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Identification of alternative splicing markers for breast cancer
2. Breast Validation Screen
Transcript map
: HSC20
Exon
Length: 301
Transcript: HSC20.aAug05
Length: 301
Transcript: HSC20.aAug05
Exon
Length: 97
Transcript: HSC20.aAug05
Length: 97
Transcript: HSC20.aAug05
Exon
Length: 90
Transcript: HSC20.aAug05
Length: 90
Transcript: HSC20.aAug05
Exon
Length: 145
Transcript: HSC20.aAug05
Length: 145
Transcript: HSC20.aAug05
Exon
Length: 48
Transcript: HSC20.aAug05
Length: 48
Transcript: HSC20.aAug05
Exon
Length: 447
Transcript: HSC20.aAug05
Length: 447
Transcript: HSC20.aAug05
Intron
Length: 1550
Transcript: HSC20.aAug05
Length: 1550
Transcript: HSC20.aAug05
Intron
Length: 636
Transcript: HSC20.aAug05
Length: 636
Transcript: HSC20.aAug05
Intron
Length: 1159
Transcript: HSC20.aAug05
Length: 1159
Transcript: HSC20.aAug05
Intron
Length: 5232
Transcript: HSC20.aAug05
Length: 5232
Transcript: HSC20.aAug05
Intron
Length: 5789
Transcript: HSC20.aAug05
Length: 5789
Transcript: HSC20.aAug05
Reaction
Expected peak: 311
Associated peak: 309,310,311,312,313,314,315,316
Repeated 51 time
Molarity: 7.40M
Forward: HSC20.u.f.8
Reverse: HSC20.u.r.10
Expected peak: 311
Associated peak: 309,310,311,312,313,314,315,316
Repeated 51 time
Molarity: 7.40M
Forward: HSC20.u.f.8
Reverse: HSC20.u.r.10
Reaction
Expected peak: 461
Associated peak: 457,458,460,461,462,463,464,465,466,467,469,472,476,478
Repeated 51 time
Molarity: 3.31M
Forward: HSC20.u.f.10
Reverse: HSC20.au.2.r
Expected peak: 461
Associated peak: 457,458,460,461,462,463,464,465,466,467,469,472,476,478
Repeated 51 time
Molarity: 3.31M
Forward: HSC20.u.f.10
Reverse: HSC20.au.2.r
Reaction
Expected peak: 515
Associated peak: 488,490,491,493,495,496,498
Repeated 51 time
Molarity: 2.21M
Forward: HSC20.u.f.8
Reverse: HSC20.au.2.r
Expected peak: 515
Associated peak: 488,490,491,493,495,496,498
Repeated 51 time
Molarity: 2.21M
Forward: HSC20.u.f.8
Reverse: HSC20.au.2.r
Exon
Length: 181
Transcript: HSC20.bAug05
Length: 181
Transcript: HSC20.bAug05
Exon
Length: 97
Transcript: HSC20.bAug05
Length: 97
Transcript: HSC20.bAug05
Exon
Length: 90
Transcript: HSC20.bAug05
Length: 90
Transcript: HSC20.bAug05
Exon
Length: 276
Transcript: HSC20.bAug05
Length: 276
Transcript: HSC20.bAug05
Exon
Length: 93
Transcript: HSC20.bAug05
Length: 93
Transcript: HSC20.bAug05
Intron
Length: 1550
Transcript: HSC20.bAug05
Length: 1550
Transcript: HSC20.bAug05
Intron
Length: 636
Transcript: HSC20.bAug05
Length: 636
Transcript: HSC20.bAug05
Intron
Length: 551
Transcript: HSC20.bAug05
Length: 551
Transcript: HSC20.bAug05
Intron
Length: 332
Transcript: HSC20.bAug05
Length: 332
Transcript: HSC20.bAug05
Exon
Length: 49
Transcript: HSC20.cAug05
Length: 49
Transcript: HSC20.cAug05
Exon
Length: 97
Transcript: HSC20.cAug05
Length: 97
Transcript: HSC20.cAug05
Exon
Length: 90
Transcript: HSC20.cAug05
Length: 90
Transcript: HSC20.cAug05
Exon
Length: 145
Transcript: HSC20.cAug05
Length: 145
Transcript: HSC20.cAug05
Exon
Length: 48
Transcript: HSC20.cAug05
Length: 48
Transcript: HSC20.cAug05
Exon
Length: 138
Transcript: HSC20.cAug05
Length: 138
Transcript: HSC20.cAug05
Intron
Length: 1153
Transcript: HSC20.cAug05
Length: 1153
Transcript: HSC20.cAug05
Intron
Length: 636
Transcript: HSC20.cAug05
Length: 636
Transcript: HSC20.cAug05
Intron
Length: 1159
Transcript: HSC20.cAug05
Length: 1159
Transcript: HSC20.cAug05
Intron
Length: 5232
Transcript: HSC20.cAug05
Length: 5232
Transcript: HSC20.cAug05
Intron
Length: 5789
Transcript: HSC20.cAug05
Length: 5789
Transcript: HSC20.cAug05
Reaction
Expected peak: 311
Associated peak: 309,310,311,312,313,314,315,316
Repeated 51 time
Molarity: 7.40M
Forward: HSC20.u.f.8
Reverse: HSC20.u.r.10
Expected peak: 311
Associated peak: 309,310,311,312,313,314,315,316
Repeated 51 time
Molarity: 7.40M
Forward: HSC20.u.f.8
Reverse: HSC20.u.r.10
Reaction
Expected peak: 461
Associated peak: 457,458,460,461,462,463,464,465,466,467,469,472,476,478
Repeated 51 time
Molarity: 3.31M
Forward: HSC20.u.f.10
Reverse: HSC20.au.2.r
Expected peak: 461
Associated peak: 457,458,460,461,462,463,464,465,466,467,469,472,476,478
Repeated 51 time
Molarity: 3.31M
Forward: HSC20.u.f.10
Reverse: HSC20.au.2.r
Reaction
Expected peak: 515
Associated peak: 488,490,491,493,495,496,498
Repeated 51 time
Molarity: 2.21M
Forward: HSC20.u.f.8
Reverse: HSC20.au.2.r
Expected peak: 515
Associated peak: 488,490,491,493,495,496,498
Repeated 51 time
Molarity: 2.21M
Forward: HSC20.u.f.8
Reverse: HSC20.au.2.r
Exon
Length: 277
Transcript: HSC20.dAug05
Length: 277
Transcript: HSC20.dAug05
Exon
Length: 97
Transcript: HSC20.dAug05
Length: 97
Transcript: HSC20.dAug05
Exon
Length: 95
Transcript: HSC20.dAug05
Length: 95
Transcript: HSC20.dAug05
Exon
Length: 48
Transcript: HSC20.dAug05
Length: 48
Transcript: HSC20.dAug05
Exon
Length: 438
Transcript: HSC20.dAug05
Length: 438
Transcript: HSC20.dAug05
Intron
Length: 1550
Transcript: HSC20.dAug05
Length: 1550
Transcript: HSC20.dAug05
Intron
Length: 636
Transcript: HSC20.dAug05
Length: 636
Transcript: HSC20.dAug05
Intron
Length: 6531
Transcript: HSC20.dAug05
Length: 6531
Transcript: HSC20.dAug05
Intron
Length: 5789
Transcript: HSC20.dAug05
Length: 5789
Transcript: HSC20.dAug05
Reaction
Expected peak: 171
Associated peak: 178,179,180,181,198
Repeated 51 time
Molarity: 1.20M
Forward: HSC20.u.f.8
Reverse: HSC20.u.r.10
Expected peak: 171
Associated peak: 178,179,180,181,198
Repeated 51 time
Molarity: 1.20M
Forward: HSC20.u.f.8
Reverse: HSC20.u.r.10
Reaction
Expected peak: 321
Associated peak: 312,316,317,318,319,320,322,330,334,335
Repeated 51 time
Molarity: 1.91M
Forward: HSC20.u.f.10
Reverse: HSC20.au.2.r
Expected peak: 321
Associated peak: 312,316,317,318,319,320,322,330,334,335
Repeated 51 time
Molarity: 1.91M
Forward: HSC20.u.f.10
Reverse: HSC20.au.2.r
Reaction
Expected peak: 375
Associated peak: 382,383,384,385,386,387,388,389
Repeated 51 time
Molarity: 0.58M
Forward: HSC20.u.f.8
Reverse: HSC20.au.2.r
Expected peak: 375
Associated peak: 382,383,384,385,386,387,388,389
Repeated 51 time
Molarity: 0.58M
Forward: HSC20.u.f.8
Reverse: HSC20.au.2.r
Exon
Length: 290
Transcript: HSC20.eAug05
Length: 290
Transcript: HSC20.eAug05
Exon
Length: 42
Transcript: HSC20.eAug05
Length: 42
Transcript: HSC20.eAug05
Exon
Length: 90
Transcript: HSC20.eAug05
Length: 90
Transcript: HSC20.eAug05
Exon
Length: 145
Transcript: HSC20.eAug05
Length: 145
Transcript: HSC20.eAug05
Exon
Length: 48
Transcript: HSC20.eAug05
Length: 48
Transcript: HSC20.eAug05
Exon
Length: 425
Transcript: HSC20.eAug05
Length: 425
Transcript: HSC20.eAug05
Intron
Length: 1550
Transcript: HSC20.eAug05
Length: 1550
Transcript: HSC20.eAug05
Intron
Length: 691
Transcript: HSC20.eAug05
Length: 691
Transcript: HSC20.eAug05
Intron
Length: 1159
Transcript: HSC20.eAug05
Length: 1159
Transcript: HSC20.eAug05
Intron
Length: 5232
Transcript: HSC20.eAug05
Length: 5232
Transcript: HSC20.eAug05
Intron
Length: 5789
Transcript: HSC20.eAug05
Length: 5789
Transcript: HSC20.eAug05
Reaction
Expected peak: 461
Associated peak: 457,458,460,461,462,463,464,465,466,467,469,472,476,478
Repeated 51 time
Molarity: 3.31M
Forward: HSC20.u.f.10
Reverse: HSC20.au.2.r
Expected peak: 461
Associated peak: 457,458,460,461,462,463,464,465,466,467,469,472,476,478
Repeated 51 time
Molarity: 3.31M
Forward: HSC20.u.f.10
Reverse: HSC20.au.2.r
Exon
Length: 131
Transcript: HSC20.fAug05
Length: 131
Transcript: HSC20.fAug05
Exon
Length: 97
Transcript: HSC20.fAug05
Length: 97
Transcript: HSC20.fAug05
Exon
Length: 90
Transcript: HSC20.fAug05
Length: 90
Transcript: HSC20.fAug05
Exon
Length: 48
Transcript: HSC20.fAug05
Length: 48
Transcript: HSC20.fAug05
Exon
Length: 136
Transcript: HSC20.fAug05
Length: 136
Transcript: HSC20.fAug05
Intron
Length: 1550
Transcript: HSC20.fAug05
Length: 1550
Transcript: HSC20.fAug05
Intron
Length: 636
Transcript: HSC20.fAug05
Length: 636
Transcript: HSC20.fAug05
Intron
Length: 6536
Transcript: HSC20.fAug05
Length: 6536
Transcript: HSC20.fAug05
Intron
Length: 5789
Transcript: HSC20.fAug05
Length: 5789
Transcript: HSC20.fAug05
Reaction
Expected peak: 166
Associated peak: 178,179,180,181
Repeated 51 time
Molarity: 1.26M
Forward: HSC20.u.f.8
Reverse: HSC20.u.r.10
Expected peak: 166
Associated peak: 178,179,180,181
Repeated 51 time
Molarity: 1.26M
Forward: HSC20.u.f.8
Reverse: HSC20.u.r.10
Reaction
Expected peak: 316
Associated peak: 312,316,317,318,319,320,322,330,334,335
Repeated 51 time
Molarity: 1.91M
Forward: HSC20.u.f.10
Reverse: HSC20.au.2.r
Expected peak: 316
Associated peak: 312,316,317,318,319,320,322,330,334,335
Repeated 51 time
Molarity: 1.91M
Forward: HSC20.u.f.10
Reverse: HSC20.au.2.r
Reaction
Expected peak: 370
Associated peak: 382,383,384,385,386,387,388,389
Repeated 51 time
Molarity: 0.58M
Forward: HSC20.u.f.8
Reverse: HSC20.au.2.r
Expected peak: 370
Associated peak: 382,383,384,385,386,387,388,389
Repeated 51 time
Molarity: 0.58M
Forward: HSC20.u.f.8
Reverse: HSC20.au.2.r
Exon
Length: 28
Transcript: HSC20.gAug05
Length: 28
Transcript: HSC20.gAug05
Exon
Length: 97
Transcript: HSC20.gAug05
Length: 97
Transcript: HSC20.gAug05
Exon
Length: 95
Transcript: HSC20.gAug05
Length: 95
Transcript: HSC20.gAug05
Exon
Length: 145
Transcript: HSC20.gAug05
Length: 145
Transcript: HSC20.gAug05
Exon
Length: 48
Transcript: HSC20.gAug05
Length: 48
Transcript: HSC20.gAug05
Exon
Length: 416
Transcript: HSC20.gAug05
Length: 416
Transcript: HSC20.gAug05
Intron
Length: 1153
Transcript: HSC20.gAug05
Length: 1153
Transcript: HSC20.gAug05
Intron
Length: 636
Transcript: HSC20.gAug05
Length: 636
Transcript: HSC20.gAug05
Intron
Length: 1154
Transcript: HSC20.gAug05
Length: 1154
Transcript: HSC20.gAug05
Intron
Length: 5232
Transcript: HSC20.gAug05
Length: 5232
Transcript: HSC20.gAug05
Intron
Length: 5789
Transcript: HSC20.gAug05
Length: 5789
Transcript: HSC20.gAug05
Reaction
Expected peak: 316
Associated peak: 309,310,311,312,313,314,315,316
Repeated 51 time
Molarity: 7.40M
Forward: HSC20.u.f.8
Reverse: HSC20.u.r.10
Expected peak: 316
Associated peak: 309,310,311,312,313,314,315,316
Repeated 51 time
Molarity: 7.40M
Forward: HSC20.u.f.8
Reverse: HSC20.u.r.10
Reaction
Expected peak: 466
Associated peak: 457,458,460,461,462,463,464,465,466,467,469,472,476,478
Repeated 51 time
Molarity: 3.31M
Forward: HSC20.u.f.10
Reverse: HSC20.au.2.r
Expected peak: 466
Associated peak: 457,458,460,461,462,463,464,465,466,467,469,472,476,478
Repeated 51 time
Molarity: 3.31M
Forward: HSC20.u.f.10
Reverse: HSC20.au.2.r
Reaction
Expected peak: 520
Associated peak: 488,490,491,493,495,496,498
Repeated 51 time
Molarity: 2.21M
Forward: HSC20.u.f.8
Reverse: HSC20.au.2.r
Expected peak: 520
Associated peak: 488,490,491,493,495,496,498
Repeated 51 time
Molarity: 2.21M
Forward: HSC20.u.f.8
Reverse: HSC20.au.2.r
Exon
Length: 431
Transcript: HSC20.shed1.aAug05
Length: 431
Transcript: HSC20.shed1.aAug05
Exon
Length: 341
Transcript: HSC20.shed2.aAug05
Length: 341
Transcript: HSC20.shed2.aAug05
Exon
Length: 541
Transcript: HSC20.shed3.aAug05
Length: 541
Transcript: HSC20.shed3.aAug05
Exon
Length: 236
Transcript: HSC20.shed4.aAug05
Length: 236
Transcript: HSC20.shed4.aAug05
HSC20.au.2.r
TTGTCATCCACCCACCTCA
TTGTCATCCACCCACCTCA
HSC20.u.f.8
CGTCTTGTCCACCCAGATTT
CGTCTTGTCCACCCAGATTT
HSC20.u.f.10
TCAGAGAAGCATTCGACCCT
TCAGAGAAGCATTCGACCCT
HSC20.u.r.10
GCACTGCTCACATTGTCAGTAAA
GCACTGCTCACATTGTCAGTAAA