Supplementary table 1: Training set primer sequences

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Supplementary table 1: Training set primer sequences ⚙

Information : Data view

Gene name	BSP 5' end sequence	BSP 3' end sequence	ΔCt	3’ end mispriming	Specificity for typical qRT-PCR
AXIN1	GCACCCTCCAAGCAGAG	GAC	17.6	yes	yes
	ACCCTCCAAGCAGAG	GACAA	20,8*	no	yes
	CCCTCCAAGCAGAG	GACAAGA	>17,7**	no	yes
	CCCTCCAAGCAGAG	GACAAGAT	17.6	yes	yes
	CCCTCCAAGCAGAG	GACAAGATC	11.8	yes	no
	CCAAGCAGAG	GACAAGATCGC	5.5	yes	no
	CAAGCAGAG	GACAAGATCGCAG	3.1	yes	no
BCAS1	GTCTTTTCTGTGGAGTCTGATGT	CAT	>26,0	no	yes
	TCTTTTCTGTGGAGTCTGATGT	CATTT	>27,5	no	yes
	TTTCTGTGGAGTCTGATGT	CATTTGT	>30,0	no	yes
	CTTTTCTGTGGAGTCTGATGT	CATTTGTC	22.4	yes	yes
	CTGTGGAGTCTGATGT	CATTTGTCT	18	yes	yes
	GTGGAGTCTGATGT	CATTTGTCTGA	13.9	yes	no
	TGGAGTCTGATGT	CATTTGTCTGAGA	11.1	yes	no
	GGAGTCTGATGT	CATTTGTCTGAGAAA	4.8	yes	no
BTC	GACGCCCTCCTGTGT	CCC	>16,4	no	yes
	CGCCCTCCTGTGT	CCCTC	>21,1	no	yes
	GCCCTCCTGTGT	CCCTCTT	>29,2	no	yes
	GCCCTCCTGTGT	CCCTCTTC	21.1	yes	yes
	CCCTCCTGTGT	CCCTCTTCG	12.8	yes	no
	CTCCTGTGT	CCCTCTTCGGA	8.4	yes	no
	TCCTGTGT	CCCTCTTCGGAAA	6.9	yes	no
PCSK6	CGTAGCTGGCGTAGGAATC	CAG	>32,1	no	yes
	GCTGGCGTAGGAATC	CAGGT	>32,1	no	yes
	GCTGGCGTAGGAATC	CAGGTAC	23.5	yes	yes
	CTGGCGTAGGAATC	CAGGTACC	18.2	yes	yes
	GGCGTAGGAATC	CAGGTACCA	13.4	yes	no
	GCGTAGGAATC	CAGGTACCACA	9.5	yes	no
	CGTAGGAATC	CAGGTACCACATG	6.0	yes	no
	CGTAGGAATC	CAGGTACCACATGTT	3.3	yes	no

* 5' end mispriming observed
** primer-dimers observed

Primers to generate the templates (common fixed opposing primer in bold)

Gene name	Forward	Reverse
AXIN1	ACCCTCCAAGCAGAGGTATGT	TAGCTGCCCTTTTTGGTCAGCA
BCAS1	AACAGTGGACCTCAACGAAGGA	AGTCTGATGTTTGGCAGCTGGA
BTC	TTGGTCATCGGTGTCTGCACAT	ACTTTGCAGCTTGCCACCAA
PCSK6	TGAAATGGCTCCAGCAACAGGA	CACGCTGCCTGGACATTCATT