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2. Breast Validation Screen 
Gene primer pair list : Primer pairs
Forward Reverse Occurences View
TLK1.u.f.12 TLK1.u.r.10 51 View
TLK1.u.f.12 TLK1.u.r.11 51 View
Transcript map : TLK1
Exon
Length: 587
Transcript: TLK1.aAug05
Exon
Length: 119
Transcript: TLK1.aAug05
Exon
Length: 72
Transcript: TLK1.aAug05
Exon
Length: 76
Transcript: TLK1.aAug05
Exon
Length: 63
Transcript: TLK1.aAug05
Exon
Length: 47
Transcript: TLK1.aAug05
Exon
Length: 96
Transcript: TLK1.aAug05
Exon
Length: 90
Transcript: TLK1.aAug05
Exon
Length: 93
Transcript: TLK1.aAug05
Exon
Length: 111
Transcript: TLK1.aAug05
Exon
Length: 137
Transcript: TLK1.aAug05
Exon
Length: 189
Transcript: TLK1.aAug05
Exon
Length: 67
Transcript: TLK1.aAug05
Exon
Length: 98
Transcript: TLK1.aAug05
Exon
Length: 82
Transcript: TLK1.aAug05
Exon
Length: 92
Transcript: TLK1.aAug05
Exon
Length: 90
Transcript: TLK1.aAug05
Exon
Length: 170
Transcript: TLK1.aAug05
Exon
Length: 136
Transcript: TLK1.aAug05
Exon
Length: 112
Transcript: TLK1.aAug05
Exon
Length: 108
Transcript: TLK1.aAug05
Exon
Length: 3134
Transcript: TLK1.aAug05
Intron
Length: 42432
Transcript: TLK1.aAug05
Intron
Length: 34886
Transcript: TLK1.aAug05
Intron
Length: 21624
Transcript: TLK1.aAug05
Intron
Length: 1094
Transcript: TLK1.aAug05
Intron
Length: 1726
Transcript: TLK1.aAug05
Intron
Length: 1584
Transcript: TLK1.aAug05
Intron
Length: 1298
Transcript: TLK1.aAug05
Intron
Length: 1229
Transcript: TLK1.aAug05
Intron
Length: 3586
Transcript: TLK1.aAug05
Intron
Length: 113
Transcript: TLK1.aAug05
Intron
Length: 3451
Transcript: TLK1.aAug05
Intron
Length: 17768
Transcript: TLK1.aAug05
Intron
Length: 13390
Transcript: TLK1.aAug05
Intron
Length: 3396
Transcript: TLK1.aAug05
Intron
Length: 4309
Transcript: TLK1.aAug05
Intron
Length: 82
Transcript: TLK1.aAug05
Intron
Length: 156
Transcript: TLK1.aAug05
Intron
Length: 191
Transcript: TLK1.aAug05
Intron
Length: 8323
Transcript: TLK1.aAug05
Intron
Length: 951
Transcript: TLK1.aAug05
Intron
Length: 2696
Transcript: TLK1.aAug05
Reaction
Expected peak: 205
Associated peak: 218,219,220,221,222
Repeated 51 time
Molarity: 3.75M
Forward: TLK1.u.f.12
Reverse: TLK1.u.r.10
Reaction
Expected peak: 278
Associated peak: 282,283,284,285,286,287,288,289
Repeated 51 time
Molarity: 8.98M
Forward: TLK1.u.f.12
Reverse: TLK1.u.r.11
Exon
Length: 292
Transcript: TLK1.bAug05
Exon
Length: 119
Transcript: TLK1.bAug05
Exon
Length: 72
Transcript: TLK1.bAug05
Exon
Length: 76
Transcript: TLK1.bAug05
Exon
Length: 47
Transcript: TLK1.bAug05
Exon
Length: 96
Transcript: TLK1.bAug05
Exon
Length: 90
Transcript: TLK1.bAug05
Exon
Length: 93
Transcript: TLK1.bAug05
Exon
Length: 111
Transcript: TLK1.bAug05
Exon
Length: 137
Transcript: TLK1.bAug05
Exon
Length: 189
Transcript: TLK1.bAug05
Exon
Length: 67
Transcript: TLK1.bAug05
Exon
Length: 98
Transcript: TLK1.bAug05
Exon
Length: 82
Transcript: TLK1.bAug05
Exon
Length: 92
Transcript: TLK1.bAug05
Exon
Length: 90
Transcript: TLK1.bAug05
Exon
Length: 170
Transcript: TLK1.bAug05
Exon
Length: 136
Transcript: TLK1.bAug05
Exon
Length: 112
Transcript: TLK1.bAug05
Exon
Length: 108
Transcript: TLK1.bAug05
Exon
Length: 1904
Transcript: TLK1.bAug05
Intron
Length: 42432
Transcript: TLK1.bAug05
Intron
Length: 34886
Transcript: TLK1.bAug05
Intron
Length: 21624
Transcript: TLK1.bAug05
Intron
Length: 2883
Transcript: TLK1.bAug05
Intron
Length: 1584
Transcript: TLK1.bAug05
Intron
Length: 1298
Transcript: TLK1.bAug05
Intron
Length: 1229
Transcript: TLK1.bAug05
Intron
Length: 3586
Transcript: TLK1.bAug05
Intron
Length: 113
Transcript: TLK1.bAug05
Intron
Length: 3451
Transcript: TLK1.bAug05
Intron
Length: 17768
Transcript: TLK1.bAug05
Intron
Length: 13390
Transcript: TLK1.bAug05
Intron
Length: 3396
Transcript: TLK1.bAug05
Intron
Length: 4309
Transcript: TLK1.bAug05
Intron
Length: 82
Transcript: TLK1.bAug05
Intron
Length: 156
Transcript: TLK1.bAug05
Intron
Length: 191
Transcript: TLK1.bAug05
Intron
Length: 8323
Transcript: TLK1.bAug05
Intron
Length: 951
Transcript: TLK1.bAug05
Intron
Length: 2696
Transcript: TLK1.bAug05
Reaction
Expected peak: 142
Associated peak: 144,145,146,147,148,149
Repeated 51 time
Molarity: 3.26M
Forward: TLK1.u.f.12
Reverse: TLK1.u.r.10
Reaction
Expected peak: 215
Associated peak: 221,222,223,224
Repeated 51 time
Molarity: 23.61M
Forward: TLK1.u.f.12
Reverse: TLK1.u.r.11
Exon
Length: 170
Transcript: TLK1.cAug05
Exon
Length: 119
Transcript: TLK1.cAug05
Exon
Length: 72
Transcript: TLK1.cAug05
Exon
Length: 76
Transcript: TLK1.cAug05
Exon
Length: 47
Transcript: TLK1.cAug05
Exon
Length: 96
Transcript: TLK1.cAug05
Exon
Length: 90
Transcript: TLK1.cAug05
Exon
Length: 93
Transcript: TLK1.cAug05
Exon
Length: 111
Transcript: TLK1.cAug05
Exon
Length: 137
Transcript: TLK1.cAug05
Exon
Length: 189
Transcript: TLK1.cAug05
Exon
Length: 67
Transcript: TLK1.cAug05
Exon
Length: 98
Transcript: TLK1.cAug05
Exon
Length: 82
Transcript: TLK1.cAug05
Exon
Length: 92
Transcript: TLK1.cAug05
Exon
Length: 90
Transcript: TLK1.cAug05
Exon
Length: 170
Transcript: TLK1.cAug05
Exon
Length: 136
Transcript: TLK1.cAug05
Exon
Length: 112
Transcript: TLK1.cAug05
Exon
Length: 108
Transcript: TLK1.cAug05
Exon
Length: 450
Transcript: TLK1.cAug05
Intron
Length: 113287
Transcript: TLK1.cAug05
Intron
Length: 34886
Transcript: TLK1.cAug05
Intron
Length: 21624
Transcript: TLK1.cAug05
Intron
Length: 2883
Transcript: TLK1.cAug05
Intron
Length: 1584
Transcript: TLK1.cAug05
Intron
Length: 1298
Transcript: TLK1.cAug05
Intron
Length: 1229
Transcript: TLK1.cAug05
Intron
Length: 3586
Transcript: TLK1.cAug05
Intron
Length: 113
Transcript: TLK1.cAug05
Intron
Length: 3451
Transcript: TLK1.cAug05
Intron
Length: 17768
Transcript: TLK1.cAug05
Intron
Length: 13390
Transcript: TLK1.cAug05
Intron
Length: 3396
Transcript: TLK1.cAug05
Intron
Length: 4309
Transcript: TLK1.cAug05
Intron
Length: 82
Transcript: TLK1.cAug05
Intron
Length: 156
Transcript: TLK1.cAug05
Intron
Length: 191
Transcript: TLK1.cAug05
Intron
Length: 8323
Transcript: TLK1.cAug05
Intron
Length: 951
Transcript: TLK1.cAug05
Intron
Length: 2696
Transcript: TLK1.cAug05
Reaction
Expected peak: 142
Associated peak: 144,145,146,147,148,149
Repeated 51 time
Molarity: 3.26M
Forward: TLK1.u.f.12
Reverse: TLK1.u.r.10
Reaction
Expected peak: 215
Associated peak: 221,222,223,224
Repeated 51 time
Molarity: 23.61M
Forward: TLK1.u.f.12
Reverse: TLK1.u.r.11
Exon
Length: 611
Transcript: TLK1.dAug05
Exon
Length: 119
Transcript: TLK1.dAug05
Exon
Length: 72
Transcript: TLK1.dAug05
Exon
Length: 76
Transcript: TLK1.dAug05
Exon
Length: 47
Transcript: TLK1.dAug05
Exon
Length: 96
Transcript: TLK1.dAug05
Exon
Length: 90
Transcript: TLK1.dAug05
Exon
Length: 93
Transcript: TLK1.dAug05
Exon
Length: 111
Transcript: TLK1.dAug05
Exon
Length: 137
Transcript: TLK1.dAug05
Exon
Length: 189
Transcript: TLK1.dAug05
Exon
Length: 67
Transcript: TLK1.dAug05
Exon
Length: 258
Transcript: TLK1.dAug05
Exon
Length: 98
Transcript: TLK1.dAug05
Exon
Length: 82
Transcript: TLK1.dAug05
Exon
Length: 92
Transcript: TLK1.dAug05
Exon
Length: 90
Transcript: TLK1.dAug05
Exon
Length: 170
Transcript: TLK1.dAug05
Exon
Length: 136
Transcript: TLK1.dAug05
Exon
Length: 112
Transcript: TLK1.dAug05
Exon
Length: 37
Transcript: TLK1.dAug05
Intron
Length: 42432
Transcript: TLK1.dAug05
Intron
Length: 34886
Transcript: TLK1.dAug05
Intron
Length: 21624
Transcript: TLK1.dAug05
Intron
Length: 2883
Transcript: TLK1.dAug05
Intron
Length: 1584
Transcript: TLK1.dAug05
Intron
Length: 1298
Transcript: TLK1.dAug05
Intron
Length: 1229
Transcript: TLK1.dAug05
Intron
Length: 3586
Transcript: TLK1.dAug05
Intron
Length: 113
Transcript: TLK1.dAug05
Intron
Length: 3451
Transcript: TLK1.dAug05
Intron
Length: 17768
Transcript: TLK1.dAug05
Intron
Length: 5209
Transcript: TLK1.dAug05
Intron
Length: 7923
Transcript: TLK1.dAug05
Intron
Length: 3396
Transcript: TLK1.dAug05
Intron
Length: 4309
Transcript: TLK1.dAug05
Intron
Length: 82
Transcript: TLK1.dAug05
Intron
Length: 156
Transcript: TLK1.dAug05
Intron
Length: 191
Transcript: TLK1.dAug05
Intron
Length: 8323
Transcript: TLK1.dAug05
Intron
Length: 951
Transcript: TLK1.dAug05
Reaction
Expected peak: 142
Associated peak: 144,145,146,147,148,149
Repeated 51 time
Molarity: 3.26M
Forward: TLK1.u.f.12
Reverse: TLK1.u.r.10
Reaction
Expected peak: 215
Associated peak: 221,222,223,224
Repeated 51 time
Molarity: 23.61M
Forward: TLK1.u.f.12
Reverse: TLK1.u.r.11
Exon
Length: 204
Transcript: TLK1.eAug05
Exon
Length: 72
Transcript: TLK1.eAug05
Exon
Length: 76
Transcript: TLK1.eAug05
Exon
Length: 47
Transcript: TLK1.eAug05
Exon
Length: 96
Transcript: TLK1.eAug05
Exon
Length: 90
Transcript: TLK1.eAug05
Exon
Length: 93
Transcript: TLK1.eAug05
Exon
Length: 70
Transcript: TLK1.eAug05
Intron
Length: 8761
Transcript: TLK1.eAug05
Intron
Length: 21624
Transcript: TLK1.eAug05
Intron
Length: 2883
Transcript: TLK1.eAug05
Intron
Length: 1584
Transcript: TLK1.eAug05
Intron
Length: 1298
Transcript: TLK1.eAug05
Intron
Length: 1229
Transcript: TLK1.eAug05
Intron
Length: 3586
Transcript: TLK1.eAug05
Reaction
Expected peak: 142
Associated peak: 144,145,146,147,148,149
Repeated 51 time
Molarity: 3.26M
Forward: TLK1.u.f.12
Reverse: TLK1.u.r.10
Reaction
Expected peak: 215
Associated peak: 221,222,223,224
Repeated 51 time
Molarity: 23.61M
Forward: TLK1.u.f.12
Reverse: TLK1.u.r.11
Exon
Length: 1369
Transcript: TLK1.fAug05
Exon
Length: 111
Transcript: TLK1.fAug05
Exon
Length: 137
Transcript: TLK1.fAug05
Exon
Length: 1114
Transcript: TLK1.fAug05
Intron
Length: 3586
Transcript: TLK1.fAug05
Intron
Length: 113
Transcript: TLK1.fAug05
Intron
Length: 3451
Transcript: TLK1.fAug05
Exon
Length: 396
Transcript: TLK1.gAug05
Exon
Length: 119
Transcript: TLK1.gAug05
Exon
Length: 72
Transcript: TLK1.gAug05
Exon
Length: 69
Transcript: TLK1.gAug05
Exon
Length: 76
Transcript: TLK1.gAug05
Exon
Length: 47
Transcript: TLK1.gAug05
Exon
Length: 96
Transcript: TLK1.gAug05
Exon
Length: 43
Transcript: TLK1.gAug05
Intron
Length: 42432
Transcript: TLK1.gAug05
Intron
Length: 34886
Transcript: TLK1.gAug05
Intron
Length: 1072
Transcript: TLK1.gAug05
Intron
Length: 20483
Transcript: TLK1.gAug05
Intron
Length: 2883
Transcript: TLK1.gAug05
Intron
Length: 1584
Transcript: TLK1.gAug05
Intron
Length: 1298
Transcript: TLK1.gAug05
Reaction
Expected peak: 211
Associated peak: 218,219,220,221,222
Repeated 51 time
Molarity: 3.75M
Forward: TLK1.u.f.12
Reverse: TLK1.u.r.10
Reaction
Expected peak: 284
Associated peak: 282,283,284,285,286,287,288,289
Repeated 51 time
Molarity: 8.98M
Forward: TLK1.u.f.12
Reverse: TLK1.u.r.11
Exon
Length: 633
Transcript: TLK1.hAug05
Exon
Length: 119
Transcript: TLK1.hAug05
Exon
Length: 72
Transcript: TLK1.hAug05
Exon
Length: 56
Transcript: TLK1.hAug05
Intron
Length: 42432
Transcript: TLK1.hAug05
Intron
Length: 34886
Transcript: TLK1.hAug05
Intron
Length: 9859
Transcript: TLK1.hAug05
Exon
Length: 146
Transcript: TLK1.iAug05
Exon
Length: 119
Transcript: TLK1.iAug05
Exon
Length: 182
Transcript: TLK1.iAug05
Intron
Length: 42432
Transcript: TLK1.iAug05
Intron
Length: 25511
Transcript: TLK1.iAug05
Exon
Length: 367
Transcript: TLK1.jAug05
Exon
Length: 107
Transcript: TLK1.jAug05
Exon
Length: 119
Transcript: TLK1.jAug05
Intron
Length: 64335
Transcript: TLK1.jAug05
Intron
Length: 48845
Transcript: TLK1.jAug05
Exon
Length: 766
Transcript: TLK1.shed1.aAug05
Exon
Length: 627
Transcript: TLK1.shed2.aAug05
Exon
Length: 342
Transcript: TLK1.shed3.aAug05
Exon
Length: 430
Transcript: TLK1.shed4.aAug05
TLK1.u.f.12
TGGAAGCTTGGGATCTTTAAGT
TLK1.u.r.11
AACGGATTGCAGGAGGTATG
TLK1.u.r.10
TTTGTGGCCACGTCCC

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