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Sequences

Sequence NM_004176

AGCAGAGCTGCGGCCGGGGGAACCCAGTTTCCGAGGAACTTTTCGCCGGCGCCGGGCCGCCTCTGAGGCCAGGGCAGGACACGAACGCGCGGAGCGGCGG
CGGCGACTGAGAGCCGGGGCCGCGGCGGCGCTCCCTAGGAAGGGCCGTACGAGGCGGCGGGCCCGGCGGGCCTCCCGGAGGAGGCGGCTGCGCCATGGAC
GAGCCACCCTTCAGCGAGGCGGCTTTGGAGCAGGCGCTGGGCGAGCCGTGCGATCTGGACGCGGCGCTGCTGACCGACATCGAAGACATGCTTCAGCTTA
TCAACAACCAAGACAGTGACTTCCCTGGCCTATTTGACCCACCCTATGCTGGGAGTGGGGCAGGGGGCACAGACCCTGCCAGCCCCGATACCAGCTCCCC
AGGCAGCTTGTCTCCACCTCCTGCCACATTGAGCTCCTCTCTTGAAGCCTTCCTGAGCGGGCCGCAGGCAGCGCCCTCACCCCTGTCCCCTCCCCAGCCT
GCACCCACTCCATTGAAGATGTACCCGTCCATGCCCGCTTTCTCCCCTGGGCCTGGTATCAAGGAAGAGTCAGTGCCACTGAGCATCCTGCAGACCCCCA
CCCCACAGCCCCTGCCAGGGGCCCTCCTGCCACAGAGCTTCCCAGCCCCAGCCCCACCGCAGTTCAGCTCCACCCCTGTGTTAGGCTACCCCAGCCCTCC
GGGAGGCTTCTCTACAGGAAGCCCTCCCGGGAACACCCAGCAGCCGCTGCCTGGCCTGCCACTGGCTTCCCCGCCAGGGGTCCCGCCCGTCTCCTTGCAC
ACCCAGGTCCAGAGTGTGGTCCCCCAGCAGCTACTGACAGTCACAGCTGCCCCCACGGCAGCCCCTGTAACGACCACTGTGACCTCGCAGATCCAGCAGG
TCCCGGTCCTGCTGCAGCCCCACTTCATCAAGGCAGACTCGCTGCTTCTGACAGCCATGAAGACAGACGGAGCCACTGTGAAGGCGGCAGGTCTCAGTCC
CCTGGTCTCTGGCACCACTGTGCAGACAGGGCCTTTGCCGACCCTGGTGAGTGGCGGAACCATCTTGGCAACAGTCCCACTGGTCGTAGATGCGGAGAAG
CTGCCTATCAACCGGCTCGCAGCTGGCAGCAAGGCCCCGGCCTCTGCCCAGAGCCGTGGAGAGAAGCGCACAGCCCACAACGCCATTGAGAAGCGCTACC
GCTCCTCCATCAATGACAAAATCATTGAGCTCAAGGATCTGGTGGTGGGCACTGAGGCAAAGCTGAATAAATCTGCTGTCTTGCGCAAGGCCATCGACTA
CATTCGCTTTCTGCAACACAGCAACCAGAAACTCAAGCAGGAGAACCTAAGTCTGCGCACTGCTGTCCACAAAAGCAAATCTCTGAAGGATCTGGTGTCG
GCCTGTGGCAGTGGAGGGAACACAGACGTGCTCATGGAGGGCGTGAAGACTGAGGTGGAGGACACACTGACCCCACCCCCCTCGGATGCTGGCTCACCTT
TCCAGAGCAGCCCCTTGTCCCTTGGCAGCAGGGGCAGTGGCAGCGGTGGCAGTGGCAGTGACTCGGAGCCTGACAGCCCAGTCTTTGAGGACAGCAAGGC
AAAGCCAGAGCAGCGGCCGTCTCTGCACAGCCGGGGCATGCTGGACCGCTCCCGCCTGGCCCTGTGCACGCTCGTCTTCCTCTGCCTGTCCTGCAACCCC
TTGGCCTCCTTGCTGGGGGCCCGGGGGCTTCCCAGCCCCTCAGATACCACCAGCGTCTACCATAGCCCTGGGCGCAACGTGCTGGGCACCGAGAGCAGAG
ATGGCCCTGGCTGGGCCCAGTGGCTGCTGCCCCCAGTGGTCTGGCTGCTCAATGGGCTGTTGGTGCTCGTCTCCTTGGTGCTTCTCTTTGTCTACGGTGA
GCCAGTCACACGGCCCCACTCAGGCCCCGCCGTGTACTTCTGGAGGCATCGCAAGCAGGCTGACCTGGACCTGGCCCGGGGAGACTTTGCCCAGGCTGCC
CAGCAGCTGTGGCTGGCCCTGCGGGCACTGGGCCGGCCCCTGCCCACCTCCCACCTGGACCTGGCTTGTAGCCTCCTCTGGAACCTCATCCGTCACCTGC
TGCAGCGTCTCTGGGTGGGCCGCTGGCTGGCAGGCCGGGCAGGGGGCCTGCAGCAGGACTGTGCTCTGCGAGTGGATGCTAGCGCCAGCGCCCGAGACGC
AGCCCTGGTCTACCATAAGCTGCACCAGCTGCACACCATGGGGAAGCACACAGGCGGGCACCTCACTGCCACCAACCTGGCGCTGAGTGCCCTGAACCTG
GCAGAGTGTGCAGGGGATGCCGTGTCTGTGGCGACGCTGGCCGAGATCTATGTGGCGGCTGCATTGAGAGTGAAGACCAGTCTCCCACGGGCCTTGCATT
TTCTGACACGCTTCTTCCTGAGCAGTGCCCGCCAGGCCTGCCTGGCACAGAGTGGCTCAGTGCCTCCTGCCATGCAGTGGCTCTGCCACCCCGTGGGCCA
CCGTTTCTTCGTGGATGGGGACTGGTCCGTGCTCAGTACCCCATGGGAGAGCCTGTACAGCTTGGCCGGGAACCCAGTGGACCCCCTGGCCCAGGTGACT
CAGCTATTCCGGGAACATCTCTTAGAGCGAGCACTGAACTGTGTGACCCAGCCCAACCCCAGCCCTGGGTCAGCTGATGGGGACAAGGAATTCTCGGATG
CCCTCGGGTACCTGCAGCTGCTGAACAGCTGTTCTGATGCTGCGGGGGCTCCTGCCTACAGCTTCTCCATCAGTTCCAGCATGGCCACCACCACCGGCGT
AGACCCGGTGGCCAAGTGGTGGGCCTCTCTGACAGCTGTGGTGATCCACTGGCTGCGGCGGGATGAGGAGGCGGCTGAGCGGCTGTGCCCGCTGGTGGAG
CACCTGCCCCGGGTGCTGCAGGAGTCTGAGAGACCCCTGCCCAGGGCAGCTCTGCACTCCTTCAAGGCTGCCCGGGCCCTGCTGGGCTGTGCCAAGGCAG
AGTCTGGTCCAGCCAGCCTGACCATCTGTGAGAAGGCCAGTGGGTACCTGCAGGACAGCCTGGCTACCACACCAGCCAGCAGCTCCATTGACAAGGCCGT
GCAGCTGTTCCTGTGTGACCTGCTTCTTGTGGTGCGCACCAGCCTGTGGCGGCAGCAGCAGCCCCCGGCCCCGGCCCCAGCAGCCCAGGGCACCAGCAGC
AGGCCCCAGGCTTCCGCCCTTGAGCTGCGTGGCTTCCAACGGGACCTGAGCAGCCTGAGGCGGCTGGCACAGAGCTTCCGGCCCGCCATGCGGAGGGTGT
TCCTACATGAGGCCACGGCCCGGCTGATGGCGGGGGCCAGCCCCACACGGACACACCAGCTCCTCGACCGCAGTCTGAGGCGGCGGGCAGGCCCCGGTGG
CAAAGGAGGCGCGGTGGCGGAGCTGGAGCCGCGGCCCACGCGGCGGGAGCACGCGGAGGCCTTGCTGCTGGCCTCCTGCTACCTGCCCCCCGGCTTCCTG
TCGGCGCCCGGGCAGCGCGTGGGCATGCTGGCTGAGGCGGCGCGCACACTCGAGAAGCTTGGCGATCGCCGGCTGCTGCACGACTGTCAGCAGATGCTCA
TGCGCCTGGGCGGTGGGACCACTGTCACTTCCAGCTAGACCCCGTGTCCCCGGCCTCAGCACCCCTGTCTCTAGCCACTTTGGTCCCGTGCAGCTTCTGT
CCTGCGTCGAAGCTTTGAAGGCCGAAGGCAGTGCAAGAGACTCTGGCCTCCACAGTTCGACCTGCGGCTGCTGTGTGCCTTCGCGGTGGAAGGCCCGAGG
GGCGCGATCTTGACCCTAAGACCGGCGGCCATGATGGTGCTGACCTCTGGTGGCCGATCGGGGCACTGCAGGGGCCGAGCCATTTTGGGGGGCCCCCCTC
CTTGCTCTGCAGGCACCTTAGTGGCTTTTTTCCTCCTGTGTACAGGGAAGAGAGGGGTACATTTCCCTGTGCTGACGGAAGCCAACTTGGCTTTCCCGGA
CTGCAAGCAGGGCTCTGCCCCAGAGGCCTCTCTCTCCGTCGTGGGAGAGAGACGTGTACATAGTGTAGGTCAGCGTGCTTAGCCTCCTGACCTGAGGCTC
CTGTGCTACTTTGCCTTTTGCAAACTTTATTTTCATAGATTGAGAAGTTTTGTACAGAGAATTAAAAATGAAATTATTTATAATCTGG
  • refseq_SREBF1.F1 (forward primer)
  • refseq_SREBF1.R1 (reverse primer)
  • Single exon skipping

Frame 0

SRAAAGGTQFPRNFSPAPGRL*GQGRTRTRGAA
AATESRGRGGAP*EGPYEAAGPAGLPEEAAAPWT
SHPSARRLWSRRWASRAIWTRRC*PTSKTCFSL
STTKTVTSLAYLTHPMLGVGQGAQTLPAPIPAP
QAACLHLLPH*APLLKPS*AGRRQRPHPCPLPSL
HPLH*RCTRPCPLSPLGLVSRKSQCH*ASCRPP
PHSPCQGPSCHRASQPQPHRSSAPPLC*ATPAL
REASLQEALPGTPSSRCLACHWLPRQGSRPSPCT
PRSRVWSPSSY*QSQLPPRQPL*RPL*PRRSSR
SRSCCSPTSSRQTRCF*QP*RQTEPL*RRQVSV
PWSLAPLCRQGLCRPW*VAEPSWQQSHWS*MRRS
CLSTGSQLAARPRPLPRAVERSAQPTTPLRSAT
APPSMTKSLSSRIWWWALRQS*INLLSCARPST
TFAFCNTATRNSSRRT*VCALLSTKANL*RIWCR
PVAVEGTQTCSWRA*RLRWRTH*PHPPRMLAHL
SRAAPCPLAAGAVAAVAVAVTRSLTAQSLRTAR
QSQSSGRLCTAGACWTAPAWPCARSSSSACPATP
WPPCWGPGGFPAPQIPPASTIALGATCWAPRAE
MALAGPSGCCPQWSGCSMGCWCSSPWCFSLSTV
SQSHGPTQAPPCTSGGIASRLTWTWPGETLPRLP
SSCGWPCGHWAGPCPPPTWTWLVASSGTSSVTC
CSVSGWAAGWQAGQGACSRTVLCEWMLAPAPET
QPWSTISCTSCTPWGSTQAGTSLPPTWR*VP*TW
QSVQGMPCLWRRWPRSMWRLH*E*RPVSHGPCI
F*HASS*AVPARPAWHRVAQCLLPCSGSATPWA
TVSSWMGTGPCSVPHGRACTAWPGTQWTPWPR*L
SYSGNIS*SEH*TV*PSPTPALGQLMGTRNSRM
PSGTCSC*TAVLMLRGLLPTASPSVPAWPPPPA
*TRWPSGGPL*QLW*STGCGGMRRRLSGCARWWS
TCPGCCRSLRDPCPGQLCTPSRLPGPCWAVPRQ
SLVQPA*PSVRRPVGTCRTAWLPHQPAAPLTRP
CSCSCVTCFLWCAPACGGSSSPRPRPQQPRAPAA
GPRLPPLSCVASNGT*AA*GGWHRASGPPCGGC
SYMRPRPG*WRGPAPHGHTSSSTAV*GGGQAPV
AKEARWRSWSRGPRGGSTRRPCCWPPATCPPASC
RRPGSAWACWLRRRAHSRSLAIAGCCTTVSRCS
CAWAVGPLSLPARPRVPGLSTPVSSHFGPVQLL
SCVEALKAEGSARDSGLHSSTCGCCVPSRWKARG
ARS*P*DRRP*WC*PLVADRGTAGAEPFWGAPL
LALQAP*WLFSSCVQGREGYISLC*RKPTWLSR
TASRALPQRPLSPSWERDVYIV*VSVLSLLT*GS
CATLPFANFIFID*EVLYRELKMKLFIIW

Frame 1

AELRPGEPSFRGTFRRRRAASEARAGHERAERR
RRLRAGAAAALPRKGRTRRRARRASRRRRLRHG
RATLQRGGFGAGAGRAVRSGRGAADRHRRHASAY
QQPRQ*LPWPI*PTLCWEWGRGHRPCQPRYQLP
RQLVSTSCHIELLS*SLPERAAGSALTPVPSPA
CTHSIEDVPVHARFLPWAWYQGRVSATEHPADPH
PTAPARGPPATELPSPSPTAVQLHPCVRLPQPS
GRLLYRKPSREHPAAAAWPATGFPARGPARLLA
HPGPECGPPAATDSHSCPHGSPCNDHCDLADPAG
PGPAAAPLHQGRLAASDSHEDRRSHCEGGRSQS
PGLWHHCADRAFADPGEWRNHLGNSPTGRRCGE
AAYQPARSWQQGPGLCPEPWREAHSPQRH*EALP
LLHQ*QNH*AQGSGGGH*GKAE*ICCLAQGHRL
HSLSATQQPETQAGEPKSAHCCPQKQISEGSGV
GLWQWREHRRAHGGRED*GGGHTDPTPLGCWLTF
PEQPLVPWQQGQWQRWQWQ*LGA*QPSL*GQQG
KARAAAVSAQPGHAGPLPPGPVHARLPLPVLQP
LGLLAGGPGASQPLRYHQRLP*PWAQRAGHREQR
WPWLGPVAAAPSGLAAQWAVGARLLGASLCLR*
ASHTAPLRPRRVLLEASQAG*PGPGPGRLCPGC
PAAVAGPAGTGPAPAHLPPGPGL*PPLEPHPSPA
AASLGGPLAGRPGRGPAAGLCSASGC*RQRPRR
SPGLP*AAPAAHHGEAHRRAPHCHQPGAECPEP
GRVCRGCRVCGDAGRDLCGGCIESEDQSPTGLAF
SDTLLPEQCPPGLPGTEWLSASCHAVALPPRGP
PFLRGWGLVRAQYPMGEPVQLGREPSGPPGPGD
SAIPGTSLRASTELCDPAQPQPWVS*WGQGILGC
PRVPAAAEQLF*CCGGSCLQLLHQFQHGHHHRR
RPGGQVVGLSDSCGDPLAAAG*GGG*AAVPAGG
APAPGAAGV*ETPAQGSSALLQGCPGPAGLCQGR
VWSSQPDHL*EGQWVPAGQPGYHTSQQLH*QGR
AAVPV*PASCGAHQPVAAAAAPGPGPSSPGHQQ
QAPGFRP*AAWLPTGPEQPEAAGTELPARHAEGV
PT*GHGPADGGGQPHTDTPAPRPQSEAAGRPRW
QRRRGGGAGAAAHAAGARGGLAAGLLLPAPRLP
VGARAARGHAG*GGAHTREAWRSPAAARLSADAH
APGRWDHCHFQLDPVSPASAPLSLATLVPCSFC
PASKL*RPKAVQETLASTVRPAAAVCLRGGRPE
GRDLDPKTGGHDGADLWWPIGALQGPSHFGGPPS
LLCRHLSGFFPPVYREERGTFPCADGSQLGFPG
LQAGLCPRGLSLRRGRETCT*CRSACLAS*PEA
PVLLCLLQTLFS*IEKFCTEN*K*NYL*S

Frame 2

QSCGRGNPVSEELFAGAGPPLRPGQDTNARSGG
GD*EPGPRRRSLGRAVRGGGPGGPPGGGGCAMD
EPPFSEAALEQALGEPCDLDAALLTDIEDMLQL
INNQDSDFPGLFDPPYAGSGAGGTDPASPDTSSP
GSLSPPPATLSSSLEAFLSGPQAAPSPLSPPQP
APTPLKMYPSMPAFSPGPGIKEESVPLSILQTP
TPQPLPGALLPQSFPAPAPPQFSSTPVLGYPSPP
GGFSTGSPPGNTQQPLPGLPLASPPGVPPVSLH
TQVQSVVPQQLLTVTAAPTAAPVTTTVTSQIQQ
VPVLLQPHFIKADSLLLTAMKTDGATVKAAGLSP
LVSGTTVQTGPLPTLVSGGTILATVPLVVDAEK
LPINRLAAGSKAPASAQSRGEKRTAHNAIEKRY
RSSINDKIIELKDLVVGTEAKLNKSAVLRKAIDY
IRFLQHSNQKLKQENLSLRTAVHKSKSLKDLVS
ACGSGGNTDVLMEGVKTEVEDTLTPPPSDAGSP
FQSSPLSLGSRGSGSGGSGSDSEPDSPVFEDSKA
KPEQRPSLHSRGMLDRSRLALCTLVFLCLSCNP
LASLLGARGLPSPSDTTSVYHSPGRNVLGTESR
DGPGWAQWLLPPVVWLLNGLLVLVSLVLLFVYGE
PVTRPHSGPAVYFWRHRKQADLDLARGDFAQAA
QQLWLALRALGRPLPTSHLDLACSLLWNLIRHL
LQRLWVGRWLAGRAGGLQQDCALRVDASASARDA
ALVYHKLHQLHTMGKHTGGHLTATNLALSALNL
AECAGDAVSVATLAEIYVAAALRVKTSLPRALH
FLTRFFLSSARQACLAQSGSVPPAMQWLCHPVGH
RFFVDGDWSVLSTPWESLYSLAGNPVDPLAQVT
QLFREHLLERALNCVTQPNPSPGSADGDKEFSD
ALGYLQLLNSCSDAAGAPAYSFSISSSMATTTGV
DPVAKWWASLTAVVIHWLRRDEEAAERLCPLVE
HLPRVLQESERPLPRAALHSFKAARALLGCAKA
ESGPASLTICEKASGYLQDSLATTPASSSIDKAV
QLFLCDLLLVVRTSLWRQQQPPAPAPAAQGTSS
RPQASALELRGFQRDLSSLRRLAQSFRPAMRRV
FLHEATARLMAGASPTRTHQLLDRSLRRRAGPGG
KGGAVAELEPRPTRREHAEALLLASCYLPPGFL
SAPGQRVGMLAEAARTLEKLGDRRLLHDCQQML
MRLGGGTTVTSS*TPCPRPQHPCL*PLWSRAASV
LRRSFEGRRQCKRLWPPQFDLRLLCAFAVEGPR
GAILTLRPAAMMVLTSGGRSGHCRGRAILGGPP
PCSAGTLVAFFLLCTGKRGVHFPVLTEANLAFPD
CKQGSAPEASLSVVGERRVHSVGQRA*PPDLRL
LCYFAFCKLYFHRLRSFVQRIKNEIIYNL

Sequence NM_001005291

AGCAGAGCTGCGGCCGGGGGAACCCAGTTTCCGAGGAACTTTTCGCCGGCGCCGGGCCGCCTCTGAGGCCAGGGCAGGACACGAACGCGCGGAGCGGCGG
CGGCGACTGAGAGCCGGGGCCGCGGCGGCGCTCCCTAGGAAGGGCCGTACGAGGCGGCGGGCCCGGCGGGCCTCCCGGAGGAGGCGGCTGCGCCATGGAC
GAGCCACCCTTCAGCGAGGCGGCTTTGGAGCAGGCGCTGGGCGAGCCGTGCGATCTGGACGCGGCGCTGCTGACCGACATCGAAGGTGAAGTCGGCGCGG
GGAGGGGTAGGGCCAACGGCCTGGACGCCCCAAGGGCGGGCGCAGATCGCGGAGCCATGGATTGCACTTTCGAAGACATGCTTCAGCTTATCAACAACCA
AGACAGTGACTTCCCTGGCCTATTTGACCCACCCTATGCTGGGAGTGGGGCAGGGGGCACAGACCCTGCCAGCCCCGATACCAGCTCCCCAGGCAGCTTG
TCTCCACCTCCTGCCACATTGAGCTCCTCTCTTGAAGCCTTCCTGAGCGGGCCGCAGGCAGCGCCCTCACCCCTGTCCCCTCCCCAGCCTGCACCCACTC
CATTGAAGATGTACCCGTCCATGCCCGCTTTCTCCCCTGGGCCTGGTATCAAGGAAGAGTCAGTGCCACTGAGCATCCTGCAGACCCCCACCCCACAGCC
CCTGCCAGGGGCCCTCCTGCCACAGAGCTTCCCAGCCCCAGCCCCACCGCAGTTCAGCTCCACCCCTGTGTTAGGCTACCCCAGCCCTCCGGGAGGCTTC
TCTACAGGAAGCCCTCCCGGGAACACCCAGCAGCCGCTGCCTGGCCTGCCACTGGCTTCCCCGCCAGGGGTCCCGCCCGTCTCCTTGCACACCCAGGTCC
AGAGTGTGGTCCCCCAGCAGCTACTGACAGTCACAGCTGCCCCCACGGCAGCCCCTGTAACGACCACTGTGACCTCGCAGATCCAGCAGGTCCCGGTCCT
GCTGCAGCCCCACTTCATCAAGGCAGACTCGCTGCTTCTGACAGCCATGAAGACAGACGGAGCCACTGTGAAGGCGGCAGGTCTCAGTCCCCTGGTCTCT
GGCACCACTGTGCAGACAGGGCCTTTGCCGACCCTGGTGAGTGGCGGAACCATCTTGGCAACAGTCCCACTGGTCGTAGATGCGGAGAAGCTGCCTATCA
ACCGGCTCGCAGCTGGCAGCAAGGCCCCGGCCTCTGCCCAGAGCCGTGGAGAGAAGCGCACAGCCCACAACGCCATTGAGAAGCGCTACCGCTCCTCCAT
CAATGACAAAATCATTGAGCTCAAGGATCTGGTGGTGGGCACTGAGGCAAAGCTGAATAAATCTGCTGTCTTGCGCAAGGCCATCGACTACATTCGCTTT
CTGCAACACAGCAACCAGAAACTCAAGCAGGAGAACCTAAGTCTGCGCACTGCTGTCCACAAAAGCAAATCTCTGAAGGATCTGGTGTCGGCCTGTGGCA
GTGGAGGGAACACAGACGTGCTCATGGAGGGCGTGAAGACTGAGGTGGAGGACACACTGACCCCACCCCCCTCGGATGCTGGCTCACCTTTCCAGAGCAG
CCCCTTGTCCCTTGGCAGCAGGGGCAGTGGCAGCGGTGGCAGTGGCAGTGACTCGGAGCCTGACAGCCCAGTCTTTGAGGACAGCAAGGCAAAGCCAGAG
CAGCGGCCGTCTCTGCACAGCCGGGGCATGCTGGACCGCTCCCGCCTGGCCCTGTGCACGCTCGTCTTCCTCTGCCTGTCCTGCAACCCCTTGGCCTCCT
TGCTGGGGGCCCGGGGGCTTCCCAGCCCCTCAGATACCACCAGCGTCTACCATAGCCCTGGGCGCAACGTGCTGGGCACCGAGAGCAGAGATGGCCCTGG
CTGGGCCCAGTGGCTGCTGCCCCCAGTGGTCTGGCTGCTCAATGGGCTGTTGGTGCTCGTCTCCTTGGTGCTTCTCTTTGTCTACGGTGAGCCAGTCACA
CGGCCCCACTCAGGCCCCGCCGTGTACTTCTGGAGGCATCGCAAGCAGGCTGACCTGGACCTGGCCCGGGGAGACTTTGCCCAGGCTGCCCAGCAGCTGT
GGCTGGCCCTGCGGGCACTGGGCCGGCCCCTGCCCACCTCCCACCTGGACCTGGCTTGTAGCCTCCTCTGGAACCTCATCCGTCACCTGCTGCAGCGTCT
CTGGGTGGGCCGCTGGCTGGCAGGCCGGGCAGGGGGCCTGCAGCAGGACTGTGCTCTGCGAGTGGATGCTAGCGCCAGCGCCCGAGACGCAGCCCTGGTC
TACCATAAGCTGCACCAGCTGCACACCATGGGGAAGCACACAGGCGGGCACCTCACTGCCACCAACCTGGCGCTGAGTGCCCTGAACCTGGCAGAGTGTG
CAGGGGATGCCGTGTCTGTGGCGACGCTGGCCGAGATCTATGTGGCGGCTGCATTGAGAGTGAAGACCAGTCTCCCACGGGCCTTGCATTTTCTGACACG
CTTCTTCCTGAGCAGTGCCCGCCAGGCCTGCCTGGCACAGAGTGGCTCAGTGCCTCCTGCCATGCAGTGGCTCTGCCACCCCGTGGGCCACCGTTTCTTC
GTGGATGGGGACTGGTCCGTGCTCAGTACCCCATGGGAGAGCCTGTACAGCTTGGCCGGGAACCCAGTGGACCCCCTGGCCCAGGTGACTCAGCTATTCC
GGGAACATCTCTTAGAGCGAGCACTGAACTGTGTGACCCAGCCCAACCCCAGCCCTGGGTCAGCTGATGGGGACAAGGAATTCTCGGATGCCCTCGGGTA
CCTGCAGCTGCTGAACAGCTGTTCTGATGCTGCGGGGGCTCCTGCCTACAGCTTCTCCATCAGTTCCAGCATGGCCACCACCACCGGCGTAGACCCGGTG
GCCAAGTGGTGGGCCTCTCTGACAGCTGTGGTGATCCACTGGCTGCGGCGGGATGAGGAGGCGGCTGAGCGGCTGTGCCCGCTGGTGGAGCACCTGCCCC
GGGTGCTGCAGGAGTCTGAGAGACCCCTGCCCAGGGCAGCTCTGCACTCCTTCAAGGCTGCCCGGGCCCTGCTGGGCTGTGCCAAGGCAGAGTCTGGTCC
AGCCAGCCTGACCATCTGTGAGAAGGCCAGTGGGTACCTGCAGGACAGCCTGGCTACCACACCAGCCAGCAGCTCCATTGACAAGGCCGTGCAGCTGTTC
CTGTGTGACCTGCTTCTTGTGGTGCGCACCAGCCTGTGGCGGCAGCAGCAGCCCCCGGCCCCGGCCCCAGCAGCCCAGGGCACCAGCAGCAGGCCCCAGG
CTTCCGCCCTTGAGCTGCGTGGCTTCCAACGGGACCTGAGCAGCCTGAGGCGGCTGGCACAGAGCTTCCGGCCCGCCATGCGGAGGGTGTTCCTACATGA
GGCCACGGCCCGGCTGATGGCGGGGGCCAGCCCCACACGGACACACCAGCTCCTCGACCGCAGTCTGAGGCGGCGGGCAGGCCCCGGTGGCAAAGGAGGC
GCGGTGGCGGAGCTGGAGCCGCGGCCCACGCGGCGGGAGCACGCGGAGGCCTTGCTGCTGGCCTCCTGCTACCTGCCCCCCGGCTTCCTGTCGGCGCCCG
GGCAGCGCGTGGGCATGCTGGCTGAGGCGGCGCGCACACTCGAGAAGCTTGGCGATCGCCGGCTGCTGCACGACTGTCAGCAGATGCTCATGCGCCTGGG
CGGTGGGACCACTGTCACTTCCAGCTAGACCCCGTGTCCCCGGCCTCAGCACCCCTGTCTCTAGCCACTTTGGTCCCGTGCAGCTTCTGTCCTGCGTCGA
AGCTTTGAAGGCCGAAGGCAGTGCAAGAGACTCTGGCCTCCACAGTTCGACCTGCGGCTGCTGTGTGCCTTCGCGGTGGAAGGCCCGAGGGGCGCGATCT
TGACCCTAAGACCGGCGGCCATGATGGTGCTGACCTCTGGTGGCCGATCGGGGCACTGCAGGGGCCGAGCCATTTTGGGGGGCCCCCCTCCTTGCTCTGC
AGGCACCTTAGTGGCTTTTTTCCTCCTGTGTACAGGGAAGAGAGGGGTACATTTCCCTGTGCTGACGGAAGCCAACTTGGCTTTCCCGGACTGCAAGCAG
GGCTCTGCCCCAGAGGCCTCTCTCTCCGTCGTGGGAGAGAGACGTGTACATAGTGTAGGTCAGCGTGCTTAGCCTCCTGACCTGAGGCTCCTGTGCTACT
TTGCCTTTTGCAAACTTTATTTTCATAGATTGAGAAGTTTTGTACAGAGAATTAAAAATGAAATTATTTATAATCTGG
  • refseq_SREBF1.F1 (forward primer)
  • refseq_SREBF1.R1 (reverse primer)
  • Single exon skipping

Frame 0

SRAAAGGTQFPRNFSPAPGRL*GQGRTRTRGAA
AATESRGRGGAP*EGPYEAAGPAGLPEEAAAPWT
SHPSARRLWSRRWASRAIWTRRC*PTSKVKSAR
GGVGPTAWTPQGRAQIAEPWIALSKTCFSLSTT
KTVTSLAYLTHPMLGVGQGAQTLPAPIPAPQAAC
LHLLPH*APLLKPS*AGRRQRPHPCPLPSLHPL
H*RCTRPCPLSPLGLVSRKSQCH*ASCRPPPHS
PCQGPSCHRASQPQPHRSSAPPLC*ATPALREAS
LQEALPGTPSSRCLACHWLPRQGSRPSPCTPRS
RVWSPSSY*QSQLPPRQPL*RPL*PRRSSRSRS
CCSPTSSRQTRCF*QP*RQTEPL*RRQVSVPWSL
APLCRQGLCRPW*VAEPSWQQSHWS*MRRSCLS
TGSQLAARPRPLPRAVERSAQPTTPLRSATAPP
SMTKSLSSRIWWWALRQS*INLLSCARPSTTFAF
CNTATRNSSRRT*VCALLSTKANL*RIWCRPVA
VEGTQTCSWRA*RLRWRTH*PHPPRMLAHLSRA
APCPLAAGAVAAVAVAVTRSLTAQSLRTARQSQS
SGRLCTAGACWTAPAWPCARSSSSACPATPWPP
CWGPGGFPAPQIPPASTIALGATCWAPRAEMAL
AGPSGCCPQWSGCSMGCWCSSPWCFSLSTVSQSH
GPTQAPPCTSGGIASRLTWTWPGETLPRLPSSC
GWPCGHWAGPCPPPTWTWLVASSGTSSVTCCSV
SGWAAGWQAGQGACSRTVLCEWMLAPAPETQPWS
TISCTSCTPWGSTQAGTSLPPTWR*VP*TWQSV
QGMPCLWRRWPRSMWRLH*E*RPVSHGPCIF*H
ASS*AVPARPAWHRVAQCLLPCSGSATPWATVSS
WMGTGPCSVPHGRACTAWPGTQWTPWPR*LSYS
GNIS*SEH*TV*PSPTPALGQLMGTRNSRMPSG
TCSC*TAVLMLRGLLPTASPSVPAWPPPPA*TRW
PSGGPL*QLW*STGCGGMRRRLSGCARWWSTCP
GCCRSLRDPCPGQLCTPSRLPGPCWAVPRQSLV
QPA*PSVRRPVGTCRTAWLPHQPAAPLTRPCSCS
CVTCFLWCAPACGGSSSPRPRPQQPRAPAAGPR
LPPLSCVASNGT*AA*GGWHRASGPPCGGCSYM
RPRPG*WRGPAPHGHTSSSTAV*GGGQAPVAKEA
RWRSWSRGPRGGSTRRPCCWPPATCPPASCRRP
GSAWACWLRRRAHSRSLAIAGCCTTVSRCSCAW
AVGPLSLPARPRVPGLSTPVSSHFGPVQLLSCVE
ALKAEGSARDSGLHSSTCGCCVPSRWKARGARS
*P*DRRP*WC*PLVADRGTAGAEPFWGAPLLAL
QAP*WLFSSCVQGREGYISLC*RKPTWLSRTASR
ALPQRPLSPSWERDVYIV*VSVLSLLT*GSCAT
LPFANFIFID*EVLYRELKMKLFIIW

Frame 1

AELRPGEPSFRGTFRRRRAASEARAGHERAERR
RRLRAGAAAALPRKGRTRRRARRASRRRRLRHG
RATLQRGGFGAGAGRAVRSGRGAADRHRR*SRRG
EG*GQRPGRPKGGRRSRSHGLHFRRHASAYQQP
RQ*LPWPI*PTLCWEWGRGHRPCQPRYQLPRQL
VSTSCHIELLS*SLPERAAGSALTPVPSPACTHS
IEDVPVHARFLPWAWYQGRVSATEHPADPHPTA
PARGPPATELPSPSPTAVQLHPCVRLPQPSGRL
LYRKPSREHPAAAAWPATGFPARGPARLLAHPGP
ECGPPAATDSHSCPHGSPCNDHCDLADPAGPGP
AAAPLHQGRLAASDSHEDRRSHCEGGRSQSPGL
WHHCADRAFADPGEWRNHLGNSPTGRRCGEAAYQ
PARSWQQGPGLCPEPWREAHSPQRH*EALPLLH
Q*QNH*AQGSGGGH*GKAE*ICCLAQGHRLHSL
SATQQPETQAGEPKSAHCCPQKQISEGSGVGLWQ
WREHRRAHGGRED*GGGHTDPTPLGCWLTFPEQ
PLVPWQQGQWQRWQWQ*LGA*QPSL*GQQGKAR
AAAVSAQPGHAGPLPPGPVHARLPLPVLQPLGLL
AGGPGASQPLRYHQRLP*PWAQRAGHREQRWPW
LGPVAAAPSGLAAQWAVGARLLGASLCLR*ASH
TAPLRPRRVLLEASQAG*PGPGPGRLCPGCPAAV
AGPAGTGPAPAHLPPGPGL*PPLEPHPSPAAAS
LGGPLAGRPGRGPAAGLCSASGC*RQRPRRSPG
LP*AAPAAHHGEAHRRAPHCHQPGAECPEPGRVC
RGCRVCGDAGRDLCGGCIESEDQSPTGLAFSDT
LLPEQCPPGLPGTEWLSASCHAVALPPRGPPFL
RGWGLVRAQYPMGEPVQLGREPSGPPGPGDSAIP
GTSLRASTELCDPAQPQPWVS*WGQGILGCPRV
PAAAEQLF*CCGGSCLQLLHQFQHGHHHRRRPG
GQVVGLSDSCGDPLAAAG*GGG*AAVPAGGAPAP
GAAGV*ETPAQGSSALLQGCPGPAGLCQGRVWS
SQPDHL*EGQWVPAGQPGYHTSQQLH*QGRAAV
PV*PASCGAHQPVAAAAAPGPGPSSPGHQQQAPG
FRP*AAWLPTGPEQPEAAGTELPARHAEGVPT*
GHGPADGGGQPHTDTPAPRPQSEAAGRPRWQRR
RGGGAGAAAHAAGARGGLAAGLLLPAPRLPVGAR
AARGHAG*GGAHTREAWRSPAAARLSADAHAPG
RWDHCHFQLDPVSPASAPLSLATLVPCSFCPAS
KL*RPKAVQETLASTVRPAAAVCLRGGRPEGRDL
DPKTGGHDGADLWWPIGALQGPSHFGGPPSLLC
RHLSGFFPPVYREERGTFPCADGSQLGFPGLQA
GLCPRGLSLRRGRETCT*CRSACLAS*PEAPVLL
CLLQTLFS*IEKFCTEN*K*NYL*S

Frame 2

QSCGRGNPVSEELFAGAGPPLRPGQDTNARSGG
GD*EPGPRRRSLGRAVRGGGPGGPPGGGGCAMD
EPPFSEAALEQALGEPCDLDAALLTDIEGEVGA
GRGRANGLDAPRAGADRGAMDCTFEDMLQLINNQ
DSDFPGLFDPPYAGSGAGGTDPASPDTSSPGSL
SPPPATLSSSLEAFLSGPQAAPSPLSPPQPAPT
PLKMYPSMPAFSPGPGIKEESVPLSILQTPTPQP
LPGALLPQSFPAPAPPQFSSTPVLGYPSPPGGF
STGSPPGNTQQPLPGLPLASPPGVPPVSLHTQV
QSVVPQQLLTVTAAPTAAPVTTTVTSQIQQVPVL
LQPHFIKADSLLLTAMKTDGATVKAAGLSPLVS
GTTVQTGPLPTLVSGGTILATVPLVVDAEKLPI
NRLAAGSKAPASAQSRGEKRTAHNAIEKRYRSSI
NDKIIELKDLVVGTEAKLNKSAVLRKAIDYIRF
LQHSNQKLKQENLSLRTAVHKSKSLKDLVSACG
SGGNTDVLMEGVKTEVEDTLTPPPSDAGSPFQSS
PLSLGSRGSGSGGSGSDSEPDSPVFEDSKAKPE
QRPSLHSRGMLDRSRLALCTLVFLCLSCNPLAS
LLGARGLPSPSDTTSVYHSPGRNVLGTESRDGPG
WAQWLLPPVVWLLNGLLVLVSLVLLFVYGEPVT
RPHSGPAVYFWRHRKQADLDLARGDFAQAAQQL
WLALRALGRPLPTSHLDLACSLLWNLIRHLLQRL
WVGRWLAGRAGGLQQDCALRVDASASARDAALV
YHKLHQLHTMGKHTGGHLTATNLALSALNLAEC
AGDAVSVATLAEIYVAAALRVKTSLPRALHFLTR
FFLSSARQACLAQSGSVPPAMQWLCHPVGHRFF
VDGDWSVLSTPWESLYSLAGNPVDPLAQVTQLF
REHLLERALNCVTQPNPSPGSADGDKEFSDALGY
LQLLNSCSDAAGAPAYSFSISSSMATTTGVDPV
AKWWASLTAVVIHWLRRDEEAAERLCPLVEHLP
RVLQESERPLPRAALHSFKAARALLGCAKAESGP
ASLTICEKASGYLQDSLATTPASSSIDKAVQLF
LCDLLLVVRTSLWRQQQPPAPAPAAQGTSSRPQ
ASALELRGFQRDLSSLRRLAQSFRPAMRRVFLHE
ATARLMAGASPTRTHQLLDRSLRRRAGPGGKGG
AVAELEPRPTRREHAEALLLASCYLPPGFLSAP
GQRVGMLAEAARTLEKLGDRRLLHDCQQMLMRLG
GGTTVTSS*TPCPRPQHPCL*PLWSRAASVLRR
SFEGRRQCKRLWPPQFDLRLLCAFAVEGPRGAI
LTLRPAAMMVLTSGGRSGHCRGRAILGGPPPCSA
GTLVAFFLLCTGKRGVHFPVLTEANLAFPDCKQ
GSAPEASLSVVGERRVHSVGQRA*PPDLRLLCY
FAFCKLYFHRLRSFVQRIKNEIIYNL

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