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2. Breast Validation Screen 
Transcript map : ECGF1
Exon
Length: 1226
Transcript: ECGF1.aAug05
Exon
Length: 122
Transcript: ECGF1.aAug05
Exon
Length: 117
Transcript: ECGF1.aAug05
Exon
Length: 1065
Transcript: ECGF1.aAug05
Exon
Length: 203
Transcript: ECGF1.aAug05
Exon
Length: 99
Transcript: ECGF1.aAug05
Exon
Length: 130
Transcript: ECGF1.aAug05
Exon
Length: 119
Transcript: ECGF1.aAug05
Exon
Length: 163
Transcript: ECGF1.aAug05
Exon
Length: 231
Transcript: ECGF1.aAug05
Exon
Length: 141
Transcript: ECGF1.aAug05
Exon
Length: 2351
Transcript: ECGF1.aAug05
Intron
Length: 132
Transcript: ECGF1.aAug05
Intron
Length: 108
Transcript: ECGF1.aAug05
Intron
Length: 151
Transcript: ECGF1.aAug05
Intron
Length: 157
Transcript: ECGF1.aAug05
Intron
Length: 525
Transcript: ECGF1.aAug05
Intron
Length: 794
Transcript: ECGF1.aAug05
Intron
Length: 304
Transcript: ECGF1.aAug05
Intron
Length: 426
Transcript: ECGF1.aAug05
Intron
Length: 99
Transcript: ECGF1.aAug05
Intron
Length: 104
Transcript: ECGF1.aAug05
Intron
Length: 82
Transcript: ECGF1.aAug05
Reaction
Expected peak: 551
Associated peak: 536,548,552,553,555,556,558,559,561,562,563,565,567,569,571,573,575,576,577,580,584
Repeated 51 time
Molarity: 1.53M
Forward: ECGF1.F3
Reverse: ECGF1.R30
Reaction
Expected peak: 557
Associated peak: 578,579,580,582,585,586,588,590,591,593,594,597,600,603
Repeated 51 time
Molarity: 1.72M
Forward: ECGF1.F22
Reverse: ECGF1.R27
Exon
Length: 96
Transcript: ECGF1.bAug05
Exon
Length: 259
Transcript: ECGF1.bAug05
Exon
Length: 203
Transcript: ECGF1.bAug05
Exon
Length: 99
Transcript: ECGF1.bAug05
Exon
Length: 130
Transcript: ECGF1.bAug05
Exon
Length: 119
Transcript: ECGF1.bAug05
Exon
Length: 163
Transcript: ECGF1.bAug05
Exon
Length: 231
Transcript: ECGF1.bAug05
Exon
Length: 141
Transcript: ECGF1.bAug05
Exon
Length: 164
Transcript: ECGF1.bAug05
Intron
Length: 149
Transcript: ECGF1.bAug05
Intron
Length: 157
Transcript: ECGF1.bAug05
Intron
Length: 525
Transcript: ECGF1.bAug05
Intron
Length: 794
Transcript: ECGF1.bAug05
Intron
Length: 304
Transcript: ECGF1.bAug05
Intron
Length: 426
Transcript: ECGF1.bAug05
Intron
Length: 99
Transcript: ECGF1.bAug05
Intron
Length: 104
Transcript: ECGF1.bAug05
Intron
Length: 82
Transcript: ECGF1.bAug05
Reaction
Expected peak: 402
Associated peak: 383,384,385,386,387,388,389,390,405,406
Repeated 51 time
Molarity: 13.73M
Forward: ECGF1.F3
Reverse: ECGF1.R30
Exon
Length: 129
Transcript: ECGF1.cAug05
Exon
Length: 236
Transcript: ECGF1.cAug05
Exon
Length: 203
Transcript: ECGF1.cAug05
Exon
Length: 99
Transcript: ECGF1.cAug05
Exon
Length: 130
Transcript: ECGF1.cAug05
Exon
Length: 119
Transcript: ECGF1.cAug05
Exon
Length: 163
Transcript: ECGF1.cAug05
Exon
Length: 231
Transcript: ECGF1.cAug05
Exon
Length: 141
Transcript: ECGF1.cAug05
Exon
Length: 164
Transcript: ECGF1.cAug05
Intron
Length: 172
Transcript: ECGF1.cAug05
Intron
Length: 157
Transcript: ECGF1.cAug05
Intron
Length: 525
Transcript: ECGF1.cAug05
Intron
Length: 794
Transcript: ECGF1.cAug05
Intron
Length: 304
Transcript: ECGF1.cAug05
Intron
Length: 426
Transcript: ECGF1.cAug05
Intron
Length: 99
Transcript: ECGF1.cAug05
Intron
Length: 104
Transcript: ECGF1.cAug05
Intron
Length: 82
Transcript: ECGF1.cAug05
Reaction
Expected peak: 379
Associated peak: 383,384,385,386,387,388,389,390,406
Repeated 51 time
Molarity: 13.74M
Forward: ECGF1.F3
Reverse: ECGF1.R30
Exon
Length: 516
Transcript: ECGF1.dAug05
Exon
Length: 143
Transcript: ECGF1.dAug05
Exon
Length: 204
Transcript: ECGF1.dAug05
Exon
Length: 105
Transcript: ECGF1.dAug05
Exon
Length: 122
Transcript: ECGF1.dAug05
Exon
Length: 141
Transcript: ECGF1.dAug05
Exon
Length: 657
Transcript: ECGF1.dAug05
Exon
Length: 224
Transcript: ECGF1.dAug05
Exon
Length: 203
Transcript: ECGF1.dAug05
Exon
Length: 99
Transcript: ECGF1.dAug05
Exon
Length: 130
Transcript: ECGF1.dAug05
Exon
Length: 119
Transcript: ECGF1.dAug05
Exon
Length: 163
Transcript: ECGF1.dAug05
Exon
Length: 231
Transcript: ECGF1.dAug05
Exon
Length: 141
Transcript: ECGF1.dAug05
Exon
Length: 167
Transcript: ECGF1.dAug05
Intron
Length: 365
Transcript: ECGF1.dAug05
Intron
Length: 195
Transcript: ECGF1.dAug05
Intron
Length: 274
Transcript: ECGF1.dAug05
Intron
Length: 132
Transcript: ECGF1.dAug05
Intron
Length: 84
Transcript: ECGF1.dAug05
Intron
Length: 151
Transcript: ECGF1.dAug05
Intron
Length: 184
Transcript: ECGF1.dAug05
Intron
Length: 157
Transcript: ECGF1.dAug05
Intron
Length: 525
Transcript: ECGF1.dAug05
Intron
Length: 794
Transcript: ECGF1.dAug05
Intron
Length: 304
Transcript: ECGF1.dAug05
Intron
Length: 426
Transcript: ECGF1.dAug05
Intron
Length: 99
Transcript: ECGF1.dAug05
Intron
Length: 104
Transcript: ECGF1.dAug05
Intron
Length: 82
Transcript: ECGF1.dAug05
Reaction
Expected peak: 283
Associated peak: 289,290,291,292,293
Repeated 51 time
Molarity: 24.08M
Forward: ECGF1.F22
Reverse: ECGF1.R27
Reaction
Expected peak: 367
Associated peak: 362,383,384,385,386,387,388,389,390,406
Repeated 51 time
Molarity: 13.81M
Forward: ECGF1.F3
Reverse: ECGF1.R30
Exon
Length: 87
Transcript: ECGF1.eAug05
Exon
Length: 224
Transcript: ECGF1.eAug05
Exon
Length: 203
Transcript: ECGF1.eAug05
Exon
Length: 130
Transcript: ECGF1.eAug05
Exon
Length: 119
Transcript: ECGF1.eAug05
Exon
Length: 163
Transcript: ECGF1.eAug05
Exon
Length: 231
Transcript: ECGF1.eAug05
Exon
Length: 141
Transcript: ECGF1.eAug05
Exon
Length: 134
Transcript: ECGF1.eAug05
Intron
Length: 184
Transcript: ECGF1.eAug05
Intron
Length: 157
Transcript: ECGF1.eAug05
Intron
Length: 1418
Transcript: ECGF1.eAug05
Intron
Length: 304
Transcript: ECGF1.eAug05
Intron
Length: 426
Transcript: ECGF1.eAug05
Intron
Length: 99
Transcript: ECGF1.eAug05
Intron
Length: 104
Transcript: ECGF1.eAug05
Intron
Length: 82
Transcript: ECGF1.eAug05
Reaction
Expected peak: 367
Associated peak: 362,383,384,385,386,387,388,389,390,406
Repeated 51 time
Molarity: 13.81M
Forward: ECGF1.F3
Reverse: ECGF1.R30
Exon
Length: 108
Transcript: ECGF1.fAug05
Exon
Length: 120
Transcript: ECGF1.fAug05
Exon
Length: 203
Transcript: ECGF1.fAug05
Exon
Length: 99
Transcript: ECGF1.fAug05
Exon
Length: 130
Transcript: ECGF1.fAug05
Exon
Length: 164
Transcript: ECGF1.fAug05
Exon
Length: 163
Transcript: ECGF1.fAug05
Exon
Length: 231
Transcript: ECGF1.fAug05
Exon
Length: 141
Transcript: ECGF1.fAug05
Exon
Length: 65
Transcript: ECGF1.fAug05
Intron
Length: 288
Transcript: ECGF1.fAug05
Intron
Length: 157
Transcript: ECGF1.fAug05
Intron
Length: 525
Transcript: ECGF1.fAug05
Intron
Length: 794
Transcript: ECGF1.fAug05
Intron
Length: 259
Transcript: ECGF1.fAug05
Intron
Length: 426
Transcript: ECGF1.fAug05
Intron
Length: 99
Transcript: ECGF1.fAug05
Intron
Length: 104
Transcript: ECGF1.fAug05
Intron
Length: 82
Transcript: ECGF1.fAug05
Reaction
Expected peak: 263
Associated peak: None
Repeated 51 time
Molarity: 0.00M
Forward: ECGF1.F3
Reverse: ECGF1.R30
Exon
Length: 149
Transcript: ECGF1.gAug05
Exon
Length: 224
Transcript: ECGF1.gAug05
Exon
Length: 203
Transcript: ECGF1.gAug05
Exon
Length: 99
Transcript: ECGF1.gAug05
Exon
Length: 130
Transcript: ECGF1.gAug05
Exon
Length: 163
Transcript: ECGF1.gAug05
Exon
Length: 476
Transcript: ECGF1.gAug05
Exon
Length: 167
Transcript: ECGF1.gAug05
Intron
Length: 184
Transcript: ECGF1.gAug05
Intron
Length: 157
Transcript: ECGF1.gAug05
Intron
Length: 525
Transcript: ECGF1.gAug05
Intron
Length: 794
Transcript: ECGF1.gAug05
Intron
Length: 849
Transcript: ECGF1.gAug05
Intron
Length: 99
Transcript: ECGF1.gAug05
Intron
Length: 82
Transcript: ECGF1.gAug05
Reaction
Expected peak: 367
Associated peak: 362,383,384,385,386,387,388,389,390,406
Repeated 51 time
Molarity: 13.81M
Forward: ECGF1.F3
Reverse: ECGF1.R30
Exon
Length: 108
Transcript: ECGF1.hAug05
Exon
Length: 130
Transcript: ECGF1.hAug05
Exon
Length: 119
Transcript: ECGF1.hAug05
Exon
Length: 163
Transcript: ECGF1.hAug05
Exon
Length: 231
Transcript: ECGF1.hAug05
Exon
Length: 156
Transcript: ECGF1.hAug05
Exon
Length: 164
Transcript: ECGF1.hAug05
Intron
Length: 2186
Transcript: ECGF1.hAug05
Intron
Length: 304
Transcript: ECGF1.hAug05
Intron
Length: 426
Transcript: ECGF1.hAug05
Intron
Length: 99
Transcript: ECGF1.hAug05
Intron
Length: 89
Transcript: ECGF1.hAug05
Intron
Length: 82
Transcript: ECGF1.hAug05
Exon
Length: 163
Transcript: ECGF1.iAug05
Exon
Length: 829
Transcript: ECGF1.iAug05
Intron
Length: 1017
Transcript: ECGF1.iAug05
Exon
Length: 135
Transcript: ECGF1.jAug05
Exon
Length: 857
Transcript: ECGF1.jAug05
Intron
Length: 1047
Transcript: ECGF1.jAug05
Exon
Length: 109
Transcript: ECGF1.kAug05
Exon
Length: 2567
Transcript: ECGF1.kAug05
Exon
Length: 56
Transcript: ECGF1.kAug05
Intron
Length: 172
Transcript: ECGF1.kAug05
Intron
Length: 426
Transcript: ECGF1.kAug05
Reaction
Expected peak: 536
Associated peak: 483,484,486,488,536,548,552,553,555,556,559,561,562,563,565,567,569,571,573,575,576,577,580
Repeated 51 time
Molarity: 1.90M
Forward: ECGF1.F3
Reverse: ECGF1.R30
Exon
Length: 216
Transcript: ECGF1.lAug05
Exon
Length: 133
Transcript: ECGF1.lAug05
Exon
Length: 105
Transcript: ECGF1.lAug05
Exon
Length: 122
Transcript: ECGF1.lAug05
Exon
Length: 426
Transcript: ECGF1.lAug05
Intron
Length: 266
Transcript: ECGF1.lAug05
Intron
Length: 274
Transcript: ECGF1.lAug05
Intron
Length: 132
Transcript: ECGF1.lAug05
Intron
Length: 84
Transcript: ECGF1.lAug05
Reaction
Expected peak: 283
Associated peak: 289,290,291,292,293
Repeated 51 time
Molarity: 24.08M
Forward: ECGF1.F22
Reverse: ECGF1.R27
Exon
Length: 184
Transcript: ECGF1.mAug05
Exon
Length: 119
Transcript: ECGF1.mAug05
Exon
Length: 163
Transcript: ECGF1.mAug05
Exon
Length: 231
Transcript: ECGF1.mAug05
Exon
Length: 693
Transcript: ECGF1.mAug05
Intron
Length: 304
Transcript: ECGF1.mAug05
Intron
Length: 426
Transcript: ECGF1.mAug05
Intron
Length: 99
Transcript: ECGF1.mAug05
Intron
Length: 1821
Transcript: ECGF1.mAug05
Exon
Length: 141
Transcript: ECGF1.nAug05
Exon
Length: 519
Transcript: ECGF1.nAug05
Intron
Length: 1576
Transcript: ECGF1.nAug05
Exon
Length: 96
Transcript: ECGF1.oAug05
Exon
Length: 224
Transcript: ECGF1.oAug05
Exon
Length: 341
Transcript: ECGF1.oAug05
Intron
Length: 184
Transcript: ECGF1.oAug05
Intron
Length: 157
Transcript: ECGF1.oAug05
Reaction
Expected peak: 367
Associated peak: 362,383,384,385,386,387,388,389,390,406
Repeated 51 time
Molarity: 13.81M
Forward: ECGF1.F3
Reverse: ECGF1.R30
Exon
Length: 233
Transcript: ECGF1.pAug05
Exon
Length: 632
Transcript: ECGF1.pAug05
Intron
Length: 132
Transcript: ECGF1.pAug05
Reaction
Expected peak: 557
Associated peak: 578,579,580,582,585,586,588,590,591,593,594,597,600,603
Repeated 51 time
Molarity: 1.72M
Forward: ECGF1.F22
Reverse: ECGF1.R27
Exon
Length: 230
Transcript: ECGF1.qAug05
Exon
Length: 600
Transcript: ECGF1.qAug05
Intron
Length: 580
Transcript: ECGF1.qAug05
Exon
Length: 140
Transcript: ECGF1.rAug05
Exon
Length: 105
Transcript: ECGF1.rAug05
Exon
Length: 122
Transcript: ECGF1.rAug05
Exon
Length: 141
Transcript: ECGF1.rAug05
Exon
Length: 135
Transcript: ECGF1.rAug05
Intron
Length: 673
Transcript: ECGF1.rAug05
Intron
Length: 132
Transcript: ECGF1.rAug05
Intron
Length: 84
Transcript: ECGF1.rAug05
Intron
Length: 151
Transcript: ECGF1.rAug05
Exon
Length: 109
Transcript: ECGF1.sAug05
Exon
Length: 471
Transcript: ECGF1.sAug05
Intron
Length: 184
Transcript: ECGF1.sAug05
Reaction
Expected peak: 524
Associated peak: 481,483,484,485,486,488,536,548,552,553,555,556,559,561,562,563,565,567,569,571,573
Repeated 51 time
Molarity: 1.95M
Forward: ECGF1.F3
Reverse: ECGF1.R30
Exon
Length: 980
Transcript: ECGF1.shed1.aAug05
Reaction
Expected peak: 708
Associated peak: 694,700,711,722,723,724,733,734,735,744,746
Repeated 51 time
Molarity: 3.88M
Forward: ECGF1.F3
Reverse: ECGF1.R30
Exon
Length: 73
Transcript: ECGF1.tAug05
Exon
Length: 252
Transcript: ECGF1.tAug05
Exon
Length: 130
Transcript: ECGF1.tAug05
Exon
Length: 20
Transcript: ECGF1.tAug05
Intron
Length: 156
Transcript: ECGF1.tAug05
Intron
Length: 1778
Transcript: ECGF1.tAug05
Intron
Length: 849
Transcript: ECGF1.tAug05
Exon
Length: 122
Transcript: ECGF1.uAug05
Exon
Length: 135
Transcript: ECGF1.uAug05
Intron
Length: 376
Transcript: ECGF1.uAug05
ECGF1.R30
GTCTCCTCCAGATCCATGCC
ECGF1.F22
CCGCTCGCATGACCGTGC
ECGF1.F3
GCGAACCCTGAACCCTACG
ECGF1.R27
CCGTAGATGGAGCAAGTTGG
Gene primer pair list : Primer pairs
Forward Reverse Occurences View
ECGF1.F22 ECGF1.R27 51 View
ECGF1.F3 ECGF1.R30 51 View

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