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Sequences

Sequence NM_207297

GTTGGTACTAAGAAGTGCCTTTCCTGACGTCTCTGCTGCTTGGAACCGCTTCTAGAGCAGTCTCTGCTTTTGCCTTGCTTGCTGCCAGCTAGACTGTGAC GACAGCACATCCACCCTCCACCTCTAGCCCAGACACCCCCATTTCTACTTATAATCAAGAGAAAAGCTCTAAGTATCTGGCATTGCCCTAGGCTGCTTTA GTGTTAAAAGAAAAGTTTGCTGAAAAAGTAAGATATCTTCTGCCAGGAAATCAAGGAGGAAAAAAAAAATCATTTTCTCGATTTTGCTCTAAACTGCTGC ATCTGTCTATGCCAAACTAATCAATACCGATTGCACCACCAAACTCCATTGCAAATTCAGCTGTGAGGAGATTCCCTTTCAGACAACTTTGCTGAAAGCA GCTTGGAAATTCGGTGTCGAAGGGTCTGCCACGTTTTCATGCTTGCATTTTGGGCTCCAAATTGGCACTGGGAAGGGGTTACTGAGAGCACAAGGCTGAT ACCAGGCCCTACTTTTAAACGTTCATCTACTTACAATCCTAGTATTTCTCTAAAAACCAAAACCTCTTTGAATTAACAGTTTCATGCTGTGAATTTCTAG TGGGAGATCTTTTCCTTGATATTGACGACACAATTTTCCATGTACTTTTAAAGCAGGGAGTGGGGAAAAGTATTTTGAGGGGACATTTTCATCATCAGTT CAGCTTTTTTTTTTTGGTTGTTGCTCTTTTTTGGGGGGGTTGGGTTTGTTGGTTTCACTGAAACATTTAACTACCTGTAAAATCTAAACATGGCTGTTAG TGTCACACCAATTCGGGACACAAAATGGCTAACACTGGAAGTATGTAGAGAGTTCCAGAGGGGGACTTGCTCACGGCCAGACACGGAATGTAAATTTGCA CATCCTTCGAAAAGCTGCCAAGTTGAAAATGGACGAGTAATCGCCTGCTTTGATTCATTGAAAGGCCGTTGCTCCAGGGAGAACTGCAAATATCTTCATC CACCCCCACATTTAAAAACGCAGTTGGAGATAAATGGACGCAATAACTTGATTCAGCAGAAGAACATGGCCATGTTGGCCCAGCAAATGCAACTAGCCAA TGCCATGATGCCTGGTGCCCCATTACAACCCGTGCCAATGTTTTCAGTTGCACCAAGCTTAGCCACCAATGCATCAGCAGCCGCCTTTAATCCCTATCTG GGACCTGTTTCTCCAAGCCTGGTCCCGGCAGAGATCTTGCCGACTGCACCAATGTTGGTTACAGGGAATCCGGGTGTCCCTGTACCTGCAGCTGCTGCAG CTGCTGCACAGAAATTAATGCGAACAGACAGACTTGAGGTATGTCGAGAGTACCAACGTGGCAATTGCAACCGAGGAGAAAATGATTGTCGGTTTGCTCA TCCTGCTGACAGCACAATGATTGACACCAATGACAACACAGTCACTGTGTGTATGGATTACATCAAAGGGAGATGCTCTCGGGAAAAGTGCAAATACTTT CATCCCCCTGCACATTTGCAAGCCAAGATCAAGGCTGCCCAATACCAGGTCAACCAGGCTGCAGCTGCACAGGCTGCAGCCACCGCAGCTGCCATGGGAA TTCCTCAAGCTGTACTTCCCCCATTACCAAAGAGGCCTGCTCTTGAAAAAACCAACGGTGCCACCGCAGTCTTTAACACTGGTATTTTCCAATACCAACA GGCTCTAGCCAACATGCAGTTACAACAGCATACAGCATTTCTCCCACCAGGCTCAATATTGTGCATGACACCCGCTACAAGTGTTGATACCCATAATATC TGCCGAACATCTGACTAGCCACAAGTATGTTACCCAGATGTAGAATTTTCATCACTAAACAATCATGCTAAAGAGGAAAGGACAGTGTGCTTGGTTAGAG TAAAGGACGAGGTCATTAGCCATATTGTATATATCGTCAAGCAACACACACAAAAGTTCCTCAGCCACAAGACATCCACATATTGCATGTTAACCAGAAG AAAAGACAACATTTTCCGGAAATCCACTGCACACTGTTGCCTATACACTTTGTACATTTAATTGATATTTGTGCTGAGGTGATATTCCTGTCTAAAAGAA CAACATTGTCTTTCTTTTCTAGCACAGAGTTATGCATTCAAAGATGCATACCTAGTTAGTTTCCTATATATTCATGCCATCTTGAAAAGACAGACTATGG TGTAACCATGATTCTATTATGTATTGGTACGTCTGTAGACCAAGATATAATTTTTTAAAAATAAGTTTATTTCTTTCAAGGTTTACAAATAACAAAGGTG CACCTTGTATTTAAAATTGCCATTATAGATGAGAGCGTGCATGCACAGTCATTTTTGTTTAAGAGTAATATTTTTAATGTAATAGATTGTAAGACGTGGT GAGGGAGGGATCTGACAGAGATGAATGTGCCAAGCAAAACCACAACTGTGTATATTTTAAAGCACATCATGGCTTTAAGTACCATGTTGTTAAGGATTCT CATGAAGTGCCATAGACTGTACATCAAATTAGAGTATTATTTCTTCAGTGTTATTGTTTTCAGAGCCACATTTTGTTGCATATTTGCTAGTACTAATCAG TCAAAGGGCACCATTCTTTTTTTTTTTTTTTGAAACCAAAGCTGTCTCAGAAATGGCCAATTTAACTTTACAGTAACAATAGACAGCACAACACAAACTC TCTCAATACAGATAAACTCACACATACTGGAGATATATATATAATAGATATATATAAAATTATTTTAATGCATTGTAGTGTAATATTTATGCATACTATA CTGTATAACATGTTATTCAAAAGGGATTGCCATTTCTGAGACACAGTAACAAAAAAATGAGGAAATTATTTTGCTTCTATTTATAGCCTCTGTCAAAAGT CAAAAGACTATAAATGCTTTGCAAAAATGGTTTCACGTTTGCTTAAATGCTTCATCACAGTCACATTCAAAATAGTGACTCTAAACAAAGAAGAAAGCAG CACTGTCATCAGATGCATGATAAACCAAAATATGAAAATGGGAAATGTTTAATTAACCTAGTAATTGGGTGGGTTAAGTACATGGGTGAATTTTATATGT GATTTTTGTTTTGTTTTGTTTTGTTCAGATTAACTGCTTATAGCCTTAGAAAGCCTTTTACAAAATTAAAAAAAAAATAGATGTGCATTCAGTTTTTAAG AATGGAATCATCCAAAGGAATTCCTTTTTTTGAGGTTTGGATGTTGCAGCTAGTAAAGGATATTTTTGCTCTGTTCAGCAGTTCTAAAAATTGCTGAAGT AGGGGCCAGGTCACTGGTAGTTATAGTATGGAATGGGAGAAGTGAAAGTTCAGTTATAGAACTTTCCATACTTCCAAGTTTACTGCAAGTTTTTATGCTT GAGAGAGATGCTTTCTAATATAAGACTGATGTGTTGATTTTACTGATTGTACTGTACATCTATTAAAGCCTTAGATTATTACATTACGGGTTGGAACCCA TACCAATGTAATTTCAATCGTGTTAAGAAAGTAATGGTGACTTCACATGTTATTGTAGTTAGTTACATTATAGAATATTACTTATTTTTCTTGTTAAAAT GTAGTTTTTCATTTCCTACATTTATTAGATTTTCATTTTCTATTAACAATTGAATACCATTTCAGTTTATAGACTTGTTTTATTAGATTTTACCAATGAA TTTTTCAAAATACAAAAAAAAGTAGTTTTTCCTTCATAACATACTCAGTTTTGAATTACATGTAGTGTCACATGAATATTCGTATTGTTAACTAAATGAT TTATATTTTACTGATTTAATATTACAGTGTAAGAATGTCAGTCATTGTTAGTTCTTGTCTAGTTTTCATTAAAAGAACAAAGATCTTTTATATGGATATC TTATAAATATATAATCATTGCTAAGTAAGAAGTTAAGTTGTTGCTATCGCAACAATCCTGGCAGACAATTGAGTAATATTTTGATGATTTATTTTGTTTG TAATTAGTTATTATAAGAAGATCTAGATCCTAGATATTAGAATAAAATTTATTTTCTACTGTATCCATTTCAAATGTTAAAATATTGTTTAATATTTTTG AAATCCCTGAGTATCAGGCCTTGTTATAAATAAGCTGCATAATCAATAAATAGAACAAGGGACTTTTTGTTGATAATCCAAATACTCAAAGTTTACGTAA TGAAAATTATAGCGTGTGTGCAAACTCTTGAGGGTTGATTATGCTGCAATTTAGCATGTTGGAACGTCTAGGGAGAAGGTTGACTTTTTGCACTTCTGTA TATAGTCAAAAGAGAGAAACCTGTATAATAGTAAGATCTTATTTTGAATAAAAACGTCTATAATTACAAGGAGTTTTGTTAAGGCTAATACAATGACAGA CTGAGCAAAATTGCTTGCAAAAGTGGCACAGAGTTAGCACTCCATACCCCTTCAAACATGTTGCTTTGCTTTCTTGTGGACAGCTTGTAGTTTGCCAGGA TTTTTTCAGCTGGAAAGATACGCCATCCTTTCAAACCCTCATGACTGACAAAAACTCCATGGGGCCAAATCTGCCTGAAGATCATTACCAAAAATAGCAG GTACTTCTACCATTAAGGTGAAATCATGGATCAGATATTCCTTACATTTTTCAAAACTACTGCATGTTTAAAACTTCAACAAAAAAAGAGAGAAAGAACT ATACTAAGAACATATATTATTCAGATCAGTTTCTGCCAATTTCAGTGGTTTATTGTTCACAAAAAAATCTTCAAAACAAGTATTGACTTTCACAAAATTT AAATCATAAACAGGCAAACCAAACAGCACACTGTAGCTATAGTTGTTATGTGATTGTTTTTTAATTGCTGTAGGATCCTGTTCTTTCAGCAGGTGAAAAA TAAAACGCAGTTCAAATTTCATGGTTTTAATTTTCAACTCAGAAGCACTCAAAAATGCAAAATGTGATAATGGGCACTTGTTTAAAAGAATTAGTGTATC CAGCCTTCACTCCAGCTGGTTAAAAATGTTGCACTTATCAGCAACCCTACCACTTTCATCTGCTGAAAGGACAAATGTGCTTGGTTTTACTATTATGTAA TCACAACTTACTTTCTGCTTGTAGTTGCTTAAAATTATGTATTTTGTCTTGGGCTGCAATTTGTTTTATGCTTATTTTATTATTACTGCAGTAGTTGACT TTGCTGTATGGAAAAATAAAGTGAAATTGCCCTAATAAAACTTCTCTTTCTTAAGTA

refseq_MBNL1.F3 (forward primer)
refseq_MBNL1.R3 (reverse primer)
Single exon skipping

Frame 0

VGTKKCLS*RLCCLEPLLEQSLLLPCLLPARL* RQHIHPPPLAQTPPFLLIIKRKALSIWHCPRLL* C*KKSLLKK*DIFCQEIKEEKKNHFLDFALNCC ICLCQTNQYRLHHQTPLQIQL*GDSLSDNFAES SLEIRCRRVCHVFMLAFWAPNWHWEGVTESTRLI PGPTFKRSSTYNPSISLKTKTSLN*QFHAVNF* WEIFSLILTTQFSMYF*SREWGKVF*GDIFIIS SAFFFWLLLFFGGVGFVGFTETFNYL*NLNMAVS VTPIRDTKWLTLEVCREFQRGTCSRPDTECKFA HPSKSCQVENGRVIACFDSLKGRCSRENCKYLH PPPHLKTQLEINGRNNLIQQKNMAMLAQQMQLAN AMMPGAPLQPVPMFSVAPSLATNASAAAFNPYL GPVSPSLVPAEILPTAPMLVTGNPGVPVPAAAA AAAQKLMRTDRLEVCREYQRGNCNRGENDCRFAH PADSTMIDTNDNTVTVCMDYIKGRCSREKCKYF HPPAHLQAKIKAAQYQVNQAAAAQAAATAAAMG IPQAVLPPLPKRPALEKTNGATAVFNTGIFQYQQ ALANMQLQQHTAFLPPGSILCMTPATSVDTHNI CRTSD*PQVCYPDVEFSSLNNHAKEERTVCLVR VKDEVISHIVYIVKQHTQKFLSHKTSTYCMLTRR KDNIFRKSTAHCCLYTLYI*LIFVLR*YSCLKE QHCLSFLAQSYAFKDAYLVSFLYIHAILKRQTM V*P*FYYVLVRL*TKI*FFKNKFISFKVYK*QRC TLYLKLPL*MRACMHSHFCLRVIFLM**IVRRG EGGI*QR*MCQAKPQLCIF*STSWL*VPCC*GF S*SAIDCTSN*SIISSVLLFSEPHFVAYLLVLIS QRAPFFFFFFETKAVSEMANLTLQ*Q*TAQHKL SQYR*THTYWRYIYNRYI*NYFNAL*CNIYAYY TV*HVIQKGLPFLRHSNKKMRKLFCFYL*PLSKV KRL*MLCKNGFTFA*MLHHSHIQNSDSKQRRKQ HCHQMHDKPKYENGKCLINLVIGWVKYMGEFYM *FLFCFVLFRLTAYSLRKPFTKLKKK*MCIQFLR MESSKGIPFFEVWMLQLVKDIFALFSSSKNC*S RGQVTGSYSMEWEK*KFSYRTFHTSKFTASFYA *ERCFLI*D*CVDFTDCTVHLLKP*IITLRVGTH TNVISIVLRK*W*LHMLL*LVTL*NITYFSC*N VVFHFLHLLDFHFLLTIEYHFSL*TCFIRFYQ* IFQNTKKSSFSFITYSVLNYM*CHMNIRIVN*MI YILLI*YYSVRMSVIVSSCLVFIKRTKIFYMDI L*IYNHC*VRS*VVAIATILADN*VIF**FILF VISYYKKI*ILDIRIKFIFYCIHFKC*NIV*YF* NP*VSGLVINKLHNQ*IEQGTFC**SKYSKFT* *KL*RVCKLLRVDYAAI*HVGTSREKVDFLHFC I*SKERNLYNSKILF*IKTSIITRSFVKANTMTD *AKLLAKVAQS*HSIPLQTCCFAFLWTACSLPG FFQLERYAILSNPHD*QKLHGAKSA*RSLPKIA GTSTIKVKSWIRYSLHFSKLLHV*NFNKKREKEL Y*EHILFRSVSANFSGLLFTKKSSKQVLTFTKF KS*TGKPNSTL*L*LLCDCFLIAVGSCSFSR*K IKRSSNFMVLIFNSEALKNAKCDNGHLFKRISVS SLHSSWLKMLHLSATLPLSSAERTNVLGFTIM* SQLTFCL*LLKIMYFVLGCNLFYAYFIITAVVD FAVWKNKVKLP**NFSFLS

Frame 1

LVLRSAFPDVSAAWNRF*SSLCFCLACCQLDCD DSTSTLHL*PRHPHFYL*SREKL*VSGIALGCF SVKRKVC*KSKISSARKSRRKKKIIFSILL*TAA SVYAKLINTDCTTKLHCKFSCEEIPFQTTLLKA AWKFGVEGSATFSCLHFGLQIGTGKGLLRAQG* YQALLLNVHLLTILVFL*KPKPL*INSFML*ISS GRSFP*Y*RHNFPCTFKAGSGEKYFEGTFSSSV QLFFFGCCSFLGGLGLLVSLKHLTTCKI*TWLL VSHQFGTQNG*HWKYVESSRGGLAHGQTRNVNLH ILRKAAKLKMDE*SPALIH*KAVAPGRTANIFI HPHI*KRSWR*MDAIT*FSRRTWPCWPSKCN*P MP*CLVPHYNPCQCFQLHQA*PPMHQQPPLIPIW DLFLQAWSRQRSCRLHQCWLQGIRVSLYLQLLQ LLHRN*CEQTDLRYVESTNVAIATEEKMIVGLL ILLTAQ*LTPMTTQSLCVWITSKGDALGKSANTF IPLHICKPRSRLPNTRSTRLQLHRLQPPQLPWE FLKLYFPHYQRGLLLKKPTVPPQSLTLVFSNTN RL*PTCSYNSIQHFSHQAQYCA*HPLQVLIPIIS AEHLTSHKYVTQM*NFHH*TIMLKRKGQCAWLE *RTRSLAILYISSSNTHKSSSATRHPHIAC*PE EKTTFSGNPLHTVAYTLCTFN*YLC*GDIPV*KN NIVFLF*HRVMHSKMHT*LVSYIFMPS*KDRLW CNHDSIMYWYVCRPRYNFLKISLFLSRFTNNKG APCI*NCHYR*ERACTVIFV*E*YF*CNRL*DVV REGSDRDECAKQNHNCVYFKAHHGFKYHVVKDS HEVP*TVHQIRVLFLQCYCFQSHILLHIC*Y*S VKGHHSFFFFLKPKLSQKWPI*LYSNNRQHNTNS LNTDKLTHTGDIYIIDIYKIILMHCSVIFMHTI LYNMLFKRDCHF*DTVTKK*GNYFASIYSLCQK SKDYKCFAKMVSRLLKCFITVTFKIVTLNKEESS TVIRCMINQNMKMGNV*LT**LGGLSTWVNFIC DFCFVLFCSD*LLIALESLLQN*KKNRCAFSF* EWNHPKEFLFLRFGCCS**RIFLLCSAVLKIAEV GARSLVVIVWNGRSESSVIELSILPSLLQVFML ERDAF*YKTDVLILLIVLYIY*SLRLLHYGLEP IPM*FQSC*ESNGDFTCYCS*LHYRILLIFLVKM *FFISYIY*IFIFY*QLNTISVYRLVLLDFTNE FFKIQKKVVFPS*HTQF*ITCSVT*IFVLLTK* FIFY*FNITV*ECQSLLVLV*FSLKEQRSFIWIS YKYIIIAK*EVKLLLSQQSWQTIE*YFDDLFCL *LVIIRRSRS*ILE*NLFSTVSISNVKILFNIF EIPEYQALL*ISCIINK*NKGLFVDNPNTQSLRN ENYSVCANS*GLIMLQFSMLERLGRRLTFCTSV YSQKRETCIIVRSYFE*KRL*LQGVLLRLIQ*Q TEQNCLQKWHRVSTPYPFKHVALLSCGQLVVCQD FFSWKDTPSFQTLMTDKNSMGPNLPEDHYQK*Q VLLPLR*NHGSDIPYIFQNYCMFKTSTKKERKN YTKNIYYSDQFLPISVVYCSQKNLQNKY*LSQNL NHKQANQTAHCSYSCYVIVF*LL*DPVLSAGEK *NAVQISWF*FSTQKHSKMQNVIMGTCLKELVY PAFTPAG*KCCTYQQPYHFHLLKGQMCLVLLLCN HNLLSACSCLKLCILSWAAICFMLILLLLQ*LT LLYGKIK*NCPNKTSLS*V

Frame 2

WY*EVPFLTSLLLGTASRAVSAFALLAAS*TVT TAHPPSTSSPDTPISTYNQEKSSKYLALP*AAL VLKEKFAEKVRYLLPGNQGGKKKSFSRFCSKLL HLSMPN*SIPIAPPNSIANSAVRRFPFRQLC*KQ LGNSVSKGLPRFHACILGSKLALGRGY*EHKAD TRPYF*TFIYLQS*YFSKNQNLFELTVSCCEFL VGDLFLDIDDTIFHVLLKQGVGKSILRGHFHHQF SFFFLVVALFWGGWVCWFH*NI*LPVKSKHGC* CHTNSGHKMANTGSM*RVPEGDLLTARHGM*IC TSFEKLPS*KWTSNRLL*FIERPLLQGELQISSS TPTFKNAVGDKWTQ*LDSAEEHGHVGPANATSQ CHDAWCPITTRANVFSCTKLSHQCISSRL*SLS GTCFSKPGPGRDLADCTNVGYRESGCPCTCSCCS CCTEINANRQT*GMSRVPTWQLQPRRK*LSVCS SC*QHND*HQ*QHSHCVYGLHQREMLSGKVQIL SSPCTFASQDQGCPIPGQPGCSCTGCSHRSCHGN SSSCTSPITKEACS*KNQRCHRSL*HWYFPIPT GSSQHAVTTAYSISPTRLNIVHDTRYKC*YP*Y LPNI*LATSMLPRCRIFITKQSC*RGKDSVLG*S KGRGH*PYCIYRQATHTKVPQPQDIHILHVNQK KRQHFPEIHCTLLPIHFVHLIDICAEVIFLSKR TTLSFFSSTELCIQRCIPS*FPIYSCHLEKTDYG VTMILLCIGTSVDQDIIF*K*VYFFQGLQITKV HLVFKIAIIDESVHAQSFLFKSNIFNVIDCKTW *GRDLTEMNVPSKTTTVYILKHIMALSTMLLRIL MKCHRLYIKLEYYFFSVIVFRATFCCIFASTNQ SKGTILFFFF*NQSCLRNGQFNFTVTIDSTTQT LSIQINSHILEIYI**IYIKLF*CIVV*YLCILY CITCYSKGIAISETQ*QKNEEIILLLFIASVKS QKTINALQKWFHVCLNASSQSHSK**L*TKKKA ALSSDA**TKI*KWEMFN*PSNWVG*VHG*ILYV IFVLFCFVQINCL*P*KAFYKIKKKIDVHSVFK NGIIQRNSFF*GLDVAASKGYFCSVQQF*KLLK *GPGHW*L*YGMGEVKVQL*NFPYFQVYCKFLCL REMLSNIRLMC*FY*LYCTSIKALDYYITGWNP YQCNFNRVKKVMVTSHVIVVSYIIEYYLFFLLK CSFSFPTFIRFSFSINN*IPFQFIDLFY*ILPMN FSKYKKK*FFLHNILSFELHVVSHEYSYC*LND LYFTDLILQCKNVSHC*FLSSFH*KNKDLLYGY LINI*SLLSKKLSCCYRNNPGRQLSNILMIYFVC N*LL*EDLDPRY*NKIYFLLYPFQMLKYCLIFL KSLSIRPCYK*AA*SINRTRDFLLIIQILKVYV MKIIACVQTLEG*LCCNLACWNV*GEG*LFALLY IVKREKPV***DLILNKNVYNYKEFC*G*YNDR LSKIACKSGTELALHTPSNMLLCFLVDSL*FAR IFSAGKIRHPFKPS*LTKTPWGQICLKIITKNSR YFYH*GEIMDQIFLTFFKTTACLKLQQKKRERT ILRTYIIQISFCQFQWFIVHKKIFKTSIDFHKI *IINRQTKQHTVAIVVM*LFFNCCRILFFQQVKN KTQFKFHGFNFQLRSTQKCKM**WALV*KN*CI QPSLQLVKNVALISNPTTFIC*KDKCAWFYYYV ITTYFLLVVA*NYVFCLGLQFVLCLFYYYCSS*L CCMEK*SEIALIKLLFLK

Sequence NM_207295

GTTGGTACTAAGAAGTGCCTTTCCTGACGTCTCTGCTGCTTGGAACCGCTTCTAGAGCAGTCTCTGCTTTTGCCTTGCTTGCTGCCAGCTAGACTGTGAC GACAGCACATCCACCCTCCACCTCTAGCCCAGACACCCCCATTTCTACTTATAATCAAGAGAAAAGCTCTAAGTATCTGGCATTGCCCTAGGCTGCTTTA GTGTTAAAAGAAAAGTTTGCTGAAAAAGTAAGATATCTTCTGCCAGGAAATCAAGGAGGAAAAAAAAAATCATTTTCTCGATTTTGCTCTAAACTGCTGC ATCTGTCTATGCCAAACTAATCAATACCGATTGCACCACCAAACTCCATTGCAAATTCAGCTGTGAGGAGATTCCCTTTCAGACAACTTTGCTGAAAGCA GCTTGGAAATTCGGTGTCGAAGGGTCTGCCACGTTTTCATGCTTGCATTTTGGGCTCCAAATTGGCACTGGGAAGGGGTTACTGAGAGCACAAGGCTGAT ACCAGGCCCTACTTTTAAACGTTCATCTACTTACAATCCTAGTATTTCTCTAAAAACCAAAACCTCTTTGAATTAACAGTTTCATGCTGTGAATTTCTAG TGGGAGATCTTTTCCTTGATATTGACGACACAATTTTCCATGTACTTTTAAAGCAGGGAGTGGGGAAAAGTATTTTGAGGGGACATTTTCATCATCAGTT CAGCTTTTTTTTTTTGGTTGTTGCTCTTTTTTGGGGGGGTTGGGTTTGTTGGTTTCACTGAAACATTTAACTACCTGTAAAATCTAAACATGGCTGTTAG TGTCACACCAATTCGGGACACAAAATGGCTAACACTGGAAGTATGTAGAGAGTTCCAGAGGGGGACTTGCTCACGGCCAGACACGGAATGTAAATTTGCA CATCCTTCGAAAAGCTGCCAAGTTGAAAATGGACGAGTAATCGCCTGCTTTGATTCATTGAAAGGCCGTTGCTCCAGGGAGAACTGCAAATATCTTCATC CACCCCCACATTTAAAAACGCAGTTGGAGATAAATGGACGCAATAACTTGATTCAGCAGAAGAACATGGCCATGTTGGCCCAGCAAATGCAACTAGCCAA TGCCATGATGCCTGGTGCCCCATTACAACCCGTGGTATGTCGAGAGTACCAACGTGGCAATTGCAACCGAGGAGAAAATGATTGTCGGTTTGCTCATCCT GCTGACAGCACAATGATTGACACCAATGACAACACAGTCACTGTGTGTATGGATTACATCAAAGGGAGATGCTCTCGGGAAAAGTGCAAATACTTTCATC CCCCTGCACATTTGCAAGCCAAGATCAAGGCTGCCCAATACCAGGTCAACCAGGCTGCAGCTGCACAGGCTGCAGCCACCGCAGCTGCCATGGGAATTCC TCAAGCTGTACTTCCCCCATTACCAAAGAGGCCTGCTCTTGAAAAAACCAACGGTGCCACCGCAGTCTTTAACACTGGTATTTTCCAATACCAACAGGCT CTAGCCAACATGCAGTTACAACAGCATACAGCATTTCTCCCACCAGGCTCAATATTGTGCATGACACCCGCTACAAGTGTTGTTCCCATGGTGCACGGTG CTACGCCAGCCACTGTGTCCGCAGCAACAACATCTGCCACAAGTGTTCCCTTCGCTGCAACAGCCACAGCCAACCAGATACCCATAATATCTGCCGAACA TCTGACTAGCCACAAGTATGTTACCCAGATGTAGAATTTTCATCACTAAACAATCATGCTAAAGAGGAAAGGACAGTGTGCTTGGTTAGAGTAAAGGACG AGGTCATTAGCCATATTGTATATATCGTCAAGCAACACACACAAAAGTTCCTCAGCCACAAGACATCCACATATTGCATGTTAACCAGAAGAAAAGACAA CATTTTCCGGAAATCCACTGCACACTGTTGCCTATACACTTTGTACATTTAATTGATATTTGTGCTGAGGTGATATTCCTGTCTAAAAGAACAACATTGT CTTTCTTTTCTAGCACAGAGTTATGCATTCAAAGATGCATACCTAGTTAGTTTCCTATATATTCATGCCATCTTGAAAAGACAGACTATGGTGTAACCAT GATTCTATTATGTATTGGTACGTCTGTAGACCAAGATATAATTTTTTAAAAATAAGTTTATTTCTTTCAAGGTTTACAAATAACAAAGGTGCACCTTGTA TTTAAAATTGCCATTATAGATGAGAGCGTGCATGCACAGTCATTTTTGTTTAAGAGTAATATTTTTAATGTAATAGATTGTAAGACGTGGTGAGGGAGGG ATCTGACAGAGATGAATGTGCCAAGCAAAACCACAACTGTGTATATTTTAAAGCACATCATGGCTTTAAGTACCATGTTGTTAAGGATTCTCATGAAGTG CCATAGACTGTACATCAAATTAGAGTATTATTTCTTCAGTGTTATTGTTTTCAGAGCCACATTTTGTTGCATATTTGCTAGTACTAATCAGTCAAAGGGC ACCATTCTTTTTTTTTTTTTTTGAAACCAAAGCTGTCTCAGAAATGGCCAATTTAACTTTACAGTAACAATAGACAGCACAACACAAACTCTCTCAATAC AGATAAACTCACACATACTGGAGATATATATATAATAGATATATATAAAATTATTTTAATGCATTGTAGTGTAATATTTATGCATACTATACTGTATAAC ATGTTATTCAAAAGGGATTGCCATTTCTGAGACACAGTAACAAAAAAATGAGGAAATTATTTTGCTTCTATTTATAGCCTCTGTCAAAAGTCAAAAGACT ATAAATGCTTTGCAAAAATGGTTTCACGTTTGCTTAAATGCTTCATCACAGTCACATTCAAAATAGTGACTCTAAACAAAGAAGAAAGCAGCACTGTCAT CAGATGCATGATAAACCAAAATATGAAAATGGGAAATGTTTAATTAACCTAGTAATTGGGTGGGTTAAGTACATGGGTGAATTTTATATGTGATTTTTGT TTTGTTTTGTTTTGTTCAGATTAACTGCTTATAGCCTTAGAAAGCCTTTTACAAAATTAAAAAAAAAATAGATGTGCATTCAGTTTTTAAGAATGGAATC ATCCAAAGGAATTCCTTTTTTTGAGGTTTGGATGTTGCAGCTAGTAAAGGATATTTTTGCTCTGTTCAGCAGTTCTAAAAATTGCTGAAGTAGGGGCCAG GTCACTGGTAGTTATAGTATGGAATGGGAGAAGTGAAAGTTCAGTTATAGAACTTTCCATACTTCCAAGTTTACTGCAAGTTTTTATGCTTGAGAGAGAT GCTTTCTAATATAAGACTGATGTGTTGATTTTACTGATTGTACTGTACATCTATTAAAGCCTTAGATTATTACATTACGGGTTGGAACCCATACCAATGT AATTTCAATCGTGTTAAGAAAGTAATGGTGACTTCACATGTTATTGTAGTTAGTTACATTATAGAATATTACTTATTTTTCTTGTTAAAATGTAGTTTTT CATTTCCTACATTTATTAGATTTTCATTTTCTATTAACAATTGAATACCATTTCAGTTTATAGACTTGTTTTATTAGATTTTACCAATGAATTTTTCAAA ATACAAAAAAAAGTAGTTTTTCCTTCATAACATACTCAGTTTTGAATTACATGTAGTGTCACATGAATATTCGTATTGTTAACTAAATGATTTATATTTT ACTGATTTAATATTACAGTGTAAGAATGTCAGTCATTGTTAGTTCTTGTCTAGTTTTCATTAAAAGAACAAAGATCTTTTATATGGATATCTTATAAATA TATAATCATTGCTAAGTAAGAAGTTAAGTTGTTGCTATCGCAACAATCCTGGCAGACAATTGAGTAATATTTTGATGATTTATTTTGTTTGTAATTAGTT ATTATAAGAAGATCTAGATCCTAGATATTAGAATAAAATTTATTTTCTACTGTATCCATTTCAAATGTTAAAATATTGTTTAATATTTTTGAAATCCCTG AGTATCAGGCCTTGTTATAAATAAGCTGCATAATCAATAAATAGAACAAGGGACTTTTTGTTGATAATCCAAATACTCAAAGTTTACGTAATGAAAATTA TAGCGTGTGTGCAAACTCTTGAGGGTTGATTATGCTGCAATTTAGCATGTTGGAACGTCTAGGGAGAAGGTTGACTTTTTGCACTTCTGTATATAGTCAA AAGAGAGAAACCTGTATAATAGTAAGATCTTATTTTGAATAAAAACGTCTATAATTACAAGGAGTTTTGTTAAGGCTAATACAATGACAGACTGAGCAAA ATTGCTTGCAAAAGTGGCACAGAGTTAGCACTCCATACCCCTTCAAACATGTTGCTTTGCTTTCTTGTGGACAGCTTGTAGTTTGCCAGGATTTTTTCAG CTGGAAAGATACGCCATCCTTTCAAACCCTCATGACTGACAAAAACTCCATGGGGCCAAATCTGCCTGAAGATCATTACCAAAAATAGCAGGTACTTCTA CCATTAAGGTGAAATCATGGATCAGATATTCCTTACATTTTTCAAAACTACTGCATGTTTAAAACTTCAACAAAAAAAGAGAGAAAGAACTATACTAAGA ACATATATTATTCAGATCAGTTTCTGCCAATTTCAGTGGTTTATTGTTCACAAAAAAATCTTCAAAACAAGTATTGACTTTCACAAAATTTAAATCATAA ACAGGCAAACCAAACAGCACACTGTAGCTATAGTTGTTATGTGATTGTTTTTTAATTGCTGTAGGATCCTGTTCTTTCAGCAGGTGAAAAATAAAACGCA GTTCAAATTTCATGGTTTTAATTTTCAACTCAGAAGCACTCAAAAATGCAAAATGTGATAATGGGCACTTGTTTAAAAGAATTAGTGTATCCAGCCTTCA CTCCAGCTGGTTAAAAATGTTGCACTTATCAGCAACCCTACCACTTTCATCTGCTGAAAGGACAAATGTGCTTGGTTTTACTATTATGTAATCACAACTT ACTTTCTGCTTGTAGTTGCTTAAAATTATGTATTTTGTCTTGGGCTGCAATTTGTTTTATGCTTATTTTATTATTACTGCAGTAGTTGACTTTGCTGTAT GGAAAAATAAAGTGAAATTGCCCTAATAAAACTTCTCTTTCTTAAGTA

refseq_MBNL1.F3 (forward primer)
refseq_MBNL1.R3 (reverse primer)
Single exon skipping

Frame 0

VGTKKCLS*RLCCLEPLLEQSLLLPCLLPARL* RQHIHPPPLAQTPPFLLIIKRKALSIWHCPRLL* C*KKSLLKK*DIFCQEIKEEKKNHFLDFALNCC ICLCQTNQYRLHHQTPLQIQL*GDSLSDNFAES SLEIRCRRVCHVFMLAFWAPNWHWEGVTESTRLI PGPTFKRSSTYNPSISLKTKTSLN*QFHAVNF* WEIFSLILTTQFSMYF*SREWGKVF*GDIFIIS SAFFFWLLLFFGGVGFVGFTETFNYL*NLNMAVS VTPIRDTKWLTLEVCREFQRGTCSRPDTECKFA HPSKSCQVENGRVIACFDSLKGRCSRENCKYLH PPPHLKTQLEINGRNNLIQQKNMAMLAQQMQLAN AMMPGAPLQPVVCREYQRGNCNRGENDCRFAHP ADSTMIDTNDNTVTVCMDYIKGRCSREKCKYFH PPAHLQAKIKAAQYQVNQAAAAQAAATAAAMGIP QAVLPPLPKRPALEKTNGATAVFNTGIFQYQQA LANMQLQQHTAFLPPGSILCMTPATSVVPMVHG ATPATVSAATTSATSVPFAATATANQIPIISAEH LTSHKYVTQM*NFHH*TIMLKRKGQCAWLE*RT RSLAILYISSSNTHKSSSATRHPHIAC*PEEKT TFSGNPLHTVAYTLCTFN*YLC*GDIPV*KNNIV FLF*HRVMHSKMHT*LVSYIFMPS*KDRLWCNH DSIMYWYVCRPRYNFLKISLFLSRFTNNKGAPC I*NCHYR*ERACTVIFV*E*YF*CNRL*DVVREG SDRDECAKQNHNCVYFKAHHGFKYHVVKDSHEV P*TVHQIRVLFLQCYCFQSHILLHIC*Y*SVKG HHSFFFFLKPKLSQKWPI*LYSNNRQHNTNSLNT DKLTHTGDIYIIDIYKIILMHCSVIFMHTILYN MLFKRDCHF*DTVTKK*GNYFASIYSLCQKSKD YKCFAKMVSRLLKCFITVTFKIVTLNKEESSTVI RCMINQNMKMGNV*LT**LGGLSTWVNFICDFC FVLFCSD*LLIALESLLQN*KKNRCAFSF*EWN HPKEFLFLRFGCCS**RIFLLCSAVLKIAEVGAR SLVVIVWNGRSESSVIELSILPSLLQVFMLERD AF*YKTDVLILLIVLYIY*SLRLLHYGLEPIPM *FQSC*ESNGDFTCYCS*LHYRILLIFLVKM*FF ISYIY*IFIFY*QLNTISVYRLVLLDFTNEFFK IQKKVVFPS*HTQF*ITCSVT*IFVLLTK*FIF Y*FNITV*ECQSLLVLV*FSLKEQRSFIWISYKY IIIAK*EVKLLLSQQSWQTIE*YFDDLFCL*LV IIRRSRS*ILE*NLFSTVSISNVKILFNIFEIP EYQALL*ISCIINK*NKGLFVDNPNTQSLRNENY SVCANS*GLIMLQFSMLERLGRRLTFCTSVYSQ KRETCIIVRSYFE*KRL*LQGVLLRLIQ*QTEQ NCLQKWHRVSTPYPFKHVALLSCGQLVVCQDFFS WKDTPSFQTLMTDKNSMGPNLPEDHYQK*QVLL PLR*NHGSDIPYIFQNYCMFKTSTKKERKNYTK NIYYSDQFLPISVVYCSQKNLQNKY*LSQNLNHK QANQTAHCSYSCYVIVF*LL*DPVLSAGEK*NA VQISWF*FSTQKHSKMQNVIMGTCLKELVYPAF TPAG*KCCTYQQPYHFHLLKGQMCLVLLLCNHNL LSACSCLKLCILSWAAICFMLILLLLQ*LTLLY GKIK*NCPNKTSLS*V

Frame 1

LVLRSAFPDVSAAWNRF*SSLCFCLACCQLDCD DSTSTLHL*PRHPHFYL*SREKL*VSGIALGCF SVKRKVC*KSKISSARKSRRKKKIIFSILL*TAA SVYAKLINTDCTTKLHCKFSCEEIPFQTTLLKA AWKFGVEGSATFSCLHFGLQIGTGKGLLRAQG* YQALLLNVHLLTILVFL*KPKPL*INSFML*ISS GRSFP*Y*RHNFPCTFKAGSGEKYFEGTFSSSV QLFFFGCCSFLGGLGLLVSLKHLTTCKI*TWLL VSHQFGTQNG*HWKYVESSRGGLAHGQTRNVNLH ILRKAAKLKMDE*SPALIH*KAVAPGRTANIFI HPHI*KRSWR*MDAIT*FSRRTWPCWPSKCN*P MP*CLVPHYNPWYVESTNVAIATEEKMIVGLLIL LTAQ*LTPMTTQSLCVWITSKGDALGKSANTFI PLHICKPRSRLPNTRSTRLQLHRLQPPQLPWEF LKLYFPHYQRGLLLKKPTVPPQSLTLVFSNTNRL *PTCSYNSIQHFSHQAQYCA*HPLQVLFPWCTV LRQPLCPQQQHLPQVFPSLQQPQPTRYP*YLPN I*LATSMLPRCRIFITKQSC*RGKDSVLG*SKGR GH*PYCIYRQATHTKVPQPQDIHILHVNQKKRQ HFPEIHCTLLPIHFVHLIDICAEVIFLSKRTTL SFFSSTELCIQRCIPS*FPIYSCHLEKTDYGVTM ILLCIGTSVDQDIIF*K*VYFFQGLQITKVHLV FKIAIIDESVHAQSFLFKSNIFNVIDCKTW*GR DLTEMNVPSKTTTVYILKHIMALSTMLLRILMKC HRLYIKLEYYFFSVIVFRATFCCIFASTNQSKG TILFFFF*NQSCLRNGQFNFTVTIDSTTQTLSI QINSHILEIYI**IYIKLF*CIVV*YLCILYCIT CYSKGIAISETQ*QKNEEIILLLFIASVKSQKT INALQKWFHVCLNASSQSHSK**L*TKKKAALS SDA**TKI*KWEMFN*PSNWVG*VHG*ILYVIFV LFCFVQINCL*P*KAFYKIKKKIDVHSVFKNGI IQRNSFF*GLDVAASKGYFCSVQQF*KLLK*GP GHW*L*YGMGEVKVQL*NFPYFQVYCKFLCLREM LSNIRLMC*FY*LYCTSIKALDYYITGWNPYQC NFNRVKKVMVTSHVIVVSYIIEYYLFFLLKCSF SFPTFIRFSFSINN*IPFQFIDLFY*ILPMNFSK YKKK*FFLHNILSFELHVVSHEYSYC*LNDLYF TDLILQCKNVSHC*FLSSFH*KNKDLLYGYLIN I*SLLSKKLSCCYRNNPGRQLSNILMIYFVCN*L L*EDLDPRY*NKIYFLLYPFQMLKYCLIFLKSL SIRPCYK*AA*SINRTRDFLLIIQILKVYVMKI IACVQTLEG*LCCNLACWNV*GEG*LFALLYIVK REKPV***DLILNKNVYNYKEFC*G*YNDRLSK IACKSGTELALHTPSNMLLCFLVDSL*FARIFS AGKIRHPFKPS*LTKTPWGQICLKIITKNSRYFY H*GEIMDQIFLTFFKTTACLKLQQKKRERTILR TYIIQISFCQFQWFIVHKKIFKTSIDFHKI*II NRQTKQHTVAIVVM*LFFNCCRILFFQQVKNKTQ FKFHGFNFQLRSTQKCKM**WALV*KN*CIQPS LQLVKNVALISNPTTFIC*KDKCAWFYYYVITT YFLLVVA*NYVFCLGLQFVLCLFYYYCSS*LCCM EK*SEIALIKLLFLK

Frame 2

WY*EVPFLTSLLLGTASRAVSAFALLAAS*TVT TAHPPSTSSPDTPISTYNQEKSSKYLALP*AAL VLKEKFAEKVRYLLPGNQGGKKKSFSRFCSKLL HLSMPN*SIPIAPPNSIANSAVRRFPFRQLC*KQ LGNSVSKGLPRFHACILGSKLALGRGY*EHKAD TRPYF*TFIYLQS*YFSKNQNLFELTVSCCEFL VGDLFLDIDDTIFHVLLKQGVGKSILRGHFHHQF SFFFLVVALFWGGWVCWFH*NI*LPVKSKHGC* CHTNSGHKMANTGSM*RVPEGDLLTARHGM*IC TSFEKLPS*KWTSNRLL*FIERPLLQGELQISSS TPTFKNAVGDKWTQ*LDSAEEHGHVGPANATSQ CHDAWCPITTRGMSRVPTWQLQPRRK*LSVCSS C*QHND*HQ*QHSHCVYGLHQREMLSGKVQILSS PCTFASQDQGCPIPGQPGCSCTGCSHRSCHGNS SSCTSPITKEACS*KNQRCHRSL*HWYFPIPTG SSQHAVTTAYSISPTRLNIVHDTRYKCCSHGARC YASHCVRSNNICHKCSLRCNSHSQPDTHNICRT SD*PQVCYPDVEFSSLNNHAKEERTVCLVRVKD EVISHIVYIVKQHTQKFLSHKTSTYCMLTRRKDN IFRKSTAHCCLYTLYI*LIFVLR*YSCLKEQHC LSFLAQSYAFKDAYLVSFLYIHAILKRQTMV*P *FYYVLVRL*TKI*FFKNKFISFKVYK*QRCTLY LKLPL*MRACMHSHFCLRVIFLM**IVRRGEGG I*QR*MCQAKPQLCIF*STSWL*VPCC*GFS*S AIDCTSN*SIISSVLLFSEPHFVAYLLVLISQRA PFFFFFFETKAVSEMANLTLQ*Q*TAQHKLSQY R*THTYWRYIYNRYI*NYFNAL*CNIYAYYTV* HVIQKGLPFLRHSNKKMRKLFCFYL*PLSKVKRL *MLCKNGFTFA*MLHHSHIQNSDSKQRRKQHCH QMHDKPKYENGKCLINLVIGWVKYMGEFYM*FL FCFVLFRLTAYSLRKPFTKLKKK*MCIQFLRMES SKGIPFFEVWMLQLVKDIFALFSSSKNC*SRGQ VTGSYSMEWEK*KFSYRTFHTSKFTASFYA*ER CFLI*D*CVDFTDCTVHLLKP*IITLRVGTHTNV ISIVLRK*W*LHMLL*LVTL*NITYFSC*NVVF HFLHLLDFHFLLTIEYHFSL*TCFIRFYQ*IFQ NTKKSSFSFITYSVLNYM*CHMNIRIVN*MIYIL LI*YYSVRMSVIVSSCLVFIKRTKIFYMDIL*I YNHC*VRS*VVAIATILADN*VIF**FILFVIS YYKKI*ILDIRIKFIFYCIHFKC*NIV*YF*NP* VSGLVINKLHNQ*IEQGTFC**SKYSKFT**KL *RVCKLLRVDYAAI*HVGTSREKVDFLHFCI*S KERNLYNSKILF*IKTSIITRSFVKANTMTD*AK LLAKVAQS*HSIPLQTCCFAFLWTACSLPGFFQ LERYAILSNPHD*QKLHGAKSA*RSLPKIAGTS TIKVKSWIRYSLHFSKLLHV*NFNKKREKELY*E HILFRSVSANFSGLLFTKKSSKQVLTFTKFKS* TGKPNSTL*L*LLCDCFLIAVGSCSFSR*KIKR SSNFMVLIFNSEALKNAKCDNGHLFKRISVSSLH SSWLKMLHLSATLPLSSAERTNVLGFTIM*SQL TFCL*LLKIMYFVLGCNLFYAYFIITAVVDFAV WKNKVKLP**NFSFLS

Sequence NM_207292

TTACATCTTATAGCACCCAGTGAATTATGATATGGGGCTGCGGTTTATTTGCTCTGGCGACAATATGGCTTACTTGCCGTGGAATATCCCGGTATTTCAG AAGTAACATATTATTTTTAAAGAGGGAGCTTTGCTTTCTCTAAATCACATGGTGCAAAGTTGGGAATACAGTGGCCTGTAAATTACAAATATTATTTACA AACTAGAAGGAAAGCTGACATGTGTTTGTGCCTGTGTGACAGTTTCACTAAGGAATTCCAAAACTGTGGATAGCCTGTTACTTGAGAAAAATACACGGTG GCAGTCTTGCAGATGTTTTTCCCGCCTGTTAGCCCTCCAGGTTCAAAAACTGTTTGGTGCATTTTAAGGCGGCAGTCTCACCAGATCGCAGGATTCTAGT CTAATTGGCTTCTAACCTTCAATCTAACCTTGCCGGCTTCCTTGCAAAGCCCGGTGCAAGGGCCTCTTTCAAAATGAACCCACTGGTGTGCCTAGCAGTC GGTAGAAGAAGCGGGAGGGCGTCCGGTCTGCACGCCCGCCGCGAGGTTACAATGCTGAACGCATGAGATGGAAGATACCAACGGGAGGCCGAGGGGATCC ACGGCGCCCGCGCGGGCTCCGGCTTCCTCCTGCTCTCGGCGCCGCTGGGCGACCGCCCATGACCCGCTCTTGCGGGCTCTGTCCGGTTGACAGGCGACCC TGTGGCCCGGGGAAGCGCGGGAGGGCGCCGGCGGAAAGTTGAAGAGCGTTTTTCTCGCCGCCGCGTGCATTAGGAGCTCGACGAGTCCGCCCTGGGCTTC CTGGTGGGGCTGGGCGGGCGGGGGAGGGGCCGCGCAGCAGCAGCGGAAGCCAGACCTCGGCGATAAGAGGCTGCACAGCGACATGCAACAGTCTTTTCAC TGCAGCTGAATGAGTTGTGGCGCCCACAATGCTCCCATGACAAGGAGCTGACAAGTTCCATTTTCCGTCGCGGGCATCTTGGAATCATGACTCCCACAAT GCCTTGGGCACTTGGTCGACAGTGGGGCCGCCTCTGAAAAAAAAATGTGAGAGGTTGGTACTAAGAAGTGCCTTTCCTGACGTCTCTGCTGCTTGGAACC GCTTCTAGAGCAGTCTCTGCTTTTGCCTTGCTTGCTGCCAGCTAGACTGTGACGACAGCACATCCACCCTCCACCTCTAGCCCAGACACCCCCATTTCTA CTTATAATCAAGAGAAAAGCTCTAAGTATCTGGCATTGCCCTAGGCTGCTTTAGTGTTAAAAGAAAAGTTTGCTGAAAAAGTAAGATATCTTCTGCCAGG AAATCAAGGAGGAAAAAAAAAATCATTTTCTCGATTTTGCTCTAAACTGCTGCATCTGTCTATGCCAAACTAATCAATACCGATTGCACCACCAAACTCC ATTGCAAATTCAGCTGTGAGGAGATTCCCTTTCAGACAACTTTGCTGAAAGCAGCTTGGAAATTCGGTGTCGAAGGGTCTGCCACGTTTTCATGCTTGCA TTTTGGGCTCCAAATTGGCACTGGGAAGGGGTTACTGAGAGCACAAGGCTGATACCAGGCCCTACTTTTAAACGTTCATCTACTTACAATCCTAGTATTT CTCTAAAAACCAAAACCTCTTTGAATTAACAGTTTCATGCTGTGAATTTCTAGTGGGAGATCTTTTCCTTGATATTGACGACACAATTTTCCATGTACTT TTAAAGCAGGGAGTGGGGAAAAGTATTTTGAGGGGACATTTTCATCATCAGTTCAGCTTTTTTTTTTTGGTTGTTGCTCTTTTTTGGGGGGGTTGGGTTT GTTGGTTTCACTGAAACATTTAACTACCTGTAAAATCTAAACATGGCTGTTAGTGTCACACCAATTCGGGACACAAAATGGCTAACACTGGAAGTATGTA GAGAGTTCCAGAGGGGGACTTGCTCACGGCCAGACACGGAATGTAAATTTGCACATCCTTCGAAAAGCTGCCAAGTTGAAAATGGACGAGTAATCGCCTG CTTTGATTCATTGAAAGGCCGTTGCTCCAGGGAGAACTGCAAATATCTTCATCCACCCCCACATTTAAAAACGCAGTTGGAGATAAATGGACGCAATAAC TTGATTCAGCAGAAGAACATGGCCATGTTGGCCCAGCAAATGCAACTAGCCAATGCCATGATGCCTGGTGCCCCATTACAACCCGTGCCAATGTTTTCAG TTGCACCAAGCTTAGCCACCAATGCATCAGCAGCCGCCTTTAATCCCTATCTGGGACCTGTTTCTCCAAGCCTGGTCCCGGCAGAGATCTTGCCGACTGC ACCAATGTTGGTTACAGGGAATCCGGGTGTCCCTGTACCTGCAGCTGCTGCAGCTGCTGCACAGAAATTAATGCGAACAGACAGACTTGAGGTATGTCGA GAGTACCAACGTGGCAATTGCAACCGAGGAGAAAATGATTGTCGGTTTGCTCATCCTGCTGACAGCACAATGATTGACACCAATGACAACACAGTCACTG TGTGTATGGATTACATCAAAGGGAGATGCTCTCGGGAAAAGTGCAAATACTTTCATCCCCCTGCACATTTGCAAGCCAAGATCAAGGCTGCCCAATACCA GGTCAACCAGGCTGCAGCTGCACAGGCTGCAGCCACCGCAGCTGCCATGGGAATTCCTCAAGCTGTACTTCCCCCATTACCAAAGAGGCCTGCTCTTGAA AAAACCAACGGTGCCACCGCAGTCTTTAACACTGGTATTTTCCAATACCAACAGGCTCTAGCCAACATGCAGTTACAACAGCATACAGCATTTCTCCCAC CAGTTCCCATGGTGCACGGTGCTACGCCAGCCACTGTGTCCGCAGCAACAACATCTGCCACAAGTGTTCCCTTCGCTGCAACAGCCACAGCCAACCAGAT ACCCATAATATCTGCCGAACATCTGACTAGCCACAAGTATGTTACCCAGATGTAGAATTTTCATCACTAAACAATCATGCTAAAGAGGAAAGGACAGTGT GCTTGGTTAGAGTAAAGGACGAGGTCATTAGCCATATTGTATATATCGTCAAGCAACACACACAAAAGTTCCTCAGCCACAAGACATCCACATATTGCAT GTTAACCAGAAGAAAAGACAACATTTTCCGGAAATCCACTGCACACTGTTGCCTATACACTTTGTACATTTAATTGATATTTGTGCTGAGGTGATATTCC TGTCTAAAAGAACAACATTGTCTTTCTTTTCTAGCACAGAGTTATGCATTCAAAGATGCATACCTAGTTAGTTTCCTATATATTCATGCCATCTTGAAAA GACAGACTATGGTGTAACCATGATTCTATTATGTATTGGTACGTCTGTAGACCAAGATATAATTTTTTAAAAATAAGTTTATTTCTTTCAAGGTTTACAA ATAACAAAGGTGCACCTTGTATTTAAAATTGCCATTATAGATGAGAGCGTGCATGCACAGTCATTTTTGTTTAAGAGTAATATTTTTAATGTAATAGATT GTAAGACGTGGTGAGGGAGGGATCTGACAGAGATGAATGTGCCAAGCAAAACCACAACTGTGTATATTTTAAAGCACATCATGGCTTTAAGTACCATGTT GTTAAGGATTCTCATGAAGTGCCATAGACTGTACATCAAATTAGAGTATTATTTCTTCAGTGTTATTGTTTTCAGAGCCACATTTTGTTGCATATTTGCT AGTACTAATCAGTCAAAGGGCACCATTCTTTTTTTTTTTTTTTGAAACCAAAGCTGTCTCAGAAATGGCCAATTTAACTTTACAGTAACAATAGACAGCA CAACACAAACTCTCTCAATACAGATAAACTCACACATACTGGAGATATATATATAATAGATATATATAAAATTATTTTAATGCATTGTAGTGTAATATTT ATGCATACTATACTGTATAACATGTTATTCAAAAGGGATTGCCATTTCTGAGACACAGTAACAAAAAAATGAGGAAATTATTTTGCTTCTATTTATAGCC TCTGTCAAAAGTCAAAAGACTATAAATGCTTTGCAAAAATGGTTTCACGTTTGCTTAAATGCTTCATCACAGTCACATTCAAAATAGTGACTCTAAACAA AGAAGAAAGCAGCACTGTCATCAGATGCATGATAAACCAAAATATGAAAATGGGAAATGTTTAATTAACCTAGTAATTGGGTGGGTTAAGTACATGGGTG AATTTTATATGTGATTTTTGTTTTGTTTTGTTTTGTTCAGATTAACTGCTTATAGCCTTAGAAAGCCTTTTACAAAATTAAAAAAAAAATAGATGTGCAT TCAGTTTTTAAGAATGGAATCATCCAAAGGAATTCCTTTTTTTGAGGTTTGGATGTTGCAGCTAGTAAAGGATATTTTTGCTCTGTTCAGCAGTTCTAAA AATTGCTGAAGTAGGGGCCAGGTCACTGGTAGTTATAGTATGGAATGGGAGAAGTGAAAGTTCAGTTATAGAACTTTCCATACTTCCAAGTTTACTGCAA GTTTTTATGCTTGAGAGAGATGCTTTCTAATATAAGACTGATGTGTTGATTTTACTGATTGTACTGTACATCTATTAAAGCCTTAGATTATTACATTACG GGTTGGAACCCATACCAATGTAATTTCAATCGTGTTAAGAAAGTAATGGTGACTTCACATGTTATTGTAGTTAGTTACATTATAGAATATTACTTATTTT TCTTGTTAAAATGTAGTTTTTCATTTCCTACATTTATTAGATTTTCATTTTCTATTAACAATTGAATACCATTTCAGTTTATAGACTTGTTTTATTAGAT TTTACCAATGAATTTTTCAAAATACAAAAAAAAGTAGTTTTTCCTTCATAACATACTCAGTTTTGAATTACATGTAGTGTCACATGAATATTCGTATTGT TAACTAAATGATTTATATTTTACTGATTTAATATTACAGTGTAAGAATGTCAGTCATTGTTAGTTCTTGTCTAGTTTTCATTAAAAGAACAAAGATCTTT TATATGGATATCTTATAAATATATAATCATTGCTAAGTAAGAAGTTAAGTTGTTGCTATCGCAACAATCCTGGCAGACAATTGAGTAATATTTTGATGAT TTATTTTGTTTGTAATTAGTTATTATAAGAAGATCTAGATCCTAGATATTAGAATAAAATTTATTTTCTACTGTATCCATTTCAAATGTTAAAATATTGT TTAATATTTTTGAAATCCCTGAGTATCAGGCCTTGTTATAAATAAGCTGCATAATCAATAAATAGAACAAGGGACTTTTTGTTGATAATCCAAATACTCA AAGTTTACGTAATGAAAATTATAGCGTGTGTGCAAACTCTTGAGGGTTGATTATGCTGCAATTTAGCATGTTGGAACGTCTAGGGAGAAGGTTGACTTTT TGCACTTCTGTATATAGTCAAAAGAGAGAAACCTGTATAATAGTAAGATCTTATTTTGAATAAAAACGTCTATAATTACAAGGAGTTTTGTTAAGGCTAA TACAATGACAGACTGAGCAAAATTGCTTGCAAAAGTGGCACAGAGTTAGCACTCCATACCCCTTCAAACATGTTGCTTTGCTTTCTTGTGGACAGCTTGT AGTTTGCCAGGATTTTTTCAGCTGGAAAGATACGCCATCCTTTCAAACCCTCATGACTGACAAAAACTCCATGGGGCCAAATCTGCCTGAAGATCATTAC CAAAAATAGCAGGTACTTCTACCATTAAGGTGAAATCATGGATCAGATATTCCTTACATTTTTCAAAACTACTGCATGTTTAAAACTTCAACAAAAAAAG AGAGAAAGAACTATACTAAGAACATATATTATTCAGATCAGTTTCTGCCAATTTCAGTGGTTTATTGTTCACAAAAAAATCTTCAAAACAAGTATTGACT TTCACAAAATTTAAATCATAAACAGGCAAACCAAACAGCACACTGTAGCTATAGTTGTTATGTGATTGTTTTTTAATTGCTGTAGGATCCTGTTCTTTCA GCAGGTGAAAAATAAAACGCAGTTCAAATTTCATGGTTTTAATTTTCAACTCAGAAGCACTCAAAAATGCAAAATGTGATAATGGGCACTTGTTTAAAAG AATTAGTGTATCCAGCCTTCACTCCAGCTGGTTAAAAATGTTGCACTTATCAGCAACCCTACCACTTTCATCTGCTGAAAGGACAAATGTGCTTGGTTTT ACTATTATGTAATCACAACTTACTTTCTGCTTGTAGTTGCTTAAAATTATGTATTTTGTCTTGGGCTGCAATTTGTTTTATGCTTATTTTATTATTACTG CAGTAGTTGACTTTGCTGTATGGAAAAATAAAGTGAAATTGCCCTAATAAAACTTCTCTTTCTTAAGTA

refseq_MBNL1.F3 (forward primer)
refseq_MBNL1.R3 (reverse primer)
Single exon skipping

Frame 0

LHLIAPSEL*YGAAVYLLWRQYGLLAVEYPGIS EVTYYF*RGSFAFSKSHGAKLGIQWPVNYKYYLQ TRRKADMCLCLCDSFTKEFQNCG*PVT*EKYTV AVLQMFFPPVSPPGSKTVWCILRRQSHQIAGF* SNWLLTFNLTLPASLQSPVQGPLSK*THWCA*QS VEEAGGRPVCTPAARLQC*THEMEDTNGRPRGS TAPARAPASSCSRRRWATAHDPLLRALSG*QAT LWPGEAREGAGGKLKSVFLAAACIRSSTSPPWAS WWGWAGGGGAAQQQRKPDLGDKRLHSDMQQSFH CS*MSCGAHNAPMTRS*QVPFSVAGILES*LPQ CLGHLVDSGAASEKKM*EVGTKKCLS*RLCCLEP LLEQSLLLPCLLPARL*RQHIHPPPLAQTPPFL LIIKRKALSIWHCPRLL*C*KKSLLKK*DIFCQ EIKEEKKNHFLDFALNCCICLCQTNQYRLHHQTP LQIQL*GDSLSDNFAESSLEIRCRRVCHVFMLA FWAPNWHWEGVTESTRLIPGPTFKRSSTYNPSI SLKTKTSLN*QFHAVNF*WEIFSLILTTQFSMYF *SREWGKVF*GDIFIISSAFFFWLLLFFGGVGF VGFTETFNYL*NLNMAVSVTPIRDTKWLTLEVC REFQRGTCSRPDTECKFAHPSKSCQVENGRVIAC FDSLKGRCSRENCKYLHPPPHLKTQLEINGRNN LIQQKNMAMLAQQMQLANAMMPGAPLQPVPMFS VAPSLATNASAAAFNPYLGPVSPSLVPAEILPTA PMLVTGNPGVPVPAAAAAAAQKLMRTDRLEVCR EYQRGNCNRGENDCRFAHPADSTMIDTNDNTVT VCMDYIKGRCSREKCKYFHPPAHLQAKIKAAQYQ VNQAAAAQAAATAAAMGIPQAVLPPLPKRPALE KTNGATAVFNTGIFQYQQALANMQLQQHTAFLP PVPMVHGATPATVSAATTSATSVPFAATATANQI PIISAEHLTSHKYVTQM*NFHH*TIMLKRKGQC AWLE*RTRSLAILYISSSNTHKSSSATRHPHIA C*PEEKTTFSGNPLHTVAYTLCTFN*YLC*GDIP V*KNNIVFLF*HRVMHSKMHT*LVSYIFMPS*K DRLWCNHDSIMYWYVCRPRYNFLKISLFLSRFT NNKGAPCI*NCHYR*ERACTVIFV*E*YF*CNRL *DVVREGSDRDECAKQNHNCVYFKAHHGFKYHV VKDSHEVP*TVHQIRVLFLQCYCFQSHILLHIC *Y*SVKGHHSFFFFLKPKLSQKWPI*LYSNNRQH NTNSLNTDKLTHTGDIYIIDIYKIILMHCSVIF MHTILYNMLFKRDCHF*DTVTKK*GNYFASIYS LCQKSKDYKCFAKMVSRLLKCFITVTFKIVTLNK EESSTVIRCMINQNMKMGNV*LT**LGGLSTWV NFICDFCFVLFCSD*LLIALESLLQN*KKNRCA FSF*EWNHPKEFLFLRFGCCS**RIFLLCSAVLK IAEVGARSLVVIVWNGRSESSVIELSILPSLLQ VFMLERDAF*YKTDVLILLIVLYIY*SLRLLHY GLEPIPM*FQSC*ESNGDFTCYCS*LHYRILLIF LVKM*FFISYIY*IFIFY*QLNTISVYRLVLLD FTNEFFKIQKKVVFPS*HTQF*ITCSVT*IFVL LTK*FIFY*FNITV*ECQSLLVLV*FSLKEQRSF IWISYKYIIIAK*EVKLLLSQQSWQTIE*YFDD LFCL*LVIIRRSRS*ILE*NLFSTVSISNVKIL FNIFEIPEYQALL*ISCIINK*NKGLFVDNPNTQ SLRNENYSVCANS*GLIMLQFSMLERLGRRLTF CTSVYSQKRETCIIVRSYFE*KRL*LQGVLLRL IQ*QTEQNCLQKWHRVSTPYPFKHVALLSCGQLV VCQDFFSWKDTPSFQTLMTDKNSMGPNLPEDHY QK*QVLLPLR*NHGSDIPYIFQNYCMFKTSTKK ERKNYTKNIYYSDQFLPISVVYCSQKNLQNKY*L SQNLNHKQANQTAHCSYSCYVIVF*LL*DPVLS AGEK*NAVQISWF*FSTQKHSKMQNVIMGTCLK ELVYPAFTPAG*KCCTYQQPYHFHLLKGQMCLVL LLCNHNLLSACSCLKLCILSWAAICFMLILLLL Q*LTLLYGKIK*NCPNKTSLS*V

Frame 1

YIL*HPVNYDMGLRFICSGDNMAYLPWNIPVFQ K*HIIFKEGALLSLNHMVQSWEYSGL*ITNIIY KLEGKLTCVCACVTVSLRNSKTVDSLLLEKNTRW QSCRCFSRLLALQVQKLFGAF*GGSLTRSQDSS LIGF*PSI*PCRLPCKARCKGLFQNEPTGVPSS R*KKREGVRSARPPRGYNAERMRWKIPTGGRGDP RRPRGLRLPPALGAAGRPPMTRSCGLCPVDRRP CGPGKRGRAPAES*RAFFSPPRALGARRVRPGL PGGAGRAGEGPRSSSGSQTSAIRGCTATCNSLFT AAE*VVAPTMLP*QGADKFHFPSRASWNHDSHN ALGTWSTVGPPLKKKCERLVLRSAFPDVSAAWN RF*SSLCFCLACCQLDCDDSTSTLHL*PRHPHFY L*SREKL*VSGIALGCFSVKRKVC*KSKISSAR KSRRKKKIIFSILL*TAASVYAKLINTDCTTKL HCKFSCEEIPFQTTLLKAAWKFGVEGSATFSCLH FGLQIGTGKGLLRAQG*YQALLLNVHLLTILVF L*KPKPL*INSFML*ISSGRSFP*Y*RHNFPCT FKAGSGEKYFEGTFSSSVQLFFFGCCSFLGGLGL LVSLKHLTTCKI*TWLLVSHQFGTQNG*HWKYV ESSRGGLAHGQTRNVNLHILRKAAKLKMDE*SP ALIH*KAVAPGRTANIFIHPHI*KRSWR*MDAIT *FSRRTWPCWPSKCN*PMP*CLVPHYNPCQCFQ LHQA*PPMHQQPPLIPIWDLFLQAWSRQRSCRL HQCWLQGIRVSLYLQLLQLLHRN*CEQTDLRYVE STNVAIATEEKMIVGLLILLTAQ*LTPMTTQSL CVWITSKGDALGKSANTFIPLHICKPRSRLPNT RSTRLQLHRLQPPQLPWEFLKLYFPHYQRGLLLK KPTVPPQSLTLVFSNTNRL*PTCSYNSIQHFSH QFPWCTVLRQPLCPQQQHLPQVFPSLQQPQPTR YP*YLPNI*LATSMLPRCRIFITKQSC*RGKDSV LG*SKGRGH*PYCIYRQATHTKVPQPQDIHILH VNQKKRQHFPEIHCTLLPIHFVHLIDICAEVIF LSKRTTLSFFSSTELCIQRCIPS*FPIYSCHLEK TDYGVTMILLCIGTSVDQDIIF*K*VYFFQGLQ ITKVHLVFKIAIIDESVHAQSFLFKSNIFNVID CKTW*GRDLTEMNVPSKTTTVYILKHIMALSTML LRILMKCHRLYIKLEYYFFSVIVFRATFCCIFA STNQSKGTILFFFF*NQSCLRNGQFNFTVTIDS TTQTLSIQINSHILEIYI**IYIKLF*CIVV*YL CILYCITCYSKGIAISETQ*QKNEEIILLLFIA SVKSQKTINALQKWFHVCLNASSQSHSK**L*T KKKAALSSDA**TKI*KWEMFN*PSNWVG*VHG* ILYVIFVLFCFVQINCL*P*KAFYKIKKKIDVH SVFKNGIIQRNSFF*GLDVAASKGYFCSVQQF* KLLK*GPGHW*L*YGMGEVKVQL*NFPYFQVYCK FLCLREMLSNIRLMC*FY*LYCTSIKALDYYIT GWNPYQCNFNRVKKVMVTSHVIVVSYIIEYYLF FLLKCSFSFPTFIRFSFSINN*IPFQFIDLFY*I LPMNFSKYKKK*FFLHNILSFELHVVSHEYSYC *LNDLYFTDLILQCKNVSHC*FLSSFH*KNKDL LYGYLINI*SLLSKKLSCCYRNNPGRQLSNILMI YFVCN*LL*EDLDPRY*NKIYFLLYPFQMLKYC LIFLKSLSIRPCYK*AA*SINRTRDFLLIIQIL KVYVMKIIACVQTLEG*LCCNLACWNV*GEG*LF ALLYIVKREKPV***DLILNKNVYNYKEFC*G* YNDRLSKIACKSGTELALHTPSNMLLCFLVDSL *FARIFSAGKIRHPFKPS*LTKTPWGQICLKIIT KNSRYFYH*GEIMDQIFLTFFKTTACLKLQQKK RERTILRTYIIQISFCQFQWFIVHKKIFKTSID FHKI*IINRQTKQHTVAIVVM*LFFNCCRILFFQ QVKNKTQFKFHGFNFQLRSTQKCKM**WALV*K N*CIQPSLQLVKNVALISNPTTFIC*KDKCAWF YYYVITTYFLLVVA*NYVFCLGLQFVLCLFYYYC SS*LCCMEK*SEIALIKLLFLK

Frame 2

TSYSTQ*IMIWGCGLFALATIWLTCRGISRYFR SNILFLKRELCFL*ITWCKVGNTVACKLQILFT N*KES*HVFVPV*QFH*GIPKLWIACYLRKIHG GSLADVFPAC*PSRFKNCLVHFKAAVSPDRRILV *LASNLQSNLAGFLAKPGARASFKMNPLVCLAV GRRSGRASGLHARREVTMLNA*DGRYQREAEGI HGARAGSGFLLLSAPLGDRP*PALAGSVRLTGDP VARGSAGGRRRKVEERFSRRRVH*ELDESALGF LVGLGGRGRGRAAAAEARPRR*EAAQRHATVFS LQLNELWRPQCSHDKELTSSIFRRGHLGIMTPTM PWALGRQWGRL*KKNVRGWY*EVPFLTSLLLGT ASRAVSAFALLAAS*TVTTAHPPSTSSPDTPIS TYNQEKSSKYLALP*AALVLKEKFAEKVRYLLPG NQGGKKKSFSRFCSKLLHLSMPN*SIPIAPPNS IANSAVRRFPFRQLC*KQLGNSVSKGLPRFHAC ILGSKLALGRGY*EHKADTRPYF*TFIYLQS*YF SKNQNLFELTVSCCEFLVGDLFLDIDDTIFHVL LKQGVGKSILRGHFHHQFSFFFLVVALFWGGWV CWFH*NI*LPVKSKHGC*CHTNSGHKMANTGSM* RVPEGDLLTARHGM*ICTSFEKLPS*KWTSNRL L*FIERPLLQGELQISSSTPTFKNAVGDKWTQ* LDSAEEHGHVGPANATSQCHDAWCPITTRANVFS CTKLSHQCISSRL*SLSGTCFSKPGPGRDLADC TNVGYRESGCPCTCSCCSCCTEINANRQT*GMS RVPTWQLQPRRK*LSVCSSC*QHND*HQ*QHSHC VYGLHQREMLSGKVQILSSPCTFASQDQGCPIP GQPGCSCTGCSHRSCHGNSSSCTSPITKEACS* KNQRCHRSL*HWYFPIPTGSSQHAVTTAYSISPT SSHGARCYASHCVRSNNICHKCSLRCNSHSQPD THNICRTSD*PQVCYPDVEFSSLNNHAKEERTV CLVRVKDEVISHIVYIVKQHTQKFLSHKTSTYCM LTRRKDNIFRKSTAHCCLYTLYI*LIFVLR*YS CLKEQHCLSFLAQSYAFKDAYLVSFLYIHAILK RQTMV*P*FYYVLVRL*TKI*FFKNKFISFKVYK *QRCTLYLKLPL*MRACMHSHFCLRVIFLM**I VRRGEGGI*QR*MCQAKPQLCIF*STSWL*VPC C*GFS*SAIDCTSN*SIISSVLLFSEPHFVAYLL VLISQRAPFFFFFFETKAVSEMANLTLQ*Q*TA QHKLSQYR*THTYWRYIYNRYI*NYFNAL*CNI YAYYTV*HVIQKGLPFLRHSNKKMRKLFCFYL*P LSKVKRL*MLCKNGFTFA*MLHHSHIQNSDSKQ RRKQHCHQMHDKPKYENGKCLINLVIGWVKYMG EFYM*FLFCFVLFRLTAYSLRKPFTKLKKK*MCI QFLRMESSKGIPFFEVWMLQLVKDIFALFSSSK NC*SRGQVTGSYSMEWEK*KFSYRTFHTSKFTA SFYA*ERCFLI*D*CVDFTDCTVHLLKP*IITLR VGTHTNVISIVLRK*W*LHMLL*LVTL*NITYF SC*NVVFHFLHLLDFHFLLTIEYHFSL*TCFIR FYQ*IFQNTKKSSFSFITYSVLNYM*CHMNIRIV N*MIYILLI*YYSVRMSVIVSSCLVFIKRTKIF YMDIL*IYNHC*VRS*VVAIATILADN*VIF** FILFVISYYKKI*ILDIRIKFIFYCIHFKC*NIV *YF*NP*VSGLVINKLHNQ*IEQGTFC**SKYS KFT**KL*RVCKLLRVDYAAI*HVGTSREKVDF LHFCI*SKERNLYNSKILF*IKTSIITRSFVKAN TMTD*AKLLAKVAQS*HSIPLQTCCFAFLWTAC SLPGFFQLERYAILSNPHD*QKLHGAKSA*RSL PKIAGTSTIKVKSWIRYSLHFSKLLHV*NFNKKR EKELY*EHILFRSVSANFSGLLFTKKSSKQVLT FTKFKS*TGKPNSTL*L*LLCDCFLIAVGSCSF SR*KIKRSSNFMVLIFNSEALKNAKCDNGHLFKR ISVSSLHSSWLKMLHLSATLPLSSAERTNVLGF TIM*SQLTFCL*LLKIMYFVLGCNLFYAYFIIT AVVDFAVWKNKVKLP**NFSFLS

Sequence NM_207294

GTTGGTACTAAGAAGTGCCTTTCCTGACGTCTCTGCTGCTTGGAACCGCTTCTAGAGCAGTCTCTGCTTTTGCCTTGCTTGCTGCCAGCTAGACTGTGAC GACAGCACATCCACCCTCCACCTCTAGCCCAGACACCCCCATTTCTACTTATAATCAAGAGAAAAGCTCTAAGTATCTGGCATTGCCCTAGGCTGCTTTA GTGTTAAAAGAAAAGTTTGCTGAAAAAGTAAGATATCTTCTGCCAGGAAATCAAGGAGGAAAAAAAAAATCATTTTCTCGATTTTGCTCTAAACTGCTGC ATCTGTCTATGCCAAACTAATCAATACCGATTGCACCACCAAACTCCATTGCAAATTCAGCTGTGAGGAGATTCCCTTTCAGACAACTTTGCTGAAAGCA GCTTGGAAATTCGGTGTCGAAGGGTCTGCCACGTTTTCATGCTTGCATTTTGGGCTCCAAATTGGCACTGGGAAGGGGTTACTGAGAGCACAAGGCTGAT ACCAGGCCCTACTTTTAAACGTTCATCTACTTACAATCCTAGTATTTCTCTAAAAACCAAAACCTCTTTGAATTAACAGTTTCATGCTGTGAATTTCTAG TGGGAGATCTTTTCCTTGATATTGACGACACAATTTTCCATGTACTTTTAAAGCAGGGAGTGGGGAAAAGTATTTTGAGGGGACATTTTCATCATCAGTT CAGCTTTTTTTTTTTGGTTGTTGCTCTTTTTTGGGGGGGTTGGGTTTGTTGGTTTCACTGAAACATTTAACTACCTGTAAAATCTAAACATGGCTGTTAG TGTCACACCAATTCGGGACACAAAATGGCTAACACTGGAAGTATGTAGAGAGTTCCAGAGGGGGACTTGCTCACGGCCAGACACGGAATGTAAATTTGCA CATCCTTCGAAAAGCTGCCAAGTTGAAAATGGACGAGTAATCGCCTGCTTTGATTCATTGAAAGGCCGTTGCTCCAGGGAGAACTGCAAATATCTTCATC CACCCCCACATTTAAAAACGCAGTTGGAGATAAATGGACGCAATAACTTGATTCAGCAGAAGAACATGGCCATGTTGGCCCAGCAAATGCAACTAGCCAA TGCCATGATGCCTGGTGCCCCATTACAACCCGTGGTATGTCGAGAGTACCAACGTGGCAATTGCAACCGAGGAGAAAATGATTGTCGGTTTGCTCATCCT GCTGACAGCACAATGATTGACACCAATGACAACACAGTCACTGTGTGTATGGATTACATCAAAGGGAGATGCTCTCGGGAAAAGTGCAAATACTTTCATC CCCCTGCACATTTGCAAGCCAAGATCAAGGCTGCCCAATACCAGGTCAACCAGGCTGCAGCTGCACAGGCTGCAGCCACCGCAGCTGCCATGGGAATTCC TCAAGCTGTACTTCCCCCATTACCAAAGAGGCCTGCTCTTGAAAAAACCAACGGTGCCACCGCAGTCTTTAACACTGGTATTTTCCAATACCAACAGGCT CTAGCCAACATGCAGTTACAACAGCATACAGCATTTCTCCCACCAGTTCCCATGGTGCACGGTGCTACGCCAGCCACTGTGTCCGCAGCAACAACATCTG CCACAAGTGTTCCCTTCGCTGCAACAGCCACAGCCAACCAGATACCCATAATATCTGCCGAACATCTGACTAGCCACAAGTATGTTACCCAGATGTAGAA TTTTCATCACTAAACAATCATGCTAAAGAGGAAAGGACAGTGTGCTTGGTTAGAGTAAAGGACGAGGTCATTAGCCATATTGTATATATCGTCAAGCAAC ACACACAAAAGTTCCTCAGCCACAAGACATCCACATATTGCATGTTAACCAGAAGAAAAGACAACATTTTCCGGAAATCCACTGCACACTGTTGCCTATA CACTTTGTACATTTAATTGATATTTGTGCTGAGGTGATATTCCTGTCTAAAAGAACAACATTGTCTTTCTTTTCTAGCACAGAGTTATGCATTCAAAGAT GCATACCTAGTTAGTTTCCTATATATTCATGCCATCTTGAAAAGACAGACTATGGTGTAACCATGATTCTATTATGTATTGGTACGTCTGTAGACCAAGA TATAATTTTTTAAAAATAAGTTTATTTCTTTCAAGGTTTACAAATAACAAAGGTGCACCTTGTATTTAAAATTGCCATTATAGATGAGAGCGTGCATGCA CAGTCATTTTTGTTTAAGAGTAATATTTTTAATGTAATAGATTGTAAGACGTGGTGAGGGAGGGATCTGACAGAGATGAATGTGCCAAGCAAAACCACAA CTGTGTATATTTTAAAGCACATCATGGCTTTAAGTACCATGTTGTTAAGGATTCTCATGAAGTGCCATAGACTGTACATCAAATTAGAGTATTATTTCTT CAGTGTTATTGTTTTCAGAGCCACATTTTGTTGCATATTTGCTAGTACTAATCAGTCAAAGGGCACCATTCTTTTTTTTTTTTTTTGAAACCAAAGCTGT CTCAGAAATGGCCAATTTAACTTTACAGTAACAATAGACAGCACAACACAAACTCTCTCAATACAGATAAACTCACACATACTGGAGATATATATATAAT AGATATATATAAAATTATTTTAATGCATTGTAGTGTAATATTTATGCATACTATACTGTATAACATGTTATTCAAAAGGGATTGCCATTTCTGAGACACA GTAACAAAAAAATGAGGAAATTATTTTGCTTCTATTTATAGCCTCTGTCAAAAGTCAAAAGACTATAAATGCTTTGCAAAAATGGTTTCACGTTTGCTTA AATGCTTCATCACAGTCACATTCAAAATAGTGACTCTAAACAAAGAAGAAAGCAGCACTGTCATCAGATGCATGATAAACCAAAATATGAAAATGGGAAA TGTTTAATTAACCTAGTAATTGGGTGGGTTAAGTACATGGGTGAATTTTATATGTGATTTTTGTTTTGTTTTGTTTTGTTCAGATTAACTGCTTATAGCC TTAGAAAGCCTTTTACAAAATTAAAAAAAAAATAGATGTGCATTCAGTTTTTAAGAATGGAATCATCCAAAGGAATTCCTTTTTTTGAGGTTTGGATGTT GCAGCTAGTAAAGGATATTTTTGCTCTGTTCAGCAGTTCTAAAAATTGCTGAAGTAGGGGCCAGGTCACTGGTAGTTATAGTATGGAATGGGAGAAGTGA AAGTTCAGTTATAGAACTTTCCATACTTCCAAGTTTACTGCAAGTTTTTATGCTTGAGAGAGATGCTTTCTAATATAAGACTGATGTGTTGATTTTACTG ATTGTACTGTACATCTATTAAAGCCTTAGATTATTACATTACGGGTTGGAACCCATACCAATGTAATTTCAATCGTGTTAAGAAAGTAATGGTGACTTCA CATGTTATTGTAGTTAGTTACATTATAGAATATTACTTATTTTTCTTGTTAAAATGTAGTTTTTCATTTCCTACATTTATTAGATTTTCATTTTCTATTA ACAATTGAATACCATTTCAGTTTATAGACTTGTTTTATTAGATTTTACCAATGAATTTTTCAAAATACAAAAAAAAGTAGTTTTTCCTTCATAACATACT CAGTTTTGAATTACATGTAGTGTCACATGAATATTCGTATTGTTAACTAAATGATTTATATTTTACTGATTTAATATTACAGTGTAAGAATGTCAGTCAT TGTTAGTTCTTGTCTAGTTTTCATTAAAAGAACAAAGATCTTTTATATGGATATCTTATAAATATATAATCATTGCTAAGTAAGAAGTTAAGTTGTTGCT ATCGCAACAATCCTGGCAGACAATTGAGTAATATTTTGATGATTTATTTTGTTTGTAATTAGTTATTATAAGAAGATCTAGATCCTAGATATTAGAATAA AATTTATTTTCTACTGTATCCATTTCAAATGTTAAAATATTGTTTAATATTTTTGAAATCCCTGAGTATCAGGCCTTGTTATAAATAAGCTGCATAATCA ATAAATAGAACAAGGGACTTTTTGTTGATAATCCAAATACTCAAAGTTTACGTAATGAAAATTATAGCGTGTGTGCAAACTCTTGAGGGTTGATTATGCT GCAATTTAGCATGTTGGAACGTCTAGGGAGAAGGTTGACTTTTTGCACTTCTGTATATAGTCAAAAGAGAGAAACCTGTATAATAGTAAGATCTTATTTT GAATAAAAACGTCTATAATTACAAGGAGTTTTGTTAAGGCTAATACAATGACAGACTGAGCAAAATTGCTTGCAAAAGTGGCACAGAGTTAGCACTCCAT ACCCCTTCAAACATGTTGCTTTGCTTTCTTGTGGACAGCTTGTAGTTTGCCAGGATTTTTTCAGCTGGAAAGATACGCCATCCTTTCAAACCCTCATGAC TGACAAAAACTCCATGGGGCCAAATCTGCCTGAAGATCATTACCAAAAATAGCAGGTACTTCTACCATTAAGGTGAAATCATGGATCAGATATTCCTTAC ATTTTTCAAAACTACTGCATGTTTAAAACTTCAACAAAAAAAGAGAGAAAGAACTATACTAAGAACATATATTATTCAGATCAGTTTCTGCCAATTTCAG TGGTTTATTGTTCACAAAAAAATCTTCAAAACAAGTATTGACTTTCACAAAATTTAAATCATAAACAGGCAAACCAAACAGCACACTGTAGCTATAGTTG TTATGTGATTGTTTTTTAATTGCTGTAGGATCCTGTTCTTTCAGCAGGTGAAAAATAAAACGCAGTTCAAATTTCATGGTTTTAATTTTCAACTCAGAAG CACTCAAAAATGCAAAATGTGATAATGGGCACTTGTTTAAAAGAATTAGTGTATCCAGCCTTCACTCCAGCTGGTTAAAAATGTTGCACTTATCAGCAAC CCTACCACTTTCATCTGCTGAAAGGACAAATGTGCTTGGTTTTACTATTATGTAATCACAACTTACTTTCTGCTTGTAGTTGCTTAAAATTATGTATTTT GTCTTGGGCTGCAATTTGTTTTATGCTTATTTTATTATTACTGCAGTAGTTGACTTTGCTGTATGGAAAAATAAAGTGAAATTGCCCTAATAAAACTTCT CTTTCTTAAGTA

refseq_MBNL1.F3 (forward primer)
refseq_MBNL1.R3 (reverse primer)
Single exon skipping

Frame 0

VGTKKCLS*RLCCLEPLLEQSLLLPCLLPARL* RQHIHPPPLAQTPPFLLIIKRKALSIWHCPRLL* C*KKSLLKK*DIFCQEIKEEKKNHFLDFALNCC ICLCQTNQYRLHHQTPLQIQL*GDSLSDNFAES SLEIRCRRVCHVFMLAFWAPNWHWEGVTESTRLI PGPTFKRSSTYNPSISLKTKTSLN*QFHAVNF* WEIFSLILTTQFSMYF*SREWGKVF*GDIFIIS SAFFFWLLLFFGGVGFVGFTETFNYL*NLNMAVS VTPIRDTKWLTLEVCREFQRGTCSRPDTECKFA HPSKSCQVENGRVIACFDSLKGRCSRENCKYLH PPPHLKTQLEINGRNNLIQQKNMAMLAQQMQLAN AMMPGAPLQPVVCREYQRGNCNRGENDCRFAHP ADSTMIDTNDNTVTVCMDYIKGRCSREKCKYFH PPAHLQAKIKAAQYQVNQAAAAQAAATAAAMGIP QAVLPPLPKRPALEKTNGATAVFNTGIFQYQQA LANMQLQQHTAFLPPVPMVHGATPATVSAATTS ATSVPFAATATANQIPIISAEHLTSHKYVTQM*N FHH*TIMLKRKGQCAWLE*RTRSLAILYISSSN THKSSSATRHPHIAC*PEEKTTFSGNPLHTVAY TLCTFN*YLC*GDIPV*KNNIVFLF*HRVMHSKM HT*LVSYIFMPS*KDRLWCNHDSIMYWYVCRPR YNFLKISLFLSRFTNNKGAPCI*NCHYR*ERAC TVIFV*E*YF*CNRL*DVVREGSDRDECAKQNHN CVYFKAHHGFKYHVVKDSHEVP*TVHQIRVLFL QCYCFQSHILLHIC*Y*SVKGHHSFFFFLKPKL SQKWPI*LYSNNRQHNTNSLNTDKLTHTGDIYII DIYKIILMHCSVIFMHTILYNMLFKRDCHF*DT VTKK*GNYFASIYSLCQKSKDYKCFAKMVSRLL KCFITVTFKIVTLNKEESSTVIRCMINQNMKMGN V*LT**LGGLSTWVNFICDFCFVLFCSD*LLIA LESLLQN*KKNRCAFSF*EWNHPKEFLFLRFGC CS**RIFLLCSAVLKIAEVGARSLVVIVWNGRSE SSVIELSILPSLLQVFMLERDAF*YKTDVLILL IVLYIY*SLRLLHYGLEPIPM*FQSC*ESNGDF TCYCS*LHYRILLIFLVKM*FFISYIY*IFIFY* QLNTISVYRLVLLDFTNEFFKIQKKVVFPS*HT QF*ITCSVT*IFVLLTK*FIFY*FNITV*ECQS LLVLV*FSLKEQRSFIWISYKYIIIAK*EVKLLL SQQSWQTIE*YFDDLFCL*LVIIRRSRS*ILE* NLFSTVSISNVKILFNIFEIPEYQALL*ISCII NK*NKGLFVDNPNTQSLRNENYSVCANS*GLIML QFSMLERLGRRLTFCTSVYSQKRETCIIVRSYF E*KRL*LQGVLLRLIQ*QTEQNCLQKWHRVSTP YPFKHVALLSCGQLVVCQDFFSWKDTPSFQTLMT DKNSMGPNLPEDHYQK*QVLLPLR*NHGSDIPY IFQNYCMFKTSTKKERKNYTKNIYYSDQFLPIS VVYCSQKNLQNKY*LSQNLNHKQANQTAHCSYSC YVIVF*LL*DPVLSAGEK*NAVQISWF*FSTQK HSKMQNVIMGTCLKELVYPAFTPAG*KCCTYQQ PYHFHLLKGQMCLVLLLCNHNLLSACSCLKLCIL SWAAICFMLILLLLQ*LTLLYGKIK*NCPNKTS LS*V

Frame 1

LVLRSAFPDVSAAWNRF*SSLCFCLACCQLDCD DSTSTLHL*PRHPHFYL*SREKL*VSGIALGCF SVKRKVC*KSKISSARKSRRKKKIIFSILL*TAA SVYAKLINTDCTTKLHCKFSCEEIPFQTTLLKA AWKFGVEGSATFSCLHFGLQIGTGKGLLRAQG* YQALLLNVHLLTILVFL*KPKPL*INSFML*ISS GRSFP*Y*RHNFPCTFKAGSGEKYFEGTFSSSV QLFFFGCCSFLGGLGLLVSLKHLTTCKI*TWLL VSHQFGTQNG*HWKYVESSRGGLAHGQTRNVNLH ILRKAAKLKMDE*SPALIH*KAVAPGRTANIFI HPHI*KRSWR*MDAIT*FSRRTWPCWPSKCN*P MP*CLVPHYNPWYVESTNVAIATEEKMIVGLLIL LTAQ*LTPMTTQSLCVWITSKGDALGKSANTFI PLHICKPRSRLPNTRSTRLQLHRLQPPQLPWEF LKLYFPHYQRGLLLKKPTVPPQSLTLVFSNTNRL *PTCSYNSIQHFSHQFPWCTVLRQPLCPQQQHL PQVFPSLQQPQPTRYP*YLPNI*LATSMLPRCR IFITKQSC*RGKDSVLG*SKGRGH*PYCIYRQAT HTKVPQPQDIHILHVNQKKRQHFPEIHCTLLPI HFVHLIDICAEVIFLSKRTTLSFFSSTELCIQR CIPS*FPIYSCHLEKTDYGVTMILLCIGTSVDQD IIF*K*VYFFQGLQITKVHLVFKIAIIDESVHA QSFLFKSNIFNVIDCKTW*GRDLTEMNVPSKTT TVYILKHIMALSTMLLRILMKCHRLYIKLEYYFF SVIVFRATFCCIFASTNQSKGTILFFFF*NQSC LRNGQFNFTVTIDSTTQTLSIQINSHILEIYI* *IYIKLF*CIVV*YLCILYCITCYSKGIAISETQ *QKNEEIILLLFIASVKSQKTINALQKWFHVCL NASSQSHSK**L*TKKKAALSSDA**TKI*KWE MFN*PSNWVG*VHG*ILYVIFVLFCFVQINCL*P *KAFYKIKKKIDVHSVFKNGIIQRNSFF*GLDV AASKGYFCSVQQF*KLLK*GPGHW*L*YGMGEV KVQL*NFPYFQVYCKFLCLREMLSNIRLMC*FY* LYCTSIKALDYYITGWNPYQCNFNRVKKVMVTS HVIVVSYIIEYYLFFLLKCSFSFPTFIRFSFSI NN*IPFQFIDLFY*ILPMNFSKYKKK*FFLHNIL SFELHVVSHEYSYC*LNDLYFTDLILQCKNVSH C*FLSSFH*KNKDLLYGYLINI*SLLSKKLSCC YRNNPGRQLSNILMIYFVCN*LL*EDLDPRY*NK IYFLLYPFQMLKYCLIFLKSLSIRPCYK*AA*S INRTRDFLLIIQILKVYVMKIIACVQTLEG*LC CNLACWNV*GEG*LFALLYIVKREKPV***DLIL NKNVYNYKEFC*G*YNDRLSKIACKSGTELALH TPSNMLLCFLVDSL*FARIFSAGKIRHPFKPS* LTKTPWGQICLKIITKNSRYFYH*GEIMDQIFLT FFKTTACLKLQQKKRERTILRTYIIQISFCQFQ WFIVHKKIFKTSIDFHKI*IINRQTKQHTVAIV VM*LFFNCCRILFFQQVKNKTQFKFHGFNFQLRS TQKCKM**WALV*KN*CIQPSLQLVKNVALISN PTTFIC*KDKCAWFYYYVITTYFLLVVA*NYVF CLGLQFVLCLFYYYCSS*LCCMEK*SEIALIKLL FLK

Frame 2

WY*EVPFLTSLLLGTASRAVSAFALLAAS*TVT TAHPPSTSSPDTPISTYNQEKSSKYLALP*AAL VLKEKFAEKVRYLLPGNQGGKKKSFSRFCSKLL HLSMPN*SIPIAPPNSIANSAVRRFPFRQLC*KQ LGNSVSKGLPRFHACILGSKLALGRGY*EHKAD TRPYF*TFIYLQS*YFSKNQNLFELTVSCCEFL VGDLFLDIDDTIFHVLLKQGVGKSILRGHFHHQF SFFFLVVALFWGGWVCWFH*NI*LPVKSKHGC* CHTNSGHKMANTGSM*RVPEGDLLTARHGM*IC TSFEKLPS*KWTSNRLL*FIERPLLQGELQISSS TPTFKNAVGDKWTQ*LDSAEEHGHVGPANATSQ CHDAWCPITTRGMSRVPTWQLQPRRK*LSVCSS C*QHND*HQ*QHSHCVYGLHQREMLSGKVQILSS PCTFASQDQGCPIPGQPGCSCTGCSHRSCHGNS SSCTSPITKEACS*KNQRCHRSL*HWYFPIPTG SSQHAVTTAYSISPTSSHGARCYASHCVRSNNIC HKCSLRCNSHSQPDTHNICRTSD*PQVCYPDVE FSSLNNHAKEERTVCLVRVKDEVISHIVYIVKQ HTQKFLSHKTSTYCMLTRRKDNIFRKSTAHCCLY TLYI*LIFVLR*YSCLKEQHCLSFLAQSYAFKD AYLVSFLYIHAILKRQTMV*P*FYYVLVRL*TK I*FFKNKFISFKVYK*QRCTLYLKLPL*MRACMH SHFCLRVIFLM**IVRRGEGGI*QR*MCQAKPQ LCIF*STSWL*VPCC*GFS*SAIDCTSN*SIIS SVLLFSEPHFVAYLLVLISQRAPFFFFFFETKAV SEMANLTLQ*Q*TAQHKLSQYR*THTYWRYIYN RYI*NYFNAL*CNIYAYYTV*HVIQKGLPFLRH SNKKMRKLFCFYL*PLSKVKRL*MLCKNGFTFA* MLHHSHIQNSDSKQRRKQHCHQMHDKPKYENGK CLINLVIGWVKYMGEFYM*FLFCFVLFRLTAYS LRKPFTKLKKK*MCIQFLRMESSKGIPFFEVWML QLVKDIFALFSSSKNC*SRGQVTGSYSMEWEK* KFSYRTFHTSKFTASFYA*ERCFLI*D*CVDFT DCTVHLLKP*IITLRVGTHTNVISIVLRK*W*LH MLL*LVTL*NITYFSC*NVVFHFLHLLDFHFLL TIEYHFSL*TCFIRFYQ*IFQNTKKSSFSFITY SVLNYM*CHMNIRIVN*MIYILLI*YYSVRMSVI VSSCLVFIKRTKIFYMDIL*IYNHC*VRS*VVA IATILADN*VIF**FILFVISYYKKI*ILDIRI KFIFYCIHFKC*NIV*YF*NP*VSGLVINKLHNQ *IEQGTFC**SKYSKFT**KL*RVCKLLRVDYA AI*HVGTSREKVDFLHFCI*SKERNLYNSKILF *IKTSIITRSFVKANTMTD*AKLLAKVAQS*HSI PLQTCCFAFLWTACSLPGFFQLERYAILSNPHD *QKLHGAKSA*RSLPKIAGTSTIKVKSWIRYSL HFSKLLHV*NFNKKREKELY*EHILFRSVSANFS GLLFTKKSSKQVLTFTKFKS*TGKPNSTL*L*L LCDCFLIAVGSCSFSR*KIKRSSNFMVLIFNSE ALKNAKCDNGHLFKRISVSSLHSSWLKMLHLSAT LPLSSAERTNVLGFTIM*SQLTFCL*LLKIMYF VLGCNLFYAYFIITAVVDFAVWKNKVKLP**NF SFLS

Sequence NM_021038

TTACATCTTATAGCACCCAGTGAATTATGATATGGGGCTGCGGTTTATTTGCTCTGGCGACAATATGGCTTACTTGCCGTGGAATATCCCGGTATTTCAG AAGTAACATATTATTTTTAAAGAGGGAGCTTTGCTTTCTCTAAATCACATGGTGCAAAGTTGGGAATACAGTGGCCTGTAAATTACAAATATTATTTACA AACTAGAAGGAAAGCTGACATGTGTTTGTGCCTGTGTGACAGTTTCACTAAGGAATTCCAAAACTGTGGATAGCCTGTTACTTGAGAAAAATACACGGTG GCAGTCTTGCAGATGTTTTTCCCGCCTGTTAGCCCTCCAGGTTCAAAAACTGTTTGGTGCATTTTAAGGCGGCAGTCTCACCAGATCGCAGGATTCTAGT CTAATTGGCTTCTAACCTTCAATCTAACCTTGCCGGCTTCCTTGCAAAGCCCGGTGCAAGGGCCTCTTTCAAAATGAACCCACTGGTGTGCCTAGCAGTC GGTAGAAGAAGCGGGAGGGCGTCCGGTCTGCACGCCCGCCGCGAGGTTACAATGCTGAACGCATGAGATGGAAGATACCAACGGGAGGCCGAGGGGATCC ACGGCGCCCGCGCGGGCTCCGGCTTCCTCCTGCTCTCGGCGCCGCTGGGCGACCGCCCATGACCCGCTCTTGCGGGCTCTGTCCGGTTGACAGGCGACCC TGTGGCCCGGGGAAGCGCGGGAGGGCGCCGGCGGAAAGTTGAAGAGCGTTTTTCTCGCCGCCGCGTGCATTAGGAGCTCGACGAGTCCGCCCTGGGCTTC CTGGTGGGGCTGGGCGGGCGGGGGAGGGGCCGCGCAGCAGCAGCGGAAGCCAGACCTCGGCGATAAGAGGCTGCACAGCGACATGCAACAGTCTTTTCAC TGCAGCTGAATGAGTTGTGGCGCCCACAATGCTCCCATGACAAGGAGCTGACAAGTTCCATTTTCCGTCGCGGGCATCTTGGAATCATGACTCCCACAAT GCCTTGGGCACTTGGTCGACAGTGGGGCCGCCTCTGAAAAAAAAATGTGAGAGGTTGGTACTAAGAAGTGCCTTTCCTGACGTCTCTGCTGCTTGGAACC GCTTCTAGAGCAGTCTCTGCTTTTGCCTTGCTTGCTGCCAGCTAGACTGTGACGACAGCACATCCACCCTCCACCTCTAGCCCAGACACCCCCATTTCTA CTTATAATCAAGAGAAAAGCTCTAAGTATCTGGCATTGCCCTAGGCTGCTTTAGTGTTAAAAGAAAAGTTTGCTGAAAAAGTAAGATATCTTCTGCCAGG AAATCAAGGAGGAAAAAAAAAATCATTTTCTCGATTTTGCTCTAAACTGCTGCATCTGTCTATGCCAAACTAATCAATACCGATTGCACCACCAAACTCC ATTGCAAATTCAGCTGTGAGGAGATTCCCTTTCAGACAACTTTGCTGAAAGCAGCTTGGAAATTCGGTGTCGAAGGGTCTGCCACGTTTTCATGCTTGCA TTTTGGGCTCCAAATTGGCACTGGGAAGGGGTTACTGAGAGCACAAGGCTGATACCAGGCCCTACTTTTAAACGTTCATCTACTTACAATCCTAGTATTT CTCTAAAAACCAAAACCTCTTTGAATTAACAGTTTCATGCTGTGAATTTCTAGTGGGAGATCTTTTCCTTGATATTGACGACACAATTTTCCATGTACTT TTAAAGCAGGGAGTGGGGAAAAGTATTTTGAGGGGACATTTTCATCATCAGTTCAGCTTTTTTTTTTTGGTTGTTGCTCTTTTTTGGGGGGGTTGGGTTT GTTGGTTTCACTGAAACATTTAACTACCTGTAAAATCTAAACATGGCTGTTAGTGTCACACCAATTCGGGACACAAAATGGCTAACACTGGAAGTATGTA GAGAGTTCCAGAGGGGGACTTGCTCACGGCCAGACACGGAATGTAAATTTGCACATCCTTCGAAAAGCTGCCAAGTTGAAAATGGACGAGTAATCGCCTG CTTTGATTCATTGAAAGGCCGTTGCTCCAGGGAGAACTGCAAATATCTTCATCCACCCCCACATTTAAAAACGCAGTTGGAGATAAATGGACGCAATAAC TTGATTCAGCAGAAGAACATGGCCATGTTGGCCCAGCAAATGCAACTAGCCAATGCCATGATGCCTGGTGCCCCATTACAACCCGTGCCAATGTTTTCAG TTGCACCAAGCTTAGCCACCAATGCATCAGCAGCCGCCTTTAATCCCTATCTGGGACCTGTTTCTCCAAGCCTGGTCCCGGCAGAGATCTTGCCGACTGC ACCAATGTTGGTTACAGGGAATCCGGGTGTCCCTGTACCTGCAGCTGCTGCAGCTGCTGCACAGAAATTAATGCGAACAGACAGACTTGAGGTATGTCGA GAGTACCAACGTGGCAATTGCAACCGAGGAGAAAATGATTGTCGGTTTGCTCATCCTGCTGACAGCACAATGATTGACACCAATGACAACACAGTCACTG TGTGTATGGATTACATCAAAGGGAGATGCTCTCGGGAAAAGTGCAAATACTTTCATCCCCCTGCACATTTGCAAGCCAAGATCAAGGCTGCCCAATACCA GGTCAACCAGGCTGCAGCTGCACAGGCTGCAGCCACCGCAGCTGCCATGGGAATTCCTCAAGCTGTACTTCCCCCATTACCAAAGAGGCCTGCTCTTGAA AAAACCAACGGTGCCACCGCAGTCTTTAACACTGGTATTTTCCAATACCAACAGGCTCTAGCCAACATGCAGTTACAACAGCATACAGCATTTCTCCCAC CAGGCTCAATATTGTGCATGACACCCGCTACAAGTGTTGTTCCCATGGTGCACGGTGCTACGCCAGCCACTGTGTCCGCAGCAACAACATCTGCCACAAG TGTTCCCTTCGCTGCAACAGCCACAGCCAACCAGATACCCATAATATCTGCCGAACATCTGACTAGCCACAAGTATGTTACCCAGATGTAGAATTTTCAT CACTAAACAATCATGCTAAAGAGGAAAGGACAGTGTGCTTGGTTAGAGTAAAGGACGAGGTCATTAGCCATATTGTATATATCGTCAAGCAACACACACA AAAGTTCCTCAGCCACAAGACATCCACATATTGCATGTTAACCAGAAGAAAAGACAACATTTTCCGGAAATCCACTGCACACTGTTGCCTATACACTTTG TACATTTAATTGATATTTGTGCTGAGGTGATATTCCTGTCTAAAAGAACAACATTGTCTTTCTTTTCTAGCACAGAGTTATGCATTCAAAGATGCATACC TAGTTAGTTTCCTATATATTCATGCCATCTTGAAAAGACAGACTATGGTGTAACCATGATTCTATTATGTATTGGTACGTCTGTAGACCAAGATATAATT TTTTAAAAATAAGTTTATTTCTTTCAAGGTTTACAAATAACAAAGGTGCACCTTGTATTTAAAATTGCCATTATAGATGAGAGCGTGCATGCACAGTCAT TTTTGTTTAAGAGTAATATTTTTAATGTAATAGATTGTAAGACGTGGTGAGGGAGGGATCTGACAGAGATGAATGTGCCAAGCAAAACCACAACTGTGTA TATTTTAAAGCACATCATGGCTTTAAGTACCATGTTGTTAAGGATTCTCATGAAGTGCCATAGACTGTACATCAAATTAGAGTATTATTTCTTCAGTGTT ATTGTTTTCAGAGCCACATTTTGTTGCATATTTGCTAGTACTAATCAGTCAAAGGGCACCATTCTTTTTTTTTTTTTTTGAAACCAAAGCTGTCTCAGAA ATGGCCAATTTAACTTTACAGTAACAATAGACAGCACAACACAAACTCTCTCAATACAGATAAACTCACACATACTGGAGATATATATATAATAGATATA TATAAAATTATTTTAATGCATTGTAGTGTAATATTTATGCATACTATACTGTATAACATGTTATTCAAAAGGGATTGCCATTTCTGAGACACAGTAACAA AAAAATGAGGAAATTATTTTGCTTCTATTTATAGCCTCTGTCAAAAGTCAAAAGACTATAAATGCTTTGCAAAAATGGTTTCACGTTTGCTTAAATGCTT CATCACAGTCACATTCAAAATAGTGACTCTAAACAAAGAAGAAAGCAGCACTGTCATCAGATGCATGATAAACCAAAATATGAAAATGGGAAATGTTTAA TTAACCTAGTAATTGGGTGGGTTAAGTACATGGGTGAATTTTATATGTGATTTTTGTTTTGTTTTGTTTTGTTCAGATTAACTGCTTATAGCCTTAGAAA GCCTTTTACAAAATTAAAAAAAAAATAGATGTGCATTCAGTTTTTAAGAATGGAATCATCCAAAGGAATTCCTTTTTTTGAGGTTTGGATGTTGCAGCTA GTAAAGGATATTTTTGCTCTGTTCAGCAGTTCTAAAAATTGCTGAAGTAGGGGCCAGGTCACTGGTAGTTATAGTATGGAATGGGAGAAGTGAAAGTTCA GTTATAGAACTTTCCATACTTCCAAGTTTACTGCAAGTTTTTATGCTTGAGAGAGATGCTTTCTAATATAAGACTGATGTGTTGATTTTACTGATTGTAC TGTACATCTATTAAAGCCTTAGATTATTACATTACGGGTTGGAACCCATACCAATGTAATTTCAATCGTGTTAAGAAAGTAATGGTGACTTCACATGTTA TTGTAGTTAGTTACATTATAGAATATTACTTATTTTTCTTGTTAAAATGTAGTTTTTCATTTCCTACATTTATTAGATTTTCATTTTCTATTAACAATTG AATACCATTTCAGTTTATAGACTTGTTTTATTAGATTTTACCAATGAATTTTTCAAAATACAAAAAAAAGTAGTTTTTCCTTCATAACATACTCAGTTTT GAATTACATGTAGTGTCACATGAATATTCGTATTGTTAACTAAATGATTTATATTTTACTGATTTAATATTACAGTGTAAGAATGTCAGTCATTGTTAGT TCTTGTCTAGTTTTCATTAAAAGAACAAAGATCTTTTATATGGATATCTTATAAATATATAATCATTGCTAAGTAAGAAGTTAAGTTGTTGCTATCGCAA CAATCCTGGCAGACAATTGAGTAATATTTTGATGATTTATTTTGTTTGTAATTAGTTATTATAAGAAGATCTAGATCCTAGATATTAGAATAAAATTTAT TTTCTACTGTATCCATTTCAAATGTTAAAATATTGTTTAATATTTTTGAAATCCCTGAGTATCAGGCCTTGTTATAAATAAGCTGCATAATCAATAAATA GAACAAGGGACTTTTTGTTGATAATCCAAATACTCAAAGTTTACGTAATGAAAATTATAGCGTGTGTGCAAACTCTTGAGGGTTGATTATGCTGCAATTT AGCATGTTGGAACGTCTAGGGAGAAGGTTGACTTTTTGCACTTCTGTATATAGTCAAAAGAGAGAAACCTGTATAATAGTAAGATCTTATTTTGAATAAA AACGTCTATAATTACAAGGAGTTTTGTTAAGGCTAATACAATGACAGACTGAGCAAAATTGCTTGCAAAAGTGGCACAGAGTTAGCACTCCATACCCCTT CAAACATGTTGCTTTGCTTTCTTGTGGACAGCTTGTAGTTTGCCAGGATTTTTTCAGCTGGAAAGATACGCCATCCTTTCAAACCCTCATGACTGACAAA AACTCCATGGGGCCAAATCTGCCTGAAGATCATTACCAAAAATAGCAGGTACTTCTACCATTAAGGTGAAATCATGGATCAGATATTCCTTACATTTTTC AAAACTACTGCATGTTTAAAACTTCAACAAAAAAAGAGAGAAAGAACTATACTAAGAACATATATTATTCAGATCAGTTTCTGCCAATTTCAGTGGTTTA TTGTTCACAAAAAAATCTTCAAAACAAGTATTGACTTTCACAAAATTTAAATCATAAACAGGCAAACCAAACAGCACACTGTAGCTATAGTTGTTATGTG ATTGTTTTTTAATTGCTGTAGGATCCTGTTCTTTCAGCAGGTGAAAAATAAAACGCAGTTCAAATTTCATGGTTTTAATTTTCAACTCAGAAGCACTCAA AAATGCAAAATGTGATAATGGGCACTTGTTTAAAAGAATTAGTGTATCCAGCCTTCACTCCAGCTGGTTAAAAATGTTGCACTTATCAGCAACCCTACCA CTTTCATCTGCTGAAAGGACAAATGTGCTTGGTTTTACTATTATGTAATCACAACTTACTTTCTGCTTGTAGTTGCTTAAAATTATGTATTTTGTCTTGG GCTGCAATTTGTTTTATGCTTATTTTATTATTACTGCAGTAGTTGACTTTGCTGTATGGAAAAATAAAGTGAAATTGCCCTAATAAAACTTCTCTTTCTT AAGTA

refseq_MBNL1.F3 (forward primer)
refseq_MBNL1.R3 (reverse primer)
Single exon skipping

Frame 0

LHLIAPSEL*YGAAVYLLWRQYGLLAVEYPGIS EVTYYF*RGSFAFSKSHGAKLGIQWPVNYKYYLQ TRRKADMCLCLCDSFTKEFQNCG*PVT*EKYTV AVLQMFFPPVSPPGSKTVWCILRRQSHQIAGF* SNWLLTFNLTLPASLQSPVQGPLSK*THWCA*QS VEEAGGRPVCTPAARLQC*THEMEDTNGRPRGS TAPARAPASSCSRRRWATAHDPLLRALSG*QAT LWPGEAREGAGGKLKSVFLAAACIRSSTSPPWAS WWGWAGGGGAAQQQRKPDLGDKRLHSDMQQSFH CS*MSCGAHNAPMTRS*QVPFSVAGILES*LPQ CLGHLVDSGAASEKKM*EVGTKKCLS*RLCCLEP LLEQSLLLPCLLPARL*RQHIHPPPLAQTPPFL LIIKRKALSIWHCPRLL*C*KKSLLKK*DIFCQ EIKEEKKNHFLDFALNCCICLCQTNQYRLHHQTP LQIQL*GDSLSDNFAESSLEIRCRRVCHVFMLA FWAPNWHWEGVTESTRLIPGPTFKRSSTYNPSI SLKTKTSLN*QFHAVNF*WEIFSLILTTQFSMYF *SREWGKVF*GDIFIISSAFFFWLLLFFGGVGF VGFTETFNYL*NLNMAVSVTPIRDTKWLTLEVC REFQRGTCSRPDTECKFAHPSKSCQVENGRVIAC FDSLKGRCSRENCKYLHPPPHLKTQLEINGRNN LIQQKNMAMLAQQMQLANAMMPGAPLQPVPMFS VAPSLATNASAAAFNPYLGPVSPSLVPAEILPTA PMLVTGNPGVPVPAAAAAAAQKLMRTDRLEVCR EYQRGNCNRGENDCRFAHPADSTMIDTNDNTVT VCMDYIKGRCSREKCKYFHPPAHLQAKIKAAQYQ VNQAAAAQAAATAAAMGIPQAVLPPLPKRPALE KTNGATAVFNTGIFQYQQALANMQLQQHTAFLP PGSILCMTPATSVVPMVHGATPATVSAATTSATS VPFAATATANQIPIISAEHLTSHKYVTQM*NFH H*TIMLKRKGQCAWLE*RTRSLAILYISSSNTH KSSSATRHPHIAC*PEEKTTFSGNPLHTVAYTLC TFN*YLC*GDIPV*KNNIVFLF*HRVMHSKMHT *LVSYIFMPS*KDRLWCNHDSIMYWYVCRPRYN FLKISLFLSRFTNNKGAPCI*NCHYR*ERACTVI FV*E*YF*CNRL*DVVREGSDRDECAKQNHNCV YFKAHHGFKYHVVKDSHEVP*TVHQIRVLFLQC YCFQSHILLHIC*Y*SVKGHHSFFFFLKPKLSQK WPI*LYSNNRQHNTNSLNTDKLTHTGDIYIIDI YKIILMHCSVIFMHTILYNMLFKRDCHF*DTVT KK*GNYFASIYSLCQKSKDYKCFAKMVSRLLKCF ITVTFKIVTLNKEESSTVIRCMINQNMKMGNV* LT**LGGLSTWVNFICDFCFVLFCSD*LLIALE SLLQN*KKNRCAFSF*EWNHPKEFLFLRFGCCS* *RIFLLCSAVLKIAEVGARSLVVIVWNGRSESS VIELSILPSLLQVFMLERDAF*YKTDVLILLIV LYIY*SLRLLHYGLEPIPM*FQSC*ESNGDFTCY CS*LHYRILLIFLVKM*FFISYIY*IFIFY*QL NTISVYRLVLLDFTNEFFKIQKKVVFPS*HTQF *ITCSVT*IFVLLTK*FIFY*FNITV*ECQSLLV LV*FSLKEQRSFIWISYKYIIIAK*EVKLLLSQ QSWQTIE*YFDDLFCL*LVIIRRSRS*ILE*NL FSTVSISNVKILFNIFEIPEYQALL*ISCIINK* NKGLFVDNPNTQSLRNENYSVCANS*GLIMLQF SMLERLGRRLTFCTSVYSQKRETCIIVRSYFE* KRL*LQGVLLRLIQ*QTEQNCLQKWHRVSTPYPF KHVALLSCGQLVVCQDFFSWKDTPSFQTLMTDK NSMGPNLPEDHYQK*QVLLPLR*NHGSDIPYIF QNYCMFKTSTKKERKNYTKNIYYSDQFLPISVVY CSQKNLQNKY*LSQNLNHKQANQTAHCSYSCYV IVF*LL*DPVLSAGEK*NAVQISWF*FSTQKHS KMQNVIMGTCLKELVYPAFTPAG*KCCTYQQPYH FHLLKGQMCLVLLLCNHNLLSACSCLKLCILSW AAICFMLILLLLQ*LTLLYGKIK*NCPNKTSLS *V

Frame 1

YIL*HPVNYDMGLRFICSGDNMAYLPWNIPVFQ K*HIIFKEGALLSLNHMVQSWEYSGL*ITNIIY KLEGKLTCVCACVTVSLRNSKTVDSLLLEKNTRW QSCRCFSRLLALQVQKLFGAF*GGSLTRSQDSS LIGF*PSI*PCRLPCKARCKGLFQNEPTGVPSS R*KKREGVRSARPPRGYNAERMRWKIPTGGRGDP RRPRGLRLPPALGAAGRPPMTRSCGLCPVDRRP CGPGKRGRAPAES*RAFFSPPRALGARRVRPGL PGGAGRAGEGPRSSSGSQTSAIRGCTATCNSLFT AAE*VVAPTMLP*QGADKFHFPSRASWNHDSHN ALGTWSTVGPPLKKKCERLVLRSAFPDVSAAWN RF*SSLCFCLACCQLDCDDSTSTLHL*PRHPHFY L*SREKL*VSGIALGCFSVKRKVC*KSKISSAR KSRRKKKIIFSILL*TAASVYAKLINTDCTTKL HCKFSCEEIPFQTTLLKAAWKFGVEGSATFSCLH FGLQIGTGKGLLRAQG*YQALLLNVHLLTILVF L*KPKPL*INSFML*ISSGRSFP*Y*RHNFPCT FKAGSGEKYFEGTFSSSVQLFFFGCCSFLGGLGL LVSLKHLTTCKI*TWLLVSHQFGTQNG*HWKYV ESSRGGLAHGQTRNVNLHILRKAAKLKMDE*SP ALIH*KAVAPGRTANIFIHPHI*KRSWR*MDAIT *FSRRTWPCWPSKCN*PMP*CLVPHYNPCQCFQ LHQA*PPMHQQPPLIPIWDLFLQAWSRQRSCRL HQCWLQGIRVSLYLQLLQLLHRN*CEQTDLRYVE STNVAIATEEKMIVGLLILLTAQ*LTPMTTQSL CVWITSKGDALGKSANTFIPLHICKPRSRLPNT RSTRLQLHRLQPPQLPWEFLKLYFPHYQRGLLLK KPTVPPQSLTLVFSNTNRL*PTCSYNSIQHFSH QAQYCA*HPLQVLFPWCTVLRQPLCPQQQHLPQ VFPSLQQPQPTRYP*YLPNI*LATSMLPRCRIFI TKQSC*RGKDSVLG*SKGRGH*PYCIYRQATHT KVPQPQDIHILHVNQKKRQHFPEIHCTLLPIHF VHLIDICAEVIFLSKRTTLSFFSSTELCIQRCIP S*FPIYSCHLEKTDYGVTMILLCIGTSVDQDII F*K*VYFFQGLQITKVHLVFKIAIIDESVHAQS FLFKSNIFNVIDCKTW*GRDLTEMNVPSKTTTVY ILKHIMALSTMLLRILMKCHRLYIKLEYYFFSV IVFRATFCCIFASTNQSKGTILFFFF*NQSCLR NGQFNFTVTIDSTTQTLSIQINSHILEIYI**IY IKLF*CIVV*YLCILYCITCYSKGIAISETQ*Q KNEEIILLLFIASVKSQKTINALQKWFHVCLNA SSQSHSK**L*TKKKAALSSDA**TKI*KWEMFN *PSNWVG*VHG*ILYVIFVLFCFVQINCL*P*K AFYKIKKKIDVHSVFKNGIIQRNSFF*GLDVAA SKGYFCSVQQF*KLLK*GPGHW*L*YGMGEVKVQ L*NFPYFQVYCKFLCLREMLSNIRLMC*FY*LY CTSIKALDYYITGWNPYQCNFNRVKKVMVTSHV IVVSYIIEYYLFFLLKCSFSFPTFIRFSFSINN* IPFQFIDLFY*ILPMNFSKYKKK*FFLHNILSF ELHVVSHEYSYC*LNDLYFTDLILQCKNVSHC* FLSSFH*KNKDLLYGYLINI*SLLSKKLSCCYRN NPGRQLSNILMIYFVCN*LL*EDLDPRY*NKIY FLLYPFQMLKYCLIFLKSLSIRPCYK*AA*SIN RTRDFLLIIQILKVYVMKIIACVQTLEG*LCCNL ACWNV*GEG*LFALLYIVKREKPV***DLILNK NVYNYKEFC*G*YNDRLSKIACKSGTELALHTP SNMLLCFLVDSL*FARIFSAGKIRHPFKPS*LTK TPWGQICLKIITKNSRYFYH*GEIMDQIFLTFF KTTACLKLQQKKRERTILRTYIIQISFCQFQWF IVHKKIFKTSIDFHKI*IINRQTKQHTVAIVVM* LFFNCCRILFFQQVKNKTQFKFHGFNFQLRSTQ KCKM**WALV*KN*CIQPSLQLVKNVALISNPT TFIC*KDKCAWFYYYVITTYFLLVVA*NYVFCLG LQFVLCLFYYYCSS*LCCMEK*SEIALIKLLFL K

Frame 2

TSYSTQ*IMIWGCGLFALATIWLTCRGISRYFR SNILFLKRELCFL*ITWCKVGNTVACKLQILFT N*KES*HVFVPV*QFH*GIPKLWIACYLRKIHG GSLADVFPAC*PSRFKNCLVHFKAAVSPDRRILV *LASNLQSNLAGFLAKPGARASFKMNPLVCLAV GRRSGRASGLHARREVTMLNA*DGRYQREAEGI HGARAGSGFLLLSAPLGDRP*PALAGSVRLTGDP VARGSAGGRRRKVEERFSRRRVH*ELDESALGF LVGLGGRGRGRAAAAEARPRR*EAAQRHATVFS LQLNELWRPQCSHDKELTSSIFRRGHLGIMTPTM PWALGRQWGRL*KKNVRGWY*EVPFLTSLLLGT ASRAVSAFALLAAS*TVTTAHPPSTSSPDTPIS TYNQEKSSKYLALP*AALVLKEKFAEKVRYLLPG NQGGKKKSFSRFCSKLLHLSMPN*SIPIAPPNS IANSAVRRFPFRQLC*KQLGNSVSKGLPRFHAC ILGSKLALGRGY*EHKADTRPYF*TFIYLQS*YF SKNQNLFELTVSCCEFLVGDLFLDIDDTIFHVL LKQGVGKSILRGHFHHQFSFFFLVVALFWGGWV CWFH*NI*LPVKSKHGC*CHTNSGHKMANTGSM* RVPEGDLLTARHGM*ICTSFEKLPS*KWTSNRL L*FIERPLLQGELQISSSTPTFKNAVGDKWTQ* LDSAEEHGHVGPANATSQCHDAWCPITTRANVFS CTKLSHQCISSRL*SLSGTCFSKPGPGRDLADC TNVGYRESGCPCTCSCCSCCTEINANRQT*GMS RVPTWQLQPRRK*LSVCSSC*QHND*HQ*QHSHC VYGLHQREMLSGKVQILSSPCTFASQDQGCPIP GQPGCSCTGCSHRSCHGNSSSCTSPITKEACS* KNQRCHRSL*HWYFPIPTGSSQHAVTTAYSISPT RLNIVHDTRYKCCSHGARCYASHCVRSNNICHK CSLRCNSHSQPDTHNICRTSD*PQVCYPDVEFS SLNNHAKEERTVCLVRVKDEVISHIVYIVKQHTQ KFLSHKTSTYCMLTRRKDNIFRKSTAHCCLYTL YI*LIFVLR*YSCLKEQHCLSFLAQSYAFKDAY LVSFLYIHAILKRQTMV*P*FYYVLVRL*TKI*F FKNKFISFKVYK*QRCTLYLKLPL*MRACMHSH FCLRVIFLM**IVRRGEGGI*QR*MCQAKPQLC IF*STSWL*VPCC*GFS*SAIDCTSN*SIISSVL LFSEPHFVAYLLVLISQRAPFFFFFFETKAVSE MANLTLQ*Q*TAQHKLSQYR*THTYWRYIYNRY I*NYFNAL*CNIYAYYTV*HVIQKGLPFLRHSNK KMRKLFCFYL*PLSKVKRL*MLCKNGFTFA*ML HHSHIQNSDSKQRRKQHCHQMHDKPKYENGKCL INLVIGWVKYMGEFYM*FLFCFVLFRLTAYSLRK PFTKLKKK*MCIQFLRMESSKGIPFFEVWMLQL VKDIFALFSSSKNC*SRGQVTGSYSMEWEK*KF SYRTFHTSKFTASFYA*ERCFLI*D*CVDFTDCT VHLLKP*IITLRVGTHTNVISIVLRK*W*LHML L*LVTL*NITYFSC*NVVFHFLHLLDFHFLLTI EYHFSL*TCFIRFYQ*IFQNTKKSSFSFITYSVL NYM*CHMNIRIVN*MIYILLI*YYSVRMSVIVS SCLVFIKRTKIFYMDIL*IYNHC*VRS*VVAIA TILADN*VIF**FILFVISYYKKI*ILDIRIKFI FYCIHFKC*NIV*YF*NP*VSGLVINKLHNQ*I EQGTFC**SKYSKFT**KL*RVCKLLRVDYAAI *HVGTSREKVDFLHFCI*SKERNLYNSKILF*IK TSIITRSFVKANTMTD*AKLLAKVAQS*HSIPL QTCCFAFLWTACSLPGFFQLERYAILSNPHD*Q KLHGAKSA*RSLPKIAGTSTIKVKSWIRYSLHFS KLLHV*NFNKKREKELY*EHILFRSVSANFSGL LFTKKSSKQVLTFTKFKS*TGKPNSTL*L*LLC DCFLIAVGSCSFSR*KIKRSSNFMVLIFNSEALK NAKCDNGHLFKRISVSSLHSSWLKMLHLSATLP LSSAERTNVLGFTIM*SQLTFCL*LLKIMYFVL GCNLFYAYFIITAVVDFAVWKNKVKLP**NFSFL S

Sequence NM_207293

GTTGGTACTAAGAAGTGCCTTTCCTGACGTCTCTGCTGCTTGGAACCGCTTCTAGAGCAGTCTCTGCTTTTGCCTTGCTTGCTGCCAGCTAGACTGTGAC GACAGCACATCCACCCTCCACCTCTAGCCCAGACACCCCCATTTCTACTTATAATCAAGAGAAAAGCTCTAAGTATCTGGCATTGCCCTAGGCTGCTTTA GTGTTAAAAGAAAAGTTTGCTGAAAAAGTAAGATATCTTCTGCCAGGAAATCAAGGAGGAAAAAAAAAATCATTTTCTCGATTTTGCTCTAAACTGCTGC ATCTGTCTATGCCAAACTAATCAATACCGATTGCACCACCAAACTCCATTGCAAATTCAGCTGTGAGGAGATTCCCTTTCAGACAACTTTGCTGAAAGCA GCTTGGAAATTCGGTGTCGAAGGGTCTGCCACGTTTTCATGCTTGCATTTTGGGCTCCAAATTGGCACTGGGAAGGGGTTACTGAGAGCACAAGGCTGAT ACCAGGCCCTACTTTTAAACGTTCATCTACTTACAATCCTAGTATTTCTCTAAAAACCAAAACCTCTTTGAATTAACAGTTTCATGCTGTGAATTTCTAG TGGGAGATCTTTTCCTTGATATTGACGACACAATTTTCCATGTACTTTTAAAGCAGGGAGTGGGGAAAAGTATTTTGAGGGGACATTTTCATCATCAGTT CAGCTTTTTTTTTTTGGTTGTTGCTCTTTTTTGGGGGGGTTGGGTTTGTTGGTTTCACTGAAACATTTAACTACCTGTAAAATCTAAACATGGCTGTTAG TGTCACACCAATTCGGGACACAAAATGGCTAACACTGGAAGTATGTAGAGAGTTCCAGAGGGGGACTTGCTCACGGCCAGACACGGAATGTAAATTTGCA CATCCTTCGAAAAGCTGCCAAGTTGAAAATGGACGAGTAATCGCCTGCTTTGATTCATTGAAAGGCCGTTGCTCCAGGGAGAACTGCAAATATCTTCATC CACCCCCACATTTAAAAACGCAGTTGGAGATAAATGGACGCAATAACTTGATTCAGCAGAAGAACATGGCCATGTTGGCCCAGCAAATGCAACTAGCCAA TGCCATGATGCCTGGTGCCCCATTACAACCCGTGCCAATGTTTTCAGTTGCACCAAGCTTAGCCACCAATGCATCAGCAGCCGCCTTTAATCCCTATCTG GGACCTGTTTCTCCAAGCCTGGTCCCGGCAGAGATCTTGCCGACTGCACCAATGTTGGTTACAGGGAATCCGGGTGTCCCTGTACCTGCAGCTGCTGCAG CTGCTGCACAGAAATTAATGCGAACAGACAGACTTGAGGTATGTCGAGAGTACCAACGTGGCAATTGCAACCGAGGAGAAAATGATTGTCGGTTTGCTCA TCCTGCTGACAGCACAATGATTGACACCAATGACAACACAGTCACTGTGTGTATGGATTACATCAAAGGGAGATGCTCTCGGGAAAAGTGCAAATACTTT CATCCCCCTGCACATTTGCAAGCCAAGATCAAGGCTGCCCAATACCAGGTCAACCAGGCTGCAGCTGCACAGGCTGCAGCCACCGCAGCTGCCATGACTC AGTCGGCTGTCAAATCACTGAAGCGACCCCTCGAGGCAACCTTTGACCTGGGAATTCCTCAAGCTGTACTTCCCCCATTACCAAAGAGGCCTGCTCTTGA AAAAACCAACGGTGCCACCGCAGTCTTTAACACTGGTATTTTCCAATACCAACAGGCTCTAGCCAACATGCAGTTACAACAGCATACAGCATTTCTCCCA CCAGTTCCCATGGTGCACGGTGCTACGCCAGCCACTGTGTCCGCAGCAACAACATCTGCCACAAGTGTTCCCTTCGCTGCAACAGCCACAGCCAACCAGA TACCCATAATATCTGCCGAACATCTGACTAGCCACAAGTATGTTACCCAGATGTAGAATTTTCATCACTAAACAATCATGCTAAAGAGGAAAGGACAGTG TGCTTGGTTAGAGTAAAGGACGAGGTCATTAGCCATATTGTATATATCGTCAAGCAACACACACAAAAGTTCCTCAGCCACAAGACATCCACATATTGCA TGTTAACCAGAAGAAAAGACAACATTTTCCGGAAATCCACTGCACACTGTTGCCTATACACTTTGTACATTTAATTGATATTTGTGCTGAGGTGATATTC CTGTCTAAAAGAACAACATTGTCTTTCTTTTCTAGCACAGAGTTATGCATTCAAAGATGCATACCTAGTTAGTTTCCTATATATTCATGCCATCTTGAAA AGACAGACTATGGTGTAACCATGATTCTATTATGTATTGGTACGTCTGTAGACCAAGATATAATTTTTTAAAAATAAGTTTATTTCTTTCAAGGTTTACA AATAACAAAGGTGCACCTTGTATTTAAAATTGCCATTATAGATGAGAGCGTGCATGCACAGTCATTTTTGTTTAAGAGTAATATTTTTAATGTAATAGAT TGTAAGACGTGGTGAGGGAGGGATCTGACAGAGATGAATGTGCCAAGCAAAACCACAACTGTGTATATTTTAAAGCACATCATGGCTTTAAGTACCATGT TGTTAAGGATTCTCATGAAGTGCCATAGACTGTACATCAAATTAGAGTATTATTTCTTCAGTGTTATTGTTTTCAGAGCCACATTTTGTTGCATATTTGC TAGTACTAATCAGTCAAAGGGCACCATTCTTTTTTTTTTTTTTTGAAACCAAAGCTGTCTCAGAAATGGCCAATTTAACTTTACAGTAACAATAGACAGC ACAACACAAACTCTCTCAATACAGATAAACTCACACATACTGGAGATATATATATAATAGATATATATAAAATTATTTTAATGCATTGTAGTGTAATATT TATGCATACTATACTGTATAACATGTTATTCAAAAGGGATTGCCATTTCTGAGACACAGTAACAAAAAAATGAGGAAATTATTTTGCTTCTATTTATAGC CTCTGTCAAAAGTCAAAAGACTATAAATGCTTTGCAAAAATGGTTTCACGTTTGCTTAAATGCTTCATCACAGTCACATTCAAAATAGTGACTCTAAACA AAGAAGAAAGCAGCACTGTCATCAGATGCATGATAAACCAAAATATGAAAATGGGAAATGTTTAATTAACCTAGTAATTGGGTGGGTTAAGTACATGGGT GAATTTTATATGTGATTTTTGTTTTGTTTTGTTTTGTTCAGATTAACTGCTTATAGCCTTAGAAAGCCTTTTACAAAATTAAAAAAAAAATAGATGTGCA TTCAGTTTTTAAGAATGGAATCATCCAAAGGAATTCCTTTTTTTGAGGTTTGGATGTTGCAGCTAGTAAAGGATATTTTTGCTCTGTTCAGCAGTTCTAA AAATTGCTGAAGTAGGGGCCAGGTCACTGGTAGTTATAGTATGGAATGGGAGAAGTGAAAGTTCAGTTATAGAACTTTCCATACTTCCAAGTTTACTGCA AGTTTTTATGCTTGAGAGAGATGCTTTCTAATATAAGACTGATGTGTTGATTTTACTGATTGTACTGTACATCTATTAAAGCCTTAGATTATTACATTAC GGGTTGGAACCCATACCAATGTAATTTCAATCGTGTTAAGAAAGTAATGGTGACTTCACATGTTATTGTAGTTAGTTACATTATAGAATATTACTTATTT TTCTTGTTAAAATGTAGTTTTTCATTTCCTACATTTATTAGATTTTCATTTTCTATTAACAATTGAATACCATTTCAGTTTATAGACTTGTTTTATTAGA TTTTACCAATGAATTTTTCAAAATACAAAAAAAAGTAGTTTTTCCTTCATAACATACTCAGTTTTGAATTACATGTAGTGTCACATGAATATTCGTATTG TTAACTAAATGATTTATATTTTACTGATTTAATATTACAGTGTAAGAATGTCAGTCATTGTTAGTTCTTGTCTAGTTTTCATTAAAAGAACAAAGATCTT TTATATGGATATCTTATAAATATATAATCATTGCTAAGTAAGAAGTTAAGTTGTTGCTATCGCAACAATCCTGGCAGACAATTGAGTAATATTTTGATGA TTTATTTTGTTTGTAATTAGTTATTATAAGAAGATCTAGATCCTAGATATTAGAATAAAATTTATTTTCTACTGTATCCATTTCAAATGTTAAAATATTG TTTAATATTTTTGAAATCCCTGAGTATCAGGCCTTGTTATAAATAAGCTGCATAATCAATAAATAGAACAAGGGACTTTTTGTTGATAATCCAAATACTC AAAGTTTACGTAATGAAAATTATAGCGTGTGTGCAAACTCTTGAGGGTTGATTATGCTGCAATTTAGCATGTTGGAACGTCTAGGGAGAAGGTTGACTTT TTGCACTTCTGTATATAGTCAAAAGAGAGAAACCTGTATAATAGTAAGATCTTATTTTGAATAAAAACGTCTATAATTACAAGGAGTTTTGTTAAGGCTA ATACAATGACAGACTGAGCAAAATTGCTTGCAAAAGTGGCACAGAGTTAGCACTCCATACCCCTTCAAACATGTTGCTTTGCTTTCTTGTGGACAGCTTG TAGTTTGCCAGGATTTTTTCAGCTGGAAAGATACGCCATCCTTTCAAACCCTCATGACTGACAAAAACTCCATGGGGCCAAATCTGCCTGAAGATCATTA CCAAAAATAGCAGGTACTTCTACCATTAAGGTGAAATCATGGATCAGATATTCCTTACATTTTTCAAAACTACTGCATGTTTAAAACTTCAACAAAAAAA GAGAGAAAGAACTATACTAAGAACATATATTATTCAGATCAGTTTCTGCCAATTTCAGTGGTTTATTGTTCACAAAAAAATCTTCAAAACAAGTATTGAC TTTCACAAAATTTAAATCATAAACAGGCAAACCAAACAGCACACTGTAGCTATAGTTGTTATGTGATTGTTTTTTAATTGCTGTAGGATCCTGTTCTTTC AGCAGGTGAAAAATAAAACGCAGTTCAAATTTCATGGTTTTAATTTTCAACTCAGAAGCACTCAAAAATGCAAAATGTGATAATGGGCACTTGTTTAAAA GAATTAGTGTATCCAGCCTTCACTCCAGCTGGTTAAAAATGTTGCACTTATCAGCAACCCTACCACTTTCATCTGCTGAAAGGACAAATGTGCTTGGTTT TACTATTATGTAATCACAACTTACTTTCTGCTTGTAGTTGCTTAAAATTATGTATTTTGTCTTGGGCTGCAATTTGTTTTATGCTTATTTTATTATTACT GCAGTAGTTGACTTTGCTGTATGGAAAAATAAAGTGAAATTGCCCTAATAAAACTTCTCTTTCTTAAGTA

refseq_MBNL1.F3 (forward primer)
refseq_MBNL1.R3 (reverse primer)
Single exon skipping

Frame 0

VGTKKCLS*RLCCLEPLLEQSLLLPCLLPARL* RQHIHPPPLAQTPPFLLIIKRKALSIWHCPRLL* C*KKSLLKK*DIFCQEIKEEKKNHFLDFALNCC ICLCQTNQYRLHHQTPLQIQL*GDSLSDNFAES SLEIRCRRVCHVFMLAFWAPNWHWEGVTESTRLI PGPTFKRSSTYNPSISLKTKTSLN*QFHAVNF* WEIFSLILTTQFSMYF*SREWGKVF*GDIFIIS SAFFFWLLLFFGGVGFVGFTETFNYL*NLNMAVS VTPIRDTKWLTLEVCREFQRGTCSRPDTECKFA HPSKSCQVENGRVIACFDSLKGRCSRENCKYLH PPPHLKTQLEINGRNNLIQQKNMAMLAQQMQLAN AMMPGAPLQPVPMFSVAPSLATNASAAAFNPYL GPVSPSLVPAEILPTAPMLVTGNPGVPVPAAAA AAAQKLMRTDRLEVCREYQRGNCNRGENDCRFAH PADSTMIDTNDNTVTVCMDYIKGRCSREKCKYF HPPAHLQAKIKAAQYQVNQAAAAQAAATAAAMT QSAVKSLKRPLEATFDLGIPQAVLPPLPKRPALE KTNGATAVFNTGIFQYQQALANMQLQQHTAFLP PVPMVHGATPATVSAATTSATSVPFAATATANQ IPIISAEHLTSHKYVTQM*NFHH*TIMLKRKGQC AWLE*RTRSLAILYISSSNTHKSSSATRHPHIA C*PEEKTTFSGNPLHTVAYTLCTFN*YLC*GDI PV*KNNIVFLF*HRVMHSKMHT*LVSYIFMPS*K DRLWCNHDSIMYWYVCRPRYNFLKISLFLSRFT NNKGAPCI*NCHYR*ERACTVIFV*E*YF*CNR L*DVVREGSDRDECAKQNHNCVYFKAHHGFKYHV VKDSHEVP*TVHQIRVLFLQCYCFQSHILLHIC *Y*SVKGHHSFFFFLKPKLSQKWPI*LYSNNRQ HNTNSLNTDKLTHTGDIYIIDIYKIILMHCSVIF MHTILYNMLFKRDCHF*DTVTKK*GNYFASIYS LCQKSKDYKCFAKMVSRLLKCFITVTFKIVTLN KEESSTVIRCMINQNMKMGNV*LT**LGGLSTWV NFICDFCFVLFCSD*LLIALESLLQN*KKNRCA FSF*EWNHPKEFLFLRFGCCS**RIFLLCSAVL KIAEVGARSLVVIVWNGRSESSVIELSILPSLLQ VFMLERDAF*YKTDVLILLIVLYIY*SLRLLHY GLEPIPM*FQSC*ESNGDFTCYCS*LHYRILLI FLVKM*FFISYIY*IFIFY*QLNTISVYRLVLLD FTNEFFKIQKKVVFPS*HTQF*ITCSVT*IFVL LTK*FIFY*FNITV*ECQSLLVLV*FSLKEQRS FIWISYKYIIIAK*EVKLLLSQQSWQTIE*YFDD LFCL*LVIIRRSRS*ILE*NLFSTVSISNVKIL FNIFEIPEYQALL*ISCIINK*NKGLFVDNPNT QSLRNENYSVCANS*GLIMLQFSMLERLGRRLTF CTSVYSQKRETCIIVRSYFE*KRL*LQGVLLRL IQ*QTEQNCLQKWHRVSTPYPFKHVALLSCGQL VVCQDFFSWKDTPSFQTLMTDKNSMGPNLPEDHY QK*QVLLPLR*NHGSDIPYIFQNYCMFKTSTKK ERKNYTKNIYYSDQFLPISVVYCSQKNLQNKY* LSQNLNHKQANQTAHCSYSCYVIVF*LL*DPVLS AGEK*NAVQISWF*FSTQKHSKMQNVIMGTCLK ELVYPAFTPAG*KCCTYQQPYHFHLLKGQMCLV LLLCNHNLLSACSCLKLCILSWAAICFMLILLLL Q*LTLLYGKIK*NCPNKTSLS*V

Frame 1

LVLRSAFPDVSAAWNRF*SSLCFCLACCQLDCD DSTSTLHL*PRHPHFYL*SREKL*VSGIALGCF SVKRKVC*KSKISSARKSRRKKKIIFSILL*TAA SVYAKLINTDCTTKLHCKFSCEEIPFQTTLLKA AWKFGVEGSATFSCLHFGLQIGTGKGLLRAQG* YQALLLNVHLLTILVFL*KPKPL*INSFML*ISS GRSFP*Y*RHNFPCTFKAGSGEKYFEGTFSSSV QLFFFGCCSFLGGLGLLVSLKHLTTCKI*TWLL VSHQFGTQNG*HWKYVESSRGGLAHGQTRNVNLH ILRKAAKLKMDE*SPALIH*KAVAPGRTANIFI HPHI*KRSWR*MDAIT*FSRRTWPCWPSKCN*P MP*CLVPHYNPCQCFQLHQA*PPMHQQPPLIPIW DLFLQAWSRQRSCRLHQCWLQGIRVSLYLQLLQ LLHRN*CEQTDLRYVESTNVAIATEEKMIVGLL ILLTAQ*LTPMTTQSLCVWITSKGDALGKSANTF IPLHICKPRSRLPNTRSTRLQLHRLQPPQLP*L SRLSNH*SDPSRQPLTWEFLKLYFPHYQRGLLL KKPTVPPQSLTLVFSNTNRL*PTCSYNSIQHFSH QFPWCTVLRQPLCPQQQHLPQVFPSLQQPQPTR YP*YLPNI*LATSMLPRCRIFITKQSC*RGKDS VLG*SKGRGH*PYCIYRQATHTKVPQPQDIHILH VNQKKRQHFPEIHCTLLPIHFVHLIDICAEVIF LSKRTTLSFFSSTELCIQRCIPS*FPIYSCHLE KTDYGVTMILLCIGTSVDQDIIF*K*VYFFQGLQ ITKVHLVFKIAIIDESVHAQSFLFKSNIFNVID CKTW*GRDLTEMNVPSKTTTVYILKHIMALSTM LLRILMKCHRLYIKLEYYFFSVIVFRATFCCIFA STNQSKGTILFFFF*NQSCLRNGQFNFTVTIDS TTQTLSIQINSHILEIYI**IYIKLF*CIVV*Y LCILYCITCYSKGIAISETQ*QKNEEIILLLFIA SVKSQKTINALQKWFHVCLNASSQSHSK**L*T KKKAALSSDA**TKI*KWEMFN*PSNWVG*VHG *ILYVIFVLFCFVQINCL*P*KAFYKIKKKIDVH SVFKNGIIQRNSFF*GLDVAASKGYFCSVQQF* KLLK*GPGHW*L*YGMGEVKVQL*NFPYFQVYC KFLCLREMLSNIRLMC*FY*LYCTSIKALDYYIT GWNPYQCNFNRVKKVMVTSHVIVVSYIIEYYLF FLLKCSFSFPTFIRFSFSINN*IPFQFIDLFY* ILPMNFSKYKKK*FFLHNILSFELHVVSHEYSYC *LNDLYFTDLILQCKNVSHC*FLSSFH*KNKDL LYGYLINI*SLLSKKLSCCYRNNPGRQLSNILM IYFVCN*LL*EDLDPRY*NKIYFLLYPFQMLKYC LIFLKSLSIRPCYK*AA*SINRTRDFLLIIQIL KVYVMKIIACVQTLEG*LCCNLACWNV*GEG*L FALLYIVKREKPV***DLILNKNVYNYKEFC*G* YNDRLSKIACKSGTELALHTPSNMLLCFLVDSL *FARIFSAGKIRHPFKPS*LTKTPWGQICLKII TKNSRYFYH*GEIMDQIFLTFFKTTACLKLQQKK RERTILRTYIIQISFCQFQWFIVHKKIFKTSID FHKI*IINRQTKQHTVAIVVM*LFFNCCRILFF QQVKNKTQFKFHGFNFQLRSTQKCKM**WALV*K N*CIQPSLQLVKNVALISNPTTFIC*KDKCAWF YYYVITTYFLLVVA*NYVFCLGLQFVLCLFYYY CSS*LCCMEK*SEIALIKLLFLK

Frame 2

WY*EVPFLTSLLLGTASRAVSAFALLAAS*TVT TAHPPSTSSPDTPISTYNQEKSSKYLALP*AAL VLKEKFAEKVRYLLPGNQGGKKKSFSRFCSKLL HLSMPN*SIPIAPPNSIANSAVRRFPFRQLC*KQ LGNSVSKGLPRFHACILGSKLALGRGY*EHKAD TRPYF*TFIYLQS*YFSKNQNLFELTVSCCEFL VGDLFLDIDDTIFHVLLKQGVGKSILRGHFHHQF SFFFLVVALFWGGWVCWFH*NI*LPVKSKHGC* CHTNSGHKMANTGSM*RVPEGDLLTARHGM*IC TSFEKLPS*KWTSNRLL*FIERPLLQGELQISSS TPTFKNAVGDKWTQ*LDSAEEHGHVGPANATSQ CHDAWCPITTRANVFSCTKLSHQCISSRL*SLS GTCFSKPGPGRDLADCTNVGYRESGCPCTCSCCS CCTEINANRQT*GMSRVPTWQLQPRRK*LSVCS SC*QHND*HQ*QHSHCVYGLHQREMLSGKVQIL SSPCTFASQDQGCPIPGQPGCSCTGCSHRSCHDS VGCQITEATPRGNL*PGNSSSCTSPITKEACS* KNQRCHRSL*HWYFPIPTGSSQHAVTTAYSISP TSSHGARCYASHCVRSNNICHKCSLRCNSHSQPD THNICRTSD*PQVCYPDVEFSSLNNHAKEERTV CLVRVKDEVISHIVYIVKQHTQKFLSHKTSTYC MLTRRKDNIFRKSTAHCCLYTLYI*LIFVLR*YS CLKEQHCLSFLAQSYAFKDAYLVSFLYIHAILK RQTMV*P*FYYVLVRL*TKI*FFKNKFISFKVY K*QRCTLYLKLPL*MRACMHSHFCLRVIFLM**I VRRGEGGI*QR*MCQAKPQLCIF*STSWL*VPC C*GFS*SAIDCTSN*SIISSVLLFSEPHFVAYL LVLISQRAPFFFFFFETKAVSEMANLTLQ*Q*TA QHKLSQYR*THTYWRYIYNRYI*NYFNAL*CNI YAYYTV*HVIQKGLPFLRHSNKKMRKLFCFYL* PLSKVKRL*MLCKNGFTFA*MLHHSHIQNSDSKQ RRKQHCHQMHDKPKYENGKCLINLVIGWVKYMG EFYM*FLFCFVLFRLTAYSLRKPFTKLKKK*MC IQFLRMESSKGIPFFEVWMLQLVKDIFALFSSSK NC*SRGQVTGSYSMEWEK*KFSYRTFHTSKFTA SFYA*ERCFLI*D*CVDFTDCTVHLLKP*IITL RVGTHTNVISIVLRK*W*LHMLL*LVTL*NITYF SC*NVVFHFLHLLDFHFLLTIEYHFSL*TCFIR FYQ*IFQNTKKSSFSFITYSVLNYM*CHMNIRI VN*MIYILLI*YYSVRMSVIVSSCLVFIKRTKIF YMDIL*IYNHC*VRS*VVAIATILADN*VIF** FILFVISYYKKI*ILDIRIKFIFYCIHFKC*NI V*YF*NP*VSGLVINKLHNQ*IEQGTFC**SKYS KFT**KL*RVCKLLRVDYAAI*HVGTSREKVDF LHFCI*SKERNLYNSKILF*IKTSIITRSFVKA NTMTD*AKLLAKVAQS*HSIPLQTCCFAFLWTAC SLPGFFQLERYAILSNPHD*QKLHGAKSA*RSL PKIAGTSTIKVKSWIRYSLHFSKLLHV*NFNKK REKELY*EHILFRSVSANFSGLLFTKKSSKQVLT FTKFKS*TGKPNSTL*L*LLCDCFLIAVGSCSF SR*KIKRSSNFMVLIFNSEALKNAKCDNGHLFK RISVSSLHSSWLKMLHLSATLPLSSAERTNVLGF TIM*SQLTFCL*LLKIMYFVLGCNLFYAYFIIT AVVDFAVWKNKVKLP**NFSFLS