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Sequences

Sequence NM_181829

GGAGTGAGGACGGTGACAGCCACGCGCGCGCGTACGCGCCCGATGCAGCGCGGCCCCGTGACCCTAGTCGGCCGCTGAGAGGCGCGCGGAGTCTGGGCCG CTGCCGTCTAGGGGTCCCGTCCCGAGGCGTCCCCGGCATCTCCGGCCCGAATCCCGGAGTGCCGGGTCGCGCCTGCACCGAAGGTCCCGGCTCCTGTGCC CTCCCTGCAGCCGTCAGGGCCCGTCCCCCAACTCCCCTTTCCGCTCAGGCAGGGTCCTCGCGGCCCATGCTGGCCGCTGGGGACCCGCGCAGCCCAGACC GTTCCCGGGCCGGGCAGCCGGCCACCATGGTGGCCCTGAGGCCTGTGCAGCAACTCCAGGGGGGCTAAAGGGCTCAGAGTGCAGGCCGTGGGGCGCGAGG GTCCCGGGCCTGAGCCCCGCGCCATGGCCGGGGCCATCGCTTCCCGCATGAGCTTCAGCTCTCTCAAGAGGAAGCAACCCAAGACGTTCACCGTGAGGAT CGTCACCATGGACGCCGAGATGGAGTTCAATTGCGAGATGAAGTGGAAAGGGAAGGACCTCTTTGATTTGGTGTGCCGGACTCTGGGGCTCCGAGAAACC TGGTTCTTTGGACTGCAGTACACAATCAAGGACACAGTGGCCTGGCTCAAAATGGACAAGAAGGTAAAGAAGCAGATTTTAGATGAAAAGATCTACTGCC CTCCTGAGGCTTCTGTGCTCCTGGCTTCTTACGCCGTCCAGGCCAAGTATGGTGACTACGACCCCAGTGTTCACAAGCGGGGATTTTTGGCCCAAGAGGA ATTGCTTCCAAAAAGGGTAATAAATCTGTATCAGATGACTCCGGAAATGTGGGAGGAGAGAATTACTGCTTGGTACGCAGAGCACCGAGGCCGAGCCAGG GATGAAGCTGAAATGGAATATCTGAAGATAGCTCAGGACCTGGAGATGTACGGTGTGAACTACTTTGCAATCCGGAATAAAAAGGGCACAGAGCTGCTGC TTGGAGTGGATGCCCTGGGGCTTCACATTTATGACCCTGAGAACAGACTGACCCCCAAGATCTCCTTCCCGTGGAATGAAATCCGAAACATCTCGTACAG TGACAAGGAGTTTACTATTAAACCACTGGATAAGAAAATTGATGTCTTCAAGTTTAACTCCTCAAAGCTTCGTGTTAATAAGCTGATTCTCCAGCTATGT ATCGGGAACCATGATCTATTTATGAGGAGAAGGAAAGCCGATTCTTTGGAAGTTCAGCAGATGAAAGCCCAGGCCAGGGAGGAGAAGGCTAGAAAGCAGA TGGAGCGGCAGCGCCTCGCTCGAGAGAAGCAGATGAGGGAGGAGGCTGAACGCACGAGGGATGAGTTGGAGAGGAGGCTGCTGCAGATGAAAGAAGAAGC AACAATGGCCAACGAAGCACTGATGCGGTCTGAGGAGACAGCTGACCTGTTGGCTGAAAAGGCCCAGATCACCGAGGAGGAGGCAAAACTTCTGGCCCAG AAGGCCGCAGAGGCTGAGCAGGAAATGCAGCGCATCAAGGCCACAGCGATTCGCACGGAGGAGGAGAAGCGCCTGATGGAGCAGAAGGTGCTGGAAGCCG AGGTGCTGGCACTGAAGATGGCTGAGGAGTCAGAGAGGAGGGCCAAAGAGGCAGATCAGCTGAAGCAGGACCTGCAGGAAGCACGCGAGGCGGAGCGAAG AGCCAAGCAGAAGCTCCTGGAGATTGCCACCAAGCCCACGTACCCGCCCATGAACCCAATTCCAGCACCGTTGCCTCCTGACATACCAAGCTTCAACCTC ATTGGTGACAGCCTGTCTTTCGACTTCAAAGATACTGACATGAAGCGGCTTTCCATGGAGATAGAGAAAGAAAAAGTGGAATACATGGAAAAGAGCAAGC ATCTGCAGGAGCAGCTCAATGAACTCAAGACAGAAATCGAGGCCTTGAAACTGAAAGAGAGGGAGACAGCTCTGGATATTCTGCACAATGAGAACTCCGA CAGGGGTGGCAGCAGCAAGCACAATACCATTAAAAAGCCTCAAGCCCAAGGCAGAAGACCTATCTGCATTTGAGCCCTCAAACTCACCTTGCAGAGCGCC AAGTCCCGAGTGGCCTTCTTTGAAGAGCTCTAGCAGGTGACCCAGCCACCCCAGGACCTGCCACTTCTCCTGCTACCGGGACCGCGGGATGGACCAGATA TCAAGAGAGCCATCCATAGGGAGCTGGCTGGGGGTTTCCGTGGGAGCTCCAGAACTTTCCCCAGCTGAGTGAAGAGCCCAGCCCCTCTTATGTGCAATTG CCTTGAACTACGACCCTGTAGAGATTTCTCTCATGGCGTTCTAGTTCTCTGACCTGAGTCTTTGTTTTAAGAAGTATTTGTCTTCCTTTGTCTAATGTGG GATTCCTGACTCCCTTCGTCCAAGGCACCGGTGTGTGTGTGTCTTGCACTCCAGAGCTGACCTCCACCGCCCAGCCTGGGAAGTCATTGTAGGGAGTGAG ACACTGAAGCCCTGAGAAGCCAGTGCCATCATCCCCACCCCGCCCAGGGTTCCGGAACATTCATTCCCCCACCGGTGAGGACCTGGCATGCAGCGAAGCA GCCCAGCCCGGCGGATCCCAGGCCAGCACGCCTGCCGGCTTCTCATCGTCAGGGAGCCCGCCCAGAGCTCGTGACGAGCAAGTGCTGGGTCCCCGCCAGG CACCCCGAGGCGGCGCTCTGGCTGGCAGCTGGTGGGGAATAGGCAGGGCAGCTGTGGCTGGGGAGAGACTTTAGGCAGAAGCTGTGATGCAGGCTGACTG CCAGCCGAGGGGCTGGGTAGTGCCGTGCGGGAGCTGATGGTACAGGGCACTCGCTGTCCCCCTCCGGCCACCCTAGACCAGGGTCCGAGAGGCAGGCAGG AGCCACTCATGTCTTCCCCATTGCCCGACGCCCATAGACGCTCCTTCCTGTGTGGGGCTGGGGTACTCCCTGGTCGTACTGCAGTCAGCACCCGTAACCC GGCTATGACCAGGGATCTGTAAGCCCTGTGGCTCCACAGGTGCTGCTTCTCACTGGCCCAGACTCTGAGCTCCACCGGCCCAGTCTGCACGGCCCATCTG CTTCACCTTCCCTCCCAGCCACGTGCCAGTGGCCACAGCCCACTTCCCAACCCACTGTTGTACCCAGGCCTCACTTTGCTGTTGCCCTTGTCCCTCTTCG GGCCCTGAATTTTCTGTTCCCTGGGGGCCAGCCAGGGCCCTTTGTGCCCCTCCCAGCACAGGCCTGATGCAGGTGTCCACTCACAGGTGGCGCTCACCTA GGCTGTCACAGGACCCACCTCCATGCCAGGCAACAGAGGGCCACAGAACCACCCCCACGGCTCACTCCTTGGTCTGGGGCCACCTTCTTGCCCTTTCTTT TTTTTTTTTTTTTTTTTTTTCCGAGATGGAGTCTCTCTCTGTCACCCAGGTGGGAGTGTGGTGGCACAATCTCGGCTCACTGCAACCTCCACCTCCTGAG TTCAAGCAATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGCATGCACCACCACACTTGGCCAATGTTCTGTATTTTTAATAGGGACGGGGTT TTGCCATGTTAGCTAGGCTGGTCTCAAACTCACACCTGGGATTACAGGCATGAGCCACTGCACCCAGCCCCTTCTTGCCCTTTCTTTTCTCCATGGCTGA TGCTGCTGTGGCCAGCCAGGGCCCTTGAGATCCTTCCAGTTTGGCTGTTATGCAAAGCAGGTGATTTGTCTTAATCAGATAAAAGATAGAGGCTATGGGG GCCTCAAGATTTTTGGAGAGCAGAGGTGGTCTCTGGCAATTCCATCTGGTTTTGAGAAACTTAGCAGCTCACAGAGCACAGAGATCCTGCCTTCTTCCTA CTATCAGGCTGACCTAATGGGGTTGGGCTGCTCGGCAACTGCTTGGGTCACCTTGCCCCAAGGAAACCAGCCCTGGGTGCCACCCAGCCACTTAGGGTCT ACAGGGTGGGACTCCAGACCTAGAGCGTAAGTATGGATGTTGTGGCCCTGTGTCTTCCTAGTGTGACCCAGCCAGGAGCGGAAGCTTCAGGCGTTTGTAA AGTGAGGTCTGGCTCTGCCTCCTCCGTTTTTTTTTTTTTTCTGTTTCTGTTTCTGTTTTTTTTTTGAGATGGAGTCTCGCTCTGTCGCCCAGGCTGGAGT GCAGTGTCACGATCTCAGCTCACTGCAACCTCCGCCTCCCAGGTACAAGAGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGTGTGCCA CCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCGCCATGTTAGCCAGACTGGTCTCGAACTCCTGACCTCAGGTGATCCTCCCACCCCG GCTTCCCAAAGTTCTGGGATTATAGGCGTGAGCCACCATGCCCGGTCTCTTCTCAGTCTTGAAGCCCATCCCTGGATTTCCACCAGGAGTTACTTTCCTC CTGACCTGTAAATTTGTTCTTTAACAATGGCTGCAGGTGGGAGCATATGGTGGTTTATAAAAACGCTGTCGGGCTTTGTTTCCTTCTTTAGCTGCCGTGT CTACTTCTGAAGTCTGGGAAGTGCCAAGCCACGCGGCCTCAAGGGAGCTGGCTGGTGTTTGAGCTGTGGCAGAAGCACCTGGGGCTCCAGGGAGCAGGCT GGGAACTGCAGGACCTTGCTCAGCCAGGAGCACTTCCCCCTCCTTGAGGCAGGAATACTGAGGTGCCTCCCCACAGATGGAGAAGGTGGAGAGGAGGATG GGCCTCAGGAGCATCTCAAGCCCCAGTAGCAGGAGAAAGAAAGAAAGAGATGCCTGGTTTTCACAGACTGGTTCCTGTGGCTGGGATGACTGCATCCTTT TTTTTTTTTTTTTGAGACGGAGTTTTTGCCTTTGTCGCCCAGGCTGGAGTGCAATGGCGTGATCTCGGCTCACCGCAACCTCCGCCTCCCGGATTCAAGC AATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCACGCACCTCCACGTCCGGCTAATTTTGTATTTTTAGTGGAGACGGGGTTTCTCCATGT CGGTCAGGCTGGTCTCGAACTCCCGACCTCAGGTGATCTGCCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCTTGGCCAGAC TGCGTCCTTTTTAAGCGAACATTTTAGGGCCTGGGAGTTTGTCAAGTAAGGAAGTCTCAAGCCCAAAGAGCAGCGTCCTGACCATGGTGGTTTCATTACG AGCCCTTCTGCTGGCTCTCAGGCAGAAGCCCCACAGCACCGGGACCATTCATGAGGTCACTGCCCAGCTCATGATGTCCGTGAGGCTGTCCTTTTGGCCA GTAGCCGTGTGCAGCTGTGTGGCACAGATGGCTTCGTTCATCCTGATCAAGGCCCCACCTCAGCCACAGCAGTCCCCCCAACCTGTGTTGTCCACCCTAT TATTCATGTACCTGCCAGGCCCTGCTAGATAGCACCCCGTGGCATTACATAACACTTCATGAGTGGCTGTGTCTTGTAATTTTGGGGACAGGTTTCTCTC TTTCCCTCTCTTTTTTTTGTCAAAAGCCCAGAGACTGACAACCAGCTGCAGTGTCTAAGTGTTCCTCACTGACAGGGTGGGGCCTCACCACCCCTGGAGG GAGCAGCGTTGGCAGGGAGACAGCCTGGCCCAGTGACCCTGGGCCCAAGCCAGCCCCTCCAGGGCTTTCAGGGAAGCGCCATCCATTTTCAAAGATGTCA AACGTCACTTCTTCCTGTAGGGCCCGAGTCCTGCCTCCTATCAGGGCCAGATCATAGAAGGCTATTTTCTATTCTGGGGAACGATTATAACTTAAATGAT TGTTTTAATAAAAATTCTAAGCTGGAAAA

refseq_NF2.F2 (forward primer)
refseq_NF2.R2 (reverse primer)
Single exon skipping

Frame 0

GVRTVTATRARTRPMQRGPVTLVGR*EARGVWA AAV*GSRPEASPASPARIPECRVAPAPKVPAPVP SLQPSGPVPQLPFPLRQGPRGPCWPLGTRAAQT VPGPGSRPPWWP*GLCSNSRGAKGLRVQAVGRE GPGPEPRAMAGAIASRMSFSSLKRKQPKTFTVRI VTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRET WFFGLQYTIKDTVAWLKMDKKVKKQILDEKIYC PPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEE LLPKRVINLYQMTPEMWEERITAWYAEHRGRAR DEAEMEYLKIAQDLEMYGVNYFAIRNKKGTELL LGVDALGLHIYDPENRLTPKISFPWNEIRNISYS DKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLC IGNHDLFMRRRKADSLEVQQMKAQAREEKARKQ MERQRLAREKQMREEAERTRDELERRLLQMKEEA TMANEALMRSEETADLLAEKAQITEEEAKLLAQ KAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEA EVLALKMAEESERRAKEADQLKQDLQEAREAERR AKQKLLEIATKPTYPPMNPIPAPLPPDIPSFNL IGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSK HLQEQLNELKTEIEALKLKERETALDILHNENSD RGGSSKHNTIKKPQAQGRRPICI*ALKLTLQSA KSRVAFFEEL*QVTQPPQDLPLLLLPGPRDGPD IKRAIHRELAGGFRGSSRTFPS*VKSPAPLMCNC LELRPCRDFSHGVLVL*PESLF*EVFVFLCLMW DS*LPSSKAPVCVCLALQS*PPPPSLGSHCRE* DTEALRSQCHHPHPAQGSGTFIPPPVRTWHAAKQ PSPADPRPARLPASHRQGARPELVTSKCWVPAR HPEAALWLAAGGE*AGQLWLGRDFRQKL*CRLT ASRGAG*CRAGADGTGHSLSPSGHPRPGSERQAG ATHVFPIARRP*TLLPVWGWGTPWSYCSQHP*P GYDQGSVSPVAPQVLLLTGPDSELHRPSLHGPS ASPSLPATCQWPQPTSQPTVVPRPHFAVALVPLR ALNFLFPGGQPGPFVPLPAQA*CRCPLTGGAHL GCHRTHLHARQQRATEPPPRLTPWSGATFLPFL FFFFFFFPRWSLSLSPRWECGGTISAHCNLHLLS SSNSPASASQVAGTTGMHHHTWPMFCIFNRDGV LPC*LGWSQTHTWDYRHEPLHPAPSCPFFSPWL MLLWPARALEILPVWLLCKAGDLS*SDKR*RLWG PQDFWRAEVVSGNSIWF*ET*QLTEHRDPAFFL LSG*PNGVGLLGNCLGHLAPRKPALGATQPLRV YRVGLQT*SVSMDVVALCLPSVTQPGAEASGVCK VRSGSASSVFFFFLFLFLFFF*DGVSLCRPGWS AVSRSQLTATSASQVQEILLPQPPE*LGLQACA TMPG*FLYF**RWGFAMLARLVSNS*PQVILPPR LPKVLGL*A*ATMPGLFSVLKPIPGFPPGVTFL LTCKFVL*QWLQVGAYGGL*KRCRALFPSLAAV STSEVWEVPSHAASRELAGV*AVAEAPGAPGSRL GTAGPCSARSTSPSLRQEY*GASPQMEKVERRM GLRSISSPSSRRKKERDAWFSQTGSCGWDDCIL FFFFLRRSFCLCRPGWSAMA*SRLTATSASRIQA ILLPQPPE*LGLQARTSTSG*FCIFSGDGVSPC RSGWSRTPDLR*SAHLGLPKCWDYRHEPPRLAR LRPF*ANILGPGSLSSKEVSSPKSSVLTMVVSLR ALLLALRQKPHSTGTIHEVTAQLMMSVRLSFWP VAVCSCVAQMASFILIKAPPQPQQSPQPVLSTL LFMYLPGPAR*HPVALHNTS*VAVSCNFGDRFLS FPLFFLSKAQRLTTSCSV*VFLTDRVGPHHPWR EQRWQGDSLAQ*PWAQASPSRAFREAPSIFKDV KRHFFL*GPSPASYQGQIIEGYFLFWGTIIT*MI VLIKILSWK

Frame 1

E*GR*QPRARVRARCSAAP*P*SAAERRAESGP LPSRGPVPRRPRHLRPESRSAGSRLHRRSRLLC PPCSRQGPSPNSPFRSGRVLAAHAGRWGPAQPRP FPGRAAGHHGGPEACAATPGGLKGSECRPWGAR VPGLSPAPWPGPSLPA*ASALSRGSNPRRSP*G SSPWTPRWSSIAR*SGKGRTSLIWCAGLWGSEKP GSLDCSTQSRTQWPGSKWTRR*RSRF*MKRSTA LLRLLCSWLLTPSRPSMVTTTPVFTSGDFWPKR NCFQKG**ICIR*LRKCGRRELLLGTQSTEAEPG MKLKWNI*R*LRTWRCTV*TTLQSGIKRAQSCC LEWMPWGFTFMTLRTD*PPRSPSRGMKSETSRT VTRSLLLNHWIRKLMSSSLTPQSFVLIS*FSSYV SGTMIYL*GEGKPILWKFSR*KPRPGRRRLESR WSGSASLERSR*GRRLNARGMSWRGGCCR*KKK QQWPTKH*CGLRRQLTCWLKRPRSPRRRQNFWPR RPQRLSRKCSASRPQRFARRRRSA*WSRRCWKP RCWH*RWLRSQRGGPKRQIS*SRTCRKHARRSE EPSRSSWRLPPSPRTRP*TQFQHRCLLTYQASTS LVTACLSTSKILT*SGFPWR*RKKKWNTWKRAS ICRSSSMNSRQKSRP*N*KRGRQLWIFCTMRTP TGVAAASTIPLKSLKPKAEDLSAFEPSNSPCRAP SPEWPSLKSSSR*PSHPRTCHFSCYRDRGMDQI SREPSIGSWLGVSVGAPELSPAE*RAQPLLCAI ALNYDPVEISLMAF*FSDLSLCFKKYLSSFV*CG IPDSLRPRHRCVCVLHSRADLHRPAWEVIVGSE TLKP*EASAIIPTPPRVPEHSFPHR*GPGMQRS SPARRIPGQHACRLLIVREPAQSS*RASAGSPPG TPRRRSGWQLVGNRQGSCGWGETLGRSCDAG*L PAEGLGSAVRELMVQGTRCPPPATLDQGPRGRQ EPLMSSPLPDAHRRSFLCGAGVLPGRTAVSTRNP AMTRDL*ALWLHRCCFSLAQTLSSTGPVCTAHL LHLPSQPRASGHSPLPNPLLYPGLTLLLPLSLF GP*IFCSLGASQGPLCPSQHRPDAGVHSQVALT* AVTGPTSMPGNRGPQNHPHGSLLGLGPPSCPFF FFFFFFFRDGVSLCHPGGSVVAQSRLTATSTS* VQAILLPQPPK*LGLQACTTTLGQCSVFLIGTGF CHVS*AGLKLTPGITGMSHCTQPLLALSFLHG* CCCGQPGPLRSFQFGCYAKQVICLNQIKDRGYG GLKIFGEQRWSLAIPSGFEKLSSSQSTEILPSSY YQADLMGLGCSATAWVTLPQGNQPWVPPSHLGS TGWDSRPRA*VWMLWPCVFLV*PSQERKLQAFV K*GLALPPPFFFFFCFCFCFFFEMESRSVAQAGV QCHDLSSLQPPPPRYKRFSCLSLPSSWDYRRVP PCLANFCIFSRDGVSPC*PDWSRTPDLR*SSHP GFPKFWDYRREPPCPVSSQS*SPSLDFHQELLSS *PVNLFFNNGCRWEHMVVYKNAVGLCFLL*LPC LLLKSGKCQATRPQGSWLVFELWQKHLGLQGAG WELQDLAQPGALPPP*GRNTEVPPHRWRRWRGGW ASGASQAPVAGERKKEMPGFHRLVPVAGMTASF FFFF*DGVFAFVAQAGVQWRDLGSPQPPPPGFK QFSCLSLPSSWDYRHAPPRPANFVFLVETGFLHV GQAGLELPTSGDLPTSASQSAGITGMSHRAWPD CVLFKRTF*GLGVCQVRKSQAQRAAS*PWWFHY EPFCWLSGRSPTAPGPFMRSLPSS*CP*GCPFGQ *PCAAVWHRWLRSS*SRPHLSHSSPPNLCCPPY YSCTCQALLDSTPWHYITLHEWLCLVILGTGFS LSLSFFCQKPRD*QPAAVSKCSSLTGWGLTTPGG SSVGRETAWPSDPGPKPAPPGLSGKRHPFSKMS NVTSSCRARVLPPIRARS*KAIFYSGERL*LK* LF**KF*AGK

Frame 2

SEDGDSHARAYAPDAARPRDPSRPLRGARSLGR CRLGVPSRGVPGISGPNPGVPGRACTEGPGSCA LPAAVRARPPTPLSAQAGSSRPMLAAGDPRSPD RSRAGQPATMVALRPVQQLQGG*RAQSAGRGARG SRA*APRHGRGHRFPHELQLSQEEATQDVHRED RHHGRRDGVQLRDEVEREGPL*FGVPDSGAPRN LVLWTAVHNQGHSGLAQNGQEGKEADFR*KDLLP S*GFCAPGFLRRPGQVW*LRPQCSQAGIFGPRG IASKKGNKSVSDDSGNVGGENYCLVRRAPRPSQ G*S*NGISEDSSGPGDVRCELLCNPE*KGHRAAA WSGCPGASHL*P*EQTDPQDLLPVE*NPKHLVQ *QGVYY*TTG*EN*CLQV*LLKASC**ADSPAM YREP*SIYEEKESRFFGSSADESPGQGGEG*KAD GAAAPRSREADEGGG*THEG*VGEEAAADERRS NNGQRSTDAV*GDS*PVG*KGPDHRGGGKTSGP EGRRG*AGNAAHQGHSDSHGGGEAPDGAEGAGSR GAGTEDG*GVREEGQRGRSAEAGPAGSTRGGAK SQAEAPGDCHQAHVPAHEPNSSTVAS*HTKLQP HW*QPVFRLQRY*HEAAFHGDRERKSGIHGKEQA SAGAAQ*TQDRNRGLETEREGDSSGYSAQ*ELR QGWQQQAQYH*KASSPRQKTYLHLSPQTHLAER QVPSGLL*RALAGDPATPGPATSPATGTAGWTRY QESHP*GAGWGFPWELQNFPQLSEEPSPSYVQL P*TTTL*RFLSWRSSSLT*VFVLRSICLPLSNV GFLTPFVQGTGVCVSCTPELTSTAQPGKSL*GVR H*SPEKPVPSSPPRPGFRNIHSPTGEDLACSEA AQPGGSQASTPAGFSSSGSPPRARDEQVLGPRQ APRGGALAGSWWGIGRAAVAGERL*AEAVMQADC QPRGWVVPCGS*WYRALAVPLRPP*TRVREAGR SHSCLPHCPTPIDAPSCVGLGYSLVVLQSAPVT RL*PGICKPCGSTGAASHWPRL*APPAQSARPIC FTFPPSHVPVATAHFPTHCCTQASLCCCPCPSS GPEFSVPWGPARALCAPPSTGLMQVSTHRWRSP RLSQDPPPCQATEGHRTTPTAHSLVWGHLLALSF FFFFFFSEMESLSVTQVGVWWHNLGSLQPPPPE FKQFSCLSLPSSWDYRHAPPHLANVLYF**GRG FAMLARLVSNSHLGLQA*ATAPSPFLPFLFSMAD AAVASQGP*DPSSLAVMQSR*FVLIR*KIEAMG ASRFLESRGGLWQFHLVLRNLAAHRAQRSCLLP TIRLT*WGWAARQLLGSPCPKETSPGCHPAT*GL QGGTPDLERKYGCCGPVSS*CDPARSGSFRRL* SEVWLCLLRFFFFSVSVSVFFLRWSLALSPRLE CSVTISAHCNLRLPGTRDSPASASRVAGTTGVCH HAWLIFVFLVEMGFRHVSQTGLELLTSGDPPTP ASQSSGIIGVSHHARSLLSLEAHPWISTRSYFP PDL*ICSLTMAAGGSIWWFIKTLSGFVSFFSCRV YF*SLGSAKPRGLKGAGWCLSCGRSTWGSREQA GNCRTLLSQEHFPLLEAGILRCLPTDGEGGEED GPQEHLKPQ*QEKERKRCLVFTDWFLWLG*LHPF FFFFETEFLPLSPRLECNGVISAHRNLRLPDSS NSPASASRVAGITGTHLHVRLILYF*WRRGFSM SVRLVSNSRPQVICPPRPPKVLGLQA*ATALGQT ASFLSEHFRAWEFVK*GSLKPKEQRPDHGGFIT SPSAGSQAEAPQHRDHS*GHCPAHDVREAVLLA SSRVQLCGTDGFVHPDQGPTSATAVPPTCVVHPI IHVPARPC*IAPRGIT*HFMSGCVL*FWGQVSL FPSLFFVKSPETDNQLQCLSVPH*QGGASPPLE GAALAGRQPGPVTLGPSQPLQGFQGSAIHFQRCQ TSLLPVGPESCLLSGPDHRRLFSILGNDYNLND CFNKNSKLE

Sequence NM_181833

GGAGTGAGGACGGTGACAGCCACGCGCGCGCGTACGCGCCCGATGCAGCGCGGCCCCGTGACCCTAGTCGGCCGCTGAGAGGCGCGCGGAGTCTGGGCCG CTGCCGTCTAGGGGTCCCGTCCCGAGGCGTCCCCGGCATCTCCGGCCCGAATCCCGGAGTGCCGGGTCGCGCCTGCACCGAAGGTCCCGGCTCCTGTGCC CTCCCTGCAGCCGTCAGGGCCCGTCCCCCAACTCCCCTTTCCGCTCAGGCAGGGTCCTCGCGGCCCATGCTGGCCGCTGGGGACCCGCGCAGCCCAGACC GTTCCCGGGCCGGGCAGCCGGCCACCATGGTGGCCCTGAGGCCTGTGCAGCAACTCCAGGGGGGCTAAAGGGCTCAGAGTGCAGGCCGTGGGGCGCGAGG GTCCCGGGCCTGAGCCCCGCGCCATGGCCGGGGCCATCGCTTCCCGCATGAGCTTCAGCTCTCTCAAGAGGAAGCAACCCAAGACGTTCACCGTGAGGAT CGTCACCATGGACGCCGAGATGGAGTTCAATTGCGAGATGAAGTGGAAAGGGAAGGACCTCTTTGATTTGGTGTGCCGGACTCTGGGGCTCCGAGAAACC TGGTTCTTTGGACTGCAGTACACAATCAAGGACACAGTGGCCTGGCTCAAAATGGACAAGAAGGTACTGGATCATGATGTTTCAAAGGAAGAACCAGTCA CCTTTCACTTCTTGGCCAAATTTTATCCTGAGAATGCTGAAGAGGAGCTGGTTCAGGAGATCACACAACATTTATTCTTCTTACAGGTAAAGAAGCAGAT TTTAGATGAAAAGATCTACTGCCCTCCTGAGGCTTCTGTGCTCCTGGCTTCTTACGCCGTCCAGGCCAAGCTCACCTTGCAGAGCGCCAAGTCCCGAGTG GCCTTCTTTGAAGAGCTCTAGCAGGTGACCCAGCCACCCCAGGACCTGCCACTTCTCCTGCTACCGGGACCGCGGGATGGACCAGATATCAAGAGAGCCA TCCATAGGGAGCTGGCTGGGGGTTTCCGTGGGAGCTCCAGAACTTTCCCCAGCTGAGTGAAGAGCCCAGCCCCTCTTATGTGCAATTGCCTTGAACTACG ACCCTGTAGAGATTTCTCTCATGGCGTTCTAGTTCTCTGACCTGAGTCTTTGTTTTAAGAAGTATTTGTCTTCCTTTGTCTAATGTGGGATTCCTGACTC CCTTCGTCCAAGGCACCGGTGTGTGTGTGTCTTGCACTCCAGAGCTGACCTCCACCGCCCAGCCTGGGAAGTCATTGTAGGGAGTGAGACACTGAAGCCC TGAGAAGCCAGTGCCATCATCCCCACCCCGCCCAGGGTTCCGGAACATTCATTCCCCCACCGGTGAGGACCTGGCATGCAGCGAAGCAGCCCAGCCCGGC GGATCCCAGGCCAGCACGCCTGCCGGCTTCTCATCGTCAGGGAGCCCGCCCAGAGCTCGTGACGAGCAAGTGCTGGGTCCCCGCCAGGCACCCCGAGGCG GCGCTCTGGCTGGCAGCTGGTGGGGAATAGGCAGGGCAGCTGTGGCTGGGGAGAGACTTTAGGCAGAAGCTGTGATGCAGGCTGACTGCCAGCCGAGGGG CTGGGTAGTGCCGTGCGGGAGCTGATGGTACAGGGCACTCGCTGTCCCCCTCCGGCCACCCTAGACCAGGGTCCGAGAGGCAGGCAGGAGCCACTCATGT CTTCCCCATTGCCCGACGCCCATAGACGCTCCTTCCTGTGTGGGGCTGGGGTACTCCCTGGTCGTACTGCAGTCAGCACCCGTAACCCGGCTATGACCAG GGATCTGTAAGCCCTGTGGCTCCACAGGTGCTGCTTCTCACTGGCCCAGACTCTGAGCTCCACCGGCCCAGTCTGCACGGCCCATCTGCTTCACCTTCCC TCCCAGCCACGTGCCAGTGGCCACAGCCCACTTCCCAACCCACTGTTGTACCCAGGCCTCACTTTGCTGTTGCCCTTGTCCCTCTTCGGGCCCTGAATTT TCTGTTCCCTGGGGGCCAGCCAGGGCCCTTTGTGCCCCTCCCAGCACAGGCCTGATGCAGGTGTCCACTCACAGGTGGCGCTCACCTAGGCTGTCACAGG ACCCACCTCCATGCCAGGCAACAGAGGGCCACAGAACCACCCCCACGGCTCACTCCTTGGTCTGGGGCCACCTTCTTGCCCTTTCTTTTTTTTTTTTTTT TTTTTTTTCCGAGATGGAGTCTCTCTCTGTCACCCAGGTGGGAGTGTGGTGGCACAATCTCGGCTCACTGCAACCTCCACCTCCTGAGTTCAAGCAATTC TCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGCATGCACCACCACACTTGGCCAATGTTCTGTATTTTTAATAGGGACGGGGTTTTGCCATGTTAG CTAGGCTGGTCTCAAACTCACACCTGGGATTACAGGCATGAGCCACTGCACCCAGCCCCTTCTTGCCCTTTCTTTTCTCCATGGCTGATGCTGCTGTGGC CAGCCAGGGCCCTTGAGATCCTTCCAGTTTGGCTGTTATGCAAAGCAGGTGATTTGTCTTAATCAGATAAAAGATAGAGGCTATGGGGGCCTCAAGATTT TTGGAGAGCAGAGGTGGTCTCTGGCAATTCCATCTGGTTTTGAGAAACTTAGCAGCTCACAGAGCACAGAGATCCTGCCTTCTTCCTACTATCAGGCTGA CCTAATGGGGTTGGGCTGCTCGGCAACTGCTTGGGTCACCTTGCCCCAAGGAAACCAGCCCTGGGTGCCACCCAGCCACTTAGGGTCTACAGGGTGGGAC TCCAGACCTAGAGCGTAAGTATGGATGTTGTGGCCCTGTGTCTTCCTAGTGTGACCCAGCCAGGAGCGGAAGCTTCAGGCGTTTGTAAAGTGAGGTCTGG CTCTGCCTCCTCCGTTTTTTTTTTTTTTCTGTTTCTGTTTCTGTTTTTTTTTTGAGATGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGTCACGA TCTCAGCTCACTGCAACCTCCGCCTCCCAGGTACAAGAGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGTGTGCCACCATGCCTGGCT AATTTTTGTATTTTTAGTAGAGATGGGGTTTCGCCATGTTAGCCAGACTGGTCTCGAACTCCTGACCTCAGGTGATCCTCCCACCCCGGCTTCCCAAAGT TCTGGGATTATAGGCGTGAGCCACCATGCCCGGTCTCTTCTCAGTCTTGAAGCCCATCCCTGGATTTCCACCAGGAGTTACTTTCCTCCTGACCTGTAAA TTTGTTCTTTAACAATGGCTGCAGGTGGGAGCATATGGTGGTTTATAAAAACGCTGTCGGGCTTTGTTTCCTTCTTTAGCTGCCGTGTCTACTTCTGAAG TCTGGGAAGTGCCAAGCCACGCGGCCTCAAGGGAGCTGGCTGGTGTTTGAGCTGTGGCAGAAGCACCTGGGGCTCCAGGGAGCAGGCTGGGAACTGCAGG ACCTTGCTCAGCCAGGAGCACTTCCCCCTCCTTGAGGCAGGAATACTGAGGTGCCTCCCCACAGATGGAGAAGGTGGAGAGGAGGATGGGCCTCAGGAGC ATCTCAAGCCCCAGTAGCAGGAGAAAGAAAGAAAGAGATGCCTGGTTTTCACAGACTGGTTCCTGTGGCTGGGATGACTGCATCCTTTTTTTTTTTTTTT TGAGACGGAGTTTTTGCCTTTGTCGCCCAGGCTGGAGTGCAATGGCGTGATCTCGGCTCACCGCAACCTCCGCCTCCCGGATTCAAGCAATTCTCCTGCC TCAGCCTCCCGAGTAGCTGGGATTACAGGCACGCACCTCCACGTCCGGCTAATTTTGTATTTTTAGTGGAGACGGGGTTTCTCCATGTCGGTCAGGCTGG TCTCGAACTCCCGACCTCAGGTGATCTGCCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCTTGGCCAGACTGCGTCCTTTTT AAGCGAACATTTTAGGGCCTGGGAGTTTGTCAAGTAAGGAAGTCTCAAGCCCAAAGAGCAGCGTCCTGACCATGGTGGTTTCATTACGAGCCCTTCTGCT GGCTCTCAGGCAGAAGCCCCACAGCACCGGGACCATTCATGAGGTCACTGCCCAGCTCATGATGTCCGTGAGGCTGTCCTTTTGGCCAGTAGCCGTGTGC AGCTGTGTGGCACAGATGGCTTCGTTCATCCTGATCAAGGCCCCACCTCAGCCACAGCAGTCCCCCCAACCTGTGTTGTCCACCCTATTATTCATGTACC TGCCAGGCCCTGCTAGATAGCACCCCGTGGCATTACATAACACTTCATGAGTGGCTGTGTCTTGTAATTTTGGGGACAGGTTTCTCTCTTTCCCTCTCTT TTTTTTGTCAAAAGCCCAGAGACTGACAACCAGCTGCAGTGTCTAAGTGTTCCTCACTGACAGGGTGGGGCCTCACCACCCCTGGAGGGAGCAGCGTTGG CAGGGAGACAGCCTGGCCCAGTGACCCTGGGCCCAAGCCAGCCCCTCCAGGGCTTTCAGGGAAGCGCCATCCATTTTCAAAGATGTCAAACGTCACTTCT TCCTGTAGGGCCCGAGTCCTGCCTCCTATCAGGGCCAGATCATAGAAGGCTATTTTCTATTCTGGGGAACGATTATAACTTAAATGATTGTTTTAATAAA AATTCTAAGCTGGAAAA

refseq_NF2.F2 (forward primer)
refseq_NF2.R2 (reverse primer)
Single exon skipping

Frame 0

GVRTVTATRARTRPMQRGPVTLVGR*EARGVWA AAV*GSRPEASPASPARIPECRVAPAPKVPAPVP SLQPSGPVPQLPFPLRQGPRGPCWPLGTRAAQT VPGPGSRPPWWP*GLCSNSRGAKGLRVQAVGRE GPGPEPRAMAGAIASRMSFSSLKRKQPKTFTVRI VTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRET WFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPV TFHFLAKFYPENAEEELVQEITQHLFFLQVKKQI LDEKIYCPPEASVLLASYAVQAKLTLQSAKSRV AFFEEL*QVTQPPQDLPLLLLPGPRDGPDIKRA IHRELAGGFRGSSRTFPS*VKSPAPLMCNCLELR PCRDFSHGVLVL*PESLF*EVFVFLCLMWDS*L PSSKAPVCVCLALQS*PPPPSLGSHCRE*DTEA LRSQCHHPHPAQGSGTFIPPPVRTWHAAKQPSPA DPRPARLPASHRQGARPELVTSKCWVPARHPEA ALWLAAGGE*AGQLWLGRDFRQKL*CRLTASRG AG*CRAGADGTGHSLSPSGHPRPGSERQAGATHV FPIARRP*TLLPVWGWGTPWSYCSQHP*PGYDQ GSVSPVAPQVLLLTGPDSELHRPSLHGPSASPS LPATCQWPQPTSQPTVVPRPHFAVALVPLRALNF LFPGGQPGPFVPLPAQA*CRCPLTGGAHLGCHR THLHARQQRATEPPPRLTPWSGATFLPFLFFFF FFFPRWSLSLSPRWECGGTISAHCNLHLLSSSNS PASASQVAGTTGMHHHTWPMFCIFNRDGVLPC* LGWSQTHTWDYRHEPLHPAPSCPFFSPWLMLLW PARALEILPVWLLCKAGDLS*SDKR*RLWGPQDF WRAEVVSGNSIWF*ET*QLTEHRDPAFFLLSG* PNGVGLLGNCLGHLAPRKPALGATQPLRVYRVG LQT*SVSMDVVALCLPSVTQPGAEASGVCKVRSG SASSVFFFFLFLFLFFF*DGVSLCRPGWSAVSR SQLTATSASQVQEILLPQPPE*LGLQACATMPG *FLYF**RWGFAMLARLVSNS*PQVILPPRLPKV LGL*A*ATMPGLFSVLKPIPGFPPGVTFLLTCK FVL*QWLQVGAYGGL*KRCRALFPSLAAVSTSE VWEVPSHAASRELAGV*AVAEAPGAPGSRLGTAG PCSARSTSPSLRQEY*GASPQMEKVERRMGLRS ISSPSSRRKKERDAWFSQTGSCGWDDCILFFFF LRRSFCLCRPGWSAMA*SRLTATSASRIQAILLP QPPE*LGLQARTSTSG*FCIFSGDGVSPCRSGW SRTPDLR*SAHLGLPKCWDYRHEPPRLARLRPF *ANILGPGSLSSKEVSSPKSSVLTMVVSLRALLL ALRQKPHSTGTIHEVTAQLMMSVRLSFWPVAVC SCVAQMASFILIKAPPQPQQSPQPVLSTLLFMY LPGPAR*HPVALHNTS*VAVSCNFGDRFLSFPLF FLSKAQRLTTSCSV*VFLTDRVGPHHPWREQRW QGDSLAQ*PWAQASPSRAFREAPSIFKDVKRHF FL*GPSPASYQGQIIEGYFLFWGTIIT*MIVLIK ILSWK

Frame 1

E*GR*QPRARVRARCSAAP*P*SAAERRAESGP LPSRGPVPRRPRHLRPESRSAGSRLHRRSRLLC PPCSRQGPSPNSPFRSGRVLAAHAGRWGPAQPRP FPGRAAGHHGGPEACAATPGGLKGSECRPWGAR VPGLSPAPWPGPSLPA*ASALSRGSNPRRSP*G SSPWTPRWSSIAR*SGKGRTSLIWCAGLWGSEKP GSLDCSTQSRTQWPGSKWTRRYWIMMFQRKNQS PFTSWPNFILRMLKRSWFRRSHNIYSSYR*RSR F*MKRSTALLRLLCSWLLTPSRPSSPCRAPSPEW PSLKSSSR*PSHPRTCHFSCYRDRGMDQISREP SIGSWLGVSVGAPELSPAE*RAQPLLCAIALNY DPVEISLMAF*FSDLSLCFKKYLSSFV*CGIPDS LRPRHRCVCVLHSRADLHRPAWEVIVGSETLKP *EASAIIPTPPRVPEHSFPHR*GPGMQRSSPAR RIPGQHACRLLIVREPAQSS*RASAGSPPGTPRR RSGWQLVGNRQGSCGWGETLGRSCDAG*LPAEG LGSAVRELMVQGTRCPPPATLDQGPRGRQEPLM SSPLPDAHRRSFLCGAGVLPGRTAVSTRNPAMTR DL*ALWLHRCCFSLAQTLSSTGPVCTAHLLHLP SQPRASGHSPLPNPLLYPGLTLLLPLSLFGP*I FCSLGASQGPLCPSQHRPDAGVHSQVALT*AVTG PTSMPGNRGPQNHPHGSLLGLGPPSCPFFFFFF FFFRDGVSLCHPGGSVVAQSRLTATSTS*VQAI LLPQPPK*LGLQACTTTLGQCSVFLIGTGFCHVS *AGLKLTPGITGMSHCTQPLLALSFLHG*CCCG QPGPLRSFQFGCYAKQVICLNQIKDRGYGGLKI FGEQRWSLAIPSGFEKLSSSQSTEILPSSYYQAD LMGLGCSATAWVTLPQGNQPWVPPSHLGSTGWD SRPRA*VWMLWPCVFLV*PSQERKLQAFVK*GL ALPPPFFFFFCFCFCFFFEMESRSVAQAGVQCHD LSSLQPPPPRYKRFSCLSLPSSWDYRRVPPCLA NFCIFSRDGVSPC*PDWSRTPDLR*SSHPGFPK FWDYRREPPCPVSSQS*SPSLDFHQELLSS*PVN LFFNNGCRWEHMVVYKNAVGLCFLL*LPCLLLK SGKCQATRPQGSWLVFELWQKHLGLQGAGWELQ DLAQPGALPPP*GRNTEVPPHRWRRWRGGWASGA SQAPVAGERKKEMPGFHRLVPVAGMTASFFFFF *DGVFAFVAQAGVQWRDLGSPQPPPPGFKQFSC LSLPSSWDYRHAPPRPANFVFLVETGFLHVGQAG LELPTSGDLPTSASQSAGITGMSHRAWPDCVLF KRTF*GLGVCQVRKSQAQRAAS*PWWFHYEPFC WLSGRSPTAPGPFMRSLPSS*CP*GCPFGQ*PCA AVWHRWLRSS*SRPHLSHSSPPNLCCPPYYSCT CQALLDSTPWHYITLHEWLCLVILGTGFSLSLS FFCQKPRD*QPAAVSKCSSLTGWGLTTPGGSSVG RETAWPSDPGPKPAPPGLSGKRHPFSKMSNVTS SCRARVLPPIRARS*KAIFYSGERL*LK*LF** KF*AGK

Frame 2

SEDGDSHARAYAPDAARPRDPSRPLRGARSLGR CRLGVPSRGVPGISGPNPGVPGRACTEGPGSCA LPAAVRARPPTPLSAQAGSSRPMLAAGDPRSPD RSRAGQPATMVALRPVQQLQGG*RAQSAGRGARG SRA*APRHGRGHRFPHELQLSQEEATQDVHRED RHHGRRDGVQLRDEVEREGPL*FGVPDSGAPRN LVLWTAVHNQGHSGLAQNGQEGTGS*CFKGRTSH LSLLGQILS*EC*RGAGSGDHTTFILLTGKEAD FR*KDLLPS*GFCAPGFLRRPGQAHLAERQVPS GLL*RALAGDPATPGPATSPATGTAGWTRYQESH P*GAGWGFPWELQNFPQLSEEPSPSYVQLP*TT TL*RFLSWRSSSLT*VFVLRSICLPLSNVGFLT PFVQGTGVCVSCTPELTSTAQPGKSL*GVRH*SP EKPVPSSPPRPGFRNIHSPTGEDLACSEAAQPG GSQASTPAGFSSSGSPPRARDEQVLGPRQAPRG GALAGSWWGIGRAAVAGERL*AEAVMQADCQPRG WVVPCGS*WYRALAVPLRPP*TRVREAGRSHSC LPHCPTPIDAPSCVGLGYSLVVLQSAPVTRL*P GICKPCGSTGAASHWPRL*APPAQSARPICFTFP PSHVPVATAHFPTHCCTQASLCCCPCPSSGPEF SVPWGPARALCAPPSTGLMQVSTHRWRSPRLSQ DPPPCQATEGHRTTPTAHSLVWGHLLALSFFFFF FFSEMESLSVTQVGVWWHNLGSLQPPPPEFKQF SCLSLPSSWDYRHAPPHLANVLYF**GRGFAML ARLVSNSHLGLQA*ATAPSPFLPFLFSMADAAVA SQGP*DPSSLAVMQSR*FVLIR*KIEAMGASRF LESRGGLWQFHLVLRNLAAHRAQRSCLLPTIRL T*WGWAARQLLGSPCPKETSPGCHPAT*GLQGGT PDLERKYGCCGPVSS*CDPARSGSFRRL*SEVW LCLLRFFFFSVSVSVFFLRWSLALSPRLECSVT ISAHCNLRLPGTRDSPASASRVAGTTGVCHHAWL IFVFLVEMGFRHVSQTGLELLTSGDPPTPASQS SGIIGVSHHARSLLSLEAHPWISTRSYFPPDL* ICSLTMAAGGSIWWFIKTLSGFVSFFSCRVYF*S LGSAKPRGLKGAGWCLSCGRSTWGSREQAGNCR TLLSQEHFPLLEAGILRCLPTDGEGGEEDGPQE HLKPQ*QEKERKRCLVFTDWFLWLG*LHPFFFFF ETEFLPLSPRLECNGVISAHRNLRLPDSSNSPA SASRVAGITGTHLHVRLILYF*WRRGFSMSVRL VSNSRPQVICPPRPPKVLGLQA*ATALGQTASFL SEHFRAWEFVK*GSLKPKEQRPDHGGFITSPSA GSQAEAPQHRDHS*GHCPAHDVREAVLLASSRV QLCGTDGFVHPDQGPTSATAVPPTCVVHPIIHVP ARPC*IAPRGIT*HFMSGCVL*FWGQVSLFPSL FFVKSPETDNQLQCLSVPH*QGGASPPLEGAAL AGRQPGPVTLGPSQPLQGFQGSAIHFQRCQTSLL PVGPESCLLSGPDHRRLFSILGNDYNLNDCFNK NSKLE

Sequence NM_181825

refseq_NF2.F2 (forward primer)
refseq_NF2.R2 (reverse primer)
Single exon skipping

Frame 0

Frame 1

Frame 2

Sequence NM_000268

GGAGTGAGGACGGTGACAGCCACGCGCGCGCGTACGCGCCCGATGCAGCGCGGCCCCGTGACCCTAGTCGGCCGCTGAGAGGCGCGCGGAGTCTGGGCCG CTGCCGTCTAGGGGTCCCGTCCCGAGGCGTCCCCGGCATCTCCGGCCCGAATCCCGGAGTGCCGGGTCGCGCCTGCACCGAAGGTCCCGGCTCCTGTGCC CTCCCTGCAGCCGTCAGGGCCCGTCCCCCAACTCCCCTTTCCGCTCAGGCAGGGTCCTCGCGGCCCATGCTGGCCGCTGGGGACCCGCGCAGCCCAGACC GTTCCCGGGCCGGGCAGCCGGCCACCATGGTGGCCCTGAGGCCTGTGCAGCAACTCCAGGGGGGCTAAAGGGCTCAGAGTGCAGGCCGTGGGGCGCGAGG GTCCCGGGCCTGAGCCCCGCGCCATGGCCGGGGCCATCGCTTCCCGCATGAGCTTCAGCTCTCTCAAGAGGAAGCAACCCAAGACGTTCACCGTGAGGAT CGTCACCATGGACGCCGAGATGGAGTTCAATTGCGAGATGAAGTGGAAAGGGAAGGACCTCTTTGATTTGGTGTGCCGGACTCTGGGGCTCCGAGAAACC TGGTTCTTTGGACTGCAGTACACAATCAAGGACACAGTGGCCTGGCTCAAAATGGACAAGAAGGTACTGGATCATGATGTTTCAAAGGAAGAACCAGTCA CCTTTCACTTCTTGGCCAAATTTTATCCTGAGAATGCTGAAGAGGAGCTGGTTCAGGAGATCACACAACATTTATTCTTCTTACAGGTAAAGAAGCAGAT TTTAGATGAAAAGATCTACTGCCCTCCTGAGGCTTCTGTGCTCCTGGCTTCTTACGCCGTCCAGGCCAAGTATGGTGACTACGACCCCAGTGTTCACAAG CGGGGATTTTTGGCCCAAGAGGAATTGCTTCCAAAAAGGGTAATAAATCTGTATCAGATGACTCCGGAAATGTGGGAGGAGAGAATTACTGCTTGGTACG CAGAGCACCGAGGCCGAGCCAGGGATGAAGCTGAAATGGAATATCTGAAGATAGCTCAGGACCTGGAGATGTACGGTGTGAACTACTTTGCAATCCGGAA TAAAAAGGGCACAGAGCTGCTGCTTGGAGTGGATGCCCTGGGGCTTCACATTTATGACCCTGAGAACAGACTGACCCCCAAGATCTCCTTCCCGTGGAAT GAAATCCGAAACATCTCGTACAGTGACAAGGAGTTTACTATTAAACCACTGGATAAGAAAATTGATGTCTTCAAGTTTAACTCCTCAAAGCTTCGTGTTA ATAAGCTGATTCTCCAGCTATGTATCGGGAACCATGATCTATTTATGAGGAGAAGGAAAGCCGATTCTTTGGAAGTTCAGCAGATGAAAGCCCAGGCCAG GGAGGAGAAGGCTAGAAAGCAGATGGAGCGGCAGCGCCTCGCTCGAGAGAAGCAGATGAGGGAGGAGGCTGAACGCACGAGGGATGAGTTGGAGAGGAGG CTGCTGCAGATGAAAGAAGAAGCAACAATGGCCAACGAAGCACTGATGCGGTCTGAGGAGACAGCTGACCTGTTGGCTGAAAAGGCCCAGATCACCGAGG AGGAGGCAAAACTTCTGGCCCAGAAGGCCGCAGAGGCTGAGCAGGAAATGCAGCGCATCAAGGCCACAGCGATTCGCACGGAGGAGGAGAAGCGCCTGAT GGAGCAGAAGGTGCTGGAAGCCGAGGTGCTGGCACTGAAGATGGCTGAGGAGTCAGAGAGGAGGGCCAAAGAGGCAGATCAGCTGAAGCAGGACCTGCAG GAAGCACGCGAGGCGGAGCGAAGAGCCAAGCAGAAGCTCCTGGAGATTGCCACCAAGCCCACGTACCCGCCCATGAACCCAATTCCAGCACCGTTGCCTC CTGACATACCAAGCTTCAACCTCATTGGTGACAGCCTGTCTTTCGACTTCAAAGATACTGACATGAAGCGGCTTTCCATGGAGATAGAGAAAGAAAAAGT GGAATACATGGAAAAGAGCAAGCATCTGCAGGAGCAGCTCAATGAACTCAAGACAGAAATCGAGGCCTTGAAACTGAAAGAGAGGGAGACAGCTCTGGAT ATTCTGCACAATGAGAACTCCGACAGGGGTGGCAGCAGCAAGCACAATACCATTAAAAAGCTCACCTTGCAGAGCGCCAAGTCCCGAGTGGCCTTCTTTG AAGAGCTCTAGCAGGTGACCCAGCCACCCCAGGACCTGCCACTTCTCCTGCTACCGGGACCGCGGGATGGACCAGATATCAAGAGAGCCATCCATAGGGA GCTGGCTGGGGGTTTCCGTGGGAGCTCCAGAACTTTCCCCAGCTGAGTGAAGAGCCCAGCCCCTCTTATGTGCAATTGCCTTGAACTACGACCCTGTAGA GATTTCTCTCATGGCGTTCTAGTTCTCTGACCTGAGTCTTTGTTTTAAGAAGTATTTGTCTTCCTTTGTCTAATGTGGGATTCCTGACTCCCTTCGTCCA AGGCACCGGTGTGTGTGTGTCTTGCACTCCAGAGCTGACCTCCACCGCCCAGCCTGGGAAGTCATTGTAGGGAGTGAGACACTGAAGCCCTGAGAAGCCA GTGCCATCATCCCCACCCCGCCCAGGGTTCCGGAACATTCATTCCCCCACCGGTGAGGACCTGGCATGCAGCGAAGCAGCCCAGCCCGGCGGATCCCAGG CCAGCACGCCTGCCGGCTTCTCATCGTCAGGGAGCCCGCCCAGAGCTCGTGACGAGCAAGTGCTGGGTCCCCGCCAGGCACCCCGAGGCGGCGCTCTGGC TGGCAGCTGGTGGGGAATAGGCAGGGCAGCTGTGGCTGGGGAGAGACTTTAGGCAGAAGCTGTGATGCAGGCTGACTGCCAGCCGAGGGGCTGGGTAGTG CCGTGCGGGAGCTGATGGTACAGGGCACTCGCTGTCCCCCTCCGGCCACCCTAGACCAGGGTCCGAGAGGCAGGCAGGAGCCACTCATGTCTTCCCCATT GCCCGACGCCCATAGACGCTCCTTCCTGTGTGGGGCTGGGGTACTCCCTGGTCGTACTGCAGTCAGCACCCGTAACCCGGCTATGACCAGGGATCTGTAA GCCCTGTGGCTCCACAGGTGCTGCTTCTCACTGGCCCAGACTCTGAGCTCCACCGGCCCAGTCTGCACGGCCCATCTGCTTCACCTTCCCTCCCAGCCAC GTGCCAGTGGCCACAGCCCACTTCCCAACCCACTGTTGTACCCAGGCCTCACTTTGCTGTTGCCCTTGTCCCTCTTCGGGCCCTGAATTTTCTGTTCCCT GGGGGCCAGCCAGGGCCCTTTGTGCCCCTCCCAGCACAGGCCTGATGCAGGTGTCCACTCACAGGTGGCGCTCACCTAGGCTGTCACAGGACCCACCTCC ATGCCAGGCAACAGAGGGCCACAGAACCACCCCCACGGCTCACTCCTTGGTCTGGGGCCACCTTCTTGCCCTTTCTTTTTTTTTTTTTTTTTTTTTTTCC GAGATGGAGTCTCTCTCTGTCACCCAGGTGGGAGTGTGGTGGCACAATCTCGGCTCACTGCAACCTCCACCTCCTGAGTTCAAGCAATTCTCCTGCCTCA GCCTCCCAAGTAGCTGGGACTACAGGCATGCACCACCACACTTGGCCAATGTTCTGTATTTTTAATAGGGACGGGGTTTTGCCATGTTAGCTAGGCTGGT CTCAAACTCACACCTGGGATTACAGGCATGAGCCACTGCACCCAGCCCCTTCTTGCCCTTTCTTTTCTCCATGGCTGATGCTGCTGTGGCCAGCCAGGGC CCTTGAGATCCTTCCAGTTTGGCTGTTATGCAAAGCAGGTGATTTGTCTTAATCAGATAAAAGATAGAGGCTATGGGGGCCTCAAGATTTTTGGAGAGCA GAGGTGGTCTCTGGCAATTCCATCTGGTTTTGAGAAACTTAGCAGCTCACAGAGCACAGAGATCCTGCCTTCTTCCTACTATCAGGCTGACCTAATGGGG TTGGGCTGCTCGGCAACTGCTTGGGTCACCTTGCCCCAAGGAAACCAGCCCTGGGTGCCACCCAGCCACTTAGGGTCTACAGGGTGGGACTCCAGACCTA GAGCGTAAGTATGGATGTTGTGGCCCTGTGTCTTCCTAGTGTGACCCAGCCAGGAGCGGAAGCTTCAGGCGTTTGTAAAGTGAGGTCTGGCTCTGCCTCC TCCGTTTTTTTTTTTTTTCTGTTTCTGTTTCTGTTTTTTTTTTGAGATGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGTCACGATCTCAGCTCA CTGCAACCTCCGCCTCCCAGGTACAAGAGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGTGTGCCACCATGCCTGGCTAATTTTTGTA TTTTTAGTAGAGATGGGGTTTCGCCATGTTAGCCAGACTGGTCTCGAACTCCTGACCTCAGGTGATCCTCCCACCCCGGCTTCCCAAAGTTCTGGGATTA TAGGCGTGAGCCACCATGCCCGGTCTCTTCTCAGTCTTGAAGCCCATCCCTGGATTTCCACCAGGAGTTACTTTCCTCCTGACCTGTAAATTTGTTCTTT AACAATGGCTGCAGGTGGGAGCATATGGTGGTTTATAAAAACGCTGTCGGGCTTTGTTTCCTTCTTTAGCTGCCGTGTCTACTTCTGAAGTCTGGGAAGT GCCAAGCCACGCGGCCTCAAGGGAGCTGGCTGGTGTTTGAGCTGTGGCAGAAGCACCTGGGGCTCCAGGGAGCAGGCTGGGAACTGCAGGACCTTGCTCA GCCAGGAGCACTTCCCCCTCCTTGAGGCAGGAATACTGAGGTGCCTCCCCACAGATGGAGAAGGTGGAGAGGAGGATGGGCCTCAGGAGCATCTCAAGCC CCAGTAGCAGGAGAAAGAAAGAAAGAGATGCCTGGTTTTCACAGACTGGTTCCTGTGGCTGGGATGACTGCATCCTTTTTTTTTTTTTTTTGAGACGGAG TTTTTGCCTTTGTCGCCCAGGCTGGAGTGCAATGGCGTGATCTCGGCTCACCGCAACCTCCGCCTCCCGGATTCAAGCAATTCTCCTGCCTCAGCCTCCC GAGTAGCTGGGATTACAGGCACGCACCTCCACGTCCGGCTAATTTTGTATTTTTAGTGGAGACGGGGTTTCTCCATGTCGGTCAGGCTGGTCTCGAACTC CCGACCTCAGGTGATCTGCCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCTTGGCCAGACTGCGTCCTTTTTAAGCGAACAT TTTAGGGCCTGGGAGTTTGTCAAGTAAGGAAGTCTCAAGCCCAAAGAGCAGCGTCCTGACCATGGTGGTTTCATTACGAGCCCTTCTGCTGGCTCTCAGG CAGAAGCCCCACAGCACCGGGACCATTCATGAGGTCACTGCCCAGCTCATGATGTCCGTGAGGCTGTCCTTTTGGCCAGTAGCCGTGTGCAGCTGTGTGG CACAGATGGCTTCGTTCATCCTGATCAAGGCCCCACCTCAGCCACAGCAGTCCCCCCAACCTGTGTTGTCCACCCTATTATTCATGTACCTGCCAGGCCC TGCTAGATAGCACCCCGTGGCATTACATAACACTTCATGAGTGGCTGTGTCTTGTAATTTTGGGGACAGGTTTCTCTCTTTCCCTCTCTTTTTTTTGTCA AAAGCCCAGAGACTGACAACCAGCTGCAGTGTCTAAGTGTTCCTCACTGACAGGGTGGGGCCTCACCACCCCTGGAGGGAGCAGCGTTGGCAGGGAGACA GCCTGGCCCAGTGACCCTGGGCCCAAGCCAGCCCCTCCAGGGCTTTCAGGGAAGCGCCATCCATTTTCAAAGATGTCAAACGTCACTTCTTCCTGTAGGG CCCGAGTCCTGCCTCCTATCAGGGCCAGATCATAGAAGGCTATTTTCTATTCTGGGGAACGATTATAACTTAAATGATTGTTTTAATAAAAATTCTAAGC TGGAAAA

refseq_NF2.F2 (forward primer)
refseq_NF2.R2 (reverse primer)
Single exon skipping

Frame 0

GVRTVTATRARTRPMQRGPVTLVGR*EARGVWA AAV*GSRPEASPASPARIPECRVAPAPKVPAPVP SLQPSGPVPQLPFPLRQGPRGPCWPLGTRAAQT VPGPGSRPPWWP*GLCSNSRGAKGLRVQAVGRE GPGPEPRAMAGAIASRMSFSSLKRKQPKTFTVRI VTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRET WFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPV TFHFLAKFYPENAEEELVQEITQHLFFLQVKKQI LDEKIYCPPEASVLLASYAVQAKYGDYDPSVHK RGFLAQEELLPKRVINLYQMTPEMWEERITAWY AEHRGRARDEAEMEYLKIAQDLEMYGVNYFAIRN KKGTELLLGVDALGLHIYDPENRLTPKISFPWN EIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRV NKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAR EEKARKQMERQRLAREKQMREEAERTRDELERR LLQMKEEATMANEALMRSEETADLLAEKAQITE EEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLM EQKVLEAEVLALKMAEESERRAKEADQLKQDLQ EAREAERRAKQKLLEIATKPTYPPMNPIPAPLP PDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKV EYMEKSKHLQEQLNELKTEIEALKLKERETALD ILHNENSDRGGSSKHNTIKKLTLQSAKSRVAFF EEL*QVTQPPQDLPLLLLPGPRDGPDIKRAIHRE LAGGFRGSSRTFPS*VKSPAPLMCNCLELRPCR DFSHGVLVL*PESLF*EVFVFLCLMWDS*LPSS KAPVCVCLALQS*PPPPSLGSHCRE*DTEALRSQ CHHPHPAQGSGTFIPPPVRTWHAAKQPSPADPR PARLPASHRQGARPELVTSKCWVPARHPEAALW LAAGGE*AGQLWLGRDFRQKL*CRLTASRGAG*C RAGADGTGHSLSPSGHPRPGSERQAGATHVFPI ARRP*TLLPVWGWGTPWSYCSQHP*PGYDQGSV SPVAPQVLLLTGPDSELHRPSLHGPSASPSLPAT CQWPQPTSQPTVVPRPHFAVALVPLRALNFLFP GGQPGPFVPLPAQA*CRCPLTGGAHLGCHRTHL HARQQRATEPPPRLTPWSGATFLPFLFFFFFFFP RWSLSLSPRWECGGTISAHCNLHLLSSSNSPAS ASQVAGTTGMHHHTWPMFCIFNRDGVLPC*LGW SQTHTWDYRHEPLHPAPSCPFFSPWLMLLWPARA LEILPVWLLCKAGDLS*SDKR*RLWGPQDFWRA EVVSGNSIWF*ET*QLTEHRDPAFFLLSG*PNG VGLLGNCLGHLAPRKPALGATQPLRVYRVGLQT* SVSMDVVALCLPSVTQPGAEASGVCKVRSGSAS SVFFFFLFLFLFFF*DGVSLCRPGWSAVSRSQL TATSASQVQEILLPQPPE*LGLQACATMPG*FLY F**RWGFAMLARLVSNS*PQVILPPRLPKVLGL *A*ATMPGLFSVLKPIPGFPPGVTFLLTCKFVL *QWLQVGAYGGL*KRCRALFPSLAAVSTSEVWEV PSHAASRELAGV*AVAEAPGAPGSRLGTAGPCS ARSTSPSLRQEY*GASPQMEKVERRMGLRSISS PSSRRKKERDAWFSQTGSCGWDDCILFFFFLRRS FCLCRPGWSAMA*SRLTATSASRIQAILLPQPP E*LGLQARTSTSG*FCIFSGDGVSPCRSGWSRT PDLR*SAHLGLPKCWDYRHEPPRLARLRPF*ANI LGPGSLSSKEVSSPKSSVLTMVVSLRALLLALR QKPHSTGTIHEVTAQLMMSVRLSFWPVAVCSCV AQMASFILIKAPPQPQQSPQPVLSTLLFMYLPGP AR*HPVALHNTS*VAVSCNFGDRFLSFPLFFLS KAQRLTTSCSV*VFLTDRVGPHHPWREQRWQGD SLAQ*PWAQASPSRAFREAPSIFKDVKRHFFL*G PSPASYQGQIIEGYFLFWGTIIT*MIVLIKILS WK

Frame 1

E*GR*QPRARVRARCSAAP*P*SAAERRAESGP LPSRGPVPRRPRHLRPESRSAGSRLHRRSRLLC PPCSRQGPSPNSPFRSGRVLAAHAGRWGPAQPRP FPGRAAGHHGGPEACAATPGGLKGSECRPWGAR VPGLSPAPWPGPSLPA*ASALSRGSNPRRSP*G SSPWTPRWSSIAR*SGKGRTSLIWCAGLWGSEKP GSLDCSTQSRTQWPGSKWTRRYWIMMFQRKNQS PFTSWPNFILRMLKRSWFRRSHNIYSSYR*RSR F*MKRSTALLRLLCSWLLTPSRPSMVTTTPVFTS GDFWPKRNCFQKG**ICIR*LRKCGRRELLLGT QSTEAEPGMKLKWNI*R*LRTWRCTV*TTLQSG IKRAQSCCLEWMPWGFTFMTLRTD*PPRSPSRGM KSETSRTVTRSLLLNHWIRKLMSSSLTPQSFVL IS*FSSYVSGTMIYL*GEGKPILWKFSR*KPRP GRRRLESRWSGSASLERSR*GRRLNARGMSWRGG CCR*KKKQQWPTKH*CGLRRQLTCWLKRPRSPR RRQNFWPRRPQRLSRKCSASRPQRFARRRRSA* WSRRCWKPRCWH*RWLRSQRGGPKRQIS*SRTCR KHARRSEEPSRSSWRLPPSPRTRP*TQFQHRCL LTYQASTSLVTACLSTSKILT*SGFPWR*RKKK WNTWKRASICRSSSMNSRQKSRP*N*KRGRQLWI FCTMRTPTGVAAASTIPLKSSPCRAPSPEWPSL KSSSR*PSHPRTCHFSCYRDRGMDQISREPSIG SWLGVSVGAPELSPAE*RAQPLLCAIALNYDPVE ISLMAF*FSDLSLCFKKYLSSFV*CGIPDSLRP RHRCVCVLHSRADLHRPAWEVIVGSETLKP*EA SAIIPTPPRVPEHSFPHR*GPGMQRSSPARRIPG QHACRLLIVREPAQSS*RASAGSPPGTPRRRSG WQLVGNRQGSCGWGETLGRSCDAG*LPAEGLGS AVRELMVQGTRCPPPATLDQGPRGRQEPLMSSPL PDAHRRSFLCGAGVLPGRTAVSTRNPAMTRDL* ALWLHRCCFSLAQTLSSTGPVCTAHLLHLPSQP RASGHSPLPNPLLYPGLTLLLPLSLFGP*IFCSL GASQGPLCPSQHRPDAGVHSQVALT*AVTGPTS MPGNRGPQNHPHGSLLGLGPPSCPFFFFFFFFF RDGVSLCHPGGSVVAQSRLTATSTS*VQAILLPQ PPK*LGLQACTTTLGQCSVFLIGTGFCHVS*AG LKLTPGITGMSHCTQPLLALSFLHG*CCCGQPG PLRSFQFGCYAKQVICLNQIKDRGYGGLKIFGEQ RWSLAIPSGFEKLSSSQSTEILPSSYYQADLMG LGCSATAWVTLPQGNQPWVPPSHLGSTGWDSRP RA*VWMLWPCVFLV*PSQERKLQAFVK*GLALPP PFFFFFCFCFCFFFEMESRSVAQAGVQCHDLSS LQPPPPRYKRFSCLSLPSSWDYRRVPPCLANFC IFSRDGVSPC*PDWSRTPDLR*SSHPGFPKFWDY RREPPCPVSSQS*SPSLDFHQELLSS*PVNLFF NNGCRWEHMVVYKNAVGLCFLL*LPCLLLKSGK CQATRPQGSWLVFELWQKHLGLQGAGWELQDLAQ PGALPPP*GRNTEVPPHRWRRWRGGWASGASQA PVAGERKKEMPGFHRLVPVAGMTASFFFFF*DG VFAFVAQAGVQWRDLGSPQPPPPGFKQFSCLSLP SSWDYRHAPPRPANFVFLVETGFLHVGQAGLEL PTSGDLPTSASQSAGITGMSHRAWPDCVLFKRT F*GLGVCQVRKSQAQRAAS*PWWFHYEPFCWLSG RSPTAPGPFMRSLPSS*CP*GCPFGQ*PCAAVW HRWLRSS*SRPHLSHSSPPNLCCPPYYSCTCQA LLDSTPWHYITLHEWLCLVILGTGFSLSLSFFCQ KPRD*QPAAVSKCSSLTGWGLTTPGGSSVGRET AWPSDPGPKPAPPGLSGKRHPFSKMSNVTSSCR ARVLPPIRARS*KAIFYSGERL*LK*LF**KF*A GK

Frame 2

SEDGDSHARAYAPDAARPRDPSRPLRGARSLGR CRLGVPSRGVPGISGPNPGVPGRACTEGPGSCA LPAAVRARPPTPLSAQAGSSRPMLAAGDPRSPD RSRAGQPATMVALRPVQQLQGG*RAQSAGRGARG SRA*APRHGRGHRFPHELQLSQEEATQDVHRED RHHGRRDGVQLRDEVEREGPL*FGVPDSGAPRN LVLWTAVHNQGHSGLAQNGQEGTGS*CFKGRTSH LSLLGQILS*EC*RGAGSGDHTTFILLTGKEAD FR*KDLLPS*GFCAPGFLRRPGQVW*LRPQCSQ AGIFGPRGIASKKGNKSVSDDSGNVGGENYCLVR RAPRPSQG*S*NGISEDSSGPGDVRCELLCNPE *KGHRAAAWSGCPGASHL*P*EQTDPQDLLPVE *NPKHLVQ*QGVYY*TTG*EN*CLQV*LLKASC* *ADSPAMYREP*SIYEEKESRFFGSSADESPGQ GGEG*KADGAAAPRSREADEGGG*THEG*VGEE AAADERRSNNGQRSTDAV*GDS*PVG*KGPDHRG GGKTSGPEGRRG*AGNAAHQGHSDSHGGGEAPD GAEGAGSRGAGTEDG*GVREEGQRGRSAEAGPA GSTRGGAKSQAEAPGDCHQAHVPAHEPNSSTVAS *HTKLQPHW*QPVFRLQRY*HEAAFHGDRERKS GIHGKEQASAGAAQ*TQDRNRGLETEREGDSSG YSAQ*ELRQGWQQQAQYH*KAHLAERQVPSGLL* RALAGDPATPGPATSPATGTAGWTRYQESHP*G AGWGFPWELQNFPQLSEEPSPSYVQLP*TTTL* RFLSWRSSSLT*VFVLRSICLPLSNVGFLTPFVQ GTGVCVSCTPELTSTAQPGKSL*GVRH*SPEKP VPSSPPRPGFRNIHSPTGEDLACSEAAQPGGSQ ASTPAGFSSSGSPPRARDEQVLGPRQAPRGGALA GSWWGIGRAAVAGERL*AEAVMQADCQPRGWVV PCGS*WYRALAVPLRPP*TRVREAGRSHSCLPH CPTPIDAPSCVGLGYSLVVLQSAPVTRL*PGICK PCGSTGAASHWPRL*APPAQSARPICFTFPPSH VPVATAHFPTHCCTQASLCCCPCPSSGPEFSVP WGPARALCAPPSTGLMQVSTHRWRSPRLSQDPPP CQATEGHRTTPTAHSLVWGHLLALSFFFFFFFS EMESLSVTQVGVWWHNLGSLQPPPPEFKQFSCL SLPSSWDYRHAPPHLANVLYF**GRGFAMLARLV SNSHLGLQA*ATAPSPFLPFLFSMADAAVASQG P*DPSSLAVMQSR*FVLIR*KIEAMGASRFLES RGGLWQFHLVLRNLAAHRAQRSCLLPTIRLT*WG WAARQLLGSPCPKETSPGCHPAT*GLQGGTPDL ERKYGCCGPVSS*CDPARSGSFRRL*SEVWLCL LRFFFFSVSVSVFFLRWSLALSPRLECSVTISAH CNLRLPGTRDSPASASRVAGTTGVCHHAWLIFV FLVEMGFRHVSQTGLELLTSGDPPTPASQSSGI IGVSHHARSLLSLEAHPWISTRSYFPPDL*ICSL TMAAGGSIWWFIKTLSGFVSFFSCRVYF*SLGS AKPRGLKGAGWCLSCGRSTWGSREQAGNCRTLL SQEHFPLLEAGILRCLPTDGEGGEEDGPQEHLKP Q*QEKERKRCLVFTDWFLWLG*LHPFFFFFETE FLPLSPRLECNGVISAHRNLRLPDSSNSPASAS RVAGITGTHLHVRLILYF*WRRGFSMSVRLVSNS RPQVICPPRPPKVLGLQA*ATALGQTASFLSEH FRAWEFVK*GSLKPKEQRPDHGGFITSPSAGSQ AEAPQHRDHS*GHCPAHDVREAVLLASSRVQLCG TDGFVHPDQGPTSATAVPPTCVVHPIIHVPARP C*IAPRGIT*HFMSGCVL*FWGQVSLFPSLFFV KSPETDNQLQCLSVPH*QGGASPPLEGAALAGRQ PGPVTLGPSQPLQGFQGSAIHFQRCQTSLLPVG PESCLLSGPDHRRLFSILGNDYNLNDCFNKNSK LE

Sequence NM_016418

GGAGTGAGGACGGTGACAGCCACGCGCGCGCGTACGCGCCCGATGCAGCGCGGCCCCGTGACCCTAGTCGGCCGCTGAGAGGCGCGCGGAGTCTGGGCCG CTGCCGTCTAGGGGTCCCGTCCCGAGGCGTCCCCGGCATCTCCGGCCCGAATCCCGGAGTGCCGGGTCGCGCCTGCACCGAAGGTCCCGGCTCCTGTGCC CTCCCTGCAGCCGTCAGGGCCCGTCCCCCAACTCCCCTTTCCGCTCAGGCAGGGTCCTCGCGGCCCATGCTGGCCGCTGGGGACCCGCGCAGCCCAGACC GTTCCCGGGCCGGGCAGCCGGCCACCATGGTGGCCCTGAGGCCTGTGCAGCAACTCCAGGGGGGCTAAAGGGCTCAGAGTGCAGGCCGTGGGGCGCGAGG GTCCCGGGCCTGAGCCCCGCGCCATGGCCGGGGCCATCGCTTCCCGCATGAGCTTCAGCTCTCTCAAGAGGAAGCAACCCAAGACGTTCACCGTGAGGAT CGTCACCATGGACGCCGAGATGGAGTTCAATTGCGAGATGAAGTGGAAAGGGAAGGACCTCTTTGATTTGGTGTGCCGGACTCTGGGGCTCCGAGAAACC TGGTTCTTTGGACTGCAGTACACAATCAAGGACACAGTGGCCTGGCTCAAAATGGACAAGAAGGTACTGGATCATGATGTTTCAAAGGAAGAACCAGTCA CCTTTCACTTCTTGGCCAAATTTTATCCTGAGAATGCTGAAGAGGAGCTGGTTCAGGAGATCACACAACATTTATTCTTCTTACAGGTAAAGAAGCAGAT TTTAGATGAAAAGATCTACTGCCCTCCTGAGGCTTCTGTGCTCCTGGCTTCTTACGCCGTCCAGGCCAAGTATGGTGACTACGACCCCAGTGTTCACAAG CGGGGATTTTTGGCCCAAGAGGAATTGCTTCCAAAAAGGGTAATAAATCTGTATCAGATGACTCCGGAAATGTGGGAGGAGAGAATTACTGCTTGGTACG CAGAGCACCGAGGCCGAGCCAGGGATGAAGCTGAAATGGAATATCTGAAGATAGCTCAGGACCTGGAGATGTACGGTGTGAACTACTTTGCAATCCGGAA TAAAAAGGGCACAGAGCTGCTGCTTGGAGTGGATGCCCTGGGGCTTCACATTTATGACCCTGAGAACAGACTGACCCCCAAGATCTCCTTCCCGTGGAAT GAAATCCGAAACATCTCGTACAGTGACAAGGAGTTTACTATTAAACCACTGGATAAGAAAATTGATGTCTTCAAGTTTAACTCCTCAAAGCTTCGTGTTA ATAAGCTGATTCTCCAGCTATGTATCGGGAACCATGATCTATTTATGAGGAGAAGGAAAGCCGATTCTTTGGAAGTTCAGCAGATGAAAGCCCAGGCCAG GGAGGAGAAGGCTAGAAAGCAGATGGAGCGGCAGCGCCTCGCTCGAGAGAAGCAGATGAGGGAGGAGGCTGAACGCACGAGGGATGAGTTGGAGAGGAGG CTGCTGCAGATGAAAGAAGAAGCAACAATGGCCAACGAAGCACTGATGCGGTCTGAGGAGACAGCTGACCTGTTGGCTGAAAAGGCCCAGATCACCGAGG AGGAGGCAAAACTTCTGGCCCAGAAGGCCGCAGAGGCTGAGCAGGAAATGCAGCGCATCAAGGCCACAGCGATTCGCACGGAGGAGGAGAAGCGCCTGAT GGAGCAGAAGGTGCTGGAAGCCGAGGTGCTGGCACTGAAGATGGCTGAGGAGTCAGAGAGGAGGGCCAAAGAGGCAGATCAGCTGAAGCAGGACCTGCAG GAAGCACGCGAGGCGGAGCGAAGAGCCAAGCAGAAGCTCCTGGAGATTGCCACCAAGCCCACGTACCCGCCCATGAACCCAATTCCAGCACCGTTGCCTC CTGACATACCAAGCTTCAACCTCATTGGTGACAGCCTGTCTTTCGACTTCAAAGATACTGACATGAAGCGGCTTTCCATGGAGATAGAGAAAGAAAAAGT GGAATACATGGAAAAGAGCAAGCATCTGCAGGAGCAGCTCAATGAACTCAAGACAGAAATCGAGGCCTTGAAACTGAAAGAGAGGGAGACAGCTCTGGAT ATTCTGCACAATGAGAACTCCGACAGGGGTGGCAGCAGCAAGCACAATACCATTAAAAAGCCTCAAGCCCAAGGCAGAAGACCTATCTGCATTTGAGCCC TCAAACTCACCTTGCAGAGCGCCAAGTCCCGAGTGGCCTTCTTTGAAGAGCTCTAGCAGGTGACCCAGCCACCCCAGGACCTGCCACTTCTCCTGCTACC GGGACCGCGGGATGGACCAGATATCAAGAGAGCCATCCATAGGGAGCTGGCTGGGGGTTTCCGTGGGAGCTCCAGAACTTTCCCCAGCTGAGTGAAGAGC CCAGCCCCTCTTATGTGCAATTGCCTTGAACTACGACCCTGTAGAGATTTCTCTCATGGCGTTCTAGTTCTCTGACCTGAGTCTTTGTTTTAAGAAGTAT TTGTCTTCCTTTGTCTAATGTGGGATTCCTGACTCCCTTCGTCCAAGGCACCGGTGTGTGTGTGTCTTGCACTCCAGAGCTGACCTCCACCGCCCAGCCT GGGAAGTCATTGTAGGGAGTGAGACACTGAAGCCCTGAGAAGCCAGTGCCATCATCCCCACCCCGCCCAGGGTTCCGGAACATTCATTCCCCCACCGGTG AGGACCTGGCATGCAGCGAAGCAGCCCAGCCCGGCGGATCCCAGGCCAGCACGCCTGCCGGCTTCTCATCGTCAGGGAGCCCGCCCAGAGCTCGTGACGA GCAAGTGCTGGGTCCCCGCCAGGCACCCCGAGGCGGCGCTCTGGCTGGCAGCTGGTGGGGAATAGGCAGGGCAGCTGTGGCTGGGGAGAGACTTTAGGCA GAAGCTGTGATGCAGGCTGACTGCCAGCCGAGGGGCTGGGTAGTGCCGTGCGGGAGCTGATGGTACAGGGCACTCGCTGTCCCCCTCCGGCCACCCTAGA CCAGGGTCCGAGAGGCAGGCAGGAGCCACTCATGTCTTCCCCATTGCCCGACGCCCATAGACGCTCCTTCCTGTGTGGGGCTGGGGTACTCCCTGGTCGT ACTGCAGTCAGCACCCGTAACCCGGCTATGACCAGGGATCTGTAAGCCCTGTGGCTCCACAGGTGCTGCTTCTCACTGGCCCAGACTCTGAGCTCCACCG GCCCAGTCTGCACGGCCCATCTGCTTCACCTTCCCTCCCAGCCACGTGCCAGTGGCCACAGCCCACTTCCCAACCCACTGTTGTACCCAGGCCTCACTTT GCTGTTGCCCTTGTCCCTCTTCGGGCCCTGAATTTTCTGTTCCCTGGGGGCCAGCCAGGGCCCTTTGTGCCCCTCCCAGCACAGGCCTGATGCAGGTGTC CACTCACAGGTGGCGCTCACCTAGGCTGTCACAGGACCCACCTCCATGCCAGGCAACAGAGGGCCACAGAACCACCCCCACGGCTCACTCCTTGGTCTGG GGCCACCTTCTTGCCCTTTCTTTTTTTTTTTTTTTTTTTTTTTCCGAGATGGAGTCTCTCTCTGTCACCCAGGTGGGAGTGTGGTGGCACAATCTCGGCT CACTGCAACCTCCACCTCCTGAGTTCAAGCAATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGCATGCACCACCACACTTGGCCAATGTTCT GTATTTTTAATAGGGACGGGGTTTTGCCATGTTAGCTAGGCTGGTCTCAAACTCACACCTGGGATTACAGGCATGAGCCACTGCACCCAGCCCCTTCTTG CCCTTTCTTTTCTCCATGGCTGATGCTGCTGTGGCCAGCCAGGGCCCTTGAGATCCTTCCAGTTTGGCTGTTATGCAAAGCAGGTGATTTGTCTTAATCA GATAAAAGATAGAGGCTATGGGGGCCTCAAGATTTTTGGAGAGCAGAGGTGGTCTCTGGCAATTCCATCTGGTTTTGAGAAACTTAGCAGCTCACAGAGC ACAGAGATCCTGCCTTCTTCCTACTATCAGGCTGACCTAATGGGGTTGGGCTGCTCGGCAACTGCTTGGGTCACCTTGCCCCAAGGAAACCAGCCCTGGG TGCCACCCAGCCACTTAGGGTCTACAGGGTGGGACTCCAGACCTAGAGCGTAAGTATGGATGTTGTGGCCCTGTGTCTTCCTAGTGTGACCCAGCCAGGA GCGGAAGCTTCAGGCGTTTGTAAAGTGAGGTCTGGCTCTGCCTCCTCCGTTTTTTTTTTTTTTCTGTTTCTGTTTCTGTTTTTTTTTTGAGATGGAGTCT CGCTCTGTCGCCCAGGCTGGAGTGCAGTGTCACGATCTCAGCTCACTGCAACCTCCGCCTCCCAGGTACAAGAGATTCTCCTGCCTCAGCCTCCCGAGTA GCTGGGACTACAGGCGTGTGCCACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCGCCATGTTAGCCAGACTGGTCTCGAACTCCTGA CCTCAGGTGATCCTCCCACCCCGGCTTCCCAAAGTTCTGGGATTATAGGCGTGAGCCACCATGCCCGGTCTCTTCTCAGTCTTGAAGCCCATCCCTGGAT TTCCACCAGGAGTTACTTTCCTCCTGACCTGTAAATTTGTTCTTTAACAATGGCTGCAGGTGGGAGCATATGGTGGTTTATAAAAACGCTGTCGGGCTTT GTTTCCTTCTTTAGCTGCCGTGTCTACTTCTGAAGTCTGGGAAGTGCCAAGCCACGCGGCCTCAAGGGAGCTGGCTGGTGTTTGAGCTGTGGCAGAAGCA CCTGGGGCTCCAGGGAGCAGGCTGGGAACTGCAGGACCTTGCTCAGCCAGGAGCACTTCCCCCTCCTTGAGGCAGGAATACTGAGGTGCCTCCCCACAGA TGGAGAAGGTGGAGAGGAGGATGGGCCTCAGGAGCATCTCAAGCCCCAGTAGCAGGAGAAAGAAAGAAAGAGATGCCTGGTTTTCACAGACTGGTTCCTG TGGCTGGGATGACTGCATCCTTTTTTTTTTTTTTTTGAGACGGAGTTTTTGCCTTTGTCGCCCAGGCTGGAGTGCAATGGCGTGATCTCGGCTCACCGCA ACCTCCGCCTCCCGGATTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCACGCACCTCCACGTCCGGCTAATTTTGTATTTTTA GTGGAGACGGGGTTTCTCCATGTCGGTCAGGCTGGTCTCGAACTCCCGACCTCAGGTGATCTGCCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGCA TGAGCCACCGCGCTTGGCCAGACTGCGTCCTTTTTAAGCGAACATTTTAGGGCCTGGGAGTTTGTCAAGTAAGGAAGTCTCAAGCCCAAAGAGCAGCGTC CTGACCATGGTGGTTTCATTACGAGCCCTTCTGCTGGCTCTCAGGCAGAAGCCCCACAGCACCGGGACCATTCATGAGGTCACTGCCCAGCTCATGATGT CCGTGAGGCTGTCCTTTTGGCCAGTAGCCGTGTGCAGCTGTGTGGCACAGATGGCTTCGTTCATCCTGATCAAGGCCCCACCTCAGCCACAGCAGTCCCC CCAACCTGTGTTGTCCACCCTATTATTCATGTACCTGCCAGGCCCTGCTAGATAGCACCCCGTGGCATTACATAACACTTCATGAGTGGCTGTGTCTTGT AATTTTGGGGACAGGTTTCTCTCTTTCCCTCTCTTTTTTTTGTCAAAAGCCCAGAGACTGACAACCAGCTGCAGTGTCTAAGTGTTCCTCACTGACAGGG TGGGGCCTCACCACCCCTGGAGGGAGCAGCGTTGGCAGGGAGACAGCCTGGCCCAGTGACCCTGGGCCCAAGCCAGCCCCTCCAGGGCTTTCAGGGAAGC GCCATCCATTTTCAAAGATGTCAAACGTCACTTCTTCCTGTAGGGCCCGAGTCCTGCCTCCTATCAGGGCCAGATCATAGAAGGCTATTTTCTATTCTGG GGAACGATTATAACTTAAATGATTGTTTTAATAAAAATTCTAAGCTGGAAAA

refseq_NF2.F2 (forward primer)
refseq_NF2.R2 (reverse primer)
Single exon skipping

Frame 0

GVRTVTATRARTRPMQRGPVTLVGR*EARGVWA AAV*GSRPEASPASPARIPECRVAPAPKVPAPVP SLQPSGPVPQLPFPLRQGPRGPCWPLGTRAAQT VPGPGSRPPWWP*GLCSNSRGAKGLRVQAVGRE GPGPEPRAMAGAIASRMSFSSLKRKQPKTFTVRI VTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRET WFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPV TFHFLAKFYPENAEEELVQEITQHLFFLQVKKQI LDEKIYCPPEASVLLASYAVQAKYGDYDPSVHK RGFLAQEELLPKRVINLYQMTPEMWEERITAWY AEHRGRARDEAEMEYLKIAQDLEMYGVNYFAIRN KKGTELLLGVDALGLHIYDPENRLTPKISFPWN EIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRV NKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAR EEKARKQMERQRLAREKQMREEAERTRDELERR LLQMKEEATMANEALMRSEETADLLAEKAQITE EEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLM EQKVLEAEVLALKMAEESERRAKEADQLKQDLQ EAREAERRAKQKLLEIATKPTYPPMNPIPAPLP PDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKV EYMEKSKHLQEQLNELKTEIEALKLKERETALD ILHNENSDRGGSSKHNTIKKPQAQGRRPICI*A LKLTLQSAKSRVAFFEEL*QVTQPPQDLPLLLLP GPRDGPDIKRAIHRELAGGFRGSSRTFPS*VKS PAPLMCNCLELRPCRDFSHGVLVL*PESLF*EV FVFLCLMWDS*LPSSKAPVCVCLALQS*PPPPSL GSHCRE*DTEALRSQCHHPHPAQGSGTFIPPPV RTWHAAKQPSPADPRPARLPASHRQGARPELVT SKCWVPARHPEAALWLAAGGE*AGQLWLGRDFRQ KL*CRLTASRGAG*CRAGADGTGHSLSPSGHPR PGSERQAGATHVFPIARRP*TLLPVWGWGTPWS YCSQHP*PGYDQGSVSPVAPQVLLLTGPDSELHR PSLHGPSASPSLPATCQWPQPTSQPTVVPRPHF AVALVPLRALNFLFPGGQPGPFVPLPAQA*CRC PLTGGAHLGCHRTHLHARQQRATEPPPRLTPWSG ATFLPFLFFFFFFFPRWSLSLSPRWECGGTISA HCNLHLLSSSNSPASASQVAGTTGMHHHTWPMF CIFNRDGVLPC*LGWSQTHTWDYRHEPLHPAPSC PFFSPWLMLLWPARALEILPVWLLCKAGDLS*S DKR*RLWGPQDFWRAEVVSGNSIWF*ET*QLTE HRDPAFFLLSG*PNGVGLLGNCLGHLAPRKPALG ATQPLRVYRVGLQT*SVSMDVVALCLPSVTQPG AEASGVCKVRSGSASSVFFFFLFLFLFFF*DGV SLCRPGWSAVSRSQLTATSASQVQEILLPQPPE* LGLQACATMPG*FLYF**RWGFAMLARLVSNS* PQVILPPRLPKVLGL*A*ATMPGLFSVLKPIPG FPPGVTFLLTCKFVL*QWLQVGAYGGL*KRCRAL FPSLAAVSTSEVWEVPSHAASRELAGV*AVAEA PGAPGSRLGTAGPCSARSTSPSLRQEY*GASPQ MEKVERRMGLRSISSPSSRRKKERDAWFSQTGSC GWDDCILFFFFLRRSFCLCRPGWSAMA*SRLTA TSASRIQAILLPQPPE*LGLQARTSTSG*FCIF SGDGVSPCRSGWSRTPDLR*SAHLGLPKCWDYRH EPPRLARLRPF*ANILGPGSLSSKEVSSPKSSV LTMVVSLRALLLALRQKPHSTGTIHEVTAQLMM SVRLSFWPVAVCSCVAQMASFILIKAPPQPQQSP QPVLSTLLFMYLPGPAR*HPVALHNTS*VAVSC NFGDRFLSFPLFFLSKAQRLTTSCSV*VFLTDR VGPHHPWREQRWQGDSLAQ*PWAQASPSRAFREA PSIFKDVKRHFFL*GPSPASYQGQIIEGYFLFW GTIIT*MIVLIKILSWK

Frame 1

E*GR*QPRARVRARCSAAP*P*SAAERRAESGP LPSRGPVPRRPRHLRPESRSAGSRLHRRSRLLC PPCSRQGPSPNSPFRSGRVLAAHAGRWGPAQPRP FPGRAAGHHGGPEACAATPGGLKGSECRPWGAR VPGLSPAPWPGPSLPA*ASALSRGSNPRRSP*G SSPWTPRWSSIAR*SGKGRTSLIWCAGLWGSEKP GSLDCSTQSRTQWPGSKWTRRYWIMMFQRKNQS PFTSWPNFILRMLKRSWFRRSHNIYSSYR*RSR F*MKRSTALLRLLCSWLLTPSRPSMVTTTPVFTS GDFWPKRNCFQKG**ICIR*LRKCGRRELLLGT QSTEAEPGMKLKWNI*R*LRTWRCTV*TTLQSG IKRAQSCCLEWMPWGFTFMTLRTD*PPRSPSRGM KSETSRTVTRSLLLNHWIRKLMSSSLTPQSFVL IS*FSSYVSGTMIYL*GEGKPILWKFSR*KPRP GRRRLESRWSGSASLERSR*GRRLNARGMSWRGG CCR*KKKQQWPTKH*CGLRRQLTCWLKRPRSPR RRQNFWPRRPQRLSRKCSASRPQRFARRRRSA* WSRRCWKPRCWH*RWLRSQRGGPKRQIS*SRTCR KHARRSEEPSRSSWRLPPSPRTRP*TQFQHRCL LTYQASTSLVTACLSTSKILT*SGFPWR*RKKK WNTWKRASICRSSSMNSRQKSRP*N*KRGRQLWI FCTMRTPTGVAAASTIPLKSLKPKAEDLSAFEP SNSPCRAPSPEWPSLKSSSR*PSHPRTCHFSCY RDRGMDQISREPSIGSWLGVSVGAPELSPAE*RA QPLLCAIALNYDPVEISLMAF*FSDLSLCFKKY LSSFV*CGIPDSLRPRHRCVCVLHSRADLHRPA WEVIVGSETLKP*EASAIIPTPPRVPEHSFPHR* GPGMQRSSPARRIPGQHACRLLIVREPAQSS*R ASAGSPPGTPRRRSGWQLVGNRQGSCGWGETLG RSCDAG*LPAEGLGSAVRELMVQGTRCPPPATLD QGPRGRQEPLMSSPLPDAHRRSFLCGAGVLPGR TAVSTRNPAMTRDL*ALWLHRCCFSLAQTLSST GPVCTAHLLHLPSQPRASGHSPLPNPLLYPGLTL LLPLSLFGP*IFCSLGASQGPLCPSQHRPDAGV HSQVALT*AVTGPTSMPGNRGPQNHPHGSLLGL GPPSCPFFFFFFFFFRDGVSLCHPGGSVVAQSRL TATSTS*VQAILLPQPPK*LGLQACTTTLGQCS VFLIGTGFCHVS*AGLKLTPGITGMSHCTQPLL ALSFLHG*CCCGQPGPLRSFQFGCYAKQVICLNQ IKDRGYGGLKIFGEQRWSLAIPSGFEKLSSSQS TEILPSSYYQADLMGLGCSATAWVTLPQGNQPW VPPSHLGSTGWDSRPRA*VWMLWPCVFLV*PSQE RKLQAFVK*GLALPPPFFFFFCFCFCFFFEMES RSVAQAGVQCHDLSSLQPPPPRYKRFSCLSLPS SWDYRRVPPCLANFCIFSRDGVSPC*PDWSRTPD LR*SSHPGFPKFWDYRREPPCPVSSQS*SPSLD FHQELLSS*PVNLFFNNGCRWEHMVVYKNAVGL CFLL*LPCLLLKSGKCQATRPQGSWLVFELWQKH LGLQGAGWELQDLAQPGALPPP*GRNTEVPPHR WRRWRGGWASGASQAPVAGERKKEMPGFHRLVP VAGMTASFFFFF*DGVFAFVAQAGVQWRDLGSPQ PPPPGFKQFSCLSLPSSWDYRHAPPRPANFVFL VETGFLHVGQAGLELPTSGDLPTSASQSAGITG MSHRAWPDCVLFKRTF*GLGVCQVRKSQAQRAAS *PWWFHYEPFCWLSGRSPTAPGPFMRSLPSS*C P*GCPFGQ*PCAAVWHRWLRSS*SRPHLSHSSP PNLCCPPYYSCTCQALLDSTPWHYITLHEWLCLV ILGTGFSLSLSFFCQKPRD*QPAAVSKCSSLTG WGLTTPGGSSVGRETAWPSDPGPKPAPPGLSGK RHPFSKMSNVTSSCRARVLPPIRARS*KAIFYSG ERL*LK*LF**KF*AGK

Frame 2

SEDGDSHARAYAPDAARPRDPSRPLRGARSLGR CRLGVPSRGVPGISGPNPGVPGRACTEGPGSCA LPAAVRARPPTPLSAQAGSSRPMLAAGDPRSPD RSRAGQPATMVALRPVQQLQGG*RAQSAGRGARG SRA*APRHGRGHRFPHELQLSQEEATQDVHRED RHHGRRDGVQLRDEVEREGPL*FGVPDSGAPRN LVLWTAVHNQGHSGLAQNGQEGTGS*CFKGRTSH LSLLGQILS*EC*RGAGSGDHTTFILLTGKEAD FR*KDLLPS*GFCAPGFLRRPGQVW*LRPQCSQ AGIFGPRGIASKKGNKSVSDDSGNVGGENYCLVR RAPRPSQG*S*NGISEDSSGPGDVRCELLCNPE *KGHRAAAWSGCPGASHL*P*EQTDPQDLLPVE *NPKHLVQ*QGVYY*TTG*EN*CLQV*LLKASC* *ADSPAMYREP*SIYEEKESRFFGSSADESPGQ GGEG*KADGAAAPRSREADEGGG*THEG*VGEE AAADERRSNNGQRSTDAV*GDS*PVG*KGPDHRG GGKTSGPEGRRG*AGNAAHQGHSDSHGGGEAPD GAEGAGSRGAGTEDG*GVREEGQRGRSAEAGPA GSTRGGAKSQAEAPGDCHQAHVPAHEPNSSTVAS *HTKLQPHW*QPVFRLQRY*HEAAFHGDRERKS GIHGKEQASAGAAQ*TQDRNRGLETEREGDSSG YSAQ*ELRQGWQQQAQYH*KASSPRQKTYLHLSP QTHLAERQVPSGLL*RALAGDPATPGPATSPAT GTAGWTRYQESHP*GAGWGFPWELQNFPQLSEE PSPSYVQLP*TTTL*RFLSWRSSSLT*VFVLRSI CLPLSNVGFLTPFVQGTGVCVSCTPELTSTAQP GKSL*GVRH*SPEKPVPSSPPRPGFRNIHSPTG EDLACSEAAQPGGSQASTPAGFSSSGSPPRARDE QVLGPRQAPRGGALAGSWWGIGRAAVAGERL*A EAVMQADCQPRGWVVPCGS*WYRALAVPLRPP* TRVREAGRSHSCLPHCPTPIDAPSCVGLGYSLVV LQSAPVTRL*PGICKPCGSTGAASHWPRL*APP AQSARPICFTFPPSHVPVATAHFPTHCCTQASL CCCPCPSSGPEFSVPWGPARALCAPPSTGLMQVS THRWRSPRLSQDPPPCQATEGHRTTPTAHSLVW GHLLALSFFFFFFFSEMESLSVTQVGVWWHNLG SLQPPPPEFKQFSCLSLPSSWDYRHAPPHLANVL YF**GRGFAMLARLVSNSHLGLQA*ATAPSPFL PFLFSMADAAVASQGP*DPSSLAVMQSR*FVLI R*KIEAMGASRFLESRGGLWQFHLVLRNLAAHRA QRSCLLPTIRLT*WGWAARQLLGSPCPKETSPG CHPAT*GLQGGTPDLERKYGCCGPVSS*CDPAR SGSFRRL*SEVWLCLLRFFFFSVSVSVFFLRWSL ALSPRLECSVTISAHCNLRLPGTRDSPASASRV AGTTGVCHHAWLIFVFLVEMGFRHVSQTGLELL TSGDPPTPASQSSGIIGVSHHARSLLSLEAHPWI STRSYFPPDL*ICSLTMAAGGSIWWFIKTLSGF VSFFSCRVYF*SLGSAKPRGLKGAGWCLSCGRS TWGSREQAGNCRTLLSQEHFPLLEAGILRCLPTD GEGGEEDGPQEHLKPQ*QEKERKRCLVFTDWFL WLG*LHPFFFFFETEFLPLSPRLECNGVISAHR NLRLPDSSNSPASASRVAGITGTHLHVRLILYF* WRRGFSMSVRLVSNSRPQVICPPRPPKVLGLQA *ATALGQTASFLSEHFRAWEFVK*GSLKPKEQR PDHGGFITSPSAGSQAEAPQHRDHS*GHCPAHDV REAVLLASSRVQLCGTDGFVHPDQGPTSATAVP PTCVVHPIIHVPARPC*IAPRGIT*HFMSGCVL *FWGQVSLFPSLFFVKSPETDNQLQCLSVPH*QG GASPPLEGAALAGRQPGPVTLGPSQPLQGFQGS AIHFQRCQTSLLPVGPESCLLSGPDHRRLFSIL GNDYNLNDCFNKNSKLE

Sequence NM_181832

GGAGTGAGGACGGTGACAGCCACGCGCGCGCGTACGCGCCCGATGCAGCGCGGCCCCGTGACCCTAGTCGGCCGCTGAGAGGCGCGCGGAGTCTGGGCCG CTGCCGTCTAGGGGTCCCGTCCCGAGGCGTCCCCGGCATCTCCGGCCCGAATCCCGGAGTGCCGGGTCGCGCCTGCACCGAAGGTCCCGGCTCCTGTGCC CTCCCTGCAGCCGTCAGGGCCCGTCCCCCAACTCCCCTTTCCGCTCAGGCAGGGTCCTCGCGGCCCATGCTGGCCGCTGGGGACCCGCGCAGCCCAGACC GTTCCCGGGCCGGGCAGCCGGCCACCATGGTGGCCCTGAGGCCTGTGCAGCAACTCCAGGGGGGCTAAAGGGCTCAGAGTGCAGGCCGTGGGGCGCGAGG GTCCCGGGCCTGAGCCCCGCGCCATGGCCGGGGCCATCGCTTCCCGCATGAGCTTCAGCTCTCTCAAGAGGAAGCAACCCAAGACGTTCACCGTGAGGAT CGTCACCATGGACGCCGAGATGGAGTTCAATTGCGAGATGAAGTGGAAAGGGAAGGACCTCTTTGATTTGGTGTGCCGGACTCTGGGGCTCCGAGAAACC TGGTTCTTTGGACTGCAGTACACAATCAAGGACACAGTGGCCTGGCTCAAAATGGACAAGAAGGTACTGGATCATGATGTTTCAAAGGAAGAACCAGTCA CCTTTCACTTCTTGGCCAAATTTTATCCTGAGAATGCTGAAGAGGAGCTGGTTCAGGAGATCACACAACATTTATTCTTCTTACAGGTAAAGAAGCAGAT TTTAGATGAAAAGATCTACTGCCCTCCTGAGGCTTCTGTGCTCCTGGCTTCTTACGCCGTCCAGGCCAAGTATGGTGACTACGACCCCAGTGTTCACAAG CGGGGATTTTTGGCCCAAGAGGAATTGCTTCCAAAAAGGGTAATAAATCTGTATCAGATGACTCCGGAAATGTGGGAGGAGAGAATTACTGCTTGGTACG CAGAGCACCGAGGCCGAGCCAGGGATGAAGCTGAAATGGAATATCTGAAGATAGCTCAGGACCTGGAGATGTACGGTGTGAACTACTTTGCAATCCGGAA TAAAAAGGGCACAGAGCTGCTGCTTGGAGTGGATGCCCTGGGGCTTCACATTTATGACCCTGAGAACAGACTGACCCCCAAGATCTCCTTCCCGTGGAAT GAAATCCGAAACATCTCGTACAGTGACAAGGAGTTTACTATTAAACCACTGGATAAGAAAATTGATGTCTTCAAGTTTAACTCCTCAAAGCTTCGTGTTA ATAAGCTGATTCTCCAGCTATGTATCGGGAACCATGATCTATTTATGAGGAGAAGGAAAGCCGATTCTTTGGAAGTTCAGCAGATGAAAGCCCAGGCCAG GGAGGAGAAGGCTAGAAAGCAGATGGAGCGGCAGCGCCTCGCTCGAGAGAAGCAGATGAGGGAGGAGGCTGAACGCACGAGGGATGAGTTGGAGAGGAGG CTGCTGCAGATGAAAGAAGAAGCAACAATGGCCAACGAAGCACTGATGCGGTCTGAGGAGACAGCTGACCTGTTGGCTGAAAAGGCCCAGATCACCGAGG AGGAGGCAAAACTTCTGGCCCAGAAGGCCGCAGAGGCTGAGCAGGAAATGCAGCGCATCAAGGCCACAGCGATTCGCACGGAGGAGGAGAAGCGCCTGAT GGAGCAGAAGGTGCTGGAAGCCGAGGTGCTGGCACTGAAGATGGCTGAGGAGTCAGAGAGGAGGGCCAAAGAGGCAGATCAGCTGAAGCAGGACCTGCAG GAAGCACGCGAGGCGGAGCGAAGAGCCAAGCAGAAGCTCCTGGAGATTGCCACCAAGCCCACGTACCCGCCCATGAACCCAATTCCAGCACCGTTGCCTC CTGACATACCAAGCTTCAACCTCATTGGTGACAGCCTGTCTTTCGACTTCAAAGATACTGACATGAAGCGGCTTTCCATGGAGATAGAGAAAGAAAAAGT GGAATACATGGAAAAGAGCAAGCATCTGCAGGAGCAGCTCAATGAACTCAAGACAGAAATCGAGGCCTTGAAACTGAAAGAGAGGGAGACAGCTCTGGAT ATTCTGCACAATGAGAACTCCGACAGGGGTGGCAGCAGCAAGCACAATACCATTAAAAAGCCTCAAGCCCAAGGCAGAAGACCTATCTGCATTTGAGCCC TCAAAGTAGGTTGTTCCCAGCTCACCTTGCAGAGCGCCAAGTCCCGAGTGGCCTTCTTTGAAGAGCTCTAGCAGGTGACCCAGCCACCCCAGGACCTGCC ACTTCTCCTGCTACCGGGACCGCGGGATGGACCAGATATCAAGAGAGCCATCCATAGGGAGCTGGCTGGGGGTTTCCGTGGGAGCTCCAGAACTTTCCCC AGCTGAGTGAAGAGCCCAGCCCCTCTTATGTGCAATTGCCTTGAACTACGACCCTGTAGAGATTTCTCTCATGGCGTTCTAGTTCTCTGACCTGAGTCTT TGTTTTAAGAAGTATTTGTCTTCCTTTGTCTAATGTGGGATTCCTGACTCCCTTCGTCCAAGGCACCGGTGTGTGTGTGTCTTGCACTCCAGAGCTGACC TCCACCGCCCAGCCTGGGAAGTCATTGTAGGGAGTGAGACACTGAAGCCCTGAGAAGCCAGTGCCATCATCCCCACCCCGCCCAGGGTTCCGGAACATTC ATTCCCCCACCGGTGAGGACCTGGCATGCAGCGAAGCAGCCCAGCCCGGCGGATCCCAGGCCAGCACGCCTGCCGGCTTCTCATCGTCAGGGAGCCCGCC CAGAGCTCGTGACGAGCAAGTGCTGGGTCCCCGCCAGGCACCCCGAGGCGGCGCTCTGGCTGGCAGCTGGTGGGGAATAGGCAGGGCAGCTGTGGCTGGG GAGAGACTTTAGGCAGAAGCTGTGATGCAGGCTGACTGCCAGCCGAGGGGCTGGGTAGTGCCGTGCGGGAGCTGATGGTACAGGGCACTCGCTGTCCCCC TCCGGCCACCCTAGACCAGGGTCCGAGAGGCAGGCAGGAGCCACTCATGTCTTCCCCATTGCCCGACGCCCATAGACGCTCCTTCCTGTGTGGGGCTGGG GTACTCCCTGGTCGTACTGCAGTCAGCACCCGTAACCCGGCTATGACCAGGGATCTGTAAGCCCTGTGGCTCCACAGGTGCTGCTTCTCACTGGCCCAGA CTCTGAGCTCCACCGGCCCAGTCTGCACGGCCCATCTGCTTCACCTTCCCTCCCAGCCACGTGCCAGTGGCCACAGCCCACTTCCCAACCCACTGTTGTA CCCAGGCCTCACTTTGCTGTTGCCCTTGTCCCTCTTCGGGCCCTGAATTTTCTGTTCCCTGGGGGCCAGCCAGGGCCCTTTGTGCCCCTCCCAGCACAGG CCTGATGCAGGTGTCCACTCACAGGTGGCGCTCACCTAGGCTGTCACAGGACCCACCTCCATGCCAGGCAACAGAGGGCCACAGAACCACCCCCACGGCT CACTCCTTGGTCTGGGGCCACCTTCTTGCCCTTTCTTTTTTTTTTTTTTTTTTTTTTTCCGAGATGGAGTCTCTCTCTGTCACCCAGGTGGGAGTGTGGT GGCACAATCTCGGCTCACTGCAACCTCCACCTCCTGAGTTCAAGCAATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGCATGCACCACCACA CTTGGCCAATGTTCTGTATTTTTAATAGGGACGGGGTTTTGCCATGTTAGCTAGGCTGGTCTCAAACTCACACCTGGGATTACAGGCATGAGCCACTGCA CCCAGCCCCTTCTTGCCCTTTCTTTTCTCCATGGCTGATGCTGCTGTGGCCAGCCAGGGCCCTTGAGATCCTTCCAGTTTGGCTGTTATGCAAAGCAGGT GATTTGTCTTAATCAGATAAAAGATAGAGGCTATGGGGGCCTCAAGATTTTTGGAGAGCAGAGGTGGTCTCTGGCAATTCCATCTGGTTTTGAGAAACTT AGCAGCTCACAGAGCACAGAGATCCTGCCTTCTTCCTACTATCAGGCTGACCTAATGGGGTTGGGCTGCTCGGCAACTGCTTGGGTCACCTTGCCCCAAG GAAACCAGCCCTGGGTGCCACCCAGCCACTTAGGGTCTACAGGGTGGGACTCCAGACCTAGAGCGTAAGTATGGATGTTGTGGCCCTGTGTCTTCCTAGT GTGACCCAGCCAGGAGCGGAAGCTTCAGGCGTTTGTAAAGTGAGGTCTGGCTCTGCCTCCTCCGTTTTTTTTTTTTTTCTGTTTCTGTTTCTGTTTTTTT TTTGAGATGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGTCACGATCTCAGCTCACTGCAACCTCCGCCTCCCAGGTACAAGAGATTCTCCTGCC TCAGCCTCCCGAGTAGCTGGGACTACAGGCGTGTGCCACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCGCCATGTTAGCCAGACTG GTCTCGAACTCCTGACCTCAGGTGATCCTCCCACCCCGGCTTCCCAAAGTTCTGGGATTATAGGCGTGAGCCACCATGCCCGGTCTCTTCTCAGTCTTGA AGCCCATCCCTGGATTTCCACCAGGAGTTACTTTCCTCCTGACCTGTAAATTTGTTCTTTAACAATGGCTGCAGGTGGGAGCATATGGTGGTTTATAAAA ACGCTGTCGGGCTTTGTTTCCTTCTTTAGCTGCCGTGTCTACTTCTGAAGTCTGGGAAGTGCCAAGCCACGCGGCCTCAAGGGAGCTGGCTGGTGTTTGA GCTGTGGCAGAAGCACCTGGGGCTCCAGGGAGCAGGCTGGGAACTGCAGGACCTTGCTCAGCCAGGAGCACTTCCCCCTCCTTGAGGCAGGAATACTGAG GTGCCTCCCCACAGATGGAGAAGGTGGAGAGGAGGATGGGCCTCAGGAGCATCTCAAGCCCCAGTAGCAGGAGAAAGAAAGAAAGAGATGCCTGGTTTTC ACAGACTGGTTCCTGTGGCTGGGATGACTGCATCCTTTTTTTTTTTTTTTTGAGACGGAGTTTTTGCCTTTGTCGCCCAGGCTGGAGTGCAATGGCGTGA TCTCGGCTCACCGCAACCTCCGCCTCCCGGATTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCACGCACCTCCACGTCCGGCT AATTTTGTATTTTTAGTGGAGACGGGGTTTCTCCATGTCGGTCAGGCTGGTCTCGAACTCCCGACCTCAGGTGATCTGCCCACCTCGGCCTCCCAAAGTG CTGGGATTACAGGCATGAGCCACCGCGCTTGGCCAGACTGCGTCCTTTTTAAGCGAACATTTTAGGGCCTGGGAGTTTGTCAAGTAAGGAAGTCTCAAGC CCAAAGAGCAGCGTCCTGACCATGGTGGTTTCATTACGAGCCCTTCTGCTGGCTCTCAGGCAGAAGCCCCACAGCACCGGGACCATTCATGAGGTCACTG CCCAGCTCATGATGTCCGTGAGGCTGTCCTTTTGGCCAGTAGCCGTGTGCAGCTGTGTGGCACAGATGGCTTCGTTCATCCTGATCAAGGCCCCACCTCA GCCACAGCAGTCCCCCCAACCTGTGTTGTCCACCCTATTATTCATGTACCTGCCAGGCCCTGCTAGATAGCACCCCGTGGCATTACATAACACTTCATGA GTGGCTGTGTCTTGTAATTTTGGGGACAGGTTTCTCTCTTTCCCTCTCTTTTTTTTGTCAAAAGCCCAGAGACTGACAACCAGCTGCAGTGTCTAAGTGT TCCTCACTGACAGGGTGGGGCCTCACCACCCCTGGAGGGAGCAGCGTTGGCAGGGAGACAGCCTGGCCCAGTGACCCTGGGCCCAAGCCAGCCCCTCCAG GGCTTTCAGGGAAGCGCCATCCATTTTCAAAGATGTCAAACGTCACTTCTTCCTGTAGGGCCCGAGTCCTGCCTCCTATCAGGGCCAGATCATAGAAGGC TATTTTCTATTCTGGGGAACGATTATAACTTAAATGATTGTTTTAATAAAAATTCTAAGCTGGAAAA

refseq_NF2.F2 (forward primer)
refseq_NF2.R2 (reverse primer)
Single exon skipping

Frame 0

GVRTVTATRARTRPMQRGPVTLVGR*EARGVWA AAV*GSRPEASPASPARIPECRVAPAPKVPAPVP SLQPSGPVPQLPFPLRQGPRGPCWPLGTRAAQT VPGPGSRPPWWP*GLCSNSRGAKGLRVQAVGRE GPGPEPRAMAGAIASRMSFSSLKRKQPKTFTVRI VTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRET WFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPV TFHFLAKFYPENAEEELVQEITQHLFFLQVKKQI LDEKIYCPPEASVLLASYAVQAKYGDYDPSVHK RGFLAQEELLPKRVINLYQMTPEMWEERITAWY AEHRGRARDEAEMEYLKIAQDLEMYGVNYFAIRN KKGTELLLGVDALGLHIYDPENRLTPKISFPWN EIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRV NKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAR EEKARKQMERQRLAREKQMREEAERTRDELERR LLQMKEEATMANEALMRSEETADLLAEKAQITE EEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLM EQKVLEAEVLALKMAEESERRAKEADQLKQDLQ EAREAERRAKQKLLEIATKPTYPPMNPIPAPLP PDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKV EYMEKSKHLQEQLNELKTEIEALKLKERETALD ILHNENSDRGGSSKHNTIKKPQAQGRRPICI*A LKVGCSQLTLQSAKSRVAFFEEL*QVTQPPQDLP LLLLPGPRDGPDIKRAIHRELAGGFRGSSRTFP S*VKSPAPLMCNCLELRPCRDFSHGVLVL*PES LF*EVFVFLCLMWDS*LPSSKAPVCVCLALQS*P PPPSLGSHCRE*DTEALRSQCHHPHPAQGSGTF IPPPVRTWHAAKQPSPADPRPARLPASHRQGAR PELVTSKCWVPARHPEAALWLAAGGE*AGQLWLG RDFRQKL*CRLTASRGAG*CRAGADGTGHSLSP SGHPRPGSERQAGATHVFPIARRP*TLLPVWGW GTPWSYCSQHP*PGYDQGSVSPVAPQVLLLTGPD SELHRPSLHGPSASPSLPATCQWPQPTSQPTVV PRPHFAVALVPLRALNFLFPGGQPGPFVPLPAQ A*CRCPLTGGAHLGCHRTHLHARQQRATEPPPRL TPWSGATFLPFLFFFFFFFPRWSLSLSPRWECG GTISAHCNLHLLSSSNSPASASQVAGTTGMHHH TWPMFCIFNRDGVLPC*LGWSQTHTWDYRHEPLH PAPSCPFFSPWLMLLWPARALEILPVWLLCKAG DLS*SDKR*RLWGPQDFWRAEVVSGNSIWF*ET *QLTEHRDPAFFLLSG*PNGVGLLGNCLGHLAPR KPALGATQPLRVYRVGLQT*SVSMDVVALCLPS VTQPGAEASGVCKVRSGSASSVFFFFLFLFLFF F*DGVSLCRPGWSAVSRSQLTATSASQVQEILLP QPPE*LGLQACATMPG*FLYF**RWGFAMLARL VSNS*PQVILPPRLPKVLGL*A*ATMPGLFSVL KPIPGFPPGVTFLLTCKFVL*QWLQVGAYGGL*K RCRALFPSLAAVSTSEVWEVPSHAASRELAGV* AVAEAPGAPGSRLGTAGPCSARSTSPSLRQEY* GASPQMEKVERRMGLRSISSPSSRRKKERDAWFS QTGSCGWDDCILFFFFLRRSFCLCRPGWSAMA* SRLTATSASRIQAILLPQPPE*LGLQARTSTSG *FCIFSGDGVSPCRSGWSRTPDLR*SAHLGLPKC WDYRHEPPRLARLRPF*ANILGPGSLSSKEVSS PKSSVLTMVVSLRALLLALRQKPHSTGTIHEVT AQLMMSVRLSFWPVAVCSCVAQMASFILIKAPPQ PQQSPQPVLSTLLFMYLPGPAR*HPVALHNTS* VAVSCNFGDRFLSFPLFFLSKAQRLTTSCSV*V FLTDRVGPHHPWREQRWQGDSLAQ*PWAQASPSR AFREAPSIFKDVKRHFFL*GPSPASYQGQIIEG YFLFWGTIIT*MIVLIKILSWK

Frame 1

E*GR*QPRARVRARCSAAP*P*SAAERRAESGP LPSRGPVPRRPRHLRPESRSAGSRLHRRSRLLC PPCSRQGPSPNSPFRSGRVLAAHAGRWGPAQPRP FPGRAAGHHGGPEACAATPGGLKGSECRPWGAR VPGLSPAPWPGPSLPA*ASALSRGSNPRRSP*G SSPWTPRWSSIAR*SGKGRTSLIWCAGLWGSEKP GSLDCSTQSRTQWPGSKWTRRYWIMMFQRKNQS PFTSWPNFILRMLKRSWFRRSHNIYSSYR*RSR F*MKRSTALLRLLCSWLLTPSRPSMVTTTPVFTS GDFWPKRNCFQKG**ICIR*LRKCGRRELLLGT QSTEAEPGMKLKWNI*R*LRTWRCTV*TTLQSG IKRAQSCCLEWMPWGFTFMTLRTD*PPRSPSRGM KSETSRTVTRSLLLNHWIRKLMSSSLTPQSFVL IS*FSSYVSGTMIYL*GEGKPILWKFSR*KPRP GRRRLESRWSGSASLERSR*GRRLNARGMSWRGG CCR*KKKQQWPTKH*CGLRRQLTCWLKRPRSPR RRQNFWPRRPQRLSRKCSASRPQRFARRRRSA* WSRRCWKPRCWH*RWLRSQRGGPKRQIS*SRTCR KHARRSEEPSRSSWRLPPSPRTRP*TQFQHRCL LTYQASTSLVTACLSTSKILT*SGFPWR*RKKK WNTWKRASICRSSSMNSRQKSRP*N*KRGRQLWI FCTMRTPTGVAAASTIPLKSLKPKAEDLSAFEP SK*VVPSSPCRAPSPEWPSLKSSSR*PSHPRTC HFSCYRDRGMDQISREPSIGSWLGVSVGAPELSP AE*RAQPLLCAIALNYDPVEISLMAF*FSDLSL CFKKYLSSFV*CGIPDSLRPRHRCVCVLHSRAD LHRPAWEVIVGSETLKP*EASAIIPTPPRVPEHS FPHR*GPGMQRSSPARRIPGQHACRLLIVREPA QSS*RASAGSPPGTPRRRSGWQLVGNRQGSCGW GETLGRSCDAG*LPAEGLGSAVRELMVQGTRCPP PATLDQGPRGRQEPLMSSPLPDAHRRSFLCGAG VLPGRTAVSTRNPAMTRDL*ALWLHRCCFSLAQ TLSSTGPVCTAHLLHLPSQPRASGHSPLPNPLLY PGLTLLLPLSLFGP*IFCSLGASQGPLCPSQHR PDAGVHSQVALT*AVTGPTSMPGNRGPQNHPHG SLLGLGPPSCPFFFFFFFFFRDGVSLCHPGGSVV AQSRLTATSTS*VQAILLPQPPK*LGLQACTTT LGQCSVFLIGTGFCHVS*AGLKLTPGITGMSHC TQPLLALSFLHG*CCCGQPGPLRSFQFGCYAKQV ICLNQIKDRGYGGLKIFGEQRWSLAIPSGFEKL SSSQSTEILPSSYYQADLMGLGCSATAWVTLPQ GNQPWVPPSHLGSTGWDSRPRA*VWMLWPCVFLV *PSQERKLQAFVK*GLALPPPFFFFFCFCFCFF FEMESRSVAQAGVQCHDLSSLQPPPPRYKRFSC LSLPSSWDYRRVPPCLANFCIFSRDGVSPC*PDW SRTPDLR*SSHPGFPKFWDYRREPPCPVSSQS* SPSLDFHQELLSS*PVNLFFNNGCRWEHMVVYK NAVGLCFLL*LPCLLLKSGKCQATRPQGSWLVFE LWQKHLGLQGAGWELQDLAQPGALPPP*GRNTE VPPHRWRRWRGGWASGASQAPVAGERKKEMPGF HRLVPVAGMTASFFFFF*DGVFAFVAQAGVQWRD LGSPQPPPPGFKQFSCLSLPSSWDYRHAPPRPA NFVFLVETGFLHVGQAGLELPTSGDLPTSASQS AGITGMSHRAWPDCVLFKRTF*GLGVCQVRKSQA QRAAS*PWWFHYEPFCWLSGRSPTAPGPFMRSL PSS*CP*GCPFGQ*PCAAVWHRWLRSS*SRPHL SHSSPPNLCCPPYYSCTCQALLDSTPWHYITLHE WLCLVILGTGFSLSLSFFCQKPRD*QPAAVSKC SSLTGWGLTTPGGSSVGRETAWPSDPGPKPAPP GLSGKRHPFSKMSNVTSSCRARVLPPIRARS*KA IFYSGERL*LK*LF**KF*AGK

Frame 2

SEDGDSHARAYAPDAARPRDPSRPLRGARSLGR CRLGVPSRGVPGISGPNPGVPGRACTEGPGSCA LPAAVRARPPTPLSAQAGSSRPMLAAGDPRSPD RSRAGQPATMVALRPVQQLQGG*RAQSAGRGARG SRA*APRHGRGHRFPHELQLSQEEATQDVHRED RHHGRRDGVQLRDEVEREGPL*FGVPDSGAPRN LVLWTAVHNQGHSGLAQNGQEGTGS*CFKGRTSH LSLLGQILS*EC*RGAGSGDHTTFILLTGKEAD FR*KDLLPS*GFCAPGFLRRPGQVW*LRPQCSQ AGIFGPRGIASKKGNKSVSDDSGNVGGENYCLVR RAPRPSQG*S*NGISEDSSGPGDVRCELLCNPE *KGHRAAAWSGCPGASHL*P*EQTDPQDLLPVE *NPKHLVQ*QGVYY*TTG*EN*CLQV*LLKASC* *ADSPAMYREP*SIYEEKESRFFGSSADESPGQ GGEG*KADGAAAPRSREADEGGG*THEG*VGEE AAADERRSNNGQRSTDAV*GDS*PVG*KGPDHRG GGKTSGPEGRRG*AGNAAHQGHSDSHGGGEAPD GAEGAGSRGAGTEDG*GVREEGQRGRSAEAGPA GSTRGGAKSQAEAPGDCHQAHVPAHEPNSSTVAS *HTKLQPHW*QPVFRLQRY*HEAAFHGDRERKS GIHGKEQASAGAAQ*TQDRNRGLETEREGDSSG YSAQ*ELRQGWQQQAQYH*KASSPRQKTYLHLSP QSRLFPAHLAERQVPSGLL*RALAGDPATPGPA TSPATGTAGWTRYQESHP*GAGWGFPWELQNFP QLSEEPSPSYVQLP*TTTL*RFLSWRSSSLT*VF VLRSICLPLSNVGFLTPFVQGTGVCVSCTPELT STAQPGKSL*GVRH*SPEKPVPSSPPRPGFRNI HSPTGEDLACSEAAQPGGSQASTPAGFSSSGSPP RARDEQVLGPRQAPRGGALAGSWWGIGRAAVAG ERL*AEAVMQADCQPRGWVVPCGS*WYRALAVP LRPP*TRVREAGRSHSCLPHCPTPIDAPSCVGLG YSLVVLQSAPVTRL*PGICKPCGSTGAASHWPR L*APPAQSARPICFTFPPSHVPVATAHFPTHCC TQASLCCCPCPSSGPEFSVPWGPARALCAPPSTG LMQVSTHRWRSPRLSQDPPPCQATEGHRTTPTA HSLVWGHLLALSFFFFFFFSEMESLSVTQVGVW WHNLGSLQPPPPEFKQFSCLSLPSSWDYRHAPPH LANVLYF**GRGFAMLARLVSNSHLGLQA*ATA PSPFLPFLFSMADAAVASQGP*DPSSLAVMQSR *FVLIR*KIEAMGASRFLESRGGLWQFHLVLRNL AAHRAQRSCLLPTIRLT*WGWAARQLLGSPCPK ETSPGCHPAT*GLQGGTPDLERKYGCCGPVSS* CDPARSGSFRRL*SEVWLCLLRFFFFSVSVSVFF LRWSLALSPRLECSVTISAHCNLRLPGTRDSPA SASRVAGTTGVCHHAWLIFVFLVEMGFRHVSQT GLELLTSGDPPTPASQSSGIIGVSHHARSLLSLE AHPWISTRSYFPPDL*ICSLTMAAGGSIWWFIK TLSGFVSFFSCRVYF*SLGSAKPRGLKGAGWCL SCGRSTWGSREQAGNCRTLLSQEHFPLLEAGILR CLPTDGEGGEEDGPQEHLKPQ*QEKERKRCLVF TDWFLWLG*LHPFFFFFETEFLPLSPRLECNGV ISAHRNLRLPDSSNSPASASRVAGITGTHLHVRL ILYF*WRRGFSMSVRLVSNSRPQVICPPRPPKV LGLQA*ATALGQTASFLSEHFRAWEFVK*GSLK PKEQRPDHGGFITSPSAGSQAEAPQHRDHS*GHC PAHDVREAVLLASSRVQLCGTDGFVHPDQGPTS ATAVPPTCVVHPIIHVPARPC*IAPRGIT*HFM SGCVL*FWGQVSLFPSLFFVKSPETDNQLQCLSV PH*QGGASPPLEGAALAGRQPGPVTLGPSQPLQ GFQGSAIHFQRCQTSLLPVGPESCLLSGPDHRR LFSILGNDYNLNDCFNKNSKLE