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Sequence NM_147158
CTCCGAGGCCGTGAGCGCAAAGCCTCAGGCCCCGGCTCCCTCCTGAGCTGCGCCGTGCCAGGCCGCCCGCCGGGATGCAGTGGGCCGTGGGCCGGCGGTGGGCGTGGGCCGCGCTGCTCCTGGCTGTCGCAGCGGTGCTGACCCAGGTCGTCTGGCTCTGGCTGGACCACGAGCTGGCCTTCTCTCGTCTGATCGAGGAG
CTGCAGTGGGTGTTCGTGAATGCGGGTGGCTGGATGGGCGCCATGTGCCTTCTGCACGCCTCGCTGTCCGAGTATGTGCTGCTCTTCGGCACCGCCTTGG
GCTCCCGCGGCCACTCGGGGCGCTACTGGGCTGAGATCTCGGATACCATCATCTCTGGCACCTTCCACCAGTGGAGAGAGGGCACCACCAAAAGTGAGGT
CTTCTACCCAGGGGAGACGGTAGTACACGGGCCTGGTGAGGCAACAGCTGTGGAGTGGGGGCCAAACACATGGATGGTGGAGTACGGCCGGGGCGTCATC
CCATCCACCCTGGCCTTCGCGCTGGCCGACACTGTCTTCAGCACCCAGGACTTCCTCACCCTCTTCTATACTCTTCGCTCCTATGCTCGGGGCCTCCGGC
TTGAGCTCACCACCTACCTCTTTGGCCAGGACCCTTGACCAGCCAGGCCTGAAGGAAGACCTGCGGATAGACAGGAGCGGGCAGGCCCGCACATATCCAC
TTGCTGGAGCCCATGTTTACAGACAGGGACATACACCATGCAGATCCTGAGTTCCTGCTGTATGAGCAGGGATATCCATGCTTATGTATCCAAACACAGA
GACCCATGGGAACAAATGAGACACATATAGATACTGAGACCTGTGTGTACAGTAGGACCATGCACTCACACCCATCTGGAGAGGGAGCCCCCGGTATACC
AAGGGAGCCAGTTGTGTTCAGACACACACATCACAGCTTGACTCACTAACTGAGGCCTTTCCATAGCTCCACAGCTTCCCACCTCCTCCCCACCAAACCG
GGGTTCTAGAGTTAAGGATGGGGGAGGGTATTATACTGCCTCAGTCTGACTCCTCAACCCAGCAGCAATTTGAGGGGATGAGGGGGAAGAGGAGCTGCCT
TTTGGAGGCCCCCTTCACCTGCAGCTATGATGCCCTTCCCCTTCTCCCCTGTCCTCACCATATGCCTTATCCCCATTCTACTCCCCTGCTATGCAAGTGC
CCCTGTGGCTTGTCCCCAACCCCCTCAGCAACAAAGCTCAGCTGGGGAACGAGAGTAATTTGAAGAATGCTTGAAGTCAGCGTCTTCCATTCCAGAAAGA
CCCCCATTCTTCCTTTGGGGGTATGATGTGGAAGCTGGTTTCAGCCCAGGACCCACCACTGAGGAGAGGATCTAGACAGGTGGGCCTAATTCCAAGGGGC
CCTTCCTGGCCTGGAGAAGGCCTTTTACACACACACAACACATACACACACACACACACACACACATATCACAGTTTTCACACAGCCCCTGCTGCATTCT
CTGTCCATCTGTCTGTTTCTATTAATAAAGATTTGTTGATCTGTTCCA
- refseq_OPRS1.F2 (forward primer)
- refseq_OPRS1.R2 (reverse primer)
- Intron retention
Frame 0
LRGRERKASGPGSLLSCAVPGRPPGCSGPWAGGGRGPRCSWLSQRC*PRSSGSGWTTSWPSLV*SRS
CSGCS*MRVAGWAPCAFCTPRCPSMCCSSAPPW
APAATRGATGLRSRIPSSLAPSTSGERAPPKVR
SSTQGRR*YTGLVRQQLWSGGQTHGWWSTAGASS
HPPWPSRWPTLSSAPRTSSPSSILFAPMLGASG
LSSPPTSLARTLDQPGLKEDLRIDRSGQARTYP
LAGAHVYRQGHTPCRS*VPAV*AGISMLMYPNTE
THGNK*DTYRY*DLCVQ*DHALTPIWRGSPRYT
KGASCVQTHTSQLDSLTEAFP*LHSFPPPPHQT
GVLELRMGEGIILPQSDSSTQQQFEGMRGKRSCL
LEAPFTCSYDALPLLPCPHHMPYPHSTPLLCKC
PCGLSPTPSATKLSWGTRVI*RMLEVSVFHSRK
TPILPLGV*CGSWFQPRTHH*GEDLDRWA*FQGA
LPGLEKAFYTHTTHTHTHTHTHITVFTQPLLHS
LSICLFLLIKIC*SVP
Frame 1
SEAVSAKPQAPAPS*AAPCQAARRDAVGRGPAVGVGRAAPGCRSGADPGRLALAGPRAGLLSSDRG
AAVGVRECGWLDGRHVPSARLAVRVCAALRHRLG
LPRPLGALLG*DLGYHHLWHLPPVERGHHQK*G
LLPRGDGSTRAW*GNSCGVGAKHMDGGVRPGRH
PIHPGLRAGRHCLQHPGLPHPLLYSSLLCSGPPA
*AHHLPLWPGPLTSQA*RKTCG*TGAGRPAHIH
LLEPMFTDRDIHHADPEFLLYEQGYPCLCIQTQ
RPMGTNETHIDTETCVYSRTMHSHPSGEGAPGIP
REPVVFRHTHHSLTH*LRPFHSSTASHLLPTKP
GF*S*GWGRVLYCLSLTPQPSSNLRG*GGRGAA
FWRPPSPAAMMPFPFSPVLTICLIPILLPCYASA
PVACPQPPQQQSSAGERE*FEECLKSASSIPER
PPFFLWGYDVEAGFSPGPTTEERI*TGGPNSKG
PFLAWRRPFTHTQHIHTHTHTHISQFSHSPCCIL
CPSVCFY**RFVDLF
Frame 2
PRP*AQSLRPRLPPELRRARPPAGMQWAVGRRWAWAALLLAVAAVLTQVVWLWLDHELAFSRLIEE
LQWVFVNAGGWMGAMCLLHASLSEYVLLFGTAL
GSRGHSGRYWAEISDTIISGTFHQWREGTTKSEV
FYPGETVVHGPGEATAVEWGPNTWMVEYGRGVI
PSTLAFALADTVFSTQDFLTLFYTLRSYARGLR
LELTTYLFGQDP*PARPEGRPADRQERAGPHIST
CWSPCLQTGTYTMQILSSCCMSRDIHAYVSKHR
DPWEQMRHI*ILRPVCTVGPCTHTHLEREPPVY
QGSQLCSDTHITA*LTN*GLSIAPQLPTSSPPNR
GSRVKDGGGYYTASV*LLNPAAI*GDEGEEELP
FGGPLHLQL*CPSPSPLSSPYALSPFYSPAMQV
PLWLVPNPLSNKAQLGNESNLKNA*SQRLPFQKD
PHSSFGGMMWKLVSAQDPPLRRGSRQVGLIPRG
PSWPGEGLLHTHNTYTHTHTHTYHSFHTAPAAF
SVHLSVSINKDLLICS
Sequence NM_005866
CTCCGAGGCCGTGAGCGCAAAGCCTCAGGCCCCGGCTCCCTCCTGAGCTGCGCCGTGCCAGGCCGCCCGCCGGGATGCAGTGGGCCGTGGGCCGGCGGTGGGCGTGGGCCGCGCTGCTCCTGGCTGTCGCAGCGGTGCTGACCCAGGTCGTCTGGCTCTGGCTGGGTACGCAGAGCTTCGTCTTCCAGCGCGAAGAGATA
GCGCAGTTGGCGCGGCAGTACGCTGGGCTGGACCACGAGCTGGCCTTCTCTCGTCTGATCGTGGAGCTGCGGCGGCTGCACCCAGGCCACGTGCTGCCCG
ACGAGGAGCTGCAGTGGGTGTTCGTGAATGCGGGTGGCTGGATGGGCGCCATGTGCCTTCTGCACGCCTCGCTGTCCGAGTATGTGCTGCTCTTCGGCAC
CGCCTTGGGCTCCCGCGGCCACTCGGGGCGCTACTGGGCTGAGATCTCGGATACCATCATCTCTGGCACCTTCCACCAGTGGAGAGAGGGCACCACCAAA
AGTGAGGTCTTCTACCCAGGGGAGACGGTAGTACACGGGCCTGGTGAGGCAACAGCTGTGGAGTGGGGGCCAAACACATGGATGGTGGAGTACGGCCGGG
GCGTCATCCCATCCACCCTGGCCTTCGCGCTGGCCGACACTGTCTTCAGCACCCAGGACTTCCTCACCCTCTTCTATACTCTTCGCTCCTATGCTCGGGG
CCTCCGGCTTGAGCTCACCACCTACCTCTTTGGCCAGGACCCTTGACCAGCCAGGCCTGAAGGAAGACCTGCGGATAGACAGGAGCGGGCAGGCCCGCAC
ATATCCACTTGCTGGAGCCCATGTTTACAGACAGGGACATACACCATGCAGATCCTGAGTTCCTGCTGTATGAGCAGGGATATCCATGCTTATGTATCCA
AACACAGAGACCCATGGGAACAAATGAGACACATATAGATACTGAGACCTGTGTGTACAGTAGGACCATGCACTCACACCCATCTGGAGAGGGAGCCCCC
GGTATACCAAGGGAGCCAGTTGTGTTCAGACACACACATCACAGCTTGACTCACTAACTGAGGCCTTTCCATAGCTCCACAGCTTCCCACCTCCTCCCCA
CCAAACCGGGGTTCTAGAGTTAAGGATGGGGGAGGGTATTATACTGCCTCAGTCTGACTCCTCAACCCAGCAGCAATTTGAGGGGATGAGGGGGAAGAGG
AGCTGCCTTTTGGAGGCCCCCTTCACCTGCAGCTATGATGCCCTTCCCCTTCTCCCCTGTCCTCACCATATGCCTTATCCCCATTCTACTCCCCTGCTAT
GCAAGTGCCCCTGTGGCTTGTCCCCAACCCCCTCAGCAACAAAGCTCAGCTGGGGAACGAGAGTAATTTGAAGAATGCTTGAAGTCAGCGTCTTCCATTC
CAGAAAGACCCCCATTCTTCCTTTGGGGGTATGATGTGGAAGCTGGTTTCAGCCCAGGACCCACCACTGAGGAGAGGATCTAGACAGGTGGGCCTAATTC
CAAGGGGCCCTTCCTGGCCTGGAGAAGGCCTTTTACACACACACAACACATACACACACACACACACACACACATATCACAGTTTTCACACAGCCCCTGC
TGCATTCTCTGTCCATCTGTCTGTTTCTATTAATAAAGATTTGTTGATCTGTTCCA
- refseq_OPRS1.F2 (forward primer)
- refseq_OPRS1.R2 (reverse primer)
- Intron retention
Frame 0
LRGRERKASGPGSLLSCAVPGRPPGCSGPWAGGGRGPRCSWLSQRC*PRSSGSGWVRRASSSSAKR*
RSWRGSTLGWTTSWPSLV*SWSCGGCTQATCCP
TRSCSGCS*MRVAGWAPCAFCTPRCPSMCCSSA
PPWAPAATRGATGLRSRIPSSLAPSTSGERAPPK
VRSSTQGRR*YTGLVRQQLWSGGQTHGWWSTAG
ASSHPPWPSRWPTLSSAPRTSSPSSILFAPMLG
ASGLSSPPTSLARTLDQPGLKEDLRIDRSGQART
YPLAGAHVYRQGHTPCRS*VPAV*AGISMLMYP
NTETHGNK*DTYRY*DLCVQ*DHALTPIWRGSP
RYTKGASCVQTHTSQLDSLTEAFP*LHSFPPPPH
QTGVLELRMGEGIILPQSDSSTQQQFEGMRGKR
SCLLEAPFTCSYDALPLLPCPHHMPYPHSTPLL
CKCPCGLSPTPSATKLSWGTRVI*RMLEVSVFHS
RKTPILPLGV*CGSWFQPRTHH*GEDLDRWA*F
QGALPGLEKAFYTHTTHTHTHTHTHITVFTQPL
LHSLSICLFLLIKIC*SVP
Frame 1
SEAVSAKPQAPAPS*AAPCQAARRDAVGRGPAVGVGRAAPGCRSGADPGRLALAGYAELRLPARRD
SAVGAAVRWAGPRAGLLSSDRGAAAAAPRPRAAR
RGAAVGVRECGWLDGRHVPSARLAVRVCAALRH
RLGLPRPLGALLG*DLGYHHLWHLPPVERGHHQ
K*GLLPRGDGSTRAW*GNSCGVGAKHMDGGVRPG
RHPIHPGLRAGRHCLQHPGLPHPLLYSSLLCSG
PPA*AHHLPLWPGPLTSQA*RKTCG*TGAGRPA
HIHLLEPMFTDRDIHHADPEFLLYEQGYPCLCIQ
TQRPMGTNETHIDTETCVYSRTMHSHPSGEGAP
GIPREPVVFRHTHHSLTH*LRPFHSSTASHLLP
TKPGF*S*GWGRVLYCLSLTPQPSSNLRG*GGRG
AAFWRPPSPAAMMPFPFSPVLTICLIPILLPCY
ASAPVACPQPPQQQSSAGERE*FEECLKSASSI
PERPPFFLWGYDVEAGFSPGPTTEERI*TGGPNS
KGPFLAWRRPFTHTQHIHTHTHTHISQFSHSPC
CILCPSVCFY**RFVDLF
Frame 2
PRP*AQSLRPRLPPELRRARPPAGMQWAVGRRWAWAALLLAVAAVLTQVVWLWLGTQSFVFQREEI
AQLARQYAGLDHELAFSRLIVELRRLHPGHVLP
DEELQWVFVNAGGWMGAMCLLHASLSEYVLLFGT
ALGSRGHSGRYWAEISDTIISGTFHQWREGTTK
SEVFYPGETVVHGPGEATAVEWGPNTWMVEYGR
GVIPSTLAFALADTVFSTQDFLTLFYTLRSYARG
LRLELTTYLFGQDP*PARPEGRPADRQERAGPH
ISTCWSPCLQTGTYTMQILSSCCMSRDIHAYVS
KHRDPWEQMRHI*ILRPVCTVGPCTHTHLEREPP
VYQGSQLCSDTHITA*LTN*GLSIAPQLPTSSP
PNRGSRVKDGGGYYTASV*LLNPAAI*GDEGEE
ELPFGGPLHLQL*CPSPSPLSSPYALSPFYSPAM
QVPLWLVPNPLSNKAQLGNESNLKNA*SQRLPF
QKDPHSSFGGMMWKLVSAQDPPLRRGSRQVGLI
PRGPSWPGEGLLHTHNTYTHTHTHTYHSFHTAPA
AFSVHLSVSINKDLLICS
Sequence NM_147157
CTCCGAGGCCGTGAGCGCAAAGCCTCAGGCCCCGGCTCCCTCCTGAGCTGCGCCGTGCCAGGCCGCCCGCCGGGATGCAGTGGGCCGTGGGCCGGCGGTGGGCGTGGGCCGCGCTGCTCCTGGCTGTCGCAGCGGTGCTGACCCAGGTCGTCTGGCTCTGGCTGGGTACGCAGAGCTTCGTCTTCCAGCGCGAAGAGATA
GCGCAGTTGGCGCGGCAGTACGCTGGGCTGGACCACGAGCTGGCCTTCTCTCGTCTGATCGTGGAGCTGCGGCGGCTGCACCCAGGCCACGTGCTGCCCG
ACGAGGAGCTGCAGTGGGTGTTCGTGAATGCGGGTGGCTGGATGGGCGCCATGTGCCTTCTGCACGCCTCGCTGTCCGAGTATGTGCTGCTCTTCGGCAC
CGCCTTGGGCTCCCGCGGCCACTCGGGGGAGACGGTAGTACACGGGCCTGGTGAGGCAACAGCTGTGGAGTGGGGGCCAAACACATGGATGGTGGAGTAC
GGCCGGGGCGTCATCCCATCCACCCTGGCCTTCGCGCTGGCCGACACTGTCTTCAGCACCCAGGACTTCCTCACCCTCTTCTATACTCTTCGCTCCTATG
CTCGGGGCCTCCGGCTTGAGCTCACCACCTACCTCTTTGGCCAGGACCCTTGACCAGCCAGGCCTGAAGGAAGACCTGCGGATAGACAGGAGCGGGCAGG
CCCGCACATATCCACTTGCTGGAGCCCATGTTTACAGACAGGGACATACACCATGCAGATCCTGAGTTCCTGCTGTATGAGCAGGGATATCCATGCTTAT
GTATCCAAACACAGAGACCCATGGGAACAAATGAGACACATATAGATACTGAGACCTGTGTGTACAGTAGGACCATGCACTCACACCCATCTGGAGAGGG
AGCCCCCGGTATACCAAGGGAGCCAGTTGTGTTCAGACACACACATCACAGCTTGACTCACTAACTGAGGCCTTTCCATAGCTCCACAGCTTCCCACCTC
CTCCCCACCAAACCGGGGTTCTAGAGTTAAGGATGGGGGAGGGTATTATACTGCCTCAGTCTGACTCCTCAACCCAGCAGCAATTTGAGGGGATGAGGGG
GAAGAGGAGCTGCCTTTTGGAGGCCCCCTTCACCTGCAGCTATGATGCCCTTCCCCTTCTCCCCTGTCCTCACCATATGCCTTATCCCCATTCTACTCCC
CTGCTATGCAAGTGCCCCTGTGGCTTGTCCCCAACCCCCTCAGCAACAAAGCTCAGCTGGGGAACGAGAGTAATTTGAAGAATGCTTGAAGTCAGCGTCT
TCCATTCCAGAAAGACCCCCATTCTTCCTTTGGGGGTATGATGTGGAAGCTGGTTTCAGCCCAGGACCCACCACTGAGGAGAGGATCTAGACAGGTGGGC
CTAATTCCAAGGGGCCCTTCCTGGCCTGGAGAAGGCCTTTTACACACACACAACACATACACACACACACACACACACACATATCACAGTTTTCACACAG
CCCCTGCTGCATTCTCTGTCCATCTGTCTGTTTCTATTAATAAAGATTTGTTGATCTGTTCCA
- refseq_OPRS1.F2 (forward primer)
- refseq_OPRS1.R2 (reverse primer)
- Intron retention
Frame 0
LRGRERKASGPGSLLSCAVPGRPPGCSGPWAGGGRGPRCSWLSQRC*PRSSGSGWVRRASSSSAKR*
RSWRGSTLGWTTSWPSLV*SWSCGGCTQATCCP
TRSCSGCS*MRVAGWAPCAFCTPRCPSMCCSSA
PPWAPAATRGRR*YTGLVRQQLWSGGQTHGWWST
AGASSHPPWPSRWPTLSSAPRTSSPSSILFAPM
LGASGLSSPPTSLARTLDQPGLKEDLRIDRSGQ
ARTYPLAGAHVYRQGHTPCRS*VPAV*AGISMLM
YPNTETHGNK*DTYRY*DLCVQ*DHALTPIWRG
SPRYTKGASCVQTHTSQLDSLTEAFP*LHSFPP
PPHQTGVLELRMGEGIILPQSDSSTQQQFEGMRG
KRSCLLEAPFTCSYDALPLLPCPHHMPYPHSTP
LLCKCPCGLSPTPSATKLSWGTRVI*RMLEVSV
FHSRKTPILPLGV*CGSWFQPRTHH*GEDLDRWA
*FQGALPGLEKAFYTHTTHTHTHTHTHITVFTQ
PLLHSLSICLFLLIKIC*SVP
Frame 1
SEAVSAKPQAPAPS*AAPCQAARRDAVGRGPAVGVGRAAPGCRSGADPGRLALAGYAELRLPARRD
SAVGAAVRWAGPRAGLLSSDRGAAAAAPRPRAAR
RGAAVGVRECGWLDGRHVPSARLAVRVCAALRH
RLGLPRPLGGDGSTRAW*GNSCGVGAKHMDGGV
RPGRHPIHPGLRAGRHCLQHPGLPHPLLYSSLLC
SGPPA*AHHLPLWPGPLTSQA*RKTCG*TGAGR
PAHIHLLEPMFTDRDIHHADPEFLLYEQGYPCL
CIQTQRPMGTNETHIDTETCVYSRTMHSHPSGEG
APGIPREPVVFRHTHHSLTH*LRPFHSSTASHL
LPTKPGF*S*GWGRVLYCLSLTPQPSSNLRG*G
GRGAAFWRPPSPAAMMPFPFSPVLTICLIPILLP
CYASAPVACPQPPQQQSSAGERE*FEECLKSAS
SIPERPPFFLWGYDVEAGFSPGPTTEERI*TGG
PNSKGPFLAWRRPFTHTQHIHTHTHTHISQFSHS
PCCILCPSVCFY**RFVDLF
Frame 2
PRP*AQSLRPRLPPELRRARPPAGMQWAVGRRWAWAALLLAVAAVLTQVVWLWLGTQSFVFQREEI
AQLARQYAGLDHELAFSRLIVELRRLHPGHVLP
DEELQWVFVNAGGWMGAMCLLHASLSEYVLLFGT
ALGSRGHSGETVVHGPGEATAVEWGPNTWMVEY
GRGVIPSTLAFALADTVFSTQDFLTLFYTLRSY
ARGLRLELTTYLFGQDP*PARPEGRPADRQERAG
PHISTCWSPCLQTGTYTMQILSSCCMSRDIHAY
VSKHRDPWEQMRHI*ILRPVCTVGPCTHTHLER
EPPVYQGSQLCSDTHITA*LTN*GLSIAPQLPTS
SPPNRGSRVKDGGGYYTASV*LLNPAAI*GDEG
EEELPFGGPLHLQL*CPSPSPLSSPYALSPFYS
PAMQVPLWLVPNPLSNKAQLGNESNLKNA*SQRL
PFQKDPHSSFGGMMWKLVSAQDPPLRRGSRQVG
LIPRGPSWPGEGLLHTHNTYTHTHTHTYHSFHT
APAAFSVHLSVSINKDLLICS
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