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Supplementary data
Tumor microenvironment associated modifications of alternative splicing
Assay reliability
QPsi proof of principle
endpoint PCR
Transcript map
: ADD3 Normal FF \n=1
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019254 B11) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019254 B9) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019254 B7) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019254 B5) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019254 B3) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019254 B1) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019255 B11) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019255 B9) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019255 B7) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019255 B5) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019255 B3) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019255 B1) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019256 B11) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019256 B9) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019256 B7) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019256 B5) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019256 B3) : Curve | Curve set
- refseq_ADD3.F2 / refseq_ADD3.R2 (CM019256 B1) : Curve | Curve set
Exon
Length: 347
Transcript: NM_001121
Length: 347
Transcript: NM_001121
Exon
Length: 224
Transcript: NM_001121
Length: 224
Transcript: NM_001121
Exon
Length: 139
Transcript: NM_001121
Length: 139
Transcript: NM_001121
Exon
Length: 152
Transcript: NM_001121
Length: 152
Transcript: NM_001121
Exon
Length: 81
Transcript: NM_001121
Length: 81
Transcript: NM_001121
Exon
Length: 150
Transcript: NM_001121
Length: 150
Transcript: NM_001121
Exon
Length: 144
Transcript: NM_001121
Length: 144
Transcript: NM_001121
Exon
Length: 99
Transcript: NM_001121
Length: 99
Transcript: NM_001121
Exon
Length: 183
Transcript: NM_001121
Length: 183
Transcript: NM_001121
Exon
Length: 258
Transcript: NM_001121
Length: 258
Transcript: NM_001121
Exon
Length: 120
Transcript: NM_001121
Length: 120
Transcript: NM_001121
Exon
Length: 87
Transcript: NM_001121
Length: 87
Transcript: NM_001121
Exon
Length: 124
Transcript: NM_001121
Length: 124
Transcript: NM_001121
Exon
Length: 2240
Transcript: NM_001121
Length: 2240
Transcript: NM_001121
Intron
Length: 92325
Transcript: NM_001121
Length: 92325
Transcript: NM_001121
Intron
Length: 11928
Transcript: NM_001121
Length: 11928
Transcript: NM_001121
Intron
Length: 3343
Transcript: NM_001121
Length: 3343
Transcript: NM_001121
Intron
Length: 931
Transcript: NM_001121
Length: 931
Transcript: NM_001121
Intron
Length: 1164
Transcript: NM_001121
Length: 1164
Transcript: NM_001121
Intron
Length: 474
Transcript: NM_001121
Length: 474
Transcript: NM_001121
Intron
Length: 111
Transcript: NM_001121
Length: 111
Transcript: NM_001121
Intron
Length: 2545
Transcript: NM_001121
Length: 2545
Transcript: NM_001121
Intron
Length: 1724
Transcript: NM_001121
Length: 1724
Transcript: NM_001121
Intron
Length: 1551
Transcript: NM_001121
Length: 1551
Transcript: NM_001121
Intron
Length: 471
Transcript: NM_001121
Length: 471
Transcript: NM_001121
Intron
Length: 3859
Transcript: NM_001121
Length: 3859
Transcript: NM_001121
Intron
Length: 2839
Transcript: NM_001121
Length: 2839
Transcript: NM_001121
Reaction
Expected peak: 284
0M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
0M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
0M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
0M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
40.8M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
40.8M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
23.4M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
23.4M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
24.4M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
24.4M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
10.3M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
10.3M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
110M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
110M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
15.9M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
15.9M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
147.2M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
147.2M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
151.3M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
151.3M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
117.8M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
117.8M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
126.9M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
126.9M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
122.8M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
122.8M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
14.3M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
14.3M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
220.7M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
220.7M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
135M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
135M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
135.39999M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
135.39999M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
164.3M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
164.3M
refseq_ADD3.F2
refseq_ADD3.R2
Exon
Length: 347
Transcript: NM_016824
Length: 347
Transcript: NM_016824
Exon
Length: 224
Transcript: NM_016824
Length: 224
Transcript: NM_016824
Exon
Length: 139
Transcript: NM_016824
Length: 139
Transcript: NM_016824
Exon
Length: 152
Transcript: NM_016824
Length: 152
Transcript: NM_016824
Exon
Length: 81
Transcript: NM_016824
Length: 81
Transcript: NM_016824
Exon
Length: 150
Transcript: NM_016824
Length: 150
Transcript: NM_016824
Exon
Length: 144
Transcript: NM_016824
Length: 144
Transcript: NM_016824
Exon
Length: 99
Transcript: NM_016824
Length: 99
Transcript: NM_016824
Exon
Length: 183
Transcript: NM_016824
Length: 183
Transcript: NM_016824
Exon
Length: 258
Transcript: NM_016824
Length: 258
Transcript: NM_016824
Exon
Length: 120
Transcript: NM_016824
Length: 120
Transcript: NM_016824
Exon
Length: 87
Transcript: NM_016824
Length: 87
Transcript: NM_016824
Exon
Length: 124
Transcript: NM_016824
Length: 124
Transcript: NM_016824
Exon
Length: 96
Transcript: NM_016824
Length: 96
Transcript: NM_016824
Exon
Length: 2240
Transcript: NM_016824
Length: 2240
Transcript: NM_016824
Intron
Length: 92325
Transcript: NM_016824
Length: 92325
Transcript: NM_016824
Intron
Length: 11928
Transcript: NM_016824
Length: 11928
Transcript: NM_016824
Intron
Length: 3343
Transcript: NM_016824
Length: 3343
Transcript: NM_016824
Intron
Length: 931
Transcript: NM_016824
Length: 931
Transcript: NM_016824
Intron
Length: 1164
Transcript: NM_016824
Length: 1164
Transcript: NM_016824
Intron
Length: 474
Transcript: NM_016824
Length: 474
Transcript: NM_016824
Intron
Length: 111
Transcript: NM_016824
Length: 111
Transcript: NM_016824
Intron
Length: 2545
Transcript: NM_016824
Length: 2545
Transcript: NM_016824
Intron
Length: 1724
Transcript: NM_016824
Length: 1724
Transcript: NM_016824
Intron
Length: 1551
Transcript: NM_016824
Length: 1551
Transcript: NM_016824
Intron
Length: 471
Transcript: NM_016824
Length: 471
Transcript: NM_016824
Intron
Length: 3859
Transcript: NM_016824
Length: 3859
Transcript: NM_016824
Intron
Length: 1818
Transcript: NM_016824
Length: 1818
Transcript: NM_016824
Intron
Length: 925
Transcript: NM_016824
Length: 925
Transcript: NM_016824
Reaction
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
49.7M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
49.7M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
61.2M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
61.2M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
58.8M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
58.8M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
98.5M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
98.5M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
6.1M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
6.1M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
65.5M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
65.5M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
7.3M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
7.3M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
73M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
73M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
1.1M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
1.1M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 380
0M
refseq_ADD3.F2
refseq_ADD3.R2
Exon
Length: 184
Transcript: NM_019903
Length: 184
Transcript: NM_019903
Exon
Length: 224
Transcript: NM_019903
Length: 224
Transcript: NM_019903
Exon
Length: 139
Transcript: NM_019903
Length: 139
Transcript: NM_019903
Exon
Length: 152
Transcript: NM_019903
Length: 152
Transcript: NM_019903
Exon
Length: 81
Transcript: NM_019903
Length: 81
Transcript: NM_019903
Exon
Length: 150
Transcript: NM_019903
Length: 150
Transcript: NM_019903
Exon
Length: 144
Transcript: NM_019903
Length: 144
Transcript: NM_019903
Exon
Length: 99
Transcript: NM_019903
Length: 99
Transcript: NM_019903
Exon
Length: 183
Transcript: NM_019903
Length: 183
Transcript: NM_019903
Exon
Length: 258
Transcript: NM_019903
Length: 258
Transcript: NM_019903
Exon
Length: 120
Transcript: NM_019903
Length: 120
Transcript: NM_019903
Exon
Length: 87
Transcript: NM_019903
Length: 87
Transcript: NM_019903
Exon
Length: 124
Transcript: NM_019903
Length: 124
Transcript: NM_019903
Exon
Length: 2240
Transcript: NM_019903
Length: 2240
Transcript: NM_019903
Intron
Length: 94473
Transcript: NM_019903
Length: 94473
Transcript: NM_019903
Intron
Length: 11928
Transcript: NM_019903
Length: 11928
Transcript: NM_019903
Intron
Length: 3343
Transcript: NM_019903
Length: 3343
Transcript: NM_019903
Intron
Length: 931
Transcript: NM_019903
Length: 931
Transcript: NM_019903
Intron
Length: 1164
Transcript: NM_019903
Length: 1164
Transcript: NM_019903
Intron
Length: 474
Transcript: NM_019903
Length: 474
Transcript: NM_019903
Intron
Length: 111
Transcript: NM_019903
Length: 111
Transcript: NM_019903
Intron
Length: 2545
Transcript: NM_019903
Length: 2545
Transcript: NM_019903
Intron
Length: 1724
Transcript: NM_019903
Length: 1724
Transcript: NM_019903
Intron
Length: 1551
Transcript: NM_019903
Length: 1551
Transcript: NM_019903
Intron
Length: 471
Transcript: NM_019903
Length: 471
Transcript: NM_019903
Intron
Length: 3859
Transcript: NM_019903
Length: 3859
Transcript: NM_019903
Intron
Length: 2839
Transcript: NM_019903
Length: 2839
Transcript: NM_019903
Reaction
Expected peak: 284
0M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
0M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
0M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
0M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
40.8M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
40.8M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
23.4M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
23.4M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
24.4M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
24.4M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
10.3M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
10.3M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
110M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
110M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
15.9M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
15.9M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
147.2M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
147.2M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
151.3M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
151.3M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
117.8M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
117.8M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
126.9M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
126.9M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
122.8M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
122.8M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
14.3M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
14.3M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
220.7M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
220.7M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
135M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
135M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
135.39999M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
135.39999M
refseq_ADD3.F2
refseq_ADD3.R2
Reaction
Expected peak: 284
164.3M
refseq_ADD3.F2
refseq_ADD3.R2
Expected peak: 284
164.3M
refseq_ADD3.F2
refseq_ADD3.R2
refseq_ADD3.F2
CAGGACCACAATCTCAGTTGC
CAGGACCACAATCTCAGTTGC
refseq_ADD3.R2
TCGCTTAGCAAGCTCATCTTC
TCGCTTAGCAAGCTCATCTTC