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2. Breast Validation Screen 
Gene primer pair list : Primer pairs
Forward Reverse Occurences View
WISP1.u.f.1 WISP1.u.r.4 51 View
WISP1.u.f.4 WISP1.u.r.4 51 View
Transcript map : WISP1
Exon
Length: 145
Transcript: WISP1.aAug05
Exon
Length: 280
Transcript: WISP1.aAug05
Exon
Length: 261
Transcript: WISP1.aAug05
Exon
Length: 194
Transcript: WISP1.aAug05
Exon
Length: 4280
Transcript: WISP1.aAug05
Intron
Length: 21650
Transcript: WISP1.aAug05
Intron
Length: 7437
Transcript: WISP1.aAug05
Intron
Length: 4548
Transcript: WISP1.aAug05
Intron
Length: 1827
Transcript: WISP1.aAug05
Reaction
Expected peak: 628
Associated peak: 658,662
Repeated 51 time
Molarity: 0.02M
Forward: WISP1.u.f.4
Reverse: WISP1.u.r.4
Reaction
Expected peak: 913
Associated peak: 884,895,900,905,929
Repeated 51 time
Molarity: 0.84M
Forward: WISP1.u.f.1
Reverse: WISP1.u.r.4
Exon
Length: 145
Transcript: WISP1.bAug05
Exon
Length: 280
Transcript: WISP1.bAug05
Exon
Length: 194
Transcript: WISP1.bAug05
Exon
Length: 399
Transcript: WISP1.bAug05
Intron
Length: 21650
Transcript: WISP1.bAug05
Intron
Length: 12246
Transcript: WISP1.bAug05
Intron
Length: 1827
Transcript: WISP1.bAug05
Reaction
Expected peak: 367
Associated peak: 415,416,417,418,419,420,421,423
Repeated 51 time
Molarity: 2.03M
Forward: WISP1.u.f.4
Reverse: WISP1.u.r.4
Reaction
Expected peak: 652
Associated peak: 624,632,644,650,651,655,656,660
Repeated 51 time
Molarity: 0.17M
Forward: WISP1.u.f.1
Reverse: WISP1.u.r.4
Exon
Length: 130
Transcript: WISP1.cAug05
Exon
Length: 194
Transcript: WISP1.cAug05
Exon
Length: 306
Transcript: WISP1.cAug05
Intron
Length: 34176
Transcript: WISP1.cAug05
Intron
Length: 1827
Transcript: WISP1.cAug05
Reaction
Expected peak: 372
Associated peak: 408,410,411,412,413,414,416
Repeated 51 time
Molarity: 0.45M
Forward: WISP1.u.f.1
Reverse: WISP1.u.r.4
Exon
Length: 175
Transcript: WISP1.dAug05
Exon
Length: 280
Transcript: WISP1.dAug05
Exon
Length: 306
Transcript: WISP1.dAug05
Intron
Length: 21650
Transcript: WISP1.dAug05
Intron
Length: 14267
Transcript: WISP1.dAug05
Reaction
Expected peak: 173
Associated peak: 195,196,197
Repeated 51 time
Molarity: 0.78M
Forward: WISP1.u.f.4
Reverse: WISP1.u.r.4
Reaction
Expected peak: 458
Associated peak: 471,473,474,476,477,478,479,493,495,496,498,500
Repeated 51 time
Molarity: 0.45M
Forward: WISP1.u.f.1
Reverse: WISP1.u.r.4
Exon
Length: 145
Transcript: WISP1.eAug05
Exon
Length: 399
Transcript: WISP1.eAug05
Intron
Length: 36197
Transcript: WISP1.eAug05
Reaction
Expected peak: 178
Associated peak: 194,195,196,197
Repeated 51 time
Molarity: 1.14M
Forward: WISP1.u.f.1
Reverse: WISP1.u.r.4
Exon
Length: 442
Transcript: WISP1.shed1.aAug05
WISP1.u.r.4
AAGTTCATGGATGCCTCTGG
WISP1.u.f.1
GAGAGGTGGTCGGATCCTCT
WISP1.u.f.4
GTAAGATGTGCGCTCAGCAG

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