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Sequences

Sequence NM_002606

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGTAATCTTCAGCAAGTACTGCAACTCCAGCGACATCATGGACCTGTTCTGCATCGCCACCGGCCTGCC
TCGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGAATTCAGAACGCACTCCGTACAAAGTGAGA
CCTGTGGCCATCAAGCAACTCTCCGCTGGTGTCGAGGACAAGAGAACCACAAGCCGTGGCCAGTCTGCTGAGAGACCACTGAGGGACAGACGGGTTGTGG
GCCTGGAGCAGCCCCGGAGGGAAGGAGCATTTGAAAGTGGACAGGTAGAGCCCAGGCCCAGAGAGCCCCAGGGCTGCTACCAGGAAGGCCAGCGCATCCC
TCCAGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCAGTTCTCAAGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAAT
CACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGG
CGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTA
CCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTAC
CACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACA
ACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAAT
GACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCA
CCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCC
GACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAA
CGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGT
TTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCC
AGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATC
CCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGA
GAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGAT
CCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCA
TGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F1 (forward primer)
  • refseq_PDE9A.R9 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKVIFSKYCNSSDIMDLFCIATGLP
RNTTISLLTTDDAMVSIDPTMPANSERTPYKVR
PVAIKQLSAGVEDKRTTSRGQSAERPLRDRRVV
GLEQPRREGAFESGQVEPRPREPQGCYQEGQRIP
PEREELIQSVLAQVAEQFSRAFKINELKAEVAN
HLAVLEKRVELEGLKVVEIEKCKSDIKKMREEL
AARSSRTNCPCKYSFLDNHKKLTPRRDVPTYPKY
LLSPETIEALRKPTFDVWLWEPNEMLSCLEHMY
HDLGLVRDFSINPVTLRRWLFCVHDNYRNNPFH
NFRHCFCVAQMMYSMVWLCSLQEKFSQTDILILM
TAAICHDLDHPGYNNTYQINARTELAVRYNDIS
PLENHHCAVAFQILAEPECNIFSNIPPDGFKQI
RQGMITLILATDMARHAEIMDSFKEKMENFDYSN
EEHMTLLKMILIKCCDISNEVRPMEVAEPWVDC
LLEEYFMQSDREKSEGLPVAPFMDRDKVTKATA
QIGFIKFVLIPMFETVTKLFPMVEEIMLQPLWES
RDRYEELKRIDDAMKELQKKTDSLTSGATEKSR
ERSRDVKNSEGDCA*GKRGAWLQFWTGWPSCAG
SLCREELPWAPGTTRPCFLRTILFTDTKKKKGIH
DAVQNFIFKLSFK*YILIRK

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRR*SSASTATPATSWTCSASPPAC
LGTRPSPC*PPTTPWSPSTPPCPRIQNALRTK*D
LWPSSNSPLVSRTREPQAVASLLRDH*GTDGLW
AWSSPGGKEHLKVDR*SPGPESPRAATRKASAS
LQREKN*SRACWRRLQSSSQEHSKSMN*KLKLQI
TWLS*RNAWNWKD*KWWRLRNARVTLRR*GRSW
RPEAAGPTAPVSTVFWITTRS*LLDAMFPLTPS
TCSLQRPSRPCGSRPLTSGFGSPMRC*AAWSTCT
TTSGWSGTSASTLSPSGGGCSASTTTTETTPST
TSGTASAWPR*CTAWSGSAVSRRSSHKRIS*S*
*QRPSATIWTIPATTTRTRSMPAQSWRSATMTSH
RWRTTTAPWPSRSSPSLSATSSPTSHLMGSSRS
DRE*SH*SWPLTWQDMQKLWILSKRKWRILTTA
TRST*PC*R*F**NAVISLTRSVQWKSQSLGWTV
Y*RNILCRATVRSQKAFLWHRSWTETK*PRPQP
RLGSSSLS*SQCLKQ*PSSSPWLRRSCCSHFGN
PEIATRS*SG*MTP*KSYRRRLTA*RLGPPRSPE
REAEM*KTVKETVPEESGGRGCSSGRAGRAARD
PCAGKSCPGHLAPQDHVF*EPFCSLIQKKKKEF
MMLYRILFLNCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGNLQQVLQLQRHHGPVLHRHRPA
SEHDHLPADHRRRHGLHRPHHAREFRTHSVQSE
TCGHQATLRWCRGQENHKPWPVC*ETTEGQTGCG
PGAAPEGRSI*KWTGRAQAQRAPGLLPGRPAHP
SRERRINPERAGAGCRAVLKSIQNQ*TES*SCK
SLGCPRETRGIGRTKSGGD*EMQE*H*EDEGGAG
GQKQQDQLPL*VQFFG*PQEVDSSTRCSHLPQV
PALSRDHRGPAEADL*RLALGAQ*DAELPGAHV
PRPRAGQGLQHQPCHPQEVAVLRPRQLQKQPLPQ
LPALLLRGPDDVQHGLALQSPGEVLTNGYPDPN
DSGHLPRSGPSRLQQHVPDQCPHRAGGPLQ*HL
TAGEPPLRRGLPDPRRA*VQHLLQHPT*WVQADP
TGNDHINLGH*HGKTCRNYGFFQRENGEF*LQQ
RGAHDPAEDDFDKML*YL*RGPSNGSRRALGGL
FIRGIFYAERP*EVRRPSCGTVHGPRQSDQGHSP
DWVHQVCPDPNV*NSDQALPHG*GDHAAATLGI
PRSLRGAEADR*RHERVTEED*QLDVWGHREVQ
REKQRCEKQ*RRLCLRKAGGVAAVLDGLAELRGI
LVQGRAALGTWHHKTMFSKNHFVH*YKKKKRNS
*CCTEFYF*TVF*IIYSYTE

Sequence NM_001001567

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGTAATCTTCAGCAAGTACTGCAACTCCAGCGACATCATGGACCTGTTCTGCATCGCCACCGGCCTGCC
TCGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGAATTCAGAACGCACTCCGTACAAAGTGAGA
CCTGTGGCCATCAAGCAACTCTCCGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCAGTTCTCAAGAGCATTCAAAATCAATGAAC
TGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATTAA
GAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGAT
GTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGA
GCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTA
CAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAA
ACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGG
TCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACC
TGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATG
GAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCG
CAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAA
AGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATG
CTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTG
GGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGG
CTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACA
AAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F1 (forward primer)
  • refseq_PDE9A.R9 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKVIFSKYCNSSDIMDLFCIATGLP
RNTTISLLTTDDAMVSIDPTMPANSERTPYKVR
PVAIKQLSEREELIQSVLAQVAEQFSRAFKINE
LKAEVANHLAVLEKRVELEGLKVVEIEKCKSDIK
KMREELAARSSRTNCPCKYSFLDNHKKLTPRRD
VPTYPKYLLSPETIEALRKPTFDVWLWEPNEML
SCLEHMYHDLGLVRDFSINPVTLRRWLFCVHDNY
RNNPFHNFRHCFCVAQMMYSMVWLCSLQEKFSQ
TDILILMTAAICHDLDHPGYNNTYQINARTELA
VRYNDISPLENHHCAVAFQILAEPECNIFSNIPP
DGFKQIRQGMITLILATDMARHAEIMDSFKEKM
ENFDYSNEEHMTLLKMILIKCCDISNEVRPMEV
AEPWVDCLLEEYFMQSDREKSEGLPVAPFMDRDK
VTKATAQIGFIKFVLIPMFETVTKLFPMVEEIM
LQPLWESRDRYEELKRIDDAMKELQKKTDSLTS
GATEKSRERSRDVKNSEGDCA*GKRGAWLQFWTG
WPSCAGSLCREELPWAPGTTRPCFLRTILFTDT
KKKKGIHDAVQNFIFKLSFK*YILIRK

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRR*SSASTATPATSWTCSASPPAC
LGTRPSPC*PPTTPWSPSTPPCPRIQNALRTK*D
LWPSSNSPREKN*SRACWRRLQSSSQEHSKSMN
*KLKLQITWLS*RNAWNWKD*KWWRLRNARVTL
RR*GRSWRPEAAGPTAPVSTVFWITTRS*LLDAM
FPLTPSTCSLQRPSRPCGSRPLTSGFGSPMRC*
AAWSTCTTTSGWSGTSASTLSPSGGGCSASTTT
TETTPSTTSGTASAWPR*CTAWSGSAVSRRSSHK
RIS*S**QRPSATIWTIPATTTRTRSMPAQSWR
SATMTSHRWRTTTAPWPSRSSPSLSATSSPTSH
LMGSSRSDRE*SH*SWPLTWQDMQKLWILSKRKW
RILTTATRST*PC*R*F**NAVISLTRSVQWKS
QSLGWTVY*RNILCRATVRSQKAFLWHRSWTET
K*PRPQPRLGSSSLS*SQCLKQ*PSSSPWLRRSC
CSHFGNPEIATRS*SG*MTP*KSYRRRLTA*RL
GPPRSPEREAEM*KTVKETVPEESGGRGCSSGR
AGRAARDPCAGKSCPGHLAPQDHVF*EPFCSLIQ
KKKKEFMMLYRILFLNCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGNLQQVLQLQRHHGPVLHRHRPA
SEHDHLPADHRRRHGLHRPHHAREFRTHSVQSE
TCGHQATLRERRINPERAGAGCRAVLKSIQNQ*T
ES*SCKSLGCPRETRGIGRTKSGGD*EMQE*H*
EDEGGAGGQKQQDQLPL*VQFFG*PQEVDSSTR
CSHLPQVPALSRDHRGPAEADL*RLALGAQ*DAE
LPGAHVPRPRAGQGLQHQPCHPQEVAVLRPRQL
QKQPLPQLPALLLRGPDDVQHGLALQSPGEVLT
NGYPDPNDSGHLPRSGPSRLQQHVPDQCPHRAGG
PLQ*HLTAGEPPLRRGLPDPRRA*VQHLLQHPT
*WVQADPTGNDHINLGH*HGKTCRNYGFFQREN
GEF*LQQRGAHDPAEDDFDKML*YL*RGPSNGSR
RALGGLFIRGIFYAERP*EVRRPSCGTVHGPRQ
SDQGHSPDWVHQVCPDPNV*NSDQALPHG*GDH
AAATLGIPRSLRGAEADR*RHERVTEED*QLDVW
GHREVQREKQRCEKQ*RRLCLRKAGGVAAVLDG
LAELRGILVQGRAALGTWHHKTMFSKNHFVH*Y
KKKKRNS*CCTEFYF*TVF*IIYSYTE

Sequence NM_001001571

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGA
ATTCAGAACGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCA
GTTCTCAAGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTG
GAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATA
ACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGT
CTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGG
AGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGC
TCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTA
CCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCT
GAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAG
AAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATAT
CTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTT
CCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCA
AGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTT
ACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAG
CGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTT
TTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCT
TATACGGAAA
  • refseq_PDE9A.F1 (forward primer)
  • refseq_PDE9A.R9 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKEHDHLPADHRRRHGLHRPHHARE
FRTHSVQSETCGHQATLRERRINPERAGAGCRA
VLKSIQNQ*TES*SCKSLGCPRETRGIGRTKSG
GD*EMQE*H*EDEGGAGGQKQQDQLPL*VQFFG*
PQEVDSSTRCSHLPQVPALSRDHRGPAEADL*R
LALGAQ*DAELPGAHVPRPRAGQGLQHQPCHPQ
EVAVLRPRQLQKQPLPQLPALLLRGPDDVQHGLA
LQSPGEVLTNGYPDPNDSGHLPRSGPSRLQQHV
PDQCPHRAGGPLQ*HLTAGEPPLRRGLPDPRRA
*VQHLLQHPT*WVQADPTGNDHINLGH*HGKTCR
NYGFFQRENGEF*LQQRGAHDPAEDDFDKML*Y
L*RGPSNGSRRALGGLFIRGIFYAERP*EVRRP
SCGTVHGPRQSDQGHSPDWVHQVCPDPNV*NSDQ
ALPHG*GDHAAATLGIPRSLRGAEADR*RHERV
TEED*QLDVWGHREVQREKQRCEKQ*RRLCLRK
AGGVAAVLDGLAELRGILVQGRAALGTWHHKTMF
SKNHFVH*YKKKKRNS*CCTEFYF*TVF*IIYS
YTE

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRRNTTISLLTTDDAMVSIDPTMPA
NSERTPYKVRPVAIKQLSEREELIQSVLAQVAEQ
FSRAFKINELKAEVANHLAVLEKRVELEGLKVV
EIEKCKSDIKKMREELAARSSRTNCPCKYSFLD
NHKKLTPRRDVPTYPKYLLSPETIEALRKPTFDV
WLWEPNEMLSCLEHMYHDLGLVRDFSINPVTLR
RWLFCVHDNYRNNPFHNFRHCFCVAQMMYSMVW
LCSLQEKFSQTDILILMTAAICHDLDHPGYNNTY
QINARTELAVRYNDISPLENHHCAVAFQILAEP
ECNIFSNIPPDGFKQIRQGMITLILATDMARHA
EIMDSFKEKMENFDYSNEEHMTLLKMILIKCCDI
SNEVRPMEVAEPWVDCLLEEYFMQSDREKSEGL
PVAPFMDRDKVTKATAQIGFIKFVLIPMFETVT
KLFPMVEEIMLQPLWESRDRYEELKRIDDAMKEL
QKKTDSLTSGATEKSRERSRDVKNSEGDCA*GK
RGAWLQFWTGWPSCAGSLCREELPWAPGTTRPC
FLRTILFTDTKKKKGIHDAVQNFIFKLSFK*YIL
IRK

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGTRPSPC*PPTTPWSPSTPPCPR
IQNALRTK*DLWPSSNSPREKN*SRACWRRLQS
SSQEHSKSMN*KLKLQITWLS*RNAWNWKD*KWW
RLRNARVTLRR*GRSWRPEAAGPTAPVSTVFWI
TTRS*LLDAMFPLTPSTCSLQRPSRPCGSRPLT
SGFGSPMRC*AAWSTCTTTSGWSGTSASTLSPSG
GGCSASTTTTETTPSTTSGTASAWPR*CTAWSG
SAVSRRSSHKRIS*S**QRPSATIWTIPATTTR
TRSMPAQSWRSATMTSHRWRTTTAPWPSRSSPSL
SATSSPTSHLMGSSRSDRE*SH*SWPLTWQDMQ
KLWILSKRKWRILTTATRST*PC*R*F**NAVI
SLTRSVQWKSQSLGWTVY*RNILCRATVRSQKAF
LWHRSWTETK*PRPQPRLGSSSLS*SQCLKQ*P
SSSPWLRRSCCSHFGNPEIATRS*SG*MTP*KS
YRRRLTA*RLGPPRSPEREAEM*KTVKETVPEES
GGRGCSSGRAGRAARDPCAGKSCPGHLAPQDHV
F*EPFCSLIQKKKKEFMMLYRILFLNCLLNNIF
LYG

Sequence NM_001001585

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGA
ATTCAGAACGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGCTGGTGTCGAGGACAAGAGAACCACAAGCCGTGGCCAGTCTGCTGA
GAGACCACTGAGGGACAGACGGGTTGTGGGCCTGGAGCAGCCCCGGAGGGAAGGAGCATTTGAAAGTGGACAGGTAGAGCCCAGGCCCAGAGAGCCCCAG
GGCTGCTACCAGGAAGGCCAGCGCATCCCTCCAGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGT
GGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGC
CCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGG
CCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTT
CAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCC
CAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGG
ACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGT
GGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTG
GCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGA
TGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAG
CGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTG
ATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGC
GGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAG
TGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGG
GCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTA
AACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F1 (forward primer)
  • refseq_PDE9A.R9 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKEHDHLPADHRRRHGLHRPHHARE
FRTHSVQSETCGHQATLRWCRGQENHKPWPVC*
ETTEGQTGCGPGAAPEGRSI*KWTGRAQAQRAP
GLLPGRPAHPSRAFKINELKAEVANHLAVLEKRV
ELEGLKVVEIEKCKSDIKKMREELAARSSRTNC
PCKYSFLDNHKKLTPRRDVPTYPKYLLSPETIE
ALRKPTFDVWLWEPNEMLSCLEHMYHDLGLVRDF
SINPVTLRRWLFCVHDNYRNNPFHNFRHCFCVA
QMMYSMVWLCSLQEKFSQTDILILMTAAICHDL
DHPGYNNTYQINARTELAVRYNDISPLENHHCAV
AFQILAEPECNIFSNIPPDGFKQIRQGMITLIL
ATDMARHAEIMDSFKEKMENFDYSNEEHMTLLK
MILIKCCDISNEVRPMEVAEPWVDCLLEEYFMQS
DREKSEGLPVAPFMDRDKVTKATAQIGFIKFVL
IPMFETVTKLFPMVEEIMLQPLWESRDRYEELK
RIDDAMKELQKKTDSLTSGATEKSRERSRDVKNS
EGDCA*GKRGAWLQFWTGWPSCAGSLCREELPW
APGTTRPCFLRTILFTDTKKKKGIHDAVQNFIF
KLSFK*YILIRK

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRRNTTISLLTTDDAMVSIDPTMPA
NSERTPYKVRPVAIKQLSAGVEDKRTTSRGQSAE
RPLRDRRVVGLEQPRREGAFESGQVEPRPREPQ
GCYQEGQRIPPEHSKSMN*KLKLQITWLS*RNA
WNWKD*KWWRLRNARVTLRR*GRSWRPEAAGPTA
PVSTVFWITTRS*LLDAMFPLTPSTCSLQRPSR
PCGSRPLTSGFGSPMRC*AAWSTCTTTSGWSGT
SASTLSPSGGGCSASTTTTETTPSTTSGTASAWP
R*CTAWSGSAVSRRSSHKRIS*S**QRPSATIW
TIPATTTRTRSMPAQSWRSATMTSHRWRTTTAP
WPSRSSPSLSATSSPTSHLMGSSRSDRE*SH*SW
PLTWQDMQKLWILSKRKWRILTTATRST*PC*R
*F**NAVISLTRSVQWKSQSLGWTVY*RNILCR
ATVRSQKAFLWHRSWTETK*PRPQPRLGSSSLS*
SQCLKQ*PSSSPWLRRSCCSHFGNPEIATRS*S
G*MTP*KSYRRRLTA*RLGPPRSPEREAEM*KT
VKETVPEESGGRGCSSGRAGRAARDPCAGKSCPG
HLAPQDHVF*EPFCSLIQKKKKEFMMLYRILFL
NCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGTRPSPC*PPTTPWSPSTPPCPR
IQNALRTK*DLWPSSNSPLVSRTREPQAVASLL
RDH*GTDGLWAWSSPGGKEHLKVDR*SPGPESPR
AATRKASASLQSIQNQ*TES*SCKSLGCPRETR
GIGRTKSGGD*EMQE*H*EDEGGAGGQKQQDQL
PL*VQFFG*PQEVDSSTRCSHLPQVPALSRDHRG
PAEADL*RLALGAQ*DAELPGAHVPRPRAGQGL
QHQPCHPQEVAVLRPRQLQKQPLPQLPALLLRG
PDDVQHGLALQSPGEVLTNGYPDPNDSGHLPRSG
PSRLQQHVPDQCPHRAGGPLQ*HLTAGEPPLRR
GLPDPRRA*VQHLLQHPT*WVQADPTGNDHINL
GH*HGKTCRNYGFFQRENGEF*LQQRGAHDPAED
DFDKML*YL*RGPSNGSRRALGGLFIRGIFYAE
RP*EVRRPSCGTVHGPRQSDQGHSPDWVHQVCP
DPNV*NSDQALPHG*GDHAAATLGIPRSLRGAEA
DR*RHERVTEED*QLDVWGHREVQREKQRCEKQ
*RRLCLRKAGGVAAVLDGLAELRGILVQGRAAL
GTWHHKTMFSKNHFVH*YKKKKRNS*CCTEFYF*
TVF*IIYSYTE

Sequence NM_001001578

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGA
ATTCAGAACGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTG
GCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCA
GAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCT
CTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGAC
CTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCC
GGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGC
GGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTG
GAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGG
GAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGA
GCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTA
GAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTG
GGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGA
TCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGA
AGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGT
GCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGC
TGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F1 (forward primer)
  • refseq_PDE9A.R9 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKEHDHLPADHRRRHGLHRPHHARE
FRTHSVQSETCGHQATLRAFKINELKAEVANHL
AVLEKRVELEGLKVVEIEKCKSDIKKMREELAA
RSSRTNCPCKYSFLDNHKKLTPRRDVPTYPKYLL
SPETIEALRKPTFDVWLWEPNEMLSCLEHMYHD
LGLVRDFSINPVTLRRWLFCVHDNYRNNPFHNF
RHCFCVAQMMYSMVWLCSLQEKFSQTDILILMTA
AICHDLDHPGYNNTYQINARTELAVRYNDISPL
ENHHCAVAFQILAEPECNIFSNIPPDGFKQIRQ
GMITLILATDMARHAEIMDSFKEKMENFDYSNEE
HMTLLKMILIKCCDISNEVRPMEVAEPWVDCLL
EEYFMQSDREKSEGLPVAPFMDRDKVTKATAQI
GFIKFVLIPMFETVTKLFPMVEEIMLQPLWESRD
RYEELKRIDDAMKELQKKTDSLTSGATEKSRER
SRDVKNSEGDCA*GKRGAWLQFWTGWPSCAGSL
CREELPWAPGTTRPCFLRTILFTDTKKKKGIHDA
VQNFIFKLSFK*YILIRK

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRRNTTISLLTTDDAMVSIDPTMPA
NSERTPYKVRPVAIKQLSEHSKSMN*KLKLQITW
LS*RNAWNWKD*KWWRLRNARVTLRR*GRSWRP
EAAGPTAPVSTVFWITTRS*LLDAMFPLTPSTC
SLQRPSRPCGSRPLTSGFGSPMRC*AAWSTCTTT
SGWSGTSASTLSPSGGGCSASTTTTETTPSTTS
GTASAWPR*CTAWSGSAVSRRSSHKRIS*S**Q
RPSATIWTIPATTTRTRSMPAQSWRSATMTSHRW
RTTTAPWPSRSSPSLSATSSPTSHLMGSSRSDR
E*SH*SWPLTWQDMQKLWILSKRKWRILTTATR
ST*PC*R*F**NAVISLTRSVQWKSQSLGWTVY*
RNILCRATVRSQKAFLWHRSWTETK*PRPQPRL
GSSSLS*SQCLKQ*PSSSPWLRRSCCSHFGNPE
IATRS*SG*MTP*KSYRRRLTA*RLGPPRSPERE
AEM*KTVKETVPEESGGRGCSSGRAGRAARDPC
AGKSCPGHLAPQDHVF*EPFCSLIQKKKKEFMM
LYRILFLNCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGTRPSPC*PPTTPWSPSTPPCPR
IQNALRTK*DLWPSSNSPSIQNQ*TES*SCKSL
GCPRETRGIGRTKSGGD*EMQE*H*EDEGGAGGQ
KQQDQLPL*VQFFG*PQEVDSSTRCSHLPQVPA
LSRDHRGPAEADL*RLALGAQ*DAELPGAHVPR
PRAGQGLQHQPCHPQEVAVLRPRQLQKQPLPQLP
ALLLRGPDDVQHGLALQSPGEVLTNGYPDPNDS
GHLPRSGPSRLQQHVPDQCPHRAGGPLQ*HLTA
GEPPLRRGLPDPRRA*VQHLLQHPT*WVQADPTG
NDHINLGH*HGKTCRNYGFFQRENGEF*LQQRG
AHDPAEDDFDKML*YL*RGPSNGSRRALGGLFI
RGIFYAERP*EVRRPSCGTVHGPRQSDQGHSPDW
VHQVCPDPNV*NSDQALPHG*GDHAAATLGIPR
SLRGAEADR*RHERVTEED*QLDVWGHREVQRE
KQRCEKQ*RRLCLRKAGGVAAVLDGLAELRGILV
QGRAALGTWHHKTMFSKNHFVH*YKKKKRNS*C
CTEFYF*TVF*IIYSYTE

Sequence NM_001001582

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGA
ATTCAGAACGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGCTGGTGTCGAGGACAAGAGAACCACAAGCCGTGGCCAGTCTGCTGA
GAGACCACTGAGGGACAGACGGGTTGTGGGCCTGGAGCAGCCCCGGAGGGAAGGAGCATTTGAAAGTGGACAGGTAGAGCCCAGGCCCAGAGAGCCCCAG
GGCTGCTACCAGGAAGGCCAGCGCATCCCTCCAGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCAGTTCTCAAGAGCATTCAAAA
TCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAG
TGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCT
CGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATG
AGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCA
CGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAG
TTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAG
AGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAA
CATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAA
GAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAA
TGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGA
CCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAG
GAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCT
TGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTT
CTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTC
ACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F1 (forward primer)
  • refseq_PDE9A.R9 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKEHDHLPADHRRRHGLHRPHHARE
FRTHSVQSETCGHQATLRWCRGQENHKPWPVC*
ETTEGQTGCGPGAAPEGRSI*KWTGRAQAQRAP
GLLPGRPAHPSRERRINPERAGAGCRAVLKSIQN
Q*TES*SCKSLGCPRETRGIGRTKSGGD*EMQE
*H*EDEGGAGGQKQQDQLPL*VQFFG*PQEVDS
STRCSHLPQVPALSRDHRGPAEADL*RLALGAQ*
DAELPGAHVPRPRAGQGLQHQPCHPQEVAVLRP
RQLQKQPLPQLPALLLRGPDDVQHGLALQSPGE
VLTNGYPDPNDSGHLPRSGPSRLQQHVPDQCPHR
AGGPLQ*HLTAGEPPLRRGLPDPRRA*VQHLLQ
HPT*WVQADPTGNDHINLGH*HGKTCRNYGFFQ
RENGEF*LQQRGAHDPAEDDFDKML*YL*RGPSN
GSRRALGGLFIRGIFYAERP*EVRRPSCGTVHG
PRQSDQGHSPDWVHQVCPDPNV*NSDQALPHG*
GDHAAATLGIPRSLRGAEADR*RHERVTEED*QL
DVWGHREVQREKQRCEKQ*RRLCLRKAGGVAAV
LDGLAELRGILVQGRAALGTWHHKTMFSKNHFV
H*YKKKKRNS*CCTEFYF*TVF*IIYSYTE

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRRNTTISLLTTDDAMVSIDPTMPA
NSERTPYKVRPVAIKQLSAGVEDKRTTSRGQSAE
RPLRDRRVVGLEQPRREGAFESGQVEPRPREPQ
GCYQEGQRIPPEREELIQSVLAQVAEQFSRAFK
INELKAEVANHLAVLEKRVELEGLKVVEIEKCKS
DIKKMREELAARSSRTNCPCKYSFLDNHKKLTP
RRDVPTYPKYLLSPETIEALRKPTFDVWLWEPN
EMLSCLEHMYHDLGLVRDFSINPVTLRRWLFCVH
DNYRNNPFHNFRHCFCVAQMMYSMVWLCSLQEK
FSQTDILILMTAAICHDLDHPGYNNTYQINART
ELAVRYNDISPLENHHCAVAFQILAEPECNIFSN
IPPDGFKQIRQGMITLILATDMARHAEIMDSFK
EKMENFDYSNEEHMTLLKMILIKCCDISNEVRP
MEVAEPWVDCLLEEYFMQSDREKSEGLPVAPFMD
RDKVTKATAQIGFIKFVLIPMFETVTKLFPMVE
EIMLQPLWESRDRYEELKRIDDAMKELQKKTDS
LTSGATEKSRERSRDVKNSEGDCA*GKRGAWLQF
WTGWPSCAGSLCREELPWAPGTTRPCFLRTILF
TDTKKKKGIHDAVQNFIFKLSFK*YILIRK

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGTRPSPC*PPTTPWSPSTPPCPR
IQNALRTK*DLWPSSNSPLVSRTREPQAVASLL
RDH*GTDGLWAWSSPGGKEHLKVDR*SPGPESPR
AATRKASASLQREKN*SRACWRRLQSSSQEHSK
SMN*KLKLQITWLS*RNAWNWKD*KWWRLRNAR
VTLRR*GRSWRPEAAGPTAPVSTVFWITTRS*LL
DAMFPLTPSTCSLQRPSRPCGSRPLTSGFGSPM
RC*AAWSTCTTTSGWSGTSASTLSPSGGGCSAS
TTTTETTPSTTSGTASAWPR*CTAWSGSAVSRRS
SHKRIS*S**QRPSATIWTIPATTTRTRSMPAQ
SWRSATMTSHRWRTTTAPWPSRSSPSLSATSSP
TSHLMGSSRSDRE*SH*SWPLTWQDMQKLWILSK
RKWRILTTATRST*PC*R*F**NAVISLTRSVQ
WKSQSLGWTVY*RNILCRATVRSQKAFLWHRSW
TETK*PRPQPRLGSSSLS*SQCLKQ*PSSSPWLR
RSCCSHFGNPEIATRS*SG*MTP*KSYRRRLTA
*RLGPPRSPEREAEM*KTVKETVPEESGGRGCS
SGRAGRAARDPCAGKSCPGHLAPQDHVF*EPFCS
LIQKKKKEFMMLYRILFLNCLLNNIFLYG

Sequence NM_001001570

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGTAATCTTCAGCAAGTACTGCAACTCCAGCGACATCATGGACCTGTTCTGCATCGCCACCGGCCTGCC
TCGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGAATTCAGAACGGAATGAGCTCATTCTCTAT
ACATCACTCCGTAACTTGTTGTTTTTACCTAGTAAGGAGTCATGGGCGTCCCACCAGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCC
GAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGA
GAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAG
AAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTT
GGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCT
GTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGT
CTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCA
ATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAA
CATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATG
GATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACG
AGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGC
ACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTC
CCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGA
AGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGC
GTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGA
ACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGA
AA
  • refseq_PDE9A.F1 (forward primer)
  • refseq_PDE9A.R9 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKVIFSKYCNSSDIMDLFCIATGLP
RNTTISLLTTDDAMVSIDPTMPANSERNELILY
TSLRNLLFLPSKESWASHQHSVQSETCGHQATL
RAFKINELKAEVANHLAVLEKRVELEGLKVVEIE
KCKSDIKKMREELAARSSRTNCPCKYSFLDNHK
KLTPRRDVPTYPKYLLSPETIEALRKPTFDVWL
WEPNEMLSCLEHMYHDLGLVRDFSINPVTLRRWL
FCVHDNYRNNPFHNFRHCFCVAQMMYSMVWLCS
LQEKFSQTDILILMTAAICHDLDHPGYNNTYQI
NARTELAVRYNDISPLENHHCAVAFQILAEPECN
IFSNIPPDGFKQIRQGMITLILATDMARHAEIM
DSFKEKMENFDYSNEEHMTLLKMILIKCCDISN
EVRPMEVAEPWVDCLLEEYFMQSDREKSEGLPVA
PFMDRDKVTKATAQIGFIKFVLIPMFETVTKLF
PMVEEIMLQPLWESRDRYEELKRIDDAMKELQK
KTDSLTSGATEKSRERSRDVKNSEGDCA*GKRGA
WLQFWTGWPSCAGSLCREELPWAPGTTRPCFLR
TILFTDTKKKKGIHDAVQNFIFKLSFK*YILIR
K

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRR*SSASTATPATSWTCSASPPAC
LGTRPSPC*PPTTPWSPSTPPCPRIQNGMSSFSI
HHSVTCCFYLVRSHGRPTSTPYKVRPVAIKQLS
EHSKSMN*KLKLQITWLS*RNAWNWKD*KWWRL
RNARVTLRR*GRSWRPEAAGPTAPVSTVFWITTR
S*LLDAMFPLTPSTCSLQRPSRPCGSRPLTSGF
GSPMRC*AAWSTCTTTSGWSGTSASTLSPSGGG
CSASTTTTETTPSTTSGTASAWPR*CTAWSGSAV
SRRSSHKRIS*S**QRPSATIWTIPATTTRTRS
MPAQSWRSATMTSHRWRTTTAPWPSRSSPSLSA
TSSPTSHLMGSSRSDRE*SH*SWPLTWQDMQKLW
ILSKRKWRILTTATRST*PC*R*F**NAVISLT
RSVQWKSQSLGWTVY*RNILCRATVRSQKAFLW
HRSWTETK*PRPQPRLGSSSLS*SQCLKQ*PSSS
PWLRRSCCSHFGNPEIATRS*SG*MTP*KSYRR
RLTA*RLGPPRSPEREAEM*KTVKETVPEESGG
RGCSSGRAGRAARDPCAGKSCPGHLAPQDHVF*E
PFCSLIQKKKKEFMMLYRILFLNCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGNLQQVLQLQRHHGPVLHRHRPA
SEHDHLPADHRRRHGLHRPHHAREFRTE*AHSL
YITP*LVVFT**GVMGVPPALRTK*DLWPSSNSP
SIQNQ*TES*SCKSLGCPRETRGIGRTKSGGD*
EMQE*H*EDEGGAGGQKQQDQLPL*VQFFG*PQ
EVDSSTRCSHLPQVPALSRDHRGPAEADL*RLAL
GAQ*DAELPGAHVPRPRAGQGLQHQPCHPQEVA
VLRPRQLQKQPLPQLPALLLRGPDDVQHGLALQ
SPGEVLTNGYPDPNDSGHLPRSGPSRLQQHVPDQ
CPHRAGGPLQ*HLTAGEPPLRRGLPDPRRA*VQ
HLLQHPT*WVQADPTGNDHINLGH*HGKTCRNY
GFFQRENGEF*LQQRGAHDPAEDDFDKML*YL*R
GPSNGSRRALGGLFIRGIFYAERP*EVRRPSCG
TVHGPRQSDQGHSPDWVHQVCPDPNV*NSDQAL
PHG*GDHAAATLGIPRSLRGAEADR*RHERVTEE
D*QLDVWGHREVQREKQRCEKQ*RRLCLRKAGG
VAAVLDGLAELRGILVQGRAALGTWHHKTMFSK
NHFVH*YKKKKRNS*CCTEFYF*TVF*IIYSYTE

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