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Sequence NM_001030048
AGCCCCAAGCTTACCACCTGCACCCGGAGAGCTGTGTCACCATGTGGGTCCCGGTTGTCTTCCTCACCCTGTCCGTGACGTGGATTGGTGCTGCACCCCTCATCCTGTCTCGGATTGTGGGAGGCTGGGAGTGCGAGAAGCATTCCCAACCCTGGCAGGTGCTTGTGGCCTCTCGTGGCAGGGCAGTCTGCGGCGGTGTT
CTGGTGCACCCCCAGTGGGTCCTCACAGCTGCCCACTGCATCAGGAAGCCAGGTGATGACTCCAGCCACGACCTCATGCTGCTCCGCCTGTCAGAGCCTG
CCGAGCTCACGGATGCTGTGAAGGTCATGGACCTGCCCACCCAGGAGCCAGCACTGGGGACCACCTGCTACGCCTCAGGCTGGGGCAGCATTGAACCAGA
GGAGTTCTTGACCCCAAAGAAACTTCAGTGTGTGGACCTCCATGTTATTTCCAATGACGTGTGTGCGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATG
CTGTGTGCTGGACGCTGGACAGGGGGCAAAAGCACCTGCTCGGGTGATTCTGGGGGCCCACTTGTCTGTAATGGTGTGCTTCAAGGTATCACGTCATGGG
GCAGTGAACCATGTGCCCTGCCCGAAAGGCCTTCCCTGTACACCAAGGTGGTGCATTACCGGAAGTGGATCAAGGACACCATCGTGGCCAACCCCTGAGC
ACCCCTATCAACCCCCTATTGTAGTAAACTTGGAACCTTGGAAATGACCAGGCCAAGACTCAAGCCTCCCCAGTTCTACTGACCTTTGTCCTTAGGTGTG
AGGTCCAGGGTTGCTAGGAAAAGAAATCAGCAGACACAGGTGTAGACCAGAGTGTTTCTTAAATGGTGTAATTTTGTCCTCTCTGTGTCCTGGGGAATAC
TGGCCATGCCTGGAGACATATCACTCAATTTCTCTGAGGACACAGATAGGATGGGGTGTCTGTGTTATTTGTGGGGTACAGAGATGAAAGAGGGGTGGGA
TCCACACTGAGAGAGTGGAGAGTGACATGTGCTGGACACTGTCCATGAAGCACTGAGCAGAAGCTGGAGGCACAACGCACCAGACACTCACAGCAAGGAT
GGAGCTGAAAACATAACCCACTCTGTCCTGGAGGCACTGGGAAGCCTAGAGAAGGCTGTGAGCCAAGGAGGGAGGGTCTTCCTTTGGCATGGGATGGGGA
TGAAGTAAGGAGAGGGACTGGACCCCCTGGAAGCTGATTCACTATGGGGGGAGGTGTATTGAAGTCCTCCAGACAACCCTCAGATTTGATGATTTCCTAG
TAGAACTCACAGAAATAAAGAGCTGTTATACTGTG
- refseq_KLK3.F2 (forward primer)
- refseq_KLK3.R2 (reverse primer)
- Alternative acceptor
Frame 0
SPKLTTCTRRAVSPCGSRLSSSPCP*RGLVLHPSSCLGLWEAGSARSIPNPGRCLWPLVAGQSAAVF
WCTPSGSSQLPTASGSQVMTPATTSCCSACQSL
PSSRML*RSWTCPPRSQHWGPPATPQAGAALNQ
RSS*PQRNFSVWTSMLFPMTCVRKFTLRR*PSSC
CVLDAGQGAKAPARVILGAHLSVMVCFKVSRHG
AVNHVPCPKGLPCTPRWCITGSGSRTPSWPTPE
HPYQPPIVVNLEPWK*PGQDSSLPSSTDLCP*V*
GPGLLGKEISRHRCRPECFLNGVILSSLCPGEY
WPCLETYHSISLRTQIGWGVCVICGVQR*KRGG
IHTERVESDMCWTLSMKH*AEAGGTTHQTLTARM
ELKT*PTLSWRHWEA*RRL*AKEGGSSFGMGWG
*SKERDWTPWKLIHYGGRCIEVLQTTLRFDDFL
VELTEIKSCYTV
Frame 1
APSLPPAPGELCHHVGPGCLPHPVRDVDWCCTPHPVSDCGRLGVREAFPTLAGACGLSWQGSLRRC
SGAPPVGPHSCPLHQEAR**LQPRPHAAPPVRAC
RAHGCCEGHGPAHPGASTGDHLLRLRLGQH*TR
GVLDPKETSVCGPPCYFQ*RVCASSPSEGDQVH
AVCWTLDRGQKHLLG*FWGPTCL*WCASRYHVMG
Q*TMCPARKAFPVHQGGALPEVDQGHHRGQPLS
TPINPLL**TWNLGNDQAKTQASPVLLTFVLRC
EVQGC*EKKSADTGVDQSVS*MV*FCPLCVLGNT
GHAWRHITQFL*GHR*DGVSVLFVGYRDERGVG
STLREWRVTCAGHCP*STEQKLEAQRTRHSQQG
WS*KHNPLCPGGTGKPREGCEPRREGLPLAWDGD
EVRRGTGPPGS*FTMGGGVLKSSRQPSDLMIS*
*NSQK*RAVIL
Frame 2
PQAYHLHPESCVTMWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGV
LVHPQWVLTAAHCIRKPGDDSSHDLMLLRLSEP
AELTDAVKVMDLPTQEPALGTTCYASGWGSIEPE
EFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFM
LCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSW
GSEPCALPERPSLYTKVVHYRKWIKDTIVANP*A
PLSTPYCSKLGTLEMTRPRLKPPQFY*PLSLGV
RSRVARKRNQQTQV*TRVFLKWCNFVLSVSWGI
LAMPGDISLNFSEDTDRMGCLCYLWGTEMKEGWD
PH*ESGE*HVLDTVHEALSRSWRHNAPDTHSKD
GAENITHSVLEALGSLEKAVSQGGRVFLWHGMG
MK*GEGLDPLEADSLWGEVY*SPPDNPQI**FPS
RTHRNKELLYC
Sequence NM_001030047
AGCCCCAAGCTTACCACCTGCACCCGGAGAGCTGTGTCACCATGTGGGTCCCGGTTGTCTTCCTCACCCTGTCCGTGACGTGGATTGGTGCTGCACCCCTCATCCTGTCTCGGATTGTGGGAGGCTGGGAGTGCGAGAAGCATTCCCAACCCTGGCAGGTGCTTGTGGCCTCTCGTGGCAGGGCAGTCTGCGGCGGTGTT
CTGGTGCACCCCCAGTGGGTCCTCACAGCTGCCCACTGCATCAGGAACAAAAGCGTGATCTTGCTGGGTCGGCACAGCCTGTTTCATCCTGAAGACACAG
GCCAGGTATTTCAGGTCAGCCACAGCTTCCCACACCCGCTCTACGATATGAGCCTCCTGAAGAATCGATTCCTCAGGCCAGGTGATGACTCCAGCCACGA
CCTCATGCTGCTCCGCCTGTCAGAGCCTGCCGAGCTCACGGATGCTGTGAAGGTCATGGACCTGCCCACCCAGGAGCCAGCACTGGGGACCACCTGCTAC
GCCTCAGGCTGGGGCAGCATTGAACCAGAGGAGTTCTTGACCCCAAAGAAACTTCAGTGTGTGGACCTCCATGTTATTTCCAATGACGTGTGTGCGCAAG
TTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGCTGGACAGGGGGCAAAAGCACCTGCTCGTGGGTCATTCTGATCACCGAACTGACCAT
GCCAGCCCTGCCGATGGTCCTCCATGGCTCCCTAGTGCCCTGGAGAGGAGGTGTCTAGTCAGAGAGTAGTCCTGGAAGGTGGCCTCTGTGAGGAGCCACG
GGGACAGCATCCTGCAGATGGTCCTGGCCCTTGTCCCACCGACCTGTCTACAAGGACTGTCCTCGTGGACCCTCCCCTCTGCACAGGAGCTGGACCCTGA
AGTCCCTTCCCCACCGGCCAGGACTGGAGCCCCTACCCCTCTGTTGGAATCCCTGCCCACCTTCTTCTGGAAGTCGGCTCTGGAGACATTTCTCTCTTCT
TCCAAAGCTGGGAACTGCTATCTGTTATCTGCCTGTCCAGGTCTGAAAGATAGGATTGCCCAGGCAGAAACTGGGACTGACCTATCTCACTCTCTCCCTG
CTTTTACCCTTAGGGTGATTCTGGGGGCCCACTTGTCTGTAATGGTGTGCTTCAAGGTATCACGTCATGGGGCAGTGAACCATGTGCCCTGCCCGAAAGG
CCTTCCCTGTACACCAAGGTGGTGCATTACCGGAAGTGGATCAAGGACACCATCGTGGCCAACCCCTGAGCACCCCTATCAACCCCCTATTGTAGTAAAC
TTGGAACCTTGGAAATGACCAGGCCAAGACTCAAGCCTCCCCAGTTCTACTGACCTTTGTCCTTAGGTGTGAGGTCCAGGGTTGCTAGGAAAAGAAATCA
GCAGACACAGGTGTAGACCAGAGTGTTTCTTAAATGGTGTAATTTTGTCCTCTCTGTGTCCTGGGGAATACTGGCCATGCCTGGAGACATATCACTCAAT
TTCTCTGAGGACACAGATAGGATGGGGTGTCTGTGTTATTTGTGGGGTACAGAGATGAAAGAGGGGTGGGATCCACACTGAGAGAGTGGAGAGTGACATG
TGCTGGACACTGTCCATGAAGCACTGAGCAGAAGCTGGAGGCACAACGCACCAGACACTCACAGCAAGGATGGAGCTGAAAACATAACCCACTCTGTCCT
GGAGGCACTGGGAAGCCTAGAGAAGGCTGTGAGCCAAGGAGGGAGGGTCTTCCTTTGGCATGGGATGGGGATGAAGTAAGGAGAGGGACTGGACCCCCTG
GAAGCTGATTCACTATGGGGGGAGGTGTATTGAAGTCCTCCAGACAACCCTCAGATTTGATGATTTCCTAGTAGAACTCACAGAAATAAAGAGCTGTTAT
ACTGTG
- refseq_KLK3.F2 (forward primer)
- refseq_KLK3.R2 (reverse primer)
- Alternative acceptor
Frame 0
SPKLTTCTRRAVSPCGSRLSSSPCP*RGLVLHPSSCLGLWEAGSARSIPNPGRCLWPLVAGQSAAVF
WCTPSGSSQLPTASGTKA*SCWVGTACFILKTQ
ARYFRSATASHTRSTI*AS*RIDSSGQVMTPAT
TSCCSACQSLPSSRML*RSWTCPPRSQHWGPPAT
PQAGAALNQRSS*PQRNFSVWTSMLFPMTCVRK
FTLRR*PSSCCVLDAGQGAKAPARGSF*SPN*P
CQPCRWSSMAP*CPGEEVSSQRVVLEGGLCEEPR
GQHPADGPGPCPTDLSTRTVLVDPPLCTGAGP*
SPFPTGQDWSPYPSVGIPAHLLLEVGSGDISLF
FQSWELLSVICLSRSER*DCPGRNWD*PISLSPC
FYP*GDSGGPLVCNGVLQGITSWGSEPCALPER
PSLYTKVVHYRKWIKDTIVANP*APLSTPYCSK
LGTLEMTRPRLKPPQFY*PLSLGVRSRVARKRNQ
QTQV*TRVFLKWCNFVLSVSWGILAMPGDISLN
FSEDTDRMGCLCYLWGTEMKEGWDPH*ESGE*H
VLDTVHEALSRSWRHNAPDTHSKDGAENITHSVL
EALGSLEKAVSQGGRVFLWHGMGMK*GEGLDPL
EADSLWGEVY*SPPDNPQI**FPSRTHRNKELL
YC
Frame 1
APSLPPAPGELCHHVGPGCLPHPVRDVDWCCTPHPVSDCGRLGVREAFPTLAGACGLSWQGSLRRC
SGAPPVGPHSCPLHQEQKRDLAGSAQPVSS*RHR
PGISGQPQLPTPALRYEPPEESIPQAR**LQPR
PHAAPPVRACRAHGCCEGHGPAHPGASTGDHLL
RLRLGQH*TRGVLDPKETSVCGPPCYFQ*RVCAS
SPSEGDQVHAVCWTLDRGQKHLLVGHSDHRTDH
ASPADGPPWLPSALERRCLVRE*SWKVASVRSH
GDSILQMVLALVPPTCLQGLSSWTLPSAQELDPE
VPSPPARTGAPTPLLESLPTFFWKSALETFLSS
SKAGNCYLLSACPGLKDRIAQAETGTDLSHSLP
AFTLRVILGAHLSVMVCFKVSRHGAVNHVPCPKG
LPCTPRWCITGSGSRTPSWPTPEHPYQPPIVVN
LEPWK*PGQDSSLPSSTDLCP*V*GPGLLGKEI
SRHRCRPECFLNGVILSSLCPGEYWPCLETYHSI
SLRTQIGWGVCVICGVQR*KRGGIHTERVESDM
CWTLSMKH*AEAGGTTHQTLTARMELKT*PTLS
WRHWEA*RRL*AKEGGSSFGMGWG*SKERDWTPW
KLIHYGGRCIEVLQTTLRFDDFLVELTEIKSCY
TV
Frame 2
PQAYHLHPESCVTMWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGV
LVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDT
GQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHD
LMLLRLSEPAELTDAVKVMDLPTQEPALGTTCY
ASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQ
VHPQKVTKFMLCAGRWTGGKSTCSWVILITELTM
PALPMVLHGSLVPWRGGV*SESSPGRWPL*GAT
GTASCRWSWPLSHRPVYKDCPRGPSPLHRSWTL
KSLPHRPGLEPLPLCWNPCPPSSGSRLWRHFSLL
PKLGTAICYLPVQV*KIGLPRQKLGLTYLTLSL
LLPLG*FWGPTCL*WCASRYHVMGQ*TMCPARK
AFPVHQGGALPEVDQGHHRGQPLSTPINPLL**T
WNLGNDQAKTQASPVLLTFVLRCEVQGC*EKKS
ADTGVDQSVS*MV*FCPLCVLGNTGHAWRHITQ
FL*GHR*DGVSVLFVGYRDERGVGSTLREWRVTC
AGHCP*STEQKLEAQRTRHSQQGWS*KHNPLCP
GGTGKPREGCEPRREGLPLAWDGDEVRRGTGPP
GS*FTMGGGVLKSSRQPSDLMIS**NSQK*RAVI
L
Sequence NM_001648
AGCCCCAAGCTTACCACCTGCACCCGGAGAGCTGTGTCACCATGTGGGTCCCGGTTGTCTTCCTCACCCTGTCCGTGACGTGGATTGGTGCTGCACCCCTCATCCTGTCTCGGATTGTGGGAGGCTGGGAGTGCGAGAAGCATTCCCAACCCTGGCAGGTGCTTGTGGCCTCTCGTGGCAGGGCAGTCTGCGGCGGTGTT
CTGGTGCACCCCCAGTGGGTCCTCACAGCTGCCCACTGCATCAGGAACAAAAGCGTGATCTTGCTGGGTCGGCACAGCCTGTTTCATCCTGAAGACACAG
GCCAGGTATTTCAGGTCAGCCACAGCTTCCCACACCCGCTCTACGATATGAGCCTCCTGAAGAATCGATTCCTCAGGCCAGGTGATGACTCCAGCCACGA
CCTCATGCTGCTCCGCCTGTCAGAGCCTGCCGAGCTCACGGATGCTGTGAAGGTCATGGACCTGCCCACCCAGGAGCCAGCACTGGGGACCACCTGCTAC
GCCTCAGGCTGGGGCAGCATTGAACCAGAGGAGTTCTTGACCCCAAAGAAACTTCAGTGTGTGGACCTCCATGTTATTTCCAATGACGTGTGTGCGCAAG
TTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGCTGGACAGGGGGCAAAAGCACCTGCTCGGGTGATTCTGGGGGCCCACTTGTCTGTAA
TGGTGTGCTTCAAGGTATCACGTCATGGGGCAGTGAACCATGTGCCCTGCCCGAAAGGCCTTCCCTGTACACCAAGGTGGTGCATTACCGGAAGTGGATC
AAGGACACCATCGTGGCCAACCCCTGAGCACCCCTATCAACCCCCTATTGTAGTAAACTTGGAACCTTGGAAATGACCAGGCCAAGACTCAAGCCTCCCC
AGTTCTACTGACCTTTGTCCTTAGGTGTGAGGTCCAGGGTTGCTAGGAAAAGAAATCAGCAGACACAGGTGTAGACCAGAGTGTTTCTTAAATGGTGTAA
TTTTGTCCTCTCTGTGTCCTGGGGAATACTGGCCATGCCTGGAGACATATCACTCAATTTCTCTGAGGACACAGATAGGATGGGGTGTCTGTGTTATTTG
TGGGGTACAGAGATGAAAGAGGGGTGGGATCCACACTGAGAGAGTGGAGAGTGACATGTGCTGGACACTGTCCATGAAGCACTGAGCAGAAGCTGGAGGC
ACAACGCACCAGACACTCACAGCAAGGATGGAGCTGAAAACATAACCCACTCTGTCCTGGAGGCACTGGGAAGCCTAGAGAAGGCTGTGAGCCAAGGAGG
GAGGGTCTTCCTTTGGCATGGGATGGGGATGAAGTAAGGAGAGGGACTGGACCCCCTGGAAGCTGATTCACTATGGGGGGAGGTGTATTGAAGTCCTCCA
GACAACCCTCAGATTTGATGATTTCCTAGTAGAACTCACAGAAATAAAGAGCTGTTATACTGTG
- refseq_KLK3.F2 (forward primer)
- refseq_KLK3.R2 (reverse primer)
- Alternative acceptor
Frame 0
SPKLTTCTRRAVSPCGSRLSSSPCP*RGLVLHPSSCLGLWEAGSARSIPNPGRCLWPLVAGQSAAVF
WCTPSGSSQLPTASGTKA*SCWVGTACFILKTQ
ARYFRSATASHTRSTI*AS*RIDSSGQVMTPAT
TSCCSACQSLPSSRML*RSWTCPPRSQHWGPPAT
PQAGAALNQRSS*PQRNFSVWTSMLFPMTCVRK
FTLRR*PSSCCVLDAGQGAKAPARVILGAHLSV
MVCFKVSRHGAVNHVPCPKGLPCTPRWCITGSGS
RTPSWPTPEHPYQPPIVVNLEPWK*PGQDSSLP
SSTDLCP*V*GPGLLGKEISRHRCRPECFLNGV
ILSSLCPGEYWPCLETYHSISLRTQIGWGVCVIC
GVQR*KRGGIHTERVESDMCWTLSMKH*AEAGG
TTHQTLTARMELKT*PTLSWRHWEA*RRL*AKE
GGSSFGMGWG*SKERDWTPWKLIHYGGRCIEVLQ
TTLRFDDFLVELTEIKSCYTV
Frame 1
APSLPPAPGELCHHVGPGCLPHPVRDVDWCCTPHPVSDCGRLGVREAFPTLAGACGLSWQGSLRRC
SGAPPVGPHSCPLHQEQKRDLAGSAQPVSS*RHR
PGISGQPQLPTPALRYEPPEESIPQAR**LQPR
PHAAPPVRACRAHGCCEGHGPAHPGASTGDHLL
RLRLGQH*TRGVLDPKETSVCGPPCYFQ*RVCAS
SPSEGDQVHAVCWTLDRGQKHLLG*FWGPTCL*
WCASRYHVMGQ*TMCPARKAFPVHQGGALPEVD
QGHHRGQPLSTPINPLL**TWNLGNDQAKTQASP
VLLTFVLRCEVQGC*EKKSADTGVDQSVS*MV*
FCPLCVLGNTGHAWRHITQFL*GHR*DGVSVLF
VGYRDERGVGSTLREWRVTCAGHCP*STEQKLEA
QRTRHSQQGWS*KHNPLCPGGTGKPREGCEPRR
EGLPLAWDGDEVRRGTGPPGS*FTMGGGVLKSS
RQPSDLMIS**NSQK*RAVIL
Frame 2
PQAYHLHPESCVTMWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGV
LVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDT
GQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHD
LMLLRLSEPAELTDAVKVMDLPTQEPALGTTCY
ASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQ
VHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCN
GVLQGITSWGSEPCALPERPSLYTKVVHYRKWI
KDTIVANP*APLSTPYCSKLGTLEMTRPRLKPP
QFY*PLSLGVRSRVARKRNQQTQV*TRVFLKWCN
FVLSVSWGILAMPGDISLNFSEDTDRMGCLCYL
WGTEMKEGWDPH*ESGE*HVLDTVHEALSRSWR
HNAPDTHSKDGAENITHSVLEALGSLEKAVSQGG
RVFLWHGMGMK*GEGLDPLEADSLWGEVY*SPP
DNPQI**FPSRTHRNKELLYC
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