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2. Breast Validation Screen 
Transcript map : POLB
Exon
Length: 231
Transcript: POLB.aAug05
Exon
Length: 58
Transcript: POLB.aAug05
Exon
Length: 67
Transcript: POLB.aAug05
Exon
Length: 75
Transcript: POLB.aAug05
Exon
Length: 59
Transcript: POLB.aAug05
Exon
Length: 50
Transcript: POLB.aAug05
Exon
Length: 52
Transcript: POLB.aAug05
Exon
Length: 55
Transcript: POLB.aAug05
Exon
Length: 73
Transcript: POLB.aAug05
Exon
Length: 71
Transcript: POLB.aAug05
Exon
Length: 87
Transcript: POLB.aAug05
Exon
Length: 65
Transcript: POLB.aAug05
Exon
Length: 140
Transcript: POLB.aAug05
Exon
Length: 250
Transcript: POLB.aAug05
Intron
Length: 326
Transcript: POLB.aAug05
Intron
Length: 5883
Transcript: POLB.aAug05
Intron
Length: 3996
Transcript: POLB.aAug05
Intron
Length: 916
Transcript: POLB.aAug05
Intron
Length: 2453
Transcript: POLB.aAug05
Intron
Length: 2947
Transcript: POLB.aAug05
Intron
Length: 1601
Transcript: POLB.aAug05
Intron
Length: 96
Transcript: POLB.aAug05
Intron
Length: 3902
Transcript: POLB.aAug05
Intron
Length: 1246
Transcript: POLB.aAug05
Intron
Length: 6572
Transcript: POLB.aAug05
Intron
Length: 513
Transcript: POLB.aAug05
Intron
Length: 1574
Transcript: POLB.aAug05
Reaction
Expected peak: 213
Associated peak: 225,226,227,228,229
Repeated 51 time
Molarity: 17.29M
Forward: POLB.F8
Reverse: POLB.R14
Reaction
Expected peak: 271
Associated peak: 277,278,279,280
Repeated 51 time
Molarity: 22.43M
Forward: POLB.F7
Reverse: POLB.R13
Exon
Length: 204
Transcript: POLB.bAug05
Exon
Length: 58
Transcript: POLB.bAug05
Exon
Length: 67
Transcript: POLB.bAug05
Exon
Length: 75
Transcript: POLB.bAug05
Exon
Length: 59
Transcript: POLB.bAug05
Exon
Length: 50
Transcript: POLB.bAug05
Exon
Length: 105
Transcript: POLB.bAug05
Exon
Length: 52
Transcript: POLB.bAug05
Exon
Length: 55
Transcript: POLB.bAug05
Exon
Length: 73
Transcript: POLB.bAug05
Exon
Length: 71
Transcript: POLB.bAug05
Exon
Length: 87
Transcript: POLB.bAug05
Exon
Length: 255
Transcript: POLB.bAug05
Intron
Length: 326
Transcript: POLB.bAug05
Intron
Length: 5883
Transcript: POLB.bAug05
Intron
Length: 3996
Transcript: POLB.bAug05
Intron
Length: 916
Transcript: POLB.bAug05
Intron
Length: 2453
Transcript: POLB.bAug05
Intron
Length: 1848
Transcript: POLB.bAug05
Intron
Length: 994
Transcript: POLB.bAug05
Intron
Length: 1601
Transcript: POLB.bAug05
Intron
Length: 96
Transcript: POLB.bAug05
Intron
Length: 3902
Transcript: POLB.bAug05
Intron
Length: 1246
Transcript: POLB.bAug05
Intron
Length: 8864
Transcript: POLB.bAug05
Reaction
Expected peak: 213
Associated peak: 225,226,227,228,229
Repeated 51 time
Molarity: 17.29M
Forward: POLB.F8
Reverse: POLB.R14
Reaction
Expected peak: 271
Associated peak: 277,278,279,280
Repeated 51 time
Molarity: 22.43M
Forward: POLB.F7
Reverse: POLB.R13
Exon
Length: 188
Transcript: POLB.cAug05
Exon
Length: 58
Transcript: POLB.cAug05
Exon
Length: 67
Transcript: POLB.cAug05
Exon
Length: 75
Transcript: POLB.cAug05
Exon
Length: 59
Transcript: POLB.cAug05
Exon
Length: 50
Transcript: POLB.cAug05
Exon
Length: 52
Transcript: POLB.cAug05
Exon
Length: 55
Transcript: POLB.cAug05
Exon
Length: 73
Transcript: POLB.cAug05
Exon
Length: 71
Transcript: POLB.cAug05
Exon
Length: 65
Transcript: POLB.cAug05
Exon
Length: 140
Transcript: POLB.cAug05
Exon
Length: 246
Transcript: POLB.cAug05
Intron
Length: 326
Transcript: POLB.cAug05
Intron
Length: 5883
Transcript: POLB.cAug05
Intron
Length: 3996
Transcript: POLB.cAug05
Intron
Length: 916
Transcript: POLB.cAug05
Intron
Length: 2453
Transcript: POLB.cAug05
Intron
Length: 2947
Transcript: POLB.cAug05
Intron
Length: 1601
Transcript: POLB.cAug05
Intron
Length: 96
Transcript: POLB.cAug05
Intron
Length: 3902
Transcript: POLB.cAug05
Intron
Length: 7905
Transcript: POLB.cAug05
Intron
Length: 513
Transcript: POLB.cAug05
Intron
Length: 1574
Transcript: POLB.cAug05
Reaction
Expected peak: 213
Associated peak: 225,226,227,228,229
Repeated 51 time
Molarity: 17.29M
Forward: POLB.F8
Reverse: POLB.R14
Reaction
Expected peak: 271
Associated peak: 277,278,279,280
Repeated 51 time
Molarity: 22.43M
Forward: POLB.F7
Reverse: POLB.R13
Exon
Length: 221
Transcript: POLB.dAug05
Exon
Length: 67
Transcript: POLB.dAug05
Exon
Length: 75
Transcript: POLB.dAug05
Exon
Length: 59
Transcript: POLB.dAug05
Exon
Length: 50
Transcript: POLB.dAug05
Exon
Length: 52
Transcript: POLB.dAug05
Exon
Length: 55
Transcript: POLB.dAug05
Exon
Length: 73
Transcript: POLB.dAug05
Exon
Length: 71
Transcript: POLB.dAug05
Exon
Length: 87
Transcript: POLB.dAug05
Exon
Length: 65
Transcript: POLB.dAug05
Exon
Length: 30
Transcript: POLB.dAug05
Intron
Length: 6267
Transcript: POLB.dAug05
Intron
Length: 3996
Transcript: POLB.dAug05
Intron
Length: 916
Transcript: POLB.dAug05
Intron
Length: 2453
Transcript: POLB.dAug05
Intron
Length: 2947
Transcript: POLB.dAug05
Intron
Length: 1601
Transcript: POLB.dAug05
Intron
Length: 96
Transcript: POLB.dAug05
Intron
Length: 3902
Transcript: POLB.dAug05
Intron
Length: 1246
Transcript: POLB.dAug05
Intron
Length: 6572
Transcript: POLB.dAug05
Intron
Length: 513
Transcript: POLB.dAug05
Reaction
Expected peak: 155
Associated peak: 167,168,169
Repeated 51 time
Molarity: 15.39M
Forward: POLB.F8
Reverse: POLB.R14
Reaction
Expected peak: 213
Associated peak: 224,225,226,227
Repeated 51 time
Molarity: 18.84M
Forward: POLB.F7
Reverse: POLB.R13
Exon
Length: 60
Transcript: POLB.eAug05
Exon
Length: 50
Transcript: POLB.eAug05
Exon
Length: 52
Transcript: POLB.eAug05
Exon
Length: 55
Transcript: POLB.eAug05
Exon
Length: 73
Transcript: POLB.eAug05
Exon
Length: 71
Transcript: POLB.eAug05
Exon
Length: 87
Transcript: POLB.eAug05
Exon
Length: 65
Transcript: POLB.eAug05
Exon
Length: 140
Transcript: POLB.eAug05
Intron
Length: 7499
Transcript: POLB.eAug05
Intron
Length: 2947
Transcript: POLB.eAug05
Intron
Length: 1601
Transcript: POLB.eAug05
Intron
Length: 96
Transcript: POLB.eAug05
Intron
Length: 3902
Transcript: POLB.eAug05
Intron
Length: 1246
Transcript: POLB.eAug05
Intron
Length: 6572
Transcript: POLB.eAug05
Intron
Length: 513
Transcript: POLB.eAug05
Exon
Length: 13
Transcript: POLB.fAug05
Exon
Length: 105
Transcript: POLB.fAug05
Exon
Length: 52
Transcript: POLB.fAug05
Exon
Length: 55
Transcript: POLB.fAug05
Exon
Length: 73
Transcript: POLB.fAug05
Exon
Length: 71
Transcript: POLB.fAug05
Exon
Length: 65
Transcript: POLB.fAug05
Exon
Length: 140
Transcript: POLB.fAug05
Intron
Length: 1848
Transcript: POLB.fAug05
Intron
Length: 994
Transcript: POLB.fAug05
Intron
Length: 1601
Transcript: POLB.fAug05
Intron
Length: 96
Transcript: POLB.fAug05
Intron
Length: 3902
Transcript: POLB.fAug05
Intron
Length: 7905
Transcript: POLB.fAug05
Intron
Length: 513
Transcript: POLB.fAug05
Exon
Length: 221
Transcript: POLB.gAug05
Exon
Length: 67
Transcript: POLB.gAug05
Exon
Length: 75
Transcript: POLB.gAug05
Exon
Length: 59
Transcript: POLB.gAug05
Exon
Length: 50
Transcript: POLB.gAug05
Exon
Length: 52
Transcript: POLB.gAug05
Exon
Length: 55
Transcript: POLB.gAug05
Exon
Length: 73
Transcript: POLB.gAug05
Exon
Length: 92
Transcript: POLB.gAug05
Intron
Length: 6267
Transcript: POLB.gAug05
Intron
Length: 3996
Transcript: POLB.gAug05
Intron
Length: 916
Transcript: POLB.gAug05
Intron
Length: 2453
Transcript: POLB.gAug05
Intron
Length: 2947
Transcript: POLB.gAug05
Intron
Length: 1601
Transcript: POLB.gAug05
Intron
Length: 96
Transcript: POLB.gAug05
Intron
Length: 3703
Transcript: POLB.gAug05
Reaction
Expected peak: 155
Associated peak: 167,168,169
Repeated 51 time
Molarity: 15.39M
Forward: POLB.F8
Reverse: POLB.R14
Reaction
Expected peak: 213
Associated peak: 224,225,226,227
Repeated 51 time
Molarity: 18.84M
Forward: POLB.F7
Reverse: POLB.R13
Exon
Length: 175
Transcript: POLB.hAug05
Exon
Length: 67
Transcript: POLB.hAug05
Exon
Length: 75
Transcript: POLB.hAug05
Exon
Length: 78
Transcript: POLB.hAug05
Exon
Length: 50
Transcript: POLB.hAug05
Exon
Length: 52
Transcript: POLB.hAug05
Exon
Length: 55
Transcript: POLB.hAug05
Exon
Length: 73
Transcript: POLB.hAug05
Exon
Length: 71
Transcript: POLB.hAug05
Exon
Length: 79
Transcript: POLB.hAug05
Intron
Length: 6267
Transcript: POLB.hAug05
Intron
Length: 3996
Transcript: POLB.hAug05
Intron
Length: 897
Transcript: POLB.hAug05
Intron
Length: 2453
Transcript: POLB.hAug05
Intron
Length: 2947
Transcript: POLB.hAug05
Intron
Length: 1601
Transcript: POLB.hAug05
Intron
Length: 96
Transcript: POLB.hAug05
Intron
Length: 3902
Transcript: POLB.hAug05
Intron
Length: 1246
Transcript: POLB.hAug05
Reaction
Expected peak: 155
Associated peak: 167,168,169
Repeated 51 time
Molarity: 15.39M
Forward: POLB.F8
Reverse: POLB.R14
Reaction
Expected peak: 213
Associated peak: 224,225,226,227
Repeated 51 time
Molarity: 18.84M
Forward: POLB.F7
Reverse: POLB.R13
Exon
Length: 113
Transcript: POLB.iAug05
Exon
Length: 55
Transcript: POLB.iAug05
Exon
Length: 73
Transcript: POLB.iAug05
Exon
Length: 1387
Transcript: POLB.iAug05
Intron
Length: 1601
Transcript: POLB.iAug05
Intron
Length: 96
Transcript: POLB.iAug05
Intron
Length: 3902
Transcript: POLB.iAug05
Exon
Length: 448
Transcript: POLB.jAug05
Exon
Length: 140
Transcript: POLB.jAug05
Exon
Length: 251
Transcript: POLB.jAug05
Intron
Length: 513
Transcript: POLB.jAug05
Intron
Length: 1574
Transcript: POLB.jAug05
Exon
Length: 26
Transcript: POLB.kAug05
Exon
Length: 560
Transcript: POLB.kAug05
Intron
Length: 96
Transcript: POLB.kAug05
Exon
Length: 4828
Transcript: POLB.shed1.aAug05
Exon
Length: 426
Transcript: POLB.shed2.aAug05
Exon
Length: 724
Transcript: POLB.shed3.aAug05
POLB.F7
TCCAAGTCCTGGTACCTCCTT
POLB.R13
CAATTTCTTAGCTTCAGCTCCAC
POLB.F8
TCCCTGGTTCTGAACACTCTG
POLB.R14
GCTCCACTCTTTATTTTGTGTGG
Gene primer pair list : Primer pairs
Forward Reverse Occurences View
POLB.F7 POLB.R13 51 View
POLB.F8 POLB.R14 51 View

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