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Sequences

Sequence NM_206946

AGCTGCAGCAAAATGAAAGCAACAGGAGCTGCTCCGGGGACTGCTTTTGCCAGTACCCAGAATCAGTGCTCAGGCTCAGAAATCCTGGATAGAAAGAGCA TTTTATAAAAGAGAATGTGTCCACATCATACCCAGCACCAAAGACCCCCATAGGTGTTGCTGTGGGCGTCTGATAGGCCAGCATGTTGGCCTCACCCCCA GTATCTCCGTGCTTCAGAATGAGAAAAATGAAAGTCGCCTCTCCCGAAATGACATCCAGTCTGAAAAGTGGTCCATCAGCAAACACACTCAACTCAGCCC TACGGATGCTTTTGGGACCATTGAGTTCCAAGGAGGTGGCCATTCCAACAAAGCCATGTATGTGCGAGTATCTTTTGATACAAAACCTGATCTCCTCTTA CACCTGATGACCAAGGAATGGCAGTTGGAGCTTCCCAAGCTTCTCATCTCTGTCCATGGGGGCCTGCAGAACTTTGAACTCCAGCCAAAACTCAAGCAAG TCTTTGGGAAAGGGCTCATCAAAGCAGCAATGACAACTGGAGCGTGGATATTCACTGGAGGGGTTAACACAGGTGTTATTCGTCATGTTGGCGATGCCTT GAAGGATCATGCCTCTAAGTCTCGAGGAAAGATATGCACCATAGGTATTGCCCCCTGGGGAATTGTGGAAAACCAGGAGGACCTCATTGGAAGAGATGTT GTCCGGCCATACCAGACCATGTCCAATCCCATGAGCAAGCTCACTGTTCTCAACAGCATGCATTCCCACTTCATTCTGGCTGACAACGGGACCACTGGAA AATATGGAGCAGAGGTGAAACTTCGAAGACAACTGGAAAAGCATATTTCACTCCAGAAGATAAACACAAGATGCCTGCCGTTTTTCTCTCTTGACTCCCG CTTGTTTTATTCATTTTGGGGTAGTTGCCAGTTAGACTCAGTTGGAATCGGTCAAGGTGTTCCTGTGGTGGCACTCATAGTGGAAGGAGGACCCAATGTG ATCTCGATTGTTTTGGAGTACCTTCGAGACACCCCTCCCGTGCCAGTGGTTGTCTGTGATGGGAGTGGACGGGCATCGGACATCCTGGCCTTTGGGCATA AATACTCAGAAGAAGGCGGACTGATAAATGAATCTTTGAGGGACCAGCTGTTGGTGACTATACAGAAGACTTTCACATACACTCGAACCCAAGCTCAGCA TCTGTTCATCATCCTCATGGAGTGCATGAAGAAGAAGGAATTGATTACGGTATTTCGGATGGGATCAGAAGGACACCAGGACATTGATTTGGCTATCCTG ACAGCTTTACTCAAAGGAGCCAATGCCTCGGCCCCAGACCAACTGAGCTTAGCTTTAGCCTGGAACAGAGTCGACATCGCTCGCAGCCAGATCTTTATTT ACGGGCAACAGTGGCCGGTGGGATCTCTGGAGCAAGCCATGTTGGATGCCTTAGTTCTGGACAGAGTGGATTTTGTGAAATTACTCATAGAGAATGGAGT AAGCATGCACCGTTTTCTCACCATCTCCAGACTAGAGGAATTGTACAATACGAGACATGGGCCCTCAAATACATTGTACCACTTGGTCAGGGATGTCAAA AAGGGGAACCTGCCCCCAGACTACAGAATCAGCCTGATTGACATCGGCCTGGTGATCGAGTACCTGATGGGCGGGGCTTATCGCTGCAACTACACGCGCA AGCGCTTCCGGACCCTCTACCACAACCTCTTCGGCCCCAAGAGGCCCAAAGCCTTGAAACTGCTGGGAATGGAGGATGATATTCCCTTGAGGCGAGGAAG AAAGACAACCAAGAAACGTGAAGAAGAGGTGGACATTGACTTGGATGATCCTGAGATCAACCACTTCCCCTTCCCTTTCCATGAGCTCATGGTGTGGGCT GTTCTCATGAAGCGGCAGAAGATGGCCCTGTTCTTCTGGCAGCACGGTGAGGAGGCCATGGCCAAGGCCCTGGTGGCCTGCAAGCTCTGCAAAGCCATGG CTCATGAGGCCTCTGAGAACGACATGGTTGACGACATTTCCCAGGAGCTGAATCACAATTCCAGAGACTTTGGCCAGCTGGCTGTGGAGCTCCTGGACCA GTCCTACAAGCAGGACGAACAGCTGGCCATGAAACTGCTGACGTATGAGCTGAAGAACTGGAGCAACGCCACGTGCCTGCAGCTTGCCGTGGCTGCCAAA CACCGCGACTTCATCGCGCACACGTGCAGCCAGATGCTGCTCACCGACATGTGGATGGGCCGGCTCCGCATGCGCAAGAACTCAGGCCTCAAGGTAATTC TGGGAATTCTACTTCCTCCTTCAATTCTCAGCTTGGAGTTCAAGAACAAAGACGACATGCCCTATATGTCTCAGGCCCAGGAAATCCACCTCCAAGAGAA GGAGGCAGAAGAACCAGAGAAGCCCACAAAGGAAAAAGAGGAAGAGGACATGGAGCTCACAGCAATGTTGGGACGAAACAACGGGGAGTCCTCCAGGAAG AAGGATGAAGAGGAAGTTCAGAGCAAGCACCGGTTAATCCCCCTCGGCAGAAAAATCTATGAATTCTACAATGCACCCATCGTGAAGTTCTGGTTCTACA CACTGGCGTATATCGGATACCTGATGCTCTTCAACTATATCGTGTTAGTGAAGATGGAACGCTGGCCGTCCACCCAGGAATGGATCGTAATCTCCTATAT TTTCACCCTGGGAATAGAAAAGATGAGAGAGATTCTGATGTCAGAGCCAGGGAAGTTGCTACAGAAAGTGAAGGTATGGCTGCAGGAGTACTGGAATGTC ACGGACCTCATCGCCATCCTTCTGTTTTCTGTCGGAATGATCCTTCGTCTCCAAGACCAGCCCTTCAGGAGTGACGGGAGGGTCATCTACTGCGTGAACA TCATTTACTGGTATATCCGTCTCCTAGACATCTTCGGCGTGAACAAGTATTTGGGCCCGTATGTAATGATGATTGGAAAAATGATGATAGACATGATGTA CTTTGTCATCATTATGCTGGTGGTTCTGATGAGCTTTGGGGTCGCCAGGCAAGCCATCCTTTTTCCCAATGAGGAGCCATCATGGAAACTGGCCAAGAAC ATCTTCTACATGCCCTATTGGATGATTTATGGGGAAGTGTTTGCGGACCAGATAGACCCTCCCTGTGGACAGAATGAGACCCGAGAGGATGGTAAAATAA TCCAGCTGCCTCCCTGCAAGACAGGAGCTTGGATCGTGCCGGCCATCATGGCCTGCTACCTCTTAGTGGCAAACATCTTGCTGGTCAACCTCCTCATTGC TGTCTTTAACAATACATTTTTTGAAGTAAAATCGATATCCAACCAAGTCTGGAAGTTTCAGAGGTATCAGCTCATCATGACTTTCCATGAAAGGCCAGTT CTGCCCCCACCACTGATCATCTTCAGCCACATGACCATGATATTCCAGCACCTGTGCTGCCGATGGAGGAAACACGAGAGCGACCCGGATGAAAGGGACT ACGGCCTGAAACTCTTCATAACCGATGATGAGCTCAAGAAAGTACATGACTTTGAAGAGCAATGCATAGAAGAATACTTCAGAGAAAAGGATGATCGGTT CAACTCATCTAATGATGAGAGGATACGGGTGACTTCAGAAAGGGTGGAGAACATGTCTATGCGGCTGGAGGAAGTCAACGAGAGAGAGCACTCCATGAAG GCTTCACTCCAGACCGTGGACATCCGGCTGGCGCAGCTGGAAGACCTTATCGGGCGCATGGCCACGGCCCTGGAGCGCCTGACAGGTCTGGAGCGGGCCG AGTCCAACAAAATCCGCTCGAGGACCTCGTCAGACTGCACGGACGCCGCCTACATTGTCCGTCAGAGCAGCTTCAACAGCCAGGAAGGGAACACCTTCAA GCTCCAAGAGAGTATAGACCCTGCAGGTGAGGAGACCATGTCCCCAACTTCTCCAACCTTAATGCCCCGTATGCGAAGCCATTCTTTCTATTCGGTCAAT ATGAAAGACAAAGGTGGTATAGAAAAGTTGGAAAGTATTTTTAAAGAAAGGTCCCTGAGCCTACACCGGGCTACTAGTTCCCACTCTGTAGCAAAAGAAC CCAAAGCTCCTGCAGCCCCTGCCAACACCTTGGCCATTGTTCCTGATTCCAGAAGACCATCATCGTGTATAGACATCTATGTCTCTGCTATGGATGAGCT CCACTGTGATATAGACCCTCTGGACAATTCCGTGAACATCCTTGGGCTGGGCGAGCCAAGCTTTTCAACTCCAGTACCTTCCACAGCCCCTTCAAGTAGT GCCTATGCAACACTTGCACCCACAGACAGACCTCCAAGCCGGAGCATTGATTTTGAGGACATCACCTCCATGGACACTAGATCTTTTTCTTCAGACTACA CCCACCTCCCAGAATGCCAAAACCCCTGGGACTCAGAGCCTCCGATGTACCACACCATTGAGCGTTCCAAAAGTAGCCGCTACCTAGCCACCACACCCTT TCTTCTAGAAGAGGCTCCCATTGTGAAATCTCATAGCTTTATGTTTTCCCCCTCAAGGAGCTATTATGCCAACTTTGGGGTGCCTGTAAAAACAGCAGAA TACACAAGTATTACAGACTGTATTGACACAAGGTGTGTCAATGCCCCTCAAGCAATTGCGGACAGAGCTGCCTTCCCTGGAGGTCTTGGAGACAAAGTGG AGGACTTAACTTGCTGCCATCCAGAGCGAGAAGCAGAACTGAGTCACCCCAGCTCTGACAGTGAGGAGAATGAGGCCAAAGGCCGCAGAGCCACCATTGC AATATCCTCCCAGGAGGGTGATAACTCAGAGAGAACCCTGTCCAACAACATCACTGTTCCCAAGATAGAGCGCGCCAACAGCTACTCGGCAGAGGAGCCA AGTGCGCCATATGCACACACCAGGAAGAGCTTCTCCATCAGTGACAAACTCGACAGGCAGCGGAACACAGCAAGCCTGCGAAATCCCTTCCAGAGAAGCA AGTCCTCCAAGCCGGAGGGCCGAGGGGACAGCCTGTCCATGAGGAGACTGTCCAGAACATCGGCTTTCCAAAGCTTTGAAAGCAAGCACAACTAAACCTT CTTAATATCCGCCACAGAAGGCTCAAGAATCCAGCCCTAAAATTCTCTCCAACTCCAGTTTTTCCCCTTTCCTTGAATCATACCTGCTTTATTCTTAGCT GAGCAAAACAAGCAATGCTTTGGGAGGTGTTAACTCAAAGGTGACTTCTGGGCCACAGATCAAGAAAGCATTTGATCTGACCCAGTGCCAGACACAGGGG ATTTAAGGCATGTTCACACTTGCTGGGCAGGGAGGGGGAAGAGAGGGAGAAGGAAGGGTTAGAGATGAATGTGTATCCGCAGTCACAGCAGAAAGCCATG AGAGCAGGGGAAACAAGGGGCTTCGAGCACGCTCCATGCCAGGAGGCATCTGTTGATTTCTGACCATTATCAAGAGTTGTAGGATGCAGGGCTAAATTGC AAAATAAAATAAAATAGCCAGCGTACACAATGAGATATTCTAAACTTCCATTCTGTTTTCTTTTCACATTGGCTCCATCACTGGTGACTGATGAAGAGCA TCCTCTTTATTCAGTATAAGCCGGCAGCAAGCAGTTCTACCTAACGTCCCACATCCTTCTCATGCCAACACTTCTGTAATTGATCATTATAAAGAAAAAA CAAGGTAACAGTCATAGTTCACCTGTCTCTTATCTATTCACTTCTGGTGCCACAACTGTTTATCCTTTTTTGAAGAAAATAAGGGAACAGAAATGCCTTT TTGTATTGCAATCGAAATGAAAGAAGAGTTGATGTTAAAAAAACAAAAGTCAAGTGATTTATTATATACAGTGGGCGTTCAAGTCTAGTCGAGCAAGCTC AGGAGAATGTAATTAAATAATTTTATATTTTTTAATTTATTTTGTATCTCACCTGTCATGGATGAATTCATTCACTGAATATGTAATATTGAACTTAAAA AAAAAAAAAAAAAAA

refseq_TRPM3.F2 (forward primer)
refseq_TRPM3.R2 (reverse primer)
Single exon skipping

Frame 0

SCSKMKATGAAPGTAFASTQNQCSGSEILDRKS IL*KRMCPHHTQHQRPP*VLLWASDRPACWPHPQ YLRASE*EK*KSPLPK*HPV*KVVHQQTHSTQP YGCFWDH*VPRRWPFQQSHVCASIF*YKT*SPL TPDDQGMAVGASQASHLCPWGPAEL*TPAKTQAS LWERAHQSSNDNWSVDIHWRG*HRCYSSCWRCL EGSCL*VSRKDMHHRYCPLGNCGKPGGPHWKRC CPAIPDHVQSHEQAHCSQQHAFPLHSG*QRDHWK IWSRGETSKTTGKAYFTPEDKHKMPAVFLS*LP LVLFILG*LPVRLSWNRSRCSCGGTHSGRRTQC DLDCFGVPSRHPSRASGCL*WEWTGIGHPGLWA* ILRRRRTDK*IFEGPAVGDYTEDFHIHSNPSSA SVHHPHGVHEEEGIDYGISDGIRRTPGH*FGYP DSFTQRSQCLGPRPTELSFSLEQSRHRSQPDLYL RATVAGGISGASHVGCLSSGQSGFCEITHREWS KHAPFSHHLQTRGIVQYETWALKYIVPLGQGCQ KGEPAPRLQNQPD*HRPGDRVPDGRGLSLQLHAQ ALPDPLPQPLRPQEAQSLETAGNGG*YSLEARK KDNQET*RRGGH*LG*S*DQPLPLPFP*AHGVG CSHEAAEDGPVLLAAR*GGHGQGPGGLQALQSHG S*GL*ERHG*RHFPGAESQFQRLWPAGCGAPGP VLQAGRTAGHETADV*AEELEQRHVPAACRGCQ TPRLHRAHVQPDAAHRHVDGPAPHAQELRPQGNS GNSTSSFNSQLGVQEQRRHALYVSGPGNPPPRE GGRRTREAHKGKRGRGHGAHSNVGTKQRGVLQE EG*RGSSEQAPVNPPRQKNL*ILQCTHREVLVLH TGVYRIPDALQLYRVSEDGTLAVHPGMDRNLLY FHPGNRKDERDSDVRAREVATESEGMAAGVLEC HGPHRHPSVFCRNDPSSPRPALQE*REGHLLREH HLLVYPSPRHLRREQVFGPVCNDDWKNDDRHDV LCHHYAGGSDELWGRQASHPFSQ*GAIMETGQE HLLHALLDDLWGSVCGPDRPSLWTE*DPRGW*NN PAASLQDRSLDRAGHHGLLPLSGKHLAGQPPHC CL*QYIF*SKIDIQPSLEVSEVSAHHDFP*KAS SAPTTDHLQPHDHDIPAPVLPMEETRERPG*KGL RPETLHNR**AQEST*L*RAMHRRILQRKG*SV QLI***EDTGDFRKGGEHVYAAGGSQRERALHE GFTPDRGHPAGAAGRPYRAHGHGPGAPDRSGAGR VQQNPLEDLVRLHGRRLHCPSEQLQQPGREHLQ APREYRPCR*GDHVPNFSNLNAPYAKPFFLFGQ YERQRWYRKVGKYF*RKVPEPTPGY*FPLCSKRT QSSCSPCQHLGHCS*FQKTIIVYRHLCLCYG*A PL*YRPSGQFREHPWAGRAKLFNSSTFHSPFK* CLCNTCTHRQTSKPEH*F*GHHLHGH*IFFFRLH PPPRMPKPLGLRASDVPHH*AFQK*PLPSHHTL SSRRGSHCEIS*LYVFPLKELLCQLWGACKNSR IHKYYRLY*HKVCQCPSSNCGQSCLPWRSWRQSG GLNLLPSRARSRTESPQL*Q*GE*GQRPQSHHC NILPGG**LRENPVQQHHCSQDRARQQLLGRGA KCAICTHQEELLHQ*QTRQAAEHSKPAKSLPEKQ VLQAGGPRGQPVHEETVQNIGFPKL*KQAQLNL LNIRHRRLKNPALKFSPTPVFPLSLNHTCFILS *AKQAMLWEVLTQR*LLGHRSRKHLI*PSARHRG FKACSHLLGREGEEREKEGLEMNVYPQSQQKAM RAGETRGFEHAPCQEASVDF*PLSRVVGCRAKL QNKIK*PAYTMRYSKLPFCFLFTLAPSLVTDEEH PLYSV*AGSKQFYLTSHILLMPTLL*LIIIKKK QGNSHSSPVSYLFTSGATTVYPFLKKIREQKCL FVLQSK*KKS*C*KNKSQVIYYIQWAFKSSRASS GECN*IILYFLIYFVSHLSWMNSFTEYVILNLK KKKKK

Frame 1

AAAK*KQQELLRGLLLPVPRISAQAQKSWIERA FYKRECVHIIPSTKDPHRCCCGRLIGQHVGLTP SISVLQNEKNESRLSRNDIQSEKWSISKHTQLSP TDAFGTIEFQGGGHSNKAMYVRVSFDTKPDLLL HLMTKEWQLELPKLLISVHGGLQNFELQPKLKQ VFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDAL KDHASKSRGKICTIGIAPWGIVENQEDLIGRDV VRPYQTMSNPMSKLTVLNSMHSHFILADNGTTG KYGAEVKLRRQLEKHISLQKINTRCLPFFSLDSR LFYSFWGSCQLDSVGIGQGVPVVALIVEGGPNV ISIVLEYLRDTPPVPVVVCDGSGRASDILAFGH KYSEEGGLINESLRDQLLVTIQKTFTYTRTQAQH LFIILMECMKKKELITVFRMGSEGHQDIDLAIL TALLKGANASAPDQLSLALAWNRVDIARSQIFI YGQQWPVGSLEQAMLDALVLDRVDFVKLLIENGV SMHRFLTISRLEELYNTRHGPSNTLYHLVRDVK KGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTR KRFRTLYHNLFGPKRPKALKLLGMEDDIPLRRGR KTTKKREEEVDIDLDDPEINHFPFPFHELMVWA VLMKRQKMALFFWQHGEEAMAKALVACKLCKAM AHEASENDMVDDISQELNHNSRDFGQLAVELLDQ SYKQDEQLAMKLLTYELKNWSNATCLQLAVAAK HRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVI LGILLPPSILSLEFKNKDDMPYMSQAQEIHLQEK EAEEPEKPTKEKEEEDMELTAMLGRNNGESSRK KDEEEVQSKHRLIPLGRKIYEFYNAPIVKFWFY TLAYIGYLMLFNYIVLVKMERWPSTQEWIVISYI FTLGIEKMREILMSEPGKLLQKVKVWLQEYWNV TDLIAILLFSVGMILRLQDQPFRSDGRVIYCVN IIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMY FVIIMLVVLMSFGVARQAILFPNEEPSWKLAKN IFYMPYWMIYGEVFADQIDPPCGQNETREDGKI IQLPPCKTGAWIVPAIMACYLLVANILLVNLLIA VFNNTFFEVKSISNQVWKFQRYQLIMTFHERPV LPPPLIIFSHMTMIFQHLCCRWRKHESDPDERD YGLKLFITDDELKKVHDFEEQCIEEYFREKDDRF NSSNDERIRVTSERVENMSMRLEEVNEREHSMK ASLQTVDIRLAQLEDLIGRMATALERLTGLERA ESNKIRSRTSSDCTDAAYIVRQSSFNSQEGNTFK LQESIDPAGEETMSPTSPTLMPRMRSHSFYSVN MKDKGGIEKLESIFKERSLSLHRATSSHSVAKE PKAPAAPANTLAIVPDSRRPSSCIDIYVSAMDEL HCDIDPLDNSVNILGLGEPSFSTPVPSTAPSSS AYATLAPTDRPPSRSIDFEDITSMDTRSFSSDY THLPECQNPWDSEPPMYHTIERSKSSRYLATTPF LLEEAPIVKSHSFMFSPSRSYYANFGVPVKTAE YTSITDCIDTRCVNAPQAIADRAAFPGGLGDKV EDLTCCHPEREAELSHPSSDSEENEAKGRRATIA ISSQEGDNSERTLSNNITVPKIERANSYSAEEP SAPYAHTRKSFSISDKLDRQRNTASLRNPFQRS KSSKPEGRGDSLSMRRLSRTSAFQSFESKHN*TF LISATEGSRIQP*NSLQLQFFPFP*IIPALFLA EQNKQCFGRC*LKGDFWATDQESI*SDPVPDTG DLRHVHTCWAGRGKRGRRKG*R*MCIRSHSRKP* EQGKQGASSTLHARRHLLISDHYQEL*DAGLNC KIK*NSQRTQ*DILNFHSVFFSHWLHHW*LMKS ILFIQYKPAASSST*RPTSFSCQHFCN*SL*RKN KVTVIVHLSLIYSLLVPQLFILF*RK*GNRNAF LYCNRNERRVDVKKTKVK*FIIYSGRSSLVEQA QENVIK*FYIF*FILYLTCHG*IHSLNM*Y*T*K KKKK

Frame 2

LQQNESNRSCSGDCFCQYPESVLRLRNPG*KEH FIKENVSTSYPAPKTPIGVAVGV**ASMLASPP VSPCFRMRKMKVASPEMTSSLKSGPSANTLNSA LRMLLGPLSSKEVAIPTKPCMCEYLLIQNLISSY T**PRNGSWSFPSFSSLSMGACRTLNSSQNSSK SLGKGSSKQQ*QLERGYSLEGLTQVLFVMLAMP *RIMPLSLEERYAP*VLPPGELWKTRRTSLEEML SGHTRPCPIP*ASSLFSTACIPTSFWLTTGPLE NMEQR*NFEDNWKSIFHSRR*TQDACRFSLLTP ACFIHFGVVAS*TQLESVKVFLWWHS*WKEDPM* SRLFWSTFETPLPCQWLSVMGVDGHRTSWPLGI NTQKKAD**MNL*GTSCW*LYRRLSHTLEPKLS ICSSSSWSA*RRRN*LRYFGWDQKDTRTLIWLS* QLYSKEPMPRPQTN*A*L*PGTESTSLAARSLF TGNSGRWDLWSKPCWMP*FWTEWIL*NYS*RME *ACTVFSPSPD*RNCTIRDMGPQIHCTTWSGMSK RGTCPQTTESA*LTSAW*SST*WAGLIAATTRA SASGPSTTTSSAPRGPKP*NCWEWRMIFP*GEE ERQPRNVKKRWTLTWMILRSTTSPSLSMSSWCGL FS*SGRRWPCSSGSTVRRPWPRPWWPASSAKPW LMRPLRTTWLTTFPRS*ITIPETLASWLWSSWT SPTSRTNSWP*NC*RMS*RTGATPRACSLPWLPN TATSSRTRAARCCSPTCGWAGSACARTQASR*F WEFYFLLQFSAWSSRTKTTCPICLRPRKSTSKR RRQKNQRSPQRKKRKRTWSSQQCWDETTGSPPGR RMKRKFRASTG*SPSAEKSMNSTMHPS*SSGST HWRISDT*CSSTISC**RWNAGRPPRNGS*SPI FSPWE*KR*ERF*CQSQGSCYRK*RYGCRSTGMS RTSSPSFCFLSE*SFVSKTSPSGVTGGSSTA*T SFTGISVS*TSSA*TSIWARM***LEK***T*C TLSSLCWWF**ALGSPGKPSFFPMRSHHGNWPRT SSTCPIG*FMGKCLRTR*TLPVDRMRPERMVK* SSCLPARQELGSCRPSWPATS*WQTSCWSTSSL LSLTIHFLK*NRYPTKSGSFRGISSS*LSMKGQF CPHH*SSSAT*P*YSSTCAADGGNTRATRMKGT TA*NSS*PMMSSRKYMTLKSNA*KNTSEKRMIG STHLMMRGYG*LQKGWRTCLCGWRKSTRESTP*R LHSRPWTSGWRSWKTLSGAWPRPWSA*QVWSGP SPTKSARGPRQTARTPPTLSVRAASTARKGTPS SSKRV*TLQVRRPCPQLLQP*CPVCEAILSIRSI *KTKVV*KSWKVFLKKGP*AYTGLLVPTL*QKN PKLLQPLPTPWPLFLIPEDHHRV*TSMSLLWMS STVI*TLWTIP*TSLGWASQAFQLQYLPQPLQVV PMQHLHPQTDLQAGALILRTSPPWTLDLFLQTT PTSQNAKTPGTQSLRCTTPLSVPKVAAT*PPHP FF*KRLPL*NLIALCFPPQGAIMPTLGCL*KQQN TQVLQTVLTQGVSMPLKQLRTELPSLEVLETKW RT*LAAIQSEKQN*VTPALTVRRMRPKAAEPPL QYPPRRVITQREPCPTTSLFPR*SAPTATRQRSQ VRHMHTPGRASPSVTNSTGSGTQQACEIPSREA SPPSRRAEGTACP*GDCPEHRLSKALKASTTKP S*YPPQKAQESSPKILSNSSFSPFLESYLLYS*L SKTSNALGGVNSKVTSGPQIKKAFDLTQCQTQG I*GMFTLAGQGGGREGEGRVRDECVSAVTAESH ESRGNKGLRARSMPGGIC*FLTIIKSCRMQG*IA K*NKIASVHNEIF*TSILFSFHIGSITGD**RA SSLFSISRQQAVLPNVPHPSHANTSVIDHYKEK TR*QS*FTCLLSIHFWCHNCLSFFEENKGTEMPF CIAIEMKEELMLKKQKSSDLLYTVGVQV*SSKL RRM*LNNFIFFNLFCISPVMDEFIH*ICNIELK KKKKK

Sequence NM_001007471

TCCGACCACAGGCTGTTTTGTGCAGGCTGTCCCTCTTCTTCAAAATCGTGCATCCCCTCCCCGAAGCAGCAGGCAGTGTGCCTCCATTCAGCCACATTTG GTATGCATGAGCACGGCTGCAGAGAGAGGGGAGGTGGCTGTTTTAAGAAGGTTCAGGGGCTCAGGCAAGGCTACTTGACTAGTCTTCCAAGTTCCAGGAA GCCTCTGCCCTAATGGAATTTGCAGGTGTGGAGATGACCATGGGATGCCAGAGCCGTGGGGGACCGTTTATTTTCTAGGCATTGCTCAGGTTTTCAGTTT CTTGTTTTCCTGGTGGAATTTGGAAGGGGTCATGAATCAGGCTGATGCTCCTCGACCCCTAAACTGGACCATCCGGAAGCTGTGCCACGCAGCCTTTCTT CCATCTGTCAGACTTCTGAAGGCTCAGAAATCCTGGATAGAAAGAGCATTTTATAAAAGAGAATGTGTCCACATCATACCCAGCACCAAAGACCCCCATA GGTGTTGCTGTGGGCGTCTGATAGGCCAGCATGTTGGCCTCACCCCCAGTATCTCCGTGCTTCAGAATGAGAAAAATGAAAGTCGCCTCTCCCGAAATGA CATCCAGTCTGAAAAGTGGTCCATCAGCAAACACACTCAACTCAGCCCTACGGATGCTTTTGGGACCATTGAGTTCCAAGGAGGTGGCCATTCCAACAAA GCCATGTATGTGCGAGTATCTTTTGATACAAAACCTGATCTCCTCTTACACCTGATGACCAAGGAATGGCAGTTGGAGCTTCCCAAGCTTCTCATCTCTG TCCATGGGGGCCTGCAGAACTTTGAACTCCAGCCAAAACTCAAGCAAGTCTTTGGGAAAGGGCTCATCAAAGCAGCAATGACAACTGGAGCGTGGATATT CACTGGAGGGGTTAACACAGGTGTTATTCGTCATGTTGGCGATGCCTTGAAGGATCATGCCTCTAAGTCTCGAGGAAAGATATGCACCATAGGTATTGCC CCCTGGGGAATTGTGGAAAACCAGGAGGACCTCATTGGAAGAGATGTTGTCCGGCCATACCAGACCATGTCCAATCCCATGAGCAAGCTCACTGTTCTCA ACAGCATGCATTCCCACTTCATTCTGGCTGACAACGGGACCACTGGAAAATATGGAGCAGAGGTGAAACTTCGAAGACAACTGGAAAAGCATATTTCACT CCAGAAGATAAACACAAGAATCGGTCAAGGTGTTCCTGTGGTGGCACTCATAGTGGAAGGAGGACCCAATGTGATCTCGATTGTTTTGGAGTACCTTCGA GACACCCCTCCCGTGCCAGTGGTTGTCTGTGATGGGAGTGGACGGGCATCGGACATCCTGGCCTTTGGGCATAAATACTCAGAAGAAGGCGGACTGATAA ATGAATCTTTGAGGGACCAGCTGTTGGTGACTATACAGAAGACTTTCACATACACTCGAACCCAAGCTCAGCATCTGTTCATCATCCTCATGGAGTGCAT GAAGAAGAAGGAATTGATTACGGTATTTCGGATGGGATCAGAAGGACACCAGGACATTGATTTGGCTATCCTGACAGCTTTACTCAAAGGAGCCAATGCC TCGGCCCCAGACCAACTGAGCTTAGCTTTAGCCTGGAACAGAGTCGACATCGCTCGCAGCCAGATCTTTATTTACGGGCAACAGTGGCCGGTGGGATCTC TGGAGCAAGCCATGTTGGATGCCTTAGTTCTGGACAGAGTGGATTTTGTGAAATTACTCATAGAGAATGGAGTAAGCATGCACCGTTTTCTCACCATCTC CAGACTAGAGGAATTGTACAATACGAGACATGGGCCCTCAAATACATTGTACCACTTGGTCAGGGATGTCAAAAAGGGGAACCTGCCCCCAGACTACAGA ATCAGCCTGATTGACATCGGCCTGGTGATCGAGTACCTGATGGGCGGGGCTTATCGCTGCAACTACACGCGCAAGCGCTTCCGGACCCTCTACCACAACC TCTTCGGCCCCAAGAGGCCCAAAGCCTTGAAACTGCTGGGAATGGAGGATGATATTCCCTTGAGGCGAGGAAGAAAGACAACCAAGAAACGTGAAGAAGA GGTGGACATTGACTTGGATGATCCTGAGATCAACCACTTCCCCTTCCCTTTCCATGAGCTCATGGTGTGGGCTGTTCTCATGAAGCGGCAGAAGATGGCC CTGTTCTTCTGGCAGCACGGTGAGGAGGCCATGGCCAAGGCCCTGGTGGCCTGCAAGCTCTGCAAAGCCATGGCTCATGAGGCCTCTGAGAACGACATGG TTGACGACATTTCCCAGGAGCTGAATCACAATTCCAGAGACTTTGGCCAGCTGGCTGTGGAGCTCCTGGACCAGTCCTACAAGCAGGACGAACAGCTGGC CATGAAACTGCTGACGTATGAGCTGAAGAACTGGAGCAACGCCACGTGCCTGCAGCTTGCCGTGGCTGCCAAACACCGCGACTTCATCGCGCACACGTGC AGCCAGATGCTGCTCACCGACATGTGGATGGGCCGGCTCCGCATGCGCAAGAACTCAGGCCTCAAGGTAATTCTGGGAATTCTACTTCCTCCTTCAATTC TCAGCTTGGAGTTCAAGAACAAAGACGACATGCCCTATATGTCTCAGGCCCAGGAAATCCACCTCCAAGAGAAGGAGGCAGAAGAACCAGAGAAGCCCAC AAAGGAAAAAGAGGAAGAGGACATGGAGCTCACAGCAATGTTGGGACGAAACAACGGGGAGTCCTCCAGGAAGAAGGATGAAGAGGAAGTTCAGAGCAAG CACCGGTTAATCCCCCTCGGCAGAAAAATCTATGAATTCTACAATGCACCCATCGTGAAGTTCTGGTTCTACACACTGGCGTATATCGGATACCTGATGC TCTTCAACTATATCGTGTTAGTGAAGATGGAACGCTGGCCGTCCACCCAGGAATGGATCGTAATCTCCTATATTTTCACCCTGGGAATAGAAAAGATGAG AGAGATTCTGATGTCAGAGCCAGGGAAGTTGCTACAGAAAGTGAAGGTATGGCTGCAGGAGTACTGGAATGTCACGGACCTCATCGCCATCCTTCTGTTT TCTGTCGGAATGATCCTTCGTCTCCAAGACCAGCCCTTCAGGAGTGACGGGAGGGTCATCTACTGCGTGAACATCATTTACTGGTATATCCGTCTCCTAG ACATCTTCGGCGTGAACAAGTATTTGGGCCCGTATGTAATGATGATTGGAAAAATGATGATAGACATGATGTACTTTGTCATCATTATGCTGGTGGTTCT GATGAGCTTTGGGGTCGCCAGGCAAGCCATCCTTTTTCCCAATGAGGAGCCATCATGGAAACTGGCCAAGAACATCTTCTACATGCCCTATTGGATGATT TATGGGGAAGTGTTTGCGGACCAGATAGACCCTCCCTGTGGACAGAATGAGACCCGAGAGGATGGTAAAATAATCCAGCTGCCTCCCTGCAAGACAGGAG CTTGGATCGTGCCGGCCATCATGGCCTGCTACCTCTTAGTGGCAAACATCTTGCTGGTCAACCTCCTCATTGCTGTCTTTAACAATACATTTTTTGAAGT AAAATCGATATCCAACCAAGTCTGGAAGTTTCAGAGGTATCAGCTCATCATGACTTTCCATGAAAGGCCAGTTCTGCCCCCACCACTGATCATCTTCAGC CACATGACCATGATATTCCAGCACCTGTGCTGCCGATGGAGGAAACACGAGAGCGACCCGGATGAAAGGGACTACGGCCTGAAACTCTTCATAACCGATG ATGAGCTCAAGAAAGTACATGACTTTGAAGAGCAATGCATAGAAGAATACTTCAGAGAAAAGGATGATCGGTTCAACTCATCTAATGATGAGAGGATACG GGTGACTTCAGAAAGGGTGGAGAACATGTCTATGCGGCTGGAGGAAGTCAACGAGAGAGAGCACTCCATGAAGGCTTCACTCCAGACCGTGGACATCCGG CTGGCGCAGCTGGAAGACCTTATCGGGCGCATGGCCACGGCCCTGGAGCGCCTGACAGGTCTGGAGCGGGCCGAGTCCAACAAAATCCGCTCGAGGACCT CGTCAGACTGCACGGACGCCGCCTACATTGTCCGTCAGAGCAGCTTCAACAGCCAGGAAGGGAACACCTTCAAGCTCCAAGAGAGTATAGACCCTGCAGG TGAGGAGACCATGTCCCCAACTTCTCCAACCTTAATGCCCCGTATGCGAAGCCATTCTTTCTATTCGGTCAATATGAAAGACAAAGGTGGTATAGAAAAG TTGGAAAGTATTTTTAAAGAAAGGTCCCTGAGCCTACACCGGGCTACTAGTTCCCACTCTGTAGCAAAAGAACCCAAAGCTCCTGCAGCCCCTGCCAACA CCTTGGCCATTGTTCCTGATTCCAGAAGACCATCATCGTGTATAGACATCTATGTCTCTGCTATGGATGAGCTCCACTGTGATATAGACCCTCTGGACAA TTCCGTGAACATCCTTGGGCTGGGCGAGCCAAGCTTTTCAACTCCAGTACCTTCCACAGCCCCTTCAAGTAGTGCCTATGCAACACTTGCACCCACAGAC AGACCTCCAAGCCGGAGCATTGATTTTGAGGACATCACCTCCATGGACACTAGATCTTTTTCTTCAGACTACACCCACCTCCCAGAATGCCAAAACCCCT GGGACTCAGAGCCTCCGATGTACCACACCATTGAGCGTTCCAAAAGTAGCCGCTACCTAGCCACCACACCCTTTCTTCTAGAAGAGGCTCCCATTGTGAA ATCTCATAGCTTTATGTTTTCCCCCTCAAGGAGCTATTATGCCAACTTTGGGGTGCCTGTAAAAACAGCAGAATACACAAGTATTACAGACTGTATTGAC ACAAGGTGTGTCAATGCCCCTCAAGCAATTGCGGACAGAGCTGCCTTCCCTGGAGGTCTTGGAGACAAAGTGGAGGACTTAACTTGCTGCCATCCAGAGC GAGAAGCAGAACTGAGTCACCCCAGCTCTGACAGTGAGGAGAATGAGGCCAAAGGCCGCAGAGCCACCATTGCAATATCCTCCCAGGAGGGTGATAACTC AGAGAGAACCCTGTCCAACAACATCACTGTTCCCAAGATAGAGCGCGCCAACAGCTACTCGGCAGAGGAGCCAAGTGCGCCATATGCACACACCAGGAAG AGCTTCTCCATCAGTGACAAACTCGACAGGCAGCGGAACACAGCAAGCCTGCGAAATCCCTTCCAGAGAAGCAAGTCCTCCAAGCCGGAGGGCCGAGGGG ACAGCCTGTCCATGAGGAGACTGTCCAGAACATCGGCTTTCCAAAGCTTTGAAAGCAAGCACAACTAAACCTTCTTAATATCCGCCACAGAAGGCTCAAG AATCCAGCCCTAAAATTCTCTCCAACTCCAGTTTTTCCCCTTTCCTTGAATCATACCTGCTTTATTCTTAGCTGAGCAAAACAAGCAATGCTTTGGGAGG TGTTAACTCAAAGGTGACTTCTGGGCCACAGATCAAGAAAGCATTTGATCTGACCCAGTGCCAGACACAGGGGATTTAAGGCATGTTCACACTTGCTGGG CAGGGAGGGGGAAGAGAGGGAGAAGGAAGGGTTAGAGATGAATGTGTATCCGCAGTCACAGCAGAAAGCCATGAGAGCAGGGGAAACAAGGGGCTTCGAG CACGCTCCATGCCAGGAGGCATCTGTTGATTTCTGACCATTATCAAGAGTTGTAGGATGCAGGGCTAAATTGCAAAATAAAATAAAATAGCCAGCGTACA CAATGAGATATTCTAAACTTCCATTCTGTTTTCTTTTCACATTGGCTCCATCACTGGTGACTGATGAAGAGCATCCTCTTTATTCAGTATAAGCCGGCAG CAAGCAGTTCTACCTAACGTCCCACATCCTTCTCATGCCAACACTTCTGTAATTGATCATTATAAAGAAAAAACAAGGTAACAGTCATAGTTCACCTGTC TCTTATCTATTCACTTCTGGTGCCACAACTGTTTATCCTTTTTTGAAGAAAATAAGGGAACAGAAATGCCTTTTTGTATTGCAATCGAAATGAAAGAAGA GTTGATGTTAAAAAAACAAAAGTCAAGTGATTTATTATATACAGTGGGCGTTCAAGTCTAGTCGAGCAAGCTCAGGAGAATGTAATTAAATAATTTTATA TTTTTTAATTTATTTTGTATCTCACCTGTCATGGATGAATTCATTCACTGAATATGTAATATTGAACTTAAAAAAAAAAAAAAAAAAA

refseq_TRPM3.F2 (forward primer)
refseq_TRPM3.R2 (reverse primer)
Single exon skipping

Frame 0

SDHRLFCAGCPSSSKSCIPSPKQQAVCLHSATF GMHEHGCRERGGGCFKKVQGLRQGYLTSLPSSRK PLP*WNLQVWR*PWDARAVGDRLFSRHCSGFQF LVFLVEFGRGHESG*CSSTPKLDHPEAVPRSLS SICQTSEGSEILDRKSIL*KRMCPHHTQHQRPP* VLLWASDRPACWPHPQYLRASE*EK*KSPLPK* HPV*KVVHQQTHSTQPYGCFWDH*VPRRWPFQQ SHVCASIF*YKT*SPLTPDDQGMAVGASQASHLC PWGPAEL*TPAKTQASLWERAHQSSNDNWSVDI HWRG*HRCYSSCWRCLEGSCL*VSRKDMHHRYC PLGNCGKPGGPHWKRCCPAIPDHVQSHEQAHCSQ QHAFPLHSG*QRDHWKIWSRGETSKTTGKAYFT PEDKHKNRSRCSCGGTHSGRRTQCDLDCFGVPS RHPSRASGCL*WEWTGIGHPGLWA*ILRRRRTDK *IFEGPAVGDYTEDFHIHSNPSSASVHHPHGVH EEEGIDYGISDGIRRTPGH*FGYPDSFTQRSQC LGPRPTELSFSLEQSRHRSQPDLYLRATVAGGIS GASHVGCLSSGQSGFCEITHREWSKHAPFSHHL QTRGIVQYETWALKYIVPLGQGCQKGEPAPRLQ NQPD*HRPGDRVPDGRGLSLQLHAQALPDPLPQP LRPQEAQSLETAGNGG*YSLEARKKDNQET*RR GGH*LG*S*DQPLPLPFP*AHGVGCSHEAAEDG PVLLAAR*GGHGQGPGGLQALQSHGS*GL*ERHG *RHFPGAESQFQRLWPAGCGAPGPVLQAGRTAG HETADV*AEELEQRHVPAACRGCQTPRLHRAHV QPDAAHRHVDGPAPHAQELRPQGNSGNSTSSFNS QLGVQEQRRHALYVSGPGNPPPREGGRRTREAH KGKRGRGHGAHSNVGTKQRGVLQEEG*RGSSEQ APVNPPRQKNL*ILQCTHREVLVLHTGVYRIPDA LQLYRVSEDGTLAVHPGMDRNLLYFHPGNRKDE RDSDVRAREVATESEGMAAGVLECHGPHRHPSV FCRNDPSSPRPALQE*REGHLLREHHLLVYPSPR HLRREQVFGPVCNDDWKNDDRHDVLCHHYAGGS DELWGRQASHPFSQ*GAIMETGQEHLLHALLDD LWGSVCGPDRPSLWTE*DPRGW*NNPAASLQDRS LDRAGHHGLLPLSGKHLAGQPPHCCL*QYIF*S KIDIQPSLEVSEVSAHHDFP*KASSAPTTDHLQ PHDHDIPAPVLPMEETRERPG*KGLRPETLHNR* *AQEST*L*RAMHRRILQRKG*SVQLI***EDT GDFRKGGEHVYAAGGSQRERALHEGFTPDRGHP AGAAGRPYRAHGHGPGAPDRSGAGRVQQNPLEDL VRLHGRRLHCPSEQLQQPGREHLQAPREYRPCR *GDHVPNFSNLNAPYAKPFFLFGQYERQRWYRK VGKYF*RKVPEPTPGY*FPLCSKRTQSSCSPCQH LGHCS*FQKTIIVYRHLCLCYG*APL*YRPSGQ FREHPWAGRAKLFNSSTFHSPFK*CLCNTCTHR QTSKPEH*F*GHHLHGH*IFFFRLHPPPRMPKPL GLRASDVPHH*AFQK*PLPSHHTLSSRRGSHCE IS*LYVFPLKELLCQLWGACKNSRIHKYYRLY* HKVCQCPSSNCGQSCLPWRSWRQSGGLNLLPSRA RSRTESPQL*Q*GE*GQRPQSHHCNILPGG**L RENPVQQHHCSQDRARQQLLGRGAKCAICTHQE ELLHQ*QTRQAAEHSKPAKSLPEKQVLQAGGPRG QPVHEETVQNIGFPKL*KQAQLNLLNIRHRRLK NPALKFSPTPVFPLSLNHTCFILS*AKQAMLWE VLTQR*LLGHRSRKHLI*PSARHRGFKACSHLLG REGEEREKEGLEMNVYPQSQQKAMRAGETRGFE HAPCQEASVDF*PLSRVVGCRAKLQNKIK*PAY TMRYSKLPFCFLFTLAPSLVTDEEHPLYSV*AGS KQFYLTSHILLMPTLL*LIIIKKKQGNSHSSPV SYLFTSGATTVYPFLKKIREQKCLFVLQSK*KK S*C*KNKSQVIYYIQWAFKSSRASSGECN*IILY FLIYFVSHLSWMNSFTEYVILNLKKKKKK

Frame 1

PTTGCFVQAVPLLQNRASPPRSSRQCASIQPHL VCMSTAAERGEVAVLRRFRGSGKAT*LVFQVPG SLCPNGICRCGDDHGMPEPWGTVYFLGIAQVFSF LFSWWNLEGVMNQADAPRPLNWTIRKLCHAAFL PSVRLLKAQKSWIERAFYKRECVHIIPSTKDPH RCCCGRLIGQHVGLTPSISVLQNEKNESRLSRND IQSEKWSISKHTQLSPTDAFGTIEFQGGGHSNK AMYVRVSFDTKPDLLLHLMTKEWQLELPKLLIS VHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIF TGGVNTGVIRHVGDALKDHASKSRGKICTIGIA PWGIVENQEDLIGRDVVRPYQTMSNPMSKLTVL NSMHSHFILADNGTTGKYGAEVKLRRQLEKHISL QKINTRIGQGVPVVALIVEGGPNVISIVLEYLR DTPPVPVVVCDGSGRASDILAFGHKYSEEGGLI NESLRDQLLVTIQKTFTYTRTQAQHLFIILMECM KKKELITVFRMGSEGHQDIDLAILTALLKGANA SAPDQLSLALAWNRVDIARSQIFIYGQQWPVGS LEQAMLDALVLDRVDFVKLLIENGVSMHRFLTIS RLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYR ISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHN LFGPKRPKALKLLGMEDDIPLRRGRKTTKKREEE VDIDLDDPEINHFPFPFHELMVWAVLMKRQKMA LFFWQHGEEAMAKALVACKLCKAMAHEASENDM VDDISQELNHNSRDFGQLAVELLDQSYKQDEQLA MKLLTYELKNWSNATCLQLAVAAKHRDFIAHTC SQMLLTDMWMGRLRMRKNSGLKVILGILLPPSI LSLEFKNKDDMPYMSQAQEIHLQEKEAEEPEKPT KEKEEEDMELTAMLGRNNGESSRKKDEEEVQSK HRLIPLGRKIYEFYNAPIVKFWFYTLAYIGYLM LFNYIVLVKMERWPSTQEWIVISYIFTLGIEKMR EILMSEPGKLLQKVKVWLQEYWNVTDLIAILLF SVGMILRLQDQPFRSDGRVIYCVNIIYWYIRLL DIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVL MSFGVARQAILFPNEEPSWKLAKNIFYMPYWMI YGEVFADQIDPPCGQNETREDGKIIQLPPCKTG AWIVPAIMACYLLVANILLVNLLIAVFNNTFFEV KSISNQVWKFQRYQLIMTFHERPVLPPPLIIFS HMTMIFQHLCCRWRKHESDPDERDYGLKLFITD DELKKVHDFEEQCIEEYFREKDDRFNSSNDERIR VTSERVENMSMRLEEVNEREHSMKASLQTVDIR LAQLEDLIGRMATALERLTGLERAESNKIRSRT SSDCTDAAYIVRQSSFNSQEGNTFKLQESIDPAG EETMSPTSPTLMPRMRSHSFYSVNMKDKGGIEK LESIFKERSLSLHRATSSHSVAKEPKAPAAPAN TLAIVPDSRRPSSCIDIYVSAMDELHCDIDPLDN SVNILGLGEPSFSTPVPSTAPSSSAYATLAPTD RPPSRSIDFEDITSMDTRSFSSDYTHLPECQNP WDSEPPMYHTIERSKSSRYLATTPFLLEEAPIVK SHSFMFSPSRSYYANFGVPVKTAEYTSITDCID TRCVNAPQAIADRAAFPGGLGDKVEDLTCCHPE REAELSHPSSDSEENEAKGRRATIAISSQEGDNS ERTLSNNITVPKIERANSYSAEEPSAPYAHTRK SFSISDKLDRQRNTASLRNPFQRSKSSKPEGRG DSLSMRRLSRTSAFQSFESKHN*TFLISATEGSR IQP*NSLQLQFFPFP*IIPALFLAEQNKQCFGR C*LKGDFWATDQESI*SDPVPDTGDLRHVHTCW AGRGKRGRRKG*R*MCIRSHSRKP*EQGKQGASS TLHARRHLLISDHYQEL*DAGLNCKIK*NSQRT Q*DILNFHSVFFSHWLHHW*LMKSILFIQYKPA ASSST*RPTSFSCQHFCN*SL*RKNKVTVIVHLS LIYSLLVPQLFILF*RK*GNRNAFLYCNRNERR VDVKKTKVK*FIIYSGRSSLVEQAQENVIK*FY IF*FILYLTCHG*IHSLNM*Y*T*KKKKK

Frame 2

RPQAVLCRLSLFFKIVHPLPEAAGSVPPFSHIW YA*ARLQREGRWLF*EGSGAQARLLD*SSKFQE ASALMEFAGVEMTMGCQSRGGPFIF*ALLRFSV SCFPGGIWKGS*IRLMLLDP*TGPSGSCATQPFF HLSDF*RLRNPG*KEHFIKENVSTSYPAPKTPI GVAVGV**ASMLASPPVSPCFRMRKMKVASPEM TSSLKSGPSANTLNSALRMLLGPLSSKEVAIPTK PCMCEYLLIQNLISSYT**PRNGSWSFPSFSSL SMGACRTLNSSQNSSKSLGKGSSKQQ*QLERGY SLEGLTQVLFVMLAMP*RIMPLSLEERYAP*VLP PGELWKTRRTSLEEMLSGHTRPCPIP*ASSLFS TACIPTSFWLTTGPLENMEQR*NFEDNWKSIFH SRR*TQESVKVFLWWHS*WKEDPM*SRLFWSTFE TPLPCQWLSVMGVDGHRTSWPLGINTQKKAD** MNL*GTSCW*LYRRLSHTLEPKLSICSSSSWSA *RRRN*LRYFGWDQKDTRTLIWLS*QLYSKEPMP RPQTN*A*L*PGTESTSLAARSLFTGNSGRWDL WSKPCWMP*FWTEWIL*NYS*RME*ACTVFSPS PD*RNCTIRDMGPQIHCTTWSGMSKRGTCPQTTE SA*LTSAW*SST*WAGLIAATTRASASGPSTTT SSAPRGPKP*NCWEWRMIFP*GEEERQPRNVKK RWTLTWMILRSTTSPSLSMSSWCGLFS*SGRRWP CSSGSTVRRPWPRPWWPASSAKPWLMRPLRTTW LTTFPRS*ITIPETLASWLWSSWTSPTSRTNSW P*NC*RMS*RTGATPRACSLPWLPNTATSSRTRA ARCCSPTCGWAGSACARTQASR*FWEFYFLLQF SAWSSRTKTTCPICLRPRKSTSKRRRQKNQRSP QRKKRKRTWSSQQCWDETTGSPPGRRMKRKFRAS TG*SPSAEKSMNSTMHPS*SSGSTHWRISDT*C SSTISC**RWNAGRPPRNGS*SPIFSPWE*KR* ERF*CQSQGSCYRK*RYGCRSTGMSRTSSPSFCF LSE*SFVSKTSPSGVTGGSSTA*TSFTGISVS* TSSA*TSIWARM***LEK***T*CTLSSLCWWF **ALGSPGKPSFFPMRSHHGNWPRTSSTCPIG*F MGKCLRTR*TLPVDRMRPERMVK*SSCLPARQE LGSCRPSWPATS*WQTSCWSTSSLLSLTIHFLK *NRYPTKSGSFRGISSS*LSMKGQFCPHH*SSSA T*P*YSSTCAADGGNTRATRMKGTTA*NSS*PM MSSRKYMTLKSNA*KNTSEKRMIGSTHLMMRGY G*LQKGWRTCLCGWRKSTRESTP*RLHSRPWTSG WRSWKTLSGAWPRPWSA*QVWSGPSPTKSARGP RQTARTPPTLSVRAASTARKGTPSSSKRV*TLQ VRRPCPQLLQP*CPVCEAILSIRSI*KTKVV*KS WKVFLKKGP*AYTGLLVPTL*QKNPKLLQPLPT PWPLFLIPEDHHRV*TSMSLLWMSSTVI*TLWT IP*TSLGWASQAFQLQYLPQPLQVVPMQHLHPQT DLQAGALILRTSPPWTLDLFLQTTPTSQNAKTP GTQSLRCTTPLSVPKVAAT*PPHPFF*KRLPL* NLIALCFPPQGAIMPTLGCL*KQQNTQVLQTVLT QGVSMPLKQLRTELPSLEVLETKWRT*LAAIQS EKQN*VTPALTVRRMRPKAAEPPLQYPPRRVIT QREPCPTTSLFPR*SAPTATRQRSQVRHMHTPGR ASPSVTNSTGSGTQQACEIPSREASPPSRRAEG TACP*GDCPEHRLSKALKASTTKPS*YPPQKAQ ESSPKILSNSSFSPFLESYLLYS*LSKTSNALGG VNSKVTSGPQIKKAFDLTQCQTQGI*GMFTLAG QGGGREGEGRVRDECVSAVTAESHESRGNKGLR ARSMPGGIC*FLTIIKSCRMQG*IAK*NKIASVH NEIF*TSILFSFHIGSITGD**RASSLFSISRQ QAVLPNVPHPSHANTSVIDHYKEKTR*QS*FTC LLSIHFWCHNCLSFFEENKGTEMPFCIAIEMKEE LMLKKQKSSDLLYTVGVQV*SSKLRRM*LNNFI FFNLFCISPVMDEFIH*ICNIELKKKKKK

Sequence NM_020952

AGCTGCAGCAAAATGAAAGCAACAGGAGCTGCTCCGGGGACTGCTTTTGCCAGTACCCAGAATCAGTGCTCAGGCTCAGAAATCCTGGATAGAAAGAGCA TTTTATAAAAGAGAATGTGTCCACATCATACCCAGCACCAAAGACCCCCATAGGTGTTGCTGTGGGCGTCTGATAGGCCAGCATGTTGGCCTCACCCCCA GTATCTCCGTGCTTCAGAATGAGAAAAATGAAAGTCGCCTCTCCCGAAATGACATCCAGTCTGAAAAGTGGTCCATCAGCAAACACACTCAACTCAGCCC TACGGATGCTTTTGGGACCATTGAGTTCCAAGGAGGTGGCCATTCCAACAAAGCCATGTATGTGCGAGTATCTTTTGATACAAAACCTGATCTCCTCTTA CACCTGATGACCAAGGAATGGCAGTTGGAGCTTCCCAAGCTTCTCATCTCTGTCCATGGGGGCCTGCAGAACTTTGAACTCCAGCCAAAACTCAAGCAAG TCTTTGGGAAAGGGCTCATCAAAGCAGCAATGACAACTGGAGCGTGGATATTCACTGGAGGGGTTAACACAGGTGTTATTCGTCATGTTGGCGATGCCTT GAAGGATCATGCCTCTAAGTCTCGAGGAAAGATATGCACCATAGGTATTGCCCCCTGGGGAATTGTGGAAAACCAGGAGGACCTCATTGGAAGAGATGTT GTCCGGCCATACCAGACCATGTCCAATCCCATGAGCAAGCTCACTGTTCTCAACAGCATGCATTCCCACTTCATTCTGGCTGACAACGGGACCACTGGAA AATATGGAGCAGAGGTGAAACTTCGAAGACAACTGGAAAAGCATATTTCACTCCAGAAGATAAACACAAGAATCGGTCAAGGTGTTCCTGTGGTGGCACT CATAGTGGAAGGAGGACCCAATGTGATCTCGATTGTTTTGGAGTACCTTCGAGACACCCCTCCCGTGCCAGTGGTTGTCTGTGATGGGAGTGGACGGGCA TCGGACATCCTGGCCTTTGGGCATAAATACTCAGAAGAAGGCGGACTGATAAATGAATCTTTGAGGGACCAGCTGTTGGTGACTATACAGAAGACTTTCA CATACACTCGAACCCAAGCTCAGCATCTGTTCATCATCCTCATGGAGTGCATGAAGAAGAAGGAATTGATTACGGTATTTCGGATGGGATCAGAAGGACA CCAGGACATTGATTTGGCTATCCTGACAGCTTTACTCAAAGGAGCCAATGCCTCGGCCCCAGACCAACTGAGCTTAGCTTTAGCCTGGAACAGAGTCGAC ATCGCTCGCAGCCAGATCTTTATTTACGGGCAACAGTGGCCGGTGGGATCTCTGGAGCAAGCCATGTTGGATGCCTTAGTTCTGGACAGAGTGGATTTTG TGAAATTACTCATAGAGAATGGAGTAAGCATGCACCGTTTTCTCACCATCTCCAGACTAGAGGAATTGTACAATACGAGACATGGGCCCTCAAATACATT GTACCACTTGGTCAGGGATGTCAAAAAGGGGAACCTGCCCCCAGACTACAGAATCAGCCTGATTGACATCGGCCTGGTGATCGAGTACCTGATGGGCGGG GCTTATCGCTGCAACTACACGCGCAAGCGCTTCCGGACCCTCTACCACAACCTCTTCGGCCCCAAGAGGCCCAAAGCCTTGAAACTGCTGGGAATGGAGG ATGATATTCCCTTGAGGCGAGGAAGAAAGACAACCAAGAAACGTGAAGAAGAGGTGGACATTGACTTGGATGATCCTGAGATCAACCACTTCCCCTTCCC TTTCCATGAGCTCATGGTGTGGGCTGTTCTCATGAAGCGGCAGAAGATGGCCCTGTTCTTCTGGCAGCACGGTGAGGAGGCCATGGCCAAGGCCCTGGTG GCCTGCAAGCTCTGCAAAGCCATGGCTCATGAGGCCTCTGAGAACGACATGGTTGACGACATTTCCCAGGAGCTGAATCACAATTCCAGAGACTTTGGCC AGCTGGCTGTGGAGCTCCTGGACCAGTCCTACAAGCAGGACGAACAGCTGGCCATGAAACTGCTGACGTATGAGCTGAAGAACTGGAGCAACGCCACGTG CCTGCAGCTTGCCGTGGCTGCCAAACACCGCGACTTCATCGCGCACACGTGCAGCCAGATGCTGCTCACCGACATGTGGATGGGCCGGCTCCGCATGCGC AAGAACTCAGGCCTCAAGGTAATTCTGGGAATTCTACTTCCTCCTTCAATTCTCAGCTTGGAGTTCAAGAACAAAGACGACATGCCCTATATGTCTCAGG CCCAGGAAATCCACCTCCAAGAGAAGGAGGCAGAAGAACCAGAGAAGCCCACAAAGGAAAAAGAGGAAGAGGACATGGAGCTCACAGCAATGTTGGGACG AAACAACGGGGAGTCCTCCAGGAAGAAGGATGAAGAGGAAGTTCAGAGCAAGCACCGGTTAATCCCCCTCGGCAGAAAAATCTATGAATTCTACAATGCA CCCATCGTGAAGTTCTGGTTCTACACACTGGCGTATATCGGATACCTGATGCTCTTCAACTATATCGTGTTAGTGAAGATGGAACGCTGGCCGTCCACCC AGGAATGGATCGTAATCTCCTATATTTTCACCCTGGGAATAGAAAAGATGAGAGAGATTCTGATGTCAGAGCCAGGGAAGTTGCTACAGAAAGTGAAGGT ATGGCTGCAGGAGTACTGGAATGTCACGGACCTCATCGCCATCCTTCTGTTTTCTGTCGGAATGATCCTTCGTCTCCAAGACCAGCCCTTCAGGAGTGAC GGGAGGGTCATCTACTGCGTGAACATCATTTACTGGTATATCCGTCTCCTAGACATCTTCGGCGTGAACAAGTATTTGGGCCCGTATGTAATGATGATTG GAAAAATGATGATAGACATGATGTACTTTGTCATCATTATGCTGGTGGTTCTGATGAGCTTTGGGGTCGCCAGGCAAGCCATCCTTTTTCCCAATGAGGA GCCATCATGGAAACTGGCCAAGAACATCTTCTACATGCCCTATTGGATGATTTATGGGGAAGTGTTTGCGGACCAGATAGACCCTCCCTGTGGACAGAAT GAGACCCGAGAGGATGGTAAAATAATCCAGCTGCCTCCCTGCAAGACAGGAGCTTGGATCGTGCCGGCCATCATGGCCTGCTACCTCTTAGTGGCAAACA TCTTGCTGGTCAACCTCCTCATTGCTGTCTTTAACAATACATTTTTTGAAGTAAAATCGATATCCAACCAAGTCTGGAAGTTTCAGAGGTATCAGCTCAT CATGACTTTCCATGAAAGGCCAGTTCTGCCCCCACCACTGATCATCTTCAGCCACATGACCATGATATTCCAGCACCTGTGCTGCCGATGGAGGAAACAC GAGAGCGACCCGGATGAAAGGGACTACGGCCTGAAACTCTTCATAACCGATGATGAGCTCAAGAAAGTACATGACTTTGAAGAGCAATGCATAGAAGAAT ACTTCAGAGAAAAGGATGATCGGTTCAACTCATCTAATGATGAGAGGATACGGGTGACTTCAGAAAGGGTGGAGAACATGTCTATGCGGCTGGAGGAAGT CAACGAGAGAGAGCACTCCATGAAGGCTTCACTCCAGACCGTGGACATCCGGCTGGCGCAGCTGGAAGACCTTATCGGGCGCATGGCCACGGCCCTGGAG CGCCTGACAGGTCTGGAGCGGGCCGAGTCCAACAAAATCCGCTCGAGGACCTCGTCAGACTGCACGGACGCCGCCTACATTGTCCGTCAGAGCAGCTTCA ACAGCCAGGAAGGGAACACCTTCAAGCTCCAAGAGAGTATAGACCCTGCAGGTGAGGAGACCATGTCCCCAACTTCTCCAACCTTAATGCCCCGTATGCG AAGCCATTCTTTCTATTCGGTCAATATGAAAGACAAAGGTGGTATAGAAAAGTTGGAAAGTATTTTTAAAGAAAGGTCCCTGAGCCTACACCGGGCTACT AGTTCCCACTCTGTAGCAAAAGAACCCAAAGCTCCTGCAGCCCCTGCCAACACCTTGGCCATTGTTCCTGATTCCAGAAGACCATCATCGTGTATAGACA TCTATGTCTCTGCTATGGATGAGCTCCACTGTGATATAGACCCTCTGGACAATTCCGTGAACATCCTTGGGCTGGGCGAGCCAAGCTTTTCAACTCCAGT ACCTTCCACAGCCCCTTCAAGTAGTGCCTATGCAACACTTGCACCCACAGACAGACCTCCAAGCCGGAGCATTGATTTTGAGGACATCACCTCCATGGAC ACTAGATCTTTTTCTTCAGACTACACCCACCTCCCAGAATGCCAAAACCCCTGGGACTCAGAGCCTCCGATGTACCACACCATTGAGCGTTCCAAAAGTA GCCGCTACCTAGCCACCACACCCTTTCTTCTAGAAGAGGCTCCCATTGTGAAATCTCATAGCTTTATGTTTTCCCCCTCAAGGAGCTATTATGCCAACTT TGGGGTGCCTGTAAAAACAGCAGAATACACAAGTATTACAGACTGTATTGACACAAGGTGTGTCAATGCCCCTCAAGCAATTGCGGACAGAGCTGCCTTC CCTGGAGGTCTTGGAGACAAAGTGGAGGACTTAACTTGCTGCCATCCAGAGCGAGAAGCAGAACTGAGTCACCCCAGCTCTGACAGTGAGGAGAATGAGG CCAAAGGCCGCAGAGCCACCATTGCAATATCCTCCCAGGAGGGTGATAACTCAGAGAGAACCCTGTCCAACAACATCACTGTTCCCAAGATAGAGCGCGC CAACAGCTACTCGGCAGAGGAGCCAAGTGCGCCATATGCACACACCAGGAAGAGCTTCTCCATCAGTGACAAACTCGACAGGCAGCGGAACACAGCAAGC CTGCGAAATCCCTTCCAGAGAAGCAAGTCCTCCAAGCCGGAGGGCCGAGGGGACAGCCTGTCCATGAGGAGACTGTCCAGAACATCGGCTTTCCAAAGCT TTGAAAGCAAGCACAACTAAACCTTCTTAATATCCGCCACAGAAGGCTCAAGAATCCAGCCCTAAAATTCTCTCCAACTCCAGTTTTTCCCCTTTCCTTG AATCATACCTGCTTTATTCTTAGCTGAGCAAAACAAGCAATGCTTTGGGAGGTGTTAACTCAAAGGTGACTTCTGGGCCACAGATCAAGAAAGCATTTGA TCTGACCCAGTGCCAGACACAGGGGATTTAAGGCATGTTCACACTTGCTGGGCAGGGAGGGGGAAGAGAGGGAGAAGGAAGGGTTAGAGATGAATGTGTA TCCGCAGTCACAGCAGAAAGCCATGAGAGCAGGGGAAACAAGGGGCTTCGAGCACGCTCCATGCCAGGAGGCATCTGTTGATTTCTGACCATTATCAAGA GTTGTAGGATGCAGGGCTAAATTGCAAAATAAAATAAAATAGCCAGCGTACACAATGAGATATTCTAAACTTCCATTCTGTTTTCTTTTCACATTGGCTC CATCACTGGTGACTGATGAAGAGCATCCTCTTTATTCAGTATAAGCCGGCAGCAAGCAGTTCTACCTAACGTCCCACATCCTTCTCATGCCAACACTTCT GTAATTGATCATTATAAAGAAAAAACAAGGTAACAGTCATAGTTCACCTGTCTCTTATCTATTCACTTCTGGTGCCACAACTGTTTATCCTTTTTTGAAG AAAATAAGGGAACAGAAATGCCTTTTTGTATTGCAATCGAAATGAAAGAAGAGTTGATGTTAAAAAAACAAAAGTCAAGTGATTTATTATATACAGTGGG CGTTCAAGTCTAGTCGAGCAAGCTCAGGAGAATGTAATTAAATAATTTTATATTTTTTAATTTATTTTGTATCTCACCTGTCATGGATGAATTCATTCAC TGAATATGTAATATTGAACTTAAAAAAAAAAAAAAAAAAA

refseq_TRPM3.F2 (forward primer)
refseq_TRPM3.R2 (reverse primer)
Single exon skipping

Frame 0

SCSKMKATGAAPGTAFASTQNQCSGSEILDRKS IL*KRMCPHHTQHQRPP*VLLWASDRPACWPHPQ YLRASE*EK*KSPLPK*HPV*KVVHQQTHSTQP YGCFWDH*VPRRWPFQQSHVCASIF*YKT*SPL TPDDQGMAVGASQASHLCPWGPAEL*TPAKTQAS LWERAHQSSNDNWSVDIHWRG*HRCYSSCWRCL EGSCL*VSRKDMHHRYCPLGNCGKPGGPHWKRC CPAIPDHVQSHEQAHCSQQHAFPLHSG*QRDHWK IWSRGETSKTTGKAYFTPEDKHKNRSRCSCGGT HSGRRTQCDLDCFGVPSRHPSRASGCL*WEWTG IGHPGLWA*ILRRRRTDK*IFEGPAVGDYTEDFH IHSNPSSASVHHPHGVHEEEGIDYGISDGIRRT PGH*FGYPDSFTQRSQCLGPRPTELSFSLEQSR HRSQPDLYLRATVAGGISGASHVGCLSSGQSGFC EITHREWSKHAPFSHHLQTRGIVQYETWALKYI VPLGQGCQKGEPAPRLQNQPD*HRPGDRVPDGR GLSLQLHAQALPDPLPQPLRPQEAQSLETAGNGG *YSLEARKKDNQET*RRGGH*LG*S*DQPLPLP FP*AHGVGCSHEAAEDGPVLLAAR*GGHGQGPG GLQALQSHGS*GL*ERHG*RHFPGAESQFQRLWP AGCGAPGPVLQAGRTAGHETADV*AEELEQRHV PAACRGCQTPRLHRAHVQPDAAHRHVDGPAPHA QELRPQGNSGNSTSSFNSQLGVQEQRRHALYVSG PGNPPPREGGRRTREAHKGKRGRGHGAHSNVGT KQRGVLQEEG*RGSSEQAPVNPPRQKNL*ILQC THREVLVLHTGVYRIPDALQLYRVSEDGTLAVHP GMDRNLLYFHPGNRKDERDSDVRAREVATESEG MAAGVLECHGPHRHPSVFCRNDPSSPRPALQE* REGHLLREHHLLVYPSPRHLRREQVFGPVCNDDW KNDDRHDVLCHHYAGGSDELWGRQASHPFSQ*G AIMETGQEHLLHALLDDLWGSVCGPDRPSLWTE *DPRGW*NNPAASLQDRSLDRAGHHGLLPLSGKH LAGQPPHCCL*QYIF*SKIDIQPSLEVSEVSAH HDFP*KASSAPTTDHLQPHDHDIPAPVLPMEET RERPG*KGLRPETLHNR**AQEST*L*RAMHRRI LQRKG*SVQLI***EDTGDFRKGGEHVYAAGGS QRERALHEGFTPDRGHPAGAAGRPYRAHGHGPG APDRSGAGRVQQNPLEDLVRLHGRRLHCPSEQLQ QPGREHLQAPREYRPCR*GDHVPNFSNLNAPYA KPFFLFGQYERQRWYRKVGKYF*RKVPEPTPGY *FPLCSKRTQSSCSPCQHLGHCS*FQKTIIVYRH LCLCYG*APL*YRPSGQFREHPWAGRAKLFNSS TFHSPFK*CLCNTCTHRQTSKPEH*F*GHHLHG H*IFFFRLHPPPRMPKPLGLRASDVPHH*AFQK* PLPSHHTLSSRRGSHCEIS*LYVFPLKELLCQL WGACKNSRIHKYYRLY*HKVCQCPSSNCGQSCL PWRSWRQSGGLNLLPSRARSRTESPQL*Q*GE*G QRPQSHHCNILPGG**LRENPVQQHHCSQDRAR QQLLGRGAKCAICTHQEELLHQ*QTRQAAEHSK PAKSLPEKQVLQAGGPRGQPVHEETVQNIGFPKL *KQAQLNLLNIRHRRLKNPALKFSPTPVFPLSL NHTCFILS*AKQAMLWEVLTQR*LLGHRSRKHL I*PSARHRGFKACSHLLGREGEEREKEGLEMNVY PQSQQKAMRAGETRGFEHAPCQEASVDF*PLSR VVGCRAKLQNKIK*PAYTMRYSKLPFCFLFTLA PSLVTDEEHPLYSV*AGSKQFYLTSHILLMPTLL *LIIIKKKQGNSHSSPVSYLFTSGATTVYPFLK KIREQKCLFVLQSK*KKS*C*KNKSQVIYYIQW AFKSSRASSGECN*IILYFLIYFVSHLSWMNSFT EYVILNLKKKKKK

Frame 1

AAAK*KQQELLRGLLLPVPRISAQAQKSWIERA FYKRECVHIIPSTKDPHRCCCGRLIGQHVGLTP SISVLQNEKNESRLSRNDIQSEKWSISKHTQLSP TDAFGTIEFQGGGHSNKAMYVRVSFDTKPDLLL HLMTKEWQLELPKLLISVHGGLQNFELQPKLKQ VFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDAL KDHASKSRGKICTIGIAPWGIVENQEDLIGRDV VRPYQTMSNPMSKLTVLNSMHSHFILADNGTTG KYGAEVKLRRQLEKHISLQKINTRIGQGVPVVAL IVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRA SDILAFGHKYSEEGGLINESLRDQLLVTIQKTF TYTRTQAQHLFIILMECMKKKELITVFRMGSEGH QDIDLAILTALLKGANASAPDQLSLALAWNRVD IARSQIFIYGQQWPVGSLEQAMLDALVLDRVDF VKLLIENGVSMHRFLTISRLEELYNTRHGPSNTL YHLVRDVKKGNLPPDYRISLIDIGLVIEYLMGG AYRCNYTRKRFRTLYHNLFGPKRPKALKLLGME DDIPLRRGRKTTKKREEEVDIDLDDPEINHFPFP FHELMVWAVLMKRQKMALFFWQHGEEAMAKALV ACKLCKAMAHEASENDMVDDISQELNHNSRDFG QLAVELLDQSYKQDEQLAMKLLTYELKNWSNATC LQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMR KNSGLKVILGILLPPSILSLEFKNKDDMPYMSQ AQEIHLQEKEAEEPEKPTKEKEEEDMELTAMLGR NNGESSRKKDEEEVQSKHRLIPLGRKIYEFYNA PIVKFWFYTLAYIGYLMLFNYIVLVKMERWPST QEWIVISYIFTLGIEKMREILMSEPGKLLQKVKV WLQEYWNVTDLIAILLFSVGMILRLQDQPFRSD GRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMI GKMMIDMMYFVIIMLVVLMSFGVARQAILFPNEE PSWKLAKNIFYMPYWMIYGEVFADQIDPPCGQN ETREDGKIIQLPPCKTGAWIVPAIMACYLLVAN ILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLI MTFHERPVLPPPLIIFSHMTMIFQHLCCRWRKH ESDPDERDYGLKLFITDDELKKVHDFEEQCIEE YFREKDDRFNSSNDERIRVTSERVENMSMRLEEV NEREHSMKASLQTVDIRLAQLEDLIGRMATALE RLTGLERAESNKIRSRTSSDCTDAAYIVRQSSF NSQEGNTFKLQESIDPAGEETMSPTSPTLMPRMR SHSFYSVNMKDKGGIEKLESIFKERSLSLHRAT SSHSVAKEPKAPAAPANTLAIVPDSRRPSSCID IYVSAMDELHCDIDPLDNSVNILGLGEPSFSTPV PSTAPSSSAYATLAPTDRPPSRSIDFEDITSMD TRSFSSDYTHLPECQNPWDSEPPMYHTIERSKS SRYLATTPFLLEEAPIVKSHSFMFSPSRSYYANF GVPVKTAEYTSITDCIDTRCVNAPQAIADRAAF PGGLGDKVEDLTCCHPEREAELSHPSSDSEENE AKGRRATIAISSQEGDNSERTLSNNITVPKIERA NSYSAEEPSAPYAHTRKSFSISDKLDRQRNTAS LRNPFQRSKSSKPEGRGDSLSMRRLSRTSAFQS FESKHN*TFLISATEGSRIQP*NSLQLQFFPFP* IIPALFLAEQNKQCFGRC*LKGDFWATDQESI* SDPVPDTGDLRHVHTCWAGRGKRGRRKG*R*MC IRSHSRKP*EQGKQGASSTLHARRHLLISDHYQE L*DAGLNCKIK*NSQRTQ*DILNFHSVFFSHWL HHW*LMKSILFIQYKPAASSST*RPTSFSCQHF CN*SL*RKNKVTVIVHLSLIYSLLVPQLFILF*R K*GNRNAFLYCNRNERRVDVKKTKVK*FIIYSG RSSLVEQAQENVIK*FYIF*FILYLTCHG*IHS LNM*Y*T*KKKKK

Frame 2

LQQNESNRSCSGDCFCQYPESVLRLRNPG*KEH FIKENVSTSYPAPKTPIGVAVGV**ASMLASPP VSPCFRMRKMKVASPEMTSSLKSGPSANTLNSA LRMLLGPLSSKEVAIPTKPCMCEYLLIQNLISSY T**PRNGSWSFPSFSSLSMGACRTLNSSQNSSK SLGKGSSKQQ*QLERGYSLEGLTQVLFVMLAMP *RIMPLSLEERYAP*VLPPGELWKTRRTSLEEML SGHTRPCPIP*ASSLFSTACIPTSFWLTTGPLE NMEQR*NFEDNWKSIFHSRR*TQESVKVFLWWH S*WKEDPM*SRLFWSTFETPLPCQWLSVMGVDGH RTSWPLGINTQKKAD**MNL*GTSCW*LYRRLS HTLEPKLSICSSSSWSA*RRRN*LRYFGWDQKD TRTLIWLS*QLYSKEPMPRPQTN*A*L*PGTEST SLAARSLFTGNSGRWDLWSKPCWMP*FWTEWIL *NYS*RME*ACTVFSPSPD*RNCTIRDMGPQIH CTTWSGMSKRGTCPQTTESA*LTSAW*SST*WAG LIAATTRASASGPSTTTSSAPRGPKP*NCWEWR MIFP*GEEERQPRNVKKRWTLTWMILRSTTSPS LSMSSWCGLFS*SGRRWPCSSGSTVRRPWPRPWW PASSAKPWLMRPLRTTWLTTFPRS*ITIPETLA SWLWSSWTSPTSRTNSWP*NC*RMS*RTGATPR ACSLPWLPNTATSSRTRAARCCSPTCGWAGSACA RTQASR*FWEFYFLLQFSAWSSRTKTTCPICLR PRKSTSKRRRQKNQRSPQRKKRKRTWSSQQCWD ETTGSPPGRRMKRKFRASTG*SPSAEKSMNSTMH PS*SSGSTHWRISDT*CSSTISC**RWNAGRPP RNGS*SPIFSPWE*KR*ERF*CQSQGSCYRK*R YGCRSTGMSRTSSPSFCFLSE*SFVSKTSPSGVT GGSSTA*TSFTGISVS*TSSA*TSIWARM***L EK***T*CTLSSLCWWF**ALGSPGKPSFFPMR SHHGNWPRTSSTCPIG*FMGKCLRTR*TLPVDRM RPERMVK*SSCLPARQELGSCRPSWPATS*WQT SCWSTSSLLSLTIHFLK*NRYPTKSGSFRGISS S*LSMKGQFCPHH*SSSAT*P*YSSTCAADGGNT RATRMKGTTA*NSS*PMMSSRKYMTLKSNA*KN TSEKRMIGSTHLMMRGYG*LQKGWRTCLCGWRK STRESTP*RLHSRPWTSGWRSWKTLSGAWPRPWS A*QVWSGPSPTKSARGPRQTARTPPTLSVRAAS TARKGTPSSSKRV*TLQVRRPCPQLLQP*CPVC EAILSIRSI*KTKVV*KSWKVFLKKGP*AYTGLL VPTL*QKNPKLLQPLPTPWPLFLIPEDHHRV*T SMSLLWMSSTVI*TLWTIP*TSLGWASQAFQLQ YLPQPLQVVPMQHLHPQTDLQAGALILRTSPPWT LDLFLQTTPTSQNAKTPGTQSLRCTTPLSVPKV AAT*PPHPFF*KRLPL*NLIALCFPPQGAIMPT LGCL*KQQNTQVLQTVLTQGVSMPLKQLRTELPS LEVLETKWRT*LAAIQSEKQN*VTPALTVRRMR PKAAEPPLQYPPRRVITQREPCPTTSLFPR*SA PTATRQRSQVRHMHTPGRASPSVTNSTGSGTQQA CEIPSREASPPSRRAEGTACP*GDCPEHRLSKA LKASTTKPS*YPPQKAQESSPKILSNSSFSPFL ESYLLYS*LSKTSNALGGVNSKVTSGPQIKKAFD LTQCQTQGI*GMFTLAGQGGGREGEGRVRDECV SAVTAESHESRGNKGLRARSMPGGIC*FLTIIK SCRMQG*IAK*NKIASVHNEIF*TSILFSFHIGS ITGD**RASSLFSISRQQAVLPNVPHPSHANTS VIDHYKEKTR*QS*FTCLLSIHFWCHNCLSFFE ENKGTEMPFCIAIEMKEELMLKKQKSSDLLYTVG VQV*SSKLRRM*LNNFIFFNLFCISPVMDEFIH *ICNIELKKKKKK

Sequence NM_206944

AGCTGCAGCAAAATGAAAGCAACAGGAGCTGCTCCGGGGACTGCTTTTGCCAGTACCCAGAATCAGTGCTCAGGCTCAGAAATCCTGGATAGAAAGAGCA TTTTATAAAAGAGAATGTGTCCACATCATACCCAGCACCAAAGACCCCCATAGGTGTTGCTGTGGGCGTCTGATAGGCCAGCATGTTGGCCTCACCCCCA GTATCTCCGTGCTTCAGAATGAGAAAAATGAAAGTCGCCTCTCCCGAAATGACATCCAGTCTGAAAAGTGGTCCATCAGCAAACACACTCAACTCAGCCC TACGGATGCTTTTGGGACCATTGAGTTCCAAGGAGGTGGCCATTCCAACAAAGCCATGTATGTGCGAGTATCTTTTGATACAAAACCTGATCTCCTCTTA CACCTGATGACCAAGGAATGGCAGTTGGAGCTTCCCAAGCTTCTCATCTCTGTCCATGGGGGCCTGCAGAACTTTGAACTCCAGCCAAAACTCAAGCAAG TCTTTGGGAAAGGGCTCATCAAAGCAGCAATGACAACTGGAGCGTGGATATTCACTGGAGGGGTTAACACAGGTGTTATTCGTCATGTTGGCGATGCCTT GAAGGATCATGCCTCTAAGTCTCGAGGAAAGATATGCACCATAGGTATTGCCCCCTGGGGAATTGTGGAAAACCAGGAGGACCTCATTGGAAGAGATGTT GTCCGGCCATACCAGACCATGTCCAATCCCATGAGCAAGCTCACTGTTCTCAACAGCATGCATTCCCACTTCATTCTGGCTGACAACGGGACCACTGGAA AATATGGAGCAGAGGTGAAACTTCGAAGACAACTGGAAAAGCATATTTCACTCCAGAAGATAAACACAAGAATCGGTCAAGGTGTTCCTGTGGTGGCACT CATAGTGGAAGGAGGACCCAATGTGATCTCGATTGTTTTGGAGTACCTTCGAGACACCCCTCCCGTGCCAGTGGTTGTCTGTGATGGGAGTGGACGGGCA TCGGACATCCTGGCCTTTGGGCATAAATACTCAGAAGAAGGCGGACTGATAAATGAATCTTTGAGGGACCAGCTGTTGGTGACTATACAGAAGACTTTCA CATACACTCGAACCCAAGCTCAGCATCTGTTCATCATCCTCATGGAGTGCATGAAGAAGAAGGAATTGATTACGGTATTTCGGATGGGATCAGAAGGACA CCAGGACATTGATTTGGCTATCCTGACAGCTTTACTCAAAGGAGCCAATGCCTCGGCCCCAGACCAACTGAGCTTAGCTTTAGCCTGGAACAGAGTCGAC ATCGCTCGCAGCCAGATCTTTATTTACGGGCAACAGTGGCCGGTGGGATCTCTGGAGCAAGCCATGTTGGATGCCTTAGTTCTGGACAGAGTGGATTTTG TGAAATTACTCATAGAGAATGGAGTAAGCATGCACCGTTTTCTCACCATCTCCAGACTAGAGGAATTGTACAATACGAGACATGGGCCCTCAAATACATT GTACCACTTGGTCAGGGATGTCAAAAAGGGGAACCTGCCCCCAGACTACAGAATCAGCCTGATTGACATCGGCCTGGTGATCGAGTACCTGATGGGCGGG GCTTATCGCTGCAACTACACGCGCAAGCGCTTCCGGACCCTCTACCACAACCTCTTCGGCCCCAAGAGGGATGATATTCCCTTGAGGCGAGGAAGAAAGA CAACCAAGAAACGTGAAGAAGAGGTGGACATTGACTTGGATGATCCTGAGATCAACCACTTCCCCTTCCCTTTCCATGAGCTCATGGTGTGGGCTGTTCT CATGAAGCGGCAGAAGATGGCCCTGTTCTTCTGGCAGCACGGTGAGGAGGCCATGGCCAAGGCCCTGGTGGCCTGCAAGCTCTGCAAAGCCATGGCTCAT GAGGCCTCTGAGAACGACATGGTTGACGACATTTCCCAGGAGCTGAATCACAATTCCAGAGACTTTGGCCAGCTGGCTGTGGAGCTCCTGGACCAGTCCT ACAAGCAGGACGAACAGCTGGCCATGAAACTGCTGACGTATGAGCTGAAGAACTGGAGCAACGCCACGTGCCTGCAGCTTGCCGTGGCTGCCAAACACCG CGACTTCATCGCGCACACGTGCAGCCAGATGCTGCTCACCGACATGTGGATGGGCCGGCTCCGCATGCGCAAGAACTCAGGCCTCAAGGTAATTCTGGGA ATTCTACTTCCTCCTTCAATTCTCAGCTTGGAGTTCAAGAACAAAGACGACATGCCCTATATGTCTCAGGCCCAGGAAATCCACCTCCAAGAGAAGGAGG CAGAAGAACCAGAGAAGCCCACAAAGGAAAAAGAGGAAGAGGACATGGAGCTCACAGCAATGTTGGGACGAAACAACGGGGAGTCCTCCAGGAAGAAGGA TGAAGAGGAAGTTCAGAGCAAGCACCGGTTAATCCCCCTCGGCAGAAAAATCTATGAATTCTACAATGCACCCATCGTGAAGTTCTGGTTCTACACACTG GCGTATATCGGATACCTGATGCTCTTCAACTATATCGTGTTAGTGAAGATGGAACGCTGGCCGTCCACCCAGGAATGGATCGTAATCTCCTATATTTTCA CCCTGGGAATAGAAAAGATGAGAGAGATTCTGATGTCAGAGCCAGGGAAGTTGCTACAGAAAGTGAAGGTATGGCTGCAGGAGTACTGGAATGTCACGGA CCTCATCGCCATCCTTCTGTTTTCTGTCGGAATGATCCTTCGTCTCCAAGACCAGCCCTTCAGGAGTGACGGGAGGGTCATCTACTGCGTGAACATCATT TACTGGTATATCCGTCTCCTAGACATCTTCGGCGTGAACAAGTATTTGGGCCCGTATGTAATGATGATTGGAAAAATGATGATAGACATGATGTACTTTG TCATCATTATGCTGGTGGTTCTGATGAGCTTTGGGGTCGCCAGGCAAGCCATCCTTTTTCCCAATGAGGAGCCATCATGGAAACTGGCCAAGAACATCTT CTACATGCCCTATTGGATGATTTATGGGGAAGTGTTTGCGGACCAGATAGACCCTCCCTGTGGACAGAATGAGACCCGAGAGGATGGTAAAATAATCCAG CTGCCTCCCTGCAAGACAGGAGCTTGGATCGTGCCGGCCATCATGGCCTGCTACCTCTTAGTGGCAAACATCTTGCTGGTCAACCTCCTCATTGCTGTCT TTAACAATACATTTTTTGAAGTAAAATCGATATCCAACCAAGTCTGGAAGTTTCAGAGGTATCAGCTCATCATGACTTTCCATGAAAGGCCAGTTCTGCC CCCACCACTGATCATCTTCAGCCACATGACCATGATATTCCAGCACCTGTGCTGCCGATGGAGGAAACACGAGAGCGACCCGGATGAAAGGGACTACGGC CTGAAACTCTTCATAACCGATGATGAGCTCAAGAAAGTACATGACTTTGAAGAGCAATGCATAGAAGAATACTTCAGAGAAAAGGATGATCGGTTCAACT CATCTAATGATGAGAGGATACGGGTGACTTCAGAAAGGGTGGAGAACATGTCTATGCGGCTGGAGGAAGTCAACGAGAGAGAGCACTCCATGAAGGCTTC ACTCCAGACCGTGGACATCCGGCTGGCGCAGCTGGAAGACCTTATCGGGCGCATGGCCACGGCCCTGGAGCGCCTGACAGGTCTGGAGCGGGCCGAGTCC AACAAAATCCGCTCGAGGACCTCGTCAGACTGCACGGACGCCGCCTACATTGTCCGTCAGAGCAGCTTCAACAGCCAGGAAGGGAACACCTTCAAGCTCC AAGAGAGTATAGACCCTGCAGGTGAGGAGACCATGTCCCCAACTTCTCCAACCTTAATGCCCCGTATGCGAAGCCATTCTTTCTATTCGGTCAATATGAA AGACAAAGGTGGTATAGAAAAGTTGGAAAGTATTTTTAAAGAAAGGTCCCTGAGCCTACACCGGGCTACTAGTTCCCACTCTGTAGCAAAAGAACCCAAA GCTCCTGCAGCCCCTGCCAACACCTTGGCCATTGTTCCTGATTCCAGAAGACCATCATCGTGTATAGACATCTATGTCTCTGCTATGGATGAGCTCCACT GTGATATAGACCCTCTGGACAATTCCGTGAACATCCTTGGGCTGGGCGAGCCAAGCTTTTCAACTCCAGTACCTTCCACAGCCCCTTCAAGTAGTGCCTA TGCAACACTTGCACCCACAGACAGACCTCCAAGCCGGAGCATTGATTTTGAGGACATCACCTCCATGGACACTAGATCTTTTTCTTCAGACTACACCCAC CTCCCAGAATGCCAAAACCCCTGGGACTCAGAGCCTCCGATGTACCACACCATTGAGCGTTCCAAAAGTAGCCGCTACCTAGCCACCACACCCTTTCTTC TAGAAGAGGCTCCCATTGTGAAATCTCATAGCTTTATGTTTTCCCCCTCAAGGAGCTATTATGCCAACTTTGGGGTGCCTGTAAAAACAGCAGAATACAC AAGTATTACAGACTGTATTGACACAAGGTGTGTCAATGCCCCTCAAGCAATTGCGGACAGAGCTGCCTTCCCTGGAGGTCTTGGAGACAAAGTGGAGGAC TTAACTTGCTGCCATCCAGAGCGAGAAGCAGAACTGAGTCACCCCAGCTCTGACAGTGAGGAGAATGAGGCCAAAGGCCGCAGAGCCACCATTGCAATAT CCTCCCAGGAGGGTGATAACTCAGAGAGAACCCTGTCCAACAACATCACTGTTCCCAAGATAGAGCGCGCCAACAGCTACTCGGCAGAGGAGCCAAGTGC GCCATATGCACACACCAGGAAGAGCTTCTCCATCAGTGACAAACTCGACAGGCAGCGGAACACAGCAAGCCTGCGAAATCCCTTCCAGAGAAGCAAGTCC TCCAAGCCGGAGGGCCGAGGGGACAGCCTGTCCATGAGGAGACTGTCCAGAACATCGGCTTTCCAAAGCTTTGAAAGCAAGCACAACTAAACCTTCTTAA TATCCGCCACAGAAGGCTCAAGAATCCAGCCCTAAAATTCTCTCCAACTCCAGTTTTTCCCCTTTCCTTGAATCATACCTGCTTTATTCTTAGCTGAGCA AAACAAGCAATGCTTTGGGAGGTGTTAACTCAAAGGTGACTTCTGGGCCACAGATCAAGAAAGCATTTGATCTGACCCAGTGCCAGACACAGGGGATTTA AGGCATGTTCACACTTGCTGGGCAGGGAGGGGGAAGAGAGGGAGAAGGAAGGGTTAGAGATGAATGTGTATCCGCAGTCACAGCAGAAAGCCATGAGAGC AGGGGAAACAAGGGGCTTCGAGCACGCTCCATGCCAGGAGGCATCTGTTGATTTCTGACCATTATCAAGAGTTGTAGGATGCAGGGCTAAATTGCAAAAT AAAATAAAATAGCCAGCGTACACAATGAGATATTCTAAACTTCCATTCTGTTTTCTTTTCACATTGGCTCCATCACTGGTGACTGATGAAGAGCATCCTC TTTATTCAGTATAAGCCGGCAGCAAGCAGTTCTACCTAACGTCCCACATCCTTCTCATGCCAACACTTCTGTAATTGATCATTATAAAGAAAAAACAAGG TAACAGTCATAGTTCACCTGTCTCTTATCTATTCACTTCTGGTGCCACAACTGTTTATCCTTTTTTGAAGAAAATAAGGGAACAGAAATGCCTTTTTGTA TTGCAATCGAAATGAAAGAAGAGTTGATGTTAAAAAAACAAAAGTCAAGTGATTTATTATATACAGTGGGCGTTCAAGTCTAGTCGAGCAAGCTCAGGAG AATGTAATTAAATAATTTTATATTTTTTAATTTATTTTGTATCTCACCTGTCATGGATGAATTCATTCACTGAATATGTAATATTGAACTTAAAAAAAAA AAAAAAAAAA

refseq_TRPM3.F2 (forward primer)
refseq_TRPM3.R2 (reverse primer)
Single exon skipping

Frame 0

SCSKMKATGAAPGTAFASTQNQCSGSEILDRKS IL*KRMCPHHTQHQRPP*VLLWASDRPACWPHPQ YLRASE*EK*KSPLPK*HPV*KVVHQQTHSTQP YGCFWDH*VPRRWPFQQSHVCASIF*YKT*SPL TPDDQGMAVGASQASHLCPWGPAEL*TPAKTQAS LWERAHQSSNDNWSVDIHWRG*HRCYSSCWRCL EGSCL*VSRKDMHHRYCPLGNCGKPGGPHWKRC CPAIPDHVQSHEQAHCSQQHAFPLHSG*QRDHWK IWSRGETSKTTGKAYFTPEDKHKNRSRCSCGGT HSGRRTQCDLDCFGVPSRHPSRASGCL*WEWTG IGHPGLWA*ILRRRRTDK*IFEGPAVGDYTEDFH IHSNPSSASVHHPHGVHEEEGIDYGISDGIRRT PGH*FGYPDSFTQRSQCLGPRPTELSFSLEQSR HRSQPDLYLRATVAGGISGASHVGCLSSGQSGFC EITHREWSKHAPFSHHLQTRGIVQYETWALKYI VPLGQGCQKGEPAPRLQNQPD*HRPGDRVPDGR GLSLQLHAQALPDPLPQPLRPQEG*YSLEARKKD NQET*RRGGH*LG*S*DQPLPLPFP*AHGVGCS HEAAEDGPVLLAAR*GGHGQGPGGLQALQSHGS *GL*ERHG*RHFPGAESQFQRLWPAGCGAPGPVL QAGRTAGHETADV*AEELEQRHVPAACRGCQTP RLHRAHVQPDAAHRHVDGPAPHAQELRPQGNSG NSTSSFNSQLGVQEQRRHALYVSGPGNPPPREGG RRTREAHKGKRGRGHGAHSNVGTKQRGVLQEEG *RGSSEQAPVNPPRQKNL*ILQCTHREVLVLHT GVYRIPDALQLYRVSEDGTLAVHPGMDRNLLYFH PGNRKDERDSDVRAREVATESEGMAAGVLECHG PHRHPSVFCRNDPSSPRPALQE*REGHLLREHH LLVYPSPRHLRREQVFGPVCNDDWKNDDRHDVLC HHYAGGSDELWGRQASHPFSQ*GAIMETGQEHL LHALLDDLWGSVCGPDRPSLWTE*DPRGW*NNP AASLQDRSLDRAGHHGLLPLSGKHLAGQPPHCCL *QYIF*SKIDIQPSLEVSEVSAHHDFP*KASSA PTTDHLQPHDHDIPAPVLPMEETRERPG*KGLR PETLHNR**AQEST*L*RAMHRRILQRKG*SVQL I***EDTGDFRKGGEHVYAAGGSQRERALHEGF TPDRGHPAGAAGRPYRAHGHGPGAPDRSGAGRV QQNPLEDLVRLHGRRLHCPSEQLQQPGREHLQAP REYRPCR*GDHVPNFSNLNAPYAKPFFLFGQYE RQRWYRKVGKYF*RKVPEPTPGY*FPLCSKRTQ SSCSPCQHLGHCS*FQKTIIVYRHLCLCYG*APL *YRPSGQFREHPWAGRAKLFNSSTFHSPFK*CL CNTCTHRQTSKPEH*F*GHHLHGH*IFFFRLHP PPRMPKPLGLRASDVPHH*AFQK*PLPSHHTLSS RRGSHCEIS*LYVFPLKELLCQLWGACKNSRIH KYYRLY*HKVCQCPSSNCGQSCLPWRSWRQSGG LNLLPSRARSRTESPQL*Q*GE*GQRPQSHHCNI LPGG**LRENPVQQHHCSQDRARQQLLGRGAKC AICTHQEELLHQ*QTRQAAEHSKPAKSLPEKQV LQAGGPRGQPVHEETVQNIGFPKL*KQAQLNLLN IRHRRLKNPALKFSPTPVFPLSLNHTCFILS*A KQAMLWEVLTQR*LLGHRSRKHLI*PSARHRGF KACSHLLGREGEEREKEGLEMNVYPQSQQKAMRA GETRGFEHAPCQEASVDF*PLSRVVGCRAKLQN KIK*PAYTMRYSKLPFCFLFTLAPSLVTDEEHP LYSV*AGSKQFYLTSHILLMPTLL*LIIIKKKQG NSHSSPVSYLFTSGATTVYPFLKKIREQKCLFV LQSK*KKS*C*KNKSQVIYYIQWAFKSSRASSG ECN*IILYFLIYFVSHLSWMNSFTEYVILNLKKK KKK

Frame 1

AAAK*KQQELLRGLLLPVPRISAQAQKSWIERA FYKRECVHIIPSTKDPHRCCCGRLIGQHVGLTP SISVLQNEKNESRLSRNDIQSEKWSISKHTQLSP TDAFGTIEFQGGGHSNKAMYVRVSFDTKPDLLL HLMTKEWQLELPKLLISVHGGLQNFELQPKLKQ VFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDAL KDHASKSRGKICTIGIAPWGIVENQEDLIGRDV VRPYQTMSNPMSKLTVLNSMHSHFILADNGTTG KYGAEVKLRRQLEKHISLQKINTRIGQGVPVVAL IVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRA SDILAFGHKYSEEGGLINESLRDQLLVTIQKTF TYTRTQAQHLFIILMECMKKKELITVFRMGSEGH QDIDLAILTALLKGANASAPDQLSLALAWNRVD IARSQIFIYGQQWPVGSLEQAMLDALVLDRVDF VKLLIENGVSMHRFLTISRLEELYNTRHGPSNTL YHLVRDVKKGNLPPDYRISLIDIGLVIEYLMGG AYRCNYTRKRFRTLYHNLFGPKRDDIPLRRGRK TTKKREEEVDIDLDDPEINHFPFPFHELMVWAVL MKRQKMALFFWQHGEEAMAKALVACKLCKAMAH EASENDMVDDISQELNHNSRDFGQLAVELLDQS YKQDEQLAMKLLTYELKNWSNATCLQLAVAAKHR DFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILG ILLPPSILSLEFKNKDDMPYMSQAQEIHLQEKE AEEPEKPTKEKEEEDMELTAMLGRNNGESSRKKD EEEVQSKHRLIPLGRKIYEFYNAPIVKFWFYTL AYIGYLMLFNYIVLVKMERWPSTQEWIVISYIF TLGIEKMREILMSEPGKLLQKVKVWLQEYWNVTD LIAILLFSVGMILRLQDQPFRSDGRVIYCVNII YWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYF VIIMLVVLMSFGVARQAILFPNEEPSWKLAKNIF YMPYWMIYGEVFADQIDPPCGQNETREDGKIIQ LPPCKTGAWIVPAIMACYLLVANILLVNLLIAV FNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLP PPLIIFSHMTMIFQHLCCRWRKHESDPDERDYG LKLFITDDELKKVHDFEEQCIEEYFREKDDRFN SSNDERIRVTSERVENMSMRLEEVNEREHSMKAS LQTVDIRLAQLEDLIGRMATALERLTGLERAES NKIRSRTSSDCTDAAYIVRQSSFNSQEGNTFKL QESIDPAGEETMSPTSPTLMPRMRSHSFYSVNMK DKGGIEKLESIFKERSLSLHRATSSHSVAKEPK APAAPANTLAIVPDSRRPSSCIDIYVSAMDELH CDIDPLDNSVNILGLGEPSFSTPVPSTAPSSSAY ATLAPTDRPPSRSIDFEDITSMDTRSFSSDYTH LPECQNPWDSEPPMYHTIERSKSSRYLATTPFL LEEAPIVKSHSFMFSPSRSYYANFGVPVKTAEYT SITDCIDTRCVNAPQAIADRAAFPGGLGDKVED LTCCHPEREAELSHPSSDSEENEAKGRRATIAI SSQEGDNSERTLSNNITVPKIERANSYSAEEPSA PYAHTRKSFSISDKLDRQRNTASLRNPFQRSKS SKPEGRGDSLSMRRLSRTSAFQSFESKHN*TFL ISATEGSRIQP*NSLQLQFFPFP*IIPALFLAEQ NKQCFGRC*LKGDFWATDQESI*SDPVPDTGDL RHVHTCWAGRGKRGRRKG*R*MCIRSHSRKP*E QGKQGASSTLHARRHLLISDHYQEL*DAGLNCKI K*NSQRTQ*DILNFHSVFFSHWLHHW*LMKSIL FIQYKPAASSST*RPTSFSCQHFCN*SL*RKNK VTVIVHLSLIYSLLVPQLFILF*RK*GNRNAFLY CNRNERRVDVKKTKVK*FIIYSGRSSLVEQAQE NVIK*FYIF*FILYLTCHG*IHSLNM*Y*T*KK KKK

Frame 2

LQQNESNRSCSGDCFCQYPESVLRLRNPG*KEH FIKENVSTSYPAPKTPIGVAVGV**ASMLASPP VSPCFRMRKMKVASPEMTSSLKSGPSANTLNSA LRMLLGPLSSKEVAIPTKPCMCEYLLIQNLISSY T**PRNGSWSFPSFSSLSMGACRTLNSSQNSSK SLGKGSSKQQ*QLERGYSLEGLTQVLFVMLAMP *RIMPLSLEERYAP*VLPPGELWKTRRTSLEEML SGHTRPCPIP*ASSLFSTACIPTSFWLTTGPLE NMEQR*NFEDNWKSIFHSRR*TQESVKVFLWWH S*WKEDPM*SRLFWSTFETPLPCQWLSVMGVDGH RTSWPLGINTQKKAD**MNL*GTSCW*LYRRLS HTLEPKLSICSSSSWSA*RRRN*LRYFGWDQKD TRTLIWLS*QLYSKEPMPRPQTN*A*L*PGTEST SLAARSLFTGNSGRWDLWSKPCWMP*FWTEWIL *NYS*RME*ACTVFSPSPD*RNCTIRDMGPQIH CTTWSGMSKRGTCPQTTESA*LTSAW*SST*WAG LIAATTRASASGPSTTTSSAPRGMIFP*GEEER QPRNVKKRWTLTWMILRSTTSPSLSMSSWCGLF S*SGRRWPCSSGSTVRRPWPRPWWPASSAKPWLM RPLRTTWLTTFPRS*ITIPETLASWLWSSWTSP TSRTNSWP*NC*RMS*RTGATPRACSLPWLPNT ATSSRTRAARCCSPTCGWAGSACARTQASR*FWE FYFLLQFSAWSSRTKTTCPICLRPRKSTSKRRR QKNQRSPQRKKRKRTWSSQQCWDETTGSPPGRR MKRKFRASTG*SPSAEKSMNSTMHPS*SSGSTHW RISDT*CSSTISC**RWNAGRPPRNGS*SPIFS PWE*KR*ERF*CQSQGSCYRK*RYGCRSTGMSR TSSPSFCFLSE*SFVSKTSPSGVTGGSSTA*TSF TGISVS*TSSA*TSIWARM***LEK***T*CTL SSLCWWF**ALGSPGKPSFFPMRSHHGNWPRTS STCPIG*FMGKCLRTR*TLPVDRMRPERMVK*SS CLPARQELGSCRPSWPATS*WQTSCWSTSSLLS LTIHFLK*NRYPTKSGSFRGISSS*LSMKGQFC PHH*SSSAT*P*YSSTCAADGGNTRATRMKGTTA *NSS*PMMSSRKYMTLKSNA*KNTSEKRMIGST HLMMRGYG*LQKGWRTCLCGWRKSTRESTP*RL HSRPWTSGWRSWKTLSGAWPRPWSA*QVWSGPSP TKSARGPRQTARTPPTLSVRAASTARKGTPSSS KRV*TLQVRRPCPQLLQP*CPVCEAILSIRSI* KTKVV*KSWKVFLKKGP*AYTGLLVPTL*QKNPK LLQPLPTPWPLFLIPEDHHRV*TSMSLLWMSST VI*TLWTIP*TSLGWASQAFQLQYLPQPLQVVP MQHLHPQTDLQAGALILRTSPPWTLDLFLQTTPT SQNAKTPGTQSLRCTTPLSVPKVAAT*PPHPFF *KRLPL*NLIALCFPPQGAIMPTLGCL*KQQNT QVLQTVLTQGVSMPLKQLRTELPSLEVLETKWRT *LAAIQSEKQN*VTPALTVRRMRPKAAEPPLQY PPRRVITQREPCPTTSLFPR*SAPTATRQRSQV RHMHTPGRASPSVTNSTGSGTQQACEIPSREASP PSRRAEGTACP*GDCPEHRLSKALKASTTKPS* YPPQKAQESSPKILSNSSFSPFLESYLLYS*LS KTSNALGGVNSKVTSGPQIKKAFDLTQCQTQGI* GMFTLAGQGGGREGEGRVRDECVSAVTAESHES RGNKGLRARSMPGGIC*FLTIIKSCRMQG*IAK *NKIASVHNEIF*TSILFSFHIGSITGD**RASS LFSISRQQAVLPNVPHPSHANTSVIDHYKEKTR *QS*FTCLLSIHFWCHNCLSFFEENKGTEMPFC IAIEMKEELMLKKQKSSDLLYTVGVQV*SSKLRR M*LNNFIFFNLFCISPVMDEFIH*ICNIELKKK KKK

Sequence NM_206947

AGCTGCAGCAAAATGAAAGCAACAGGAGCTGCTCCGGGGACTGCTTTTGCCAGTACCCAGAATCAGTGCTCAGGCTCAGAAATCCTGGATAGAAAGAGCA TTTTATAAAAGAGAATGTGTCCACATCATACCCAGCACCAAAGACCCCCATAGGTGTTGCTGTGGGCGTCTGATAGGCCAGCATGTTGGCCTCACCCCCA GTATCTCCGTGCTTCAGAATGAGAAAAATGAAAGTCGCCTCTCCCGAAATGACATCCAGTCTGAAAAGTGGTCCATCAGCAAACACACTCAACTCAGCCC TACGGATGCTTTTGGGACCATTGAGTTCCAAGGAGGTGGCCATTCCAACAAAGCCATGTATGTGCGAGTATCTTTTGATACAAAACCTGATCTCCTCTTA CACCTGATGACCAAGGAATGGCAGTTGGAGCTTCCCAAGCTTCTCATCTCTGTCCATGGGGGCCTGCAGAACTTTGAACTCCAGCCAAAACTCAAGCAAG TCTTTGGGAAAGGGCTCATCAAAGCAGCAATGACAACTGGAGCGTGGATATTCACTGGAGGGGTTAACACAGGTGTTATTCGTCATGTTGGCGATGCCTT GAAGGATCATGCCTCTAAGTCTCGAGGAAAGATATGCACCATAGGTATTGCCCCCTGGGGAATTGTGGAAAACCAGGAGGACCTCATTGGAAGAGATGTT GTCCGGCCATACCAGACCATGTCCAATCCCATGAGCAAGCTCACTGTTCTCAACAGCATGCATTCCCACTTCATTCTGGCTGACAACGGGACCACTGGAA AATATGGAGCAGAGGTGAAACTTCGAAGACAACTGGAAAAGCATATTTCACTCCAGAAGATAAACACAAGATGCCTGCCGTTTTTCTCTCTTGACTCCCG CTTGTTTTATTCATTTTGGGGTAGTTGCCAGTTAGACTCAGTTGGAATCGGTCAAGGTGTTCCTGTGGTGGCACTCATAGTGGAAGGAGGACCCAATGTG ATCTCGATTGTTTTGGAGTACCTTCGAGACACCCCTCCCGTGCCAGTGGTTGTCTGTGATGGGAGTGGACGGGCATCGGACATCCTGGCCTTTGGGCATA AATACTCAGAAGAAGGCGGACTGATAAATGAATCTTTGAGGGACCAGCTGTTGGTGACTATACAGAAGACTTTCACATACACTCGAACCCAAGCTCAGCA TCTGTTCATCATCCTCATGGAGTGCATGAAGAAGAAGGAATTGATTACGGTATTTCGGATGGGATCAGAAGGACACCAGGACATTGATTTGGCTATCCTG ACAGCTTTACTCAAAGGAGCCAATGCCTCGGCCCCAGACCAACTGAGCTTAGCTTTAGCCTGGAACAGAGTCGACATCGCTCGCAGCCAGATCTTTATTT ACGGGCAACAGTGGCCGGTGGGATCTCTGGAGCAAGCCATGTTGGATGCCTTAGTTCTGGACAGAGTGGATTTTGTGAAATTACTCATAGAGAATGGAGT AAGCATGCACCGTTTTCTCACCATCTCCAGACTAGAGGAATTGTACAATACGAGACATGGGCCCTCAAATACATTGTACCACTTGGTCAGGGATGTCAAA AAGGGGAACCTGCCCCCAGACTACAGAATCAGCCTGATTGACATCGGCCTGGTGATCGAGTACCTGATGGGCGGGGCTTATCGCTGCAACTACACGCGCA AGCGCTTCCGGACCCTCTACCACAACCTCTTCGGCCCCAAGAGGGATGATATTCCCTTGAGGCGAGGAAGAAAGACAACCAAGAAACGTGAAGAAGAGGT GGACATTGACTTGGATGATCCTGAGATCAACCACTTCCCCTTCCCTTTCCATGAGCTCATGGTGTGGGCTGTTCTCATGAAGCGGCAGAAGATGGCCCTG TTCTTCTGGCAGCACGGTGAGGAGGCCATGGCCAAGGCCCTGGTGGCCTGCAAGCTCTGCAAAGCCATGGCTCATGAGGCCTCTGAGAACGACATGGTTG ACGACATTTCCCAGGAGCTGAATCACAATTCCAGAGACTTTGGCCAGCTGGCTGTGGAGCTCCTGGACCAGTCCTACAAGCAGGACGAACAGCTGGCCAT GAAACTGCTGACGTATGAGCTGAAGAACTGGAGCAACGCCACGTGCCTGCAGCTTGCCGTGGCTGCCAAACACCGCGACTTCATCGCGCACACGTGCAGC CAGATGCTGCTCACCGACATGTGGATGGGCCGGCTCCGCATGCGCAAGAACTCAGGCCTCAAGGTAATTCTGGGAATTCTACTTCCTCCTTCAATTCTCA GCTTGGAGTTCAAGAACAAAGACGACATGCCCTATATGTCTCAGGCCCAGGAAATCCACCTCCAAGAGAAGGAGGCAGAAGAACCAGAGAAGCCCACAAA GGAAAAAGAGGAAGAGGACATGGAGCTCACAGCAATGTTGGGACGAAACAACGGGGAGTCCTCCAGGAAGAAGGATGAAGAGGAAGTTCAGAGCAAGCAC CGGTTAATCCCCCTCGGCAGAAAAATCTATGAATTCTACAATGCACCCATCGTGAAGTTCTGGTTCTACACACTGGCGTATATCGGATACCTGATGCTCT TCAACTATATCGTGTTAGTGAAGATGGAACGCTGGCCGTCCACCCAGGAATGGATCGTAATCTCCTATATTTTCACCCTGGGAATAGAAAAGATGAGAGA GATTCTGATGTCAGAGCCAGGGAAGTTGCTACAGAAAGTGAAGGTATGGCTGCAGGAGTACTGGAATGTCACGGACCTCATCGCCATCCTTCTGTTTTCT GTCGGAATGATCCTTCGTCTCCAAGACCAGCCCTTCAGGAGTGACGGGAGGGTCATCTACTGCGTGAACATCATTTACTGGTATATCCGTCTCCTAGACA TCTTCGGCGTGAACAAGTATTTGGGCCCGTATGTAATGATGATTGGAAAAATGATGATAGACATGATGTACTTTGTCATCATTATGCTGGTGGTTCTGAT GAGCTTTGGGGTCGCCAGGCAAGCCATCCTTTTTCCCAATGAGGAGCCATCATGGAAACTGGCCAAGAACATCTTCTACATGCCCTATTGGATGATTTAT GGGGAAGTGTTTGCGGACCAGATAGACCCTCCCTGTGGACAGAATGAGACCCGAGAGGATGGTAAAATAATCCAGCTGCCTCCCTGCAAGACAGGAGCTT GGATCGTGCCGGCCATCATGGCCTGCTACCTCTTAGTGGCAAACATCTTGCTGGTCAACCTCCTCATTGCTGTCTTTAACAATACATTTTTTGAAGTAAA ATCGATATCCAACCAAGTCTGGAAGTTTCAGAGGTATCAGCTCATCATGACTTTCCATGAAAGGCCAGTTCTGCCCCCACCACTGATCATCTTCAGCCAC ATGACCATGATATTCCAGCACCTGTGCTGCCGATGGAGGAAACACGAGAGCGACCCGGATGAAAGGGACTACGGCCTGAAACTCTTCATAACCGATGATG AGCTCAAGAAAGTACATGACTTTGAAGAGCAATGCATAGAAGAATACTTCAGAGAAAAGGATGATCGGTTCAACTCATCTAATGATGAGAGGATACGGGT GACTTCAGAAAGGGTGGAGAACATGTCTATGCGGCTGGAGGAAGTCAACGAGAGAGAGCACTCCATGAAGGCTTCACTCCAGACCGTGGACATCCGGCTG GCGCAGCTGGAAGACCTTATCGGGCGCATGGCCACGGCCCTGGAGCGCCTGACAGGTCTGGAGCGGGCCGAGTCCAACAAAATCCGCTCGAGGACCTCGT CAGACTGCACGGACGCCGCCTACATTGTCCGTCAGAGCAGCTTCAACAGCCAGGAAGGGAACACCTTCAAGCTCCAAGAGAGTATAGACCCTGCAGGTGA GGAGACCATGTCCCCAACTTCTCCAACCTTAATGCCCCGTATGCGAAGCCATTCTTTCTATTCGGTCAATATGAAAGACAAAGGTGGTATAGAAAAGTTG GAAAGTATTTTTAAAGAAAGGTCCCTGAGCCTACACCGGGCTACTAGTTCCCACTCTGTAGCAAAAGAACCCAAAGCTCCTGCAGCCCCTGCCAACACCT TGGCCATTGTTCCTGATTCCAGAAGACCATCATCGTGTATAGACATCTATGTCTCTGCTATGGATGAGCTCCACTGTGATATAGACCCTCTGGACAATTC CGTGAACATCCTTGGGCTGGGCGAGCCAAGCTTTTCAACTCCAGTACCTTCCACAGCCCCTTCAAGTAGTGCCTATGCAACACTTGCACCCACAGACAGA CCTCCAAGCCGGAGCATTGATTTTGAGGACATCACCTCCATGGACACTAGATCTTTTTCTTCAGACTACACCCACCTCCCAGAATGCCAAAACCCCTGGG ACTCAGAGCCTCCGATGTACCACACCATTGAGCGTTCCAAAAGTAGCCGCTACCTAGCCACCACACCCTTTCTTCTAGAAGAGGCTCCCATTGTGAAATC TCATAGCTTTATGTTTTCCCCCTCAAGGAGCTATTATGCCAACTTTGGGGTGCCTGTAAAAACAGCAGAATACACAAGTATTACAGACTGTATTGACACA AGGTGTGTCAATGCCCCTCAAGCAATTGCGGACAGAGCTGCCTTCCCTGGAGGTCTTGGAGACAAAGTGGAGGACTTAACTTGCTGCCATCCAGAGCGAG AAGCAGAACTGAGTCACCCCAGCTCTGACAGTGAGGAGAATGAGGCCAAAGGCCGCAGAGCCACCATTGCAATATCCTCCCAGGAGGGTGATAACTCAGA GAGAACCCTGTCCAACAACATCACTGTTCCCAAGATAGAGCGCGCCAACAGCTACTCGGCAGAGGAGCCAAGTGCGCCATATGCACACACCAGGAAGAGC TTCTCCATCAGTGACAAACTCGACAGGCAGCGGAACACAGCAAGCCTGCGAAATCCCTTCCAGAGAAGCAAGTCCTCCAAGCCGGAGGGCCGAGGGGACA GCCTGTCCATGAGGAGACTGTCCAGAACATCGGCTTTCCAAAGCTTTGAAAGCAAGCACAACTAAACCTTCTTAATATCCGCCACAGAAGGCTCAAGAAT CCAGCCCTAAAATTCTCTCCAACTCCAGTTTTTCCCCTTTCCTTGAATCATACCTGCTTTATTCTTAGCTGAGCAAAACAAGCAATGCTTTGGGAGGTGT TAACTCAAAGGTGACTTCTGGGCCACAGATCAAGAAAGCATTTGATCTGACCCAGTGCCAGACACAGGGGATTTAAGGCATGTTCACACTTGCTGGGCAG GGAGGGGGAAGAGAGGGAGAAGGAAGGGTTAGAGATGAATGTGTATCCGCAGTCACAGCAGAAAGCCATGAGAGCAGGGGAAACAAGGGGCTTCGAGCAC GCTCCATGCCAGGAGGCATCTGTTGATTTCTGACCATTATCAAGAGTTGTAGGATGCAGGGCTAAATTGCAAAATAAAATAAAATAGCCAGCGTACACAA TGAGATATTCTAAACTTCCATTCTGTTTTCTTTTCACATTGGCTCCATCACTGGTGACTGATGAAGAGCATCCTCTTTATTCAGTATAAGCCGGCAGCAA GCAGTTCTACCTAACGTCCCACATCCTTCTCATGCCAACACTTCTGTAATTGATCATTATAAAGAAAAAACAAGGTAACAGTCATAGTTCACCTGTCTCT TATCTATTCACTTCTGGTGCCACAACTGTTTATCCTTTTTTGAAGAAAATAAGGGAACAGAAATGCCTTTTTGTATTGCAATCGAAATGAAAGAAGAGTT GATGTTAAAAAAACAAAAGTCAAGTGATTTATTATATACAGTGGGCGTTCAAGTCTAGTCGAGCAAGCTCAGGAGAATGTAATTAAATAATTTTATATTT TTTAATTTATTTTGTATCTCACCTGTCATGGATGAATTCATTCACTGAATATGTAATATTGAACTTAAAAAAAAAAAAAAAAAAA

refseq_TRPM3.F2 (forward primer)
refseq_TRPM3.R2 (reverse primer)
Single exon skipping

Frame 0

SCSKMKATGAAPGTAFASTQNQCSGSEILDRKS IL*KRMCPHHTQHQRPP*VLLWASDRPACWPHPQ YLRASE*EK*KSPLPK*HPV*KVVHQQTHSTQP YGCFWDH*VPRRWPFQQSHVCASIF*YKT*SPL TPDDQGMAVGASQASHLCPWGPAEL*TPAKTQAS LWERAHQSSNDNWSVDIHWRG*HRCYSSCWRCL EGSCL*VSRKDMHHRYCPLGNCGKPGGPHWKRC CPAIPDHVQSHEQAHCSQQHAFPLHSG*QRDHWK IWSRGETSKTTGKAYFTPEDKHKMPAVFLS*LP LVLFILG*LPVRLSWNRSRCSCGGTHSGRRTQC DLDCFGVPSRHPSRASGCL*WEWTGIGHPGLWA* ILRRRRTDK*IFEGPAVGDYTEDFHIHSNPSSA SVHHPHGVHEEEGIDYGISDGIRRTPGH*FGYP DSFTQRSQCLGPRPTELSFSLEQSRHRSQPDLYL RATVAGGISGASHVGCLSSGQSGFCEITHREWS KHAPFSHHLQTRGIVQYETWALKYIVPLGQGCQ KGEPAPRLQNQPD*HRPGDRVPDGRGLSLQLHAQ ALPDPLPQPLRPQEG*YSLEARKKDNQET*RRG GH*LG*S*DQPLPLPFP*AHGVGCSHEAAEDGP VLLAAR*GGHGQGPGGLQALQSHGS*GL*ERHG* RHFPGAESQFQRLWPAGCGAPGPVLQAGRTAGH ETADV*AEELEQRHVPAACRGCQTPRLHRAHVQ PDAAHRHVDGPAPHAQELRPQGNSGNSTSSFNSQ LGVQEQRRHALYVSGPGNPPPREGGRRTREAHK GKRGRGHGAHSNVGTKQRGVLQEEG*RGSSEQA PVNPPRQKNL*ILQCTHREVLVLHTGVYRIPDAL QLYRVSEDGTLAVHPGMDRNLLYFHPGNRKDER DSDVRAREVATESEGMAAGVLECHGPHRHPSVF CRNDPSSPRPALQE*REGHLLREHHLLVYPSPRH LRREQVFGPVCNDDWKNDDRHDVLCHHYAGGSD ELWGRQASHPFSQ*GAIMETGQEHLLHALLDDL WGSVCGPDRPSLWTE*DPRGW*NNPAASLQDRSL DRAGHHGLLPLSGKHLAGQPPHCCL*QYIF*SK IDIQPSLEVSEVSAHHDFP*KASSAPTTDHLQP HDHDIPAPVLPMEETRERPG*KGLRPETLHNR** AQEST*L*RAMHRRILQRKG*SVQLI***EDTG DFRKGGEHVYAAGGSQRERALHEGFTPDRGHPA GAAGRPYRAHGHGPGAPDRSGAGRVQQNPLEDLV RLHGRRLHCPSEQLQQPGREHLQAPREYRPCR* GDHVPNFSNLNAPYAKPFFLFGQYERQRWYRKV GKYF*RKVPEPTPGY*FPLCSKRTQSSCSPCQHL GHCS*FQKTIIVYRHLCLCYG*APL*YRPSGQF REHPWAGRAKLFNSSTFHSPFK*CLCNTCTHRQ TSKPEH*F*GHHLHGH*IFFFRLHPPPRMPKPLG LRASDVPHH*AFQK*PLPSHHTLSSRRGSHCEI S*LYVFPLKELLCQLWGACKNSRIHKYYRLY*H KVCQCPSSNCGQSCLPWRSWRQSGGLNLLPSRAR SRTESPQL*Q*GE*GQRPQSHHCNILPGG**LR ENPVQQHHCSQDRARQQLLGRGAKCAICTHQEE LLHQ*QTRQAAEHSKPAKSLPEKQVLQAGGPRGQ PVHEETVQNIGFPKL*KQAQLNLLNIRHRRLKN PALKFSPTPVFPLSLNHTCFILS*AKQAMLWEV LTQR*LLGHRSRKHLI*PSARHRGFKACSHLLGR EGEEREKEGLEMNVYPQSQQKAMRAGETRGFEH APCQEASVDF*PLSRVVGCRAKLQNKIK*PAYT MRYSKLPFCFLFTLAPSLVTDEEHPLYSV*AGSK QFYLTSHILLMPTLL*LIIIKKKQGNSHSSPVS YLFTSGATTVYPFLKKIREQKCLFVLQSK*KKS *C*KNKSQVIYYIQWAFKSSRASSGECN*IILYF LIYFVSHLSWMNSFTEYVILNLKKKKKK

Frame 1

AAAK*KQQELLRGLLLPVPRISAQAQKSWIERA FYKRECVHIIPSTKDPHRCCCGRLIGQHVGLTP SISVLQNEKNESRLSRNDIQSEKWSISKHTQLSP TDAFGTIEFQGGGHSNKAMYVRVSFDTKPDLLL HLMTKEWQLELPKLLISVHGGLQNFELQPKLKQ VFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDAL KDHASKSRGKICTIGIAPWGIVENQEDLIGRDV VRPYQTMSNPMSKLTVLNSMHSHFILADNGTTG KYGAEVKLRRQLEKHISLQKINTRCLPFFSLDSR LFYSFWGSCQLDSVGIGQGVPVVALIVEGGPNV ISIVLEYLRDTPPVPVVVCDGSGRASDILAFGH KYSEEGGLINESLRDQLLVTIQKTFTYTRTQAQH LFIILMECMKKKELITVFRMGSEGHQDIDLAIL TALLKGANASAPDQLSLALAWNRVDIARSQIFI YGQQWPVGSLEQAMLDALVLDRVDFVKLLIENGV SMHRFLTISRLEELYNTRHGPSNTLYHLVRDVK KGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTR KRFRTLYHNLFGPKRDDIPLRRGRKTTKKREEEV DIDLDDPEINHFPFPFHELMVWAVLMKRQKMAL FFWQHGEEAMAKALVACKLCKAMAHEASENDMV DDISQELNHNSRDFGQLAVELLDQSYKQDEQLAM KLLTYELKNWSNATCLQLAVAAKHRDFIAHTCS QMLLTDMWMGRLRMRKNSGLKVILGILLPPSIL SLEFKNKDDMPYMSQAQEIHLQEKEAEEPEKPTK EKEEEDMELTAMLGRNNGESSRKKDEEEVQSKH RLIPLGRKIYEFYNAPIVKFWFYTLAYIGYLML FNYIVLVKMERWPSTQEWIVISYIFTLGIEKMRE ILMSEPGKLLQKVKVWLQEYWNVTDLIAILLFS VGMILRLQDQPFRSDGRVIYCVNIIYWYIRLLD IFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLM SFGVARQAILFPNEEPSWKLAKNIFYMPYWMIY GEVFADQIDPPCGQNETREDGKIIQLPPCKTGA WIVPAIMACYLLVANILLVNLLIAVFNNTFFEVK SISNQVWKFQRYQLIMTFHERPVLPPPLIIFSH MTMIFQHLCCRWRKHESDPDERDYGLKLFITDD ELKKVHDFEEQCIEEYFREKDDRFNSSNDERIRV TSERVENMSMRLEEVNEREHSMKASLQTVDIRL AQLEDLIGRMATALERLTGLERAESNKIRSRTS SDCTDAAYIVRQSSFNSQEGNTFKLQESIDPAGE ETMSPTSPTLMPRMRSHSFYSVNMKDKGGIEKL ESIFKERSLSLHRATSSHSVAKEPKAPAAPANT LAIVPDSRRPSSCIDIYVSAMDELHCDIDPLDNS VNILGLGEPSFSTPVPSTAPSSSAYATLAPTDR PPSRSIDFEDITSMDTRSFSSDYTHLPECQNPW DSEPPMYHTIERSKSSRYLATTPFLLEEAPIVKS HSFMFSPSRSYYANFGVPVKTAEYTSITDCIDT RCVNAPQAIADRAAFPGGLGDKVEDLTCCHPER EAELSHPSSDSEENEAKGRRATIAISSQEGDNSE RTLSNNITVPKIERANSYSAEEPSAPYAHTRKS FSISDKLDRQRNTASLRNPFQRSKSSKPEGRGD SLSMRRLSRTSAFQSFESKHN*TFLISATEGSRI QP*NSLQLQFFPFP*IIPALFLAEQNKQCFGRC *LKGDFWATDQESI*SDPVPDTGDLRHVHTCWA GRGKRGRRKG*R*MCIRSHSRKP*EQGKQGASST LHARRHLLISDHYQEL*DAGLNCKIK*NSQRTQ *DILNFHSVFFSHWLHHW*LMKSILFIQYKPAA SSST*RPTSFSCQHFCN*SL*RKNKVTVIVHLSL IYSLLVPQLFILF*RK*GNRNAFLYCNRNERRV DVKKTKVK*FIIYSGRSSLVEQAQENVIK*FYI F*FILYLTCHG*IHSLNM*Y*T*KKKKK

Frame 2

LQQNESNRSCSGDCFCQYPESVLRLRNPG*KEH FIKENVSTSYPAPKTPIGVAVGV**ASMLASPP VSPCFRMRKMKVASPEMTSSLKSGPSANTLNSA LRMLLGPLSSKEVAIPTKPCMCEYLLIQNLISSY T**PRNGSWSFPSFSSLSMGACRTLNSSQNSSK SLGKGSSKQQ*QLERGYSLEGLTQVLFVMLAMP *RIMPLSLEERYAP*VLPPGELWKTRRTSLEEML SGHTRPCPIP*ASSLFSTACIPTSFWLTTGPLE NMEQR*NFEDNWKSIFHSRR*TQDACRFSLLTP ACFIHFGVVAS*TQLESVKVFLWWHS*WKEDPM* SRLFWSTFETPLPCQWLSVMGVDGHRTSWPLGI NTQKKAD**MNL*GTSCW*LYRRLSHTLEPKLS ICSSSSWSA*RRRN*LRYFGWDQKDTRTLIWLS* QLYSKEPMPRPQTN*A*L*PGTESTSLAARSLF TGNSGRWDLWSKPCWMP*FWTEWIL*NYS*RME *ACTVFSPSPD*RNCTIRDMGPQIHCTTWSGMSK RGTCPQTTESA*LTSAW*SST*WAGLIAATTRA SASGPSTTTSSAPRGMIFP*GEEERQPRNVKKR WTLTWMILRSTTSPSLSMSSWCGLFS*SGRRWPC SSGSTVRRPWPRPWWPASSAKPWLMRPLRTTWL TTFPRS*ITIPETLASWLWSSWTSPTSRTNSWP *NC*RMS*RTGATPRACSLPWLPNTATSSRTRAA RCCSPTCGWAGSACARTQASR*FWEFYFLLQFS AWSSRTKTTCPICLRPRKSTSKRRRQKNQRSPQ RKKRKRTWSSQQCWDETTGSPPGRRMKRKFRAST G*SPSAEKSMNSTMHPS*SSGSTHWRISDT*CS STISC**RWNAGRPPRNGS*SPIFSPWE*KR*E RF*CQSQGSCYRK*RYGCRSTGMSRTSSPSFCFL SE*SFVSKTSPSGVTGGSSTA*TSFTGISVS*T SSA*TSIWARM***LEK***T*CTLSSLCWWF* *ALGSPGKPSFFPMRSHHGNWPRTSSTCPIG*FM GKCLRTR*TLPVDRMRPERMVK*SSCLPARQEL GSCRPSWPATS*WQTSCWSTSSLLSLTIHFLK* NRYPTKSGSFRGISSS*LSMKGQFCPHH*SSSAT *P*YSSTCAADGGNTRATRMKGTTA*NSS*PMM SSRKYMTLKSNA*KNTSEKRMIGSTHLMMRGYG *LQKGWRTCLCGWRKSTRESTP*RLHSRPWTSGW RSWKTLSGAWPRPWSA*QVWSGPSPTKSARGPR QTARTPPTLSVRAASTARKGTPSSSKRV*TLQV RRPCPQLLQP*CPVCEAILSIRSI*KTKVV*KSW KVFLKKGP*AYTGLLVPTL*QKNPKLLQPLPTP WPLFLIPEDHHRV*TSMSLLWMSSTVI*TLWTI P*TSLGWASQAFQLQYLPQPLQVVPMQHLHPQTD LQAGALILRTSPPWTLDLFLQTTPTSQNAKTPG TQSLRCTTPLSVPKVAAT*PPHPFF*KRLPL*N LIALCFPPQGAIMPTLGCL*KQQNTQVLQTVLTQ GVSMPLKQLRTELPSLEVLETKWRT*LAAIQSE KQN*VTPALTVRRMRPKAAEPPLQYPPRRVITQ REPCPTTSLFPR*SAPTATRQRSQVRHMHTPGRA SPSVTNSTGSGTQQACEIPSREASPPSRRAEGT ACP*GDCPEHRLSKALKASTTKPS*YPPQKAQE SSPKILSNSSFSPFLESYLLYS*LSKTSNALGGV NSKVTSGPQIKKAFDLTQCQTQGI*GMFTLAGQ GGGREGEGRVRDECVSAVTAESHESRGNKGLRA RSMPGGIC*FLTIIKSCRMQG*IAK*NKIASVHN EIF*TSILFSFHIGSITGD**RASSLFSISRQQ AVLPNVPHPSHANTSVIDHYKEKTR*QS*FTCL LSIHFWCHNCLSFFEENKGTEMPFCIAIEMKEEL MLKKQKSSDLLYTVGVQV*SSKLRRM*LNNFIF FNLFCISPVMDEFIH*ICNIELKKKKKK

Sequence NM_024971

AGCTGCAGCAAAATGAAAGCAACAGGAGCTGCTCCGGGGACTGCTTTTGCCAGTACCCAGAATCAGTGCTCAGGCTCAGAAATCCTGGATAGAAAGAGCA TTTTATAAAAGAGAATGTGTCCACATCATACCCAGCACCAAAGACCCCCATAGGTGTTGCTGTGGGCGTCTGATAGGCCAGCATGTTGGCCTCACCCCCA GTATCTCCGTGCTTCAGAATGAGAAAAATGAAAGTCGCCTCTCCCGAAATGACATCCAGTCTGAAAAGTGGTCCATCAGCAAACACACTCAACTCAGCCC TACGGATGCTTTTGGGACCATTGAGTTCCAAGGAGGTGGCCATTCCAACAAAGCCATGTATGTGCGAGTATCTTTTGATACAAAACCTGATCTCCTCTTA CACCTGATGACCAAGGAATGGCAGTTGGAGCTTCCCAAGCTTCTCATCTCTGTCCATGGGGGCCTGCAGAACTTTGAACTCCAGCCAAAACTCAAGCAAG TCTTTGGGAAAGGGCTCATCAAAGCAGCAATGACAACTGGAGCGTGGATATTCACTGGAGGGGTTAACACAGGTGTTATTCGTCATGTTGGCGATGCCTT GAAGGATCATGCCTCTAAGTCTCGAGGAAAGATATGCACCATAGGTATTGCCCCCTGGGGAATTGTGGAAAACCAGGAGGACCTCATTGGAAGAGATGTT GTCCGGCCATACCAGACCATGTCCAATCCCATGAGCAAGCTCACTGTTCTCAACAGCATGCATTCCCACTTCATTCTGGCTGACAACGGGACCACTGGAA AATATGGAGCAGAGGTGAAACTTCGAAGACAACTGGAAAAGCATATTTCACTCCAGAAGATAAACACAAGAATCGGTCAAGGTGTTCCTGTGGTGGCACT CATAGTGGAAGGAGGACCCAATGTGATCTCGATTGTTTTGGAGTACCTTCGAGACACCCCTCCCGTGCCAGTGGTTGTCTGTGATGGGAGTGGACGGGCA TCGGACATCCTGGCCTTTGGGCATAAATACTCAGAAGAAGGCGGACTGATAAATGAATCTTTGAGGGACCAGCTGTTGGTGACTATACAGAAGACTTTCA CATACACTCGAACCCAAGCTCAGCATCTGTTCATCATCCTCATGGAGTGCATGAAGAAGAAGGAATTGATTACGGTATTTCGGATGGGATCAGAAGGACA CCAGGACATTGATTTGGCTATCCTGACAGCTTTACTCAAAGGAGCCAATGCCTCGGCCCCAGACCAACTGAGCTTAGCTTTAGCCTGGAACAGAGTCGAC ATCGCTCGCAGCCAGATCTTTATTTACGGGCAACAGTGGCCGGTGGGATCTCTGGAGCAAGCCATGTTGGATGCCTTAGTTCTGGACAGAGTGGATTTTG TGAAATTACTCATAGAGAATGGAGTAAGCATGCACCGTTTTCTCACCATCTCCAGACTAGAGGAATTGTACAATACGAGACATGGGCCCTCAAATACATT GTACCACTTGGTCAGGGATGTCAAAAAGCGAGAGTATCCAGGTTTCGGTTGGATCTATTTTAAGGGGAACCTGCCCCCAGACTACAGAATCAGCCTGATT GACATCGGCCTGGTGATCGAGTACCTGATGGGCGGGGCTTATCGCTGCAACTACACGCGCAAGCGCTTCCGGACCCTCTACCACAACCTCTTCGGCCCCA AGAGGCCCAAAGCCTTGAAACTGCTGGGAATGGAGGATGATATTCCCTTGAGGCGAGGAAGAAAGACAACCAAGAAACGTGAAGAAGAGGTGGACATTGA CTTGGATGATCCTGAGATCAACCACTTCCCCTTCCCTTTCCATGAGCTCATGGTGTGGGCTGTTCTCATGAAGCGGCAGAAGATGGCCCTGTTCTTCTGG CAGCACGGTGAGGAGGCCATGGCCAAGGCCCTGGTGGCCTGCAAGCTCTGCAAAGCCATGGCTCATGAGGCCTCTGAGAACGACATGGTTGACGACATTT CCCAGGAGCTGAATCACAATTCCAGAGACTTTGGCCAGCTGGCTGTGGAGCTCCTGGACCAGTCCTACAAGCAGGACGAACAGCTGGCCATGAAACTGCT GACGTATGAGCTGAAGAACTGGAGCAACGCCACGTGCCTGCAGCTTGCCGTGGCTGCCAAACACCGCGACTTCATCGCGCACACGTGCAGCCAGATGCTG CTCACCGACATGTGGATGGGCCGGCTCCGCATGCGCAAGAACTCAGGCCTCAAGGTAATTCTGGGAATTCTACTTCCTCCTTCAATTCTCAGCTTGGAGT TCAAGAACAAAGACGACATGCCCTATATGTCTCAGGCCCAGGAAATCCACCTCCAAGAGAAGGAGGCAGAAGAACCAGAGAAGCCCACAAAGGAAAAAGA GGAAGAGGACATGGAGCTCACAGCAATGTTGGGACGAAACAACGGGGAGTCCTCCAGGAAGAAGGATGAAGAGGAAGTTCAGAGCAAGCACCGGTTAATC CCCCTCGGCAGAAAAATCTATGAATTCTACAATGCACCCATCGTGAAGTTCTGGTTCTACACACTGGCGTATATCGGATACCTGATGCTCTTCAACTATA TCGTGTTAGTGAAGATGGAACGCTGGCCGTCCACCCAGGAATGGATCGTAATCTCCTATATTTTCACCCTGGGAATAGAAAAGATGAGAGAGATTCTGAT GTCAGAGCCAGGGAAGTTGCTACAGAAAGTGAAGGTATGGCTGCAGGAGTACTGGAATGTCACGGACCTCATCGCCATCCTTCTGTTTTCTGTCGGAATG ATCCTTCGTCTCCAAGACCAGCCCTTCAGGAGTGACGGGAGGGTCATCTACTGCGTGAACATCATTTACTGGTATATCCGTCTCCTAGACATCTTCGGCG TGAACAAGTATTTGGGCCCGTATGTAATGATGATTGGAAAAATGATGATAGACATGATGTACTTTGTCATCATTATGCTGGTGGTTCTGATGAGCTTTGG GGTCGCCAGGCAAGCCATCCTTTTTCCCAATGAGGAGCCATCATGGAAACTGGCCAAGAACATCTTCTACATGCCCTATTGGATGATTTATGGGGAAGTG TTTGCGGACCAGATAGACCCTCCCTGTGGACAGAATGAGACCCGAGAGGATGGTAAAATAATCCAGCTGCCTCCCTGCAAGACAGGAGCTTGGATCGTGC CGGCCATCATGGCCTGCTACCTCTTAGTGGCAAACATCTTGCTGGTCAACCTCCTCATTGCTGTCTTTAACAATACATTTTTTGAAGTAAAATCGATATC CAACCAAGTCTGGAAGTTTCAGAGGTATCAGCTCATCATGACTTTCCATGAAAGGCCAGTTCTGCCCCCACCACTGATCATCTTCAGCCACATGACCATG ATATTCCAGCACCTGTGCTGCCGATGGAGGAAACACGAGAGCGACCCGGATGAAAGGGACTACGGCCTGAAACTCTTCATAACCGATGATGAGCTCAAGA AAGTACATGACTTTGAAGAGCAATGCATAGAAGAATACTTCAGAGAAAAGGATGATCGGTTCAACTCATCTAATGATGAGAGGATACGGGTGACTTCAGA AAGGGTGGAGAACATGTCTATGCGGCTGGAGGAAGTCAACGAGAGAGAGCACTCCATGAAGGCTTCACTCCAGACCGTGGACATCCGGCTGGCGCAGCTG GAAGACCTTATCGGGCGCATGGCCACGGCCCTGGAGCGCCTGACAGGTCTGGAGCGGGCCGAGTCCAACAAAATCCGCTCGAGGACCTCGTCAGACTGCA CGGACGCCGCCTACATTGTCCGTCAGAGCAGCTTCAACAGCCAGGAAGGGAACACCTTCAAGCTCCAAGAGAGTATAGACCCTGCAGGTGAGGAGACCAT GTCCCCAACTTCTCCAACCTTAATGCCCCGTATGCGAAGCCATTCTTTCTATTCGGTCAATATGAAAGACAAAGGTGGTATAGAAAAGTTGGAAAGTATT TTTAAAGAAAGGTCCCTGAGCCTACACCGGGCTACTAGTTCCCACTCTGTAGCAAAAGAACCCAAAGCTCCTGCAGCCCCTGCCAACACCTTGGCCATTG TTCCTGATTCCAGAAGACCATCATCGTGTATAGACATCTATGTCTCTGCTATGGATGAGCTCCACTGTGATATAGACCCTCTGGACAATTCCGTGAACAT CCTTGGGCTGGGCGAGCCAAGCTTTTCAACTCCAGTACCTTCCACAGCCCCTTCAAGTAGTGCCTATGCAACACTTGCACCCACAGACAGACCTCCAAGC CGGAGCATTGATTTTGAGGACATCACCTCCATGGACACTAGATCTTTTTCTTCAGACTACACCCACCTCCCAGAATGCCAAAACCCCTGGGACTCAGAGC CTCCGATGTACCACACCATTGAGCGTTCCAAAAGTAGCCGCTACCTAGCCACCACACCCTTTCTTCTAGAAGAGGCTCCCATTGTGAAATCTCATAGCTT TATGTTTTCCCCCTCAAGGAGCTATTATGCCAACTTTGGGGTGCCTGTAAAAACAGCAGAATACACAAGTATTACAGACTGTATTGACACAAGGTGTGTC AATGCCCCTCAAGCAATTGCGGACAGAGCTGCCTTCCCTGGAGGTCTTGGAGACAAAGTGGAGGACTTAACTTGCTGCCATCCAGAGCGAGAAGCAGAAC TGAGTCACCCCAGCTCTGACAGTGAGGAGAATGAGGCCAAAGGCCGCAGAGCCACCATTGCAATATCCTCCCAGGAGGGTGATAACTCAGAGAGAACCCT GTCCAACAACATCACTGTTCCCAAGATAGAGCGCGCCAACAGCTACTCGGCAGAGGAGCCAAGTGCGCCATATGCACACACCAGGAAGAGCTTCTCCATC AGTGACAAACTCGACAGGCAGCGGAACACAGCAAGCCTGCGAAATCCCTTCCAGAGAAGCAAGTCCTCCAAGCCGGAGGGCCGAGGGGACAGCCTGTCCA TGAGGAGACTGTCCAGAACATCGGCTTTCCAAAGCTTTGAAAGCAAGCACAACTAAACCTTCTTAATATCCGCCACAGAAGGCTCAAGAATCCAGCCCTA AAATTCTCTCCAACTCCAGTTTTTCCCCTTTCCTTGAATCATACCTGCTTTATTCTTAGCTGAGCAAAACAAGCAATGCTTTGGGAGGTGTTAACTCAAA GGTGACTTCTGGGCCACAGATCAAGAAAGCATTTGATCTGACCCAGTGCCAGACACAGGGGATTTAAGGCATGTTCACACTTGCTGGGCAGGGAGGGGGA AGAGAGGGAGAAGGAAGGGTTAGAGATGAATGTGTATCCGCAGTCACAGCAGAAAGCCATGAGAGCAGGGGAAACAAGGGGCTTCGAGCACGCTCCATGC CAGGAGGCATCTGTTGATTTCTGACCATTATCAAGAGTTGTAGGATGCAGGGCTAAATTGCAAAATAAAATAAAATAGCCAGCGTACACAATGAGATATT CTAAACTTCCATTCTGTTTTCTTTTCACATTGGCTCCATCACTGGTGACTGATGAAGAGCATCCTCTTTATTCAGTATAAGCCGGCAGCAAGCAGTTCTA CCTAACGTCCCACATCCTTCTCATGCCAACACTTCTGTAATTGATCATTATAAAGAAAAAACAAGGTAACAGTCATAGTTCACCTGTCTCTTATCTATTC ACTTCTGGTGCCACAACTGTTTATCCTTTTTTGAAGAAAATAAGGGAACAGAAATGCCTTTTTGTATTGCAATCGAAATGAAAGAAGAGTTGATGTTAAA AAAACAAAAGTCAAGTGATTTATTATATACAGTGGGCGTTCAAGTCTAGTCGAGCAAGCTCAGGAGAATGTAATTAAATAATTTTATATTTTTTAATTTA TTTTGTATCTCACCTGTCATGGATGAATTCATTCACTGAATATGTAATATTGAACTTAAAAAAAAAAAAAAAAAAA

refseq_TRPM3.F2 (forward primer)
refseq_TRPM3.R2 (reverse primer)
Single exon skipping

Frame 0

SCSKMKATGAAPGTAFASTQNQCSGSEILDRKS IL*KRMCPHHTQHQRPP*VLLWASDRPACWPHPQ YLRASE*EK*KSPLPK*HPV*KVVHQQTHSTQP YGCFWDH*VPRRWPFQQSHVCASIF*YKT*SPL TPDDQGMAVGASQASHLCPWGPAEL*TPAKTQAS LWERAHQSSNDNWSVDIHWRG*HRCYSSCWRCL EGSCL*VSRKDMHHRYCPLGNCGKPGGPHWKRC CPAIPDHVQSHEQAHCSQQHAFPLHSG*QRDHWK IWSRGETSKTTGKAYFTPEDKHKNRSRCSCGGT HSGRRTQCDLDCFGVPSRHPSRASGCL*WEWTG IGHPGLWA*ILRRRRTDK*IFEGPAVGDYTEDFH IHSNPSSASVHHPHGVHEEEGIDYGISDGIRRT PGH*FGYPDSFTQRSQCLGPRPTELSFSLEQSR HRSQPDLYLRATVAGGISGASHVGCLSSGQSGFC EITHREWSKHAPFSHHLQTRGIVQYETWALKYI VPLGQGCQKARVSRFRLDLF*GEPAPRLQNQPD *HRPGDRVPDGRGLSLQLHAQALPDPLPQPLRPQ EAQSLETAGNGG*YSLEARKKDNQET*RRGGH* LG*S*DQPLPLPFP*AHGVGCSHEAAEDGPVLL AAR*GGHGQGPGGLQALQSHGS*GL*ERHG*RHF PGAESQFQRLWPAGCGAPGPVLQAGRTAGHETA DV*AEELEQRHVPAACRGCQTPRLHRAHVQPDA AHRHVDGPAPHAQELRPQGNSGNSTSSFNSQLGV QEQRRHALYVSGPGNPPPREGGRRTREAHKGKR GRGHGAHSNVGTKQRGVLQEEG*RGSSEQAPVN PPRQKNL*ILQCTHREVLVLHTGVYRIPDALQLY RVSEDGTLAVHPGMDRNLLYFHPGNRKDERDSD VRAREVATESEGMAAGVLECHGPHRHPSVFCRN DPSSPRPALQE*REGHLLREHHLLVYPSPRHLRR EQVFGPVCNDDWKNDDRHDVLCHHYAGGSDELW GRQASHPFSQ*GAIMETGQEHLLHALLDDLWGS VCGPDRPSLWTE*DPRGW*NNPAASLQDRSLDRA GHHGLLPLSGKHLAGQPPHCCL*QYIF*SKIDI QPSLEVSEVSAHHDFP*KASSAPTTDHLQPHDH DIPAPVLPMEETRERPG*KGLRPETLHNR**AQE ST*L*RAMHRRILQRKG*SVQLI***EDTGDFR KGGEHVYAAGGSQRERALHEGFTPDRGHPAGAA GRPYRAHGHGPGAPDRSGAGRVQQNPLEDLVRLH GRRLHCPSEQLQQPGREHLQAPREYRPCR*GDH VPNFSNLNAPYAKPFFLFGQYERQRWYRKVGKY F*RKVPEPTPGY*FPLCSKRTQSSCSPCQHLGHC S*FQKTIIVYRHLCLCYG*APL*YRPSGQFREH PWAGRAKLFNSSTFHSPFK*CLCNTCTHRQTSK PEH*F*GHHLHGH*IFFFRLHPPPRMPKPLGLRA SDVPHH*AFQK*PLPSHHTLSSRRGSHCEIS*L YVFPLKELLCQLWGACKNSRIHKYYRLY*HKVC QCPSSNCGQSCLPWRSWRQSGGLNLLPSRARSRT ESPQL*Q*GE*GQRPQSHHCNILPGG**LRENP VQQHHCSQDRARQQLLGRGAKCAICTHQEELLH Q*QTRQAAEHSKPAKSLPEKQVLQAGGPRGQPVH EETVQNIGFPKL*KQAQLNLLNIRHRRLKNPAL KFSPTPVFPLSLNHTCFILS*AKQAMLWEVLTQ R*LLGHRSRKHLI*PSARHRGFKACSHLLGREGE EREKEGLEMNVYPQSQQKAMRAGETRGFEHAPC QEASVDF*PLSRVVGCRAKLQNKIK*PAYTMRY SKLPFCFLFTLAPSLVTDEEHPLYSV*AGSKQFY LTSHILLMPTLL*LIIIKKKQGNSHSSPVSYLF TSGATTVYPFLKKIREQKCLFVLQSK*KKS*C* KNKSQVIYYIQWAFKSSRASSGECN*IILYFLIY FVSHLSWMNSFTEYVILNLKKKKKK

Frame 1

AAAK*KQQELLRGLLLPVPRISAQAQKSWIERA FYKRECVHIIPSTKDPHRCCCGRLIGQHVGLTP SISVLQNEKNESRLSRNDIQSEKWSISKHTQLSP TDAFGTIEFQGGGHSNKAMYVRVSFDTKPDLLL HLMTKEWQLELPKLLISVHGGLQNFELQPKLKQ VFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDAL KDHASKSRGKICTIGIAPWGIVENQEDLIGRDV VRPYQTMSNPMSKLTVLNSMHSHFILADNGTTG KYGAEVKLRRQLEKHISLQKINTRIGQGVPVVAL IVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRA SDILAFGHKYSEEGGLINESLRDQLLVTIQKTF TYTRTQAQHLFIILMECMKKKELITVFRMGSEGH QDIDLAILTALLKGANASAPDQLSLALAWNRVD IARSQIFIYGQQWPVGSLEQAMLDALVLDRVDF VKLLIENGVSMHRFLTISRLEELYNTRHGPSNTL YHLVRDVKKREYPGFGWIYFKGNLPPDYRISLI DIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGP KRPKALKLLGMEDDIPLRRGRKTTKKREEEVDID LDDPEINHFPFPFHELMVWAVLMKRQKMALFFW QHGEEAMAKALVACKLCKAMAHEASENDMVDDI SQELNHNSRDFGQLAVELLDQSYKQDEQLAMKLL TYELKNWSNATCLQLAVAAKHRDFIAHTCSQML LTDMWMGRLRMRKNSGLKVILGILLPPSILSLE FKNKDDMPYMSQAQEIHLQEKEAEEPEKPTKEKE EEDMELTAMLGRNNGESSRKKDEEEVQSKHRLI PLGRKIYEFYNAPIVKFWFYTLAYIGYLMLFNY IVLVKMERWPSTQEWIVISYIFTLGIEKMREILM SEPGKLLQKVKVWLQEYWNVTDLIAILLFSVGM ILRLQDQPFRSDGRVIYCVNIIYWYIRLLDIFG VNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFG VARQAILFPNEEPSWKLAKNIFYMPYWMIYGEV FADQIDPPCGQNETREDGKIIQLPPCKTGAWIV PAIMACYLLVANILLVNLLIAVFNNTFFEVKSIS NQVWKFQRYQLIMTFHERPVLPPPLIIFSHMTM IFQHLCCRWRKHESDPDERDYGLKLFITDDELK KVHDFEEQCIEEYFREKDDRFNSSNDERIRVTSE RVENMSMRLEEVNEREHSMKASLQTVDIRLAQL EDLIGRMATALERLTGLERAESNKIRSRTSSDC TDAAYIVRQSSFNSQEGNTFKLQESIDPAGEETM SPTSPTLMPRMRSHSFYSVNMKDKGGIEKLESI FKERSLSLHRATSSHSVAKEPKAPAAPANTLAI VPDSRRPSSCIDIYVSAMDELHCDIDPLDNSVNI LGLGEPSFSTPVPSTAPSSSAYATLAPTDRPPS RSIDFEDITSMDTRSFSSDYTHLPECQNPWDSE PPMYHTIERSKSSRYLATTPFLLEEAPIVKSHSF MFSPSRSYYANFGVPVKTAEYTSITDCIDTRCV NAPQAIADRAAFPGGLGDKVEDLTCCHPEREAE LSHPSSDSEENEAKGRRATIAISSQEGDNSERTL SNNITVPKIERANSYSAEEPSAPYAHTRKSFSI SDKLDRQRNTASLRNPFQRSKSSKPEGRGDSLS MRRLSRTSAFQSFESKHN*TFLISATEGSRIQP* NSLQLQFFPFP*IIPALFLAEQNKQCFGRC*LK GDFWATDQESI*SDPVPDTGDLRHVHTCWAGRG KRGRRKG*R*MCIRSHSRKP*EQGKQGASSTLHA RRHLLISDHYQEL*DAGLNCKIK*NSQRTQ*DI LNFHSVFFSHWLHHW*LMKSILFIQYKPAASSS T*RPTSFSCQHFCN*SL*RKNKVTVIVHLSLIYS LLVPQLFILF*RK*GNRNAFLYCNRNERRVDVK KTKVK*FIIYSGRSSLVEQAQENVIK*FYIF*F ILYLTCHG*IHSLNM*Y*T*KKKKK

Frame 2

LQQNESNRSCSGDCFCQYPESVLRLRNPG*KEH FIKENVSTSYPAPKTPIGVAVGV**ASMLASPP VSPCFRMRKMKVASPEMTSSLKSGPSANTLNSA LRMLLGPLSSKEVAIPTKPCMCEYLLIQNLISSY T**PRNGSWSFPSFSSLSMGACRTLNSSQNSSK SLGKGSSKQQ*QLERGYSLEGLTQVLFVMLAMP *RIMPLSLEERYAP*VLPPGELWKTRRTSLEEML SGHTRPCPIP*ASSLFSTACIPTSFWLTTGPLE NMEQR*NFEDNWKSIFHSRR*TQESVKVFLWWH S*WKEDPM*SRLFWSTFETPLPCQWLSVMGVDGH RTSWPLGINTQKKAD**MNL*GTSCW*LYRRLS HTLEPKLSICSSSSWSA*RRRN*LRYFGWDQKD TRTLIWLS*QLYSKEPMPRPQTN*A*L*PGTEST SLAARSLFTGNSGRWDLWSKPCWMP*FWTEWIL *NYS*RME*ACTVFSPSPD*RNCTIRDMGPQIH CTTWSGMSKSESIQVSVGSILRGTCPQTTESA*L TSAW*SST*WAGLIAATTRASASGPSTTTSSAP RGPKP*NCWEWRMIFP*GEEERQPRNVKKRWTL TWMILRSTTSPSLSMSSWCGLFS*SGRRWPCSSG STVRRPWPRPWWPASSAKPWLMRPLRTTWLTTF PRS*ITIPETLASWLWSSWTSPTSRTNSWP*NC *RMS*RTGATPRACSLPWLPNTATSSRTRAARCC SPTCGWAGSACARTQASR*FWEFYFLLQFSAWS SRTKTTCPICLRPRKSTSKRRRQKNQRSPQRKK RKRTWSSQQCWDETTGSPPGRRMKRKFRASTG*S PSAEKSMNSTMHPS*SSGSTHWRISDT*CSSTI SC**RWNAGRPPRNGS*SPIFSPWE*KR*ERF* CQSQGSCYRK*RYGCRSTGMSRTSSPSFCFLSE* SFVSKTSPSGVTGGSSTA*TSFTGISVS*TSSA *TSIWARM***LEK***T*CTLSSLCWWF**AL GSPGKPSFFPMRSHHGNWPRTSSTCPIG*FMGKC LRTR*TLPVDRMRPERMVK*SSCLPARQELGSC RPSWPATS*WQTSCWSTSSLLSLTIHFLK*NRY PTKSGSFRGISSS*LSMKGQFCPHH*SSSAT*P* YSSTCAADGGNTRATRMKGTTA*NSS*PMMSSR KYMTLKSNA*KNTSEKRMIGSTHLMMRGYG*LQ KGWRTCLCGWRKSTRESTP*RLHSRPWTSGWRSW KTLSGAWPRPWSA*QVWSGPSPTKSARGPRQTA RTPPTLSVRAASTARKGTPSSSKRV*TLQVRRP CPQLLQP*CPVCEAILSIRSI*KTKVV*KSWKVF LKKGP*AYTGLLVPTL*QKNPKLLQPLPTPWPL FLIPEDHHRV*TSMSLLWMSSTVI*TLWTIP*T SLGWASQAFQLQYLPQPLQVVPMQHLHPQTDLQA GALILRTSPPWTLDLFLQTTPTSQNAKTPGTQS LRCTTPLSVPKVAAT*PPHPFF*KRLPL*NLIA LCFPPQGAIMPTLGCL*KQQNTQVLQTVLTQGVS MPLKQLRTELPSLEVLETKWRT*LAAIQSEKQN *VTPALTVRRMRPKAAEPPLQYPPRRVITQREP CPTTSLFPR*SAPTATRQRSQVRHMHTPGRASPS VTNSTGSGTQQACEIPSREASPPSRRAEGTACP *GDCPEHRLSKALKASTTKPS*YPPQKAQESSP KILSNSSFSPFLESYLLYS*LSKTSNALGGVNSK VTSGPQIKKAFDLTQCQTQGI*GMFTLAGQGGG REGEGRVRDECVSAVTAESHESRGNKGLRARSM PGGIC*FLTIIKSCRMQG*IAK*NKIASVHNEIF *TSILFSFHIGSITGD**RASSLFSISRQQAVL PNVPHPSHANTSVIDHYKEKTR*QS*FTCLLSI HFWCHNCLSFFEENKGTEMPFCIAIEMKEELMLK KQKSSDLLYTVGVQV*SSKLRRM*LNNFIFFNL FCISPVMDEFIH*ICNIELKKKKKK

Sequence NM_206945

AGCTGCAGCAAAATGAAAGCAACAGGAGCTGCTCCGGGGACTGCTTTTGCCAGTACCCAGAATCAGTGCTCAGGCTCAGAAATCCTGGATAGAAAGAGCA TTTTATAAAAGAGAATGTGTCCACATCATACCCAGCACCAAAGACCCCCATAGGTGTTGCTGTGGGCGTCTGATAGGCCAGCATGTTGGCCTCACCCCCA GTATCTCCGTGCTTCAGAATGAGAAAAATGAAAGTCGCCTCTCCCGAAATGACATCCAGTCTGAAAAGTGGTCCATCAGCAAACACACTCAACTCAGCCC TACGGATGCTTTTGGGACCATTGAGTTCCAAGGAGGTGGCCATTCCAACAAAGCCATGTATGTGCGAGTATCTTTTGATACAAAACCTGATCTCCTCTTA CACCTGATGACCAAGGAATGGCAGTTGGAGCTTCCCAAGCTTCTCATCTCTGTCCATGGGGGCCTGCAGAACTTTGAACTCCAGCCAAAACTCAAGCAAG TCTTTGGGAAAGGGCTCATCAAAGCAGCAATGACAACTGGAGCGTGGATATTCACTGGAGGGGTTAACACAGGTGTTATTCGTCATGTTGGCGATGCCTT GAAGGATCATGCCTCTAAGTCTCGAGGAAAGATATGCACCATAGGTATTGCCCCCTGGGGAATTGTGGAAAACCAGGAGGACCTCATTGGAAGAGATGTT GTCCGGCCATACCAGACCATGTCCAATCCCATGAGCAAGCTCACTGTTCTCAACAGCATGCATTCCCACTTCATTCTGGCTGACAACGGGACCACTGGAA AATATGGAGCAGAGGTGAAACTTCGAAGACAACTGGAAAAGCATATTTCACTCCAGAAGATAAACACAAGAATCGGTCAAGGTGTTCCTGTGGTGGCACT CATAGTGGAAGGAGGACCCAATGTGATCTCGATTGTTTTGGAGTACCTTCGAGACACCCCTCCCGTGCCAGTGGTTGTCTGTGATGGGAGTGGACGGGCA TCGGACATCCTGGCCTTTGGGCATAAATACTCAGAAGAAGGCGGACTGATAAATGAATCTTTGAGGGACCAGCTGTTGGTGACTATACAGAAGACTTTCA CATACACTCGAACCCAAGCTCAGCATCTGTTCATCATCCTCATGGAGTGCATGAAGAAGAAGGAATTGATTACGGTATTTCGGATGGGATCAGAAGGACA CCAGGACATTGATTTGGCTATCCTGACAGCTTTACTCAAAGGAGCCAATGCCTCGGCCCCAGACCAACTGAGCTTAGCTTTAGCCTGGAACAGAGTCGAC ATCGCTCGCAGCCAGATCTTTATTTACGGGCAACAGTGGCCGGTGGGATCTCTGGAGCAAGCCATGTTGGATGCCTTAGTTCTGGACAGAGTGGATTTTG TGAAATTACTCATAGAGAATGGAGTAAGCATGCACCGTTTTCTCACCATCTCCAGACTAGAGGAATTGTACAATACGAGACATGGGCCCTCAAATACATT GTACCACTTGGTCAGGGATGTCAAAAAGCGAGAGTATCCAGGTTTCGGTTGGATCTATTTTAAGGGGAACCTGCCCCCAGACTACAGAATCAGCCTGATT GACATCGGCCTGGTGATCGAGTACCTGATGGGCGGGGCTTATCGCTGCAACTACACGCGCAAGCGCTTCCGGACCCTCTACCACAACCTCTTCGGCCCCA AGAGGGATGATATTCCCTTGAGGCGAGGAAGAAAGACAACCAAGAAACGTGAAGAAGAGGTGGACATTGACTTGGATGATCCTGAGATCAACCACTTCCC CTTCCCTTTCCATGAGCTCATGGTGTGGGCTGTTCTCATGAAGCGGCAGAAGATGGCCCTGTTCTTCTGGCAGCACGGTGAGGAGGCCATGGCCAAGGCC CTGGTGGCCTGCAAGCTCTGCAAAGCCATGGCTCATGAGGCCTCTGAGAACGACATGGTTGACGACATTTCCCAGGAGCTGAATCACAATTCCAGAGACT TTGGCCAGCTGGCTGTGGAGCTCCTGGACCAGTCCTACAAGCAGGACGAACAGCTGGCCATGAAACTGCTGACGTATGAGCTGAAGAACTGGAGCAACGC CACGTGCCTGCAGCTTGCCGTGGCTGCCAAACACCGCGACTTCATCGCGCACACGTGCAGCCAGATGCTGCTCACCGACATGTGGATGGGCCGGCTCCGC ATGCGCAAGAACTCAGGCCTCAAGGTAATTCTGGGAATTCTACTTCCTCCTTCAATTCTCAGCTTGGAGTTCAAGAACAAAGACGACATGCCCTATATGT CTCAGGCCCAGGAAATCCACCTCCAAGAGAAGGAGGCAGAAGAACCAGAGAAGCCCACAAAGGAAAAAGAGGAAGAGGACATGGAGCTCACAGCAATGTT GGGACGAAACAACGGGGAGTCCTCCAGGAAGAAGGATGAAGAGGAAGTTCAGAGCAAGCACCGGTTAATCCCCCTCGGCAGAAAAATCTATGAATTCTAC AATGCACCCATCGTGAAGTTCTGGTTCTACACACTGGCGTATATCGGATACCTGATGCTCTTCAACTATATCGTGTTAGTGAAGATGGAACGCTGGCCGT CCACCCAGGAATGGATCGTAATCTCCTATATTTTCACCCTGGGAATAGAAAAGATGAGAGAGATTCTGATGTCAGAGCCAGGGAAGTTGCTACAGAAAGT GAAGGTATGGCTGCAGGAGTACTGGAATGTCACGGACCTCATCGCCATCCTTCTGTTTTCTGTCGGAATGATCCTTCGTCTCCAAGACCAGCCCTTCAGG AGTGACGGGAGGGTCATCTACTGCGTGAACATCATTTACTGGTATATCCGTCTCCTAGACATCTTCGGCGTGAACAAGTATTTGGGCCCGTATGTAATGA TGATTGGAAAAATGATGATAGACATGATGTACTTTGTCATCATTATGCTGGTGGTTCTGATGAGCTTTGGGGTCGCCAGGCAAGCCATCCTTTTTCCCAA TGAGGAGCCATCATGGAAACTGGCCAAGAACATCTTCTACATGCCCTATTGGATGATTTATGGGGAAGTGTTTGCGGACCAGATAGACCCTCCCTGTGGA CAGAATGAGACCCGAGAGGATGGTAAAATAATCCAGCTGCCTCCCTGCAAGACAGGAGCTTGGATCGTGCCGGCCATCATGGCCTGCTACCTCTTAGTGG CAAACATCTTGCTGGTCAACCTCCTCATTGCTGTCTTTAACAATACATTTTTTGAAGTAAAATCGATATCCAACCAAGTCTGGAAGTTTCAGAGGTATCA GCTCATCATGACTTTCCATGAAAGGCCAGTTCTGCCCCCACCACTGATCATCTTCAGCCACATGACCATGATATTCCAGCACCTGTGCTGCCGATGGAGG AAACACGAGAGCGACCCGGATGAAAGGGACTACGGCCTGAAACTCTTCATAACCGATGATGAGCTCAAGAAAGTACATGACTTTGAAGAGCAATGCATAG AAGAATACTTCAGAGAAAAGGATGATCGGTTCAACTCATCTAATGATGAGAGGATACGGGTGACTTCAGAAAGGGTGGAGAACATGTCTATGCGGCTGGA GGAAGTCAACGAGAGAGAGCACTCCATGAAGGCTTCACTCCAGACCGTGGACATCCGGCTGGCGCAGCTGGAAGACCTTATCGGGCGCATGGCCACGGCC CTGGAGCGCCTGACAGGTCTGGAGCGGGCCGAGTCCAACAAAATCCGCTCGAGGACCTCGTCAGACTGCACGGACGCCGCCTACATTGTCCGTCAGAGCA GCTTCAACAGCCAGGAAGGGAACACCTTCAAGCTCCAAGAGAGTATAGACCCTGCAGGTGAGGAGACCATGTCCCCAACTTCTCCAACCTTAATGCCCCG TATGCGAAGCCATTCTTTCTATTCGGTCAATATGAAAGACAAAGGTGGTATAGAAAAGTTGGAAAGTATTTTTAAAGAAAGGTCCCTGAGCCTACACCGG GCTACTAGTTCCCACTCTGTAGCAAAAGAACCCAAAGCTCCTGCAGCCCCTGCCAACACCTTGGCCATTGTTCCTGATTCCAGAAGACCATCATCGTGTA TAGACATCTATGTCTCTGCTATGGATGAGCTCCACTGTGATATAGACCCTCTGGACAATTCCGTGAACATCCTTGGGCTGGGCGAGCCAAGCTTTTCAAC TCCAGTACCTTCCACAGCCCCTTCAAGTAGTGCCTATGCAACACTTGCACCCACAGACAGACCTCCAAGCCGGAGCATTGATTTTGAGGACATCACCTCC ATGGACACTAGATCTTTTTCTTCAGACTACACCCACCTCCCAGAATGCCAAAACCCCTGGGACTCAGAGCCTCCGATGTACCACACCATTGAGCGTTCCA AAAGTAGCCGCTACCTAGCCACCACACCCTTTCTTCTAGAAGAGGCTCCCATTGTGAAATCTCATAGCTTTATGTTTTCCCCCTCAAGGAGCTATTATGC CAACTTTGGGGTGCCTGTAAAAACAGCAGAATACACAAGTATTACAGACTGTATTGACACAAGGTGTGTCAATGCCCCTCAAGCAATTGCGGACAGAGCT GCCTTCCCTGGAGGTCTTGGAGACAAAGTGGAGGACTTAACTTGCTGCCATCCAGAGCGAGAAGCAGAACTGAGTCACCCCAGCTCTGACAGTGAGGAGA ATGAGGCCAAAGGCCGCAGAGCCACCATTGCAATATCCTCCCAGGAGGGTGATAACTCAGAGAGAACCCTGTCCAACAACATCACTGTTCCCAAGATAGA GCGCGCCAACAGCTACTCGGCAGAGGAGCCAAGTGCGCCATATGCACACACCAGGAAGAGCTTCTCCATCAGTGACAAACTCGACAGGCAGCGGAACACA GCAAGCCTGCGAAATCCCTTCCAGAGAAGCAAGTCCTCCAAGCCGGAGGGCCGAGGGGACAGCCTGTCCATGAGGAGACTGTCCAGAACATCGGCTTTCC AAAGCTTTGAAAGCAAGCACAACTAAACCTTCTTAATATCCGCCACAGAAGGCTCAAGAATCCAGCCCTAAAATTCTCTCCAACTCCAGTTTTTCCCCTT TCCTTGAATCATACCTGCTTTATTCTTAGCTGAGCAAAACAAGCAATGCTTTGGGAGGTGTTAACTCAAAGGTGACTTCTGGGCCACAGATCAAGAAAGC ATTTGATCTGACCCAGTGCCAGACACAGGGGATTTAAGGCATGTTCACACTTGCTGGGCAGGGAGGGGGAAGAGAGGGAGAAGGAAGGGTTAGAGATGAA TGTGTATCCGCAGTCACAGCAGAAAGCCATGAGAGCAGGGGAAACAAGGGGCTTCGAGCACGCTCCATGCCAGGAGGCATCTGTTGATTTCTGACCATTA TCAAGAGTTGTAGGATGCAGGGCTAAATTGCAAAATAAAATAAAATAGCCAGCGTACACAATGAGATATTCTAAACTTCCATTCTGTTTTCTTTTCACAT TGGCTCCATCACTGGTGACTGATGAAGAGCATCCTCTTTATTCAGTATAAGCCGGCAGCAAGCAGTTCTACCTAACGTCCCACATCCTTCTCATGCCAAC ACTTCTGTAATTGATCATTATAAAGAAAAAACAAGGTAACAGTCATAGTTCACCTGTCTCTTATCTATTCACTTCTGGTGCCACAACTGTTTATCCTTTT TTGAAGAAAATAAGGGAACAGAAATGCCTTTTTGTATTGCAATCGAAATGAAAGAAGAGTTGATGTTAAAAAAACAAAAGTCAAGTGATTTATTATATAC AGTGGGCGTTCAAGTCTAGTCGAGCAAGCTCAGGAGAATGTAATTAAATAATTTTATATTTTTTAATTTATTTTGTATCTCACCTGTCATGGATGAATTC ATTCACTGAATATGTAATATTGAACTTAAAAAAAAAAAAAAAAAAA

refseq_TRPM3.F2 (forward primer)
refseq_TRPM3.R2 (reverse primer)
Single exon skipping

Frame 0

SCSKMKATGAAPGTAFASTQNQCSGSEILDRKS IL*KRMCPHHTQHQRPP*VLLWASDRPACWPHPQ YLRASE*EK*KSPLPK*HPV*KVVHQQTHSTQP YGCFWDH*VPRRWPFQQSHVCASIF*YKT*SPL TPDDQGMAVGASQASHLCPWGPAEL*TPAKTQAS LWERAHQSSNDNWSVDIHWRG*HRCYSSCWRCL EGSCL*VSRKDMHHRYCPLGNCGKPGGPHWKRC CPAIPDHVQSHEQAHCSQQHAFPLHSG*QRDHWK IWSRGETSKTTGKAYFTPEDKHKNRSRCSCGGT HSGRRTQCDLDCFGVPSRHPSRASGCL*WEWTG IGHPGLWA*ILRRRRTDK*IFEGPAVGDYTEDFH IHSNPSSASVHHPHGVHEEEGIDYGISDGIRRT PGH*FGYPDSFTQRSQCLGPRPTELSFSLEQSR HRSQPDLYLRATVAGGISGASHVGCLSSGQSGFC EITHREWSKHAPFSHHLQTRGIVQYETWALKYI VPLGQGCQKARVSRFRLDLF*GEPAPRLQNQPD *HRPGDRVPDGRGLSLQLHAQALPDPLPQPLRPQ EG*YSLEARKKDNQET*RRGGH*LG*S*DQPLP LPFP*AHGVGCSHEAAEDGPVLLAAR*GGHGQG PGGLQALQSHGS*GL*ERHG*RHFPGAESQFQRL WPAGCGAPGPVLQAGRTAGHETADV*AEELEQR HVPAACRGCQTPRLHRAHVQPDAAHRHVDGPAP HAQELRPQGNSGNSTSSFNSQLGVQEQRRHALYV SGPGNPPPREGGRRTREAHKGKRGRGHGAHSNV GTKQRGVLQEEG*RGSSEQAPVNPPRQKNL*IL QCTHREVLVLHTGVYRIPDALQLYRVSEDGTLAV HPGMDRNLLYFHPGNRKDERDSDVRAREVATES EGMAAGVLECHGPHRHPSVFCRNDPSSPRPALQ E*REGHLLREHHLLVYPSPRHLRREQVFGPVCND DWKNDDRHDVLCHHYAGGSDELWGRQASHPFSQ *GAIMETGQEHLLHALLDDLWGSVCGPDRPSLW TE*DPRGW*NNPAASLQDRSLDRAGHHGLLPLSG KHLAGQPPHCCL*QYIF*SKIDIQPSLEVSEVS AHHDFP*KASSAPTTDHLQPHDHDIPAPVLPME ETRERPG*KGLRPETLHNR**AQEST*L*RAMHR RILQRKG*SVQLI***EDTGDFRKGGEHVYAAG GSQRERALHEGFTPDRGHPAGAAGRPYRAHGHG PGAPDRSGAGRVQQNPLEDLVRLHGRRLHCPSEQ LQQPGREHLQAPREYRPCR*GDHVPNFSNLNAP YAKPFFLFGQYERQRWYRKVGKYF*RKVPEPTP GY*FPLCSKRTQSSCSPCQHLGHCS*FQKTIIVY RHLCLCYG*APL*YRPSGQFREHPWAGRAKLFN SSTFHSPFK*CLCNTCTHRQTSKPEH*F*GHHL HGH*IFFFRLHPPPRMPKPLGLRASDVPHH*AFQ K*PLPSHHTLSSRRGSHCEIS*LYVFPLKELLC QLWGACKNSRIHKYYRLY*HKVCQCPSSNCGQS CLPWRSWRQSGGLNLLPSRARSRTESPQL*Q*GE *GQRPQSHHCNILPGG**LRENPVQQHHCSQDR ARQQLLGRGAKCAICTHQEELLHQ*QTRQAAEH SKPAKSLPEKQVLQAGGPRGQPVHEETVQNIGFP KL*KQAQLNLLNIRHRRLKNPALKFSPTPVFPL SLNHTCFILS*AKQAMLWEVLTQR*LLGHRSRK HLI*PSARHRGFKACSHLLGREGEEREKEGLEMN VYPQSQQKAMRAGETRGFEHAPCQEASVDF*PL SRVVGCRAKLQNKIK*PAYTMRYSKLPFCFLFT LAPSLVTDEEHPLYSV*AGSKQFYLTSHILLMPT LL*LIIIKKKQGNSHSSPVSYLFTSGATTVYPF LKKIREQKCLFVLQSK*KKS*C*KNKSQVIYYI QWAFKSSRASSGECN*IILYFLIYFVSHLSWMNS FTEYVILNLKKKKKK

Frame 1

AAAK*KQQELLRGLLLPVPRISAQAQKSWIERA FYKRECVHIIPSTKDPHRCCCGRLIGQHVGLTP SISVLQNEKNESRLSRNDIQSEKWSISKHTQLSP TDAFGTIEFQGGGHSNKAMYVRVSFDTKPDLLL HLMTKEWQLELPKLLISVHGGLQNFELQPKLKQ VFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDAL KDHASKSRGKICTIGIAPWGIVENQEDLIGRDV VRPYQTMSNPMSKLTVLNSMHSHFILADNGTTG KYGAEVKLRRQLEKHISLQKINTRIGQGVPVVAL IVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRA SDILAFGHKYSEEGGLINESLRDQLLVTIQKTF TYTRTQAQHLFIILMECMKKKELITVFRMGSEGH QDIDLAILTALLKGANASAPDQLSLALAWNRVD IARSQIFIYGQQWPVGSLEQAMLDALVLDRVDF VKLLIENGVSMHRFLTISRLEELYNTRHGPSNTL YHLVRDVKKREYPGFGWIYFKGNLPPDYRISLI DIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGP KRDDIPLRRGRKTTKKREEEVDIDLDDPEINHFP FPFHELMVWAVLMKRQKMALFFWQHGEEAMAKA LVACKLCKAMAHEASENDMVDDISQELNHNSRD FGQLAVELLDQSYKQDEQLAMKLLTYELKNWSNA TCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLR MRKNSGLKVILGILLPPSILSLEFKNKDDMPYM SQAQEIHLQEKEAEEPEKPTKEKEEEDMELTAML GRNNGESSRKKDEEEVQSKHRLIPLGRKIYEFY NAPIVKFWFYTLAYIGYLMLFNYIVLVKMERWP STQEWIVISYIFTLGIEKMREILMSEPGKLLQKV KVWLQEYWNVTDLIAILLFSVGMILRLQDQPFR SDGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVM MIGKMMIDMMYFVIIMLVVLMSFGVARQAILFPN EEPSWKLAKNIFYMPYWMIYGEVFADQIDPPCG QNETREDGKIIQLPPCKTGAWIVPAIMACYLLV ANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQ LIMTFHERPVLPPPLIIFSHMTMIFQHLCCRWR KHESDPDERDYGLKLFITDDELKKVHDFEEQCI EEYFREKDDRFNSSNDERIRVTSERVENMSMRLE EVNEREHSMKASLQTVDIRLAQLEDLIGRMATA LERLTGLERAESNKIRSRTSSDCTDAAYIVRQS SFNSQEGNTFKLQESIDPAGEETMSPTSPTLMPR MRSHSFYSVNMKDKGGIEKLESIFKERSLSLHR ATSSHSVAKEPKAPAAPANTLAIVPDSRRPSSC IDIYVSAMDELHCDIDPLDNSVNILGLGEPSFST PVPSTAPSSSAYATLAPTDRPPSRSIDFEDITS MDTRSFSSDYTHLPECQNPWDSEPPMYHTIERS KSSRYLATTPFLLEEAPIVKSHSFMFSPSRSYYA NFGVPVKTAEYTSITDCIDTRCVNAPQAIADRA AFPGGLGDKVEDLTCCHPEREAELSHPSSDSEE NEAKGRRATIAISSQEGDNSERTLSNNITVPKIE RANSYSAEEPSAPYAHTRKSFSISDKLDRQRNT ASLRNPFQRSKSSKPEGRGDSLSMRRLSRTSAF QSFESKHN*TFLISATEGSRIQP*NSLQLQFFPF P*IIPALFLAEQNKQCFGRC*LKGDFWATDQES I*SDPVPDTGDLRHVHTCWAGRGKRGRRKG*R* MCIRSHSRKP*EQGKQGASSTLHARRHLLISDHY QEL*DAGLNCKIK*NSQRTQ*DILNFHSVFFSH WLHHW*LMKSILFIQYKPAASSST*RPTSFSCQ HFCN*SL*RKNKVTVIVHLSLIYSLLVPQLFILF *RK*GNRNAFLYCNRNERRVDVKKTKVK*FIIY SGRSSLVEQAQENVIK*FYIF*FILYLTCHG*I HSLNM*Y*T*KKKKK

Frame 2

LQQNESNRSCSGDCFCQYPESVLRLRNPG*KEH FIKENVSTSYPAPKTPIGVAVGV**ASMLASPP VSPCFRMRKMKVASPEMTSSLKSGPSANTLNSA LRMLLGPLSSKEVAIPTKPCMCEYLLIQNLISSY T**PRNGSWSFPSFSSLSMGACRTLNSSQNSSK SLGKGSSKQQ*QLERGYSLEGLTQVLFVMLAMP *RIMPLSLEERYAP*VLPPGELWKTRRTSLEEML SGHTRPCPIP*ASSLFSTACIPTSFWLTTGPLE NMEQR*NFEDNWKSIFHSRR*TQESVKVFLWWH S*WKEDPM*SRLFWSTFETPLPCQWLSVMGVDGH RTSWPLGINTQKKAD**MNL*GTSCW*LYRRLS HTLEPKLSICSSSSWSA*RRRN*LRYFGWDQKD TRTLIWLS*QLYSKEPMPRPQTN*A*L*PGTEST SLAARSLFTGNSGRWDLWSKPCWMP*FWTEWIL *NYS*RME*ACTVFSPSPD*RNCTIRDMGPQIH CTTWSGMSKSESIQVSVGSILRGTCPQTTESA*L TSAW*SST*WAGLIAATTRASASGPSTTTSSAP RGMIFP*GEEERQPRNVKKRWTLTWMILRSTTS PSLSMSSWCGLFS*SGRRWPCSSGSTVRRPWPRP WWPASSAKPWLMRPLRTTWLTTFPRS*ITIPET LASWLWSSWTSPTSRTNSWP*NC*RMS*RTGAT PRACSLPWLPNTATSSRTRAARCCSPTCGWAGSA CARTQASR*FWEFYFLLQFSAWSSRTKTTCPIC LRPRKSTSKRRRQKNQRSPQRKKRKRTWSSQQC WDETTGSPPGRRMKRKFRASTG*SPSAEKSMNST MHPS*SSGSTHWRISDT*CSSTISC**RWNAGR PPRNGS*SPIFSPWE*KR*ERF*CQSQGSCYRK *RYGCRSTGMSRTSSPSFCFLSE*SFVSKTSPSG VTGGSSTA*TSFTGISVS*TSSA*TSIWARM** *LEK***T*CTLSSLCWWF**ALGSPGKPSFFP MRSHHGNWPRTSSTCPIG*FMGKCLRTR*TLPVD RMRPERMVK*SSCLPARQELGSCRPSWPATS*W QTSCWSTSSLLSLTIHFLK*NRYPTKSGSFRGI SSS*LSMKGQFCPHH*SSSAT*P*YSSTCAADGG NTRATRMKGTTA*NSS*PMMSSRKYMTLKSNA* KNTSEKRMIGSTHLMMRGYG*LQKGWRTCLCGW RKSTRESTP*RLHSRPWTSGWRSWKTLSGAWPRP WSA*QVWSGPSPTKSARGPRQTARTPPTLSVRA ASTARKGTPSSSKRV*TLQVRRPCPQLLQP*CP VCEAILSIRSI*KTKVV*KSWKVFLKKGP*AYTG LLVPTL*QKNPKLLQPLPTPWPLFLIPEDHHRV *TSMSLLWMSSTVI*TLWTIP*TSLGWASQAFQ LQYLPQPLQVVPMQHLHPQTDLQAGALILRTSPP WTLDLFLQTTPTSQNAKTPGTQSLRCTTPLSVP KVAAT*PPHPFF*KRLPL*NLIALCFPPQGAIM PTLGCL*KQQNTQVLQTVLTQGVSMPLKQLRTEL PSLEVLETKWRT*LAAIQSEKQN*VTPALTVRR MRPKAAEPPLQYPPRRVITQREPCPTTSLFPR* SAPTATRQRSQVRHMHTPGRASPSVTNSTGSGTQ QACEIPSREASPPSRRAEGTACP*GDCPEHRLS KALKASTTKPS*YPPQKAQESSPKILSNSSFSP FLESYLLYS*LSKTSNALGGVNSKVTSGPQIKKA FDLTQCQTQGI*GMFTLAGQGGGREGEGRVRDE CVSAVTAESHESRGNKGLRARSMPGGIC*FLTI IKSCRMQG*IAK*NKIASVHNEIF*TSILFSFHI GSITGD**RASSLFSISRQQAVLPNVPHPSHAN TSVIDHYKEKTR*QS*FTCLLSIHFWCHNCLSF FEENKGTEMPFCIAIEMKEELMLKKQKSSDLLYT VGVQV*SSKLRRM*LNNFIFFNLFCISPVMDEF IH*ICNIELKKKKKK