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2. Breast Validation Screen 
Gene primer pair list : Primer pairs
Forward Reverse Occurences View
RAD52.u.f.15 RAD52.u.r.18 51 View
RAD52.u.f.18 RAD52.u.r.18 51 View
Transcript map : RAD52
Exon
Length: 81
Transcript: RAD52.aAug05
Exon
Length: 102
Transcript: RAD52.aAug05
Exon
Length: 102
Transcript: RAD52.aAug05
Exon
Length: 94
Transcript: RAD52.aAug05
Exon
Length: 68
Transcript: RAD52.aAug05
Exon
Length: 119
Transcript: RAD52.aAug05
Exon
Length: 76
Transcript: RAD52.aAug05
Exon
Length: 182
Transcript: RAD52.aAug05
Exon
Length: 140
Transcript: RAD52.aAug05
Exon
Length: 102
Transcript: RAD52.aAug05
Exon
Length: 228
Transcript: RAD52.aAug05
Exon
Length: 5203
Transcript: RAD52.aAug05
Intron
Length: 16525
Transcript: RAD52.aAug05
Intron
Length: 1653
Transcript: RAD52.aAug05
Intron
Length: 1075
Transcript: RAD52.aAug05
Intron
Length: 164
Transcript: RAD52.aAug05
Intron
Length: 2555
Transcript: RAD52.aAug05
Intron
Length: 1619
Transcript: RAD52.aAug05
Intron
Length: 8629
Transcript: RAD52.aAug05
Intron
Length: 155
Transcript: RAD52.aAug05
Intron
Length: 1811
Transcript: RAD52.aAug05
Intron
Length: 309
Transcript: RAD52.aAug05
Intron
Length: 441
Transcript: RAD52.aAug05
Reaction
Expected peak: 236
Associated peak: 245,246,247,248,249,254,255,256
Repeated 51 time
Molarity: 24.76M
Forward: RAD52.u.f.18
Reverse: RAD52.u.r.18
Reaction
Expected peak: 330
Associated peak: 341,342,343,344,345,346,347,348,349,350,351,352
Repeated 51 time
Molarity: 16.10M
Forward: RAD52.u.f.15
Reverse: RAD52.u.r.18
Exon
Length: 1032
Transcript: RAD52.bAug05
Exon
Length: 102
Transcript: RAD52.bAug05
Exon
Length: 102
Transcript: RAD52.bAug05
Exon
Length: 94
Transcript: RAD52.bAug05
Exon
Length: 68
Transcript: RAD52.bAug05
Exon
Length: 119
Transcript: RAD52.bAug05
Exon
Length: 76
Transcript: RAD52.bAug05
Exon
Length: 182
Transcript: RAD52.bAug05
Exon
Length: 140
Transcript: RAD52.bAug05
Exon
Length: 102
Transcript: RAD52.bAug05
Exon
Length: 228
Transcript: RAD52.bAug05
Exon
Length: 908
Transcript: RAD52.bAug05
Intron
Length: 56732
Transcript: RAD52.bAug05
Intron
Length: 1653
Transcript: RAD52.bAug05
Intron
Length: 1075
Transcript: RAD52.bAug05
Intron
Length: 164
Transcript: RAD52.bAug05
Intron
Length: 2555
Transcript: RAD52.bAug05
Intron
Length: 1619
Transcript: RAD52.bAug05
Intron
Length: 8629
Transcript: RAD52.bAug05
Intron
Length: 155
Transcript: RAD52.bAug05
Intron
Length: 1811
Transcript: RAD52.bAug05
Intron
Length: 309
Transcript: RAD52.bAug05
Intron
Length: 441
Transcript: RAD52.bAug05
Reaction
Expected peak: 236
Associated peak: 245,246,247,248,249,254,255,256
Repeated 51 time
Molarity: 24.76M
Forward: RAD52.u.f.18
Reverse: RAD52.u.r.18
Reaction
Expected peak: 330
Associated peak: 341,342,343,344,345,346,347,348,349,350,351,352
Repeated 51 time
Molarity: 16.10M
Forward: RAD52.u.f.15
Reverse: RAD52.u.r.18
Exon
Length: 40
Transcript: RAD52.cAug05
Exon
Length: 76
Transcript: RAD52.cAug05
Exon
Length: 102
Transcript: RAD52.cAug05
Exon
Length: 102
Transcript: RAD52.cAug05
Exon
Length: 94
Transcript: RAD52.cAug05
Exon
Length: 123
Transcript: RAD52.cAug05
Exon
Length: 119
Transcript: RAD52.cAug05
Exon
Length: 151
Transcript: RAD52.cAug05
Exon
Length: 76
Transcript: RAD52.cAug05
Exon
Length: 182
Transcript: RAD52.cAug05
Exon
Length: 140
Transcript: RAD52.cAug05
Exon
Length: 102
Transcript: RAD52.cAug05
Exon
Length: 228
Transcript: RAD52.cAug05
Exon
Length: 962
Transcript: RAD52.cAug05
Intron
Length: 12719
Transcript: RAD52.cAug05
Intron
Length: 3730
Transcript: RAD52.cAug05
Intron
Length: 1653
Transcript: RAD52.cAug05
Intron
Length: 1075
Transcript: RAD52.cAug05
Intron
Length: 164
Transcript: RAD52.cAug05
Intron
Length: 2500
Transcript: RAD52.cAug05
Intron
Length: 198
Transcript: RAD52.cAug05
Intron
Length: 1270
Transcript: RAD52.cAug05
Intron
Length: 8629
Transcript: RAD52.cAug05
Intron
Length: 155
Transcript: RAD52.cAug05
Intron
Length: 1811
Transcript: RAD52.cAug05
Intron
Length: 309
Transcript: RAD52.cAug05
Intron
Length: 441
Transcript: RAD52.cAug05
Reaction
Expected peak: 387
Associated peak: 419,420,421,422,423,424,437,440
Repeated 51 time
Molarity: 1.28M
Forward: RAD52.u.f.18
Reverse: RAD52.u.r.18
Reaction
Expected peak: 536
Associated peak: 483,488,490,491,493,495,500,517
Repeated 51 time
Molarity: 0.51M
Forward: RAD52.u.f.15
Reverse: RAD52.u.r.18
Exon
Length: 116
Transcript: RAD52.dAug05
Exon
Length: 102
Transcript: RAD52.dAug05
Exon
Length: 102
Transcript: RAD52.dAug05
Exon
Length: 94
Transcript: RAD52.dAug05
Exon
Length: 68
Transcript: RAD52.dAug05
Exon
Length: 119
Transcript: RAD52.dAug05
Exon
Length: 76
Transcript: RAD52.dAug05
Exon
Length: 188
Transcript: RAD52.dAug05
Intron
Length: 16525
Transcript: RAD52.dAug05
Intron
Length: 1653
Transcript: RAD52.dAug05
Intron
Length: 1075
Transcript: RAD52.dAug05
Intron
Length: 164
Transcript: RAD52.dAug05
Intron
Length: 2555
Transcript: RAD52.dAug05
Intron
Length: 1619
Transcript: RAD52.dAug05
Intron
Length: 8629
Transcript: RAD52.dAug05
Reaction
Expected peak: 236
Associated peak: 245,246,247,248,249,254,255,256
Repeated 51 time
Molarity: 24.76M
Forward: RAD52.u.f.18
Reverse: RAD52.u.r.18
Reaction
Expected peak: 330
Associated peak: 341,342,343,344,345,346,347,348,349,350,351,352
Repeated 51 time
Molarity: 16.10M
Forward: RAD52.u.f.15
Reverse: RAD52.u.r.18
Exon
Length: 81
Transcript: RAD52.eAug05
Exon
Length: 102
Transcript: RAD52.eAug05
Exon
Length: 102
Transcript: RAD52.eAug05
Exon
Length: 94
Transcript: RAD52.eAug05
Exon
Length: 123
Transcript: RAD52.eAug05
Exon
Length: 468
Transcript: RAD52.eAug05
Exon
Length: 76
Transcript: RAD52.eAug05
Exon
Length: 182
Transcript: RAD52.eAug05
Exon
Length: 140
Transcript: RAD52.eAug05
Exon
Length: 122
Transcript: RAD52.eAug05
Exon
Length: 228
Transcript: RAD52.eAug05
Exon
Length: 1376
Transcript: RAD52.eAug05
Intron
Length: 16525
Transcript: RAD52.eAug05
Intron
Length: 1653
Transcript: RAD52.eAug05
Intron
Length: 1075
Transcript: RAD52.eAug05
Intron
Length: 164
Transcript: RAD52.eAug05
Intron
Length: 2500
Transcript: RAD52.eAug05
Intron
Length: 1270
Transcript: RAD52.eAug05
Intron
Length: 8629
Transcript: RAD52.eAug05
Intron
Length: 155
Transcript: RAD52.eAug05
Intron
Length: 1811
Transcript: RAD52.eAug05
Intron
Length: 289
Transcript: RAD52.eAug05
Intron
Length: 441
Transcript: RAD52.eAug05
Reaction
Expected peak: 585
Associated peak: 583,586,589,597,609,612,614,618,620,624,642
Repeated 51 time
Molarity: 0.28M
Forward: RAD52.u.f.18
Reverse: RAD52.u.r.18
Reaction
Expected peak: 734
Associated peak: None
Repeated 51 time
Molarity: 0.00M
Forward: RAD52.u.f.15
Reverse: RAD52.u.r.18
Exon
Length: 81
Transcript: RAD52.fAug05
Exon
Length: 102
Transcript: RAD52.fAug05
Exon
Length: 102
Transcript: RAD52.fAug05
Exon
Length: 94
Transcript: RAD52.fAug05
Exon
Length: 68
Transcript: RAD52.fAug05
Exon
Length: 119
Transcript: RAD52.fAug05
Exon
Length: 151
Transcript: RAD52.fAug05
Exon
Length: 76
Transcript: RAD52.fAug05
Exon
Length: 182
Transcript: RAD52.fAug05
Exon
Length: 140
Transcript: RAD52.fAug05
Exon
Length: 122
Transcript: RAD52.fAug05
Exon
Length: 228
Transcript: RAD52.fAug05
Exon
Length: 1376
Transcript: RAD52.fAug05
Intron
Length: 16525
Transcript: RAD52.fAug05
Intron
Length: 1653
Transcript: RAD52.fAug05
Intron
Length: 1075
Transcript: RAD52.fAug05
Intron
Length: 164
Transcript: RAD52.fAug05
Intron
Length: 2555
Transcript: RAD52.fAug05
Intron
Length: 198
Transcript: RAD52.fAug05
Intron
Length: 1270
Transcript: RAD52.fAug05
Intron
Length: 8629
Transcript: RAD52.fAug05
Intron
Length: 155
Transcript: RAD52.fAug05
Intron
Length: 1811
Transcript: RAD52.fAug05
Intron
Length: 289
Transcript: RAD52.fAug05
Intron
Length: 441
Transcript: RAD52.fAug05
Reaction
Expected peak: 387
Associated peak: 419,420,421,422,423,424,437,440
Repeated 51 time
Molarity: 1.28M
Forward: RAD52.u.f.18
Reverse: RAD52.u.r.18
Reaction
Expected peak: 481
Associated peak: 476,477,478,483,488,490,491,493,495,500
Repeated 51 time
Molarity: 0.64M
Forward: RAD52.u.f.15
Reverse: RAD52.u.r.18
Exon
Length: 289
Transcript: RAD52.gAug05
Exon
Length: 102
Transcript: RAD52.gAug05
Exon
Length: 228
Transcript: RAD52.gAug05
Exon
Length: 202
Transcript: RAD52.gAug05
Intron
Length: 1811
Transcript: RAD52.gAug05
Intron
Length: 309
Transcript: RAD52.gAug05
Intron
Length: 441
Transcript: RAD52.gAug05
Exon
Length: 62
Transcript: RAD52.hAug05
Exon
Length: 102
Transcript: RAD52.hAug05
Exon
Length: 102
Transcript: RAD52.hAug05
Exon
Length: 94
Transcript: RAD52.hAug05
Exon
Length: 68
Transcript: RAD52.hAug05
Exon
Length: 119
Transcript: RAD52.hAug05
Exon
Length: 35
Transcript: RAD52.hAug05
Intron
Length: 16529
Transcript: RAD52.hAug05
Intron
Length: 1653
Transcript: RAD52.hAug05
Intron
Length: 1075
Transcript: RAD52.hAug05
Intron
Length: 164
Transcript: RAD52.hAug05
Intron
Length: 2555
Transcript: RAD52.hAug05
Intron
Length: 1619
Transcript: RAD52.hAug05
Exon
Length: 81
Transcript: RAD52.iAug05
Exon
Length: 102
Transcript: RAD52.iAug05
Exon
Length: 102
Transcript: RAD52.iAug05
Exon
Length: 381
Transcript: RAD52.iAug05
Exon
Length: 119
Transcript: RAD52.iAug05
Exon
Length: 76
Transcript: RAD52.iAug05
Exon
Length: 182
Transcript: RAD52.iAug05
Exon
Length: 189
Transcript: RAD52.iAug05
Exon
Length: 102
Transcript: RAD52.iAug05
Exon
Length: 228
Transcript: RAD52.iAug05
Exon
Length: 1376
Transcript: RAD52.iAug05
Intron
Length: 16525
Transcript: RAD52.iAug05
Intron
Length: 1653
Transcript: RAD52.iAug05
Intron
Length: 1075
Transcript: RAD52.iAug05
Intron
Length: 2500
Transcript: RAD52.iAug05
Intron
Length: 1619
Transcript: RAD52.iAug05
Intron
Length: 8629
Transcript: RAD52.iAug05
Intron
Length: 106
Transcript: RAD52.iAug05
Intron
Length: 1811
Transcript: RAD52.iAug05
Intron
Length: 309
Transcript: RAD52.iAug05
Intron
Length: 441
Transcript: RAD52.iAug05
Reaction
Expected peak: 236
Associated peak: 245,246,247,248,249,254,255,256
Repeated 51 time
Molarity: 24.76M
Forward: RAD52.u.f.18
Reverse: RAD52.u.r.18
Reaction
Expected peak: 385
Associated peak: 366,410,413,414,415,416,418,419,420
Repeated 51 time
Molarity: 0.51M
Forward: RAD52.u.f.15
Reverse: RAD52.u.r.18
Exon
Length: 239
Transcript: RAD52.jAug05
Exon
Length: 122
Transcript: RAD52.jAug05
Exon
Length: 228
Transcript: RAD52.jAug05
Exon
Length: 219
Transcript: RAD52.jAug05
Intron
Length: 1811
Transcript: RAD52.jAug05
Intron
Length: 289
Transcript: RAD52.jAug05
Intron
Length: 441
Transcript: RAD52.jAug05
Exon
Length: 349
Transcript: RAD52.kAug05
Exon
Length: 94
Transcript: RAD52.kAug05
Intron
Length: 441
Transcript: RAD52.kAug05
Exon
Length: 117
Transcript: RAD52.lAug05
Exon
Length: 102
Transcript: RAD52.lAug05
Exon
Length: 94
Transcript: RAD52.lAug05
Exon
Length: 98
Transcript: RAD52.lAug05
Intron
Length: 18081
Transcript: RAD52.lAug05
Intron
Length: 1075
Transcript: RAD52.lAug05
Intron
Length: 164
Transcript: RAD52.lAug05
Exon
Length: 17
Transcript: RAD52.mAug05
Exon
Length: 102
Transcript: RAD52.mAug05
Exon
Length: 94
Transcript: RAD52.mAug05
Exon
Length: 123
Transcript: RAD52.mAug05
Exon
Length: 119
Transcript: RAD52.mAug05
Exon
Length: 151
Transcript: RAD52.mAug05
Exon
Length: 76
Transcript: RAD52.mAug05
Exon
Length: 183
Transcript: RAD52.mAug05
Intron
Length: 1653
Transcript: RAD52.mAug05
Intron
Length: 1075
Transcript: RAD52.mAug05
Intron
Length: 164
Transcript: RAD52.mAug05
Intron
Length: 2500
Transcript: RAD52.mAug05
Intron
Length: 198
Transcript: RAD52.mAug05
Intron
Length: 1270
Transcript: RAD52.mAug05
Intron
Length: 8629
Transcript: RAD52.mAug05
Reaction
Expected peak: 387
Associated peak: 419,420,421,422,423,424,437,440
Repeated 51 time
Molarity: 1.28M
Forward: RAD52.u.f.18
Reverse: RAD52.u.r.18
Reaction
Expected peak: 536
Associated peak: 483,488,490,491,493,495,500,517
Repeated 51 time
Molarity: 0.51M
Forward: RAD52.u.f.15
Reverse: RAD52.u.r.18
Exon
Length: 43
Transcript: RAD52.nAug05
Exon
Length: 233
Transcript: RAD52.nAug05
Exon
Length: 2077
Transcript: RAD52.nAug05
Intron
Length: 1009
Transcript: RAD52.nAug05
Intron
Length: 12702
Transcript: RAD52.nAug05
Exon
Length: 574
Transcript: RAD52.shed1.aAug05
Exon
Length: 753
Transcript: RAD52.shed2.aAug05
RAD52.u.r.18
TCGGCAGCTGTTGTATCTTG
RAD52.u.f.15
AATGGCAAGTTCTACGTGGG
RAD52.u.f.18
GCCTCAAGTCCAAGGCTTTAT

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