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Sequences

Sequence NM_025072

GGAGGGCAGAGAGAACCGCAACACCTGGTGCCGGGTCGGGTCGTTTCCGGGGCTTTCAGTGGCCGGAAGTCGCGGCGCCTGTACTGACTCTAGGAAGGGC TGGAGTTGTTTTGAATGGGCGCCCGTAAGAGAGGTGGGCAAGTACGTGTTACAGACGGCCACGCCGCCCTTTAGGCGGTCAAGGTGGGGCGAGGAGACGT TCGCCCCCCTGCAGTCGGCCGGGTCACTACCCAAGAGCCTTTGGAGGCGGAAGCATGGAACGGTCTGCAAACGTTCCCGAGCGGGCCTCTGCGGCTCTGG CGGGCGTTTCGAACTTGGGCGCCGGGCACACGCCCAGTCCCGAGAGCGCTGAGGGTTCCCTTAGCGTCGCCCTCACCCCGGCCAACCCGCGGGGCGCCAG AGTCCTGGCCCTTTAAACGCCGCGCGTGCCTCGGCGTCTTCGTTTCGCGCGCCCGCCCGCGGCGCCGGCGGAGCGAACATGGACCCGGCTGCGCGGGTGG TGCGGGCGCTGTGGCCTGGTGGGTGCGCCTTGGCCTGGAGGCTGGGAGGCCGCCCCCAGCCGCTGCTACCCACGCAGAGCCGGGCTGGCTTCGCGGGGGC GGCGGGCGGCCCGAGCCCCGTGGCTGCAGCTCGTAAGGGGAGCCCGCGGCTGCTGGGAGCTGCGGCGCTGGCCCTGGGGGGAGCCCTGGGGCTGTACCAC ACGGCGCGGTGGCACCTGCGCGCCCAGGACCTCCACGCAGAGCGCTCAGCCGCGCAGCTCTCCCTGTCCAGCCGCCTGCAGCTGACCCTGTACCAGTACA AGACGTGTCCCTTCTGCAGCAAGGTCCGAGCCTTCCTCGACTTCCATGCCCTGCCCTACCAGGTGGTGGAGGTGAACCCTGTGCGCAGGGCTGAGATCAA GTTCTCCTCCTACAGAAAGGTGCCCATCCTGGTGGCCCAGGAAGGAGAAAGCTCGCAACAACTAAATGACTCCTCTGTCATCATCAGCGCCCTCAAGACC TACCTGGTGTCGGGGCAGCCCCTGGAAGAGATCATCACCTACTACCCAGCCATGAAGGCTGTGAACGAGCAGGGCAAGGAGGTGACCGAGTTCGGCAATA AGTACTGGCTCATGCTCAACGAGAAGGAGGCCCAGCAAGTGTATGGTGGGAAGGAGGCCAGGACGGAGGAGATGAAGTGGCGGCAGTGGGCGGACGACTG GCTGGTGCACCTGATCTCCCCCAATGTGTACCGCACGCCCACCGAGGCTCTGGCGTCCTTTGACTACATTGTCCGCGAGGGCAAGTTCGGAGCCGTGGAG GGTGCCGTGGCCAAGTACATGGGTGCAGCGGCCATGTACCTCATCAGCAAGCGACTCAAGAGCAGGCACCGCCTCCAGGACAACGTGCGCGAGGACCTCT ATGAGGCTGCTGACAAGTGGGTGGCTGCTGTGGGCAAGGACCGGCCCTTCATGGGGGGCCAGAAGCCGAATCTCGCTGATTTGGCGGTGTATGGCGTGCT GCGTGTGATGGAGGGGCTGGATGCGTTCGATGACCTGATGCAGCACACGCACATCCAGCCCTGGTACCTGCGGGTGGAGAGGGCCATCACCGAGGCCTCC CCAGCGCACTGAATGTCCCCGCGCAGAGCAGAGGGAAGGCAGCGGAAGACGCCAGCTGCCAGGGCCTGGGGCCACTGGGCCAGCGCCTGGCGATACTGGT TGGGGGCAGGATCATTCTGCCCCTTGTCCACGCACCCCCACCAGCCCTCTCGCTTCTAACACAGGGCACCTGCTGGGGCTCAGGGATGTTAGGGACGAGT TCCAGCCCTGCCACTGCCCTGGGGCGACCCCTCCCTGTCCCTGCCTCCCTGCTCTGCCGCCCCTCTTCCTGGACCCTCAGTGGCTGTCCCATGGCTACAT CCTGTGGGTGGGGGCCCTCGACAGGACAGCAGGACGGTTTGTTTTCAGTGGAATCCCATCCCTGGGTTCCCCTGGTTCCCACTCTTCCCAAGCCTCCCGG GACTGGGACATGTTTGCAATAAAGGAAAGGTTTGTGGCGCCTGTCATGGCAGGCATCTCATGGA

refseq_PTGES2.F2 (forward primer)
refseq_PTGES2.R2 (reverse primer)
Single exon skipping

Frame 0

GGQREPQHLVPGRVVSGAFSGRKSRRLY*L*EG LELF*MGARKRGGQVRVTDGHAAL*AVKVGRGDV RPPAVGRVTTQEPLEAEAWNGLQTFPSGPLRLW RAFRTWAPGTRPVPRALRVPLASPSPRPTRGAP ESWPFKRRACLGVFVSRARPRRRRSEHGPGCAGG AGAVAWWVRLGLEAGRPPPAAATHAEPGWLRGG GGRPEPRGCSS*GEPAAAGSCGAGPGGSPGAVP HGAVAPARPGPPRRALSRAALPVQPPAADPVPVQ DVSLLQQGPSLPRLPCPALPGGGGEPCAQG*DQ VLLLQKGAHPGGPGRRKLATTK*LLCHHQRPQD LPGVGAAPGRDHHLLPSHEGCERAGQGGDRVRQ* VLAHAQREGGPASVWWEGGQDGGDEVAAVGGRL AGAPDLPQCVPHAHRGSGVL*LHCPRGQVRSRG GCRGQVHGCSGHVPHQQATQEQAPPPGQRARGPL *GC*QVGGCCGQGPALHGGPEAESR*FGGVWRA ACDGGAGCVR*PDAAHAHPALVPAGGEGHHRGL PSALNVPAQSRGKAAEDASCQGLGPLGQRLAILV GGRIILPLVHAPPPALSLLTQGTCWGSGMLGTS SSPATALGRPLPVPASLLCRPSSWTLSGCPMAT SCGWGPSTGQQDGLFSVESHPWVPLVPTLPKPPG TGTCLQ*RKGLWRLSWQASHG

Frame 1

EGRENRNTWCRVGSFPGLSVAGSRGACTDSRKG WSCFEWAPVREVGKYVLQTATPPFRRSRWGEET FAPLQSAGSLPKSLWRRKHGTVCKRSRAGLCGSG GRFELGRRAHAQSRER*GFP*RRPHPGQPAGRQ SPGPLNAARASASSFRAPARGAGGANMDPAARV VRALWPGGCALAWRLGGRPQPLLPTQSRAGFAGA AGGPSPVAAARKGSPRLLGAAALALGGALGLYH TARWHLRAQDLHAERSAAQLSLSSRLQLTLYQY KTCPFCSKVRAFLDFHALPYQVVEVNPVRRAEIK FSSYRKVPILVAQEGESSQQLNDSSVIISALKT YLVSGQPLEEIITYYPAMKAVNEQGKEVTEFGN KYWLMLNEKEAQQVYGGKEARTEEMKWRQWADDW LVHLISPNVYRTPTEALASFDYIVREGKFGAVE GAVAKYMGAAAMYLISKRLKSRHRLQDNVREDL YEAADKWVAAVGKDRPFMGGQKPNLADLAVYGVL RVMEGLDAFDDLMQHTHIQPWYLRVERAITEAS PAH*MSPRRAEGRQRKTPAARAWGHWASAWRYW LGAGSFCPLSTHPHQPSRF*HRAPAGAQGC*GRV PALPLPWGDPSLSLPPCSAAPLPGPSVAVPWLH PVGGGPRQDSRTVCFQWNPIPGFPWFPLFPSLP GLGHVCNKGKVCGACHGRHLM

Frame 2

RAERTATPGAGSGRFRGFQWPEVAAPVLTLGRA GVVLNGRP*ERWASTCYRRPRRPLGGQGGARRR SPPCSRPGHYPRAFGGGSMERSANVPERASAAL AGVSNLGAGHTPSPESAEGSLSVALTPANPRGAR VLAL*TPRVPRRLRFARPPAAPAERTWTRLRGW CGRCGLVGAPWPGGWEAAPSRCYPRRAGLASRG RRAARAPWLQLVRGARGCWELRRWPWGEPWGCTT RRGGTCAPRTSTQSAQPRSSPCPAACS*PCTST RRVPSAARSEPSSTSMPCPTRWWR*TLCAGLRS SSPPTERCPSWWPRKEKARNN*MTPLSSSAPSRP TWCRGSPWKRSSPTTQP*RL*TSRARR*PSSAI STGSCSTRRRPSKCMVGRRPGRRR*SGGSGRTT GWCT*SPPMCTARPPRLWRPLTTLSARASSEPWR VPWPSTWVQRPCTSSASDSRAGTASRTTCARTS MRLLTSGWLLWARTGPSWGARSRISLIWRCMAC CV*WRGWMRSMT*CSTRTSSPGTCGWRGPSPRPP QRTECPRAEQREGSGRRQLPGPGATGPAPGDTG WGQDHSAPCPRTPTSPLASNTGHLLGLRDVRDE FQPCHCPGATPPCPCLPALPPLFLDPQWLSHGYI LWVGALDRTAGRFVFSGIPSLGSPGSHSSQASR DWDMFAIKERFVAPVMAGISW

Sequence NM_198940

GGCTGTCCGCGGAGACGCCTATGCGGTGGAGGCTCCGGGCTTCAGCTAGGGCGGGGGAGCCCAGCAGAAGGACCGAGCAGCCTGGCACCCCACTTTGCCA TCCTCTCCCTGGAAATCTCGGGGTCGGCGGGCCGGCCGCTTCGCGTGGGCGAAATCAGAGACACGTGGTTTCCAAGGCCCCTTCGGCTCTCCCTGTCCAG CCGCCTGCAGCTGACCCTGTACCAGTACAAGACGTGTCCCTTCTGCAGCAAGGTCCGAGCCTTCCTCGACTTCCATGCCCTGCCCTACCAGGTGGTGGAG GTGAACCCTGTGCGCAGGGCTGAGATCAAGTTCTCCTCCTACAGAAAGGTGCCCATCCTGGTGGCCCAGGAAGGAGAAAGCTCGCAACAACTAAATGACT CCTCTGTCATCATCAGCGCCCTCAAGACCTACCTGGTGTCGGGGCAGCCCCTGGAAGAGATCATCACCTACTACCCAGCCATGAAGGCTGTGAACGAGCA GGGCAAGGAGGTGACCGAGTTCGGCAATAAGTACTGGCTCATGCTCAACGAGAAGGAGGCCCAGCAAGTGTATGGTGGGAAGGAGGCCAGGACGGAGGAG ATGAAGTGGCGGCAGTGGGCGGACGACTGGCTGGTGCACCTGATCTCCCCCAATGTGTACCGCACGCCCACCGAGGCTCTGGCGTCCTTTGACTACATTG TCCGCGAGGGCAAGTTCGGAGCCGTGGAGGGTGCCGTGGCCAAGTACATGGGTGCAGCGGCCATGTACCTCATCAGCAAGCGACTCAAGAGCAGGCACCG CCTCCAGGACAACGTGCGCGAGGACCTCTATGAGGCTGCTGACAAGTGGGTGGCTGCTGTGGGCAAGGACCGGCCCTTCATGGGGGGCCAGAAGCCGAAT CTCGCTGATTTGGCGGTGTATGGCGTGCTGCGTGTGATGGAGGGGCTGGATGCGTTCGATGACCTGATGCAGCACACGCACATCCAGCCCTGGTACCTGC GGGTGGAGAGGGCCATCACCGAGGCCTCCCCAGCGCACTGAATGTCCCCGCGCAGAGCAGAGGGAAGGCAGCGGAAGACGCCAGCTGCCAGGGCCTGGGG CCACTGGGCCAGCGCCTGGCGATACTGGTTGGGGGCAGGATCATTCTGCCCCTTGTCCACGCACCCCCACCAGCCCTCTCGCTTCTAACACAGGGCACCT GCTGGGGCTCAGGGATGTTAGGGACGAGTTCCAGCCCTGCCACTGCCCTGGGGCGACCCCTCCCTGTCCCTGCCTCCCTGCTCTGCCGCCCCTCTTCCTG GACCCTCAGTGGCTGTCCCATGGCTACATCCTGTGGGTGGGGGCCCTCGACAGGACAGCAGGACGGTTTGTTTTCAGTGGAATCCCATCCCTGGGTTCCC CTGGTTCCCACTCTTCCCAAGCCTCCCGGGACTGGGACATGTTTGCAATAAAGGAAAGGTTTGTGGCGCCTGTCATGGCAGGCATCTCATGGA

refseq_PTGES2.F2 (forward primer)
refseq_PTGES2.R2 (reverse primer)
Single exon skipping

Frame 0

GCPRRRLCGGGSGLQLGRGSPAEGPSSLAPHFA ILSLEISGSAGRPLRVGEIRDTWFPRPLRLSLSS RLQLTLYQYKTCPFCSKVRAFLDFHALPYQVVE VNPVRRAEIKFSSYRKVPILVAQEGESSQQLND SSVIISALKTYLVSGQPLEEIITYYPAMKAVNEQ GKEVTEFGNKYWLMLNEKEAQQVYGGKEARTEE MKWRQWADDWLVHLISPNVYRTPTEALASFDYI VREGKFGAVEGAVAKYMGAAAMYLISKRLKSRHR LQDNVREDLYEAADKWVAAVGKDRPFMGGQKPN LADLAVYGVLRVMEGLDAFDDLMQHTHIQPWYL RVERAITEASPAH*MSPRRAEGRQRKTPAARAWG HWASAWRYWLGAGSFCPLSTHPHQPSRF*HRAP AGAQGC*GRVPALPLPWGDPSLSLPPCSAAPLP GPSVAVPWLHPVGGGPRQDSRTVCFQWNPIPGFP WFPLFPSLPGLGHVCNKGKVCGACHGRHLM

Frame 1

AVRGDAYAVEAPGFS*GGGAQQKDRAAWHPTLP SSPWKSRGRRAGRFAWAKSETRGFQGPFGSPCP AACS*PCTSTRRVPSAARSEPSSTSMPCPTRWWR *TLCAGLRSSSPPTERCPSWWPRKEKARNN*MT PLSSSAPSRPTWCRGSPWKRSSPTTQP*RL*TS RARR*PSSAISTGSCSTRRRPSKCMVGRRPGRRR *SGGSGRTTGWCT*SPPMCTARPPRLWRPLTTL SARASSEPWRVPWPSTWVQRPCTSSASDSRAGT ASRTTCARTSMRLLTSGWLLWARTGPSWGARSRI SLIWRCMACCV*WRGWMRSMT*CSTRTSSPGTC GWRGPSPRPPQRTECPRAEQREGSGRRQLPGPG ATGPAPGDTGWGQDHSAPCPRTPTSPLASNTGHL LGLRDVRDEFQPCHCPGATPPCPCLPALPPLFL DPQWLSHGYILWVGALDRTAGRFVFSGIPSLGS PGSHSSQASRDWDMFAIKERFVAPVMAGISW

Frame 2

LSAETPMRWRLRASARAGEPSRRTEQPGTPLCH PLPGNLGVGGPAASRGRNQRHVVSKAPSALPVQ PPAADPVPVQDVSLLQQGPSLPRLPCPALPGGG GEPCAQG*DQVLLLQKGAHPGGPGRRKLATTK*L LCHHQRPQDLPGVGAAPGRDHHLLPSHEGCERA GQGGDRVRQ*VLAHAQREGGPASVWWEGGQDGG DEVAAVGGRLAGAPDLPQCVPHAHRGSGVL*LHC PRGQVRSRGGCRGQVHGCSGHVPHQQATQEQAP PPGQRARGPL*GC*QVGGCCGQGPALHGGPEAE SR*FGGVWRAACDGGAGCVR*PDAAHAHPALVPA GGEGHHRGLPSALNVPAQSRGKAAEDASCQGLG PLGQRLAILVGGRIILPLVHAPPPALSLLTQGT CWGSGMLGTSSSPATALGRPLPVPASLLCRPSSW TLSGCPMATSCGWGPSTGQQDGLFSVESHPWVP LVPTLPKPPGTGTCLQ*RKGLWRLSWQASHG

Sequence NM_198938

GGCTGTCCGCGGAGACGCCTATGCGGTGGAGGCTCCGGGCTTCAGCTAGGGCGGGGGAGCCCAGCAGAAGGACCGAGCAGCCTGGCACCCCACTTTGCCA TCCTCTCCCTGGAAATCTCGGGGTCGGCGGGCCGGCCGCTTCGCGTGGGCGAAATCAGAGACACGTGGTTTCCAAGGCCCCTTCGGCTCTCCCTGTCCAG CCGCCTGCAGCTGACCCTGTACCAGTACAAGACGTGTCCCTTCTGCAGCAAGGTCCGAGCCTTCCTCGACTTCCATGCCCTGCCCTACCAGGTGGTGGAG GTGAACCCTGTGCGCAGGGCTGAGATCAAGTTCTCCTCCTACAGAAAGGTGCCCATCCTGGTGGCCCAGGAAGGAGAAAGCTCGGGACGGCAGATATGAA ATGCCTGGCACAGTGCTTGGGGTCTAGTATGTAGCTTCCAGAACCCACCCTGCCCCTCTACCTGGGAGGCCAAATGGCAACAACTAAATGACTCCTCTGT CATCATCAGCGCCCTCAAGACCTACCTGGTGTCGGGGCAGCCCCTGGAAGAGATCATCACCTACTACCCAGCCATGAAGGCTGTGAACGAGCAGGGCAAG GAGGTGACCGAGTTCGGCAATAAGTACTGGCTCATGCTCAACGAGAAGGAGGCCCAGCAAGTGTATGGTGGGAAGGAGGCCAGGACGGAGGAGATGAAGT GGCGGCAGTGGGCGGACGACTGGCTGGTGCACCTGATCTCCCCCAATGTGTACCGCACGCCCACCGAGGCTCTGGCGTCCTTTGACTACATTGTCCGCGA GGGCAAGTTCGGAGCCGTGGAGGGTGCCGTGGCCAAGTACATGGGTGCAGCGGCCATGTACCTCATCAGCAAGCGACTCAAGAGCAGGCACCGCCTCCAG GACAACGTGCGCGAGGACCTCTATGAGGCTGCTGACAAGTGGGTGGCTGCTGTGGGCAAGGACCGGCCCTTCATGGGGGGCCAGAAGCCGAATCTCGCTG ATTTGGCGGTGTATGGCGTGCTGCGTGTGATGGAGGGGCTGGATGCGTTCGATGACCTGATGCAGCACACGCACATCCAGCCCTGGTACCTGCGGGTGGA GAGGGCCATCACCGAGGCCTCCCCAGCGCACTGAATGTCCCCGCGCAGAGCAGAGGGAAGGCAGCGGAAGACGCCAGCTGCCAGGGCCTGGGGCCACTGG GCCAGCGCCTGGCGATACTGGTTGGGGGCAGGATCATTCTGCCCCTTGTCCACGCACCCCCACCAGCCCTCTCGCTTCTAACACAGGGCACCTGCTGGGG CTCAGGGATGTTAGGGACGAGTTCCAGCCCTGCCACTGCCCTGGGGCGACCCCTCCCTGTCCCTGCCTCCCTGCTCTGCCGCCCCTCTTCCTGGACCCTC AGTGGCTGTCCCATGGCTACATCCTGTGGGTGGGGGCCCTCGACAGGACAGCAGGACGGTTTGTTTTCAGTGGAATCCCATCCCTGGGTTCCCCTGGTTC CCACTCTTCCCAAGCCTCCCGGGACTGGGACATGTTTGCAATAAAGGAAAGGTTTGTGGCGCCTGTCATGGCAGGCATCTCATGGA

refseq_PTGES2.F2 (forward primer)
refseq_PTGES2.R2 (reverse primer)
Single exon skipping

Frame 0

GCPRRRLCGGGSGLQLGRGSPAEGPSSLAPHFA ILSLEISGSAGRPLRVGEIRDTWFPRPLRLSLSS RLQLTLYQYKTCPFCSKVRAFLDFHALPYQVVE VNPVRRAEIKFSSYRKVPILVAQEGESSGRQI* NAWHSAWGLVCSFQNPPCPSTWEAKWQQLNDSSV IISALKTYLVSGQPLEEIITYYPAMKAVNEQGK EVTEFGNKYWLMLNEKEAQQVYGGKEARTEEMK WRQWADDWLVHLISPNVYRTPTEALASFDYIVRE GKFGAVEGAVAKYMGAAAMYLISKRLKSRHRLQ DNVREDLYEAADKWVAAVGKDRPFMGGQKPNLA DLAVYGVLRVMEGLDAFDDLMQHTHIQPWYLRVE RAITEASPAH*MSPRRAEGRQRKTPAARAWGHW ASAWRYWLGAGSFCPLSTHPHQPSRF*HRAPAG AQGC*GRVPALPLPWGDPSLSLPPCSAAPLPGPS VAVPWLHPVGGGPRQDSRTVCFQWNPIPGFPWF PLFPSLPGLGHVCNKGKVCGACHGRHLM

Frame 1

AVRGDAYAVEAPGFS*GGGAQQKDRAAWHPTLP SSPWKSRGRRAGRFAWAKSETRGFQGPFGSPCP AACS*PCTSTRRVPSAARSEPSSTSMPCPTRWWR *TLCAGLRSSSPPTERCPSWWPRKEKARDGRYE MPGTVLGV*YVASRTHPAPLPGRPNGNN*MTPL SSSAPSRPTWCRGSPWKRSSPTTQP*RL*TSRAR R*PSSAISTGSCSTRRRPSKCMVGRRPGRRR*S GGSGRTTGWCT*SPPMCTARPPRLWRPLTTLSA RASSEPWRVPWPSTWVQRPCTSSASDSRAGTASR TTCARTSMRLLTSGWLLWARTGPSWGARSRISL IWRCMACCV*WRGWMRSMT*CSTRTSSPGTCGW RGPSPRPPQRTECPRAEQREGSGRRQLPGPGATG PAPGDTGWGQDHSAPCPRTPTSPLASNTGHLLG LRDVRDEFQPCHCPGATPPCPCLPALPPLFLDP QWLSHGYILWVGALDRTAGRFVFSGIPSLGSPGS HSSQASRDWDMFAIKERFVAPVMAGISW

Frame 2

LSAETPMRWRLRASARAGEPSRRTEQPGTPLCH PLPGNLGVGGPAASRGRNQRHVVSKAPSALPVQ PPAADPVPVQDVSLLQQGPSLPRLPCPALPGGG GEPCAQG*DQVLLLQKGAHPGGPGRRKLGTADMK CLAQCLGSSM*LPEPTLPLYLGGQMATTK*LLC HHQRPQDLPGVGAAPGRDHHLLPSHEGCERAGQ GGDRVRQ*VLAHAQREGGPASVWWEGGQDGGDEV AAVGGRLAGAPDLPQCVPHAHRGSGVL*LHCPR GQVRSRGGCRGQVHGCSGHVPHQQATQEQAPPP GQRARGPL*GC*QVGGCCGQGPALHGGPEAESR* FGGVWRAACDGGAGCVR*PDAAHAHPALVPAGG EGHHRGLPSALNVPAQSRGKAAEDASCQGLGPL GQRLAILVGGRIILPLVHAPPPALSLLTQGTCWG SGMLGTSSSPATALGRPLPVPASLLCRPSSWTL SGCPMATSCGWGPSTGQQDGLFSVESHPWVPLV PTLPKPPGTGTCLQ*RKGLWRLSWQASHG

Sequence NM_198939

GGAGGGCAGAGAGAACCGCAACACCTGGTGCCGGGTCGGGTCGTTTCCGGGGCTTTCAGTGGCCGGAAGTCGCGGCGCCTGTACTGACTCTAGGAAGGGC TGGAGTTGTTTTGAATGGGCGCCCGTAAGAGAGGTGGGCAAGTACGTGTTACAGACGGCCACGCCGCCCTTTAGGCGGTCAAGGTGGGGCGAGGAGACGT TCGCCCCCCTGCAGTCGGCCGGGTCACTACCCAAGAGCCTTTGGAGGCGGAAGCATGGAACGGTCTGCAAACGTTCCCGAGCGGGCCTCTGCGGCTCTGG CGGGCGTTTCGAACTTGGGCGCCGGGCACACGCCCAGTCCCGAGAGCGCTGAGGGTTCCCTTAGCGTCGCCCTCACCCCGGCCAACCCGCGGGGCGCCAG AGTCCTGGCCCTTTAAACGCCGCGCGTGCCTCGGCGTCTTCGTTTCGCGCGCCCGCCCGCGGCGCCGGCGGAGCGAACATGGACCCGGCTGCGCGGGTGG TGCGGGCGCTGTGGCCTGGTGGGTGCGCCTTGGCCTGGAGGCTGGGAGGCCGCCCCCAGCCGCTGCTACCCACGCAGAGCCGGGCTGGCTTCGCGGGGGC GGCGGGCGGCCCGAGCCCCGTGGCTGCAGCTCGTAAGGGGAGCCCGCGGCTGCTGGGAGCTGCGGCGCTGGCCCTGGGGGGAGCCCTGGGGCTGTACCAC ACGGCGCGGTGGCACCTGCGCGCCCAGGACCTCCACGCAGAGCGCTCAGCCGCGCAGCTCTCCCTGTCCAGCCGCCTGCAGCTGACCCTGTACCAGTACA AGACGTGTCCCTTCTGCAGCAAGGTCCGAGCCTTCCTCGACTTCCATGCCCTGCCCTACCAGGTGGTGGAGGTGAACCCTGTGCGCAGGGCTGAGATCAA GTTCTCCTCCTACAGAAAGGTGCCCATCCTGGTGGCCCAGGAAGGAGAAAGCTCGGGACGGCAGATATGAAATGCCTGGCACAGTGCTTGGGGTCTAGTA TGTAGCTTCCAGAACCCACCCTGCCCCTCTACCTGGGAGGCCAAATGGCAACAACTAAATGACTCCTCTGTCATCATCAGCGCCCTCAAGACCTACCTGG TGTCGGGGCAGCCCCTGGAAGAGATCATCACCTACTACCCAGCCATGAAGGCTGTGAACGAGCAGGGCAAGGAGGTGACCGAGTTCGGCAATAAGTACTG GCTCATGCTCAACGAGAAGGAGGCCCAGCAAGTGTATGGTGGGAAGGAGGCCAGGACGGAGGAGATGAAGTGGCGGCAGTGGGCGGACGACTGGCTGGTG CACCTGATCTCCCCCAATGTGTACCGCACGCCCACCGAGGCTCTGGCGTCCTTTGACTACATTGTCCGCGAGGGCAAGTTCGGAGCCGTGGAGGGTGCCG TGGCCAAGTACATGGGTGCAGCGGCCATGTACCTCATCAGCAAGCGACTCAAGAGCAGGCACCGCCTCCAGGACAACGTGCGCGAGGACCTCTATGAGGC TGCTGACAAGTGGGTGGCTGCTGTGGGCAAGGACCGGCCCTTCATGGGGGGCCAGAAGCCGAATCTCGCTGATTTGGCGGTGTATGGCGTGCTGCGTGTG ATGGAGGGGCTGGATGCGTTCGATGACCTGATGCAGCACACGCACATCCAGCCCTGGTACCTGCGGGTGGAGAGGGCCATCACCGAGGCCTCCCCAGCGC ACTGAATGTCCCCGCGCAGAGCAGAGGGAAGGCAGCGGAAGACGCCAGCTGCCAGGGCCTGGGGCCACTGGGCCAGCGCCTGGCGATACTGGTTGGGGGC AGGATCATTCTGCCCCTTGTCCACGCACCCCCACCAGCCCTCTCGCTTCTAACACAGGGCACCTGCTGGGGCTCAGGGATGTTAGGGACGAGTTCCAGCC CTGCCACTGCCCTGGGGCGACCCCTCCCTGTCCCTGCCTCCCTGCTCTGCCGCCCCTCTTCCTGGACCCTCAGTGGCTGTCCCATGGCTACATCCTGTGG GTGGGGGCCCTCGACAGGACAGCAGGACGGTTTGTTTTCAGTGGAATCCCATCCCTGGGTTCCCCTGGTTCCCACTCTTCCCAAGCCTCCCGGGACTGGG ACATGTTTGCAATAAAGGAAAGGTTTGTGGCGCCTGTCATGGCAGGCATCTCATGGA

refseq_PTGES2.F2 (forward primer)
refseq_PTGES2.R2 (reverse primer)
Single exon skipping

Frame 0

GGQREPQHLVPGRVVSGAFSGRKSRRLY*L*EG LELF*MGARKRGGQVRVTDGHAAL*AVKVGRGDV RPPAVGRVTTQEPLEAEAWNGLQTFPSGPLRLW RAFRTWAPGTRPVPRALRVPLASPSPRPTRGAP ESWPFKRRACLGVFVSRARPRRRRSEHGPGCAGG AGAVAWWVRLGLEAGRPPPAAATHAEPGWLRGG GGRPEPRGCSS*GEPAAAGSCGAGPGGSPGAVP HGAVAPARPGPPRRALSRAALPVQPPAADPVPVQ DVSLLQQGPSLPRLPCPALPGGGGEPCAQG*DQ VLLLQKGAHPGGPGRRKLGTADMKCLAQCLGSS M*LPEPTLPLYLGGQMATTK*LLCHHQRPQDLPG VGAAPGRDHHLLPSHEGCERAGQGGDRVRQ*VL AHAQREGGPASVWWEGGQDGGDEVAAVGGRLAG APDLPQCVPHAHRGSGVL*LHCPRGQVRSRGGCR GQVHGCSGHVPHQQATQEQAPPPGQRARGPL*G C*QVGGCCGQGPALHGGPEAESR*FGGVWRAAC DGGAGCVR*PDAAHAHPALVPAGGEGHHRGLPSA LNVPAQSRGKAAEDASCQGLGPLGQRLAILVGG RIILPLVHAPPPALSLLTQGTCWGSGMLGTSSS PATALGRPLPVPASLLCRPSSWTLSGCPMATSCG WGPSTGQQDGLFSVESHPWVPLVPTLPKPPGTG TCLQ*RKGLWRLSWQASHG

Frame 1

EGRENRNTWCRVGSFPGLSVAGSRGACTDSRKG WSCFEWAPVREVGKYVLQTATPPFRRSRWGEET FAPLQSAGSLPKSLWRRKHGTVCKRSRAGLCGSG GRFELGRRAHAQSRER*GFP*RRPHPGQPAGRQ SPGPLNAARASASSFRAPARGAGGANMDPAARV VRALWPGGCALAWRLGGRPQPLLPTQSRAGFAGA AGGPSPVAAARKGSPRLLGAAALALGGALGLYH TARWHLRAQDLHAERSAAQLSLSSRLQLTLYQY KTCPFCSKVRAFLDFHALPYQVVEVNPVRRAEIK FSSYRKVPILVAQEGESSGRQI*NAWHSAWGLV CSFQNPPCPSTWEAKWQQLNDSSVIISALKTYL VSGQPLEEIITYYPAMKAVNEQGKEVTEFGNKYW LMLNEKEAQQVYGGKEARTEEMKWRQWADDWLV HLISPNVYRTPTEALASFDYIVREGKFGAVEGA VAKYMGAAAMYLISKRLKSRHRLQDNVREDLYEA ADKWVAAVGKDRPFMGGQKPNLADLAVYGVLRV MEGLDAFDDLMQHTHIQPWYLRVERAITEASPA H*MSPRRAEGRQRKTPAARAWGHWASAWRYWLGA GSFCPLSTHPHQPSRF*HRAPAGAQGC*GRVPA LPLPWGDPSLSLPPCSAAPLPGPSVAVPWLHPV GGGPRQDSRTVCFQWNPIPGFPWFPLFPSLPGLG HVCNKGKVCGACHGRHLM

Frame 2

RAERTATPGAGSGRFRGFQWPEVAAPVLTLGRA GVVLNGRP*ERWASTCYRRPRRPLGGQGGARRR SPPCSRPGHYPRAFGGGSMERSANVPERASAAL AGVSNLGAGHTPSPESAEGSLSVALTPANPRGAR VLAL*TPRVPRRLRFARPPAAPAERTWTRLRGW CGRCGLVGAPWPGGWEAAPSRCYPRRAGLASRG RRAARAPWLQLVRGARGCWELRRWPWGEPWGCTT RRGGTCAPRTSTQSAQPRSSPCPAACS*PCTST RRVPSAARSEPSSTSMPCPTRWWR*TLCAGLRS SSPPTERCPSWWPRKEKARDGRYEMPGTVLGV*Y VASRTHPAPLPGRPNGNN*MTPLSSSAPSRPTW CRGSPWKRSSPTTQP*RL*TSRARR*PSSAIST GSCSTRRRPSKCMVGRRPGRRR*SGGSGRTTGWC T*SPPMCTARPPRLWRPLTTLSARASSEPWRVP WPSTWVQRPCTSSASDSRAGTASRTTCARTSMR LLTSGWLLWARTGPSWGARSRISLIWRCMACCV* WRGWMRSMT*CSTRTSSPGTCGWRGPSPRPPQR TECPRAEQREGSGRRQLPGPGATGPAPGDTGWG QDHSAPCPRTPTSPLASNTGHLLGLRDVRDEFQP CHCPGATPPCPCLPALPPLFLDPQWLSHGYILW VGALDRTAGRFVFSGIPSLGSPGSHSSQASRDW DMFAIKERFVAPVMAGISW