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LGFUS Public Data
High-Throughput Quantification of Splicing Isoforms
Supplementary table 3: Mismatch combination set primer sequences
Information
: Data view
| Gene name | Forward primer sequence | Reverse primer sequence | BSP mismatch rule | Mismatch type | Specificity for typical qRT-PCR |
| HSC20 | GAGCAGAGGACTGTACCTTCTAAAC | CATCTTGTTCAAAAGCACTGCTCAC | /MMMMMX | C/C | yes |
| LIG3 | GCGGCCTGTAATGAGCAAGTT | GTCCCACATGCAACCCTGC | /MMMX | C/C | yes |
| RUNX2 | ACTCTACCACCCCGCTGTCTT | GCCCAGTTCTGAAGCACCTGA | /MMMX | A/G | yes |
| TLK1 | CGAAAGCTCTAATCACAGTTTTGGAAGC | GATTGTTTCTTCTCCGGTGTCTCT | /MMMX | T/T | yes |
| TSSC4 | TCCAGTGTCAACCACTGGCAC | GGCTGGGAGGAGGCCTGT | /MMMX | T/T | yes |
| PAXIP1 | GCTTGGAAGAATCCTTAAAACGGGC | CCAGGTGTGATGTAAAAATATTTTGCCTT | /MMX | T/C | yes |
| POLB | AATCACCGACATGCTCACAGAAA | CAGGCAATTTCTTAGCTTCAGCTCC | /MMX | A/G | yes |
| FGFR2 | AAGGCATTGCGCGTAGTCCA | CTCCGGATGAGATGGCATCTTC | /MMXX | T/C C/A | yes |
| FN1-intron | ACTATTGAAGGCTTGCAGCCCA | CAGTTGGTGCAGGAATAGTGGTTA | /MMXX | A/C C/A | yes |
| NRG1 | AATGGCCAGCTTCTACAAGGC | GTCATGCAGCTTTTTCCGCTGT | /MMXX | G/G C/T | yes |
| POLB | TCACCGACATGCTCACAGAAAA | GCAATTTTTGTTCCTACTCCAGGC | /MMXX | G/A A/A | yes |
| AXIN1 | GCACCCTCCAAGCAGAGGA | TACGCCACAACGATGCTGTCA | /MX | A/A | yes |
| BMP4 | GCAGAGGAGGAGGGAGGGA | GGCACTACGGAATGGCTCCT | /MX | T/T | yes |
| DDR1 | AGAGCCACCCCCGTACCA | CCATATAGTCCCCACTGTAGGCA | /MX | A/G | yes |
| ECGF1 | CCGCCTACTCCATCTACGGC | CTCCGGGAAGTACCTGCCA | /MX | A/A | yes |
| IGSF4 | TATCCCTCCTCCCACAACAACCA | TCTTCACCTGCTCGGGAATCT | /MX | T/C | yes |
| MCL1 | CGAGACGGCCTTCCAAGGA | AACACCTGCAAAAGCCAGCA | /MX | A/G | yes |
| PTPN13 | ATCAGACTCCTCATCCATTGAAGACC | GCTCCATCTTTTGTGTAGCACATCT | /MX | T/T | yes |
| SHMT1 | TTGAACACTGCCATGTGGTGAC | AGTGCCACAGCAACCCCT | /MX | T/T | yes |
| SYNE2 | TCCTCTCACGAAGAGGACGAG | GGCCATCCGAAATGGATTTACCT | /MX | T/T | yes |
| APP | TGTGTGGCAGCGCCATTC | TGACGTTCTGCCTCTTCCCATT | /MXX | T/C C/A | yes |
| AXIN1 | GCACCCTCCAAGCAGAGGAC | TAGCTGCCCTTTTTGGTCAGCA | /MXX | A/A C/T | yes |
| BTC | GACGCCCTCCTGTGTCCC | ACTTTGCAGCTTGCCACCAA | /MXX | C/A C/C | yes |
| CHEK2 | GCTGGCTTTCGAGAGGAAAACA | AGCTCTCCCCCTTCCATCATG | /MXX | T/T A/G | yes |
| MCL1 | CGAGACGGCCTTCCAAGGAT | TGCCACTTGCTTTTCTGGCT | /MXX | A/G T/T | yes |
| PCSK6 | CCAGCAACAGGAAGTGAAACGA | CGTAGCTGGCGTAGGAATCCAG | /MXX | A/G G/G | yes |
| SHMT1 | AAGCTGATCATCGCAGGAACCA | GTGCCACAGCAACCCCTT | /MXX | T/T T/C | yes |
| SYK | AGGGAAAGAAGTTCGACACGCT | TTGGGCAGGGGAGGACGC | /MXX | G/A C/A | yes |
| AFF3 | GGGACCTCGAGTCACTGTGT | GTGCCATCATCCTGTTGAGTTTCT | /X | T/C | yes |
| APP | TCTTTTACGGCGGATGTGGC | GTACTGGCTGCTGTTGTAGGAAT | /X | T/C | yes |
| BCAS1 | TGATGGCGTTTCTCAGACAAATGA | TTCTGTGGTGCTCCTGTTGGTT | /X | A/A | yes |
| BCMP11 | GAGCACTGGCCAAGTCAGG | TGAGCATAAAAGAGACCTTCTTCATAAGT | /X | G/G | yes |
| BTC | TGCCCCAAGCAATACAAGCA | ACGACGTTTCCGAAGAGGGA | /X | A/A | yes |
| CD40 | TCGCTATGGTTCGTCTGCCT | TGGGTGGTTCTGGATGGACA | /X | A/G | yes |
| DRF1 | TCCTCAAAATCGAAGATGAAAGCAGA | TTCTCTGGTATGGTGCATGCTG | /X | A/C | yes |
| DSC3 | TCAACCCCGTCTCGGTGAAA | ATAATCTTGGGATGGCATGCGG | /X | A/C | yes |
| FANCA | TGATGCTCTTTCAGATACTGAACGC | ATTTCCTGTGCGGCCACCAA | /X | C/C | yes |
| GNB3 | AGATGGAGCAACTGCGTCAGG | GCACAGGCTTTCCTGGCATCT | /X | T/G | yes |
| INSR | CGTGGTTTTCGTCCCCAGG | GTGTTGGGGAAAGCTGCCAC | /X | G/T | yes |
| NOTCH3 | CCCCCAGGGCTGGCAAGA | AGCAGGAAAAGGAGCCCACG | /X | A/C | yes |
| PCSK6 | GGTCCAACATGTGGTACCTGG | TGCTGGCATCATATCGTGGAGA | /X | G/G | yes |
| PLD1 | CAGCATTGACAGCACCTCCAA | TGGTTTCCCCATGCAGCTCT | /X | A/C | yes |
| RSN | AGAATCAATTACCAAAGGTGATCTTGAGA | TGTTCAAGCTCCTTAATGCGGG | /X | A/G | yes |
| STIM1 | ATGACTGACGACGTGGATGACA | GCTCTGCAGGCTAGGGGA | /X | A/G | yes |
| SYK | CCAGGTTCCCATCCTGCGT | TGCAAGTTCTGGCTCATACGGA | /X | T/T | yes |
| TOPBP1 | ACCAGCCAGATCAACACAATTGATAA | TGATTCACTTACGCAACTTGCATTT | /X | A/C | yes |
| PTPRB | CCTGTACTCCGTGGTGGTAACAA | CTGGACTTTGTCAGGCACTGTT | /MMMMX | T/G | no |
| LIG4 | TGGCGGAACCGGCATCTTCT | CGGTGATGAATCTTCTCGTTTAACTGA | /MMMX | A/C | no |
| C11orf17 | CCCCACCTAGAGAAACAGCCG | TTCCTCTGGCACAGATGAATAATATTTCT | /MX | T/G | no |
| NUP98 | AGCGGAGGAGCAAAACTCACA | GAACAGCCTTCTCACGTATGCT | /MX | T/G | no |
| SHC1 | ATGCCAATCACTCTCACCGTCT | CGACATACTCGGCTGTGTCCT | /MX | T/C | no |
| APC | GCAGTTGGACTTAACGTATTTCTTGAGG | TCAAGTGCAGCCATCTTGCCA | /X | G/T | no |
| CASC4 | AAAGCAGGCTACCAAGGACAGA | CATTTGAAGCTCTCGTTCTTCATCATT | /X | T/G | no |
| CCL4 | TGCGTGACTGTCCTGTCTCT | TGCTTGCTTCTTTTGGTTTGGAATT | /X | T/G | no |
| FGFR2 | AGGATACCACATTAGAGCCAGAAGAT | ACAAAATCTTCCGCACCATCGG | /X | T/C | no |
| KITLG | GTTTCTTCAACATTAAGTCCTGAGAAAGG | TGCCCAGTGTAGGCTGGAGT | /X | G/T | no |
| NRG1 | GCCCAAATGAGTTTACTGGTGATCG | GGTACAGCTCCTCCGCCTT | /X | T/G | no |
| UTRN | TTCTTGAAGGTGACAACTTAGAGACC | GCCAGTCGTGTGGCATATTGTT | /X | C/T | no |
| VLDLR | CTCCAAAATATACCTGTTCCTGTCCC | CTGCTGTGGTCACATTGATCCT | /X | T/G | no |
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