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LCM Validation sample set, stromal signature 
QPCR Primer Pair List : primer pair list
Gene Forward Reverse
VIEW AFF2 AFF2_G_1_f AFF2_G_r
VIEW BCAS2 BCAS2_G_f BCAS2_G_r
VIEW BUD13 BUD13_G_f BUD13_G_1_r
VIEW C19orf29 C19orf29.G1.F1 C19orf29.G1.R1
VIEW CASC3 CASC3_G_f CASC3_G_r
VIEW CCAR1 CCAR1_G_1_f CCAR1_G_r
VIEW CCDC94 CCDC94.G1.F1 CCDC94.G1.R1
VIEW CDC5L CDC5L for1 CDC5L rev1
VIEW CELF6 BRUNOL6_G_3_f BRUNOL6_G_3_r
VIEW CIR1 CIR_G_1_f CIR_G_r
VIEW CLNS1A CLNS1A_G_1_f CLNS1A_G_r
VIEW CPSF7 FLJ12529_G_1_f FLJ12529_G_1_r
VIEW CWC22 CWC22_G_1_f CWC22_G_r
VIEW CWC27 SDCCAG10_G_f SDCCAG10_G_1_r
VIEW DDX17 DDX17_G_1_f DDX17_G_r
VIEW DDX39 DDX39_G_1_f DDX39_G_r
VIEW DDX3X DDX3X_G_f DDX3X_G_r
VIEW DDX41 DDX41_G_f DDX41_G_r
VIEW DDX46 DDX46_G_f DDX46_G_r
VIEW DHX16 DHX16_G_f DHX16_G_r
QPCR Relative Expression Quantification : Summary

Expression Table

ID Name
1 OVC 517 S
2 OVC 552 S
3 OVC 563 S
4 OVC 78 S
5 OVN 250 S
6 OVN 308 S
7 OVN 458 S
8 OVN 578 S
Primer Pair S1 S2 S3 S4 S5 S6 S7 S8
A2BP1_G_f A2BP1_G_r 1.225 1.000
AFF2_G_1_f AFF2_G_r 2.347 1.108 3.816 6.693 1.394 0.606
BCAS2_G_f BCAS2_G_r 6.028 0.649 4.160 0.544 1.456
BRUNOL6_G_3_f BRUNOL6_G_3_r 0.026 0.274 3.175 0.150 0.402
BUD13_G_f BUD13_G_1_r 1.119 0.529 0.129 0.639 1.421 1.378 0.371 0.829
C19orf29.G1.F1 C19orf29.G1.R1 0.900 0.425 0.847 0.464 1.268 0.496 1.236
C21orf66_G_1_f C21orf66_G_r 0.910 0.429 0.434 1.068 1.402 0.803 0.390 1.405
CASC3_G_f CASC3_G_r 0.570 0.269 0.638 1.828 0.384 0.788
CCAR1_G_1_f CCAR1_G_r 2.101 0.992 1.251 0.734 0.700 0.457 0.926 1.916
CCDC94.G1.F1 CCDC94.G1.R1 0.076 0.036 0.256 2.244 0.221 0.536
CDC5L for1 CDC5L rev1 0.027 0.013 0.008 1.000
CIR_G_1_f CIR_G_r 0.281 1.914 0.086
CLNS1A_G_1_f CLNS1A_G_r 1.344 0.635 0.408 0.230 0.865 1.799 0.527 0.809
CROP_G_1_f CROP_G_r 0.131 0.062 0.061 1.644 1.758 0.310 0.287
CWC22_G_1_f CWC22_G_r 1.342 0.634 1.169 1.737 1.442 0.635 0.286 1.637
DDX17_G_1_f DDX17_G_r 0.339 0.160 0.173 0.427 1.573 1.802 0.153 0.472
DDX39_G_1_f DDX39_G_r 1.034 2.228 1.918 206.528 0.288 1.712
DDX3X_G_f DDX3X_G_r 1.163 0.549 0.628 0.295 1.104 0.581 1.315
DDX41_G_f DDX41_G_r 6.694 3.161 2.896 1.000
DDX46_G_f DDX46_G_r 1.944 0.918 1.828 1.107 0.893
QPCR Relative Expression Quantification (grouped) : Summary

Expression Table

ID Name
1 OVC S
2 OVN S
Primer Pair S1 p1 q1 S2 p2 q2
A2BP1_G_f A2BP1_G_r 1.225 1.000
AFF2_G_1_f AFF2_G_r 3.491 1.29e-01 1.000
BCAS2_G_f BCAS2_G_r 3.612 2.36e-01 1.000
BRUNOL6_G_3_f BRUNOL6_G_3_r 0.262 3.95e-01 1.000
BUD13_G_f BUD13_G_1_r 0.550 1.89e-01 1.000
C19orf29.G1.F1 C19orf29.G1.R1 1.002 9.97e-01 1.000
C21orf66_G_1_f C21orf66_G_r 0.657 2.82e-01 1.000
CASC3_G_f CASC3_G_r 0.401 2.96e-01 1.000
CCAR1_G_1_f CCAR1_G_r 1.159 7.11e-01 1.000
CCDC94.G1.F1 CCDC94.G1.R1 0.311 3.89e-01 1.000
CDC5L for1 CDC5L rev1 1.000
CIR_G_1_f CIR_G_r 1.260 8.64e-01 1.000
CLNS1A_G_1_f CLNS1A_G_r 0.589 2.76e-01 1.000
CROP_G_1_f CROP_G_r 0.106 1.14e-01 1.000
CWC22_G_1_f CWC22_G_r 1.237 5.50e-01 1.000
DDX17_G_1_f DDX17_G_r 0.244 1.58e-01 1.000
DDX39_G_1_f DDX39_G_r 43.485 3.56e-01 1.000
DDX3X_G_f DDX3X_G_r 0.653 2.59e-01 1.000
DDX41_G_f DDX41_G_r 5.901 1.000
DDX46_G_f DDX46_G_r 1.540 1.03e-01 1.000
QPCR Reference Gene Page : .

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