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Sequences

Sequence NM_003205

TCCGGAGGCGAGCCGAGCGCGGTGGTGAGGCCGCCTCAGCGAAAAAAATGTCCGCCTGAAGAGACCCACAAGTTCTATTCGGGGGGACCGACAGCCCGCC
CCGGGAGGAAGGGGCGGCCAGGCCCGAAAGCCGCCTCCCCCTCCCAGACCCGAGAGCTCGTGCGGGGCAAAGTGAACCGAGCCGCTGGGCGGTGCAAGGG
GAAGCCCAAGCCCGTTCTCCCGGCCAAAGTGAACTTTAATCGGGGTGGTTGGATGCGGAGACGGGGCGGCAGGACCTGCTAGAAGTGGCCGAAGATGAAT
CCCCAGCAACAACGCATGGCCGCTATAGGGACCGACAAGGAGCTGAGCGACCTACTGGACTTCAGTGCGATGTTTTCCCCACCTGTTAATAGTGGGAAAA
CTAGACCAACTACACTGGGAAGCAGTCAATTCAGTGGATCAGGTATTGATGAAAGAGGAGGTACAACATCTTGGGGAACAAGTGGTCAACCAAGTCCTTC
CTATGATTCATCTAGAGGTTTTACAGACAGCCCTCATTACAGTGATCACTTGAATGACAGTCGATTAGGAGCCCATGAAGGCTTGTCCCCAACACCTTTC
ATGAACTCAAATCTGATGGGAAAAACATCAGAGAGAGGCTCATTTTCCCTGTACAGCAGAGATACTGGATTACCAGGCTGTCAATCTAGTCTCCTGAGAC
AAGATCTGGGGCTTGGGAGCCCAGCACAGCTATCTTCTTCAGGAAAACCTGGGACAGCATACTATTCATTCTCTGCTACAAGTTCCAGGAGGAGACCACT
CCATGACTCTGCAGCGCTTGATCCCTTGCAAGCAAAAAAAGTCAGAAAGGTGCCTCCTGGTTTGCCTTCTTCTGTATATGCACCATCCCCAAATTCAGAT
GATTTCAACCGTGAATCTCCTAGTTATCCATCTCCTAAGCCACCAACCAGTATGTTCGCTAGCACTTTCTTTATGCAAGATGGGACCCACAATTCTTCTG
ACCTTTGGAGTTCATCAAATGGGATGAGCCAGCCTGGTTTTGGTGGAATTCTGGGGACCTCCACTTCCCACATGTCTCAATCCAGTAGTTATGGCAACCT
TCATTCACATGACCGCTTGAGTTATCCTCCACACTCAGTTTCACCAACAGACATAAACACGAGTCTTCCACCAATGTCCAGCTTTCATCGCGGCAGTACC
AGCAGTTCACCTTACGTTGCTGCCTCACACACTCCTCCCATCAATGGATCAGACAGCATTCTAGGAACCAGAGGGAATGCTGCTGGAAGCTCACAGACAG
GTGATGCACTTGGAAAGGCTTTGGCATCTATTTATTCTCCTGACCATACCAGCAGTAGTTTTCCGTCAAATCCATCAACACCAGTTGGATCACCTTCACC
TCTCACAGGTACCAGTCAGTGGCCAAGACCTGGAGGGCAAGCACCTTCATCCCCAAGCTATGAAAACTCACTCCACTCCCTGCAGTCTCGAATGGAGGAT
CGTTTAGACAGACTGGATGATGCAATCCATGTGCTGCGGAACCATGCTGTGGGACCTTCCACCAGTTTGCCTGCTGGTCACAGTGATATACATAGTTTAT
TGGGACCATCCCATAATGCACCAATTGGAAGCCTCAATTCAAACTATGGAGGATCAAGCCTTGTTGCAAGCAGTCGATCAGCTTCAATGGTTGGAACTCA
TCGGGAAGACTCTGTCAGTCTCAATGGCAATCATTCAGTCCTGTCTAGTACAGTCACTACTTCAAGCACAGACCTGAACCATAAAACACAAGAAAATTAT
AGAGGTGGCTTGCAAAGTCAGTCTGGAACTGTTGTTACAACAGAAATCAAGACTGAAAACAAAGAAAAGGATGAAAACCTTCATGAACCTCCTTCATCAG
ATGACATGAAGTCAGATGATGAATCCTCCCAAAAAGATATCAAGGTTTCATCTAGAGGCAGAACAAGCAGTACTAATGAAGATGAGGATTTGAACCCTGA
ACAGAAGATAGAAAGGGAGAAGGAGAGGCGGATGGCTAACAATGCCAGAGAACGCTTACGCGTGCGGGATATTAATGAAGCATTCAAAGAGCTTGGCCGA
ATGTGTCAGCTTCACTTGAAGAGTGAAAAACCCCAAACAAAACTCCTTATTCTTCATCAAGCCGTGGCAGTCATCCTTAGTCTAGAACAGCAAGTCAGAG
AGAGGAACCTTAACCCCAAAGCAGCCTGCCTTAAGAGAAGGGAAGAAGAAAAAGTTTCTGCCGTATCGGCAGAGCCGCCAACCACACTGCCAGGAACCCA
TCCTGGGCTTAGTGAAACTACCAACCCTATGGGTCATATGTAAACATCAGCCAGTTCCAGAGTTATCAGTAGGCTAGATAGAAGGTGACCTCTCCTCATA
AGGACTTGGACAACTCAGATTATCTGAAGACACAAACCTGACAGGAGGGAGAAGAAAAAACAAAACACTTGAACCAAGAAACTCAAATGTAATCCTACGA
TCAAAGCAACTGGTCAACACTTCCATCAGAAGTGAAGATAGGAAGCTCATCAGATAGAACATCAGCCCATGAGATGTTTGCAACAAATCTTTTGTTGCAA
GCAGTGTGTCGCTTCTGCACAATCAGAGACTGTCTCGATCTCTCCACTCACCGTGGAAGTTGCCTTGTGCCTAAACTGAATTGACAAATGCATTGTAACT
ACAAATTTTATTTATTGTTATGAAACTGTAAGGTCTACATATAAAGGGAAAAAGTTAATGTGGAAAGCTGATCTACACTCAGCTGATGCCAGCATACATT
AAAGCGGTTCACGTGCAGAGAACAAAGCAGTGACAACCATTGGCCCTTAGCATTCCCGGCATACCTATTAGTGTCTTAAAAAGGAAGGGAAAAGTCTTTT
GTTGCCCTCTCCTATCCTCTTGCCATATGAATAGCGTTTTCCATGAAATAGGAAAATATTACTTGGTATAGCATTTCTCTTGCTCTCATTTTTTGATTTA
TTTTTATTTTCTCTTTGTGGGTGTTATATTTGATCTCTAAATCTGAACAGTTTATGGTCACAGTCCAGCCTCCTCCGTGCAGCCCTGTGTGCTTTGCACA
TTTACCTTACAGTGGTAAGCAGAGACCATCTGTGACCATAGCCTAGCTAGCATTTTAAAAGGGGAAATTTTGTTCTCTAGGTTTTCCCCCAAATAAACAT
TGCTTTATTTCTAATAATAACCAAGACTTTTCAAGCTTCTAGATCTCATAGGAAAGCTTGTAATAGCAAAATTGTAAATTACAAGGGAAGAATCTACTTT
TTAGAAATCGCTTTGTTTTCCAAGCAGTAAGTACTACATACAGTACTTGTAAAGTGTTAGCTGTAAGTAAGCACAAAATACATTTAAAATACAAAGACGA
TTTTTTCAGGCTGTGATTATGGTGAACATAACAAAACCCAGTAGTCACCAAGGCAGGTAGTGTGATAAATGAACACACCACTCTGAGGCTAATTACCTAA
TGGAATACAAGAGCAATGGTCACCCGTATTTCCTTATCCTAGCCTTTATTTCTCTGTCATTTGGATGGCTGGTCAATGGGGAAGAATTGAGTGGGTGATT
TAATCAACTGCAAACCATCTGCCCCTGTCCCAAAATGATGAGCCAGATTAGCATTAAACCAGTACTTGTCAGTCCATCTTAATACTGTTCATTAAGGCAC
TCTCTGTCTCTAATCCTTAGGAGTTGTTTTAAAAGACATAATCACTTTGAACTTCCATGAAACCTGTCTTCCACCACAACAACCCTGGGAGAGAAAAACA
TGCTAAAGGAGGTATCTTGGCTTAATAATTCCTTATAGCCAATATCAACAGTGGCAATCAGCACACAGAGGAAAGGACCCAAATCACTATGTAGCTTAAA
GATTTCTGTTAATTTGAAAGAACAAAAACAAGACAGAACTTCTGGTACTCTAATCAGGATGATTCCTAACAAGTCAGTCATTTGTGAACTTAGTGGACTT
TTTGGTTACTTTAATTTGCATATATTCTCCAGTTACATCGGACTCTATCTGTGGCCTTGTTCTTCATTTCAGTGTTAATCAGCTAAACAGAAGTTGTTGC
TTATGATGTGTGAGTGAACATATGCCACTGCCTGGCCTTTTTTTCTTCAGAGCTTGTTGTCTTTTTCGCTATATTAGACTTTGCAGTATGCCCAGAAGCT
TTCCTTCATAAAATAGAAAGAAAAAAACATTTGGCTTATTTTTCACTGTAGCTAGTCTTTTATACAATAATCTTGTAAGAAAATTTCTTGAATTCTAAAT
ATTACTCTTTCTAGATTTTTGAAATCAAAAAGTTTTCAGTAAAAAGTTTCTTACTTTATTTTATTATATTAGGTAGTAAAAAATGTAGGGTTATTTACCA
TAACCTGTTCATTAATATCAGAAATTTACAATAGCATTTTAAGACCATAGTAGGATTCTAGCATACCGTGTAGTACCTATGGAGTATTGTAAGAGCTAAT
TGTTGGAGATGAATTGCTTCTCATCTTGTTCTCCAGTTTCCATTGTTGGTTTATTGCAGATTTGTATCCTGTGTCAAATTCAAGGTATTATTGATAAACC
TTTTCAACCAGCAGCAAGAAGTTCAAATTTTTTTCTGTCACTGTAACAGAAAACACAATATGTATATAACATTTATGTAGCAATAAATGTGCCATCTTTT
TTTTAACACAGTAAAA
  • refseq_TCF12.F2 (forward primer)
  • refseq_TCF12.R2 (reverse primer)
  • Single exon skipping

Frame 0

SGGEPSAVVRPPQRKKCPPEETHKFYSGGPTAR
PGRKGRPGPKAASPSQTRELVRGKVNRAAGRCKG
KPKPVLPAKVNFNRGGWMRRRGGRTC*KWPKMN
PQQQRMAAIGTDKELSDLLDFSAMFSPPVNSGK
TRPTTLGSSQFSGSGIDERGGTTSWGTSGQPSPS
YDSSRGFTDSPHYSDHLNDSRLGAHEGLSPTPF
MNSNLMGKTSERGSFSLYSRDTGLPGCQSSLLR
QDLGLGSPAQLSSSGKPGTAYYSFSATSSRRRPL
HDSAALDPLQAKKVRKVPPGLPSSVYAPSPNSD
DFNRESPSYPSPKPPTSMFASTFFMQDGTHNSS
DLWSSSNGMSQPGFGGILGTSTSHMSQSSSYGNL
HSHDRLSYPPHSVSPTDINTSLPPMSSFHRGST
SSSPYVAASHTPPINGSDSILGTRGNAAGSSQT
GDALGKALASIYSPDHTSSSFPSNPSTPVGSPSP
LTGTSQWPRPGGQAPSSPSYENSLHSLQSRMED
RLDRLDDAIHVLRNHAVGPSTSLPAGHSDIHSL
LGPSHNAPIGSLNSNYGGSSLVASSRSASMVGTH
REDSVSLNGNHSVLSSTVTTSSTDLNHKTQENY
RGGLQSQSGTVVTTEIKTENKEKDENLHEPPSS
DDMKSDDESSQKDIKVSSRGRTSSTNEDEDLNPE
QKIEREKERRMANNARERLRVRDINEAFKELGR
MCQLHLKSEKPQTKLLILHQAVAVILSLEQQVR
ERNLNPKAACLKRREEEKVSAVSAEPPTTLPGTH
PGLSETTNPMGHM*TSASSRVISRLDRR*PLLI
RTWTTQII*RHKPDRREKKKQNT*TKKLKCNPT
IKATGQHFHQK*R*EAHQIEHQPMRCLQQIFCCK
QCVASAQSETVSISPLTVEVALCLN*IDKCIVT
TNFIYCYETVRSTYKGKKLMWKADLHSADASIH
*SGSRAENKAVTTIGP*HSRHTY*CLKKEGKSLL
LPSPILLPYE*RFP*NRKILLGIAFLLLSFFDL
FLFSLCGCYI*SLNLNSLWSQSSLLRAALCALH
IYLTVVSRDHL*P*PS*HFKRGNFVL*VFPQINI
ALFLIITKTFQASRSHRKACNSKIVNYKGRIYF
LEIALFSKQ*VLHTVLVKC*L*VSTKYI*NTKT
IFSGCDYGEHNKTQ*SPRQVV**MNTPL*G*LPN
GIQEQWSPVFPYPSLYFSVIWMAGQWGRIEWVI
*STANHLPLSQNDEPD*H*TSTCQSILILFIKA
LSVSNP*ELF*KT*SL*TSMKPVFHHNNPGREKH
AKGGILA**FLIANINSGNQHTEERTQITM*LK
DFC*FERTKTRQNFWYSNQDDS*QVSHL*T*WT
FWLL*FAYILQLHRTLSVALFFISVLIS*TEVVA
YDV*VNICHCLAFFSSELVVFFAILDFAVCPEA
FLHKIERKKHLAYFSL*LVFYTIIL*ENFLNSK
YYSF*IFEIKKFSVKSFLLYFIILGSKKCRVIYH
NLFINIRNLQ*HFKTIVGF*HTV*YLWSIVRAN
CWR*IASHLVLQFPLLVYCRFVSCVKFKVLLIN
LFNQQQEVQIFFCHCNRKHNMYITFM*Q*MCHLF
FNTVK

Frame 1

PEASRARW*GRLSEKNVRLKRPTSSIRGDRQPA
PGGRGGQARKPPPPPRPESSCGAK*TEPLGGAR
GSPSPFSRPK*TLIGVVGCGDGAAGPARSGRR*I
PSNNAWPL*GPTRS*ATYWTSVRCFPHLLIVGK
LDQLHWEAVNSVDQVLMKEEVQHLGEQVVNQVL
PMIHLEVLQTALITVIT*MTVD*EPMKACPQHLS
*TQI*WEKHQREAHFPCTAEILDYQAVNLVS*D
KIWGLGAQHSYLLQENLGQHTIHSLLQVPGGDH
SMTLQRLIPCKQKKSERCLLVCLLLYMHHPQIQM
ISTVNLLVIHLLSHQPVCSLALSLCKMGPTILL
TFGVHQMG*ASLVLVEFWGPPLPTCLNPVVMAT
FIHMTA*VILHTQFHQQT*TRVFHQCPAFIAAVP
AVHLTLLPHTLLPSMDQTAF*EPEGMLLEAHRQ
VMHLERLWHLFILLTIPAVVFRQIHQHQLDHLH
LSQVPVSGQDLEGKHLHPQAMKTHSTPCSLEWRI
V*TDWMMQSMCCGTMLWDLPPVCLLVTVIYIVY
WDHPIMHQLEASIQTMEDQALLQAVDQLQWLEL
IGKTLSVSMAIIQSCLVQSLLQAQT*TIKHKKII
EVACKVSLELLLQQKSRLKTKKRMKTFMNLLHQ
MT*SQMMNPPKKISRFHLEAEQAVLMKMRI*TL
NRR*KGRRRGGWLTMPENAYACGILMKHSKSLAE
CVSFT*RVKNPKQNSLFFIKPWQSSLV*NSKSE
RGTLTPKQPALREGKKKKFLPYRQSRQPHCQEP
ILGLVKLPTLWVICKHQPVPELSVG*IEGDLSS*
GLGQLRLSEDTNLTGGRRKNKTLEPRNSNVILR
SKQLVNTSIRSEDRKLIR*NISP*DVCNKSFVA
SSVSLLHNQRLSRSLHSPWKLPCA*TELTNAL*L
QILFIVMKL*GLHIKGKS*CGKLIYTQLMPAYI
KAVHVQRTKQ*QPLALSIPGIPISVLKRKGKVF
CCPLLSSCHMNSVFHEIGKYYLV*HFSCSHFLIY
FYFLFVGVIFDL*I*TVYGHSPASSVQPCVLCT
FTLQW*AETICDHSLASILKGEILFSRFSPK*T
LLYF***PRLFKLLDLIGKLVIAKL*ITREESTF
*KSLCFPSSKYYIQYL*SVSCK*AQNTFKIQRR
FFQAVIMVNITKPSSHQGR*CDK*THHSEANYL
MEYKSNGHPYFLILAFISLSFGWLVNGEELSG*F
NQLQTICPCPKMMSQISIKPVLVSPS*YCSLRH
SLSLILRSCFKRHNHFELP*NLSSTTTTLGEKN
MLKEVSWLNNSL*PISTVAISTQRKGPKSLCSLK
ISVNLKEQKQDRTSGTLIRMIPNKSVICELSGL
FGYFNLHIFSSYIGLYLWPCSSFQC*SAKQKLL
LMMCE*TYATAWPFFLQSLLSFSLY*TLQYAQKL
SFIK*KEKNIWLIFHCS*SFIQ*SCKKIS*ILN
ITLSRFLKSKSFQ*KVSYFILLY*VVKNVGLFT
ITCSLISEIYNSILRP**DSSIPCSTYGVL*ELI
VGDELLLILFSSFHCWFIADLYPVSNSRYY**T
FSTSSKKFKFFSVTVTENTICI*HLCSNKCAIF
FLTQ*

Frame 2

RRRAERGGEAASAKKMSA*RDPQVLFGGTDSPP
REEGAARPESRLPLPDPRARAGQSEPSRWAVQG
EAQARSPGQSEL*SGWLDAETGRQDLLEVAEDE
SPATTHGRYRDRQGAERPTGLQCDVFPTC**WEN
*TNYTGKQSIQWIRY**KRRYNILGNKWSTKSF
L*FI*RFYRQPSLQ*SLE*QSIRSP*RLVPNTF
HELKSDGKNIRERLIFPVQQRYWITRLSI*SPET
RSGAWEPSTAIFFRKTWDSILFILCYKFQEETT
P*LCSA*SLASKKSQKGASWFAFFCICTIPKFR
*FQP*IS*LSIS*ATNQYVR*HFLYARWDPQFF*
PLEFIKWDEPAWFWWNSGDLHFPHVSIQ*LWQP
SFT*PLELSSTLSFTNRHKHESSTNVQLSSRQY
QQFTLRCCLTHSSHQWIRQHSRNQRECCWKLTDR
*CTWKGFGIYLFS*PYQQ*FSVKSINTSWITFT
SHRYQSVAKTWRASTFIPKL*KLTPLPAVSNGG
SFRQTG*CNPCAAEPCCGTFHQFACWSQ*YT*FI
GTIP*CTNWKPQFKLWRIKPCCKQSISFNGWNS
SGRLCQSQWQSFSPV*YSHYFKHRPEP*NTRKL
*RWLAKSVWNCCYNRNQD*KQRKG*KPS*TSFIR
*HEVR**ILPKRYQGFI*RQNKQY**R*GFEP*
TEDRKGEGEADG*QCQRTLTRAGY**SIQRAWP
NVSASLEE*KTPNKTPYSSSSRGSHP*SRTASQR
EEP*PQSSLP*EKGRRKSFCRIGRAANHTARNP
SWA**NYQPYGSYVNISQFQSYQ*AR*KVTSPH
KDLDNSDYLKTQT*QEGEEKTKHLNQETQM*SYD
QSNWSTLPSEVKIGSSSDRTSAHEMFATNLLLQ
AVCRFCTIRDCLDLSTHRGSCLVPKLN*QMHCN
YKFYLLL*NCKVYI*REKVNVES*STLS*CQHTL
KRFTCREQSSDNHWPLAFPAYLLVS*KGREKSF
VALSYPLAI*IAFSMK*ENITWYSISLALIF*F
IFIFSLWVLYLISKSEQFMVTVQPPPCSPVCFAH
LPYSGKQRPSVTIA*LAF*KGKFCSLGFPPNKH
CFISNNNQDFSSF*IS*ESL**QNCKLQGKNLL
FRNRFVFQAVSTTYSTCKVLAVSKHKIHLKYKDD
FFRL*LW*T*QNPVVTKAGSVINEHTTLRLIT*
WNTRAMVTRISLS*PLFLCHLDGWSMGKN*VGD
LINCKPSAPVPK**ARLALNQYLSVHLNTVH*GT
LCL*SLGVVLKDIITLNFHETCLPPQQPWERKT
C*RRYLGLIIPYSQYQQWQSAHRGKDPNHYVA*
RFLLI*KNKNKTELLVL*SG*FLTSQSFVNLVDF
LVTLICIYSPVTSDSICGLVLHFSVNQLNRSCC
L*CVSEHMPLPGLFFFRACCLFRYIRLCSMPRS
FPS*NRKKKTFGLFFTVASLLYNNLVRKFLEF*I
LLFLDF*NQKVFSKKFLTLFYYIR**KM*GYLP
*PVH*YQKFTIAF*DHSRILAYRVVPMEYCKS*
LLEMNCFSSCSPVSIVGLLQICILCQIQGIIDKP
FQPAARSSNFFLSL*QKTQYVYNIYVAINVPSF
F*HSK

Sequence NM_207038

TCCGGAGGCGAGCCGAGCGCGGTGGTGAGGCCGCCTCAGCGAAAAAAATGTCCGCCTGAAGAGACCCACAAGTTCTATTCGGGGGGACCGACAGCCCGCC
CCGGGAGGAAGGGGCGGCCAGGCCCGAAAGCCGCCTCCCCCTCCCAGACCCGAGAGCTCGTGCGGGGCAAAGTGAACCGAGCCGCTGGGCGGTGCAAGGG
GAAGCCCAAGCCCGTTCTCCCGGCCAAAGTGAACTTTAATCGGGGTGGTTGGATGCGGAGACGGGGCGGCAGAAGTGGCCGAAGATGAATCCCCAGCAAC
AACGCATGGCCGCTATAGGGACCGACAAGGAGCTGAGCGACCTACTGGACTTCAGTGCGATGTTTTCCCCACCTGTTAATAGTGGGAAAACTAGACCAAC
TACACTGGGAAGCAGTCAATTCAGTGGATCAGGTATTGATGAAAGAGGAGGTACAACATCTTGGGGAACAAGTGGTCAACCAAGTCCTTCCTATGATTCA
TCTAGAGGTTTTACAGACAGCCCTCATTACAGTGATCACTTGAATGACAGTCGATTAGGAGCCCATGAAGGCTTGTCCCCAACACCTTTCATGAACTCAA
ATCTGATGGGAAAAACATCAGAGAGAGGCTCATTTTCCCTGTACAGCAGAGATACTGGATTACCAGGCTGTCAATCTAGTCTCCTGAGACAAGATCTGGG
GCTTGGGAGCCCAGCACAGCTATCTTCTTCAGGAAAACCTGGGACAGCATACTATTCATTCTCTGCTACAAGTTCCAGGAGGAGACCACTCCATGACTCT
GCAGCGCTTGATCCCTTGCAAGCAAAAAAAGTCAGAAAGGTGCCTCCTGGTTTGCCTTCTTCTGTATATGCACCATCCCCAAATTCAGATGATTTCAACC
GTGAATCTCCTAGTTATCCATCTCCTAAGCCACCAACCAGTATGTTCGCTAGCACTTTCTTTATGCAAGATGGGACCCACAATTCTTCTGACCTTTGGAG
TTCATCAAATGGGATGAGCCAGCCTGGTTTTGGTGGAATTCTGGGGACCTCCACTTCCCACATGTCTCAATCCAGTAGTTATGGCAACCTTCATTCACAT
GACCGCTTGAGTTATCCTCCACACTCAGTTTCACCAACAGACATAAACACGAGTCTTCCACCAATGTCCAGCTTTCATCGCGGCAGTACCAGCAGTTCAC
CTTACGTTGCTGCCTCACACACTCCTCCCATCAATGGATCAGACAGCATTCTAGGAACCAGAGGGAATGCTGCTGGAAGCTCACAGACAGGTGATGCACT
TGGAAAGGCTTTGGCATCTATTTATTCTCCTGACCATACCAGCAGTAGTTTTCCGTCAAATCCATCAACACCAGTTGGATCACCTTCACCTCTCACAGGT
ACCAGTCAGTGGCCAAGACCTGGAGGGCAAGCACCTTCATCCCCAAGCTATGAAAACTCACTCCACTCCCTGCAGTCTCGAATGGAGGATCGTTTAGACA
GACTGGATGATGCAATCCATGTGCTGCGGAACCATGCTGTGGGACCTTCCACCAGTTTGCCTGCTGGTCACAGTGATATACATAGTTTATTGGGACCATC
CCATAATGCACCAATTGGAAGCCTCAATTCAAACTATGGAGGATCAAGCCTTGTTGCAAGCAGTCGATCAGCTTCAATGGTTGGAACTCATCGGGAAGAC
TCTGTCAGTCTCAATGGCAATCATTCAGTCCTGTCTAGTACAGTCACTACTTCAAGCACAGACCTGAACCATAAAACACAAGAAAATTATAGAGGTGGCT
TGCAAAGTCAGTCTGGAACTGTTGTTACAACAGAAATCAAGACTGAAAACAAAGAAAAGGATGAAAACCTTCATGAACCTCCTTCATCAGATGACATGAA
GTCAGATGATGAATCCTCCCAAAAAGATATCAAGGTTTCATCTAGAGGCAGAACAAGCAGTACTAATGAAGATGAGGATTTGAACCCTGAACAGAAGATA
GAAAGGGAGAAGGAGAGGCGGATGGCTAACAATGCCAGAGAACGCTTACGCGTGCGGGATATTAATGAAGCATTCAAAGAGCTTGGCCGAATGTGTCAGC
TTCACTTGAAGAGTGAAAAACCCCAAACAAAACTCCTTATTCTTCATCAAGCCGTGGCAGTCATCCTTAGTCTAGAACAGCAAGTCAGAGAGAGGAACCT
TAACCCCAAAGCAGCCTGCCTTAAGAGAAGGGAAGAAGAAAAAGTTTCTGCCGTATCGGCAGAGCCGCCAACCACACTGCCAGGAACCCATCCTGGGCTT
AGTGAAACTACCAACCCTATGGGTCATATGTAAACATCAGCCAGTTCCAGAGTTATCAGTAGGCTAGATAGAAGGTGACCTCTCCTCATAAGGACTTGGA
CAACTCAGATTATCTGAAGACACAAACCTGACAGGAGGGAGAAGAAAAAACAAAACACTTGAACCAAGAAACTCAAATGTAATCCTACGATCAAAGCAAC
TGGTCAACACTTCCATCAGAAGTGAAGATAGGAAGCTCATCAGATAGAACATCAGCCCATGAGATGTTTGCAACAAATCTTTTGTTGCAAGCAGTGTGTC
GCTTCTGCACAATCAGAGACTGTCTCGATCTCTCCACTCACCGTGGAAGTTGCCTTGTGCCTAAACTGAATTGACAAATGCATTGTAACTACAAATTTTA
TTTATTGTTATGAAACTGTAAGGTCTACATATAAAGGGAAAAAGTTAATGTGGAAAGCTGATCTACACTCAGCTGATGCCAGCATACATTAAAGCGGTTC
ACGTGCAGAGAACAAAGCAGTGACAACCATTGGCCCTTAGCATTCCCGGCATACCTATTAGTGTCTTAAAAAGGAAGGGAAAAGTCTTTTGTTGCCCTCT
CCTATCCTCTTGCCATATGAATAGCGTTTTCCATGAAATAGGAAAATATTACTTGGTATAGCATTTCTCTTGCTCTCATTTTTTGATTTATTTTTATTTT
CTCTTTGTGGGTGTTATATTTGATCTCTAAATCTGAACAGTTTATGGTCACAGTCCAGCCTCCTCCGTGCAGCCCTGTGTGCTTTGCACATTTACCTTAC
AGTGGTAAGCAGAGACCATCTGTGACCATAGCCTAGCTAGCATTTTAAAAGGGGAAATTTTGTTCTCTAGGTTTTCCCCCAAATAAACATTGCTTTATTT
CTAATAATAACCAAGACTTTTCAAGCTTCTAGATCTCATAGGAAAGCTTGTAATAGCAAAATTGTAAATTACAAGGGAAGAATCTACTTTTTAGAAATCG
CTTTGTTTTCCAAGCAGTAAGTACTACATACAGTACTTGTAAAGTGTTAGCTGTAAGTAAGCACAAAATACATTTAAAATACAAAGACGATTTTTTCAGG
CTGTGATTATGGTGAACATAACAAAACCCAGTAGTCACCAAGGCAGGTAGTGTGATAAATGAACACACCACTCTGAGGCTAATTACCTAATGGAATACAA
GAGCAATGGTCACCCGTATTTCCTTATCCTAGCCTTTATTTCTCTGTCATTTGGATGGCTGGTCAATGGGGAAGAATTGAGTGGGTGATTTAATCAACTG
CAAACCATCTGCCCCTGTCCCAAAATGATGAGCCAGATTAGCATTAAACCAGTACTTGTCAGTCCATCTTAATACTGTTCATTAAGGCACTCTCTGTCTC
TAATCCTTAGGAGTTGTTTTAAAAGACATAATCACTTTGAACTTCCATGAAACCTGTCTTCCACCACAACAACCCTGGGAGAGAAAAACATGCTAAAGGA
GGTATCTTGGCTTAATAATTCCTTATAGCCAATATCAACAGTGGCAATCAGCACACAGAGGAAAGGACCCAAATCACTATGTAGCTTAAAGATTTCTGTT
AATTTGAAAGAACAAAAACAAGACAGAACTTCTGGTACTCTAATCAGGATGATTCCTAACAAGTCAGTCATTTGTGAACTTAGTGGACTTTTTGGTTACT
TTAATTTGCATATATTCTCCAGTTACATCGGACTCTATCTGTGGCCTTGTTCTTCATTTCAGTGTTAATCAGCTAAACAGAAGTTGTTGCTTATGATGTG
TGAGTGAACATATGCCACTGCCTGGCCTTTTTTTCTTCAGAGCTTGTTGTCTTTTTCGCTATATTAGACTTTGCAGTATGCCCAGAAGCTTTCCTTCATA
AAATAGAAAGAAAAAAACATTTGGCTTATTTTTCACTGTAGCTAGTCTTTTATACAATAATCTTGTAAGAAAATTTCTTGAATTCTAAATATTACTCTTT
CTAGATTTTTGAAATCAAAAAGTTTTCAGTAAAAAGTTTCTTACTTTATTTTATTATATTAGGTAGTAAAAAATGTAGGGTTATTTACCATAACCTGTTC
ATTAATATCAGAAATTTACAATAGCATTTTAAGACCATAGTAGGATTCTAGCATACCGTGTAGTACCTATGGAGTATTGTAAGAGCTAATTGTTGGAGAT
GAATTGCTTCTCATCTTGTTCTCCAGTTTCCATTGTTGGTTTATTGCAGATTTGTATCCTGTGTCAAATTCAAGGTATTATTGATAAACCTTTTCAACCA
GCAGCAAGAAGTTCAAATTTTTTTCTGTCACTGTAACAGAAAACACAATATGTATATAACATTTATGTAGCAATAAATGTGCCATCTTTTTTTTAACACA
GTAAAA
  • refseq_TCF12.F2 (forward primer)
  • refseq_TCF12.R2 (reverse primer)
  • Single exon skipping

Frame 0

SGGEPSAVVRPPQRKKCPPEETHKFYSGGPTAR
PGRKGRPGPKAASPSQTRELVRGKVNRAAGRCKG
KPKPVLPAKVNFNRGGWMRRRGGRSGRR*IPSN
NAWPL*GPTRS*ATYWTSVRCFPHLLIVGKLDQ
LHWEAVNSVDQVLMKEEVQHLGEQVVNQVLPMIH
LEVLQTALITVIT*MTVD*EPMKACPQHLS*TQ
I*WEKHQREAHFPCTAEILDYQAVNLVS*DKIW
GLGAQHSYLLQENLGQHTIHSLLQVPGGDHSMTL
QRLIPCKQKKSERCLLVCLLLYMHHPQIQMIST
VNLLVIHLLSHQPVCSLALSLCKMGPTILLTFG
VHQMG*ASLVLVEFWGPPLPTCLNPVVMATFIHM
TA*VILHTQFHQQT*TRVFHQCPAFIAAVPAVH
LTLLPHTLLPSMDQTAF*EPEGMLLEAHRQVMH
LERLWHLFILLTIPAVVFRQIHQHQLDHLHLSQV
PVSGQDLEGKHLHPQAMKTHSTPCSLEWRIV*T
DWMMQSMCCGTMLWDLPPVCLLVTVIYIVYWDH
PIMHQLEASIQTMEDQALLQAVDQLQWLELIGKT
LSVSMAIIQSCLVQSLLQAQT*TIKHKKIIEVA
CKVSLELLLQQKSRLKTKKRMKTFMNLLHQMT*
SQMMNPPKKISRFHLEAEQAVLMKMRI*TLNRR*
KGRRRGGWLTMPENAYACGILMKHSKSLAECVS
FT*RVKNPKQNSLFFIKPWQSSLV*NSKSERGT
LTPKQPALREGKKKKFLPYRQSRQPHCQEPILGL
VKLPTLWVICKHQPVPELSVG*IEGDLSS*GLG
QLRLSEDTNLTGGRRKNKTLEPRNSNVILRSKQ
LVNTSIRSEDRKLIR*NISP*DVCNKSFVASSVS
LLHNQRLSRSLHSPWKLPCA*TELTNAL*LQIL
FIVMKL*GLHIKGKS*CGKLIYTQLMPAYIKAV
HVQRTKQ*QPLALSIPGIPISVLKRKGKVFCCPL
LSSCHMNSVFHEIGKYYLV*HFSCSHFLIYFYF
LFVGVIFDL*I*TVYGHSPASSVQPCVLCTFTL
QW*AETICDHSLASILKGEILFSRFSPK*TLLYF
***PRLFKLLDLIGKLVIAKL*ITREESTF*KS
LCFPSSKYYIQYL*SVSCK*AQNTFKIQRRFFQ
AVIMVNITKPSSHQGR*CDK*THHSEANYLMEYK
SNGHPYFLILAFISLSFGWLVNGEELSG*FNQL
QTICPCPKMMSQISIKPVLVSPS*YCSLRHSLS
LILRSCFKRHNHFELP*NLSSTTTTLGEKNMLKE
VSWLNNSL*PISTVAISTQRKGPKSLCSLKISV
NLKEQKQDRTSGTLIRMIPNKSVICELSGLFGY
FNLHIFSSYIGLYLWPCSSFQC*SAKQKLLLMMC
E*TYATAWPFFLQSLLSFSLY*TLQYAQKLSFI
K*KEKNIWLIFHCS*SFIQ*SCKKIS*ILNITL
SRFLKSKSFQ*KVSYFILLY*VVKNVGLFTITCS
LISEIYNSILRP**DSSIPCSTYGVL*ELIVGD
ELLLILFSSFHCWFIADLYPVSNSRYY**TFST
SSKKFKFFSVTVTENTICI*HLCSNKCAIFFLTQ
*

Frame 1

PEASRARW*GRLSEKNVRLKRPTSSIRGDRQPA
PGGRGGQARKPPPPPRPESSCGAK*TEPLGGAR
GSPSPFSRPK*TLIGVVGCGDGAAEVAEDESPAT
THGRYRDRQGAERPTGLQCDVFPTC**WEN*TN
YTGKQSIQWIRY**KRRYNILGNKWSTKSFL*F
I*RFYRQPSLQ*SLE*QSIRSP*RLVPNTFHELK
SDGKNIRERLIFPVQQRYWITRLSI*SPETRSG
AWEPSTAIFFRKTWDSILFILCYKFQEETTP*L
CSA*SLASKKSQKGASWFAFFCICTIPKFR*FQP
*IS*LSIS*ATNQYVR*HFLYARWDPQFF*PLE
FIKWDEPAWFWWNSGDLHFPHVSIQ*LWQPSFT
*PLELSSTLSFTNRHKHESSTNVQLSSRQYQQFT
LRCCLTHSSHQWIRQHSRNQRECCWKLTDR*CT
WKGFGIYLFS*PYQQ*FSVKSINTSWITFTSHR
YQSVAKTWRASTFIPKL*KLTPLPAVSNGGSFRQ
TG*CNPCAAEPCCGTFHQFACWSQ*YT*FIGTI
P*CTNWKPQFKLWRIKPCCKQSISFNGWNSSGR
LCQSQWQSFSPV*YSHYFKHRPEP*NTRKL*RWL
AKSVWNCCYNRNQD*KQRKG*KPS*TSFIR*HE
VR**ILPKRYQGFI*RQNKQY**R*GFEP*TED
RKGEGEADG*QCQRTLTRAGY**SIQRAWPNVSA
SLEE*KTPNKTPYSSSSRGSHP*SRTASQREEP
*PQSSLP*EKGRRKSFCRIGRAANHTARNPSWA
**NYQPYGSYVNISQFQSYQ*AR*KVTSPHKDLD
NSDYLKTQT*QEGEEKTKHLNQETQM*SYDQSN
WSTLPSEVKIGSSSDRTSAHEMFATNLLLQAVC
RFCTIRDCLDLSTHRGSCLVPKLN*QMHCNYKFY
LLL*NCKVYI*REKVNVES*STLS*CQHTLKRF
TCREQSSDNHWPLAFPAYLLVS*KGREKSFVAL
SYPLAI*IAFSMK*ENITWYSISLALIF*FIFIF
SLWVLYLISKSEQFMVTVQPPPCSPVCFAHLPY
SGKQRPSVTIA*LAF*KGKFCSLGFPPNKHCFI
SNNNQDFSSF*IS*ESL**QNCKLQGKNLLFRNR
FVFQAVSTTYSTCKVLAVSKHKIHLKYKDDFFR
L*LW*T*QNPVVTKAGSVINEHTTLRLIT*WNT
RAMVTRISLS*PLFLCHLDGWSMGKN*VGDLINC
KPSAPVPK**ARLALNQYLSVHLNTVH*GTLCL
*SLGVVLKDIITLNFHETCLPPQQPWERKTC*R
RYLGLIIPYSQYQQWQSAHRGKDPNHYVA*RFLL
I*KNKNKTELLVL*SG*FLTSQSFVNLVDFLVT
LICIYSPVTSDSICGLVLHFSVNQLNRSCCL*C
VSEHMPLPGLFFFRACCLFRYIRLCSMPRSFPS*
NRKKKTFGLFFTVASLLYNNLVRKFLEF*ILLF
LDF*NQKVFSKKFLTLFYYIR**KM*GYLP*PV
H*YQKFTIAF*DHSRILAYRVVPMEYCKS*LLEM
NCFSSCSPVSIVGLLQICILCQIQGIIDKPFQP
AARSSNFFLSL*QKTQYVYNIYVAINVPSFF*H
SK

Frame 2

RRRAERGGEAASAKKMSA*RDPQVLFGGTDSPP
REEGAARPESRLPLPDPRARAGQSEPSRWAVQG
EAQARSPGQSEL*SGWLDAETGRQKWPKMNPQQ
QRMAAIGTDKELSDLLDFSAMFSPPVNSGKTRPT
TLGSSQFSGSGIDERGGTTSWGTSGQPSPSYDS
SRGFTDSPHYSDHLNDSRLGAHEGLSPTPFMNS
NLMGKTSERGSFSLYSRDTGLPGCQSSLLRQDLG
LGSPAQLSSSGKPGTAYYSFSATSSRRRPLHDS
AALDPLQAKKVRKVPPGLPSSVYAPSPNSDDFN
RESPSYPSPKPPTSMFASTFFMQDGTHNSSDLWS
SSNGMSQPGFGGILGTSTSHMSQSSSYGNLHSH
DRLSYPPHSVSPTDINTSLPPMSSFHRGSTSSS
PYVAASHTPPINGSDSILGTRGNAAGSSQTGDAL
GKALASIYSPDHTSSSFPSNPSTPVGSPSPLTG
TSQWPRPGGQAPSSPSYENSLHSLQSRMEDRLD
RLDDAIHVLRNHAVGPSTSLPAGHSDIHSLLGPS
HNAPIGSLNSNYGGSSLVASSRSASMVGTHRED
SVSLNGNHSVLSSTVTTSSTDLNHKTQENYRGG
LQSQSGTVVTTEIKTENKEKDENLHEPPSSDDMK
SDDESSQKDIKVSSRGRTSSTNEDEDLNPEQKI
EREKERRMANNARERLRVRDINEAFKELGRMCQ
LHLKSEKPQTKLLILHQAVAVILSLEQQVRERNL
NPKAACLKRREEEKVSAVSAEPPTTLPGTHPGL
SETTNPMGHM*TSASSRVISRLDRR*PLLIRTW
TTQII*RHKPDRREKKKQNT*TKKLKCNPTIKAT
GQHFHQK*R*EAHQIEHQPMRCLQQIFCCKQCV
ASAQSETVSISPLTVEVALCLN*IDKCIVTTNF
IYCYETVRSTYKGKKLMWKADLHSADASIH*SGS
RAENKAVTTIGP*HSRHTY*CLKKEGKSLLLPS
PILLPYE*RFP*NRKILLGIAFLLLSFFDLFLF
SLCGCYI*SLNLNSLWSQSSLLRAALCALHIYLT
VVSRDHL*P*PS*HFKRGNFVL*VFPQINIALF
LIITKTFQASRSHRKACNSKIVNYKGRIYFLEI
ALFSKQ*VLHTVLVKC*L*VSTKYI*NTKTIFSG
CDYGEHNKTQ*SPRQVV**MNTPL*G*LPNGIQ
EQWSPVFPYPSLYFSVIWMAGQWGRIEWVI*ST
ANHLPLSQNDEPD*H*TSTCQSILILFIKALSVS
NP*ELF*KT*SL*TSMKPVFHHNNPGREKHAKG
GILA**FLIANINSGNQHTEERTQITM*LKDFC
*FERTKTRQNFWYSNQDDS*QVSHL*T*WTFWLL
*FAYILQLHRTLSVALFFISVLIS*TEVVAYDV
*VNICHCLAFFSSELVVFFAILDFAVCPEAFLH
KIERKKHLAYFSL*LVFYTIIL*ENFLNSKYYSF
*IFEIKKFSVKSFLLYFIILGSKKCRVIYHNLF
INIRNLQ*HFKTIVGF*HTV*YLWSIVRANCWR
*IASHLVLQFPLLVYCRFVSCVKFKVLLINLFNQ
QQEVQIFFCHCNRKHNMYITFM*Q*MCHLFFNT
VK

Sequence NM_207040

TGGATATATGTCTTGGAGAGAGGCTGTGTGCATTAGCTACAAATAGTAACATTTTCAAAATCAACATGTATTGTGCTTATCCTGTCCCTGGAATGGGCAG
CAATTCTTTGATGTATTACTACAATGGGAAAACGGTATATGCACCATCCCCAAATTCAGATGATTTCAACCGTGAATCTCCTAGTTATCCATCTCCTAAG
CCACCAACCAGTATGTTCGCTAGCACTTTCTTTATGCAAGATGGGACCCACAATTCTTCTGACCTTTGGAGTTCATCAAATGGGATGAGCCAGCCTGGTT
TTGGTGGAATTCTGGGGACCTCCACTTCCCACATGTCTCAATCCAGTAGTTATGGCAACCTTCATTCACATGACCGCTTGAGTTATCCTCCACACTCAGT
TTCACCAACAGACATAAACACGAGTCTTCCACCAATGTCCAGCTTTCATCGCGGCAGTACCAGCAGTTCACCTTACGTTGCTGCCTCACACACTCCTCCC
ATCAATGGATCAGACAGCATTCTAGGAACCAGAGGGAATGCTGCTGGAAGCTCACAGACAGGTGATGCACTTGGAAAGGCTTTGGCATCTATTTATTCTC
CTGACCATACCAGCAGTAGTTTTCCGTCAAATCCATCAACACCAGTTGGATCACCTTCACCTCTCACAGGTACCAGTCAGTGGCCAAGACCTGGAGGGCA
AGCACCTTCATCCCCAAGCTATGAAAACTCACTCCACTCCCTGCAGTCTCGAATGGAGGATCGTTTAGACAGACTGGATGATGCAATCCATGTGCTGCGG
AACCATGCTGTGGGACCTTCCACCAGTTTGCCTGCTGGTCACAGTGATATACATAGTTTATTGGGACCATCCCATAATGCACCAATTGGAAGCCTCAATT
CAAACTATGGAGGATCAAGCCTTGTTGCAAGCAGTCGATCAGCTTCAATGGTTGGAACTCATCGGGAAGACTCTGTCAGTCTCAATGGCAATCATTCAGT
CCTGTCTAGTACAGTCACTACTTCAAGCACAGACCTGAACCATAAAACACAAGAAAATTATAGAGGTGGCTTGCAAAGTCAGTCTGGAACTGTTGTTACA
ACAGAAATCAAGACTGAAAACAAAGAAAAGGATGAAAACCTTCATGAACCTCCTTCATCAGATGACATGAAGTCAGATGATGAATCCTCCCAAAAAGATA
TCAAGGTTTCATCTAGAGGCAGAACAAGCAGTACTAATGAAGATGAGGATTTGAACCCTGAACAGAAGATAGAAAGGGAGAAGGAGAGGCGGATGGCTAA
CAATGCCAGAGAACGCTTACGCGTGCGGGATATTAATGAAGCATTCAAAGAGCTTGGCCGAATGTGTCAGCTTCACTTGAAGAGTGAAAAACCCCAAACA
AAACTCCTTATTCTTCATCAAGCCGTGGCAGTCATCCTTAGTCTAGAACAGCAAGTCAGAGAGAGGAACCTTAACCCCAAAGCAGCCTGCCTTAAGAGAA
GGGAAGAAGAAAAAGTTTCTGCCGTATCGGCAGAGCCGCCAACCACACTGCCAGGAACCCATCCTGGGCTTAGTGAAACTACCAACCCTATGGGTCATAT
GTAAACATCAGCCAGTTCCAGAGTTATCAGTAGGCTAGATAGAAGGTGACCTCTCCTCATAAGGACTTGGACAACTCAGATTATCTGAAGACACAAACCT
GACAGGAGGGAGAAGAAAAAACAAAACACTTGAACCAAGAAACTCAAATGTAATCCTACGATCAAAGCAACTGGTCAACACTTCCATCAGAAGTGAAGAT
AGGAAGCTCATCAGATAGAACATCAGCCCATGAGATGTTTGCAACAAATCTTTTGTTGCAAGCAGTGTGTCGCTTCTGCACAATCAGAGACTGTCTCGAT
CTCTCCACTCACCGTGGAAGTTGCCTTGTGCCTAAACTGAATTGACAAATGCATTGTAACTACAAATTTTATTTATTGTTATGAAACTGTAAGGTCTACA
TATAAAGGGAAAAAGTTAATGTGGAAAGCTGATCTACACTCAGCTGATGCCAGCATACATTAAAGCGGTTCACGTGCAGAGAACAAAGCAGTGACAACCA
TTGGCCCTTAGCATTCCCGGCATACCTATTAGTGTCTTAAAAAGGAAGGGAAAAGTCTTTTGTTGCCCTCTCCTATCCTCTTGCCATATGAATAGCGTTT
TCCATGAAATAGGAAAATATTACTTGGTATAGCATTTCTCTTGCTCTCATTTTTTGATTTATTTTTATTTTCTCTTTGTGGGTGTTATATTTGATCTCTA
AATCTGAACAGTTTATGGTCACAGTCCAGCCTCCTCCGTGCAGCCCTGTGTGCTTTGCACATTTACCTTACAGTGGTAAGCAGAGACCATCTGTGACCAT
AGCCTAGCTAGCATTTTAAAAGGGGAAATTTTGTTCTCTAGGTTTTCCCCCAAATAAACATTGCTTTATTTCTAATAATAACCAAGACTTTTCAAGCTTC
TAGATCTCATAGGAAAGCTTGTAATAGCAAAATTGTAAATTACAAGGGAAGAATCTACTTTTTAGAAATCGCTTTGTTTTCCAAGCAGTAAGTACTACAT
ACAGTACTTGTAAAGTGTTAGCTGTAAGTAAGCACAAAATACATTTAAAATACAAAGACGATTTTTTCAGGCTGTGATTATGGTGAACATAACAAAACCC
AGTAGTCACCAAGGCAGGTAGTGTGATAAATGAACACACCACTCTGAGGCTAATTACCTAATGGAATACAAGAGCAATGGTCACCCGTATTTCCTTATCC
TAGCCTTTATTTCTCTGTCATTTGGATGGCTGGTCAATGGGGAAGAATTGAGTGGGTGATTTAATCAACTGCAAACCATCTGCCCCTGTCCCAAAATGAT
GAGCCAGATTAGCATTAAACCAGTACTTGTCAGTCCATCTTAATACTGTTCATTAAGGCACTCTCTGTCTCTAATCCTTAGGAGTTGTTTTAAAAGACAT
AATCACTTTGAACTTCCATGAAACCTGTCTTCCACCACAACAACCCTGGGAGAGAAAAACATGCTAAAGGAGGTATCTTGGCTTAATAATTCCTTATAGC
CAATATCAACAGTGGCAATCAGCACACAGAGGAAAGGACCCAAATCACTATGTAGCTTAAAGATTTCTGTTAATTTGAAAGAACAAAAACAAGACAGAAC
TTCTGGTACTCTAATCAGGATGATTCCTAACAAGTCAGTCATTTGTGAACTTAGTGGACTTTTTGGTTACTTTAATTTGCATATATTCTCCAGTTACATC
GGACTCTATCTGTGGCCTTGTTCTTCATTTCAGTGTTAATCAGCTAAACAGAAGTTGTTGCTTATGATGTGTGAGTGAACATATGCCACTGCCTGGCCTT
TTTTTCTTCAGAGCTTGTTGTCTTTTTCGCTATATTAGACTTTGCAGTATGCCCAGAAGCTTTCCTTCATAAAATAGAAAGAAAAAAACATTTGGCTTAT
TTTTCACTGTAGCTAGTCTTTTATACAATAATCTTGTAAGAAAATTTCTTGAATTCTAAATATTACTCTTTCTAGATTTTTGAAATCAAAAAGTTTTCAG
TAAAAAGTTTCTTACTTTATTTTATTATATTAGGTAGTAAAAAATGTAGGGTTATTTACCATAACCTGTTCATTAATATCAGAAATTTACAATAGCATTT
TAAGACCATAGTAGGATTCTAGCATACCGTGTAGTACCTATGGAGTATTGTAAGAGCTAATTGTTGGAGATGAATTGCTTCTCATCTTGTTCTCCAGTTT
CCATTGTTGGTTTATTGCAGATTTGTATCCTGTGTCAAATTCAAGGTATTATTGATAAACCTTTTCAACCAGCAGCAAGAAGTTCAAATTTTTTTCTGTC
ACTGTAACAGAAAACACAATATGTATATAACATTTATGTAGCAATAAATGTGCCATCTTTTTTTTAACACAGTAAAA
  • refseq_TCF12.F2 (forward primer)
  • refseq_TCF12.R2 (reverse primer)
  • Single exon skipping

Frame 0

WIYVLERGCVH*LQIVTFSKSTCIVLILSLEWA
AIL*CITTMGKRYMHHPQIQMISTVNLLVIHLLS
HQPVCSLALSLCKMGPTILLTFGVHQMG*ASLV
LVEFWGPPLPTCLNPVVMATFIHMTA*VILHTQ
FHQQT*TRVFHQCPAFIAAVPAVHLTLLPHTLLP
SMDQTAF*EPEGMLLEAHRQVMHLERLWHLFIL
LTIPAVVFRQIHQHQLDHLHLSQVPVSGQDLEG
KHLHPQAMKTHSTPCSLEWRIV*TDWMMQSMCCG
TMLWDLPPVCLLVTVIYIVYWDHPIMHQLEASI
QTMEDQALLQAVDQLQWLELIGKTLSVSMAIIQ
SCLVQSLLQAQT*TIKHKKIIEVACKVSLELLLQ
QKSRLKTKKRMKTFMNLLHQMT*SQMMNPPKKI
SRFHLEAEQAVLMKMRI*TLNRR*KGRRRGGWL
TMPENAYACGILMKHSKSLAECVSFT*RVKNPKQ
NSLFFIKPWQSSLV*NSKSERGTLTPKQPALRE
GKKKKFLPYRQSRQPHCQEPILGLVKLPTLWVI
CKHQPVPELSVG*IEGDLSS*GLGQLRLSEDTNL
TGGRRKNKTLEPRNSNVILRSKQLVNTSIRSED
RKLIR*NISP*DVCNKSFVASSVSLLHNQRLSR
SLHSPWKLPCA*TELTNAL*LQILFIVMKL*GLH
IKGKS*CGKLIYTQLMPAYIKAVHVQRTKQ*QP
LALSIPGIPISVLKRKGKVFCCPLLSSCHMNSV
FHEIGKYYLV*HFSCSHFLIYFYFLFVGVIFDL*
I*TVYGHSPASSVQPCVLCTFTLQW*AETICDH
SLASILKGEILFSRFSPK*TLLYF***PRLFKL
LDLIGKLVIAKL*ITREESTF*KSLCFPSSKYYI
QYL*SVSCK*AQNTFKIQRRFFQAVIMVNITKP
SSHQGR*CDK*THHSEANYLMEYKSNGHPYFLI
LAFISLSFGWLVNGEELSG*FNQLQTICPCPKMM
SQISIKPVLVSPS*YCSLRHSLSLILRSCFKRH
NHFELP*NLSSTTTTLGEKNMLKEVSWLNNSL*
PISTVAISTQRKGPKSLCSLKISVNLKEQKQDRT
SGTLIRMIPNKSVICELSGLFGYFNLHIFSSYI
GLYLWPCSSFQC*SAKQKLLLMMCE*TYATAWP
FFLQSLLSFSLY*TLQYAQKLSFIK*KEKNIWLI
FHCS*SFIQ*SCKKIS*ILNITLSRFLKSKSFQ
*KVSYFILLY*VVKNVGLFTITCSLISEIYNSI
LRP**DSSIPCSTYGVL*ELIVGDELLLILFSSF
HCWFIADLYPVSNSRYY**TFSTSSKKFKFFSV
TVTENTICI*HLCSNKCAIFFLTQ*

Frame 1

GYMSWREAVCISYK**HFQNQHVLCLSCPWNGQ
QFFDVLLQWENGICTIPKFR*FQP*IS*LSIS*
ATNQYVR*HFLYARWDPQFF*PLEFIKWDEPAWF
WWNSGDLHFPHVSIQ*LWQPSFT*PLELSSTLS
FTNRHKHESSTNVQLSSRQYQQFTLRCCLTHSS
HQWIRQHSRNQRECCWKLTDR*CTWKGFGIYLFS
*PYQQ*FSVKSINTSWITFTSHRYQSVAKTWRA
STFIPKL*KLTPLPAVSNGGSFRQTG*CNPCAA
EPCCGTFHQFACWSQ*YT*FIGTIP*CTNWKPQF
KLWRIKPCCKQSISFNGWNSSGRLCQSQWQSFS
PV*YSHYFKHRPEP*NTRKL*RWLAKSVWNCCY
NRNQD*KQRKG*KPS*TSFIR*HEVR**ILPKRY
QGFI*RQNKQY**R*GFEP*TEDRKGEGEADG*
QCQRTLTRAGY**SIQRAWPNVSASLEE*KTPN
KTPYSSSSRGSHP*SRTASQREEP*PQSSLP*EK
GRRKSFCRIGRAANHTARNPSWA**NYQPYGSY
VNISQFQSYQ*AR*KVTSPHKDLDNSDYLKTQT
*QEGEEKTKHLNQETQM*SYDQSNWSTLPSEVKI
GSSSDRTSAHEMFATNLLLQAVCRFCTIRDCLD
LSTHRGSCLVPKLN*QMHCNYKFYLLL*NCKVY
I*REKVNVES*STLS*CQHTLKRFTCREQSSDNH
WPLAFPAYLLVS*KGREKSFVALSYPLAI*IAF
SMK*ENITWYSISLALIF*FIFIFSLWVLYLIS
KSEQFMVTVQPPPCSPVCFAHLPYSGKQRPSVTI
A*LAF*KGKFCSLGFPPNKHCFISNNNQDFSSF
*IS*ESL**QNCKLQGKNLLFRNRFVFQAVSTT
YSTCKVLAVSKHKIHLKYKDDFFRL*LW*T*QNP
VVTKAGSVINEHTTLRLIT*WNTRAMVTRISLS
*PLFLCHLDGWSMGKN*VGDLINCKPSAPVPK*
*ARLALNQYLSVHLNTVH*GTLCL*SLGVVLKDI
ITLNFHETCLPPQQPWERKTC*RRYLGLIIPYS
QYQQWQSAHRGKDPNHYVA*RFLLI*KNKNKTE
LLVL*SG*FLTSQSFVNLVDFLVTLICIYSPVTS
DSICGLVLHFSVNQLNRSCCL*CVSEHMPLPGL
FFFRACCLFRYIRLCSMPRSFPS*NRKKKTFGL
FFTVASLLYNNLVRKFLEF*ILLFLDF*NQKVFS
KKFLTLFYYIR**KM*GYLP*PVH*YQKFTIAF
*DHSRILAYRVVPMEYCKS*LLEMNCFSSCSPV
SIVGLLQICILCQIQGIIDKPFQPAARSSNFFLS
L*QKTQYVYNIYVAINVPSFF*HSK

Frame 2

DICLGERLCALATNSNIFKINMYCAYPVPGMGS
NSLMYYYNGKTVYAPSPNSDDFNRESPSYPSPK
PPTSMFASTFFMQDGTHNSSDLWSSSNGMSQPG
FGGILGTSTSHMSQSSSYGNLHSHDRLSYPPHSV
SPTDINTSLPPMSSFHRGSTSSSPYVAASHTPP
INGSDSILGTRGNAAGSSQTGDALGKALASIYS
PDHTSSSFPSNPSTPVGSPSPLTGTSQWPRPGGQ
APSSPSYENSLHSLQSRMEDRLDRLDDAIHVLR
NHAVGPSTSLPAGHSDIHSLLGPSHNAPIGSLN
SNYGGSSLVASSRSASMVGTHREDSVSLNGNHSV
LSSTVTTSSTDLNHKTQENYRGGLQSQSGTVVT
TEIKTENKEKDENLHEPPSSDDMKSDDESSQKD
IKVSSRGRTSSTNEDEDLNPEQKIEREKERRMAN
NARERLRVRDINEAFKELGRMCQLHLKSEKPQT
KLLILHQAVAVILSLEQQVRERNLNPKAACLKR
REEEKVSAVSAEPPTTLPGTHPGLSETTNPMGHM
*TSASSRVISRLDRR*PLLIRTWTTQII*RHKP
DRREKKKQNT*TKKLKCNPTIKATGQHFHQK*R
*EAHQIEHQPMRCLQQIFCCKQCVASAQSETVSI
SPLTVEVALCLN*IDKCIVTTNFIYCYETVRST
YKGKKLMWKADLHSADASIH*SGSRAENKAVTT
IGP*HSRHTY*CLKKEGKSLLLPSPILLPYE*RF
P*NRKILLGIAFLLLSFFDLFLFSLCGCYI*SL
NLNSLWSQSSLLRAALCALHIYLTVVSRDHL*P
*PS*HFKRGNFVL*VFPQINIALFLIITKTFQAS
RSHRKACNSKIVNYKGRIYFLEIALFSKQ*VLH
TVLVKC*L*VSTKYI*NTKTIFSGCDYGEHNKT
Q*SPRQVV**MNTPL*G*LPNGIQEQWSPVFPYP
SLYFSVIWMAGQWGRIEWVI*STANHLPLSQND
EPD*H*TSTCQSILILFIKALSVSNP*ELF*KT
*SL*TSMKPVFHHNNPGREKHAKGGILA**FLIA
NINSGNQHTEERTQITM*LKDFC*FERTKTRQN
FWYSNQDDS*QVSHL*T*WTFWLL*FAYILQLH
RTLSVALFFISVLIS*TEVVAYDV*VNICHCLAF
FSSELVVFFAILDFAVCPEAFLHKIERKKHLAY
FSL*LVFYTIIL*ENFLNSKYYSF*IFEIKKFS
VKSFLLYFIILGSKKCRVIYHNLFINIRNLQ*HF
KTIVGF*HTV*YLWSIVRANCWR*IASHLVLQF
PLLVYCRFVSCVKFKVLLINLFNQQQEVQIFFC
HCNRKHNMYITFM*Q*MCHLFFNTVK

Sequence NM_207036

TCCGGAGGCGAGCCGAGCGCGGTGGTGAGGCCGCCTCAGCGAAAAAAATGTCCGCCTGAAGAGACCCACAAGTTCTATTCGGGGGGACCGACAGCCCGCC
CCGGGAGGAAGGGGCGGCCAGGCCCGAAAGCCGCCTCCCCCTCCCAGACCCGAGAGCTCGTGCGGGGCAAAGTGAACCGAGCCGCTGGGCGGTGCAAGGG
GAAGCCCAAGCCCGTTCTCCCGGCCAAAGTGAACTTTAATCGGGGTGGTTGGATGCGGAGACGGGGCGGCAGAAGTGGCCGAAGATGAATCCCCAGCAAC
AACGCATGGCCGCTATAGGGACCGACAAGGAGCTGAGCGACCTACTGGACTTCAGTGCGATGTTTTCCCCACCTGTTAATAGTGGGAAAACTAGACCAAC
TACACTGGGAAGCAGTCAATTCAGTGGATCAGGTATTGATGAAAGAGGAGGTACAACATCTTGGGGAACAAGTGGTCAACCAAGTCCTTCCTATGATTCA
TCTAGAGGTTTTACAGACAGCCCTCATTACAGTGATCACTTGAATGACAGTCGATTAGGAGCCCATGAAGGCTTGTCCCCAACACCTTTCATGAACTCAA
ATCTGATGGGAAAAACATCAGAGAGAGGCTCATTTTCCCTGTACAGCAGAGATACTGGATTACCAGGCTGTCAATCTAGTCTCCTGAGACAAGATCTGGG
GCTTGGGAGCCCAGCACAGCTATCTTCTTCAGGAAAACCTGGGACAGCATACTATTCATTCTCTGCTACAAGTTCCAGGAGGAGACCACTCCATGACTCT
GCAGCGCTTGATCCCTTGCAAGCAAAAAAAGTCAGAAAGGTGCCTCCTGGTTTGCCTTCTTCTGTATATGCACCATCCCCAAATTCAGATGATTTCAACC
GTGAATCTCCTAGTTATCCATCTCCTAAGCCACCAACCAGTATGTTCGCTAGCACTTTCTTTATGCAAGATGGGACCCACAATTCTTCTGACCTTTGGAG
TTCATCAAATGGGATGAGCCAGCCTGGTTTTGGTGGAATTCTGGGGACCTCCACTTCCCACATGTCTCAATCCAGTAGTTATGGCAACCTTCATTCACAT
GACCGCTTGAGTTATCCTCCACACTCAGTTTCACCAACAGACATAAACACGAGTCTTCCACCAATGTCCAGCTTTCATCGCGGCAGTACCAGCAGTTCAC
CTTACGTTGCTGCCTCACACACTCCTCCCATCAATGGATCAGACAGCATTCTAGGAACCAGAGGGAATGCTGCTGGAAGCTCACAGACAGGTGATGCACT
TGGAAAGGCTTTGGCATCTATTTATTCTCCTGACCATACCAGCAGTAGTTTTCCGTCAAATCCATCAACACCAGTTGGATCACCTTCACCTCTCACAGGT
ACCAGTCAGTGGCCAAGACCTGGAGGGCAAGCACCTTCATCCCCAAGCTATGAAAACTCACTCCACTCCCTGAAAAATCGAGTTGAGCAGCAACTTCACG
AGCATTTGCAAGATGCAATGTCCTTCTTAAAGGATGTCTGTGAGCAGTCTCGAATGGAGGATCGTTTAGACAGACTGGATGATGCAATCCATGTGCTGCG
GAACCATGCTGTGGGACCTTCCACCAGTTTGCCTGCTGGTCACAGTGATATACATAGTTTATTGGGACCATCCCATAATGCACCAATTGGAAGCCTCAAT
TCAAACTATGGAGGATCAAGCCTTGTTGCAAGCAGTCGATCAGCTTCAATGGTTGGAACTCATCGGGAAGACTCTGTCAGTCTCAATGGCAATCATTCAG
TCCTGTCTAGTACAGTCACTACTTCAAGCACAGACCTGAACCATAAAACACAAGAAAATTATAGAGGTGGCTTGCAAAGTCAGTCTGGAACTGTTGTTAC
AACAGAAATCAAGACTGAAAACAAAGAAAAGGATGAAAACCTTCATGAACCTCCTTCATCAGATGACATGAAGTCAGATGATGAATCCTCCCAAAAAGAT
ATCAAGGTTTCATCTAGAGGCAGAACAAGCAGTACTAATGAAGATGAGGATTTGAACCCTGAACAGAAGATAGAAAGGGAGAAGGAGAGGCGGATGGCTA
ACAATGCCAGAGAACGCTTACGCGTGCGGGATATTAATGAAGCATTCAAAGAGCTTGGCCGAATGTGTCAGCTTCACTTGAAGAGTGAAAAACCCCAAAC
AAAACTCCTTATTCTTCATCAAGCCGTGGCAGTCATCCTTAGTCTAGAACAGCAAGTCAGAGAGAGGAACCTTAACCCCAAAGCAGCCTGCCTTAAGAGA
AGGGAAGAAGAAAAAGTTTCTGCCGTATCGGCAGAGCCGCCAACCACACTGCCAGGAACCCATCCTGGGCTTAGTGAAACTACCAACCCTATGGGTCATA
TGTAAACATCAGCCAGTTCCAGAGTTATCAGTAGGCTAGATAGAAGGTGACCTCTCCTCATAAGGACTTGGACAACTCAGATTATCTGAAGACACAAACC
TGACAGGAGGGAGAAGAAAAAACAAAACACTTGAACCAAGAAACTCAAATGTAATCCTACGATCAAAGCAACTGGTCAACACTTCCATCAGAAGTGAAGA
TAGGAAGCTCATCAGATAGAACATCAGCCCATGAGATGTTTGCAACAAATCTTTTGTTGCAAGCAGTGTGTCGCTTCTGCACAATCAGAGACTGTCTCGA
TCTCTCCACTCACCGTGGAAGTTGCCTTGTGCCTAAACTGAATTGACAAATGCATTGTAACTACAAATTTTATTTATTGTTATGAAACTGTAAGGTCTAC
ATATAAAGGGAAAAAGTTAATGTGGAAAGCTGATCTACACTCAGCTGATGCCAGCATACATTAAAGCGGTTCACGTGCAGAGAACAAAGCAGTGACAACC
ATTGGCCCTTAGCATTCCCGGCATACCTATTAGTGTCTTAAAAAGGAAGGGAAAAGTCTTTTGTTGCCCTCTCCTATCCTCTTGCCATATGAATAGCGTT
TTCCATGAAATAGGAAAATATTACTTGGTATAGCATTTCTCTTGCTCTCATTTTTTGATTTATTTTTATTTTCTCTTTGTGGGTGTTATATTTGATCTCT
AAATCTGAACAGTTTATGGTCACAGTCCAGCCTCCTCCGTGCAGCCCTGTGTGCTTTGCACATTTACCTTACAGTGGTAAGCAGAGACCATCTGTGACCA
TAGCCTAGCTAGCATTTTAAAAGGGGAAATTTTGTTCTCTAGGTTTTCCCCCAAATAAACATTGCTTTATTTCTAATAATAACCAAGACTTTTCAAGCTT
CTAGATCTCATAGGAAAGCTTGTAATAGCAAAATTGTAAATTACAAGGGAAGAATCTACTTTTTAGAAATCGCTTTGTTTTCCAAGCAGTAAGTACTACA
TACAGTACTTGTAAAGTGTTAGCTGTAAGTAAGCACAAAATACATTTAAAATACAAAGACGATTTTTTCAGGCTGTGATTATGGTGAACATAACAAAACC
CAGTAGTCACCAAGGCAGGTAGTGTGATAAATGAACACACCACTCTGAGGCTAATTACCTAATGGAATACAAGAGCAATGGTCACCCGTATTTCCTTATC
CTAGCCTTTATTTCTCTGTCATTTGGATGGCTGGTCAATGGGGAAGAATTGAGTGGGTGATTTAATCAACTGCAAACCATCTGCCCCTGTCCCAAAATGA
TGAGCCAGATTAGCATTAAACCAGTACTTGTCAGTCCATCTTAATACTGTTCATTAAGGCACTCTCTGTCTCTAATCCTTAGGAGTTGTTTTAAAAGACA
TAATCACTTTGAACTTCCATGAAACCTGTCTTCCACCACAACAACCCTGGGAGAGAAAAACATGCTAAAGGAGGTATCTTGGCTTAATAATTCCTTATAG
CCAATATCAACAGTGGCAATCAGCACACAGAGGAAAGGACCCAAATCACTATGTAGCTTAAAGATTTCTGTTAATTTGAAAGAACAAAAACAAGACAGAA
CTTCTGGTACTCTAATCAGGATGATTCCTAACAAGTCAGTCATTTGTGAACTTAGTGGACTTTTTGGTTACTTTAATTTGCATATATTCTCCAGTTACAT
CGGACTCTATCTGTGGCCTTGTTCTTCATTTCAGTGTTAATCAGCTAAACAGAAGTTGTTGCTTATGATGTGTGAGTGAACATATGCCACTGCCTGGCCT
TTTTTTCTTCAGAGCTTGTTGTCTTTTTCGCTATATTAGACTTTGCAGTATGCCCAGAAGCTTTCCTTCATAAAATAGAAAGAAAAAAACATTTGGCTTA
TTTTTCACTGTAGCTAGTCTTTTATACAATAATCTTGTAAGAAAATTTCTTGAATTCTAAATATTACTCTTTCTAGATTTTTGAAATCAAAAAGTTTTCA
GTAAAAAGTTTCTTACTTTATTTTATTATATTAGGTAGTAAAAAATGTAGGGTTATTTACCATAACCTGTTCATTAATATCAGAAATTTACAATAGCATT
TTAAGACCATAGTAGGATTCTAGCATACCGTGTAGTACCTATGGAGTATTGTAAGAGCTAATTGTTGGAGATGAATTGCTTCTCATCTTGTTCTCCAGTT
TCCATTGTTGGTTTATTGCAGATTTGTATCCTGTGTCAAATTCAAGGTATTATTGATAAACCTTTTCAACCAGCAGCAAGAAGTTCAAATTTTTTTCTGT
CACTGTAACAGAAAACACAATATGTATATAACATTTATGTAGCAATAAATGTGCCATCTTTTTTTTAACACAGTAAAA
  • refseq_TCF12.F2 (forward primer)
  • refseq_TCF12.R2 (reverse primer)
  • Single exon skipping

Frame 0

SGGEPSAVVRPPQRKKCPPEETHKFYSGGPTAR
PGRKGRPGPKAASPSQTRELVRGKVNRAAGRCKG
KPKPVLPAKVNFNRGGWMRRRGGRSGRR*IPSN
NAWPL*GPTRS*ATYWTSVRCFPHLLIVGKLDQ
LHWEAVNSVDQVLMKEEVQHLGEQVVNQVLPMIH
LEVLQTALITVIT*MTVD*EPMKACPQHLS*TQ
I*WEKHQREAHFPCTAEILDYQAVNLVS*DKIW
GLGAQHSYLLQENLGQHTIHSLLQVPGGDHSMTL
QRLIPCKQKKSERCLLVCLLLYMHHPQIQMIST
VNLLVIHLLSHQPVCSLALSLCKMGPTILLTFG
VHQMG*ASLVLVEFWGPPLPTCLNPVVMATFIHM
TA*VILHTQFHQQT*TRVFHQCPAFIAAVPAVH
LTLLPHTLLPSMDQTAF*EPEGMLLEAHRQVMH
LERLWHLFILLTIPAVVFRQIHQHQLDHLHLSQV
PVSGQDLEGKHLHPQAMKTHSTP*KIELSSNFT
SICKMQCPS*RMSVSSLEWRIV*TDWMMQSMCC
GTMLWDLPPVCLLVTVIYIVYWDHPIMHQLEASI
QTMEDQALLQAVDQLQWLELIGKTLSVSMAIIQ
SCLVQSLLQAQT*TIKHKKIIEVACKVSLELLL
QQKSRLKTKKRMKTFMNLLHQMT*SQMMNPPKKI
SRFHLEAEQAVLMKMRI*TLNRR*KGRRRGGWL
TMPENAYACGILMKHSKSLAECVSFT*RVKNPK
QNSLFFIKPWQSSLV*NSKSERGTLTPKQPALRE
GKKKKFLPYRQSRQPHCQEPILGLVKLPTLWVI
CKHQPVPELSVG*IEGDLSS*GLGQLRLSEDTN
LTGGRRKNKTLEPRNSNVILRSKQLVNTSIRSED
RKLIR*NISP*DVCNKSFVASSVSLLHNQRLSR
SLHSPWKLPCA*TELTNAL*LQILFIVMKL*GL
HIKGKS*CGKLIYTQLMPAYIKAVHVQRTKQ*QP
LALSIPGIPISVLKRKGKVFCCPLLSSCHMNSV
FHEIGKYYLV*HFSCSHFLIYFYFLFVGVIFDL
*I*TVYGHSPASSVQPCVLCTFTLQW*AETICDH
SLASILKGEILFSRFSPK*TLLYF***PRLFKL
LDLIGKLVIAKL*ITREESTF*KSLCFPSSKYY
IQYL*SVSCK*AQNTFKIQRRFFQAVIMVNITKP
SSHQGR*CDK*THHSEANYLMEYKSNGHPYFLI
LAFISLSFGWLVNGEELSG*FNQLQTICPCPKM
MSQISIKPVLVSPS*YCSLRHSLSLILRSCFKRH
NHFELP*NLSSTTTTLGEKNMLKEVSWLNNSL*
PISTVAISTQRKGPKSLCSLKISVNLKEQKQDR
TSGTLIRMIPNKSVICELSGLFGYFNLHIFSSYI
GLYLWPCSSFQC*SAKQKLLLMMCE*TYATAWP
FFLQSLLSFSLY*TLQYAQKLSFIK*KEKNIWL
IFHCS*SFIQ*SCKKIS*ILNITLSRFLKSKSFQ
*KVSYFILLY*VVKNVGLFTITCSLISEIYNSI
LRP**DSSIPCSTYGVL*ELIVGDELLLILFSS
FHCWFIADLYPVSNSRYY**TFSTSSKKFKFFSV
TVTENTICI*HLCSNKCAIFFLTQ*

Frame 1

PEASRARW*GRLSEKNVRLKRPTSSIRGDRQPA
PGGRGGQARKPPPPPRPESSCGAK*TEPLGGAR
GSPSPFSRPK*TLIGVVGCGDGAAEVAEDESPAT
THGRYRDRQGAERPTGLQCDVFPTC**WEN*TN
YTGKQSIQWIRY**KRRYNILGNKWSTKSFL*F
I*RFYRQPSLQ*SLE*QSIRSP*RLVPNTFHELK
SDGKNIRERLIFPVQQRYWITRLSI*SPETRSG
AWEPSTAIFFRKTWDSILFILCYKFQEETTP*L
CSA*SLASKKSQKGASWFAFFCICTIPKFR*FQP
*IS*LSIS*ATNQYVR*HFLYARWDPQFF*PLE
FIKWDEPAWFWWNSGDLHFPHVSIQ*LWQPSFT
*PLELSSTLSFTNRHKHESSTNVQLSSRQYQQFT
LRCCLTHSSHQWIRQHSRNQRECCWKLTDR*CT
WKGFGIYLFS*PYQQ*FSVKSINTSWITFTSHR
YQSVAKTWRASTFIPKL*KLTPLPEKSS*AATSR
AFARCNVLLKGCL*AVSNGGSFRQTG*CNPCAA
EPCCGTFHQFACWSQ*YT*FIGTIP*CTNWKPQ
FKLWRIKPCCKQSISFNGWNSSGRLCQSQWQSFS
PV*YSHYFKHRPEP*NTRKL*RWLAKSVWNCCY
NRNQD*KQRKG*KPS*TSFIR*HEVR**ILPKR
YQGFI*RQNKQY**R*GFEP*TEDRKGEGEADG*
QCQRTLTRAGY**SIQRAWPNVSASLEE*KTPN
KTPYSSSSRGSHP*SRTASQREEP*PQSSLP*E
KGRRKSFCRIGRAANHTARNPSWA**NYQPYGSY
VNISQFQSYQ*AR*KVTSPHKDLDNSDYLKTQT
*QEGEEKTKHLNQETQM*SYDQSNWSTLPSEVK
IGSSSDRTSAHEMFATNLLLQAVCRFCTIRDCLD
LSTHRGSCLVPKLN*QMHCNYKFYLLL*NCKVY
I*REKVNVES*STLS*CQHTLKRFTCREQSSDN
HWPLAFPAYLLVS*KGREKSFVALSYPLAI*IAF
SMK*ENITWYSISLALIF*FIFIFSLWVLYLIS
KSEQFMVTVQPPPCSPVCFAHLPYSGKQRPSVT
IA*LAF*KGKFCSLGFPPNKHCFISNNNQDFSSF
*IS*ESL**QNCKLQGKNLLFRNRFVFQAVSTT
YSTCKVLAVSKHKIHLKYKDDFFRL*LW*T*QN
PVVTKAGSVINEHTTLRLIT*WNTRAMVTRISLS
*PLFLCHLDGWSMGKN*VGDLINCKPSAPVPK*
*ARLALNQYLSVHLNTVH*GTLCL*SLGVVLKD
IITLNFHETCLPPQQPWERKTC*RRYLGLIIPYS
QYQQWQSAHRGKDPNHYVA*RFLLI*KNKNKTE
LLVL*SG*FLTSQSFVNLVDFLVTLICIYSPVT
SDSICGLVLHFSVNQLNRSCCL*CVSEHMPLPGL
FFFRACCLFRYIRLCSMPRSFPS*NRKKKTFGL
FFTVASLLYNNLVRKFLEF*ILLFLDF*NQKVF
SKKFLTLFYYIR**KM*GYLP*PVH*YQKFTIAF
*DHSRILAYRVVPMEYCKS*LLEMNCFSSCSPV
SIVGLLQICILCQIQGIIDKPFQPAARSSNFFL
SL*QKTQYVYNIYVAINVPSFF*HSK

Frame 2

RRRAERGGEAASAKKMSA*RDPQVLFGGTDSPP
REEGAARPESRLPLPDPRARAGQSEPSRWAVQG
EAQARSPGQSEL*SGWLDAETGRQKWPKMNPQQ
QRMAAIGTDKELSDLLDFSAMFSPPVNSGKTRPT
TLGSSQFSGSGIDERGGTTSWGTSGQPSPSYDS
SRGFTDSPHYSDHLNDSRLGAHEGLSPTPFMNS
NLMGKTSERGSFSLYSRDTGLPGCQSSLLRQDLG
LGSPAQLSSSGKPGTAYYSFSATSSRRRPLHDS
AALDPLQAKKVRKVPPGLPSSVYAPSPNSDDFN
RESPSYPSPKPPTSMFASTFFMQDGTHNSSDLWS
SSNGMSQPGFGGILGTSTSHMSQSSSYGNLHSH
DRLSYPPHSVSPTDINTSLPPMSSFHRGSTSSS
PYVAASHTPPINGSDSILGTRGNAAGSSQTGDAL
GKALASIYSPDHTSSSFPSNPSTPVGSPSPLTG
TSQWPRPGGQAPSSPSYENSLHSLKNRVEQQLH
EHLQDAMSFLKDVCEQSRMEDRLDRLDDAIHVLR
NHAVGPSTSLPAGHSDIHSLLGPSHNAPIGSLN
SNYGGSSLVASSRSASMVGTHREDSVSLNGNHS
VLSSTVTTSSTDLNHKTQENYRGGLQSQSGTVVT
TEIKTENKEKDENLHEPPSSDDMKSDDESSQKD
IKVSSRGRTSSTNEDEDLNPEQKIEREKERRMA
NNARERLRVRDINEAFKELGRMCQLHLKSEKPQT
KLLILHQAVAVILSLEQQVRERNLNPKAACLKR
REEEKVSAVSAEPPTTLPGTHPGLSETTNPMGH
M*TSASSRVISRLDRR*PLLIRTWTTQII*RHKP
DRREKKKQNT*TKKLKCNPTIKATGQHFHQK*R
*EAHQIEHQPMRCLQQIFCCKQCVASAQSETVS
ISPLTVEVALCLN*IDKCIVTTNFIYCYETVRST
YKGKKLMWKADLHSADASIH*SGSRAENKAVTT
IGP*HSRHTY*CLKKEGKSLLLPSPILLPYE*R
FP*NRKILLGIAFLLLSFFDLFLFSLCGCYI*SL
NLNSLWSQSSLLRAALCALHIYLTVVSRDHL*P
*PS*HFKRGNFVL*VFPQINIALFLIITKTFQA
SRSHRKACNSKIVNYKGRIYFLEIALFSKQ*VLH
TVLVKC*L*VSTKYI*NTKTIFSGCDYGEHNKT
Q*SPRQVV**MNTPL*G*LPNGIQEQWSPVFPY
PSLYFSVIWMAGQWGRIEWVI*STANHLPLSQND
EPD*H*TSTCQSILILFIKALSVSNP*ELF*KT
*SL*TSMKPVFHHNNPGREKHAKGGILA**FLI
ANINSGNQHTEERTQITM*LKDFC*FERTKTRQN
FWYSNQDDS*QVSHL*T*WTFWLL*FAYILQLH
RTLSVALFFISVLIS*TEVVAYDV*VNICHCLA
FFSSELVVFFAILDFAVCPEAFLHKIERKKHLAY
FSL*LVFYTIIL*ENFLNSKYYSF*IFEIKKFS
VKSFLLYFIILGSKKCRVIYHNLFINIRNLQ*H
FKTIVGF*HTV*YLWSIVRANCWR*IASHLVLQF
PLLVYCRFVSCVKFKVLLINLFNQQQEVQIFFC
HCNRKHNMYITFM*Q*MCHLFFNTVK

Sequence NM_207037

TCCGGAGGCGAGCCGAGCGCGGTGGTGAGGCCGCCTCAGCGAAAAAAATGTCCGCCTGAAGAGACCCACAAGTTCTATTCGGGGGGACCGACAGCCCGCC
CCGGGAGGAAGGGGCGGCCAGGCCCGAAAGCCGCCTCCCCCTCCCAGACCCGAGAGCTCGTGCGGGGCAAAGTGAACCGAGCCGCTGGGCGGTGCAAGGG
GAAGCCCAAGCCCGTTCTCCCGGCCAAAGTGAACTTTAATCGGGGTGGTTGGATGCGGAGACGGGGCGGCAGGACCTGCTAGAAGTGGCCGAAGATGAAT
CCCCAGCAACAACGCATGGCCGCTATAGGGACCGACAAGGAGCTGAGCGACCTACTGGACTTCAGTGCGATGTTTTCCCCACCTGTTAATAGTGGGAAAA
CTAGACCAACTACACTGGGAAGCAGTCAATTCAGTGGATCAGGTATTGATGAAAGAGGAGGTACAACATCTTGGGGAACAAGTGGTCAACCAAGTCCTTC
CTATGATTCATCTAGAGGTTTTACAGACAGCCCTCATTACAGTGATCACTTGAATGACAGTCGATTAGGAGCCCATGAAGGCTTGTCCCCAACACCTTTC
ATGAACTCAAATCTGATGGGAAAAACATCAGAGAGAGGCTCATTTTCCCTGTACAGCAGAGATACTGGATTACCAGGCTGTCAATCTAGTCTCCTGAGAC
AAGATCTGGGGCTTGGGAGCCCAGCACAGCTATCTTCTTCAGGAAAACCTGGGACAGCATACTATTCATTCTCTGCTACAAGTTCCAGGAGGAGACCACT
CCATGACTCTGCAGCGCTTGATCCCTTGCAAGCAAAAAAAGTCAGAAAGGTGCCTCCTGGTTTGCCTTCTTCTGTATATGCACCATCCCCAAATTCAGAT
GATTTCAACCGTGAATCTCCTAGTTATCCATCTCCTAAGCCACCAACCAGTATGTTCGCTAGCACTTTCTTTATGCAAGATGGGACCCACAATTCTTCTG
ACCTTTGGAGTTCATCAAATGGGATGAGCCAGCCTGGTTTTGGTGGAATTCTGGGGACCTCCACTTCCCACATGTCTCAATCCAGTAGTTATGGCAACCT
TCATTCACATGACCGCTTGAGTTATCCTCCACACTCAGTTTCACCAACAGACATAAACACGAGTCTTCCACCAATGTCCAGCTTTCATCGCGGCAGTACC
AGCAGTTCACCTTACGTTGCTGCCTCACACACTCCTCCCATCAATGGATCAGACAGCATTCTAGGAACCAGAGGGAATGCTGCTGGAAGCTCACAGACAG
GTGATGCACTTGGAAAGGCTTTGGCATCTATTTATTCTCCTGACCATACCAGCAGTAGTTTTCCGTCAAATCCATCAACACCAGTTGGATCACCTTCACC
TCTCACAGGTACCAGTCAGTGGCCAAGACCTGGAGGGCAAGCACCTTCATCCCCAAGCTATGAAAACTCACTCCACTCCCTGAAAAATCGAGTTGAGCAG
CAACTTCACGAGCATTTGCAAGATGCAATGTCCTTCTTAAAGGATGTCTGTGAGCAGTCTCGAATGGAGGATCGTTTAGACAGACTGGATGATGCAATCC
ATGTGCTGCGGAACCATGCTGTGGGACCTTCCACCAGTTTGCCTGCTGGTCACAGTGATATACATAGTTTATTGGGACCATCCCATAATGCACCAATTGG
AAGCCTCAATTCAAACTATGGAGGATCAAGCCTTGTTGCAAGCAGTCGATCAGCTTCAATGGTTGGAACTCATCGGGAAGACTCTGTCAGTCTCAATGGC
AATCATTCAGTCCTGTCTAGTACAGTCACTACTTCAAGCACAGACCTGAACCATAAAACACAAGAAAATTATAGAGGTGGCTTGCAAAGTCAGTCTGGAA
CTGTTGTTACAACAGAAATCAAGACTGAAAACAAAGAAAAGGATGAAAACCTTCATGAACCTCCTTCATCAGATGACATGAAGTCAGATGATGAATCCTC
CCAAAAAGATATCAAGGTTTCATCTAGAGGCAGAACAAGCAGTACTAATGAAGATGAGGATTTGAACCCTGAACAGAAGATAGAAAGGGAGAAGGAGAGG
CGGATGGCTAACAATGCCAGAGAACGCTTACGCGTGCGGGATATTAATGAAGCATTCAAAGAGCTTGGCCGAATGTGTCAGCTTCACTTGAAGAGTGAAA
AACCCCAAACAAAACTCCTTATTCTTCATCAAGCCGTGGCAGTCATCCTTAGTCTAGAACAGCAAGTCAGAGAGAGGAACCTTAACCCCAAAGCAGCCTG
CCTTAAGAGAAGGGAAGAAGAAAAAGTTTCTGCCGTATCGGCAGAGCCGCCAACCACACTGCCAGGAACCCATCCTGGGCTTAGTGAAACTACCAACCCT
ATGGGTCATATGTAAACATCAGCCAGTTCCAGAGTTATCAGTAGGCTAGATAGAAGGTGACCTCTCCTCATAAGGACTTGGACAACTCAGATTATCTGAA
GACACAAACCTGACAGGAGGGAGAAGAAAAAACAAAACACTTGAACCAAGAAACTCAAATGTAATCCTACGATCAAAGCAACTGGTCAACACTTCCATCA
GAAGTGAAGATAGGAAGCTCATCAGATAGAACATCAGCCCATGAGATGTTTGCAACAAATCTTTTGTTGCAAGCAGTGTGTCGCTTCTGCACAATCAGAG
ACTGTCTCGATCTCTCCACTCACCGTGGAAGTTGCCTTGTGCCTAAACTGAATTGACAAATGCATTGTAACTACAAATTTTATTTATTGTTATGAAACTG
TAAGGTCTACATATAAAGGGAAAAAGTTAATGTGGAAAGCTGATCTACACTCAGCTGATGCCAGCATACATTAAAGCGGTTCACGTGCAGAGAACAAAGC
AGTGACAACCATTGGCCCTTAGCATTCCCGGCATACCTATTAGTGTCTTAAAAAGGAAGGGAAAAGTCTTTTGTTGCCCTCTCCTATCCTCTTGCCATAT
GAATAGCGTTTTCCATGAAATAGGAAAATATTACTTGGTATAGCATTTCTCTTGCTCTCATTTTTTGATTTATTTTTATTTTCTCTTTGTGGGTGTTATA
TTTGATCTCTAAATCTGAACAGTTTATGGTCACAGTCCAGCCTCCTCCGTGCAGCCCTGTGTGCTTTGCACATTTACCTTACAGTGGTAAGCAGAGACCA
TCTGTGACCATAGCCTAGCTAGCATTTTAAAAGGGGAAATTTTGTTCTCTAGGTTTTCCCCCAAATAAACATTGCTTTATTTCTAATAATAACCAAGACT
TTTCAAGCTTCTAGATCTCATAGGAAAGCTTGTAATAGCAAAATTGTAAATTACAAGGGAAGAATCTACTTTTTAGAAATCGCTTTGTTTTCCAAGCAGT
AAGTACTACATACAGTACTTGTAAAGTGTTAGCTGTAAGTAAGCACAAAATACATTTAAAATACAAAGACGATTTTTTCAGGCTGTGATTATGGTGAACA
TAACAAAACCCAGTAGTCACCAAGGCAGGTAGTGTGATAAATGAACACACCACTCTGAGGCTAATTACCTAATGGAATACAAGAGCAATGGTCACCCGTA
TTTCCTTATCCTAGCCTTTATTTCTCTGTCATTTGGATGGCTGGTCAATGGGGAAGAATTGAGTGGGTGATTTAATCAACTGCAAACCATCTGCCCCTGT
CCCAAAATGATGAGCCAGATTAGCATTAAACCAGTACTTGTCAGTCCATCTTAATACTGTTCATTAAGGCACTCTCTGTCTCTAATCCTTAGGAGTTGTT
TTAAAAGACATAATCACTTTGAACTTCCATGAAACCTGTCTTCCACCACAACAACCCTGGGAGAGAAAAACATGCTAAAGGAGGTATCTTGGCTTAATAA
TTCCTTATAGCCAATATCAACAGTGGCAATCAGCACACAGAGGAAAGGACCCAAATCACTATGTAGCTTAAAGATTTCTGTTAATTTGAAAGAACAAAAA
CAAGACAGAACTTCTGGTACTCTAATCAGGATGATTCCTAACAAGTCAGTCATTTGTGAACTTAGTGGACTTTTTGGTTACTTTAATTTGCATATATTCT
CCAGTTACATCGGACTCTATCTGTGGCCTTGTTCTTCATTTCAGTGTTAATCAGCTAAACAGAAGTTGTTGCTTATGATGTGTGAGTGAACATATGCCAC
TGCCTGGCCTTTTTTTCTTCAGAGCTTGTTGTCTTTTTCGCTATATTAGACTTTGCAGTATGCCCAGAAGCTTTCCTTCATAAAATAGAAAGAAAAAAAC
ATTTGGCTTATTTTTCACTGTAGCTAGTCTTTTATACAATAATCTTGTAAGAAAATTTCTTGAATTCTAAATATTACTCTTTCTAGATTTTTGAAATCAA
AAAGTTTTCAGTAAAAAGTTTCTTACTTTATTTTATTATATTAGGTAGTAAAAAATGTAGGGTTATTTACCATAACCTGTTCATTAATATCAGAAATTTA
CAATAGCATTTTAAGACCATAGTAGGATTCTAGCATACCGTGTAGTACCTATGGAGTATTGTAAGAGCTAATTGTTGGAGATGAATTGCTTCTCATCTTG
TTCTCCAGTTTCCATTGTTGGTTTATTGCAGATTTGTATCCTGTGTCAAATTCAAGGTATTATTGATAAACCTTTTCAACCAGCAGCAAGAAGTTCAAAT
TTTTTTCTGTCACTGTAACAGAAAACACAATATGTATATAACATTTATGTAGCAATAAATGTGCCATCTTTTTTTTAACACAGTAAAA
  • refseq_TCF12.F2 (forward primer)
  • refseq_TCF12.R2 (reverse primer)
  • Single exon skipping

Frame 0

SGGEPSAVVRPPQRKKCPPEETHKFYSGGPTAR
PGRKGRPGPKAASPSQTRELVRGKVNRAAGRCKG
KPKPVLPAKVNFNRGGWMRRRGGRTC*KWPKMN
PQQQRMAAIGTDKELSDLLDFSAMFSPPVNSGK
TRPTTLGSSQFSGSGIDERGGTTSWGTSGQPSPS
YDSSRGFTDSPHYSDHLNDSRLGAHEGLSPTPF
MNSNLMGKTSERGSFSLYSRDTGLPGCQSSLLR
QDLGLGSPAQLSSSGKPGTAYYSFSATSSRRRPL
HDSAALDPLQAKKVRKVPPGLPSSVYAPSPNSD
DFNRESPSYPSPKPPTSMFASTFFMQDGTHNSS
DLWSSSNGMSQPGFGGILGTSTSHMSQSSSYGNL
HSHDRLSYPPHSVSPTDINTSLPPMSSFHRGST
SSSPYVAASHTPPINGSDSILGTRGNAAGSSQT
GDALGKALASIYSPDHTSSSFPSNPSTPVGSPSP
LTGTSQWPRPGGQAPSSPSYENSLHSLKNRVEQ
QLHEHLQDAMSFLKDVCEQSRMEDRLDRLDDAI
HVLRNHAVGPSTSLPAGHSDIHSLLGPSHNAPIG
SLNSNYGGSSLVASSRSASMVGTHREDSVSLNG
NHSVLSSTVTTSSTDLNHKTQENYRGGLQSQSG
TVVTTEIKTENKEKDENLHEPPSSDDMKSDDESS
QKDIKVSSRGRTSSTNEDEDLNPEQKIEREKER
RMANNARERLRVRDINEAFKELGRMCQLHLKSE
KPQTKLLILHQAVAVILSLEQQVRERNLNPKAAC
LKRREEEKVSAVSAEPPTTLPGTHPGLSETTNP
MGHM*TSASSRVISRLDRR*PLLIRTWTTQII*
RHKPDRREKKKQNT*TKKLKCNPTIKATGQHFHQ
K*R*EAHQIEHQPMRCLQQIFCCKQCVASAQSE
TVSISPLTVEVALCLN*IDKCIVTTNFIYCYET
VRSTYKGKKLMWKADLHSADASIH*SGSRAENKA
VTTIGP*HSRHTY*CLKKEGKSLLLPSPILLPY
E*RFP*NRKILLGIAFLLLSFFDLFLFSLCGCY
I*SLNLNSLWSQSSLLRAALCALHIYLTVVSRDH
L*P*PS*HFKRGNFVL*VFPQINIALFLIITKT
FQASRSHRKACNSKIVNYKGRIYFLEIALFSKQ
*VLHTVLVKC*L*VSTKYI*NTKTIFSGCDYGEH
NKTQ*SPRQVV**MNTPL*G*LPNGIQEQWSPV
FPYPSLYFSVIWMAGQWGRIEWVI*STANHLPL
SQNDEPD*H*TSTCQSILILFIKALSVSNP*ELF
*KT*SL*TSMKPVFHHNNPGREKHAKGGILA**
FLIANINSGNQHTEERTQITM*LKDFC*FERTK
TRQNFWYSNQDDS*QVSHL*T*WTFWLL*FAYIL
QLHRTLSVALFFISVLIS*TEVVAYDV*VNICH
CLAFFSSELVVFFAILDFAVCPEAFLHKIERKK
HLAYFSL*LVFYTIIL*ENFLNSKYYSF*IFEIK
KFSVKSFLLYFIILGSKKCRVIYHNLFINIRNL
Q*HFKTIVGF*HTV*YLWSIVRANCWR*IASHL
VLQFPLLVYCRFVSCVKFKVLLINLFNQQQEVQI
FFCHCNRKHNMYITFM*Q*MCHLFFNTVK

Frame 1

PEASRARW*GRLSEKNVRLKRPTSSIRGDRQPA
PGGRGGQARKPPPPPRPESSCGAK*TEPLGGAR
GSPSPFSRPK*TLIGVVGCGDGAAGPARSGRR*I
PSNNAWPL*GPTRS*ATYWTSVRCFPHLLIVGK
LDQLHWEAVNSVDQVLMKEEVQHLGEQVVNQVL
PMIHLEVLQTALITVIT*MTVD*EPMKACPQHLS
*TQI*WEKHQREAHFPCTAEILDYQAVNLVS*D
KIWGLGAQHSYLLQENLGQHTIHSLLQVPGGDH
SMTLQRLIPCKQKKSERCLLVCLLLYMHHPQIQM
ISTVNLLVIHLLSHQPVCSLALSLCKMGPTILL
TFGVHQMG*ASLVLVEFWGPPLPTCLNPVVMAT
FIHMTA*VILHTQFHQQT*TRVFHQCPAFIAAVP
AVHLTLLPHTLLPSMDQTAF*EPEGMLLEAHRQ
VMHLERLWHLFILLTIPAVVFRQIHQHQLDHLH
LSQVPVSGQDLEGKHLHPQAMKTHSTP*KIELSS
NFTSICKMQCPS*RMSVSSLEWRIV*TDWMMQS
MCCGTMLWDLPPVCLLVTVIYIVYWDHPIMHQL
EASIQTMEDQALLQAVDQLQWLELIGKTLSVSMA
IIQSCLVQSLLQAQT*TIKHKKIIEVACKVSLE
LLLQQKSRLKTKKRMKTFMNLLHQMT*SQMMNP
PKKISRFHLEAEQAVLMKMRI*TLNRR*KGRRRG
GWLTMPENAYACGILMKHSKSLAECVSFT*RVK
NPKQNSLFFIKPWQSSLV*NSKSERGTLTPKQP
ALREGKKKKFLPYRQSRQPHCQEPILGLVKLPTL
WVICKHQPVPELSVG*IEGDLSS*GLGQLRLSE
DTNLTGGRRKNKTLEPRNSNVILRSKQLVNTSI
RSEDRKLIR*NISP*DVCNKSFVASSVSLLHNQR
LSRSLHSPWKLPCA*TELTNAL*LQILFIVMKL
*GLHIKGKS*CGKLIYTQLMPAYIKAVHVQRTK
Q*QPLALSIPGIPISVLKRKGKVFCCPLLSSCHM
NSVFHEIGKYYLV*HFSCSHFLIYFYFLFVGVI
FDL*I*TVYGHSPASSVQPCVLCTFTLQW*AET
ICDHSLASILKGEILFSRFSPK*TLLYF***PRL
FKLLDLIGKLVIAKL*ITREESTF*KSLCFPSS
KYYIQYL*SVSCK*AQNTFKIQRRFFQAVIMVN
ITKPSSHQGR*CDK*THHSEANYLMEYKSNGHPY
FLILAFISLSFGWLVNGEELSG*FNQLQTICPC
PKMMSQISIKPVLVSPS*YCSLRHSLSLILRSC
FKRHNHFELP*NLSSTTTTLGEKNMLKEVSWLNN
SL*PISTVAISTQRKGPKSLCSLKISVNLKEQK
QDRTSGTLIRMIPNKSVICELSGLFGYFNLHIF
SSYIGLYLWPCSSFQC*SAKQKLLLMMCE*TYAT
AWPFFLQSLLSFSLY*TLQYAQKLSFIK*KEKN
IWLIFHCS*SFIQ*SCKKIS*ILNITLSRFLKS
KSFQ*KVSYFILLY*VVKNVGLFTITCSLISEIY
NSILRP**DSSIPCSTYGVL*ELIVGDELLLIL
FSSFHCWFIADLYPVSNSRYY**TFSTSSKKFK
FFSVTVTENTICI*HLCSNKCAIFFLTQ*

Frame 2

RRRAERGGEAASAKKMSA*RDPQVLFGGTDSPP
REEGAARPESRLPLPDPRARAGQSEPSRWAVQG
EAQARSPGQSEL*SGWLDAETGRQDLLEVAEDE
SPATTHGRYRDRQGAERPTGLQCDVFPTC**WEN
*TNYTGKQSIQWIRY**KRRYNILGNKWSTKSF
L*FI*RFYRQPSLQ*SLE*QSIRSP*RLVPNTF
HELKSDGKNIRERLIFPVQQRYWITRLSI*SPET
RSGAWEPSTAIFFRKTWDSILFILCYKFQEETT
P*LCSA*SLASKKSQKGASWFAFFCICTIPKFR
*FQP*IS*LSIS*ATNQYVR*HFLYARWDPQFF*
PLEFIKWDEPAWFWWNSGDLHFPHVSIQ*LWQP
SFT*PLELSSTLSFTNRHKHESSTNVQLSSRQY
QQFTLRCCLTHSSHQWIRQHSRNQRECCWKLTDR
*CTWKGFGIYLFS*PYQQ*FSVKSINTSWITFT
SHRYQSVAKTWRASTFIPKL*KLTPLPEKSS*A
ATSRAFARCNVLLKGCL*AVSNGGSFRQTG*CNP
CAAEPCCGTFHQFACWSQ*YT*FIGTIP*CTNW
KPQFKLWRIKPCCKQSISFNGWNSSGRLCQSQW
QSFSPV*YSHYFKHRPEP*NTRKL*RWLAKSVWN
CCYNRNQD*KQRKG*KPS*TSFIR*HEVR**IL
PKRYQGFI*RQNKQY**R*GFEP*TEDRKGEGE
ADG*QCQRTLTRAGY**SIQRAWPNVSASLEE*K
TPNKTPYSSSSRGSHP*SRTASQREEP*PQSSL
P*EKGRRKSFCRIGRAANHTARNPSWA**NYQP
YGSYVNISQFQSYQ*AR*KVTSPHKDLDNSDYLK
TQT*QEGEEKTKHLNQETQM*SYDQSNWSTLPS
EVKIGSSSDRTSAHEMFATNLLLQAVCRFCTIR
DCLDLSTHRGSCLVPKLN*QMHCNYKFYLLL*NC
KVYI*REKVNVES*STLS*CQHTLKRFTCREQS
SDNHWPLAFPAYLLVS*KGREKSFVALSYPLAI
*IAFSMK*ENITWYSISLALIF*FIFIFSLWVLY
LISKSEQFMVTVQPPPCSPVCFAHLPYSGKQRP
SVTIA*LAF*KGKFCSLGFPPNKHCFISNNNQD
FSSF*IS*ESL**QNCKLQGKNLLFRNRFVFQAV
STTYSTCKVLAVSKHKIHLKYKDDFFRL*LW*T
*QNPVVTKAGSVINEHTTLRLIT*WNTRAMVTR
ISLS*PLFLCHLDGWSMGKN*VGDLINCKPSAPV
PK**ARLALNQYLSVHLNTVH*GTLCL*SLGVV
LKDIITLNFHETCLPPQQPWERKTC*RRYLGLI
IPYSQYQQWQSAHRGKDPNHYVA*RFLLI*KNKN
KTELLVL*SG*FLTSQSFVNLVDFLVTLICIYS
PVTSDSICGLVLHFSVNQLNRSCCL*CVSEHMP
LPGLFFFRACCLFRYIRLCSMPRSFPS*NRKKKT
FGLFFTVASLLYNNLVRKFLEF*ILLFLDF*NQ
KVFSKKFLTLFYYIR**KM*GYLP*PVH*YQKF
TIAF*DHSRILAYRVVPMEYCKS*LLEMNCFSSC
SPVSIVGLLQICILCQIQGIIDKPFQPAARSSN
FFLSL*QKTQYVYNIYVAINVPSFF*HSK

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