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2. Breast Validation Screen 
Gene primer pair list : Primer pairs
Forward Reverse Occurences View
SHMT1.u.f.24 SHMT1.u.r.18 51 View
Transcript map : SHMT1
Exon
Length: 183
Transcript: SHMT1.aAug05
Exon
Length: 115
Transcript: SHMT1.aAug05
Exon
Length: 146
Transcript: SHMT1.aAug05
Exon
Length: 116
Transcript: SHMT1.aAug05
Exon
Length: 161
Transcript: SHMT1.aAug05
Exon
Length: 82
Transcript: SHMT1.aAug05
Exon
Length: 213
Transcript: SHMT1.aAug05
Exon
Length: 117
Transcript: SHMT1.aAug05
Exon
Length: 123
Transcript: SHMT1.aAug05
Exon
Length: 117
Transcript: SHMT1.aAug05
Exon
Length: 111
Transcript: SHMT1.aAug05
Exon
Length: 4835
Transcript: SHMT1.aAug05
Intron
Length: 7371
Transcript: SHMT1.aAug05
Intron
Length: 2068
Transcript: SHMT1.aAug05
Intron
Length: 5231
Transcript: SHMT1.aAug05
Intron
Length: 668
Transcript: SHMT1.aAug05
Intron
Length: 6682
Transcript: SHMT1.aAug05
Intron
Length: 476
Transcript: SHMT1.aAug05
Intron
Length: 4367
Transcript: SHMT1.aAug05
Intron
Length: 2270
Transcript: SHMT1.aAug05
Intron
Length: 2494
Transcript: SHMT1.aAug05
Intron
Length: 1166
Transcript: SHMT1.aAug05
Intron
Length: 358
Transcript: SHMT1.aAug05
Reaction
Expected peak: 225
Associated peak: 232,233,234,235,236,237,238,239,240
Repeated 51 time
Molarity: 27.98M
Forward: SHMT1.u.f.24
Reverse: SHMT1.u.r.18
Exon
Length: 35
Transcript: SHMT1.bAug05
Exon
Length: 139
Transcript: SHMT1.bAug05
Exon
Length: 115
Transcript: SHMT1.bAug05
Exon
Length: 146
Transcript: SHMT1.bAug05
Exon
Length: 116
Transcript: SHMT1.bAug05
Exon
Length: 161
Transcript: SHMT1.bAug05
Exon
Length: 82
Transcript: SHMT1.bAug05
Exon
Length: 213
Transcript: SHMT1.bAug05
Exon
Length: 117
Transcript: SHMT1.bAug05
Exon
Length: 123
Transcript: SHMT1.bAug05
Exon
Length: 117
Transcript: SHMT1.bAug05
Exon
Length: 85
Transcript: SHMT1.bAug05
Exon
Length: 339
Transcript: SHMT1.bAug05
Intron
Length: 2819
Transcript: SHMT1.bAug05
Intron
Length: 4413
Transcript: SHMT1.bAug05
Intron
Length: 2068
Transcript: SHMT1.bAug05
Intron
Length: 5231
Transcript: SHMT1.bAug05
Intron
Length: 668
Transcript: SHMT1.bAug05
Intron
Length: 6682
Transcript: SHMT1.bAug05
Intron
Length: 476
Transcript: SHMT1.bAug05
Intron
Length: 4367
Transcript: SHMT1.bAug05
Intron
Length: 2270
Transcript: SHMT1.bAug05
Intron
Length: 2494
Transcript: SHMT1.bAug05
Intron
Length: 1166
Transcript: SHMT1.bAug05
Intron
Length: 412
Transcript: SHMT1.bAug05
Reaction
Expected peak: 225
Associated peak: 232,233,234,235,236,237,238,239,240
Repeated 51 time
Molarity: 27.98M
Forward: SHMT1.u.f.24
Reverse: SHMT1.u.r.18
Exon
Length: 182
Transcript: SHMT1.cAug05
Exon
Length: 115
Transcript: SHMT1.cAug05
Exon
Length: 146
Transcript: SHMT1.cAug05
Exon
Length: 116
Transcript: SHMT1.cAug05
Exon
Length: 161
Transcript: SHMT1.cAug05
Exon
Length: 82
Transcript: SHMT1.cAug05
Exon
Length: 213
Transcript: SHMT1.cAug05
Exon
Length: 117
Transcript: SHMT1.cAug05
Exon
Length: 123
Transcript: SHMT1.cAug05
Exon
Length: 117
Transcript: SHMT1.cAug05
Exon
Length: 396
Transcript: SHMT1.cAug05
Intron
Length: 7371
Transcript: SHMT1.cAug05
Intron
Length: 2068
Transcript: SHMT1.cAug05
Intron
Length: 5231
Transcript: SHMT1.cAug05
Intron
Length: 668
Transcript: SHMT1.cAug05
Intron
Length: 6682
Transcript: SHMT1.cAug05
Intron
Length: 476
Transcript: SHMT1.cAug05
Intron
Length: 4367
Transcript: SHMT1.cAug05
Intron
Length: 2270
Transcript: SHMT1.cAug05
Intron
Length: 2494
Transcript: SHMT1.cAug05
Intron
Length: 1635
Transcript: SHMT1.cAug05
Reaction
Expected peak: 225
Associated peak: 232,233,234,235,236,237,238,239,240
Repeated 51 time
Molarity: 27.98M
Forward: SHMT1.u.f.24
Reverse: SHMT1.u.r.18
Exon
Length: 171
Transcript: SHMT1.dAug05
Exon
Length: 115
Transcript: SHMT1.dAug05
Exon
Length: 146
Transcript: SHMT1.dAug05
Exon
Length: 116
Transcript: SHMT1.dAug05
Exon
Length: 161
Transcript: SHMT1.dAug05
Exon
Length: 82
Transcript: SHMT1.dAug05
Exon
Length: 213
Transcript: SHMT1.dAug05
Exon
Length: 123
Transcript: SHMT1.dAug05
Exon
Length: 117
Transcript: SHMT1.dAug05
Exon
Length: 111
Transcript: SHMT1.dAug05
Exon
Length: 1046
Transcript: SHMT1.dAug05
Intron
Length: 7371
Transcript: SHMT1.dAug05
Intron
Length: 2068
Transcript: SHMT1.dAug05
Intron
Length: 5231
Transcript: SHMT1.dAug05
Intron
Length: 668
Transcript: SHMT1.dAug05
Intron
Length: 6682
Transcript: SHMT1.dAug05
Intron
Length: 476
Transcript: SHMT1.dAug05
Intron
Length: 6754
Transcript: SHMT1.dAug05
Intron
Length: 2494
Transcript: SHMT1.dAug05
Intron
Length: 1166
Transcript: SHMT1.dAug05
Intron
Length: 358
Transcript: SHMT1.dAug05
Reaction
Expected peak: 108
Associated peak: 110,111,112,113,114,115,116,118,119,120
Repeated 51 time
Molarity: 24.28M
Forward: SHMT1.u.f.24
Reverse: SHMT1.u.r.18
Exon
Length: 924
Transcript: SHMT1.eAug05
Exon
Length: 213
Transcript: SHMT1.eAug05
Exon
Length: 117
Transcript: SHMT1.eAug05
Exon
Length: 123
Transcript: SHMT1.eAug05
Exon
Length: 117
Transcript: SHMT1.eAug05
Exon
Length: 111
Transcript: SHMT1.eAug05
Exon
Length: 1046
Transcript: SHMT1.eAug05
Intron
Length: 476
Transcript: SHMT1.eAug05
Intron
Length: 4367
Transcript: SHMT1.eAug05
Intron
Length: 2270
Transcript: SHMT1.eAug05
Intron
Length: 2494
Transcript: SHMT1.eAug05
Intron
Length: 1166
Transcript: SHMT1.eAug05
Intron
Length: 358
Transcript: SHMT1.eAug05
Reaction
Expected peak: 225
Associated peak: 232,233,234,235,236,237,238,239,240
Repeated 51 time
Molarity: 27.98M
Forward: SHMT1.u.f.24
Reverse: SHMT1.u.r.18
Exon
Length: 15
Transcript: SHMT1.fAug05
Exon
Length: 139
Transcript: SHMT1.fAug05
Exon
Length: 111
Transcript: SHMT1.fAug05
Exon
Length: 146
Transcript: SHMT1.fAug05
Exon
Length: 116
Transcript: SHMT1.fAug05
Exon
Length: 161
Transcript: SHMT1.fAug05
Exon
Length: 82
Transcript: SHMT1.fAug05
Exon
Length: 174
Transcript: SHMT1.fAug05
Intron
Length: 2819
Transcript: SHMT1.fAug05
Intron
Length: 4417
Transcript: SHMT1.fAug05
Intron
Length: 2068
Transcript: SHMT1.fAug05
Intron
Length: 5231
Transcript: SHMT1.fAug05
Intron
Length: 668
Transcript: SHMT1.fAug05
Intron
Length: 6682
Transcript: SHMT1.fAug05
Intron
Length: 476
Transcript: SHMT1.fAug05
Exon
Length: 83
Transcript: SHMT1.gAug05
Exon
Length: 115
Transcript: SHMT1.gAug05
Exon
Length: 146
Transcript: SHMT1.gAug05
Exon
Length: 67
Transcript: SHMT1.gAug05
Exon
Length: 152
Transcript: SHMT1.gAug05
Exon
Length: 82
Transcript: SHMT1.gAug05
Intron
Length: 7399
Transcript: SHMT1.gAug05
Intron
Length: 2068
Transcript: SHMT1.gAug05
Intron
Length: 5231
Transcript: SHMT1.gAug05
Intron
Length: 726
Transcript: SHMT1.gAug05
Intron
Length: 6682
Transcript: SHMT1.gAug05
Exon
Length: 334
Transcript: SHMT1.hAug05
Exon
Length: 115
Transcript: SHMT1.hAug05
Exon
Length: 146
Transcript: SHMT1.hAug05
Exon
Length: 116
Transcript: SHMT1.hAug05
Exon
Length: 98
Transcript: SHMT1.hAug05
Intron
Length: 6987
Transcript: SHMT1.hAug05
Intron
Length: 2068
Transcript: SHMT1.hAug05
Intron
Length: 5231
Transcript: SHMT1.hAug05
Intron
Length: 668
Transcript: SHMT1.hAug05
Exon
Length: 147
Transcript: SHMT1.iAug05
Exon
Length: 111
Transcript: SHMT1.iAug05
Exon
Length: 146
Transcript: SHMT1.iAug05
Exon
Length: 116
Transcript: SHMT1.iAug05
Exon
Length: 152
Transcript: SHMT1.iAug05
Intron
Length: 7375
Transcript: SHMT1.iAug05
Intron
Length: 2068
Transcript: SHMT1.iAug05
Intron
Length: 5231
Transcript: SHMT1.iAug05
Intron
Length: 668
Transcript: SHMT1.iAug05
Exon
Length: 171
Transcript: SHMT1.jAug05
Exon
Length: 139
Transcript: SHMT1.jAug05
Exon
Length: 115
Transcript: SHMT1.jAug05
Exon
Length: 146
Transcript: SHMT1.jAug05
Exon
Length: 153
Transcript: SHMT1.jAug05
Intron
Length: 2819
Transcript: SHMT1.jAug05
Intron
Length: 4413
Transcript: SHMT1.jAug05
Intron
Length: 2068
Transcript: SHMT1.jAug05
Intron
Length: 6015
Transcript: SHMT1.jAug05
Exon
Length: 192
Transcript: SHMT1.kAug05
Exon
Length: 115
Transcript: SHMT1.kAug05
Exon
Length: 146
Transcript: SHMT1.kAug05
Exon
Length: 82
Transcript: SHMT1.kAug05
Exon
Length: 12
Transcript: SHMT1.kAug05
Exon
Length: 161
Transcript: SHMT1.kAug05
Exon
Length: 82
Transcript: SHMT1.kAug05
Intron
Length: 7371
Transcript: SHMT1.kAug05
Intron
Length: 2068
Transcript: SHMT1.kAug05
Intron
Length: 4279
Transcript: SHMT1.kAug05
Intron
Length: 443
Transcript: SHMT1.kAug05
Intron
Length: 1199
Transcript: SHMT1.kAug05
Intron
Length: 6682
Transcript: SHMT1.kAug05
Exon
Length: 13
Transcript: SHMT1.lAug05
Exon
Length: 117
Transcript: SHMT1.lAug05
Exon
Length: 111
Transcript: SHMT1.lAug05
Exon
Length: 109
Transcript: SHMT1.lAug05
Intron
Length: 9371
Transcript: SHMT1.lAug05
Intron
Length: 1166
Transcript: SHMT1.lAug05
Intron
Length: 358
Transcript: SHMT1.lAug05
Exon
Length: 93
Transcript: SHMT1.mAug05
Exon
Length: 406
Transcript: SHMT1.mAug05
Exon
Length: 344
Transcript: SHMT1.mAug05
Intron
Length: 386
Transcript: SHMT1.mAug05
Intron
Length: 54
Transcript: SHMT1.mAug05
Exon
Length: 683
Transcript: SHMT1.shed1.aAug05
Exon
Length: 843
Transcript: SHMT1.shed2.aAug05
SHMT1.u.r.18
CATAGCTTGCTTCAGTGCCA
SHMT1.u.f.24
GAACACTGCCATGTGGTGAC

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