVQCYNIQ*GPSTN
I*KYVAHSIILLWLTSRIVLPLYFTSLPSARVG
FHQGKVGI*MKLIYK*IDLNVKANEKCMCFFLSN
MYTLMYRDQ*SRMVTETGLCNP*KAEYVKITCM
RLV*ECAFVLPLE*RCVWYSE

Frame 1

GSLFCLHGSRRRRRPAAADGSSLPRECAEARVR
GGPVSLQPWFIHLADRGPRRQEPATVPWCEL*W
SLSSRAVMEEIPAQEAAGSPRVQFQSLETQSECL
SPEPQFVQDTDMEQGLTGDGETREENKLLIPKQ
KISEEVHSYKVRVGRLKHDITQVPETREVYKSE
DRLERLQEILRKFLYLEREFRQITISKETFTSEK
NNECHEPEKSFSLDSTIDADQRVLRIQNTDDND
KYDMSFNQNSASGKHEHLNLTEDFQSSECKESL
MDLSHLNKWESIPNTEKSYKCDVCGKIFHQSSAL
TRHQRIHTREKPYKCKECEKSFSQSSSLSRHKR
IHTREKPYKCEASDKSCEASDKSCSPSSGIIQH
KKIHTRAKSYKCSSCERVFSRSVHLTQHQKIHKE
MPCKCTVCGSDFCHTSYLLEHQRVHHEEKAYEY
DEYGLAYIKQQGIHFREKPYTCSECGKDFRLNS
HLIQHQRIHTGEKAHECNECGKAFSQTSCLIQHH
KMHRKEKSYECNEYEGSFSHSSDLILQQEVLTR
QKAFDCDVWEKNSSQRAHLVQHQSIHTKENS*M
**RWEDIYQIQASFSI*EFTPGRNHVCTACGKAF
SHSSAIAQHQIIHTREKPSECDE*RKGISVKLL
IDSCKSIPVRNLTNVLNVANFSCY*YFHTLVTF
GEFTWE*NSIAAMNVKKPSVKETTLFSIKFTPCK
KIINVISMYVCEEIQS*PNAHSTSKNLYLRELI
WV**MADLSIGV*LVFVISEYP**TRI*CNANG
KTRHHISGFTQHRNNGEKIVDYLFMKLLIHSPNL
FHCTKI*G*LGKCSDIFSWSSFI*YVF*VGTFI
FTFLL*F*Y*KEQRWGLALLPRLVLNSWPQAIL
PPVLPKCWGYRRVSLCWAYFIYRTHLSCFYFNMP
YKHFYIF*NWFLVFTTSIRYC*CTSIKTHRRK*
LI*LFLLFLAQVLSKTNS*ACSLLGILCRIALQ
PQHLDSC*LTSPFS*APLEDLEVS*F*VFHQQVF
HTYLMLLVWCLIS*SEASFFKKEFD*QYIQSNI
SMKDSLS*DCSRQPNIFK*CPRF*LSCVHPQSA
KKT**GHQGANKTPNSLTTFIQCLFCMPKLRVLL
YTSTASKMLRSALGRVNIKKIHRPGMVAHTCNP
STLGG*GGWITRSGSRDHPGQHGETPSLLKIQK
LAGCGGTRL*SQLLRRLRQENCLNLGGGGCSEPR
LHHCTPAWATEQDSLSKKHKKTKTKNHTHTHTH
TNQHHKGM*PSNRDDAVRMLISETVFQESGRSV
PGKILTIRIADKCLNSAPLSSIQDSI*SVRALCI
VVYPLPHFCILIVYRLYKRTWLFD**K*LQMLA
QVQGHHHIPNKSS*FFR*CQNILYFSILSFITF
CE*DKCYLKFLLSVQQLRLS*FSVIIFSRDPQQI
YKNMLLTL*SSYG*PLG*FCHYILLLCHQQE*D
FIKAR*ESK*N*YINELI*M*KQMKNACVFSCQ
TCIPLCIETSSHVW*LKQDYVIPKKQNM*KSHVC
VWFRNVLLYFHLNKGVFGILR

Frame 2

AACFAFTGVEGGGVRPRPTVVRFPESARRPGCE
EGLFLFSPGSFTSRTEGPAVRSRRPCPGASCDG
HCPPEQ*WRKYQPRKQQGHQGSSFSLWRPSLSV
CPQSLSLCRTPTWNRDSLGMERPEKRTSC*FLSR
KFRKKCIHTK*E*EDSNTILPKFLRLEKCISLR
TD*KDFRKF*GNFCTWRESLGK*QSARKPSPVR
RTMNVMNPKKASVWTLLLMQIREFLEYRIPMTMI
SMT*ASTRIQPLVNMNT*I*QRIFRVVNVRKA*
WISPTLINGRASLTLRNPINVMYVGKFSIRAQP
LLDIRESILERSPTNVKNVKSLSVRAQVLVDIKE
YTLEKNLTNVKHLINPVKRLINPVVQAQA*FSI
RKFTPEPNLTNVAVVKESSVVVSTLLNIRKFTK
RCPVSVLYVAVTSAILHTYLNIRGSIMKRKPMSM
MNMGWPILNNKEFISEKSPIRVVNVEKTSD*IH
ILFSIKEFTQERKHMNVMNVEKLSVKPHALFSI
TKCIGKRNRMNVMSMRAVSVIAQILSCNKKSSPD
RKPLIVMYGKRTPVREHI*FNIRAFIPKRTHEC
NEDGKIFIKFRLHSASESSHQGEIMYVLHVVKP
SVIAQPLLSIR*FTPERNPLNVTNEEKVLVLNS*
STPANLYQ*EILQMY*MWQIFHAISIFIP*SHL
ENSHGNKIPLLQ*M*KSHQSKKLPCLVSNSRHA
KRL*M**ACMCVRRFSHNPTLIQHQRIYT*ENLF
GCSKWQIFQ*EFN*SLSYQNIHSRQEFDVTQME
KLDTTFQALPNIEIMERKLLIICL*NC*YIVPI
FFIAQKSRVDLVNAVTFSHGVPLFNMYSK*VRLF
LLFYYNFDIKKNRDGVLLCCPGWS*TPGLKRSS
HLSSQSAGVTGVCHCAGPILFIELI*AVFILIC
PINIFIFFEIGS*CSQLP*DTANAPVLKHIDVSS
SFSFFCCSWPKFFPKPTLRPALY*GSYVVLLYS
HNTWIPVD*LLHSLKHL*KI*KFPSFKCFTSKY
SIPT*CCWSGVLFPKVKHLFLKKNLIDNISSPI*
V*RILSPEIVAGSQTFSNDAQGFSCLVCIHSLR
RRHDKDIREPTRLLIASLHSSSAYSACLSLEFF
YIPLRPAKCSGLLLVG*T*RRYTGRAWWLTPVIP
ALWEAEADGSRGQAVETILANMVKPRLY*KYKS
*PGVVARACNPSYSGG*GRRTA*TWEAEVAVSR
DCTTALQPGRQSRTLSQKNTKKQKQKTIHTHTHT
QISIIRECSLPTEMMLFVC*SQRQYFKRVAGLF
LVKF*PLGLQINV*ILLLSHQSRTVFEV*GLCV
*LFIHYHIFVF**STGYIKEHGFLTDKSDYRCWL
KFRATIIYLTRVHDSLGNVKTFCIFPS*AL*HF
VSKTNVI*NSCCQSSN*GFHSSVL*YSVGTLNK
YIKICCSLYNPPMANL*DSSATIFYFFAISKSRI
SSRQGRNLNEIDI*MN*SKCKSK*KMHVFFPVK
HVYPYV*RPVVTYGD*NRIM*SLKSRICKNHMY
AFGLGMCFCTST*IKVCLVF*E

Sequence NM_138494

CAGTGAGTTAGCATGGAGGGCAGTGGGACCGGAAAAAGACGTGGAAAAGCTGCGAAAACGAGCCTTCGAATCATGGACGCGCGGGCCCAGCTCCTCCTCC
GAGTTCCTCATCCGGGGCCGTCACTCACATCCGGGGCCCTCACTCACATCCGGGACCCTCATCCGGGGCTCTCACCCACATCCGGGACCCTCATGCCTGG
GCGGAGGAGGGGGGGCCCTCATTCGGGACCCTGCACTCCGTCGCCGGAAGTGCCACCGAGAAGCGCCGGCCTCGGGGCTGTCTACAGCGGCCCGGGAGAG
GCTGTGGTGGCCCCGAGCGCGAGTGTGTAGGTGACAGGACAGCGGCCAGGCCCGCCCCTCCCCTCGTGATGGTCATTGTCCTCCAGAGCAGTGATGGAGG
AAATACCAGCCCAGGAAGCAGCAGGGTCACCAAGGGTCCAGTTTCAGTCTTTGGAGACCCAGTCTGAGTGTCTGTCCCCAGAGCCTCAGTTTGTGCAGGA
CACCGACATGGAACAGGGACTCACTGGGGATGGAGAGACCAGAGAAGAGAACAAGCTGTTGATTCCTAAGCAGAAAATTTCGGAAGAAGTGCATTCATAC
AAAGTGAGAGTAGGAAGACTCAAACACGATATTACCCAAGTTCCTGAGACTAGAGAAGTGTATAAGTCTGAGGACAGATTAGAAAGACTTCAGGAAATTC
TAAGGAAATTTCTGTACCTGGAGAGAGAGTTTAGGCAAATAACAATCAGCAAGGAAACCTTCACCAGTGAGAAGAACAATGAATGTCATGAACCCGAAAA
AAGCTTCAGTCTGGACTCTACTATTGATGCAGATCAGAGAGTTCTTAGAATACAGAATACCGATGACAATGATAAGTATGACATGAGCTTCAACCAGAAT
TCAGCCTCTGGTAAACATGAACACTTAAATCTAACAGAGGATTTTCAGAGTAGTGAATGTAAGGAAAGCTTAATGGATCTCTCCCACCTTAATAAATGGG
AGAGCATCCCTAACACTGAGAAATCCTATAAATGTGATGTATGTGGGAAAATTTTCCATCAGAGCTCAGCCCTTACTAGACATCAGAGAATCCATACTAG
AGAGAAGCCCTACAAATGTAAAGAATGTGAAAAGTCTTTCAGTCAGAGCTCAAGTCTTAGTCGACATAAAAGAATACACACTAGAGAAAAACCTTACAAA
TGTGAAGCATCTGATAAATCCTGTGAAGCGTCTGATAAATCCTGTAGTCCAAGCTCAGGCATAATTCAGCATAAGAAAATTCACACCAGAGCCAAATCTT
ACAAATGTAGCAGTTGTGAAAGAGTCTTCAGTCGTAGTGTCCACCTTACTCAACATCAGAAAATTCACAAAGAGATGCCCTGTAAGTGTACTGTATGTGG
CAGTGACTTCTGCCATACTTCATACCTACTTGAACATCAGAGGGTCCATCATGAAGAGAAAGCCTATGAGTATGATGAATATGGGTTGGCCTATATTAAA
CAACAAGGAATTCATTTCAGAGAAAAGCCCTATACGTGTAGTGAATGTGGAAAAGACTTCAGATTGAATTCACATCTTATTCAGCATCAAAGAATTCACA
CAGGAGAGAAAGCACATGAATGTAATGAATGTGGAAAAGCTTTCAGTCAAACCTCATGCCTTATTCAGCATCACAAAATGCATAGGAAAGAGAAATCGTA
TGAATGTAATGAGTATGAGGGCAGTTTCAGTCATAGCTCAGATCTTATCCTGCAACAAGAAGTCCTCACCAGACAGAAAGCCTTTGATTGTGATGTATGG
GAAAAGAACTCCAGTCAGAGAGCACATCTAGTTCAACATCAGAGCATTCATACCAAAGAGAACTCATGAATGTAATGAAGATGGGAAGATATTTATCAAA
TTCAGGCTTCATTCAGCATCTGAGAGTTCACACCAGGGAGAAATCATGTATGTACTGCATGTGGTAAAGCCTTCAGTCATAGCTCAGCCATTGCTCAGCA
TCAGATAATTCACACCAGAGAGAAACCCTCTGAATGTGACGAATGAAGAAAAGGTATTAGTGTTAAACTCTTAATCGACTCCTGCAAATCTATACCAGTG
AGAAATCTTACAAATGTATTGAATGTGGCAAATTTTTCATGCTATTAGTATTTTCATACCTTAGTCACATTTGGAGAATTCACATGGGAATAAAATTCCA
TTGCTGCAATGAATGTGAAAAAGCCATCAGTCAAAGAAACTACCTTGTTTAGTATCAAATTCACGCCATGCAAAAAGATTATAAATGTAATAAGCATGTA
TGTGTGTGAGGAGATTCAGTCATAACCCAACGCTCATTCAACATCAAAGAATTTATACCTAAGAGAACTTATTTGGGTGTAGTAAATGGCAGATCTTTCA
ATAGGAGTTTAACTAGTCTTTGTCATATCAGAATATCCATAGTAGACAAGAATTTGATGTAACGCAAATGGAAAAACTCGACACCACATTTCAGGCTTTA
CCCAACATCGAAATAATGGAGAGAAAATTGTTGATTATTTGTTTATGAAATTGTTAATACATAGTCCCAATCTTTTTCATTGCACAAAAATCTAGGGTTG
ACTTGGTAAATGCAGTGACATTTTCTCATGGAGTTCCTTTATTTAATATGTATTCTAAGTAGGTACGTTTATTTTTACTTTTTTATTATAATTTTGATAT
TAAAAAGAACAGAGATGGGGTCTTGCTTTGTTGCCCAGGCTGGTCTTGAACTCCTGGCCTCAAGCGATCCTCCCACCTGTCCTCCCAAAGTGCTGGGGTT
ACAGGCGTGTGTCACTGTGCTGGGCCTATTTTATTTATAGAACTCATTTAAGCTGTTTTTATTTTAATATGCCCTATAAACATTTTTATATTTTTTGAAA
TTGGTTCTTAGTGTTCACAACTTCCATAAGATACTGCTAATGCACCAGTATTAAAACACATCGACGTAAGTAGCTCATTTAGCTTTTTCTGCTGTTCTTG
GCCCAAGTTCTTTCCAAAACCAACTCTTAGGCCTGCTCTTTACTAGGGATCTTATGTCGTATTGCTTTACAGCCACAACACTTGGATTCCTGTTGATTAA
CTTCTCCATTCTCTTAAGCACCTTTAGAAGATTTAGAAGTTTCCTAGTTTTAAGTGTTTCACCAGCAAGTATTCCATACCTACTTGATGTTGCTGGTCTG
GTGTCTTATTTCCTAAAGTGAAGCATCTTTTTTTAAAAAAGAATTTGATTGACAATATATCCAGTCCAATATAAGTATGAAGGATTCTCTCTCCTGAGAT
TGTAGCAGGCAGCCAAACATTTTCAAATGATGCCCAAGGTTTTAGCTGTCTTGTGTGCATCCACAGTCTGCGAAGAAGACATGATAAGGACATCAGGGAG
CCAACAAGACTCCTAATAGCCTCACTACATTCATCCAGTGCCTATTCTGCATGCCTAAGCTTAGAGTTCTTTTATATACCTCTACGGCCAGCAAAATGCT
CAGGTCTGCTCTTGGTAGGGTAAACATAAAGAAGATACACAGGCCGGGCATGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGATGG
ATCACGAGGTCAGGCAGTCGAGACCATCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAGTTAGCCGGGTGTGGTGGCACGCGCCTGT
AATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAACTGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCGAGATTGCACCACTGCACTCCAGCCTGGGC
GACAGAGCAGGACTCTCTCTCAAAAAAACACAAAAAAACAAAAACAAAAAACCATACACACACACACACACACACAAATCAGCATCATAAGGGAATGTAG
CCTTCCAACAGAGATGATGCTGTTCGTATGTTAATCTCAGAGACAGTATTTCAAGAGAGTGGCAGGTCTGTTCCTGGTAAAATTTTAACCATTAGGATTG
CAGATAAATGTTTGAATTCTGCTCCTCTCTCATCAATCCAGGACAGTATTTGAAGTGTGAGGGCTTTGTGTATAGTTGTTTATCCATTACCACATTTTTG
TATTTTAATAGTCTACAGGCTATATAAAAGAACATGGCTTTTTGACTGATAAAAGTGATTACAGATGTTGGCTCAAGTTCAGGGCCACCATCATATACCT
AACAAGAGTTCATGATTCTTTAGGTAATGTCAAAACATTTTGTATTTTTCCATCTTAAGCTTTATAACATTTTGTGAGTAAGACAAATGTTATTTAAAAT
TCTTGTTGTCAGTCCAGCAATTGAGGCTTTCATAGTTCAGTGTTATAATATTCAGTAGGGACCCTCAACAAATATATAAAAATATGTTGCTCACTCTATA
ATCCTCCTATGGCTAACCTCTAGGATAGTTCTGCCACTATATTTTACTTCTTTGCCATCAGCAAGAGTAGGATTTCATCAAGGCAAGGTAGGAATCTAAA
TGAAATTGATATATAAATGAATTGATCTAAATGTAAAAGCAAATGAAAAATGCATGTGTTTTTTCCTGTCAAACATGTATACCCTTATGTATAGAGACCA
GTAGTCACGTATGGTGACTGAAACAGGATTATGTAATCCCTAAAAAGCAGAATATGTAAAAATCACATGTATGCGTTTGGTTTAGGAATGTGCTTTTGTA
CTTCCACTTGAATAAAGGTGTGTTTGGTATTCTGAGAA
  • refseq_ZNF655.F1 (forward primer)
  • refseq_ZNF655.R1 (reverse primer)
  • Multiple exon skipping

Frame 0

Q*VSMEGSGTGKRRGKAAKTSLRIMDARAQLLL
RVPHPGPSLTSGALTHIRDPHPGLSPTSGTLMPG
RRRGGPHSGPCTPSPEVPPRSAGLGAVYSGPGE
AVVAPSASV*VTGQRPGPPLPS*WSLSSRAVME
EIPAQEAAGSPRVQFQSLETQSECLSPEPQFVQD
TDMEQGLTGDGETREENKLLIPKQKISEEVHSY
KVRVGRLKHDITQVPETREVYKSEDRLERLQEI
LRKFLYLEREFRQITISKETFTSEKNNECHEPEK
SFSLDSTIDADQRVLRIQNTDDNDKYDMSFNQN
SASGKHEHLNLTEDFQSSECKESLMDLSHLNKW
ESIPNTEKSYKCDVCGKIFHQSSALTRHQRIHTR
EKPYKCKECEKSFSQSSSLSRHKRIHTREKPYK
CEASDKSCEASDKSCSPSSGIIQHKKIHTRAKS
YKCSSCERVFSRSVHLTQHQKIHKEMPCKCTVCG
SDFCHTSYLLEHQRVHHEEKAYEYDEYGLAYIK
QQGIHFREKPYTCSECGKDFRLNSHLIQHQRIH
TGEKAHECNECGKAFSQTSCLIQHHKMHRKEKSY
ECNEYEGSFSHSSDLILQQEVLTRQKAFDCDVW
EKNSSQRAHLVQHQSIHTKENS*M**RWEDIYQ
IQASFSI*EFTPGRNHVCTACGKAFSHSSAIAQH
QIIHTREKPSECDE*RKGISVKLLIDSCKSIPV
RNLTNVLNVANFSCY*YFHTLVTFGEFTWE*NS
IAAMNVKKPSVKETTLFSIKFTPCKKIINVISMY
VCEEIQS*PNAHSTSKNLYLRELIWV**MADLS
IGV*LVFVISEYP**TRI*CNANGKTRHHISGF
TQHRNNGEKIVDYLFMKLLIHSPNLFHCTKI*G*
LGKCSDIFSWSSFI*YVF*VGTFIFTFLL*F*Y
*KEQRWGLALLPRLVLNSWPQAILPPVLPKCWG
YRRVSLCWAYFIYRTHLSCFYFNMPYKHFYIF*N
WFLVFTTSIRYC*CTSIKTHRRK*LI*LFLLFL
AQVLSKTNS*ACSLLGILCRIALQPQHLDSC*L
TSPFS*APLEDLEVS*F*VFHQQVFHTYLMLLVW
CLIS*SEASFFKKEFD*QYIQSNISMKDSLS*D
CSRQPNIFK*CPRF*LSCVHPQSAKKT**GHQG
ANKTPNSLTTFIQCLFCMPKLRVLLYTSTASKML
RSALGRVNIKKIHRPGMVAHTCNPSTLGG*GGW
ITRSGSRDHPGQHGETPSLLKIQKLAGCGGTRL
*SQLLRRLRQENCLNLGGGGCSEPRLHHCTPAWA
TEQDSLSKKHKKTKTKNHTHTHTHTNQHHKGM*
PSNRDDAVRMLISETVFQESGRSVPGKILTIRI
ADKCLNSAPLSSIQDSI*SVRALCIVVYPLPHFC
ILIVYRLYKRTWLFD**K*LQMLAQVQGHHHIP
NKSS*FFR*CQNILYFSILSFITFCE*DKCYLK
FLLSVQQLRLS*FSVIIFSRDPQQIYKNMLLTL*
SSYG*PLG*FCHYILLLCHQQE*DFIKAR*ESK
*N*YINELI*M*KQMKNACVFSCQTCIPLCIET
SSHVW*LKQDYVIPKKQNM*KSHVCVWFRNVLLY
FHLNKGVFGILR

Frame 1

SELAWRAVGPEKDVEKLRKRAFESWTRGPSSSS
EFLIRGRHSHPGPSLTSGTLIRGSHPHPGPSCL
GGGGGALIRDPALRRRKCHREAPASGLSTAARER
LWWPRARVCR*QDSGQARPSPRDGHCPPEQ*WR
KYQPRKQQGHQGSSFSLWRPSLSVCPQSLSLCR
TPTWNRDSLGMERPEKRTSC*FLSRKFRKKCIHT
K*E*EDSNTILPKFLRLEKCISLRTD*KDFRKF
*GNFCTWRESLGK*QSARKPSPVRRTMNVMNPK
KASVWTLLLMQIREFLEYRIPMTMISMT*ASTRI
QPLVNMNT*I*QRIFRVVNVRKA*WISPTLING
RASLTLRNPINVMYVGKFSIRAQPLLDIRESIL
ERSPTNVKNVKSLSVRAQVLVDIKEYTLEKNLTN
VKHLINPVKRLINPVVQAQA*FSIRKFTPEPNL
TNVAVVKESSVVVSTLLNIRKFTKRCPVSVLYV
AVTSAILHTYLNIRGSIMKRKPMSMMNMGWPILN
NKEFISEKSPIRVVNVEKTSD*IHILFSIKEFT
QERKHMNVMNVEKLSVKPHALFSITKCIGKRNR
MNVMSMRAVSVIAQILSCNKKSSPDRKPLIVMYG
KRTPVREHI*FNIRAFIPKRTHECNEDGKIFIK
FRLHSASESSHQGEIMYVLHVVKPSVIAQPLLS
IR*FTPERNPLNVTNEEKVLVLNS*STPANLYQ*
EILQMY*MWQIFHAISIFIP*SHLENSHGNKIP
LLQ*M*KSHQSKKLPCLVSNSRHAKRL*M**AC
MCVRRFSHNPTLIQHQRIYT*ENLFGCSKWQIFQ
*EFN*SLSYQNIHSRQEFDVTQMEKLDTTFQAL
PNIEIMERKLLIICL*NC*YIVPIFFIAQKSRV
DLVNAVTFSHGVPLFNMYSK*VRLFLLFYYNFDI
KKNRDGVLLCCPGWS*TPGLKRSSHLSSQSAGV
TGVCHCAGPILFIELI*AVFILICPINIFIFFE
IGS*CSQLP*DTANAPVLKHIDVSSSFSFFCCSW
PKFFPKPTLRPALY*GSYVVLLYSHNTWIPVD*
LLHSLKHL*KI*KFPSFKCFTSKYSIPT*CCWS
GVLFPKVKHLFLKKNLIDNISSPI*V*RILSPEI
VAGSQTFSNDAQGFSCLVCIHSLRRRHDKDIRE
PTRLLIASLHSSSAYSACLSLEFFYIPLRPAKC
SGLLLVG*T*RRYTGRAWWLTPVIPALWEAEADG
SRGQAVETILANMVKPRLY*KYKS*PGVVARAC
NPSYSGG*GRRTA*TWEAEVAVSRDCTTALQPG
RQSRTLSQKNTKKQKQKTIHTHTHTQISIIRECS
LPTEMMLFVC*SQRQYFKRVAGLFLVKF*PLGL
QINV*ILLLSHQSRTVFEV*GLCV*LFIHYHIF
VF**STGYIKEHGFLTDKSDYRCWLKFRATIIYL
TRVHDSLGNVKTFCIFPS*AL*HFVSKTNVI*N
SCCQSSN*GFHSSVL*YSVGTLNKYIKICCSLY
NPPMANL*DSSATIFYFFAISKSRISSRQGRNLN
EIDI*MN*SKCKSK*KMHVFFPVKHVYPYV*RP
VVTYGD*NRIM*SLKSRICKNHMYAFGLGMCFC
TST*IKVCLVF*E

Frame 2

VS*HGGQWDRKKTWKSCENEPSNHGRAGPAPPP
SSSSGAVTHIRGPHSHPGPSSGALTHIRDPHAW
AEEGGPSFGTLHSVAGSATEKRRPRGCLQRPGR
GCGGPERECVGDRTAARPAPPLVMVIVLQSSDGG
NTSPGSSRVTKGPVSVFGDPV*VSVPRASVCAG
HRHGTGTHWGWRDQRREQAVDS*AENFGRSAFI
QSESRKTQTRYYPSS*D*RSV*V*GQIRKTSGNS
KEISVPGERV*ANNNQQGNLHQ*EEQ*MS*TRK
KLQSGLYY*CRSESS*NTEYR*Q**V*HELQPE
FSLW*T*TLKSNRGFSE**M*GKLNGSLPP**MG
EHP*H*EIL*M*CMWENFPSELSPY*TSENPY*
REALQM*RM*KVFQSELKS*ST*KNTH*RKTLQ
M*SI**IL*SV**IL*SKLRHNSA*ENSHQSQIL
QM*QL*KSLQS*CPPYSTSENSQRDAL*VYCMW
Q*LLPYFIPT*TSEGPS*RESL*V**IWVGLY*
TTRNSFQRKALYV**MWKRLQIEFTSYSASKNSH
RREST*M**MWKSFQSNLMPYSASQNA*EREIV
*M**V*GQFQS*LRSYPATRSPHQTESL*L*CM
GKELQSESTSSSTSEHSYQRELMNVMKMGRYLSN
SGFIQHLRVHTREKSCMYCMW*SLQS*LSHCSA
SDNSHQRETL*M*RMKKRY*C*TLNRLLQIYTS
EKSYKCIECGKFFMLLVFSYLSHIWRIHMGIKFH
CCNECEKAISQRNYLV*YQIHAMQKDYKCNKHV
CV*GDSVITQRSFNIKEFIPKRTYLGVVNGRSF
NRSLTSLCHIRISIVDKNLM*RKWKNSTPHFRLY
PTSK*WRENC*LFVYEIVNT*SQSFSLHKNLGL
TW*MQ*HFLMEFLYLICILSRYVYFYFFIIILI
LKRTEMGSCFVAQAGLELLASSDPPTCPPKVLGL
QACVTVLGLFYL*NSFKLFLF*YAL*TFLYFLK
LVLSVHNFHKILLMHQY*NTST*VAHLAFSAVL
GPSSFQNQLLGLLFTRDLMSYCFTATTLGFLLIN
FSILLSTFRRFRSFLVLSVSPASIPYLLDVAGL
VSYFLK*SIFF*KRI*LTIYPVQYKYEGFSLLR
L*QAAKHFQMMPKVLAVLCASTVCEEDMIRTSGS
QQDS**PHYIHPVPILHA*A*SSFIYLYGQQNA
QVCSW*GKHKEDTQAGHGGSHL*SQHFGRLRRM
DHEVRQSRPSWPTW*NPVSTKNTKVSRVWWHAPV
IPATQEAEAGELLEPGRRRLQ*AEIAPLHSSLG
DRAGLSLKKTQKNKNKKPYTHTHTHKSAS*GNV
AFQQR*CCSYVNLRDSISREWQVCSW*NFNH*DC
R*MFEFCSSLINPGQYLKCEGFVYSCLSITTFL
YFNSLQAI*KNMAF*LIKVITDVGSSSGPPSYT
*QEFMIL*VMSKHFVFFHLKLYNIL*VRQMLFKI
LVVSPAIEAFIVQCYNIQ*GPSTNI*KYVAHSI
ILLWLTSRIVLPLYFTSLPSARVGFHQGKVGI*
MKLIYK*IDLNVKANEKCMCFFLSNMYTLMYRDQ
*SRMVTETGLCNP*KAEYVKITCMRLV*ECAFV
LPLE*RCVWYSE

Sequence NM_001009956

CAGTGAGTTAGCATGGAGGGCAGTGGGACCGGAAAAAGACGTGGAAAAGCTGCGAAAACGAGCCTTCGAATCATGGACGCGCGGGCCCAGCTCCTCCTCC
GAGTTCCTCATCCGGGGCCGTCACTCACATCCGGGGCCCTCACTCACATCCGGGACCCTCATCCGGGGCTCTCACCCACATCCGGGACCCTCATGCCTGG
GCGGAGGAGGGGGGGCCCTCATTCGGGACCCTGCACTCCGTCGCCGGAAGTGCCACCGAGAAGCGCCGGCCTCGGGGCTGTCTACAGCGGCCCGGGAGAG
GCTGTGGTGGCCCCGAGCGCGAGTGTGTAGGTGACAGGACAGCGGCCAGGCCCGCCCCTCCCCTCGTGATGGTCATTGTCCTCCAGAGCAGTGATGGAGG
AAATACCAGCCCAGGAAGCAGCAGGGTCACCAAGGGTCCAGTTTCAGTCTTTGGAGACCCAGTCTGAGTGTCTGTCCCCAGAGCCTCAGTTTGTGCAGGA
CACCGACATGGAACAGGGACTCACTGGGGGTTAGCTAACCATGCAGCAGGTGCTTGAGTTTCACCTTTCTCCAGTCCAGCAGAATTACATTTTGATGACC
TCCATTTTGATACTTGTAGACCAGATCTAATCCGAAGATTAATCTTTAGGATTTCCAATTTCCAAGCCTGATGGGATCTCCCAGCTGGAACAGGATCTAC
AGGTCTTTGATCTGGAAACTAAGACTAGAGAAGTCTTAAGAGATGACTGCTCAGATGGAGAGACCAGAGAAGAGAACAAGCTGTTGATTCCTAAGCAGAA
AATTTCGGAAGAAGTGCATTCATACAAAGTGAGAGTAGGAAGACTCAAACACGATATTACCCAAGTTCCTGAGACTAGAGAAGTGTATAAGTCTGAGGAC
AGATTAGAAAGACTTCAGGAAATTCTAAGGAAATTTCTGTACCTGGAGAGAGAGTTTAGGCAAATAACAATCAGCAAGGAAACCTTCACCAGTGAGAAGA
ACAATGAATGTCATGAACCCGAAAAAAGCTTCAGTCTGGACTCTACTATTGATGCAGATCAGAGAGTTCTTAGAATACAGAATACCGATGACAATGATAA
GTATGACATGAGCTTCAACCAGAATTCAGCCTCTGGTAAACATGAACACTTAAATCTAACAGAGGATTTTCAGAGTAGTGAATGTAAGGAAAGCTTAATG
GATCTCTCCCACCTTAATAAATGGGAGAGCATCCCTAACACTGAGAAATCCTATAAATGTGATGTATGTGGGAAAATTTTCCATCAGAGCTCAGCCCTTA
CTAGACATCAGAGAATCCATACTAGAGAGAAGCCCTACAAATGTAAAGAATGTGAAAAGTCTTTCAGTCAGAGCTCAAGTCTTAGTCGACATAAAAGAAT
ACACACTAGAGAAAAACCTTACAAATGTGAAGCATCTGATAAATCCTGTGAAGCGTCTGATAAATCCTGTAGTCCAAGCTCAGGCATAATTCAGCATAAG
AAAATTCACACCAGAGCCAAATCTTACAAATGTAGCAGTTGTGAAAGAGTCTTCAGTCGTAGTGTCCACCTTACTCAACATCAGAAAATTCACAAAGAGA
TGCCCTGTAAGTGTACTGTATGTGGCAGTGACTTCTGCCATACTTCATACCTACTTGAACATCAGAGGGTCCATCATGAAGAGAAAGCCTATGAGTATGA
TGAATATGGGTTGGCCTATATTAAACAACAAGGAATTCATTTCAGAGAAAAGCCCTATACGTGTAGTGAATGTGGAAAAGACTTCAGATTGAATTCACAT
CTTATTCAGCATCAAAGAATTCACACAGGAGAGAAAGCACATGAATGTAATGAATGTGGAAAAGCTTTCAGTCAAACCTCATGCCTTATTCAGCATCACA
AAATGCATAGGAAAGAGAAATCGTATGAATGTAATGAGTATGAGGGCAGTTTCAGTCATAGCTCAGATCTTATCCTGCAACAAGAAGTCCTCACCAGACA
GAAAGCCTTTGATTGTGATGTATGGGAAAAGAACTCCAGTCAGAGAGCACATCTAGTTCAACATCAGAGCATTCATACCAAAGAGAACTCATGAATGTAA
TGAAGATGGGAAGATATTTATCAAATTCAGGCTTCATTCAGCATCTGAGAGTTCACACCAGGGAGAAATCATGTATGTACTGCATGTGGTAAAGCCTTCA
GTCATAGCTCAGCCATTGCTCAGCATCAGATAATTCACACCAGAGAGAAACCCTCTGAATGTGACGAATGAAGAAAAGGTATTAGTGTTAAACTCTTAAT
CGACTCCTGCAAATCTATACCAGTGAGAAATCTTACAAATGTATTGAATGTGGCAAATTTTTCATGCTATTAGTATTTTCATACCTTAGTCACATTTGGA
GAATTCACATGGGAATAAAATTCCATTGCTGCAATGAATGTGAAAAAGCCATCAGTCAAAGAAACTACCTTGTTTAGTATCAAATTCACGCCATGCAAAA
AGATTATAAATGTAATAAGCATGTATGTGTGTGAGGAGATTCAGTCATAACCCAACGCTCATTCAACATCAAAGAATTTATACCTAAGAGAACTTATTTG
GGTGTAGTAAATGGCAGATCTTTCAATAGGAGTTTAACTAGTCTTTGTCATATCAGAATATCCATAGTAGACAAGAATTTGATGTAACGCAAATGGAAAA
ACTCGACACCACATTTCAGGCTTTACCCAACATCGAAATAATGGAGAGAAAATTGTTGATTATTTGTTTATGAAATTGTTAATACATAGTCCCAATCTTT
TTCATTGCACAAAAATCTAGGGTTGACTTGGTAAATGCAGTGACATTTTCTCATGGAGTTCCTTTATTTAATATGTATTCTAAGTAGGTACGTTTATTTT
TACTTTTTTATTATAATTTTGATATTAAAAAGAACAGAGATGGGGTCTTGCTTTGTTGCCCAGGCTGGTCTTGAACTCCTGGCCTCAAGCGATCCTCCCA
CCTGTCCTCCCAAAGTGCTGGGGTTACAGGCGTGTGTCACTGTGCTGGGCCTATTTTATTTATAGAACTCATTTAAGCTGTTTTTATTTTAATATGCCCT
ATAAACATTTTTATATTTTTTGAAATTGGTTCTTAGTGTTCACAACTTCCATAAGATACTGCTAATGCACCAGTATTAAAACACATCGACGTAAGTAGCT
CATTTAGCTTTTTCTGCTGTTCTTGGCCCAAGTTCTTTCCAAAACCAACTCTTAGGCCTGCTCTTTACTAGGGATCTTATGTCGTATTGCTTTACAGCCA
CAACACTTGGATTCCTGTTGATTAACTTCTCCATTCTCTTAAGCACCTTTAGAAGATTTAGAAGTTTCCTAGTTTTAAGTGTTTCACCAGCAAGTATTCC
ATACCTACTTGATGTTGCTGGTCTGGTGTCTTATTTCCTAAAGTGAAGCATCTTTTTTTAAAAAAGAATTTGATTGACAATATATCCAGTCCAATATAAG
TATGAAGGATTCTCTCTCCTGAGATTGTAGCAGGCAGCCAAACATTTTCAAATGATGCCCAAGGTTTTAGCTGTCTTGTGTGCATCCACAGTCTGCGAAG
AAGACATGATAAGGACATCAGGGAGCCAACAAGACTCCTAATAGCCTCACTACATTCATCCAGTGCCTATTCTGCATGCCTAAGCTTAGAGTTCTTTTAT
ATACCTCTACGGCCAGCAAAATGCTCAGGTCTGCTCTTGGTAGGGTAAACATAAAGAAGATACACAGGCCGGGCATGGTGGCTCACACCTGTAATCCCAG
CACTTTGGGAGGCTGAGGCGGATGGATCACGAGGTCAGGCAGTCGAGACCATCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAGTTA
GCCGGGTGTGGTGGCACGCGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAACTGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCGAGAT
TGCACCACTGCACTCCAGCCTGGGCGACAGAGCAGGACTCTCTCTCAAAAAAACACAAAAAAACAAAAACAAAAAACCATACACACACACACACACACAC
AAATCAGCATCATAAGGGAATGTAGCCTTCCAACAGAGATGATGCTGTTCGTATGTTAATCTCAGAGACAGTATTTCAAGAGAGTGGCAGGTCTGTTCCT
GGTAAAATTTTAACCATTAGGATTGCAGATAAATGTTTGAATTCTGCTCCTCTCTCATCAATCCAGGACAGTATTTGAAGTGTGAGGGCTTTGTGTATAG
TTGTTTATCCATTACCACATTTTTGTATTTTAATAGTCTACAGGCTATATAAAAGAACATGGCTTTTTGACTGATAAAAGTGATTACAGATGTTGGCTCA
AGTTCAGGGCCACCATCATATACCTAACAAGAGTTCATGATTCTTTAGGTAATGTCAAAACATTTTGTATTTTTCCATCTTAAGCTTTATAACATTTTGT
GAGTAAGACAAATGTTATTTAAAATTCTTGTTGTCAGTCCAGCAATTGAGGCTTTCATAGTTCAGTGTTATAATATTCAGTAGGGACCCTCAACAAATAT
ATAAAAATATGTTGCTCACTCTATAATCCTCCTATGGCTAACCTCTAGGATAGTTCTGCCACTATATTTTACTTCTTTGCCATCAGCAAGAGTAGGATTT
CATCAAGGCAAGGTAGGAATCTAAATGAAATTGATATATAAATGAATTGATCTAAATGTAAAAGCAAATGAAAAATGCATGTGTTTTTTCCTGTCAAACA
TGTATACCCTTATGTATAGAGACCAGTAGTCACGTATGGTGACTGAAACAGGATTATGTAATCCCTAAAAAGCAGAATATGTAAAAATCACATGTATGCG
TTTGGTTTAGGAATGTGCTTTTGTACTTCCACTTGAATAAAGGTGTGTTTGGTATTCTGAGAA
  • refseq_ZNF655.F1 (forward primer)
  • refseq_ZNF655.R1 (reverse primer)
  • Multiple exon skipping

Frame 0

Q*VSMEGSGTGKRRGKAAKTSLRIMDARAQLLL
RVPHPGPSLTSGALTHIRDPHPGLSPTSGTLMPG
RRRGGPHSGPCTPSPEVPPRSAGLGAVYSGPGE
AVVAPSASV*VTGQRPGPPLPS*WSLSSRAVME
EIPAQEAAGSPRVQFQSLETQSECLSPEPQFVQD
TDMEQGLTGG*LTMQQVLEFHLSPVQQNYILMT
SILILVDQI*SED*SLGFPISKPDGISQLEQDL
QVFDLETKTREVLRDDCSDGETREENKLLIPKQK
ISEEVHSYKVRVGRLKHDITQVPETREVYKSED
RLERLQEILRKFLYLEREFRQITISKETFTSEK
NNECHEPEKSFSLDSTIDADQRVLRIQNTDDNDK
YDMSFNQNSASGKHEHLNLTEDFQSSECKESLM
DLSHLNKWESIPNTEKSYKCDVCGKIFHQSSAL
TRHQRIHTREKPYKCKECEKSFSQSSSLSRHKRI
HTREKPYKCEASDKSCEASDKSCSPSSGIIQHK
KIHTRAKSYKCSSCERVFSRSVHLTQHQKIHKE
MPCKCTVCGSDFCHTSYLLEHQRVHHEEKAYEYD
EYGLAYIKQQGIHFREKPYTCSECGKDFRLNSH
LIQHQRIHTGEKAHECNECGKAFSQTSCLIQHH
KMHRKEKSYECNEYEGSFSHSSDLILQQEVLTRQ
KAFDCDVWEKNSSQRAHLVQHQSIHTKENS*M*
*RWEDIYQIQASFSI*EFTPGRNHVCTACGKAF
SHSSAIAQHQIIHTREKPSECDE*RKGISVKLLI
DSCKSIPVRNLTNVLNVANFSCY*YFHTLVTFG
EFTWE*NSIAAMNVKKPSVKETTLFSIKFTPCK
KIINVISMYVCEEIQS*PNAHSTSKNLYLRELIW
V**MADLSIGV*LVFVISEYP**TRI*CNANGK
TRHHISGFTQHRNNGEKIVDYLFMKLLIHSPNL
FHCTKI*G*LGKCSDIFSWSSFI*YVF*VGTFIF
TFLL*F*Y*KEQRWGLALLPRLVLNSWPQAILP
PVLPKCWGYRRVSLCWAYFIYRTHLSCFYFNMP
YKHFYIF*NWFLVFTTSIRYC*CTSIKTHRRK*L
I*LFLLFLAQVLSKTNS*ACSLLGILCRIALQP
QHLDSC*LTSPFS*APLEDLEVS*F*VFHQQVF
HTYLMLLVWCLIS*SEASFFKKEFD*QYIQSNIS
MKDSLS*DCSRQPNIFK*CPRF*LSCVHPQSAK
KT**GHQGANKTPNSLTTFIQCLFCMPKLRVLL
YTSTASKMLRSALGRVNIKKIHRPGMVAHTCNPS
TLGG*GGWITRSGSRDHPGQHGETPSLLKIQKL
AGCGGTRL*SQLLRRLRQENCLNLGGGGCSEPR
LHHCTPAWATEQDSLSKKHKKTKTKNHTHTHTHT
NQHHKGM*PSNRDDAVRMLISETVFQESGRSVP
GKILTIRIADKCLNSAPLSSIQDSI*SVRALCI
VVYPLPHFCILIVYRLYKRTWLFD**K*LQMLAQ
VQGHHHIPNKSS*FFR*CQNILYFSILSFITFC
E*DKCYLKFLLSVQQLRLS*FSVIIFSRDPQQI
YKNMLLTL*SSYG*PLG*FCHYILLLCHQQE*DF
IKAR*ESK*N*YINELI*M*KQMKNACVFSCQT
CIPLCIETSSHVW*LKQDYVIPKKQNM*KSHVC
VWFRNVLLYFHLNKGVFGILR

Frame 1

SELAWRAVGPEKDVEKLRKRAFESWTRGPSSSS
EFLIRGRHSHPGPSLTSGTLIRGSHPHPGPSCL
GGGGGALIRDPALRRRKCHREAPASGLSTAARER
LWWPRARVCR*QDSGQARPSPRDGHCPPEQ*WR
KYQPRKQQGHQGSSFSLWRPSLSVCPQSLSLCR
TPTWNRDSLGVS*PCSRCLSFTFLQSSRITF**P
PF*YL*TRSNPKINL*DFQFPSLMGSPSWNRIY
RSLIWKLRLEKS*EMTAQMERPEKRTSC*FLSR
KFRKKCIHTK*E*EDSNTILPKFLRLEKCISLRT
D*KDFRKF*GNFCTWRESLGK*QSARKPSPVRR
TMNVMNPKKASVWTLLLMQIREFLEYRIPMTMI
SMT*ASTRIQPLVNMNT*I*QRIFRVVNVRKA*W
ISPTLINGRASLTLRNPINVMYVGKFSIRAQPL
LDIRESILERSPTNVKNVKSLSVRAQVLVDIKE
YTLEKNLTNVKHLINPVKRLINPVVQAQA*FSIR
KFTPEPNLTNVAVVKESSVVVSTLLNIRKFTKR
CPVSVLYVAVTSAILHTYLNIRGSIMKRKPMSM
MNMGWPILNNKEFISEKSPIRVVNVEKTSD*IHI
LFSIKEFTQERKHMNVMNVEKLSVKPHALFSIT
KCIGKRNRMNVMSMRAVSVIAQILSCNKKSSPD
RKPLIVMYGKRTPVREHI*FNIRAFIPKRTHECN
EDGKIFIKFRLHSASESSHQGEIMYVLHVVKPS
VIAQPLLSIR*FTPERNPLNVTNEEKVLVLNS*
STPANLYQ*EILQMY*MWQIFHAISIFIP*SHLE
NSHGNKIPLLQ*M*KSHQSKKLPCLVSNSRHAK
RL*M**ACMCVRRFSHNPTLIQHQRIYT*ENLF
GCSKWQIFQ*EFN*SLSYQNIHSRQEFDVTQMEK
LDTTFQALPNIEIMERKLLIICL*NC*YIVPIF
FIAQKSRVDLVNAVTFSHGVPLFNMYSK*VRLF
LLFYYNFDIKKNRDGVLLCCPGWS*TPGLKRSSH
LSSQSAGVTGVCHCAGPILFIELI*AVFILICP
INIFIFFEIGS*CSQLP*DTANAPVLKHIDVSS
SFSFFCCSWPKFFPKPTLRPALY*GSYVVLLYSH
NTWIPVD*LLHSLKHL*KI*KFPSFKCFTSKYS
IPT*CCWSGVLFPKVKHLFLKKNLIDNISSPI*
V*RILSPEIVAGSQTFSNDAQGFSCLVCIHSLRR
RHDKDIREPTRLLIASLHSSSAYSACLSLEFFY
IPLRPAKCSGLLLVG*T*RRYTGRAWWLTPVIP
ALWEAEADGSRGQAVETILANMVKPRLY*KYKS*
PGVVARACNPSYSGG*GRRTA*TWEAEVAVSRD
CTTALQPGRQSRTLSQKNTKKQKQKTIHTHTHT
QISIIRECSLPTEMMLFVC*SQRQYFKRVAGLFL
VKF*PLGLQINV*ILLLSHQSRTVFEV*GLCV*
LFIHYHIFVF**STGYIKEHGFLTDKSDYRCWL
KFRATIIYLTRVHDSLGNVKTFCIFPS*AL*HFV
SKTNVI*NSCCQSSN*GFHSSVL*YSVGTLNKY
IKICCSLYNPPMANL*DSSATIFYFFAISKSRI
SSRQGRNLNEIDI*MN*SKCKSK*KMHVFFPVKH
VYPYV*RPVVTYGD*NRIM*SLKSRICKNHMYA
FGLGMCFCTST*IKVCLVF*E

Frame 2

VS*HGGQWDRKKTWKSCENEPSNHGRAGPAPPP
SSSSGAVTHIRGPHSHPGPSSGALTHIRDPHAW
AEEGGPSFGTLHSVAGSATEKRRPRGCLQRPGR
GCGGPERECVGDRTAARPAPPLVMVIVLQSSDGG
NTSPGSSRVTKGPVSVFGDPV*VSVPRASVCAG
HRHGTGTHWGLANHAAGA*VSPFSSPAELHFDD
LHFDTCRPDLIRRLIFRISNFQA*WDLPAGTGST
GL*SGN*D*RSLKR*LLRWRDQRREQAVDS*AE
NFGRSAFIQSESRKTQTRYYPSS*D*RSV*V*G
QIRKTSGNSKEISVPGERV*ANNNQQGNLHQ*EE
Q*MS*TRKKLQSGLYY*CRSESS*NTEYR*Q**
V*HELQPEFSLW*T*TLKSNRGFSE**M*GKLN
GSLPP**MGEHP*H*EIL*M*CMWENFPSELSPY
*TSENPY*REALQM*RM*KVFQSELKS*ST*KN
TH*RKTLQM*SI**IL*SV**IL*SKLRHNSA*
ENSHQSQILQM*QL*KSLQS*CPPYSTSENSQRD
AL*VYCMWQ*LLPYFIPT*TSEGPS*RESL*V*
*IWVGLY*TTRNSFQRKALYV**MWKRLQIEFT
SYSASKNSHRREST*M**MWKSFQSNLMPYSASQ
NA*EREIV*M**V*GQFQS*LRSYPATRSPHQT
ESL*L*CMGKELQSESTSSSTSEHSYQRELMNV
MKMGRYLSNSGFIQHLRVHTREKSCMYCMW*SLQ
S*LSHCSASDNSHQRETL*M*RMKKRY*C*TLN
RLLQIYTSEKSYKCIECGKFFMLLVFSYLSHIW
RIHMGIKFHCCNECEKAISQRNYLV*YQIHAMQK
DYKCNKHVCV*GDSVITQRSFNIKEFIPKRTYL
GVVNGRSFNRSLTSLCHIRISIVDKNLM*RKWK
NSTPHFRLYPTSK*WRENC*LFVYEIVNT*SQSF
SLHKNLGLTW*MQ*HFLMEFLYLICILSRYVYF
YFFIIILILKRTEMGSCFVAQAGLELLASSDPP
TCPPKVLGLQACVTVLGLFYL*NSFKLFLF*YAL
*TFLYFLKLVLSVHNFHKILLMHQY*NTST*VA
HLAFSAVLGPSSFQNQLLGLLFTRDLMSYCFTA
TTLGFLLINFSILLSTFRRFRSFLVLSVSPASIP
YLLDVAGLVSYFLK*SIFF*KRI*LTIYPVQYK
YEGFSLLRL*QAAKHFQMMPKVLAVLCASTVCE
EDMIRTSGSQQDS**PHYIHPVPILHA*A*SSFI
YLYGQQNAQVCSW*GKHKEDTQAGHGGSHL*SQ
HFGRLRRMDHEVRQSRPSWPTW*NPVSTKNTKV
SRVWWHAPVIPATQEAEAGELLEPGRRRLQ*AEI
APLHSSLGDRAGLSLKKTQKNKNKKPYTHTHTH
KSAS*GNVAFQQR*CCSYVNLRDSISREWQVCS
W*NFNH*DCR*MFEFCSSLINPGQYLKCEGFVYS
CLSITTFLYFNSLQAI*KNMAF*LIKVITDVGS
SSGPPSYT*QEFMIL*VMSKHFVFFHLKLYNIL
*VRQMLFKILVVSPAIEAFIVQCYNIQ*GPSTNI
*KYVAHSIILLWLTSRIVLPLYFTSLPSARVGF
HQGKVGI*MKLIYK*IDLNVKANEKCMCFFLSN
MYTLMYRDQ*SRMVTETGLCNP*KAEYVKITCMR
LV*ECAFVLPLE*RCVWYSE

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