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Sequences

Sequence NM_001587

TCTCAGCTCCCAGCTCCCCGCTCCCGGCTCCCGGCGCCCGGCGCCCGGCGCGGAGCTGTTCCTCAAACGACACGCAGCCGAGGTGGGTGGGTGTGGGGAC GCGGGAGCCAGTGTCGTCGGATCGGCCCGCAGTCCGCTGTCCTGCTGAGCCCGGAGGCCGCCTGGATGGAGCCGCCGCTCCCGGTCGGAGCCCAGCCGCT TGCCACTGTCGAGGGTATGGAGATGAAGGGTCCTCTCCGGGAGCCCTGCGCCCTGACCCTAGCCCAGAGGAACGGGCAATATGAGTTAATAATCCAGTTG CATGAGAAGGAACAGCATGTTCAAGATATCATTCCTATAAATAGCCACTTCAGATGTGTTCAAGAAGCAGAAGAAACTCTTTTGATTGACATAGCTTCTA ACAGTGGCTGCAAAATTCGGGTTCAGGGGGACTGGATCAGAGAGCGCCGCTTTGAAATCCCTGATGAGGAACACTGTTTGAAGTTCCTCTCAGCTGTCCT TGCTGCTCAGAAAGCTCAGTCACAGCTTCTTGTTCCAGAGCAAAAGGACTCATCTAGCTGGTACCAGAAATTAGACACTAAGGACAAACCTTCTGTTTTT TCAGGGCTTCTTGGATTTGAAGACAATTTTTCTTCTATGAATTTGGACAAGAAAATAAATTCACAAAATCAGCCTACTGGGATTCATCGGGAACCCCCAC CTCCACCCTTTTCAGTGAATAAAATGCTTCCACGTGAAAAAGAAGCTTCTAACAAGGAGCAGCCCAAAGTGACCAACACCATGCGGAAGCTCTTTGTACC AAATACCCAATCTGGGCAGCGGGAGGGTCTCATCAAACATATCCTGGCAAAGCGAGAGAAAGAATATGTCAACATTCAGACTTTCAGATTTTTTGTTGGA ACTTGGAATGTGAATGGCCAGTCTCCAGATAGCGGGTTAGAACCTTGGCTGAACTGTGATCCCAATCCTCCTGATATCTACTGCATTGGATTCCAAGAAC TGGACTTGAGCACAGAAGCCTTCTTCTACTTTGAATCTGTGAAGGAACAAGAATGGTCCATGGCTGTAGAGAGAGGTTTGCATTCCAAAGCCAAGTATAA GAAAGTTCAACTGGTGCGCCTTGTTGGGATGATGCTTCTTATATTTGCCAGAAAGGATCAGTGTCGATACATTCGTGATATTGCTACAGAAACAGTTGGA ACTGGAATCATGGGGAAAATGGGAAACAAAGGTGGGGTAGCTGTGAGATTTGTATTTCACAACACCACCTTTTGCATTGTCAATTCCCATCTGGCTGCAC ACGTGGAGGACTTTGAGAGAAGGAATCAAGATTATAAGGACATTTGTGCGAGAATGAGTTTTGTGGTCCCAAATCAGACCCTCCCGCAGTTGAACATCAT GAAACATGAGGTTGTCATTTGGTTGGGAGATTTGAATTATAGACTTTGCATGCCTGATGCCAATGAGGTGAAAAGTCTTATTAATAAGAAAGACCTTCAG AGACTCTTGAAATTCGACCAGCTAAATATTCAGCGCACACAGAAAAAAGCTTTTGTTGACTTCAATGAAGGGGAAATCAAGTTCATCCCCACTTATAAGT ATGACTCTAAAACAGACCGGTGGGATTCCAGTGGGAAATGCCGGGTTCCAGCCTGGTGTGACCGAATTCTTTGGAGAGGAACAAATGTTAATCAGCTTAA TTATCGGAGTCACATGGAACTGAAAACCAGCGACCACAAGCCTGTTAGCGCCCTCTTCCATATTGGGGTGAAGGTTGTGGATGAACGAAGGTACCGGAAA GTCTTTGAAGATAGTGTACGCATCATGGACAGAATGGAAAATGACTTCCTTCCTTCCTTAGAACTCAGCAGGAGGGAGTTTGTGTTTGAAAATGTGAAGT TTCGGCAACTACAAAAGGAGAAGTTCCAGATCAGCAACAATGGACAGGTTCCCTGCCATTTTTCTTTCATCCCTAAACTTAATGACAGCCAGTACTGCAA GCCATGGCTTCGGGCTGAACCTTTTGAGGGCTACTTGGAGCCAAATGAGACAGTGGACATTTCTCTTGATGTGTATGTCAGCAAAGACTCTGTAACCATC CTGAACTCGGGAGAAGATAAGATTGAAGATATTCTCGTCCTTCACCTGGATCGAGGCAAAGATTACTTCTTGACTATCAGTGGAAATTACCTCCCAAGTT GTTTTGGCACATCCTTAGAGGCTCTGTGCCGTATGAAAAGACCAATCCGAGAAGTTCCTGTTACCAAACTCATAGACTTGGAGAAATCCCTTCTGCAAAT GGTTCCTTTGGATGAAGGTGCCAGTGAGAGACCCCTTCAGGTTCCCAAGGAGATCTGGCTTCTAGTAGATCACCTATTCAAATACGCCTGTCACCAGGAG GACCTGTTCCAGACCCCTGGAATGCAGGAAGAGCTCCAGCAGATCATTGATTGTCTGGATACCAGCATTCCTGAGACAATCCCTGGCAGCAACCACTCTG TGGCTGAAGCACTGCTCATTTTCTTGGAAGCCCTGCCAGAGCCAGTCATCTGTTACGAGCTGTATCAGCGATGTCTTGACTCTGCTTATGATCCCCGGAT CTGCCGACAGGTGATCTCCCAGCTTCCGAGATGCCATAGAAATGTTTTCCGTTACTTGATGGCATTCCTTCGAGAACTCTTAAAATTCTCTGAATACAAT AGCGTCAATGCCAACATGATCGCTACTCTCTTCACTAGTCTTCTCCTGAGGCCTCCACCCAACCTTATGGCAAGACAGACTCCAAGTGACCGCCAGCGTG CTATTCAGTTCCTTCTGGGCTTTCTGCTTGGGAGCGAAGAAGACTAAGGCTTTTACTGTTCTCTGATATTCTAGAAGCAGACGATCTCGGGCTCCAAGTA TTTCAGAATGATTTAAAAAGTCATGCCACAGGAAGGGTCTATTGCAGAATTTCAAGTTCTGTTTATAGTAAAAAGGAAGAGCGTTTCCTAATCCCTCCTT TACCATATCCTACACAGAAAAATACTTTTAGACTTATATTGCCAAGCCAAAGTTACCATATTTTGGTGTTTTTGTGTTTTCTCTTTATAAGGCAAAAAGA TCTGTATTTACACTCCTTCACCTAGGGATGTGTTTGTTGCCCTCCTACCCAATTGTCATGATTGTCCTTAGTACCCTAGGCCTAGATTCTGAGATCTTCC CATTCTAGGCCTACAAGCACTACTTGCTGTAGCTGAGACTTGTCTAGAGTCCTTTGTTTTGCACTTTTGACCCACCCCTTCCTGGATCACTCCTTTGCAC TCCACTCCCCTCGTTCTGTCACTTTGAACGAAGTCTGAGTGAGGCTAGTGACTCCTTGGGTGTCCTCAACAGTGAATTCACTGTCTGCGTGCAGTTATTA CATGCATTTGTGCATTTCTACTACAATGGCATCTTTATGTCTCTGTAACATTGGCCTTTTCATGGCTCCACACTGGGTGGAACCATATTCTCTTAGATCA CATTTAGTAGCATAACTGTAGGGACTATTAGAGATGGCATCTCATCGATGAGAGAGAATCACAATCAGAATGGAAGCACTTTGAGTATCTGAAGAGTGAG AGCATTCATGTTTGACAGGTCCTGCTTCCCACTATCCTTTTCCTGTTATTATTCAAATTTTACACAAGGACTAATCCTGGGTGTCTCTGAGACCCATCTC CTGCCTAGACATCCACCTCCAGAGCAACACTGGCCCCACAGTAAAAGAGGAAGTCTTGTACCTCAGGCAGGCCCATCTAGAGCTATTGCTCCTTCCCACA GCAAAGGTATTGTGGATGACCCTTAGAATCCATTCTCTGGTCTTCTGAAATACCAAGGGCAGATGTCACCTCCTTCCTCAGCAGGACTGACTCTGGGCTC TACAACCAGCTCCTTCACATAAAGGGTTTAGAGACTCCCCTTGGCTCCCAGTCACCATATCCAGTGTTGTGTAAAGAGACTGGCCAACAGGACCAACCAA GCACCTTACCTCTCCCATACAAGATGACCCTCTGAGCTTTTCATTTATTCAAGCTCTGTGGTACAGCCTTTTTTTAAAATAAATTAATCTATATTGGTTG ACAAACAAGCCACCAACCACTGACTGCAAAACTGCCTGATGCAGTTGGGTTCCTCCTGGTTTTCTTTTGTTACAACCACCCTTGCCTGTTTACATTAATT GCAAGGAGCATAACGTACAGGCTGTATGTACAATCCTGGGCATTGACTCTGTGACATTTCTAGCATATCCAAGGCACCACCAGTGATTTCTCCTGTTTCT TGGTGGGGGTGGGGGGGAAGGTACGTATTCTGCAATATGGCTAAACCCTTTCCTGATTGAGAGTTAAAGCAATAGGAGTCAAGTTACTGGTGCCACAGAT CTGGAGGTATGATAGGTCAGGGGCTAGGTGTTGAACTTAGTTAATGGAAGACTGAGAGCAGAACAGGTTTGTCATCTCCGCAAGGCCAGAAAGTGATCAC AAAAAGAGGCAGATGATAGACACTGGGGTAGGGTCATACCACAGGGAAATACCTTTCCTGGGCTTGTTTTCTAGCATATCACTGACCTGGGATCTTTGGG TGATCAAGGGTGTGGTTAGTGGAGGCTCTGTGCTGCACGTATGCAGTATCCTATCTCTTTCTACATCAGATCAAAACACTAAGTTGGTGTACTGCCTCGA CCTTTTTTCAGCTCATCCTGGAACATATACAGAGTTGAGAGTTTTAGACAATCTCTAGGTAGAGGAGACAAGATGTAGACCCAGACAGAAGAAATCTGCT TCCCTACCATGGCTATTCCAGCACCCCAACCTGTAATTGCCAAGTCCTCTAAGGTACTAATTTGTAGCTGCTCTGAAGTAAGGATTTCGGATTCAGCTGG TAGGGAAAGACTCTGCACCTGCTGTCTTAGGGAAGAAATGGTTCAAATCCATGTGGTGACATTGCATTAGTCTCCCTTTCACTGTTTTCTTATTCTGTAA TTGTTTGTTATATTTCCCAAAAACGTCTTGATCACTAAGCAAAGCTGCTAGTGGGATTCTATATTTCGTGTCATCTTTTTTATTATAATTTATTGCAAAT TTTTTTCTGAATAAATATATGTTGTGTGAAAAA

refseq_OCRL.F1 (forward primer)
refseq_OCRL.R1 (reverse primer)
Single exon skipping

Frame 0

SQLPAPRSRLPAPGARRGAVPQTTRSRGGWVWG RGSQCRRIGPQSAVLLSPEAAWMEPPLPVGAQPL ATVEGMEMKGPLREPCALTLAQRNGQYELIIQL HEKEQHVQDIIPINSHFRCVQEAEETLLIDIAS NSGCKIRVQGDWIRERRFEIPDEEHCLKFLSAVL AAQKAQSQLLVPEQKDSSSWYQKLDTKDKPSVF SGLLGFEDNFSSMNLDKKINSQNQPTGIHREPP PPPFSVNKMLPREKEASNKEQPKVTNTMRKLFVP NTQSGQREGLIKHILAKREKEYVNIQTFRFFVG TWNVNGQSPDSGLEPWLNCDPNPPDIYCIGFQE LDLSTEAFFYFESVKEQEWSMAVERGLHSKAKYK KVQLVRLVGMMLLIFARKDQCRYIRDIATETVG TGIMGKMGNKGGVAVRFVFHNTTFCIVNSHLAA HVEDFERRNQDYKDICARMSFVVPNQTLPQLNIM KHEVVIWLGDLNYRLCMPDANEVKSLINKKDLQ RLLKFDQLNIQRTQKKAFVDFNEGEIKFIPTYK YDSKTDRWDSSGKCRVPAWCDRILWRGTNVNQLN YRSHMELKTSDHKPVSALFHIGVKVVDERRYRK VFEDSVRIMDRMENDFLPSLELSRREFVFENVK FRQLQKEKFQISNNGQVPCHFSFIPKLNDSQYCK PWLRAEPFEGYLEPNETVDISLDVYVSKDSVTI LNSGEDKIEDILVLHLDRGKDYFLTISGNYLPS CFGTSLEALCRMKRPIREVPVTKLIDLEKSLLQM VPLDEGASERPLQVPKEIWLLVDHLFKYACHQE DLFQTPGMQEELQQIIDCLDTSIPETIPGSNHS VAEALLIFLEALPEPVICYELYQRCLDSAYDPRI CRQVISQLPRCHRNVFRYLMAFLRELLKFSEYN SVNANMIATLFTSLLLRPPPNLMARQTPSDRQR AIQFLLGFLLGSEED*GFYCSLIF*KQTISGSKY FRMI*KVMPQEGSIAEFQVLFIVKRKSVS*SLL YHILHRKILLDLYCQAKVTIFWCFCVFSL*GKK ICIYTPSPRDVFVALLPNCHDCP*YPRPRF*DLP ILGLQALLAVAETCLESFVLHF*PTPSWITPLH STPLVLSL*TKSE*G**LLGCPQQ*IHCLRAVI TCICAFLLQWHLYVSVTLAFSWLHTGWNHILLDH I**HNCRDY*RWHLIDERESQSEWKHFEYLKSE SIHV*QVLLPTILFLLLFKFYTRTNPGCL*DPS PA*TSTSRATLAPQ*KRKSCTSGRPI*SYCSFPQ QRYCG*PLESILWSSEIPRADVTSFLSRTDSGL YNQLLHIKGLETPLGSQSPYPVLCKETGQQDQP STLPLPYKMTL*AFHLFKLCGTAFF*NKLIYIG* QTSHQPLTAKLPDAVGFLLVFFCYNHPCLFTLI ARSITYRLYVQSWALTL*HF*HIQGTTSDFSCF LVGVGGKVRILQYG*TLS*LRVKAIGVKLLVPQI WRYDRSGARC*T*LMED*EQNRFVISARPESDH KKRQMIDTGVGSYHREIPFLGLFSSISLTWDLW VIKGVVSGGSVLHVCSILSLSTSDQNTKLVYCLD LFSAHPGTYTELRVLDNL*VEETRCRPRQKKSA SLPWLFQHPNL*LPSPLRY*FVAALK*GFRIQL VGKDSAPAVLGKKWFKSMW*HCISLPFTVFLFCN CLLYFPKTS*SLSKAASGILYFVSSFLL*FIAN FFLNKYMLCEK

Frame 1

LSSQLPAPGSRRPAPGAELFLKRHAAEVGGCGD AGASVVGSARSPLSC*ARRPPGWSRRSRSEPSR LPLSRVWR*RVLSGSPAP*P*PRGTGNMS**SSC MRRNSMFKISFL*IATSDVFKKQKKLF*LT*LL TVAAKFGFRGTGSESAALKSLMRNTV*SSSQLS LLLRKLSHSFLFQSKRTHLAGTRN*TLRTNLLFF QGFLDLKTIFLL*IWTRK*IHKISLLGFIGNPH LHPFQ*IKCFHVKKKLLTRSSPK*PTPCGSSLY QIPNLGSGRVSSNISWQSERKNMSTFRLSDFLLE LGM*MASLQIAG*NLG*TVIPILLISTALDSKN WT*AQKPSSTLNL*RNKNGPWL*REVCIPKPSI RKFNWCALLG*CFLYLPERISVDTFVILLQKQLE LESWGKWETKVG*L*DLYFTTPPFALSIPIWLH TWRTLREGIKIIRTFVRE*VLWSQIRPSRS*TS *NMRLSFGWEI*IIDFACLMPMR*KVLLIRKTFR DS*NSTS*IFSAHRKKLLLTSMKGKSSSSPLIS MTLKQTGGIPVGNAGFQPGVTEFFGEEQMLISL IIGVTWN*KPATTSLLAPSSILG*RLWMNEGTGK SLKIVYASWTEWKMTSFLP*NSAGGSLCLKM*S FGNYKRRSSRSATMDRFPAIFLSSLNLMTASTA SHGFGLNLLRATWSQMRQWTFLLMCMSAKTL*PS *TREKIRLKIFSSFTWIEAKITS*LSVEITSQV VLAHP*RLCAV*KDQSEKFLLPNS*TWRNPFCK WFLWMKVPVRDPFRFPRRSGF**ITYSNTPVTRR TCSRPLECRKSSSRSLIVWIPAFLRQSLAATTL WLKHCSFSWKPCQSQSSVTSCISDVLTLLMIPG SADR*SPSFRDAIEMFSVT*WHSFENS*NSLNTI ASMPT*SLLSSLVFS*GLHPTLWQDRLQVTASV LFSSFWAFCLGAKKTKAFTVL*YSRSRRSRAPS ISE*FKKSCHRKGLLQNFKFCL**KGRAFPNPSF TISYTEKYF*TYIAKPKLPYFGVFVFSLYKAKR SVFTLLHLGMCLLPSYPIVMIVLSTLGLDSEIF PF*AYKHYLL*LRLV*SPLFCTFDPPLPGSLLCT PLPSFCHFERSLSEASDSLGVLNSEFTVCVQLL HAFVHFYYNGIFMSL*HWPFHGSTLGGTIFS*I TFSSITVGTIRDGISSMRENHNQNGSTLSI*RVR AFMFDRSCFPLSFSCYYSNFTQGLILGVSETHL LPRHPPPEQHWPHSKRGSLVPQAGPSRAIAPSH SKGIVDDP*NPFSGLLKYQGQMSPPSSAGLTLGS TTSSFT*RV*RLPLAPSHHIQCCVKRLANRTNQ APYLSHTR*PSELFIYSSSVVQPFFKIN*SILV DKQATNH*LQNCLMQLGSSWFSFVTTTLACLH*L QGA*RTGCMYNPGH*LCDISSISKAPPVISPVS WWGWGGRYVFCNMAKPFPD*ELKQ*ESSYWCHR SGGMIGQGLGVELS*WKTESRTGLSSPQGQKVIT KRGR**TLG*GHTTGKYLSWACFLAYH*PGIFG *SRVWLVEALCCTYAVSYLFLHQIKTLSWCTAS TFFQLILEHIQS*EF*TISR*RRQDVDPDRRNLL PYHGYSSTPTCNCQVL*GTNL*LL*SKDFGFSW *GKTLHLLS*GRNGSNPCGDIALVSLSLFSYSV IVCYISQKRLDH*AKLLVGFYISCHLFYYNLLQI FF*INICCVK

Frame 2

SAPSSPLPAPGARRPARSCSSNDTQPRWVGVGT REPVSSDRPAVRCPAEPGGRLDGAAAPGRSPAA CHCRGYGDEGSSPGALRPDPSPEERAI*VNNPV A*EGTACSRYHSYK*PLQMCSRSRRNSFD*HSF* QWLQNSGSGGLDQRAPL*NP**GTLFEVPLSCP CCSESSVTASCSRAKGLI*LVPEIRH*GQTFCF FRASWI*RQFFFYEFGQENKFTKSAYWDSSGTPT STLFSE*NAST*KRSF*QGAAQSDQHHAEALCT KYPIWAAGGSHQTYPGKARERICQHSDFQIFCW NLECEWPVSR*RVRTLAEL*SQSS*YLLHWIPRT GLEHRSLLLL*ICEGTRMVHGCRERFAFQSQV* ESSTGAPCWDDASYICQKGSVSIHS*YCYRNSW NWNHGENGKQRWGSCEICISQHHLLHCQFPSGCT RGGL*EKESRL*GHLCENEFCGPKSDPPAVEHH ET*GCHLVGRFEL*TLHA*CQ*GEKSY**ERPS ETLEIRPAKYSAHTEKSFC*LQ*RGNQVHPHL*V *L*NRPVGFQWEMPGSSLV*PNSLERNKC*SA* LSESHGTENQRPQAC*RPLPYWGEGCG*TKVPE SL*R*CTHHGQNGK*LPSFLRTQQEGVCV*KCEV SATTKGEVPDQQQWTGSLPFFFHP*T**QPVLQ AMASG*TF*GLLGAK*DSGHFS*CVCQQRLCNH PELGRR*D*RYSRPSPGSRQRLLLDYQWKLPPKL FWHILRGSVPYEKTNPRSSCYQTHRLGEIPSAN GSFG*RCQ*ETPSGSQGDLASSRSPIQIRLSPG GPVPDPWNAGRAPADH*LSGYQHS*DNPWQQPLC G*STAHFLGSPARASHLLRAVSAMS*LCL*SPD LPTGDLPASEMP*KCFPLLDGIPSRTLKIL*IQ *RQCQHDRYSLH*SSPEASTQPYGKTDSK*PPAC YSVPSGLSAWERRRLRLLLFSDILEADDLGLQV FQNDLKSHATGRVYCRISSSVYSKKEERFLIPP LPYPTQKNTFRLILPSQSYHILVFLCFLFIRQKD LYLHSFT*GCVCCPPTQLS*LSLVP*A*ILRSS HSRPTSTTCCS*DLSRVLCFALLTHPFLDHSFA LHSPRSVTLNEV*VRLVTPWVSSTVNSLSACSYY MHLCISTTMASLCLCNIGLFMAPHWVEPYSLRS HLVA*L*GLLEMASHR*ERITIRMEAL*VSEE* EHSCLTGPASHYPFPVIIQILHKD*SWVSLRPIS CLDIHLQSNTGPTVKEEVLYLRQAHLELLLLPT AKVLWMTLRIHSLVF*NTKGRCHLLPQQD*LWA LQPAPSHKGFRDSPWLPVTISSVV*RDWPTGPTK HLTSPIQDDPLSFSFIQALWYSLFLK*INLYWL TNKPPTTDCKTA*CSWVPPGFLLLQPPLPVYIN CKEHNVQAVCTILGIDSVTFLAYPRHHQ*FLLFL GGGGGEGTYSAIWLNPFLIES*SNRSQVTGATD LEV**VRG*VLNLVNGRLRAEQVCHLRKARK*S QKEADDRHWGRVIPQGNTFPGLVF*HITDLGSLG DQGCG*WRLCAARMQYPISFYIRSKH*VGVLPR PFFSSSWNIYRVESFRQSLGRGDKM*TQTEEIC FPTMAIPAPQPVIAKSSKVLICSCSEVRISDSAG RERLCTCCLREEMVQIHVVTLH*SPFHCFLIL* LFVIFPKNVLITKQSC*WDSIFRVIFFIIIYCK FFSE*IYVV*K

Sequence NM_000276

TCTCAGCTCCCAGCTCCCCGCTCCCGGCTCCCGGCGCCCGGCGCCCGGCGCGGAGCTGTTCCTCAAACGACACGCAGCCGAGGTGGGTGGGTGTGGGGAC GCGGGAGCCAGTGTCGTCGGATCGGCCCGCAGTCCGCTGTCCTGCTGAGCCCGGAGGCCGCCTGGATGGAGCCGCCGCTCCCGGTCGGAGCCCAGCCGCT TGCCACTGTCGAGGGTATGGAGATGAAGGGTCCTCTCCGGGAGCCCTGCGCCCTGACCCTAGCCCAGAGGAACGGGCAATATGAGTTAATAATCCAGTTG CATGAGAAGGAACAGCATGTTCAAGATATCATTCCTATAAATAGCCACTTCAGATGTGTTCAAGAAGCAGAAGAAACTCTTTTGATTGACATAGCTTCTA ACAGTGGCTGCAAAATTCGGGTTCAGGGGGACTGGATCAGAGAGCGCCGCTTTGAAATCCCTGATGAGGAACACTGTTTGAAGTTCCTCTCAGCTGTCCT TGCTGCTCAGAAAGCTCAGTCACAGCTTCTTGTTCCAGAGCAAAAGGACTCATCTAGCTGGTACCAGAAATTAGACACTAAGGACAAACCTTCTGTTTTT TCAGGGCTTCTTGGATTTGAAGACAATTTTTCTTCTATGAATTTGGACAAGAAAATAAATTCACAAAATCAGCCTACTGGGATTCATCGGGAACCCCCAC CTCCACCCTTTTCAGTGAATAAAATGCTTCCACGTGAAAAAGAAGCTTCTAACAAGGAGCAGCCCAAAGTGACCAACACCATGCGGAAGCTCTTTGTACC AAATACCCAATCTGGGCAGCGGGAGGGTCTCATCAAACATATCCTGGCAAAGCGAGAGAAAGAATATGTCAACATTCAGACTTTCAGATTTTTTGTTGGA ACTTGGAATGTGAATGGCCAGTCTCCAGATAGCGGGTTAGAACCTTGGCTGAACTGTGATCCCAATCCTCCTGATATCTACTGCATTGGATTCCAAGAAC TGGACTTGAGCACAGAAGCCTTCTTCTACTTTGAATCTGTGAAGGAACAAGAATGGTCCATGGCTGTAGAGAGAGGTTTGCATTCCAAAGCCAAGTATAA GAAAGTTCAACTGGTGCGCCTTGTTGGGATGATGCTTCTTATATTTGCCAGAAAGGATCAGTGTCGATACATTCGTGATATTGCTACAGAAACAGTTGGA ACTGGAATCATGGGGAAAATGGGAAACAAAGGTGGGGTAGCTGTGAGATTTGTATTTCACAACACCACCTTTTGCATTGTCAATTCCCATCTGGCTGCAC ACGTGGAGGACTTTGAGAGAAGGAATCAAGATTATAAGGACATTTGTGCGAGAATGAGTTTTGTGGTCCCAAATCAGACCCTCCCGCAGTTGAACATCAT GAAACATGAGGTTGTCATTTGGTTGGGAGATTTGAATTATAGACTTTGCATGCCTGATGCCAATGAGGTGAAAAGTCTTATTAATAAGAAAGACCTTCAG AGACTCTTGAAATTCGACCAGCTAAATATTCAGCGCACACAGAAAAAAGCTTTTGTTGACTTCAATGAAGGGGAAATCAAGTTCATCCCCACTTATAAGT ATGACTCTAAAACAGACCGGTGGGATTCCAGTGGGAAATGCCGGGTTCCAGCCTGGTGTGACCGAATTCTTTGGAGAGGAACAAATGTTAATCAGCTTAA TTATCGGAGTCACATGGAACTGAAAACCAGCGACCACAAGCCTGTTAGCGCCCTCTTCCATATTGGGGTGAAGGTTGTGGATGAACGAAGGTACCGGAAA GTCTTTGAAGATAGTGTACGCATCATGGACAGAATGGAAAATGACTTCCTTCCTTCCTTAGAACTCAGCAGGAGGGAGTTTGTGTTTGAAAATGTGAAGT TTCGGCAACTACAAAAGGAGAAGTTCCAGATCAGCAACAATGGACAGGTTCCCTGCCATTTTTCTTTCATCCCTAAACTTAATGACAGCCAGTACTGCAA GCCATGGCTTCGGGCTGAACCTTTTGAGGGCTACTTGGAGCCAAATGAGACAGTGGACATTTCTCTTGATGTGTATGTCAGCAAAGACTCTGTAACCATC CTGAACTCGGGAGAAGATAAGATTGAAGATATTCTCGTCCTTCACCTGGATCGAGGCAAAGATTACTTCTTGACTATCAGTGGAAATTACCTCCCAAGTT GTTTTGGCACATCCTTAGAGGCTCTGTGCCGTATGAAAAGACCAATCCGAGAAGTTCCTGTTACCAAACTCATAGACTTGGAAGAAGACAGCTTCCTAGA AAAGGAGAAATCCCTTCTGCAAATGGTTCCTTTGGATGAAGGTGCCAGTGAGAGACCCCTTCAGGTTCCCAAGGAGATCTGGCTTCTAGTAGATCACCTA TTCAAATACGCCTGTCACCAGGAGGACCTGTTCCAGACCCCTGGAATGCAGGAAGAGCTCCAGCAGATCATTGATTGTCTGGATACCAGCATTCCTGAGA CAATCCCTGGCAGCAACCACTCTGTGGCTGAAGCACTGCTCATTTTCTTGGAAGCCCTGCCAGAGCCAGTCATCTGTTACGAGCTGTATCAGCGATGTCT TGACTCTGCTTATGATCCCCGGATCTGCCGACAGGTGATCTCCCAGCTTCCGAGATGCCATAGAAATGTTTTCCGTTACTTGATGGCATTCCTTCGAGAA CTCTTAAAATTCTCTGAATACAATAGCGTCAATGCCAACATGATCGCTACTCTCTTCACTAGTCTTCTCCTGAGGCCTCCACCCAACCTTATGGCAAGAC AGACTCCAAGTGACCGCCAGCGTGCTATTCAGTTCCTTCTGGGCTTTCTGCTTGGGAGCGAAGAAGACTAAGGCTTTTACTGTTCTCTGATATTCTAGAA GCAGACGATCTCGGGCTCCAAGTATTTCAGAATGATTTAAAAAGTCATGCCACAGGAAGGGTCTATTGCAGAATTTCAAGTTCTGTTTATAGTAAAAAGG AAGAGCGTTTCCTAATCCCTCCTTTACCATATCCTACACAGAAAAATACTTTTAGACTTATATTGCCAAGCCAAAGTTACCATATTTTGGTGTTTTTGTG TTTTCTCTTTATAAGGCAAAAAGATCTGTATTTACACTCCTTCACCTAGGGATGTGTTTGTTGCCCTCCTACCCAATTGTCATGATTGTCCTTAGTACCC TAGGCCTAGATTCTGAGATCTTCCCATTCTAGGCCTACAAGCACTACTTGCTGTAGCTGAGACTTGTCTAGAGTCCTTTGTTTTGCACTTTTGACCCACC CCTTCCTGGATCACTCCTTTGCACTCCACTCCCCTCGTTCTGTCACTTTGAACGAAGTCTGAGTGAGGCTAGTGACTCCTTGGGTGTCCTCAACAGTGAA TTCACTGTCTGCGTGCAGTTATTACATGCATTTGTGCATTTCTACTACAATGGCATCTTTATGTCTCTGTAACATTGGCCTTTTCATGGCTCCACACTGG GTGGAACCATATTCTCTTAGATCACATTTAGTAGCATAACTGTAGGGACTATTAGAGATGGCATCTCATCGATGAGAGAGAATCACAATCAGAATGGAAG CACTTTGAGTATCTGAAGAGTGAGAGCATTCATGTTTGACAGGTCCTGCTTCCCACTATCCTTTTCCTGTTATTATTCAAATTTTACACAAGGACTAATC CTGGGTGTCTCTGAGACCCATCTCCTGCCTAGACATCCACCTCCAGAGCAACACTGGCCCCACAGTAAAAGAGGAAGTCTTGTACCTCAGGCAGGCCCAT CTAGAGCTATTGCTCCTTCCCACAGCAAAGGTATTGTGGATGACCCTTAGAATCCATTCTCTGGTCTTCTGAAATACCAAGGGCAGATGTCACCTCCTTC CTCAGCAGGACTGACTCTGGGCTCTACAACCAGCTCCTTCACATAAAGGGTTTAGAGACTCCCCTTGGCTCCCAGTCACCATATCCAGTGTTGTGTAAAG AGACTGGCCAACAGGACCAACCAAGCACCTTACCTCTCCCATACAAGATGACCCTCTGAGCTTTTCATTTATTCAAGCTCTGTGGTACAGCCTTTTTTTA AAATAAATTAATCTATATTGGTTGACAAACAAGCCACCAACCACTGACTGCAAAACTGCCTGATGCAGTTGGGTTCCTCCTGGTTTTCTTTTGTTACAAC CACCCTTGCCTGTTTACATTAATTGCAAGGAGCATAACGTACAGGCTGTATGTACAATCCTGGGCATTGACTCTGTGACATTTCTAGCATATCCAAGGCA CCACCAGTGATTTCTCCTGTTTCTTGGTGGGGGTGGGGGGGAAGGTACGTATTCTGCAATATGGCTAAACCCTTTCCTGATTGAGAGTTAAAGCAATAGG AGTCAAGTTACTGGTGCCACAGATCTGGAGGTATGATAGGTCAGGGGCTAGGTGTTGAACTTAGTTAATGGAAGACTGAGAGCAGAACAGGTTTGTCATC TCCGCAAGGCCAGAAAGTGATCACAAAAAGAGGCAGATGATAGACACTGGGGTAGGGTCATACCACAGGGAAATACCTTTCCTGGGCTTGTTTTCTAGCA TATCACTGACCTGGGATCTTTGGGTGATCAAGGGTGTGGTTAGTGGAGGCTCTGTGCTGCACGTATGCAGTATCCTATCTCTTTCTACATCAGATCAAAA CACTAAGTTGGTGTACTGCCTCGACCTTTTTTCAGCTCATCCTGGAACATATACAGAGTTGAGAGTTTTAGACAATCTCTAGGTAGAGGAGACAAGATGT AGACCCAGACAGAAGAAATCTGCTTCCCTACCATGGCTATTCCAGCACCCCAACCTGTAATTGCCAAGTCCTCTAAGGTACTAATTTGTAGCTGCTCTGA AGTAAGGATTTCGGATTCAGCTGGTAGGGAAAGACTCTGCACCTGCTGTCTTAGGGAAGAAATGGTTCAAATCCATGTGGTGACATTGCATTAGTCTCCC TTTCACTGTTTTCTTATTCTGTAATTGTTTGTTATATTTCCCAAAAACGTCTTGATCACTAAGCAAAGCTGCTAGTGGGATTCTATATTTCGTGTCATCT TTTTTATTATAATTTATTGCAAATTTTTTTCTGAATAAATATATGTTGTGTGAAAAA

refseq_OCRL.F1 (forward primer)
refseq_OCRL.R1 (reverse primer)
Single exon skipping

Frame 0

SQLPAPRSRLPAPGARRGAVPQTTRSRGGWVWG RGSQCRRIGPQSAVLLSPEAAWMEPPLPVGAQPL ATVEGMEMKGPLREPCALTLAQRNGQYELIIQL HEKEQHVQDIIPINSHFRCVQEAEETLLIDIAS NSGCKIRVQGDWIRERRFEIPDEEHCLKFLSAVL AAQKAQSQLLVPEQKDSSSWYQKLDTKDKPSVF SGLLGFEDNFSSMNLDKKINSQNQPTGIHREPP PPPFSVNKMLPREKEASNKEQPKVTNTMRKLFVP NTQSGQREGLIKHILAKREKEYVNIQTFRFFVG TWNVNGQSPDSGLEPWLNCDPNPPDIYCIGFQE LDLSTEAFFYFESVKEQEWSMAVERGLHSKAKYK KVQLVRLVGMMLLIFARKDQCRYIRDIATETVG TGIMGKMGNKGGVAVRFVFHNTTFCIVNSHLAA HVEDFERRNQDYKDICARMSFVVPNQTLPQLNIM KHEVVIWLGDLNYRLCMPDANEVKSLINKKDLQ RLLKFDQLNIQRTQKKAFVDFNEGEIKFIPTYK YDSKTDRWDSSGKCRVPAWCDRILWRGTNVNQLN YRSHMELKTSDHKPVSALFHIGVKVVDERRYRK VFEDSVRIMDRMENDFLPSLELSRREFVFENVK FRQLQKEKFQISNNGQVPCHFSFIPKLNDSQYCK PWLRAEPFEGYLEPNETVDISLDVYVSKDSVTI LNSGEDKIEDILVLHLDRGKDYFLTISGNYLPS CFGTSLEALCRMKRPIREVPVTKLIDLEEDSFLE KEKSLLQMVPLDEGASERPLQVPKEIWLLVDHL FKYACHQEDLFQTPGMQEELQQIIDCLDTSIPE TIPGSNHSVAEALLIFLEALPEPVICYELYQRCL DSAYDPRICRQVISQLPRCHRNVFRYLMAFLRE LLKFSEYNSVNANMIATLFTSLLLRPPPNLMAR QTPSDRQRAIQFLLGFLLGSEED*GFYCSLIF*K QTISGSKYFRMI*KVMPQEGSIAEFQVLFIVKR KSVS*SLLYHILHRKILLDLYCQAKVTIFWCFC VFSL*GKKICIYTPSPRDVFVALLPNCHDCP*YP RPRF*DLPILGLQALLAVAETCLESFVLHF*PT PSWITPLHSTPLVLSL*TKSE*G**LLGCPQQ* IHCLRAVITCICAFLLQWHLYVSVTLAFSWLHTG WNHILLDHI**HNCRDY*RWHLIDERESQSEWK HFEYLKSESIHV*QVLLPTILFLLLFKFYTRTN PGCL*DPSPA*TSTSRATLAPQ*KRKSCTSGRPI *SYCSFPQQRYCG*PLESILWSSEIPRADVTSF LSRTDSGLYNQLLHIKGLETPLGSQSPYPVLCK ETGQQDQPSTLPLPYKMTL*AFHLFKLCGTAFF* NKLIYIG*QTSHQPLTAKLPDAVGFLLVFFCYN HPCLFTLIARSITYRLYVQSWALTL*HF*HIQG TTSDFSCFLVGVGGKVRILQYG*TLS*LRVKAIG VKLLVPQIWRYDRSGARC*T*LMED*EQNRFVI SARPESDHKKRQMIDTGVGSYHREIPFLGLFSS ISLTWDLWVIKGVVSGGSVLHVCSILSLSTSDQN TKLVYCLDLFSAHPGTYTELRVLDNL*VEETRC RPRQKKSASLPWLFQHPNL*LPSPLRY*FVAAL K*GFRIQLVGKDSAPAVLGKKWFKSMW*HCISLP FTVFLFCNCLLYFPKTS*SLSKAASGILYFVSS FLL*FIANFFLNKYMLCEK

Frame 1

LSSQLPAPGSRRPAPGAELFLKRHAAEVGGCGD AGASVVGSARSPLSC*ARRPPGWSRRSRSEPSR LPLSRVWR*RVLSGSPAP*P*PRGTGNMS**SSC MRRNSMFKISFL*IATSDVFKKQKKLF*LT*LL TVAAKFGFRGTGSESAALKSLMRNTV*SSSQLS LLLRKLSHSFLFQSKRTHLAGTRN*TLRTNLLFF QGFLDLKTIFLL*IWTRK*IHKISLLGFIGNPH LHPFQ*IKCFHVKKKLLTRSSPK*PTPCGSSLY QIPNLGSGRVSSNISWQSERKNMSTFRLSDFLLE LGM*MASLQIAG*NLG*TVIPILLISTALDSKN WT*AQKPSSTLNL*RNKNGPWL*REVCIPKPSI RKFNWCALLG*CFLYLPERISVDTFVILLQKQLE LESWGKWETKVG*L*DLYFTTPPFALSIPIWLH TWRTLREGIKIIRTFVRE*VLWSQIRPSRS*TS *NMRLSFGWEI*IIDFACLMPMR*KVLLIRKTFR DS*NSTS*IFSAHRKKLLLTSMKGKSSSSPLIS MTLKQTGGIPVGNAGFQPGVTEFFGEEQMLISL IIGVTWN*KPATTSLLAPSSILG*RLWMNEGTGK SLKIVYASWTEWKMTSFLP*NSAGGSLCLKM*S FGNYKRRSSRSATMDRFPAIFLSSLNLMTASTA SHGFGLNLLRATWSQMRQWTFLLMCMSAKTL*PS *TREKIRLKIFSSFTWIEAKITS*LSVEITSQV VLAHP*RLCAV*KDQSEKFLLPNS*TWKKTAS* KRRNPFCKWFLWMKVPVRDPFRFPRRSGF**ITY SNTPVTRRTCSRPLECRKSSSRSLIVWIPAFLR QSLAATTLWLKHCSFSWKPCQSQSSVTSCISDV LTLLMIPGSADR*SPSFRDAIEMFSVT*WHSFEN S*NSLNTIASMPT*SLLSSLVFS*GLHPTLWQD RLQVTASVLFSSFWAFCLGAKKTKAFTVL*YSR SRRSRAPSISE*FKKSCHRKGLLQNFKFCL**KG RAFPNPSFTISYTEKYF*TYIAKPKLPYFGVFV FSLYKAKRSVFTLLHLGMCLLPSYPIVMIVLST LGLDSEIFPF*AYKHYLL*LRLV*SPLFCTFDPP LPGSLLCTPLPSFCHFERSLSEASDSLGVLNSE FTVCVQLLHAFVHFYYNGIFMSL*HWPFHGSTL GGTIFS*ITFSSITVGTIRDGISSMRENHNQNGS TLSI*RVRAFMFDRSCFPLSFSCYYSNFTQGLI LGVSETHLLPRHPPPEQHWPHSKRGSLVPQAGP SRAIAPSHSKGIVDDP*NPFSGLLKYQGQMSPPS SAGLTLGSTTSSFT*RV*RLPLAPSHHIQCCVK RLANRTNQAPYLSHTR*PSELFIYSSSVVQPFF KIN*SILVDKQATNH*LQNCLMQLGSSWFSFVTT TLACLH*LQGA*RTGCMYNPGH*LCDISSISKA PPVISPVSWWGWGGRYVFCNMAKPFPD*ELKQ* ESSYWCHRSGGMIGQGLGVELS*WKTESRTGLSS PQGQKVITKRGR**TLG*GHTTGKYLSWACFLA YH*PGIFG*SRVWLVEALCCTYAVSYLFLHQIK TLSWCTASTFFQLILEHIQS*EF*TISR*RRQDV DPDRRNLLPYHGYSSTPTCNCQVL*GTNL*LL* SKDFGFSW*GKTLHLLS*GRNGSNPCGDIALVS LSLFSYSVIVCYISQKRLDH*AKLLVGFYISCHL FYYNLLQIFF*INICCVK

Frame 2

SAPSSPLPAPGARRPARSCSSNDTQPRWVGVGT REPVSSDRPAVRCPAEPGGRLDGAAAPGRSPAA CHCRGYGDEGSSPGALRPDPSPEERAI*VNNPV A*EGTACSRYHSYK*PLQMCSRSRRNSFD*HSF* QWLQNSGSGGLDQRAPL*NP**GTLFEVPLSCP CCSESSVTASCSRAKGLI*LVPEIRH*GQTFCF FRASWI*RQFFFYEFGQENKFTKSAYWDSSGTPT STLFSE*NAST*KRSF*QGAAQSDQHHAEALCT KYPIWAAGGSHQTYPGKARERICQHSDFQIFCW NLECEWPVSR*RVRTLAEL*SQSS*YLLHWIPRT GLEHRSLLLL*ICEGTRMVHGCRERFAFQSQV* ESSTGAPCWDDASYICQKGSVSIHS*YCYRNSW NWNHGENGKQRWGSCEICISQHHLLHCQFPSGCT RGGL*EKESRL*GHLCENEFCGPKSDPPAVEHH ET*GCHLVGRFEL*TLHA*CQ*GEKSY**ERPS ETLEIRPAKYSAHTEKSFC*LQ*RGNQVHPHL*V *L*NRPVGFQWEMPGSSLV*PNSLERNKC*SA* LSESHGTENQRPQAC*RPLPYWGEGCG*TKVPE SL*R*CTHHGQNGK*LPSFLRTQQEGVCV*KCEV SATTKGEVPDQQQWTGSLPFFFHP*T**QPVLQ AMASG*TF*GLLGAK*DSGHFS*CVCQQRLCNH PELGRR*D*RYSRPSPGSRQRLLLDYQWKLPPKL FWHILRGSVPYEKTNPRSSCYQTHRLGRRQLPR KGEIPSANGSFG*RCQ*ETPSGSQGDLASSRSP IQIRLSPGGPVPDPWNAGRAPADH*LSGYQHS*D NPWQQPLCG*STAHFLGSPARASHLLRAVSAMS *LCL*SPDLPTGDLPASEMP*KCFPLLDGIPSR TLKIL*IQ*RQCQHDRYSLH*SSPEASTQPYGKT DSK*PPACYSVPSGLSAWERRRLRLLLFSDILE ADDLGLQVFQNDLKSHATGRVYCRISSSVYSKK EERFLIPPLPYPTQKNTFRLILPSQSYHILVFLC FLFIRQKDLYLHSFT*GCVCCPPTQLS*LSLVP *A*ILRSSHSRPTSTTCCS*DLSRVLCFALLTH PFLDHSFALHSPRSVTLNEV*VRLVTPWVSSTVN SLSACSYYMHLCISTTMASLCLCNIGLFMAPHW VEPYSLRSHLVA*L*GLLEMASHR*ERITIRME AL*VSEE*EHSCLTGPASHYPFPVIIQILHKD*S WVSLRPISCLDIHLQSNTGPTVKEEVLYLRQAH LELLLLPTAKVLWMTLRIHSLVF*NTKGRCHLL PQQD*LWALQPAPSHKGFRDSPWLPVTISSVV*R DWPTGPTKHLTSPIQDDPLSFSFIQALWYSLFL K*INLYWLTNKPPTTDCKTA*CSWVPPGFLLLQ PPLPVYINCKEHNVQAVCTILGIDSVTFLAYPRH HQ*FLLFLGGGGGEGTYSAIWLNPFLIES*SNR SQVTGATDLEV**VRG*VLNLVNGRLRAEQVCH LRKARK*SQKEADDRHWGRVIPQGNTFPGLVF*H ITDLGSLGDQGCG*WRLCAARMQYPISFYIRSK H*VGVLPRPFFSSSWNIYRVESFRQSLGRGDKM *TQTEEICFPTMAIPAPQPVIAKSSKVLICSCSE VRISDSAGRERLCTCCLREEMVQIHVVTLH*SP FHCFLIL*LFVIFPKNVLITKQSC*WDSIFRVI FFIIIYCKFFSE*IYVV*K