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2. Breast Validation Screen 
Gene primer pair list : Primer pairs
Forward Reverse Occurences View
VDR.F2 VDR.R2 51 View
VDR.F3 VDR.R3 51 View
Transcript map : VDR
Exon
Length: 440
Transcript: VDR.aAug05
Exon
Length: 81
Transcript: VDR.aAug05
Exon
Length: 148
Transcript: VDR.aAug05
Exon
Length: 131
Transcript: VDR.aAug05
Exon
Length: 185
Transcript: VDR.aAug05
Exon
Length: 121
Transcript: VDR.aAug05
Exon
Length: 172
Transcript: VDR.aAug05
Exon
Length: 152
Transcript: VDR.aAug05
Exon
Length: 117
Transcript: VDR.aAug05
Exon
Length: 2362
Transcript: VDR.aAug05
Intron
Length: 21789
Transcript: VDR.aAug05
Intron
Length: 3578
Transcript: VDR.aAug05
Intron
Length: 13790
Transcript: VDR.aAug05
Intron
Length: 7358
Transcript: VDR.aAug05
Intron
Length: 254
Transcript: VDR.aAug05
Intron
Length: 1327
Transcript: VDR.aAug05
Intron
Length: 8821
Transcript: VDR.aAug05
Intron
Length: 205
Transcript: VDR.aAug05
Intron
Length: 1329
Transcript: VDR.aAug05
Reaction
Expected peak: 493
Associated peak: 481,483,484,494,497,513
Repeated 51 time
Molarity: 0.41M
Forward: VDR.F2
Reverse: VDR.R2
Exon
Length: 77
Transcript: VDR.bAug05
Exon
Length: 122
Transcript: VDR.bAug05
Exon
Length: 81
Transcript: VDR.bAug05
Exon
Length: 148
Transcript: VDR.bAug05
Exon
Length: 131
Transcript: VDR.bAug05
Exon
Length: 185
Transcript: VDR.bAug05
Exon
Length: 121
Transcript: VDR.bAug05
Exon
Length: 172
Transcript: VDR.bAug05
Exon
Length: 152
Transcript: VDR.bAug05
Exon
Length: 117
Transcript: VDR.bAug05
Exon
Length: 3467
Transcript: VDR.bAug05
Intron
Length: 4996
Transcript: VDR.bAug05
Intron
Length: 17062
Transcript: VDR.bAug05
Intron
Length: 3578
Transcript: VDR.bAug05
Intron
Length: 13790
Transcript: VDR.bAug05
Intron
Length: 7358
Transcript: VDR.bAug05
Intron
Length: 254
Transcript: VDR.bAug05
Intron
Length: 1327
Transcript: VDR.bAug05
Intron
Length: 8821
Transcript: VDR.bAug05
Intron
Length: 205
Transcript: VDR.bAug05
Intron
Length: 1329
Transcript: VDR.bAug05
Reaction
Expected peak: 224
Associated peak: 238,239,240,241,248,249,250
Repeated 51 time
Molarity: 2.40M
Forward: VDR.F2
Reverse: VDR.R2
Reaction
Expected peak: 273
Associated peak: 282,283,284,285,286
Repeated 51 time
Molarity: 9.65M
Forward: VDR.F3
Reverse: VDR.R3
Exon
Length: 77
Transcript: VDR.cAug05
Exon
Length: 81
Transcript: VDR.cAug05
Exon
Length: 148
Transcript: VDR.cAug05
Exon
Length: 131
Transcript: VDR.cAug05
Exon
Length: 185
Transcript: VDR.cAug05
Exon
Length: 121
Transcript: VDR.cAug05
Exon
Length: 172
Transcript: VDR.cAug05
Exon
Length: 152
Transcript: VDR.cAug05
Exon
Length: 117
Transcript: VDR.cAug05
Exon
Length: 3469
Transcript: VDR.cAug05
Intron
Length: 22180
Transcript: VDR.cAug05
Intron
Length: 3578
Transcript: VDR.cAug05
Intron
Length: 13790
Transcript: VDR.cAug05
Intron
Length: 7358
Transcript: VDR.cAug05
Intron
Length: 254
Transcript: VDR.cAug05
Intron
Length: 1327
Transcript: VDR.cAug05
Intron
Length: 8821
Transcript: VDR.cAug05
Intron
Length: 205
Transcript: VDR.cAug05
Intron
Length: 1329
Transcript: VDR.cAug05
Reaction
Expected peak: 102
Associated peak: 108,109,110,111
Repeated 51 time
Molarity: 27.69M
Forward: VDR.F2
Reverse: VDR.R2
Exon
Length: 62
Transcript: VDR.dAug05
Exon
Length: 122
Transcript: VDR.dAug05
Exon
Length: 148
Transcript: VDR.dAug05
Exon
Length: 131
Transcript: VDR.dAug05
Exon
Length: 185
Transcript: VDR.dAug05
Exon
Length: 121
Transcript: VDR.dAug05
Exon
Length: 168
Transcript: VDR.dAug05
Intron
Length: 4996
Transcript: VDR.dAug05
Intron
Length: 20721
Transcript: VDR.dAug05
Intron
Length: 13790
Transcript: VDR.dAug05
Intron
Length: 7358
Transcript: VDR.dAug05
Intron
Length: 254
Transcript: VDR.dAug05
Intron
Length: 1327
Transcript: VDR.dAug05
Reaction
Expected peak: 192
Associated peak: 204,205,206,207,208,215
Repeated 51 time
Molarity: 2.58M
Forward: VDR.F3
Reverse: VDR.R3
VDR.F2
AGCGCGGAACAGCTTGTC
VDR.R3
GCATTGAAGTGAAAGCCAGTG
VDR.F3
TCCTCTGATAGCCTCATGCC
VDR.R2
CAGGTAAGTGGAGCCCAGG

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