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Sequences

Sequence NM_145887

GCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTGCGTGCTGGGACATGTCTGGCCTCCAAGGACCGTCGGTGGGCGATGGCTGCAACGGTGGAGGGGCCA
GAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTTCAGAGGATTCGGACGCAGGGTCCAGGGCGCTGCCTTTCCTGGGCGGCAACCGGCTGAGCTTGGACC
TGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCTGTGTGTCCAGCAGCCTCTGCAGCTGCTGCAGGTGGAATTCTTGCGTCTGAGCACTCACGAGGACCC
TCAGCTGCTGGAGGCCACCCTGGCCCAGCTGCCTCAGAGCCTGTCCTGCCTCCGCTCCCTGGTCCTCAAAGGAGGGCAACGCCGGGACACACTGGGTGCC
TGTCTCCGGGGTGCCCTGACCAACCTGCCCGCTGGTCTGAGTGGCCTGGCCCATCTGGCCCACCTGGACCTGAGCTTCAACAGCCTGGAGACACTGCCGG
CCTGTGTCCTGCAGATGCGAGGTCTGGGTGCGCTCTTGCTGTCTCACAACTGCCTCTCTGAGCTGCCTGAGGCTCTGGGGGCCCTCCCCGCCCTCACCTT
CCTCACAGTGACACACAACCGCCTGCAGACGCTGCCCCCAGCACTGGGGGCCCTATCCACCCTGCAGCGCCTCGATCTCTCTCAGAATCTGCTGGACACG
CTACCTCCTGAGATTGGAGGCCTGGGCAGCCTCCTGGAGCTCAACCTGGCCTCCAACCGGCTGCAGAGCCTCCCAGCCTCTCTGGCGGGACTTCGGTCCT
TGCGGCTCCTTGTCCTGCACAGCAACCTCCTGGCCTCTGTGCCAGCTGACTTGGCCCGCCTTCCACTCCTCACCCGGCTCGACCTGAGGGACAACCAGCT
CCGGGACCTGCCCCCTGAGCTGCTAGACGCCCCCTTTGTGCGCCTGCAGGGGAACCCCCTGGGTGAGGCCTCGCCAGACGCCCCGAGTTCACCAGTGGCA
GCCCTCATTCCAGAAATGCCCAGACTGTTCCTGACCTCAGATTTGGACAGCTTTCCTGTGACCCCTCAAGGCTGCTCAGTGACCCTGGCCTGTGGCGTCC
GCCTGCAGTTCCCAGCGGGAGCCACCGCCACCCCCATCACCATCCGCTATCGGCTGCTGCTGCCGGAGCCAGGCCTCGTCCCCCTGGGTCCTCATGACGC
CCTGCTCAGCCATGTGCTGGAGCTGCAGCCCCATGGGGTGGCCTTCCAGCAGGATGTGGGGCTGTGGCTGCTCTTCACCCCACCGCAGGCCCGGCGCTGC
CGTGAAGTGGTGGTCAGGACCCGGAATGACAACAGCTGGGGTGACCTGGAGACCTACCTGGAGGAAGAGGCACCCCAGCGGCTCTGGGCTCACTGCCAGG
TGCCCCACTTCTCCTGGTTCCTTGTGGTTTCCCGCCCTGTGTCCAATGCCTGCCTGGTGCCACCGGAGGGGACACTGCTGTGCTCCTCGGGTCATCCTGG
GGTCAAAGTCATCTTCCCCCCTGGGGCCACTGAGGAGCCTCGTCGAGTCTCCATGCAGGTGGTGCGCATGGCTGGCCGAGAGCTGCAGGCCCTCCTGGGA
GAACCAGAGGCTGCAGTGAGCCCCCTGCTGTGCCTGTCACAGAGCGGTCCCCCCAGCTTCCTCCAACCGGTCACCGTGCAGCTGCCTCTGCCCTCTGGCA
TCACAGGCCTCAGTCTGGACCGCTCCCGCCTGCACCTGTTGTACTGGGCCCCTCCTGCAGCCACCTGGGATGACATCACAGCTCAGGTGGTCCTGGAGCT
CACCCACCTGTACGCACGCTTCCAGGTCACACACTTCTCCTGGTACTGGCTCTGGTACACCACCAAGAACTGTGTGGGAGGCCTGGCTCGGAAGGCCTGG
GAGCGGCTGCGGCTGCACCGTGTGAACCTCATCGCTCTGCAGCGGCGCCGGGACCCTGAGCAGGTCCTGCTGCAGTGCCTGCCCCGAAACAAGGTGGACG
CCACCCTTCGGCGGCTGCTGGAGCGGTACCGGGGCCCCGAGCCCTCTGACACGGTGGAGATGTTCGAGGGCGAAGAGTTCTTTGCGGCCTTCGAGCGCGG
CATCGACGTGGATGCTGACCGCCCTGACTGTGTGGAGGGCAGAATCTGCTTTGTCTTCTACTCGCACCTGAAGAATGTGAAGGAGGTGTCCTTCTACCGT
GGCG
CGGTGCCTGTGCGGGTGCCCGAGGAGGCTGAGGCTGCCCGGCAGAGGAAGGGCGCAGACGCCCTGTGGATGGCCACTCTGCCCATCAAGCTGCCGA
GACTTCGAGGGTCCGAGGGGCCACGGCGGGGGGCTGGCCTCTCCTTGGCACCCTTGAATCTGGGAGATGCCGAGACCGGCTTTCTGACGCAGAGCAACCT
GCTGAGTGTGGCTGGGCGTCTGGGTCTGGACTGGCCAGCCGTGGCCCTGCACCTGGGGGTGTCCTACCGGGAGGTGCAGCGCATCCGGCACGAGTTCCGG
GATGATCTGGATGAGCAGATCCGTCACATGCTCTTCTCCTGGGCTGAGCGCCAGGCTGGGCAGCCAGGGGCTGTGGGGCTCCTGGTGCAGGCCCTGGAGC
AGAGTGACCGGCAGGACGTGGCTGAAGAGGTGCGCGCAGTCTTGGAGCTCGGCCGCCGCAAGTACCAGGACAGCATCCGACGCATGGGCTTGGCCCCCAA
GGACCCCGCTCTGCCTGGCTCCTCGGCTCCACAGCCCCCAGAGCCTGCCCAGGCCTAGGCCCCACAGACTTTTAGGCTGGCCCAGATATTCCCCAGTGGA
TGGGCAGAGCCCCCACCTTCAAGTCTCTCCAGTGTGTGGGGACGGGTCCCTGTGAGCAACAAAACTGCACTGTTTCTTTCACCTCA
  • refseq_LRDD.F6 (forward primer)
  • refseq_LRDD.R6 (reverse primer)
  • Alternative donor

Frame 0

ASPGAAWTGLPACWDMSGLQGPSVGDGCNGGGA
RAGGSCCRRRCFRGFGRRVQGAAFPGRQPAELGP
VPRGLPAAAAPVCPAASAAAAGGILASEHSRGP
SAAGGHPGPAASEPVLPPLPGPQRRATPGHTGC
LSPGCPDQPARWSEWPGPSGPPGPELQQPGDTAG
LCPADARSGCALAVSQLPL*AA*GSGGPPRPHL
PHSDTQPPADAAPSTGGPIHPAAPRSLSESAGH
ATS*DWRPGQPPGAQPGLQPAAEPPSLSGGTSVL
AAPCPAQQPPGLCAS*LGPPSTPHPARPEGQPA
PGPAP*AARRPLCAPAGEPPG*GLARRPEFTSG
SPHSRNAQTVPDLRFGQLSCDPSRLLSDPGLWRP
PAVPSGSHRHPHHHPLSAAAAGARPRPPGSS*R
PAQPCAGAAAPWGGLPAGCGAVAALHPTAGPAL
P*SGGQDPE*QQLG*PGDLPGGRGTPAALGSLPG
APLLLVPCGFPPCVQCLPGATGGDTAVLLGSSW
GQSHLPPWGH*GASSSLHAGGAHGWPRAAGPPG
RTRGCSEPPAVPVTERSPQLPPTGHRAAASALWH
HRPQSGPLPPAPVVLGPSCSHLG*HHSSGGPGA
HPPVRTLPGHTLLLVLALVHHQELCGRPGSEGL
GAAAAAPCEPHRSAAAPGP*AGPAAVPAPKQGGR
HPSAAAGAVPGPRAL*HGGDVRGRRVLCGLRAR
HRRGC*PP*LCGGQNLLCLLLAPEECEGGVLLP
WR
GACAGARGG*GCPAEEGRRRPVDGHSAHQAAE
TSRVRGATAGGWPLLGTLESGRCRDRLSDAEQP
AECGWASGSGLASRGPAPGGVLPGGAAHPARVP
G*SG*ADPSHALLLG*APGWAARGCGAPGAGPGA
E*PAGRG*RGARSLGARPPQVPGQHPTHGLGPQ
GPRSAWLLGSTAPRACPGLGPTDF*AGPDIPQW
MGRAPTFKSLQCVGTGPCEQQNCTVSFTS

Frame 1

LPRALPGQACLRAGTCLASKDRRWAMAATVEGP
ELEAAAAAGDASEDSDAGSRALPFLGGNRLSLD
LYPGGCQQLLHLCVQQPLQLLQVEFLRLSTHEDP
QLLEATLAQLPQSLSCLRSLVLKGGQRRDTLGA
CLRGALTNLPAGLSGLAHLAHLDLSFNSLETLP
ACVLQMRGLGALLLSHNCLSELPEALGALPALTF
LTVTHNRLQTLPPALGALSTLQRLDLSQNLLDT
LPPEIGGLGSLLELNLASNRLQSLPASLAGLRS
LRLLVLHSNLLASVPADLARLPLLTRLDLRDNQL
RDLPPELLDAPFVRLQGNPLGEASPDAPSSPVA
ALIPEMPRLFLTSDLDSFPVTPQGCSVTLACGV
RLQFPAGATATPITIRYRLLLPEPGLVPLGPHDA
LLSHVLELQPHGVAFQQDVGLWLLFTPPQARRC
REVVVRTRNDNSWGDLETYLEEEAPQRLWAHCQ
VPHFSWFLVVSRPVSNACLVPPEGTLLCSSGHPG
VKVIFPPGATEEPRRVSMQVVRMAGRELQALLG
EPEAAVSPLLCLSQSGPPSFLQPVTVQLPLPSG
ITGLSLDRSRLHLLYWAPPAATWDDITAQVVLEL
THLYARFQVTHFSWYWLWYTTKNCVGGLARKAW
ERLRLHRVNLIALQRRRDPEQVLLQCLPRNKVD
ATLRRLLERYRGPEPSDTVEMFEGEEFFAAFERG
IDVDADRPDCVEGRICFVFYSHLKNVKEVSFYR
G
AVPVRVPEEAEAARQRKGADALWMATLPIKLP
RLRGSEGPRRGAGLSLAPLNLGDAETGFLTQSNL
LSVAGRLGLDWPAVALHLGVSYREVQRIRHEFR
DDLDEQIRHMLFSWAERQAGQPGAVGLLVQALE
QSDRQDVAEEVRAVLELGRRKYQDSIRRMGLAPK
DPALPGSSAPQPPEPAQA*APQTFRLAQIFPSG
WAEPPPSSLSSVWGRVPVSNKTALFLSP

Frame 2

FPGRCLDRPACVLGHVWPPRTVGGRWLQRWRGQ
SWRQLLPQEMLQRIRTQGPGRCLSWAATG*AWT
CTPGAASSCCTCVSSSLCSCCRWNSCV*ALTRT
LSCWRPPWPSCLRACPASAPWSSKEGNAGTHWVP
VSGVP*PTCPLV*VAWPIWPTWT*ASTAWRHCR
PVSCRCEVWVRSCCLTTASLSCLRLWGPSPPSP
SSQ*HTTACRRCPQHWGPYPPCSASISLRICWTR
YLLRLEAWAASWSSTWPPTGCRASQPLWRDFGP
CGSLSCTATSWPLCQLTWPAFHSSPGST*GTTS
SGTCPLSC*TPPLCACRGTPWVRPRQTPRVHQWQ
PSFQKCPDCS*PQIWTAFL*PLKAAQ*PWPVAS
ACSSQREPPPPPSPSAIGCCCRSQASSPWVLMT
PCSAMCWSCSPMGWPSSRMWGCGCSSPHRRPGAA
VKWWSGPGMTTAGVTWRPTWRKRHPSGSGLTAR
CPTSPGSLWFPALCPMPAWCHRRGHCCAPRVIL
GSKSSSPLGPLRSLVESPCRWCAWLAESCRPSWE
NQRLQ*APCCACHRAVPPASSNRSPCSCLCPLA
SQASVWTAPACTCCTGPLLQPPGMTSQLRWSWS
SPTCTHASRSHTSPGTGSGTPPRTVWEAWLGRPG
SGCGCTV*TSSLCSGAGTLSRSCCSACPETRWT
PPFGGCWSGTGAPSPLTRWRCSRAKSSLRPSSA
ASTWMLTALTVWRAESALSSTRT*RM*RRCPSTV
A
RCLCGCPRRLRLPGRGRAQTPCGWPLCPSSCR
DFEGPRGHGGGLASPWHP*IWEMPRPAF*RRAT
C*VWLGVWVWTGQPWPCTWGCPTGRCSASGTSSG
MIWMSRSVTCSSPGLSARLGSQGLWGSWCRPWS
RVTGRTWLKRCAQSWSSAAASTRTASDAWAWPP
RTPLCLAPRLHSPQSLPRPRPHRLLGWPRYSPVD
GQSPHLQVSPVCGDGSL*ATKLHCFFHL

Sequence NM_145886

GCCGGCGGGATCCCAGGCGCCGAGCGCCCGCTGAGCAGCCACCCTTTGCGCGCCGCCTGCAGCGCAGCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTG
CGTGCTGGGACATGTCTGGCCTCCAAGGACCGTCGGTGGGCGATGGCTGCAACGGTGGAGGGGCCAGAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTT
CAGAGGATTCGGACGCAGGGTCCAGGGCGCTGCCTTTCCTGGGCGGCAACCGGCTGAGCTTGGACCTGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCT
GTGTGTCCAGCAGCCTCTGCAGCTGCTGCAGGTGGAATTCTTGCGTCTGAGCACTCACGAGGACCCTCAGCTGCTGGAGGCCACCCTGGCCCAGCTGCCT
CAGAGCCTGTCCTGCCTCCGCTCCCTGGTCCTCAAAGGAGGGCAACGCCGGGACACACTGGGTGCCTGTCTCCGGGGTGCCCTGACCAACCTGCCCGCTG
GTCTGAGTGGCCTGGCCCATCTGGCCCACCTGGACCTGAGCTTCAACAGCCTGGAGACACTGCCGGCCTGTGTCCTGCAGATGCGAGGTCTGGGTGCGCT
CTTGCTGTCTCACAACTGCCTCTCTGAGCTGCCTGAGGCTCTGGGGGCCCTCCCCGCCCTCACCTTCCTCACAGTGACACACAACCGCCTGCAGACGCTG
CCCCCAGCACTGGGGGCCCTATCCACCCTGCAGCGCCTCGATCTCTCTCAGAATCTGCTGGACACGCTACCTCCTGAGATTGGAGGCCTGGGCAGCCTCC
TGGAGCTCAACCTGGCCTCCAACCGGCTGCAGAGCCTCCCAGCCTCTCTGGCGGGACTTCGGTCCTTGCGGCTCCTTGTCCTGCACAGCAACCTCCTGGC
CTCTGTGCCAGCTGACTTGGCCCGCCTTCCACTCCTCACCCGGCTCGACCTGAGGGACAACCAGCTCCGGGACCTGCCCCCTGAGCTGCTAGACGCCCCC
TTTGTGCGCCTGCAGGGGAACCCCCTGGGTGAGGCCTCGCCAGACGCCCCGAGTTCACCAGTGGCAGCCCTCATTCCAGAAATGCCCAGACTGTTCCTGA
CCTCAGATTTGGACAGCTTTCCTGTGACCCCTCAAGGCTGCTCAGTGACCCTGGCCTGTGGCGTCCGCCTGCAGTTCCCAGCGGGAGCCACCGCCACCCC
CATCACCATCCGCTATCGGCTGCTGCTGCCGGAGCCAGGCCTCGTCCCCCTGGGTCCTCATGACGCCCTGCTCAGCCATGTGCTGGAGCTGCAGCCCCAT
GGGGTGGCCTTCCAGCAGGATGTGGGGCTGTGGCTGCTCTTCACCCCACCGCAGGCCCGGCGCTGCCGTGAAGTGGTGGTCAGGACCCGGAATGACAACA
GCTGGGGTGACCTGGAGACCTACCTGGAGGAAGAGGCACCCCAGCGGCTCTGGGCTCACTGCCAGGTGCCCCACTTCTCCTGGTTCCTTGTGGTTTCCCG
CCCTGTGTCCAATGCCTGCCTGGTGCCACCGGAGGGGACACTGCTGTGCTCCTCGGGTCATCCTGGGGTCAAAGTCATCTTCCCCCCTGGGGCCACTGAG
GAGCCTCGTCGAGTCTCCATGCAGGTGGTGCGCATGGCTGGCCGAGAGCTGCAGGCCCTCCTGGGAGAACCAGAGGCTGCAGTGAGCCCCCTGCTGTGCC
TGTCACAGAGCGGTCCCCCCAGCTTCCTCCAACCGGTCACCGTGCAGCTGCCTCTGCCCTCTGGCATCACAGGCCTCAGTCTGGACCGCTCCCGCCTGCA
CCTGTTGTACTGGGCCCCTCCTGCAGCCACCTGGGATGACATCACAGCTCAGGTGGTCCTGGAGCTCACCCACCTGTACGCACGCTTCCAGGTCACACAC
TTCTCCTGGTACTGGCTCTGGTACACCACCAAGAACTGTGTGGGAGGCCTGGCTCGGAAGGCCTGGGAGCGGCTGCGGCTGCACCGTGTGAACCTCATCG
CTCTGCAGCGGCGCCGGGACCCTGAGCAGGTCCTGCTGCAGTGCCTGCCCCGAAACAAGGTGGACGCCACCCTTCGGCGGCTGCTGGAGCGGTACCGGGG
CCCCGAGCCCTCTGACACGGTGGAGATGTTCGAGGGCGAAGAGTTCTTTGCGGCCTTCGAGCGCGGCATCGACGTGGATGCTGACCGCCCTGACTGTGTG
GAGGGCAGAATCTGCTTTGTCTTCTACTCGCACCTGAAGAATGTGAAGGAGGTATACGTGACCACCACTCTGGACCGGGAGGCTCAGGCTGTGCGGGGCC
AG
GTGTCCTTCTACCGTGGCGCGGTGCCTGTGCGGGTGCCCGAGGAGGCTGAGGCTGCCCGGCAGAGGAAGGGCGCAGACGCCCTGTGGATGGCCACTCT
GCCCATCAAGCTGCCGAGACTTCGAGGGTCCGAGGGGCCACGGCGGGGGGCTGGCCTCTCCTTGGCACCCTTGAATCTGGGAGATGCCGAGACCGGCTTT
CTGACGCAGAGCAACCTGCTGAGTGTGGCTGGGCGTCTGGGTCTGGACTGGCCAGCCGTGGCCCTGCACCTGGGGGTGTCCTACCGGGAGGTGCAGCGCA
TCCGGCACGAGTTCCGGGATGATCTGGATGAGCAGATCCGTCACATGCTCTTCTCCTGGGCTGAGCGCCAGGCTGGGCAGCCAGGGGCTGTGGGGCTCCT
GGTGCAGGCCCTGGAGCAGAGTGACCGGCAGGACGTGGCTGAAGAGGTGCGCGCAGTCTTGGAGCTCGGCCGCCGCAAGTACCAGGACAGCATCCGACGC
ATGGGCTTGGCCCCCAAGGACCCCGCTCTGCCTGGCTCCTCGGCTCCACAGCCCCCAGAGCCTGCCCAGGCCTAGGCCCCACAGACTTTTAGGCTGGCCC
AGATATTCCCCAGTGGATGGGCAGAGCCCCCACCTTCAAGTCTCTCCAGTGTGTGGGGACGGGTCCCTGTGAGCAACAAAACTGCACTGTTTCTTTCACC
TCA
  • refseq_LRDD.F6 (forward primer)
  • refseq_LRDD.R6 (reverse primer)
  • Alternative donor

Frame 0

AGGIPGAERPLSSHPLRAACSAASPGAAWTGLP
ACWDMSGLQGPSVGDGCNGGGARAGGSCCRRRCF
RGFGRRVQGAAFPGRQPAELGPVPRGLPAAAAP
VCPAASAAAAGGILASEHSRGPSAAGGHPGPAA
SEPVLPPLPGPQRRATPGHTGCLSPGCPDQPARW
SEWPGPSGPPGPELQQPGDTAGLCPADARSGCA
LAVSQLPL*AA*GSGGPPRPHLPHSDTQPPADA
APSTGGPIHPAAPRSLSESAGHATS*DWRPGQPP
GAQPGLQPAAEPPSLSGGTSVLAAPCPAQQPPG
LCAS*LGPPSTPHPARPEGQPAPGPAP*AARRP
LCAPAGEPPG*GLARRPEFTSGSPHSRNAQTVPD
LRFGQLSCDPSRLLSDPGLWRPPAVPSGSHRHP
HHHPLSAAAAGARPRPPGSS*RPAQPCAGAAAP
WGGLPAGCGAVAALHPTAGPALP*SGGQDPE*QQ
LG*PGDLPGGRGTPAALGSLPGAPLLLVPCGFP
PCVQCLPGATGGDTAVLLGSSWGQSHLPPWGH*
GASSSLHAGGAHGWPRAAGPPGRTRGCSEPPAVP
VTERSPQLPPTGHRAAASALWHHRPQSGPLPPA
PVVLGPSCSHLG*HHSSGGPGAHPPVRTLPGHT
LLLVLALVHHQELCGRPGSEGLGAAAAAPCEPHR
SAAAPGP*AGPAAVPAPKQGGRHPSAAAGAVPG
PRAL*HGGDVRGRRVLCGLRARHRRGC*PP*LC
GGQNLLCLLLAPEECEGGIRDHHSGPGGSGCAGP
GVLLPWRGACAGARGG*GCPAEEGRRRPVDGHS
AHQAAETSRVRGATAGGWPLLGTLESGRCRDRL
SDAEQPAECGWASGSGLASRGPAPGGVLPGGAAH
PARVPG*SG*ADPSHALLLG*APGWAARGCGAP
GAGPGAE*PAGRG*RGARSLGARPPQVPGQHPT
HGLGPQGPRSAWLLGSTAPRACPGLGPTDF*AGP
DIPQWMGRAPTFKSLQCVGTGPCEQQNCTVSFT
S

Frame 1

PAGSQAPSAR*AATLCAPPAAQLPRALPGQACL
RAGTCLASKDRRWAMAATVEGPELEAAAAAGDA
SEDSDAGSRALPFLGGNRLSLDLYPGGCQQLLHL
CVQQPLQLLQVEFLRLSTHEDPQLLEATLAQLP
QSLSCLRSLVLKGGQRRDTLGACLRGALTNLPA
GLSGLAHLAHLDLSFNSLETLPACVLQMRGLGAL
LLSHNCLSELPEALGALPALTFLTVTHNRLQTL
PPALGALSTLQRLDLSQNLLDTLPPEIGGLGSL
LELNLASNRLQSLPASLAGLRSLRLLVLHSNLLA
SVPADLARLPLLTRLDLRDNQLRDLPPELLDAP
FVRLQGNPLGEASPDAPSSPVAALIPEMPRLFL
TSDLDSFPVTPQGCSVTLACGVRLQFPAGATATP
ITIRYRLLLPEPGLVPLGPHDALLSHVLELQPH
GVAFQQDVGLWLLFTPPQARRCREVVVRTRNDN
SWGDLETYLEEEAPQRLWAHCQVPHFSWFLVVSR
PVSNACLVPPEGTLLCSSGHPGVKVIFPPGATE
EPRRVSMQVVRMAGRELQALLGEPEAAVSPLLC
LSQSGPPSFLQPVTVQLPLPSGITGLSLDRSRLH
LLYWAPPAATWDDITAQVVLELTHLYARFQVTH
FSWYWLWYTTKNCVGGLARKAWERLRLHRVNLI
ALQRRRDPEQVLLQCLPRNKVDATLRRLLERYRG
PEPSDTVEMFEGEEFFAAFERGIDVDADRPDCV
EGRICFVFYSHLKNVKEVYVTTTLDREAQAVRG
Q
VSFYRGAVPVRVPEEAEAARQRKGADALWMATL
PIKLPRLRGSEGPRRGAGLSLAPLNLGDAETGF
LTQSNLLSVAGRLGLDWPAVALHLGVSYREVQR
IRHEFRDDLDEQIRHMLFSWAERQAGQPGAVGLL
VQALEQSDRQDVAEEVRAVLELGRRKYQDSIRR
MGLAPKDPALPGSSAPQPPEPAQA*APQTFRLA
QIFPSGWAEPPPSSLSSVWGRVPVSNKTALFLSP

Frame 2

RRDPRRRAPAEQPPFARRLQRSFPGRCLDRPAC
VLGHVWPPRTVGGRWLQRWRGQSWRQLLPQEML
QRIRTQGPGRCLSWAATG*AWTCTPGAASSCCT
CVSSSLCSCCRWNSCV*ALTRTLSCWRPPWPSCL
RACPASAPWSSKEGNAGTHWVPVSGVP*PTCPL
V*VAWPIWPTWT*ASTAWRHCRPVSCRCEVWVR
SCCLTTASLSCLRLWGPSPPSPSSQ*HTTACRRC
PQHWGPYPPCSASISLRICWTRYLLRLEAWAAS
WSSTWPPTGCRASQPLWRDFGPCGSLSCTATSW
PLCQLTWPAFHSSPGST*GTTSSGTCPLSC*TPP
LCACRGTPWVRPRQTPRVHQWQPSFQKCPDCS*
PQIWTAFL*PLKAAQ*PWPVASACSSQREPPPP
PSPSAIGCCCRSQASSPWVLMTPCSAMCWSCSPM
GWPSSRMWGCGCSSPHRRPGAAVKWWSGPGMTT
AGVTWRPTWRKRHPSGSGLTARCPTSPGSLWFP
ALCPMPAWCHRRGHCCAPRVILGSKSSSPLGPLR
SLVESPCRWCAWLAESCRPSWENQRLQ*APCCA
CHRAVPPASSNRSPCSCLCPLASQASVWTAPAC
TCCTGPLLQPPGMTSQLRWSWSSPTCTHASRSHT
SPGTGSGTPPRTVWEAWLGRPGSGCGCTV*TSS
LCSGAGTLSRSCCSACPETRWTPPFGGCWSGTG
APSPLTRWRCSRAKSSLRPSSAASTWMLTALTVW
RAESALSSTRT*RM*RRYT*PPLWTGRLRLCGA
R
CPSTVARCLCGCPRRLRLPGRGRAQTPCGWPL
CPSSCRDFEGPRGHGGGLASPWHP*IWEMPRPAF
*RRATC*VWLGVWVWTGQPWPCTWGCPTGRCSA
SGTSSGMIWMSRSVTCSSPGLSARLGSQGLWGS
WCRPWSRVTGRTWLKRCAQSWSSAAASTRTASDA
WAWPPRTPLCLAPRLHSPQSLPRPRPHRLLGWP
RYSPVDGQSPHLQVSPVCGDGSL*ATKLHCFFH
L

Sequence NM_018494

GCCGGCGGGATCCCAGGCGCCGAGCGCCCGCTGAGCAGCCACCCTTTGCGCGCCGCCTGCAGCGCAGCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTG
CGTGCTGGGACATGTCTGGCCTCCAAGGACCGTCGGTGGGCGATGGCTGCAACGGTGGAGGGGCCAGAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTT
CAGAGGATTCGGACGCAGGGTCCAGGGCGCTGCCTTTCCTGGGCGGCAACCGGCTGAGCTTGGACCTGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCT
GTGTGTCCAGCAGCCTCTGCAGCTGCTGCAGGTGGAATTCTTGCGTCTGAGCACTCACGAGGACCCTCAGCTGCTGGAGGCCACCCTGGCCCAGCTGCCT
CAGAGCCTGTCCTGCCTCCGCTCCCTGGTCCTCAAAGGGTCGATCTGGGACCTCGGACCCTGGCTCTGAGGGCCACATCCGCCTCCCCCCTTCCCAGGAG
GGCAACGCCGGGACACACTGGGTGCCTGTCTCCGGGGTGCCCTGACCAACCTGCCCGCTGGTCTGAGTGGCCTGGCCCATCTGGCCCACCTGGACCTGAG
CTTCAACAGCCTGGAGACACTGCCGGCCTGTGTCCTGCAGATGCGAGGTCTGGGTGCGCTCTTGCTGTCTCACAACTGCCTCTCTGAGCTGCCTGAGGCT
CTGGGGGCCCTCCCCGCCCTCACCTTCCTCACAGTGACACACAACCGCCTGCAGACGCTGCCCCCAGCACTGGGGGCCCTATCCACCCTGCAGCGCCTCG
ATCTCTCTCAGAATCTGCTGGACACGCTACCTCCTGAGATTGGAGGCCTGGGCAGCCTCCTGGAGCTCAACCTGGCCTCCAACCGGCTGCAGAGCCTCCC
AGCCTCTCTGGCGGGACTTCGGTCCTTGCGGCTCCTTGTCCTGCACAGCAACCTCCTGGCCTCTGTGCCAGCTGACTTGGCCCGCCTTCCACTCCTCACC
CGGCTCGACCTGAGGGACAACCAGCTCCGGGACCTGCCCCCTGAGCTGCTAGACGCCCCCTTTGTGCGCCTGCAGGGGAACCCCCTGGGTGAGGCCTCGC
CAGACGCCCCGAGTTCACCAGTGGCAGCCCTCATTCCAGAAATGCCCAGACTGTTCCTGACCTCAGATTTGGACAGCTTTCCTGTGACCCCTCAAGGCTG
CTCAGTGACCCTGGCCTGTGGCGTCCGCCTGCAGTTCCCAGCGGGAGCCACCGCCACCCCCATCACCATCCGCTATCGGCTGCTGCTGCCGGAGCCAGGC
CTCGTCCCCCTGGGTCCTCATGACGCCCTGCTCAGCCATGTGCTGGAGCTGCAGCCCCATGGGGTGGCCTTCCAGCAGGATGTGGGGCTGTGGCTGCTCT
TCACCCCACCGCAGGCCCGGCGCTGCCGTGAAGTGGTGGTCAGGACCCGGAATGACAACAGCTGGGGTGACCTGGAGACCTACCTGGAGGAAGAGGCACC
CCAGCGGCTCTGGGCTCACTGCCAGGTGCCCCACTTCTCCTGGTTCCTTGTGGTTTCCCGCCCTGTGTCCAATGCCTGCCTGGTGCCACCGGAGGGGACA
CTGCTGTGCTCCTCGGGTCATCCTGGGGTCAAAGTCATCTTCCCCCCTGGGGCCACTGAGGAGCCTCGTCGAGTCTCCATGCAGGTGGTGCGCATGGCTG
GCCGAGAGCTGCAGGCCCTCCTGGGAGAACCAGAGGCTGCAGTGAGCCCCCTGCTGTGCCTGTCACAGAGCGGTCCCCCCAGCTTCCTCCAACCGGTCAC
CGTGCAGCTGCCTCTGCCCTCTGGCATCACAGGCCTCAGTCTGGACCGCTCCCGCCTGCACCTGTTGTACTGGGCCCCTCCTGCAGCCACCTGGGATGAC
ATCACAGCTCAGGTGGTCCTGGAGCTCACCCACCTGTACTGGCTCTGGTACACCACCAAGAACTGTGTGGGAGGCCTGGCTCGGAAGGCCTGGGAGCGGC
TGCGGCTGCACCGTGTGAACCTCATCGCTCTGCAGCGGCGCCGGGACCCTGAGCAGGTCCTGCTGCAGTGCCTGCCCCGAAACAAGGTGGACGCCACCCT
TCGGCGGCTGCTGGAGCGGTACCGGGGCCCCGAGCCCTCTGACACGGTGGAGATGTTCGAGGGCGAAGAGTTCTTTGCGGCCTTCGAGCGCGGCATCGAC
GTGGATGCTGACCGCCCTGACTGTGTGGAGGGCAGAATCTGCTTTGTCTTCTACTCGCACCTGAAGAATGTGAAGGAGGTATACGTGACCACCACTCTGG
ACCGGGAGGCTCAGGCTGTGCGGGGCCAG
GTGTCCTTCTACCGTGGCGCGGTGCCTGTGCGGGTGCCCGAGGAGGCTGAGGCTGCCCGGCAGAGGAAGGG
CGCAGACGCCCTGTGGATGGCCACTCTGCCCATCAAGCTGCCGAGACTTCGAGGGTCCGAGGGGCCACGGCGGGGGGCTGGCCTCTCCTTGGCACCCTTG
AATCTGGGAGATGCCGAGACCGGCTTTCTGACGCAGAGCAACCTGCTGAGTGTGGCTGGGCGTCTGGGTCTGGACTGGCCAGCCGTGGCCCTGCACCTGG
GGGTGTCCTACCGGGAGGTGCAGCGCATCCGGCACGAGTTCCGGGATGATCTGGATGAGCAGATCCGTCACATGCTCTTCTCCTGGGCTGAGCGCCAGGC
TGGGCAGCCAGGGGCTGTGGGGCTCCTGGTGCAGGCCCTGGAGCAGAGTGACCGGCAGGACGTGGCTGAAGAGGTGCGCGCAGTCTTGGAGCTCGGCCGC
CGCAAGTACCAGGACAGCATCCGACGCATGGGCTTGGCCCCCAAGGACCCCGCTCTGCCTGGCTCCTCGGCTCCACAGCCCCCAGAGCCTGCCCAGGCCT
AGGCCCCACAGACTTTTAGGCTGGCCCAGATATTCCCCAGTGGATGGGCAGAGCCCCCACCTTCAAGTCTCTCCAGTGTGTGGGGACGGGTCCCTGTGAG
CAACAAAACTGCACTGTTTCTTTCACCTCA
  • refseq_LRDD.F6 (forward primer)
  • refseq_LRDD.R6 (reverse primer)
  • Alternative donor

Frame 0

AGGIPGAERPLSSHPLRAACSAASPGAAWTGLP
ACWDMSGLQGPSVGDGCNGGGARAGGSCCRRRCF
RGFGRRVQGAAFPGRQPAELGPVPRGLPAAAAP
VCPAASAAAAGGILASEHSRGPSAAGGHPGPAA
SEPVLPPLPGPQRVDLGPRTLALRATSASPLPRR
ATPGHTGCLSPGCPDQPARWSEWPGPSGPPGPE
LQQPGDTAGLCPADARSGCALAVSQLPL*AA*G
SGGPPRPHLPHSDTQPPADAAPSTGGPIHPAAPR
SLSESAGHATS*DWRPGQPPGAQPGLQPAAEPP
SLSGGTSVLAAPCPAQQPPGLCAS*LGPPSTPH
PARPEGQPAPGPAP*AARRPLCAPAGEPPG*GLA
RRPEFTSGSPHSRNAQTVPDLRFGQLSCDPSRL
LSDPGLWRPPAVPSGSHRHPHHHPLSAAAAGAR
PRPPGSS*RPAQPCAGAAAPWGGLPAGCGAVAAL
HPTAGPALP*SGGQDPE*QQLG*PGDLPGGRGT
PAALGSLPGAPLLLVPCGFPPCVQCLPGATGGD
TAVLLGSSWGQSHLPPWGH*GASSSLHAGGAHGW
PRAAGPPGRTRGCSEPPAVPVTERSPQLPPTGH
RAAASALWHHRPQSGPLPPAPVVLGPSCSHLG*
HHSSGGPGAHPPVLALVHHQELCGRPGSEGLGAA
AAAPCEPHRSAAAPGP*AGPAAVPAPKQGGRHP
SAAAGAVPGPRAL*HGGDVRGRRVLCGLRARHR
RGC*PP*LCGGQNLLCLLLAPEECEGGIRDHHSG
PGGSGCAGP
GVLLPWRGACAGARGG*GCPAEEG
RRRPVDGHSAHQAAETSRVRGATAGGWPLLGTL
ESGRCRDRLSDAEQPAECGWASGSGLASRGPAPG
GVLPGGAAHPARVPG*SG*ADPSHALLLG*APG
WAARGCGAPGAGPGAE*PAGRG*RGARSLGARP
PQVPGQHPTHGLGPQGPRSAWLLGSTAPRACPGL
GPTDF*AGPDIPQWMGRAPTFKSLQCVGTGPCE
QQNCTVSFTS

Frame 1

PAGSQAPSAR*AATLCAPPAAQLPRALPGQACL
RAGTCLASKDRRWAMAATVEGPELEAAAAAGDA
SEDSDAGSRALPFLGGNRLSLDLYPGGCQQLLHL
CVQQPLQLLQVEFLRLSTHEDPQLLEATLAQLP
QSLSCLRSLVLKGSIWDLGPWL*GPHPPPPFPG
GQRRDTLGACLRGALTNLPAGLSGLAHLAHLDLS
FNSLETLPACVLQMRGLGALLLSHNCLSELPEA
LGALPALTFLTVTHNRLQTLPPALGALSTLQRL
DLSQNLLDTLPPEIGGLGSLLELNLASNRLQSLP
ASLAGLRSLRLLVLHSNLLASVPADLARLPLLT
RLDLRDNQLRDLPPELLDAPFVRLQGNPLGEAS
PDAPSSPVAALIPEMPRLFLTSDLDSFPVTPQGC
SVTLACGVRLQFPAGATATPITIRYRLLLPEPG
LVPLGPHDALLSHVLELQPHGVAFQQDVGLWLL
FTPPQARRCREVVVRTRNDNSWGDLETYLEEEAP
QRLWAHCQVPHFSWFLVVSRPVSNACLVPPEGT
LLCSSGHPGVKVIFPPGATEEPRRVSMQVVRMA
GRELQALLGEPEAAVSPLLCLSQSGPPSFLQPVT
VQLPLPSGITGLSLDRSRLHLLYWAPPAATWDD
ITAQVVLELTHLYWLWYTTKNCVGGLARKAWER
LRLHRVNLIALQRRRDPEQVLLQCLPRNKVDATL
RRLLERYRGPEPSDTVEMFEGEEFFAAFERGID
VDADRPDCVEGRICFVFYSHLKNVKEVYVTTTL
DREAQAVRGQ
VSFYRGAVPVRVPEEAEAARQRKG
ADALWMATLPIKLPRLRGSEGPRRGAGLSLAPL
NLGDAETGFLTQSNLLSVAGRLGLDWPAVALHL
GVSYREVQRIRHEFRDDLDEQIRHMLFSWAERQA
GQPGAVGLLVQALEQSDRQDVAEEVRAVLELGR
RKYQDSIRRMGLAPKDPALPGSSAPQPPEPAQA
*APQTFRLAQIFPSGWAEPPPSSLSSVWGRVPVS
NKTALFLSP

Frame 2

RRDPRRRAPAEQPPFARRLQRSFPGRCLDRPAC
VLGHVWPPRTVGGRWLQRWRGQSWRQLLPQEML
QRIRTQGPGRCLSWAATG*AWTCTPGAASSCCT
CVSSSLCSCCRWNSCV*ALTRTLSCWRPPWPSCL
RACPASAPWSSKGRSGTSDPGSEGHIRLPPSQE
GNAGTHWVPVSGVP*PTCPLV*VAWPIWPTWT*
ASTAWRHCRPVSCRCEVWVRSCCLTTASLSCLRL
WGPSPPSPSSQ*HTTACRRCPQHWGPYPPCSAS
ISLRICWTRYLLRLEAWAASWSSTWPPTGCRAS
QPLWRDFGPCGSLSCTATSWPLCQLTWPAFHSSP
GST*GTTSSGTCPLSC*TPPLCACRGTPWVRPR
QTPRVHQWQPSFQKCPDCS*PQIWTAFL*PLKA
AQ*PWPVASACSSQREPPPPPSPSAIGCCCRSQA
SSPWVLMTPCSAMCWSCSPMGWPSSRMWGCGCS
SPHRRPGAAVKWWSGPGMTTAGVTWRPTWRKRH
PSGSGLTARCPTSPGSLWFPALCPMPAWCHRRGH
CCAPRVILGSKSSSPLGPLRSLVESPCRWCAWL
AESCRPSWENQRLQ*APCCACHRAVPPASSNRS
PCSCLCPLASQASVWTAPACTCCTGPLLQPPGMT
SQLRWSWSSPTCTGSGTPPRTVWEAWLGRPGSG
CGCTV*TSSLCSGAGTLSRSCCSACPETRWTPP
FGGCWSGTGAPSPLTRWRCSRAKSSLRPSSAAST
WMLTALTVWRAESALSSTRT*RM*RRYT*PPLW
TGRLRLCGAR
CPSTVARCLCGCPRRLRLPGRGR
AQTPCGWPLCPSSCRDFEGPRGHGGGLASPWHP*
IWEMPRPAF*RRATC*VWLGVWVWTGQPWPCTW
GCPTGRCSASGTSSGMIWMSRSVTCSSPGLSAR
LGSQGLWGSWCRPWSRVTGRTWLKRCAQSWSSAA
ASTRTASDAWAWPPRTPLCLAPRLHSPQSLPRP
RPHRLLGWPRYSPVDGQSPHLQVSPVCGDGSL*
ATKLHCFFHL

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