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2. Breast Validation Screen 
Transcript map : KLK8
Exon
Length: 171
Transcript: KLK8.aAug05
Exon
Length: 78
Transcript: KLK8.aAug05
Exon
Length: 295
Transcript: KLK8.aAug05
Exon
Length: 263
Transcript: KLK8.aAug05
Exon
Length: 134
Transcript: KLK8.aAug05
Exon
Length: 207
Transcript: KLK8.aAug05
Intron
Length: 356
Transcript: KLK8.aAug05
Intron
Length: 379
Transcript: KLK8.aAug05
Intron
Length: 165
Transcript: KLK8.aAug05
Intron
Length: 2111
Transcript: KLK8.aAug05
Intron
Length: 1536
Transcript: KLK8.aAug05
Reaction
Expected peak: 305
Associated peak: 290,317,318,319,320,321,322,323
Repeated 51 time
Molarity: 1.05M
Forward: KLK8.F1
Reverse: KLK8.R13
Reaction
Expected peak: 475
Associated peak: 500,505,506,511,512,517
Repeated 51 time
Molarity: 1.01M
Forward: KLK8.F1
Reverse: KLK8.R1
Reaction
Expected peak: 792
Associated peak: 847,856
Repeated 51 time
Molarity: 0.06M
Forward: KLK8.F1
Reverse: KLK8.R7
Reaction
Expected peak: 844
Associated peak: 763,765,767,778,889
Repeated 51 time
Molarity: 0.12M
Forward: KLK8.F1
Reverse: KLK8.R3
Exon
Length: 130
Transcript: KLK8.bAug05
Exon
Length: 157
Transcript: KLK8.bAug05
Exon
Length: 266
Transcript: KLK8.bAug05
Exon
Length: 137
Transcript: KLK8.bAug05
Exon
Length: 853
Transcript: KLK8.bAug05
Exon
Length: 178
Transcript: KLK8.bAug05
Exon
Length: 78
Transcript: KLK8.bAug05
Exon
Length: 160
Transcript: KLK8.bAug05
Exon
Length: 263
Transcript: KLK8.bAug05
Exon
Length: 134
Transcript: KLK8.bAug05
Exon
Length: 207
Transcript: KLK8.bAug05
Intron
Length: 165
Transcript: KLK8.bAug05
Intron
Length: 2459
Transcript: KLK8.bAug05
Intron
Length: 2617
Transcript: KLK8.bAug05
Intron
Length: 443
Transcript: KLK8.bAug05
Intron
Length: 698
Transcript: KLK8.bAug05
Intron
Length: 356
Transcript: KLK8.bAug05
Intron
Length: 514
Transcript: KLK8.bAug05
Intron
Length: 165
Transcript: KLK8.bAug05
Intron
Length: 2111
Transcript: KLK8.bAug05
Intron
Length: 1536
Transcript: KLK8.bAug05
Reaction
Expected peak: 170
Associated peak: 185,186,187,188,189
Repeated 51 time
Molarity: 4.67M
Forward: KLK8.F1
Reverse: KLK8.R13
Reaction
Expected peak: 340
Associated peak: 373,374,375,376,377,378,379,380
Repeated 51 time
Molarity: 3.96M
Forward: KLK8.F1
Reverse: KLK8.R1
Reaction
Expected peak: 657
Associated peak: 689,694,695,700,711,722
Repeated 51 time
Molarity: 0.56M
Forward: KLK8.F1
Reverse: KLK8.R7
Reaction
Expected peak: 709
Associated peak: 688,741,751,754,756,763,765,767,778
Repeated 51 time
Molarity: 0.20M
Forward: KLK8.F1
Reverse: KLK8.R3
Exon
Length: 130
Transcript: KLK8.cAug05
Exon
Length: 266
Transcript: KLK8.cAug05
Exon
Length: 137
Transcript: KLK8.cAug05
Exon
Length: 853
Transcript: KLK8.cAug05
Exon
Length: 78
Transcript: KLK8.cAug05
Exon
Length: 263
Transcript: KLK8.cAug05
Exon
Length: 134
Transcript: KLK8.cAug05
Exon
Length: 327
Transcript: KLK8.cAug05
Intron
Length: 2781
Transcript: KLK8.cAug05
Intron
Length: 2617
Transcript: KLK8.cAug05
Intron
Length: 443
Transcript: KLK8.cAug05
Intron
Length: 1232
Transcript: KLK8.cAug05
Intron
Length: 839
Transcript: KLK8.cAug05
Intron
Length: 2111
Transcript: KLK8.cAug05
Intron
Length: 1536
Transcript: KLK8.cAug05
Reaction
Expected peak: 180
Associated peak: 199,200,201
Repeated 51 time
Molarity: 1.08M
Forward: KLK8.F1
Reverse: KLK8.R1
Reaction
Expected peak: 497
Associated peak: 450,500,522,524,527,529,533,535
Repeated 51 time
Molarity: 0.18M
Forward: KLK8.F1
Reverse: KLK8.R7
Reaction
Expected peak: 549
Associated peak: 500,506,565,594,597,600
Repeated 51 time
Molarity: 0.12M
Forward: KLK8.F1
Reverse: KLK8.R3
Exon
Length: 11
Transcript: KLK8.dAug05
Exon
Length: 160
Transcript: KLK8.dAug05
Exon
Length: 263
Transcript: KLK8.dAug05
Exon
Length: 134
Transcript: KLK8.dAug05
Exon
Length: 95
Transcript: KLK8.dAug05
Exon
Length: 86
Transcript: KLK8.dAug05
Intron
Length: 514
Transcript: KLK8.dAug05
Intron
Length: 165
Transcript: KLK8.dAug05
Intron
Length: 2111
Transcript: KLK8.dAug05
Intron
Length: 1182
Transcript: KLK8.dAug05
Intron
Length: 259
Transcript: KLK8.dAug05
Reaction
Expected peak: 170
Associated peak: 185,186,187,188,189
Repeated 51 time
Molarity: 4.67M
Forward: KLK8.F1
Reverse: KLK8.R13
Reaction
Expected peak: 340
Associated peak: 373,374,375,376,377,378,379,380
Repeated 51 time
Molarity: 3.96M
Forward: KLK8.F1
Reverse: KLK8.R1
Reaction
Expected peak: 752
Associated peak: 689,694,695,700,711,722
Repeated 51 time
Molarity: 0.56M
Forward: KLK8.F1
Reverse: KLK8.R7
Reaction
Expected peak: 804
Associated peak: 741,751,754,756,763,765,767,778
Repeated 51 time
Molarity: 0.20M
Forward: KLK8.F1
Reverse: KLK8.R3
Exon
Length: 171
Transcript: KLK8.eAug05
Exon
Length: 78
Transcript: KLK8.eAug05
Exon
Length: 134
Transcript: KLK8.eAug05
Exon
Length: 207
Transcript: KLK8.eAug05
Intron
Length: 356
Transcript: KLK8.eAug05
Intron
Length: 3213
Transcript: KLK8.eAug05
Intron
Length: 1536
Transcript: KLK8.eAug05
Reaction
Expected peak: 234
Associated peak: 249,250,251,252,253
Repeated 51 time
Molarity: 7.14M
Forward: KLK8.F1
Reverse: KLK8.R7
Reaction
Expected peak: 286
Associated peak: 295,296,297,298,299
Repeated 51 time
Molarity: 7.63M
Forward: KLK8.F1
Reverse: KLK8.R3
Exon
Length: 217
Transcript: KLK8.fAug05
Exon
Length: 206
Transcript: KLK8.fAug05
Intron
Length: 1536
Transcript: KLK8.fAug05
Exon
Length: 171
Transcript: KLK8.gAug05
Exon
Length: 78
Transcript: KLK8.gAug05
Exon
Length: 207
Transcript: KLK8.gAug05
Intron
Length: 356
Transcript: KLK8.gAug05
Intron
Length: 4883
Transcript: KLK8.gAug05
Reaction
Expected peak: 100
Associated peak: 110,111,112,113,114,115,116
Repeated 51 time
Molarity: 7.23M
Forward: KLK8.F1
Reverse: KLK8.R7
Reaction
Expected peak: 152
Associated peak: 168,169,170,171
Repeated 51 time
Molarity: 8.32M
Forward: KLK8.F1
Reverse: KLK8.R3
Exon
Length: 622
Transcript: KLK8.shed1.aAug05
Exon
Length: 407
Transcript: KLK8.shed2.aAug05
KLK8.R7
CCAGGATGTGATGCCCTG
KLK8.F1
AAGACGTGGATGTTCCTGCT
KLK8.R1
GCATCAGATCATGGTTGTGG
KLK8.R3
AGATGTTGGTATAGACGCCAGG
KLK8.R13
TTGCCACCTACAAGGACACC
Gene primer pair list : Primer pairs
Forward Reverse Occurences View
KLK8.F1 KLK8.R1 51 View
KLK8.F1 KLK8.R13 51 View
KLK8.F1 KLK8.R3 51 View
KLK8.F1 KLK8.R7 51 View

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