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Sequences

Sequence NM_201430

refseq_RTN3.F2 (forward primer)
refseq_RTN3.R2 (reverse primer)
Single exon skipping

Frame 0

ACALALPPSSCARLSQSVCRSLSSEQAE*RDLS EPVAGLFYFPSLSPAPYLFSPFSHPRSRSHGGAV GGHSVPFHLLVVLRSRAVRARRRREPRSLPRPG DEELQLLLCGARSDFLERCEEDWVCLWHHADHA AFPGSFQCHQCGFLPHPGSSLCHHQLQDLQVRHP SCTEVRRRPSIQSLPGRRHYSVLRSFP*LHECC HGAHQQGPETHYSSLSGRRSG*LLEAGCLHVAD DLCWCCF*RNHPSNSC*TAHFQCPDCL*EVQGSK QNSLESPKKRQNKYMETRNATVTKTPFNSYNVV TCTMKENTQCQLEPAFQAFFLIWCFLPSFPFNP QYQAQKLMD**KNYLRTQKKKESNS*DKSIP*WW *SLYL*LERGKIGGKRENERTPLGPSVQFSALV LLSYPL*FCP*EVMINL*KNYLGTGV*YLPWFF FAALKSYLPAPQCEQLPLILLFFNDLTINLINNL *VIVIIPDSKNAI**KRIEMESGTERESAGMLR WRSLPLPLSPGKERLRHLGKWFLRHWTPVLSIS LRTRSRMCFPPSPLPTSSLINKVVLFLL*NKCL* LLCTAVNLLEKKITCMWAPQLLSFCDPISVWGG TFSRGEIQKAFFS*SFPEIQISDSHLGASFFLH LQYENSANLKEKSSVSSFRFSLIVMILQREYALA SLK*GL*YLMVPKYGSCKK*WKEIVYVSWKN*L GIWMGKRYPCLTPSYFLSPL*VF*LVSCPSRKC ILHPHPLPSQEVSDCLFGLPLQRTFCSGSATSSS FLLLLLCLDN*RDLCATQDFFFFLRKTYR*KIT NETVCTCLCVQHKNTVAPKELESWFL*NFKLMW Y**KKKKHKQ

Frame 1

HARSRSRPLAALG*VSQSVGVCPRSRRSKGT*A SQLPDYSISPPSLPPRISFHPSPTLARVAMAEP SAATQSHSISSSSFGAEPSAPGGGGSPGACPALG TKSCSSSCAVHDLIFWRDVKKTGFVFGTTLIML LSLAAFSVISVVSYLILALLSVTISFRIYKSVI QAVQKSEEGHPFKAYLDVDITLSSEAFHNYMNAA MVHINRALKLIIRLFLVEDLVDSLKLAVFMWLM TYVGAVFNGITLLILAELLIFSVPIVYEKYKDP SKTPWNRQKKGRISTWKPEMQQLLKHHLIVITSL LVL*RKILSVSLSLHSKLFF*FGVFSHPFPLTL SIKHKN*WTDKRTILELRRRKNQIHRISQYLNG GRAFTCSLKGERLEVREKMKEHLWVLLSSFQH*S YSAIHYSFALKKS*LTYEKIIWGQECDTFLGFF LQPSNPIFLPHNVSSYP*YSFSLMI*LST**IT YR****FLIPRMPSDKKE*KWKVGLRGSQQACCG GGHSLCHYPQGRKGSAIWESGFYVTGHRF*ALV *ELVPECAFLPLPCPPQV**IRLYFSYYKINVC NCCALL*TC*RKK*PACGLLSY*VFVILSQSGGE HSQEVKYRKPFFLDLFPRFKSPIPIWGQVFFFT FNMRIQRT*KKNHL*VPSGSHS*S*SFRGNMHW RV*SKGYDI*WSQSTAAAKSSGRKLSTCLGKIS* EFGWVKGTLALLHLIFLAPFECFNWFHVLVGSA FSILILCPPRKSVIVFLGFPSKGPSAVEVPHPV LFFCCCCV*IIEEIFVPHRIFFFF*EKPIDEKLL MKLCVRVCACNIKIQ*HLRSLNLGSCKISN*CG INKKKKNTN

Frame 2

MRARAPAL*LRSAESVSLSESVLGAGGVKGLER ASCRIILFPLPLSRPVSLFTLLPPSLA*PWRSR RRPLSPIPSPRRPSEPSRPRPAAAGAQEPAPPW GRRAAAPPVRCTI*FSGEM*RRLGLSLAPR*SCC FPWQLSVSSVWFLTSSWLFSLSPSASGSTSPSS KLYRSQKKAIHSKPTWT*TLLCPQKLSIIT*ML PWCTSTGP*NSLFVSFW*KIWLTP*SWLSSCG** PMLVLFLTESPF*FLLNCSFSVSRLSMRSTRIQ AKLPGIAKKKAE*VHGNQKCNSY*NTI**L*RR YLYYEGKYSVSA*ACIPSFFFNLVFSPILSL*PS VSSTKIDGLIKELS*NSEEERIKFIG*VNTLMV VEPLPVA*KGKDWR*ERK*KNTSGSFCPVFSTS LTQLSIIVLPLRSHD*LMKKLFGDRSVIPSLVFF CSPQILSSCPTM*AATPDTPFL**FNYQLDK*L IGDSDNS*FQECHLIKKNRNGKWD*EGVSRHAA VAVTPSATIPREGKAPPFGKVVSTSLDTGSEH*F ENSFPNVLSSLSPAHLKFNK*GCTFLTIK*MSV TAVHCCKLVREKNNLHVGSSVIEFL*SYLSLGG NILKR*NTESLFFLIFSRDSNLRFPFGGKFFSSP SI*EFSELERKIICEFLQVLTHSHDPSEGICTG EFKVRAMIFDGPKVRQLQKVVEGNCLRVLEKLV RNLDG*KVPLPYSILFS*PPLSVLTGFMS**EVH SPSSSSALPGSQ*LSFWASPPKDLLQWKCHIQF FSFVAAVFR*LKRSLCHTGFFFFFKKNL*MKNY **NCVYVSVRAT*KYSST*GA*ILVPVKFQIDVV LIKKKKTQT

Sequence NM_006054

refseq_RTN3.F2 (forward primer)
refseq_RTN3.R2 (reverse primer)
Single exon skipping

Frame 0

ACALALPPSSCARLSQSVCRSLSSEQAE*RDLS EPVAGLFYFPSLSPAPYLFSPFSHPRSRSHGGAV GGHSVPFHLLVVLRSRAVRARRRREPRSLPRPG DEELQLLLCGARSDFLERCEEDWVCLWHHADHA AFPGSFQCHQCGFLPHPGSSLCHHQLQDLQVRHP SCTEVRRRPSIQSLPGRRHYSVLRSFP*LHECC HGAHQQGPETHYSSLSGRRSG*LLEAGCLHVAD DLCWCCF*RNHPSNSC*TAHFQCPDCL*EVQDPD *SLCWHRPRSDQVNC*KDPSKTPWNRQKKGRIS TWKPEMQQLLKHHLIVITSLLVL*RKILSVSLS LHSKLFF*FGVFSHPFPLTLSIKHKN*WTDKRTI LELRRRKNQIHRISQYLNGGRAFTCSLKGERLE VREKMKEHLWVLLSSFQH*SYSAIHYSFALKKS *LTYEKIIWGQECDTFLGFFLQPSNPIFLPHNVS SYP*YSFSLMI*LST**ITYR****FLIPRMPS DKKE*KWKVGLRGSQQACCGGGHSLCHYPQGRK GSAIWESGFYVTGHRF*ALV*ELVPECAFLPLPC PPQV**IRLYFSYYKINVCNCCALL*TC*RKK* PACGLLSY*VFVILSQSGGEHSQEVKYRKPFFL DLFPRFKSPIPIWGQVFFFTFNMRIQRT*KKNHL *VPSGSHS*S*SFRGNMHWRV*SKGYDI*WSQS TAAAKSSGRKLSTCLGKIS*EFGWVKGTLALLH LIFLAPFECFNWFHVLVGSAFSILILCPPRKSVI VFLGFPSKGPSAVEVPHPVLFFCCCCV*IIEEI FVPHRIFFFF*EKPIDEKLLMKLCVRVCACNIK IQ*HLRSLNLGSCKISN*CGINKKKKNTN

Frame 1

HARSRSRPLAALG*VSQSVGVCPRSRRSKGT*A SQLPDYSISPPSLPPRISFHPSPTLARVAMAEP SAATQSHSISSSSFGAEPSAPGGGGSPGACPALG TKSCSSSCAVHDLIFWRDVKKTGFVFGTTLIML LSLAAFSVISVVSYLILALLSVTISFRIYKSVI QAVQKSEEGHPFKAYLDVDITLSSEAFHNYMNAA MVHINRALKLIIRLFLVEDLVDSLKLAVFMWLM TYVGAVFNGITLLILAELLIFSVPIVYEKYKTQ IDHYVGIARDQTKSIVEKIQAKLPGIAKKKAE*V HGNQKCNSY*NTI**L*RRYLYYEGKYSVSA*A CIPSFFFNLVFSPILSL*PSVSSTKIDGLIKEL S*NSEEERIKFIG*VNTLMVVEPLPVA*KGKDWR *ERK*KNTSGSFCPVFSTSLTQLSIIVLPLRSH D*LMKKLFGDRSVIPSLVFFCSPQILSSCPTM* AATPDTPFL**FNYQLDK*LIGDSDNS*FQECHL IKKNRNGKWD*EGVSRHAAVAVTPSATIPREGK APPFGKVVSTSLDTGSEH*FENSFPNVLSSLSP AHLKFNK*GCTFLTIK*MSVTAVHCCKLVREKNN LHVGSSVIEFL*SYLSLGGNILKR*NTESLFFL IFSRDSNLRFPFGGKFFSSPSI*EFSELERKII CEFLQVLTHSHDPSEGICTGEFKVRAMIFDGPKV RQLQKVVEGNCLRVLEKLVRNLDG*KVPLPYSI LFS*PPLSVLTGFMS**EVHSPSSSSALPGSQ* LSFWASPPKDLLQWKCHIQFFSFVAAVFR*LKRS LCHTGFFFFFKKNL*MKNY**NCVYVSVRAT*K YSST*GA*ILVPVKFQIDVVLIKKKKTQT

Frame 2

MRARAPAL*LRSAESVSLSESVLGAGGVKGLER ASCRIILFPLPLSRPVSLFTLLPPSLA*PWRSR RRPLSPIPSPRRPSEPSRPRPAAAGAQEPAPPW GRRAAAPPVRCTI*FSGEM*RRLGLSLAPR*SCC FPWQLSVSSVWFLTSSWLFSLSPSASGSTSPSS KLYRSQKKAIHSKPTWT*TLLCPQKLSIIT*ML PWCTSTGP*NSLFVSFW*KIWLTP*SWLSSCG** PMLVLFLTESPF*FLLNCSFSVSRLSMRSTRPR LITMLASPEIRPSQLLKRSKQNSLESPKKRQNK YMETRNATVTKTPFNSYNVVTCTMKENTQCQLEP AFQAFFLIWCFLPSFPFNPQYQAQKLMD**KNY LRTQKKKESNS*DKSIP*WW*SLYL*LERGKIG GKRENERTPLGPSVQFSALVLLSYPL*FCP*EVM INL*KNYLGTGV*YLPWFFFAALKSYLPAPQCE QLPLILLFFNDLTINLINNL*VIVIIPDSKNAI **KRIEMESGTERESAGMLRWRSLPLPLSPGKER LRHLGKWFLRHWTPVLSISLRTRSRMCFPPSPL PTSSLINKVVLFLL*NKCL*LLCTAVNLLEKKI TCMWAPQLLSFCDPISVWGGTFSRGEIQKAFFS* SFPEIQISDSHLGASFFLHLQYENSANLKEKSS VSSFRFSLIVMILQREYALASLK*GL*YLMVPK YGSCKK*WKEIVYVSWKN*LGIWMGKRYPCLTPS YFLSPL*VF*LVSCPSRKCILHPHPLPSQEVSD CLFGLPLQRTFCSGSATSSSFLLLLLCLDN*RD LCATQDFFFFLRKTYR*KITNETVCTCLCVQHKN TVAPKELESWFL*NFKLMWY**KKKKHKQ

Sequence NM_201429

refseq_RTN3.F2 (forward primer)
refseq_RTN3.R2 (reverse primer)
Single exon skipping

Frame 0

ACALALPPSSCARLSQSVCRSLSSEQAE*RDLS EPVAGLFYFPSLSPAPYLFSPFSHPRSRSHGGAV GGHSVPFHLLVVLRSRAVRARRRREPRSLPRPG DEELQLLLCGFLCFFLFLSACISIFDLTSARSD FLERCEEDWVCLWHHADHAAFPGSFQCHQCGFLP HPGSSLCHHQLQDLQVRHPSCTEVRRRPSIQSL PGRRHYSVLRSFP*LHECCHGAHQQGPETHYSS LSGRRSG*LLEAGCLHVADDLCWCCF*RNHPSNS C*TAHFQCPDCL*EVQDPD*SLCWHRPRSDQVN C*KDPSKTPWNRQKKGRISTWKPEMQQLLKHHL IVITSLLVL*RKILSVSLSLHSKLFF*FGVFSHP FPLTLSIKHKN*WTDKRTILELRRRKNQIHRIS QYLNGGRAFTCSLKGERLEVREKMKEHLWVLLS SFQH*SYSAIHYSFALKKS*LTYEKIIWGQECDT FLGFFLQPSNPIFLPHNVSSYP*YSFSLMI*LS T**ITYR****FLIPRMPSDKKE*KWKVGLRGS QQACCGGGHSLCHYPQGRKGSAIWESGFYVTGHR F*ALV*ELVPECAFLPLPCPPQV**IRLYFSYY KINVCNCCALL*TC*RKK*PACGLLSY*VFVIL SQSGGEHSQEVKYRKPFFLDLFPRFKSPIPIWGQ VFFFTFNMRIQRT*KKNHL*VPSGSHS*S*SFR GNMHWRV*SKGYDI*WSQSTAAAKSSGRKLSTC LGKIS*EFGWVKGTLALLHLIFLAPFECFNWFHV LVGSAFSILILCPPRKSVIVFLGFPSKGPSAVE VPHPVLFFCCCCV*IIEEIFVPHRIFFFF*EKP IDEKLLMKLCVRVCACNIKIQ*HLRSLNLGSCKI SN*CGINKKKKNTN

Frame 1

HARSRSRPLAALG*VSQSVGVCPRSRRSKGT*A SQLPDYSISPPSLPPRISFHPSPTLARVAMAEP SAATQSHSISSSSFGAEPSAPGGGGSPGACPALG TKSCSSSCADSFVSSSSSQPVSLFSTSQVHDLI FWRDVKKTGFVFGTTLIMLLSLAAFSVISVVSY LILALLSVTISFRIYKSVIQAVQKSEEGHPFKAY LDVDITLSSEAFHNYMNAAMVHINRALKLIIRL FLVEDLVDSLKLAVFMWLMTYVGAVFNGITLLI LAELLIFSVPIVYEKYKTQIDHYVGIARDQTKSI VEKIQAKLPGIAKKKAE*VHGNQKCNSY*NTI* *L*RRYLYYEGKYSVSA*ACIPSFFFNLVFSPI LSL*PSVSSTKIDGLIKELS*NSEEERIKFIG*V NTLMVVEPLPVA*KGKDWR*ERK*KNTSGSFCP VFSTSLTQLSIIVLPLRSHD*LMKKLFGDRSVI PSLVFFCSPQILSSCPTM*AATPDTPFL**FNYQ LDK*LIGDSDNS*FQECHLIKKNRNGKWD*EGV SRHAAVAVTPSATIPREGKAPPFGKVVSTSLDT GSEH*FENSFPNVLSSLSPAHLKFNK*GCTFLTI K*MSVTAVHCCKLVREKNNLHVGSSVIEFL*SY LSLGGNILKR*NTESLFFLIFSRDSNLRFPFGG KFFSSPSI*EFSELERKIICEFLQVLTHSHDPSE GICTGEFKVRAMIFDGPKVRQLQKVVEGNCLRV LEKLVRNLDG*KVPLPYSILFS*PPLSVLTGFM S**EVHSPSSSSALPGSQ*LSFWASPPKDLLQWK CHIQFFSFVAAVFR*LKRSLCHTGFFFFFKKNL *MKNY**NCVYVSVRAT*KYSST*GA*ILVPVK FQIDVVLIKKKKTQT

Frame 2

MRARAPAL*LRSAESVSLSESVLGAGGVKGLER ASCRIILFPLPLSRPVSLFTLLPPSLA*PWRSR RRPLSPIPSPRRPSEPSRPRPAAAGAQEPAPPW GRRAAAPPVRIPLFLPLPLSLYLYFRPHKCTI*F SGEM*RRLGLSLAPR*SCCFPWQLSVSSVWFLT SSWLFSLSPSASGSTSPSSKLYRSQKKAIHSKP TWT*TLLCPQKLSIIT*MLPWCTSTGP*NSLFVS FW*KIWLTP*SWLSSCG**PMLVLFLTESPF*F LLNCSFSVSRLSMRSTRPRLITMLASPEIRPSQ LLKRSKQNSLESPKKRQNKYMETRNATVTKTPFN SYNVVTCTMKENTQCQLEPAFQAFFLIWCFLPS FPFNPQYQAQKLMD**KNYLRTQKKKESNS*DK SIP*WW*SLYL*LERGKIGGKRENERTPLGPSVQ FSALVLLSYPL*FCP*EVMINL*KNYLGTGV*Y LPWFFFAALKSYLPAPQCEQLPLILLFFNDLTI NLINNL*VIVIIPDSKNAI**KRIEMESGTERES AGMLRWRSLPLPLSPGKERLRHLGKWFLRHWTP VLSISLRTRSRMCFPPSPLPTSSLINKVVLFLL *NKCL*LLCTAVNLLEKKITCMWAPQLLSFCDPI SVWGGTFSRGEIQKAFFS*SFPEIQISDSHLGA SFFLHLQYENSANLKEKSSVSSFRFSLIVMILQ REYALASLK*GL*YLMVPKYGSCKK*WKEIVYVS WKN*LGIWMGKRYPCLTPSYFLSPL*VF*LVSC PSRKCILHPHPLPSQEVSDCLFGLPLQRTFCSG SATSSSFLLLLLCLDN*RDLCATQDFFFFLRKTY R*KITNETVCTCLCVQHKNTVAPKELESWFL*N FKLMWY**KKKKHKQ

Sequence NM_201428

GCATGCGCGCTCGCGCTCCCGCCCTCTAGCTGCGCTCGGCTGAGTCAGTCAGTCTGTCGGAGTCTGTCCTCGGAGCAGGCGGAGTAAAGGGACTTGAGCG AGCCAGTTGCCGGATTATTCTATTTCCCCTCCCTCTCTCCCGCCCCGTATCTCTTTTCACCCTTCTCCCACCCTCGCTCGCGTAGCCATGGCGGAGCCGT CGGCGGCCACTCAGTCCCATTCCATCTCCTCGTCGTCCTTCGGAGCCGAGCCGTCCGCGCCCGGCGGCGGCGGGAGCCCAGGAGCCTGCCCCGCCCTGGG GACGAAGAGCTGCAGCTCCTCCTGTGCGGAGGGATTGAGCTCTCTTTGCTCTGATGAGCCATCTTCAGAAATTATGACTTCTTCCTTTCTTTCATCTTCT GAAATACATAACACTGGCCTTACAATACTACATGGAGAAAAAAGCCATGTGTTAGGGAGCCAGCCTATTTTAGCCAAAGAAGGAAAAGACCACTTGGATC TTCTAGATATGAAAAAGATGGAAAAGCCTCAGGGGACCAGCAACAACGTATCAGACTCTTCAGTTTCTCTTGCAGCAGGAGTTCATTGTGACCGTCCTTC TATTCCAGCCAGTTTCCCAGAGCATCCTGCTTTTCTCTCAAAGAAAATTGGTCAAGTGGAAGAGCAAATAGATAAAGAGACCAAGAACCCAAATGGGGTA TCAAGTAGGGAGGCTAAAACTGCATTGGATGCTGATGACAGATTCACTTTGCTGACAGCCCAGAAACCACCTACTGAGTACTCTAAGGTAGAAGGCATTT ATACATATTCTTTGTCTCCATCCAAAGTTTCAGGAGATGATGTTATTGAAAAGGATTCCCCTGAATCACCATTTGAAGTAATTATTGACAAAGCAGCATT TGACAAAGAATTTAAAGACTCATATAAGGAGAGCACAGATGATTTTGGTAGCTGGTCTGTGCACACTGATAAAGAATCATCCGAAGACATTTCAGAGACT AATGACAAGCTTTTTCCACTGAGAAATAAAGAGGCAGGACGTTACCCAATGTCTGCATTGCTCAGTAGGCAGTTTTCACACACAAATGCAGCACTGGAAG AGGTGTCCAGATGCGTGAATGATATGCATAACTTTACTAACGAAATACTGACTTGGGATCTGGTTCCCCAAGTGAAACAACAGACCGATAAATCTTCTGA CTGCATCACAAAAACTACAGGACTTGACATGAGTGAATATAATTCAGAAATTCCAGTTGTAAATCTTAAAACTAGCACTCATCAGAAAACTCCTGTATGT TCTATTGATGGGAGCACTCCCATCACTAAATCAACAGGTGATTGGGCAGAAGCATCTCTCCAGCAAGAAAATGCTATTACTGGAAAACCTGTACCTGACT CTTTGAATTCCACAAAAGAATTCAGTATCAAAGGTGTGCAAGGCAATATGCAGAAACAGGATGACACACTTGCAGAATTACCTGGATCTCCACCTGAGAA ATGTGACTCTTTGGGTTCTGGAGTGGCCACAGTGAAAGTGGTTTTACCTGATGACCACCTGAAAGATGAAATGGACTGGCAGAGCTCTGCATTGGGAGAA ATCACAGAAGCTGATAGTTCTGGTGAGTCTGATGACACAGTAATAGAGGACATCACAGCAGATACATCATTTGAAAATAACAAAATTCAGGCTGAAAAAC CTGTTTCCATTCCAAGTGCTGTTGTAAAAACAGGTGAAAGAGAAATCAAAGAGATTCCCAGTTGTGAGAGAGAAGAAAAAACATCTAAAAACTTTGAAGA ATTGGTCAGTGACTCTGAGCTGCATCAAGATCAGCCTGATATTCTTGGAAGGAGTCCAGCTAGTGAGGCAGCATGTTCAAAAGTACCCGATACGAATGTC TCCTTAGAAGATGTGAGTGAAGTTGCTCCTGAAAAGCCTATTACTACTGAGAACCCCAAACTTCCTTCAACAGTGTCTCCAAATGTTTTTAATGAGACAG AATTCTCATTAAATGTGACAACATCTGCCTATTTGGAGTCATTACATGGGAAAAATGTTAAACATATAGATGATTCCTCCCCAGAGGACCTGATAGCAGC CTTTACAGAAACCAGAGATAAAGGAATAGTAGATAGTGAAAGAAATGCTTTTAAAGCAATATCAGAGAAGATGACAGACTTTAAAACAACTCCTCCTGTA GAAGTCTTACATGAAAATGAGTCCGGTGGTTCTGAAATTAAAGACATTGGAAGCAAATACAGTGAACAAAGCAAAGAAACAAATGGAAGTGAGCCTCTAG GTGTTTTCCCTACCCAAGGTACTCCAGTAGCATCTCTTGACTTAGAACAAGAACAGCTCACAATTAAGGCTCTTAAAGAATTAGGTGAAAGACAGGTTGA GAAGTCAACTTCTGCACAGCGTGACGCAGAATTGCCTTCTGAAGAAGTACTGAAGCAAACTTTCACATTTGCTCCAGAATCTTGGCCACAGAGATCATAT GACATCCTAGAACGTAATGTCAAGAATGGATCTGATCTTGGGATTTCCCAGAAGCCCATCACTATCAGAGAAACTACTAGGGTAGATGCTGTTTCCAGCC TTAGCAAGACTGAATTGGTAAAAAAGCATGTCCTAGCAAGACTTCTGACAGACTTCTCAGTGCACGATCTGATTTTCTGGAGAGATGTGAAGAAGACTGG GTTTGTCTTTGGCACCACGCTGATCATGCTGCTTTCCCTGGCAGCTTTCAGTGTCATCAGTGTGGTTTCTTACCTCATCCTGGCTCTTCTCTCTGTCACC ATCAGCTTCAGGATCTACAAGTCCGTCATCCAAGCTGTACAGAAGTCAGAAGAAGGCCATCCATTCAAAGCCTACCTGGACGTAGACATTACTCTGTCCT CAGAAGCTTTCCATAATTACATGAATGCTGCCATGGTGCACATCAACAGGGCCCTGAAACTCATTATTCGTCTCTTTCTGGTAGAAGATCTGGTTGACTC CTTGAAGCTGGCTGTCTTCATGTGGCTGATGACCTATGTTGGTGCTGTTTTTAACGGAATCACCCTTCTAATTCTTGCTGAACTGCTCATTTTCAGTGTC CCGATTGTCTATGAGAAGTACAAGACCCAGATTGATCACTATGTTGGCATCGCCCGAGATCAGACCAAGTCAATTGTTGAAAAGATCCAAGCAAAACTCC CTGGAATCGCCAAAAAAAAGGCAGAATAAGTACATGGAAACCAGAAATGCAACAGTTACTAAAACACCATTTAATAGTTATAACGTCGTTACTTGTACTA TGAAGGAAAATACTCAGTGTCAGCTTGAGCCTGCATTCCAAGCTTTTTTTTTAATTTGGTGTTTTCTCCCATCCTTTCCCTTTAACCCTCAGTATCAAGC ACAAAAATTGATGGACTGATAAAAGAACTATCTTAGAACTCAGAAGAAGAAAGAATCAAATTCATAGGATAAGTCAATACCTTAATGGTGGTAGAGCCTT TACCTGTAGCTTGAAAGGGGAAAGATTGGAGGTAAGAGAGAAAATGAAAGAACACCTCTGGGTCCTTCTGTCCAGTTTTCAGCACTAGTCTTACTCAGCT ATCCATTATAGTTTTGCCCTTAAGAAGTCATGATTAACTTATGAAAAAATTATTTGGGGACAGGAGTGTGATACCTTCCTTGGTTTTTTTTTGCAGCCCT CAAATCCTATCTTCCTGCCCCACAATGTGAGCAGCTACCCCTGATACTCCTTTTCTTTAATGATTTAACTATCAACTTGATAAATAACTTATAGGTGATA GTGATAATTCCTGATTCCAAGAATGCCATCTGATAAAAAAGAATAGAAATGGAAAGTGGGACTGAGAGGGAGTCAGCAGGCATGCTGCGGTGGCGGTCAC TCCCTCTGCCACTATCCCCAGGGAAGGAAAGGCTCCGCCATTTGGGAAAGTGGTTTCTACGTCACTGGACACCGGTTCTGAGCATTAGTTTGAGAACTCG TTCCCGAATGTGCTTTCCTCCCTCTCCCCTGCCCACCTCAAGTTTAATAAATAAGGTTGTACTTTTCTTACTATAAAATAAATGTCTGTAACTGCTGTGC ACTGCTGTAAACTTGTTAGAGAAAAAAATAACCTGCATGTGGGCTCCTCAGTTATTGAGTTTTTGTGATCCTATCTCAGTCTGGGGGGGAACATTCTCAA GAGGTGAAATACAGAAAGCCTTTTTTTCTTGATCTTTTCCCGAGATTCAAATCTCCGATTCCCATTTGGGGGCAAGTTTTTTTCTTCACCTTCAATATGA GAATTCAGCGAACTTGAAAGAAAAATCATCTGTGAGTTCCTTCAGGTTCTCACTCATAGTCATGATCCTTCAGAGGGAATATGCACTGGCGAGTTTAAAG TAAGGGCTATGATATTTGATGGTCCCAAAGTACGGCAGCTGCAAAAAGTAGTGGAAGGAAATTGTCTACGTGTCTTGGAAAAATTAGTTAGGAATTTGGA TGGGTAAAAGGTACCCTTGCCTTACTCCATCTTATTTTCTTAGCCCCCTTTGAGTGTTTTAACTGGTTTCATGTCCTAGTAGGAAGTGCATTCTCCATCC TCATCCTCTGCCCTCCCAGGAAGTCAGTGATTGTCTTTTTGGGCTTCCCCTCCAAAGGACCTTCTGCAGTGGAAGTGCCACATCCAGTTCTTTTCTTTTG TTGCTGCTGTGTTTAGATAATTGAAGAGATCTTTGTGCCACACAGGATTTTTTTTTTTTTTTAAGAAAAACCTATAGATGAAAAATTACTAATGAAACTG TGTGTACGTGTCTGTGCGTGCAACATAAAAATACAGTAGCACCTAAGGAGCTTGAATCTTGGTTCCTGTAAAATTTCAAATTGATGTGGTATTAATAAAA AAAAAAAAAACACAAACAA

refseq_RTN3.F2 (forward primer)
refseq_RTN3.R2 (reverse primer)
Single exon skipping

Frame 0

ACALALPPSSCARLSQSVCRSLSSEQAE*RDLS EPVAGLFYFPSLSPAPYLFSPFSHPRSRSHGGAV GGHSVPFHLLVVLRSRAVRARRRREPRSLPRPG DEELQLLLCGGIELSLL**AIFRNYDFFLSFIF *NT*HWPYNTTWRKKPCVREPAYFSQRRKRPLGS SRYEKDGKASGDQQQRIRLFSFSCSRSSL*PSF YSSQFPRASCFSLKENWSSGRANR*RDQEPKWG IK*GG*NCIGC**QIHFADSPETTY*VL*GRRHL YIFFVSIQSFRR*CY*KGFP*ITI*SNY*QSSI *QRI*RLI*GEHR*FW*LVCAH**RIIRRHFRD **QAFSTEK*RGRTLPNVCIAQ*AVFTHKCSTGR GVQMRE*YA*LY*RNTDLGSGSPSETTDR*IF* LHHKNYRT*HE*I*FRNSSCKS*N*HSSENSCM FY*WEHSHH*INR*LGRSISPARKCYYWKTCT*L FEFHKRIQYQRCARQYAETG*HTCRITWIST*E M*LFGFWSGHSESGFT**PPER*NGLAELCIGR NHRS**FW*V**HSNRGHHSRYII*K*QNSG*KT CFHSKCCCKNR*KRNQRDSQL*ERRKNI*KL*R IGQ*L*AASRSA*YSWKESS**GSMFKSTRYEC LLRRCE*SCS*KAYYY*EPQTSFNSVSKCF**DR ILIKCDNICLFGVITWEKC*TYR*FLPRGPDSS LYRNQR*RNSR**KKCF*SNIREDDRL*NNSSC RSLT*K*VRWF*N*RHWKQIQ*TKQRNKWK*ASR CFPYPRYSSSIS*LRTRTAHN*GS*RIR*KTG* EVNFCTA*RRIAF*RSTEANFHICSRILATEII *HPRT*CQEWI*SWDFPEAHHYQRNY*GRCCFQP *QD*IGKKACPSKTSDRLLSARSDFLERCEEDW VCLWHHADHAAFPGSFQCHQCGFLPHPGSSLCH HQLQDLQVRHPSCTEVRRRPSIQSLPGRRHYSVL RSFP*LHECCHGAHQQGPETHYSSLSGRRSG*L LEAGCLHVADDLCWCCF*RNHPSNSC*TAHFQC PDCL*EVQDPD*SLCWHRPRSDQVNC*KDPSKTP WNRQKKGRISTWKPEMQQLLKHHLIVITSLLVL *RKILSVSLSLHSKLFF*FGVFSHPFPLTLSIK HKN*WTDKRTILELRRRKNQIHRISQYLNGGRAF TCSLKGERLEVREKMKEHLWVLLSSFQH*SYSA IHYSFALKKS*LTYEKIIWGQECDTFLGFFLQP SNPIFLPHNVSSYP*YSFSLMI*LST**ITYR** **FLIPRMPSDKKE*KWKVGLRGSQQACCGGGH SLCHYPQGRKGSAIWESGFYVTGHRF*ALV*EL VPECAFLPLPCPPQV**IRLYFSYYKINVCNCCA LL*TC*RKK*PACGLLSY*VFVILSQSGGEHSQ EVKYRKPFFLDLFPRFKSPIPIWGQVFFFTFNM RIQRT*KKNHL*VPSGSHS*S*SFRGNMHWRV*S KGYDI*WSQSTAAAKSSGRKLSTCLGKIS*EFG WVKGTLALLHLIFLAPFECFNWFHVLVGSAFSI LILCPPRKSVIVFLGFPSKGPSAVEVPHPVLFFC CCCV*IIEEIFVPHRIFFFF*EKPIDEKLLMKL CVRVCACNIKIQ*HLRSLNLGSCKISN*CGINK KKKNTN

Frame 1

HARSRSRPLAALG*VSQSVGVCPRSRRSKGT*A SQLPDYSISPPSLPPRISFHPSPTLARVAMAEP SAATQSHSISSSSFGAEPSAPGGGGSPGACPALG TKSCSSSCAEGLSSLCSDEPSSEIMTSSFLSSS EIHNTGLTILHGEKSHVLGSQPILAKEGKDHLD LLDMKKMEKPQGTSNNVSDSSVSLAAGVHCDRPS IPASFPEHPAFLSKKIGQVEEQIDKETKNPNGV SSREAKTALDADDRFTLLTAQKPPTEYSKVEGI YTYSLSPSKVSGDDVIEKDSPESPFEVIIDKAAF DKEFKDSYKESTDDFGSWSVHTDKESSEDISET NDKLFPLRNKEAGRYPMSALLSRQFSHTNAALE EVSRCVNDMHNFTNEILTWDLVPQVKQQTDKSSD CITKTTGLDMSEYNSEIPVVNLKTSTHQKTPVC SIDGSTPITKSTGDWAEASLQQENAITGKPVPD SLNSTKEFSIKGVQGNMQKQDDTLAELPGSPPEK CDSLGSGVATVKVVLPDDHLKDEMDWQSSALGE ITEADSSGESDDTVIEDITADTSFENNKIQAEK PVSIPSAVVKTGEREIKEIPSCEREEKTSKNFEE LVSDSELHQDQPDILGRSPASEAACSKVPDTNV SLEDVSEVAPEKPITTENPKLPSTVSPNVFNET EFSLNVTTSAYLESLHGKNVKHIDDSSPEDLIAA FTETRDKGIVDSERNAFKAISEKMTDFKTTPPV EVLHENESGGSEIKDIGSKYSEQSKETNGSEPL GVFPTQGTPVASLDLEQEQLTIKALKELGERQVE KSTSAQRDAELPSEEVLKQTFTFAPESWPQRSY DILERNVKNGSDLGISQKPITIRETTRVDAVSS LSKTELVKKHVLARLLTDFSVHDLIFWRDVKKTG FVFGTTLIMLLSLAAFSVISVVSYLILALLSVT ISFRIYKSVIQAVQKSEEGHPFKAYLDVDITLS SEAFHNYMNAAMVHINRALKLIIRLFLVEDLVDS LKLAVFMWLMTYVGAVFNGITLLILAELLIFSV PIVYEKYKTQIDHYVGIARDQTKSIVEKIQAKL PGIAKKKAE*VHGNQKCNSY*NTI**L*RRYLYY EGKYSVSA*ACIPSFFFNLVFSPILSL*PSVSS TKIDGLIKELS*NSEEERIKFIG*VNTLMVVEP LPVA*KGKDWR*ERK*KNTSGSFCPVFSTSLTQL SIIVLPLRSHD*LMKKLFGDRSVIPSLVFFCSP QILSSCPTM*AATPDTPFL**FNYQLDK*LIGD SDNS*FQECHLIKKNRNGKWD*EGVSRHAAVAVT PSATIPREGKAPPFGKVVSTSLDTGSEH*FENS FPNVLSSLSPAHLKFNK*GCTFLTIK*MSVTAV HCCKLVREKNNLHVGSSVIEFL*SYLSLGGNILK R*NTESLFFLIFSRDSNLRFPFGGKFFSSPSI* EFSELERKIICEFLQVLTHSHDPSEGICTGEFK VRAMIFDGPKVRQLQKVVEGNCLRVLEKLVRNLD G*KVPLPYSILFS*PPLSVLTGFMS**EVHSPS SSSALPGSQ*LSFWASPPKDLLQWKCHIQFFSF VAAVFR*LKRSLCHTGFFFFFKKNL*MKNY**NC VYVSVRAT*KYSST*GA*ILVPVKFQIDVVLIK KKKTQT

Frame 2

MRARAPAL*LRSAESVSLSESVLGAGGVKGLER ASCRIILFPLPLSRPVSLFTLLPPSLA*PWRSR RRPLSPIPSPRRPSEPSRPRPAAAGAQEPAPPW GRRAAAPPVRRD*ALFALMSHLQKL*LLPFFHLL KYITLALQYYMEKKAMC*GASLF*PKKEKTTWI F*I*KRWKSLRGPATTYQTLQFLLQQEFIVTVL LFQPVSQSILLFSQRKLVKWKSK*IKRPRTQMGY QVGRLKLHWMLMTDSLC*QPRNHLLSTLR*KAF IHILCLHPKFQEMMLLKRIPLNHHLK*LLTKQH LTKNLKTHIRRAQMILVAGLCTLIKNHPKTFQRL MTSFFH*EIKRQDVTQCLHCSVGSFHTQMQHWK RCPDA*MICITLLTKY*LGIWFPK*NNRPINLL TASQKLQDLT*VNIIQKFQL*ILKLALIRKLLYV LLMGALPSLNQQVIGQKHLSSKKMLLLENLYLT L*IPQKNSVSKVCKAICRNRMTHLQNYLDLHLR NVTLWVLEWPQ*KWFYLMTT*KMKWTGRALHWEK SQKLIVLVSLMTQ**RTSQQIHHLKITKFRLKN LFPFQVLL*KQVKEKSKRFPVVREKKKHLKTLK NWSVTLSCIKISLIFLEGVQLVRQHVQKYPIRMS P*KM*VKLLLKSLLLLRTPNFLQQCLQMFLMRQ NSH*M*QHLPIWSHYMGKMLNI*MIPPQRT**Q PLQKPEIKE**IVKEMLLKQYQRR*QTLKQLLL* KSYMKMSPVVLKLKTLEANTVNKAKKQMEVSL* VFSLPKVLQ*HLLT*NKNSSQLRLLKN*VKDRL RSQLLHSVTQNCLLKKY*SKLSHLLQNLGHRDHM TS*NVMSRMDLILGFPRSPSLSEKLLG*MLFPA LARLNW*KSMS*QDF*QTSQCTI*FSGEM*RRL GLSLAPR*SCCFPWQLSVSSVWFLTSSWLFSLSP SASGSTSPSSKLYRSQKKAIHSKPTWT*TLLCP QKLSIIT*MLPWCTSTGP*NSLFVSFW*KIWLT P*SWLSSCG**PMLVLFLTESPF*FLLNCSFSVS RLSMRSTRPRLITMLASPEIRPSQLLKRSKQNS LESPKKRQNKYMETRNATVTKTPFNSYNVVTCT MKENTQCQLEPAFQAFFLIWCFLPSFPFNPQYQA QKLMD**KNYLRTQKKKESNS*DKSIP*WW*SL YL*LERGKIGGKRENERTPLGPSVQFSALVLLS YPL*FCP*EVMINL*KNYLGTGV*YLPWFFFAAL KSYLPAPQCEQLPLILLFFNDLTINLINNL*VI VIIPDSKNAI**KRIEMESGTERESAGMLRWRS LPLPLSPGKERLRHLGKWFLRHWTPVLSISLRTR SRMCFPPSPLPTSSLINKVVLFLL*NKCL*LLC TAVNLLEKKITCMWAPQLLSFCDPISVWGGTFS RGEIQKAFFS*SFPEIQISDSHLGASFFLHLQYE NSANLKEKSSVSSFRFSLIVMILQREYALASLK *GL*YLMVPKYGSCKK*WKEIVYVSWKN*LGIW MGKRYPCLTPSYFLSPL*VF*LVSCPSRKCILHP HPLPSQEVSDCLFGLPLQRTFCSGSATSSSFLL LLLCLDN*RDLCATQDFFFFLRKTYR*KITNET VCTCLCVQHKNTVAPKELESWFL*NFKLMWY**K KKKHKQ