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2. Breast Validation Screen 
Gene primer pair list : Primer pairs
Forward Reverse Occurences View
TUBA1.F5 TUBA1.R5 51 View
Transcript map : TUBA1
Exon
Length: 91
Transcript: TUBA1.aAug05
Exon
Length: 223
Transcript: TUBA1.aAug05
Exon
Length: 149
Transcript: TUBA1.aAug05
Exon
Length: 1883
Transcript: TUBA1.aAug05
Intron
Length: 1625
Transcript: TUBA1.aAug05
Intron
Length: 294
Transcript: TUBA1.aAug05
Intron
Length: 241
Transcript: TUBA1.aAug05
Reaction
Expected peak: 324
Associated peak: 335,336,337,338,339,340,341,342,343,344,347,350
Repeated 51 time
Molarity: 41.76M
Forward: TUBA1.F5
Reverse: TUBA1.R5
Exon
Length: 428
Transcript: TUBA1.bAug05
Exon
Length: 223
Transcript: TUBA1.bAug05
Exon
Length: 149
Transcript: TUBA1.bAug05
Exon
Length: 682
Transcript: TUBA1.bAug05
Intron
Length: 25583
Transcript: TUBA1.bAug05
Intron
Length: 294
Transcript: TUBA1.bAug05
Intron
Length: 241
Transcript: TUBA1.bAug05
Exon
Length: 469
Transcript: TUBA1.cAug05
Exon
Length: 223
Transcript: TUBA1.cAug05
Exon
Length: 149
Transcript: TUBA1.cAug05
Exon
Length: 246
Transcript: TUBA1.cAug05
Intron
Length: 1864
Transcript: TUBA1.cAug05
Intron
Length: 294
Transcript: TUBA1.cAug05
Intron
Length: 241
Transcript: TUBA1.cAug05
Exon
Length: 105
Transcript: TUBA1.dAug05
Exon
Length: 223
Transcript: TUBA1.dAug05
Exon
Length: 149
Transcript: TUBA1.dAug05
Exon
Length: 446
Transcript: TUBA1.dAug05
Intron
Length: 978
Transcript: TUBA1.dAug05
Intron
Length: 294
Transcript: TUBA1.dAug05
Intron
Length: 241
Transcript: TUBA1.dAug05
Exon
Length: 33
Transcript: TUBA1.eAug05
Exon
Length: 149
Transcript: TUBA1.eAug05
Exon
Length: 552
Transcript: TUBA1.eAug05
Intron
Length: 2142
Transcript: TUBA1.eAug05
Intron
Length: 241
Transcript: TUBA1.eAug05
Reaction
Expected peak: 101
Associated peak: 104,105,106,107,108,109
Repeated 51 time
Molarity: 11.27M
Forward: TUBA1.F5
Reverse: TUBA1.R5
Exon
Length: 150
Transcript: TUBA1.fAug05
Exon
Length: 223
Transcript: TUBA1.fAug05
Exon
Length: 149
Transcript: TUBA1.fAug05
Exon
Length: 82
Transcript: TUBA1.fAug05
Intron
Length: 765
Transcript: TUBA1.fAug05
Intron
Length: 294
Transcript: TUBA1.fAug05
Intron
Length: 241
Transcript: TUBA1.fAug05
Exon
Length: 428
Transcript: TUBA1.gAug05
Exon
Length: 552
Transcript: TUBA1.gAug05
Intron
Length: 26490
Transcript: TUBA1.gAug05
Exon
Length: 61
Transcript: TUBA1.hAug05
Exon
Length: 223
Transcript: TUBA1.hAug05
Exon
Length: 145
Transcript: TUBA1.hAug05
Exon
Length: 100
Transcript: TUBA1.hAug05
Intron
Length: 1625
Transcript: TUBA1.hAug05
Intron
Length: 294
Transcript: TUBA1.hAug05
Intron
Length: 245
Transcript: TUBA1.hAug05
Reaction
Expected peak: 324
Associated peak: 335,336,337,338,339,340,341,342,343,344,347,350
Repeated 51 time
Molarity: 41.76M
Forward: TUBA1.F5
Reverse: TUBA1.R5
Exon
Length: 103
Transcript: TUBA1.iAug05
Exon
Length: 679
Transcript: TUBA1.iAug05
Intron
Length: 294
Transcript: TUBA1.iAug05
Exon
Length: 49
Transcript: TUBA1.jAug05
Exon
Length: 692
Transcript: TUBA1.jAug05
Intron
Length: 1625
Transcript: TUBA1.jAug05
Reaction
Expected peak: 618
Associated peak: None
Repeated 51 time
Molarity: 0.00M
Forward: TUBA1.F5
Reverse: TUBA1.R5
TUBA1.R5
CTCTTTCCCAGTGATGAGCTG
TUBA1.F5
GACTCAACGTGAGACGCACC

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