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1. Initial RefSeq screen / epithelium and mesenchyme (fetal colon) 
Transcript map : ABCB4 epithelium
Exon
Length: 1274
Transcript: NM_000443
Exon
Length: 340
Transcript: NM_000443
Exon
Length: 58
Transcript: NM_000443
Exon
Length: 140
Transcript: NM_000443
Exon
Length: 26
Transcript: NM_000443
Exon
Length: 86
Transcript: NM_000443
Exon
Length: 55
Transcript: NM_000443
Exon
Length: 151
Transcript: NM_000443
Exon
Length: 58
Transcript: NM_000443
Exon
Length: 192
Transcript: NM_000443
Exon
Length: 172
Transcript: NM_000443
Exon
Length: 125
Transcript: NM_000443
Exon
Length: 172
Transcript: NM_000443
Exon
Length: 114
Transcript: NM_000443
Exon
Length: 111
Transcript: NM_000443
Exon
Length: 126
Transcript: NM_000443
Exon
Length: 204
Transcript: NM_000443
Exon
Length: 171
Transcript: NM_000443
Exon
Length: 162
Transcript: NM_000443
Exon
Length: 171
Transcript: NM_000443
Exon
Length: 147
Transcript: NM_000443
Exon
Length: 105
Transcript: NM_000443
Exon
Length: 78
Transcript: NM_000443
Exon
Length: 84
Transcript: NM_000443
Exon
Length: 204
Transcript: NM_000443
Exon
Length: 101
Transcript: NM_000443
Exon
Length: 141
Transcript: NM_000443
Exon
Length: 157
Transcript: NM_000443
Exon
Length: 198
Transcript: NM_000443
Exon
Length: 207
Transcript: NM_000443
Exon
Length: 147
Transcript: NM_000443
Exon
Length: 258
Transcript: NM_000443
Intron
Length: 1611
Transcript: NM_000443
Intron
Length: 156
Transcript: NM_000443
Intron
Length: 718
Transcript: NM_000443
Intron
Length: 476
Transcript: NM_000443
Intron
Length: 162
Transcript: NM_000443
Intron
Length: 2710
Transcript: NM_000443
Intron
Length: 9712
Transcript: NM_000443
Intron
Length: 8165
Transcript: NM_000443
Intron
Length: 1399
Transcript: NM_000443
Intron
Length: 1149
Transcript: NM_000443
Intron
Length: 1530
Transcript: NM_000443
Intron
Length: 2762
Transcript: NM_000443
Intron
Length: 2058
Transcript: NM_000443
Intron
Length: 1088
Transcript: NM_000443
Intron
Length: 218
Transcript: NM_000443
Intron
Length: 2916
Transcript: NM_000443
Intron
Length: 361
Transcript: NM_000443
Intron
Length: 8101
Transcript: NM_000443
Intron
Length: 4483
Transcript: NM_000443
Intron
Length: 2697
Transcript: NM_000443
Intron
Length: 1680
Transcript: NM_000443
Intron
Length: 2045
Transcript: NM_000443
Intron
Length: 1377
Transcript: NM_000443
Intron
Length: 1021
Transcript: NM_000443
Intron
Length: 3594
Transcript: NM_000443
Intron
Length: 1583
Transcript: NM_000443
Intron
Length: 2500
Transcript: NM_000443
Intron
Length: 1001
Transcript: NM_000443
Intron
Length: 1542
Transcript: NM_000443
Intron
Length: 3006
Transcript: NM_000443
Intron
Length: 832
Transcript: NM_000443
Reaction
Expected peak: 106
56.8M
refseq_ABCB4.F1
refseq_ABCB4.R1
Reaction
Expected peak: 320
19M
refseq_ABCB4.F3
refseq_ABCB4.R3
Exon
Length: 1274
Transcript: NM_018849
Exon
Length: 340
Transcript: NM_018849
Exon
Length: 58
Transcript: NM_018849
Exon
Length: 140
Transcript: NM_018849
Exon
Length: 26
Transcript: NM_018849
Exon
Length: 86
Transcript: NM_018849
Exon
Length: 55
Transcript: NM_018849
Exon
Length: 151
Transcript: NM_018849
Exon
Length: 58
Transcript: NM_018849
Exon
Length: 192
Transcript: NM_018849
Exon
Length: 172
Transcript: NM_018849
Exon
Length: 125
Transcript: NM_018849
Exon
Length: 172
Transcript: NM_018849
Exon
Length: 114
Transcript: NM_018849
Exon
Length: 111
Transcript: NM_018849
Exon
Length: 126
Transcript: NM_018849
Exon
Length: 204
Transcript: NM_018849
Exon
Length: 171
Transcript: NM_018849
Exon
Length: 162
Transcript: NM_018849
Exon
Length: 171
Transcript: NM_018849
Exon
Length: 147
Transcript: NM_018849
Exon
Length: 105
Transcript: NM_018849
Exon
Length: 78
Transcript: NM_018849
Exon
Length: 84
Transcript: NM_018849
Exon
Length: 204
Transcript: NM_018849
Exon
Length: 101
Transcript: NM_018849
Exon
Length: 141
Transcript: NM_018849
Exon
Length: 157
Transcript: NM_018849
Exon
Length: 198
Transcript: NM_018849
Exon
Length: 228
Transcript: NM_018849
Exon
Length: 147
Transcript: NM_018849
Exon
Length: 258
Transcript: NM_018849
Intron
Length: 1611
Transcript: NM_018849
Intron
Length: 156
Transcript: NM_018849
Intron
Length: 718
Transcript: NM_018849
Intron
Length: 476
Transcript: NM_018849
Intron
Length: 162
Transcript: NM_018849
Intron
Length: 2710
Transcript: NM_018849
Intron
Length: 9712
Transcript: NM_018849
Intron
Length: 8165
Transcript: NM_018849
Intron
Length: 1399
Transcript: NM_018849
Intron
Length: 1149
Transcript: NM_018849
Intron
Length: 1530
Transcript: NM_018849
Intron
Length: 2762
Transcript: NM_018849
Intron
Length: 2058
Transcript: NM_018849
Intron
Length: 1088
Transcript: NM_018849
Intron
Length: 218
Transcript: NM_018849
Intron
Length: 2916
Transcript: NM_018849
Intron
Length: 361
Transcript: NM_018849
Intron
Length: 8101
Transcript: NM_018849
Intron
Length: 4483
Transcript: NM_018849
Intron
Length: 2697
Transcript: NM_018849
Intron
Length: 1680
Transcript: NM_018849
Intron
Length: 2045
Transcript: NM_018849
Intron
Length: 1377
Transcript: NM_018849
Intron
Length: 1021
Transcript: NM_018849
Intron
Length: 3594
Transcript: NM_018849
Intron
Length: 1583
Transcript: NM_018849
Intron
Length: 2500
Transcript: NM_018849
Intron
Length: 1001
Transcript: NM_018849
Intron
Length: 1521
Transcript: NM_018849
Intron
Length: 3006
Transcript: NM_018849
Intron
Length: 832
Transcript: NM_018849
Reaction
Expected peak: 127
8M
refseq_ABCB4.F1
refseq_ABCB4.R1
Reaction
Expected peak: 320
19M
refseq_ABCB4.F3
refseq_ABCB4.R3
Exon
Length: 1274
Transcript: NM_018850
Exon
Length: 340
Transcript: NM_018850
Exon
Length: 58
Transcript: NM_018850
Exon
Length: 140
Transcript: NM_018850
Exon
Length: 26
Transcript: NM_018850
Exon
Length: 86
Transcript: NM_018850
Exon
Length: 55
Transcript: NM_018850
Exon
Length: 151
Transcript: NM_018850
Exon
Length: 58
Transcript: NM_018850
Exon
Length: 192
Transcript: NM_018850
Exon
Length: 172
Transcript: NM_018850
Exon
Length: 125
Transcript: NM_018850
Exon
Length: 172
Transcript: NM_018850
Exon
Length: 114
Transcript: NM_018850
Exon
Length: 111
Transcript: NM_018850
Exon
Length: 126
Transcript: NM_018850
Exon
Length: 204
Transcript: NM_018850
Exon
Length: 171
Transcript: NM_018850
Exon
Length: 162
Transcript: NM_018850
Exon
Length: 171
Transcript: NM_018850
Exon
Length: 147
Transcript: NM_018850
Exon
Length: 105
Transcript: NM_018850
Exon
Length: 78
Transcript: NM_018850
Exon
Length: 84
Transcript: NM_018850
Exon
Length: 204
Transcript: NM_018850
Exon
Length: 101
Transcript: NM_018850
Exon
Length: 157
Transcript: NM_018850
Exon
Length: 198
Transcript: NM_018850
Exon
Length: 207
Transcript: NM_018850
Exon
Length: 147
Transcript: NM_018850
Exon
Length: 258
Transcript: NM_018850
Intron
Length: 1611
Transcript: NM_018850
Intron
Length: 156
Transcript: NM_018850
Intron
Length: 718
Transcript: NM_018850
Intron
Length: 476
Transcript: NM_018850
Intron
Length: 162
Transcript: NM_018850
Intron
Length: 2710
Transcript: NM_018850
Intron
Length: 9712
Transcript: NM_018850
Intron
Length: 8165
Transcript: NM_018850
Intron
Length: 1399
Transcript: NM_018850
Intron
Length: 1149
Transcript: NM_018850
Intron
Length: 1530
Transcript: NM_018850
Intron
Length: 2762
Transcript: NM_018850
Intron
Length: 2058
Transcript: NM_018850
Intron
Length: 1088
Transcript: NM_018850
Intron
Length: 218
Transcript: NM_018850
Intron
Length: 2916
Transcript: NM_018850
Intron
Length: 361
Transcript: NM_018850
Intron
Length: 8101
Transcript: NM_018850
Intron
Length: 4483
Transcript: NM_018850
Intron
Length: 2697
Transcript: NM_018850
Intron
Length: 1680
Transcript: NM_018850
Intron
Length: 2045
Transcript: NM_018850
Intron
Length: 1377
Transcript: NM_018850
Intron
Length: 1021
Transcript: NM_018850
Intron
Length: 3594
Transcript: NM_018850
Intron
Length: 4224
Transcript: NM_018850
Intron
Length: 1001
Transcript: NM_018850
Intron
Length: 1542
Transcript: NM_018850
Intron
Length: 3006
Transcript: NM_018850
Intron
Length: 832
Transcript: NM_018850
Reaction
Expected peak: 106
56.8M
refseq_ABCB4.F1
refseq_ABCB4.R1
Reaction
Expected peak: 179
20.3M
refseq_ABCB4.F3
refseq_ABCB4.R3
refseq_ABCB4.F1
CTGGAGCGGTTCTACGACC
refseq_ABCB4.R1
GAGACACGATTCCGAGTTGAG
refseq_ABCB4.F3
AGGACAGTTGTGTCTTTGACCC
refseq_ABCB4.R3
AGCATGAATAAGTGGGCTGC

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