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Sequence BNIP1.bAug05
CCTGCCGCTGCCCGTAGCCGGCGTCCCCAACATGGCGGCTCCCCAAGACGTCCACGTCCGGATCTGTAACCAAGAGATTGTCAAATTTGACCTGGAGGTGAAGGCGCTTATTCAGGATATCCGTGATTGTTCAGGACCCTTAAGTGCTCTTACTGAACTGAATACTAAAGTAAAAGAGAAATTTCAACAGTTGCGTCACA
GAATACAGCCAGTTCTCTATCAAAGGGCATTTATTTGGACTGCTTCCACATTTTTTTTTAAGCTAACTTATTCCCTGACAGACTTTTCTTCAACTCAGCA
TGACTTCAACTCTCCAACTACACCTGTTACCTTCAGTGACCTGGAGCAGTTGGCTAAAGAGCAAGACAAAGAATCAGAGAAACAACTTCTACTCCAGGAA
GTGGAGAATCACAAAAAGCAGATGCTCAGGAAAACCACCAAAGAGAGCCTGGCCCAGACATCCAGTACCATCACTGAGAGCCTCATGGGGATCAGCAGGA
TGATGGCCCAGCAGGTCCAGCAGAGCGAGGAGGCCATGCAGTCTCTAGTCACTTCTTCACGAACGATCCTGGATGCAAATGAAGAATTTAAGTCCATGTC
GGGCACCATCCAGCTGGGCCGGAAGCTTATCACAAAATACAATCGCCGGGAGCTGACGGACAAGCTTCTCATCTTCCTTGCGCTAGCCCTGTTTCTTGCT
ACGGTCCTCTATATTGTGAAAAAGCGGCTCTTTCCATTTTTGTGAGATCCCAAAGGTGCCAGTTCTGGCCCTTTCAGCTCCTGTTTCAGGATCTGTCCTG
GTTCCTGAGCTCTAGGCTGCTAAGCTGAGCCACACACCCCTCCGTTTGCACCAGTTGCCTGCAGGTTGGATGGAACACAGTGCCCCACTTTTCTGCAAGT
AGCTGGCTTGTAAAGGGTGAACAGAGCCATGGGAGGAAGGTCTGGCATTGGGATGCCGCCCTGGGGACATACGAACCGCCTCCTTCCACCATTGTGCACT
ATGGGAGGCCGCTGCTGCGTGGAGCACTTAAAGTCCAGCCTCCAGGACCGGATGCCCCTCCTGTCTCCCGCTCCCATCGTGCCCTTAAATGCCAGATCTG
GTGGAGGGAAGAGAGAAGAGGTAGGAAGAAAGGTGATGAAAACTCCTGGTCATTCCTTGAATAAACTTGATTTTATTTAATAGAATAAAATGTATTTGAT
TTTGTAAGATA
- BNIP1-7 (forward primer)
- BNIP1-8 (reverse primer)
- Single exon skipping
Frame 0
PAAARSRRPQHGGSPRRPRPDL*PRDCQI*PGGEGAYSGYP*LFRTLKCSY*TEY*SKREISTVASQ
NTASSLSKGIYLDCFHIFF*ANLFPDRLFFNSA
*LQLSNYTCYLQ*PGAVG*RARQRIRETTSTPG
SGESQKADAQENHQREPGPDIQYHH*EPHGDQQD
DGPAGPAERGGHAVSSHFFTNDPGCK*RI*VHV
GHHPAGPEAYHKIQSPGADGQASHLPCASPVSC
YGPLYCEKAALSIFVRSQRCQFWPFQLLFQDLSW
FLSSRLLS*ATHPSVCTSCLQVGWNTVPHFSAS
SWLVKGEQSHGRKVWHWDAALGTYEPPPSTIVH
YGRPLLRGALKVQPPGPDAPPVSRSHRALKCQIW
WREERRGRKKGDENSWSFLE*T*FYLIE*NVFD
FVR
Frame 1
LPLPVAGVPNMAAPQDVHVRICNQEIVKFDLEVKALIQDIRDCSGPLSALTELNTKVKEKFQQLRH
RIQPVLYQRAFIWTASTFFFKLTYSLTDFSSTQH
DFNSPTTPVTFSDLEQLAKEQDKESEKQLLLQE
VENHKKQMLRKTTKESLAQTSSTITESLMGISR
MMAQQVQQSEEAMQSLVTSSRTILDANEEFKSMS
GTIQLGRKLITKYNRRELTDKLLIFLALALFLA
TVLYIVKKRLFPFL*DPKGASSGPFSSCFRICP
GS*ALGC*AEPHTPPFAPVACRLDGTQCPTFLQV
AGL*RVNRAMGGRSGIGMPPWGHTNRLLPPLCT
MGGRCCVEHLKSSLQDRMPLLSPAPIVPLNARS
GGGKREEVGRKVMKTPGHSLNKLDFI**NKMYLI
L*D
Frame 2
CRCP*PASPTWRLPKTSTSGSVTKRLSNLTWR*RRLFRISVIVQDP*VLLLN*ILK*KRNFNSCVT
EYSQFSIKGHLFGLLPHFFLS*LIP*QTFLQLS
MTSTLQLHLLPSVTWSSWLKSKTKNQRNNFYSRK
WRITKSRCSGKPPKRAWPRHPVPSLRASWGSAG
*WPSRSSRARRPCSL*SLLHERSWMQMKNLSPC
RAPSSWAGSLSQNTIAGS*RTSFSSSLR*PCFLL
RSSIL*KSGSFHFCEIPKVPVLALSAPVSGSVL
VPEL*AAKLSHTPLRLHQLPAGWMEHSAPLFCK
*LACKG*TEPWEEGLALGCRPGDIRTASFHHCAL
WEAAAAWST*SPASRTGCPSCLPLPSCP*MPDL
VEGREKR*EER**KLLVIP*INLILFNRIKCI*
FCKI
Sequence BNIP1.aAug05
CCTGCCGCTGCCCGTAGCCGGCGTCCCCAACATGGCGGCTCCCCAAGACGTCCACGTCCGGATCTGTAACCAAGAGATTGTCAAATTTGACCTGGAGGTGAAGGCGCTTATTCAGGATATCCGTGATTGTTCAGGACCCTTAAGTGCTCTTACTGAACTGAATACTAAAGTAAAAGAGAAATTTCAACAGTTGCGTCACA
GAATACAGCCAGTTCTCTATCAAAGGGCATTTATTTGGACTGCTTCCACATTTTTTTTTAAGCTAACTTATTCCCTGACAGACTTTTCTTCAACTCAGCA
TGACTTCAACTCTCCAACTACACCTGTTACCTTCAGTGACCTGGAGCAGTTGGCTAAAGAGCAAGACAAAGAATCAGAGAAACAACTTCTACTCCAGGAA
GTGGAGAATCACAAAAAGCAGATGCTCAGCAATCAGGCCTCATGGAGGAAAGCTAATCTCACCTGCAAAATTGCAATCGACAATCTAGAGAAAGCAGAAC
TTCTTCAGGGAGGAGATCTCTTAAGGCAAAGGAAAACCACCAAAGAGAGCCTGGCCCAGACATCCAGTACCATCACTGAGAGCCTCATGGGGATCAGCAG
GATGATGGCCCAGCAGGTCCAGCAGAGCGAGGAGGCCATGCAGTCTCTAGTCACTTCTTCACGAACGATCCTGGATGCAAATGAAGAATTTAAGTCCATG
TCGGGCACCATCCAGCTGGGCCGGAAGCTTATCACAAAATACAATCGCCGGGAGCTGACGGACAAGCTTCTCATCTTCCTTGCGCTAGCCCTGTTTCTTG
CTACGGTCCTCTATATTGTGAAAAAGCGGCTCTTTCCATTTTTGTGAGATCCCAAAGGTGCCAGTTCTGGCCCTTTCAGCTCCTGTTTCAGGATCTGTCC
TGGTTCCTGAGCTCTAGGCTGCTAAGCTGAGCCACACACCCCTCCGTTTGCACCAGTTGCCTGCAGGTTGGATGGAACACAGTGCCCCACTTTTCTGCAA
GTAGCTGGCTTGTAAAGGGTGAACAGAGCCATGGGAGGAAGGTCTGGCATTGGGATGCCGCCCTGGGGACATACGAACCGCCTCCTTCCACCATTGTGCA
CTATGGGAGGCCGCTGCTGCGTGGAGCACTTAAAGTCCAGCCTCCAGGACCGGATGCCCCTCCTGTCTCCCGCTCCCATCGTGCCCTTAAATGCCAGATC
TGGTGGAGGGAAGAGAGAAGAGGTAGGAAGAAAGGTGATGAAAACTCCTGGTCATTCCTTGAATAAACTTGATTTTATTTAATAGAATAAAATGTATTTG
ATTTTGTAAGATA
- BNIP1-7 (forward primer)
- BNIP1-8 (reverse primer)
- Single exon skipping
Frame 0
PAAARSRRPQHGGSPRRPRPDL*PRDCQI*PGGEGAYSGYP*LFRTLKCSY*TEY*SKREISTVASQ
NTASSLSKGIYLDCFHIFF*ANLFPDRLFFNSA
*LQLSNYTCYLQ*PGAVG*RARQRIRETTSTPG
SGESQKADAQQSGLMEES*SHLQNCNRQSRESRT
SSGRRSLKAKENHQREPGPDIQYHH*EPHGDQQ
DDGPAGPAERGGHAVSSHFFTNDPGCK*RI*VH
VGHHPAGPEAYHKIQSPGADGQASHLPCASPVSC
YGPLYCEKAALSIFVRSQRCQFWPFQLLFQDLS
WFLSSRLLS*ATHPSVCTSCLQVGWNTVPHFSA
SSWLVKGEQSHGRKVWHWDAALGTYEPPPSTIVH
YGRPLLRGALKVQPPGPDAPPVSRSHRALKCQI
WWREERRGRKKGDENSWSFLE*T*FYLIE*NVF
DFVR
Frame 1
LPLPVAGVPNMAAPQDVHVRICNQEIVKFDLEVKALIQDIRDCSGPLSALTELNTKVKEKFQQLRH
RIQPVLYQRAFIWTASTFFFKLTYSLTDFSSTQH
DFNSPTTPVTFSDLEQLAKEQDKESEKQLLLQE
VENHKKQMLSNQASWRKANLTCKIAIDNLEKAE
LLQGGDLLRQRKTTKESLAQTSSTITESLMGISR
MMAQQVQQSEEAMQSLVTSSRTILDANEEFKSM
SGTIQLGRKLITKYNRRELTDKLLIFLALALFL
ATVLYIVKKRLFPFL*DPKGASSGPFSSCFRICP
GS*ALGC*AEPHTPPFAPVACRLDGTQCPTFLQ
VAGL*RVNRAMGGRSGIGMPPWGHTNRLLPPLC
TMGGRCCVEHLKSSLQDRMPLLSPAPIVPLNARS
GGGKREEVGRKVMKTPGHSLNKLDFI**NKMYL
IL*D
Frame 2
CRCP*PASPTWRLPKTSTSGSVTKRLSNLTWR*RRLFRISVIVQDP*VLLLN*ILK*KRNFNSCVT
EYSQFSIKGHLFGLLPHFFLS*LIP*QTFLQLS
MTSTLQLHLLPSVTWSSWLKSKTKNQRNNFYSRK
WRITKSRCSAIRPHGGKLISPAKLQSTI*RKQN
FFREEIS*GKGKPPKRAWPRHPVPSLRASWGSA
G*WPSRSSRARRPCSL*SLLHERSWMQMKNLSPC
RAPSSWAGSLSQNTIAGS*RTSFSSSLR*PCFL
LRSSIL*KSGSFHFCEIPKVPVLALSAPVSGSV
LVPEL*AAKLSHTPLRLHQLPAGWMEHSAPLFCK
*LACKG*TEPWEEGLALGCRPGDIRTASFHHCA
LWEAAAAWST*SPASRTGCPSCLPLPSCP*MPD
LVEGREKR*EER**KLLVIP*INLILFNRIKCI*
FCKI
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