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PNT2 siRNA validations 
Transcript map : APH1B Carstens_siESRP
Exon
Length: 143
Transcript: APH1B.aApr07
Exon
Length: 171
Transcript: APH1B.aApr07
Exon
Length: 71
Transcript: APH1B.aApr07
Exon
Length: 123
Transcript: APH1B.aApr07
Exon
Length: 128
Transcript: APH1B.aApr07
Exon
Length: 163
Transcript: APH1B.aApr07
Intron
Length: 3000
Transcript: APH1B.aApr07
Intron
Length: 7226
Transcript: APH1B.aApr07
Intron
Length: 795
Transcript: APH1B.aApr07
Intron
Length: 14798
Transcript: APH1B.aApr07
Intron
Length: 3141
Transcript: APH1B.aApr07
Reaction
Expected peak: 268
24M
APH1B.E.1.F2
APH1B.E.1.R2
Reaction
Expected peak: 305
25.9M
APH1B.E.1.F1
APH1B.E.1.R1
Exon
Length: 217
Transcript: APH1B.bApr07
Exon
Length: 171
Transcript: APH1B.bApr07
Exon
Length: 71
Transcript: APH1B.bApr07
Exon
Length: 123
Transcript: APH1B.bApr07
Exon
Length: 128
Transcript: APH1B.bApr07
Exon
Length: 3513
Transcript: APH1B.bApr07
Intron
Length: 1424
Transcript: APH1B.bApr07
Intron
Length: 7226
Transcript: APH1B.bApr07
Intron
Length: 795
Transcript: APH1B.bApr07
Intron
Length: 14798
Transcript: APH1B.bApr07
Intron
Length: 3141
Transcript: APH1B.bApr07
Reaction
Expected peak: 268
24M
APH1B.E.1.F2
APH1B.E.1.R2
Reaction
Expected peak: 305
25.9M
APH1B.E.1.F1
APH1B.E.1.R1
Exon
Length: 119
Transcript: APH1B.cApr07
Exon
Length: 171
Transcript: APH1B.cApr07
Exon
Length: 71
Transcript: APH1B.cApr07
Exon
Length: 123
Transcript: APH1B.cApr07
Exon
Length: 203
Transcript: APH1B.cApr07
Intron
Length: 1424
Transcript: APH1B.cApr07
Intron
Length: 7226
Transcript: APH1B.cApr07
Intron
Length: 795
Transcript: APH1B.cApr07
Intron
Length: 18067
Transcript: APH1B.cApr07
Exon
Length: 131
Transcript: APH1B.dApr07
Exon
Length: 171
Transcript: APH1B.dApr07
Exon
Length: 71
Transcript: APH1B.dApr07
Exon
Length: 128
Transcript: APH1B.dApr07
Exon
Length: 380
Transcript: APH1B.dApr07
Intron
Length: 1424
Transcript: APH1B.dApr07
Intron
Length: 7226
Transcript: APH1B.dApr07
Intron
Length: 15716
Transcript: APH1B.dApr07
Intron
Length: 3141
Transcript: APH1B.dApr07
Reaction
Expected peak: 145
12.7M
APH1B.E.1.F2
APH1B.E.1.R2
Reaction
Expected peak: 182
12.4M
APH1B.E.1.F1
APH1B.E.1.R1
Exon
Length: 125
Transcript: APH1B.eApr07
Exon
Length: 64
Transcript: APH1B.eApr07
Exon
Length: 171
Transcript: APH1B.eApr07
Exon
Length: 71
Transcript: APH1B.eApr07
Exon
Length: 123
Transcript: APH1B.eApr07
Exon
Length: 128
Transcript: APH1B.eApr07
Exon
Length: 189
Transcript: APH1B.eApr07
Intron
Length: 554
Transcript: APH1B.eApr07
Intron
Length: 806
Transcript: APH1B.eApr07
Intron
Length: 7226
Transcript: APH1B.eApr07
Intron
Length: 795
Transcript: APH1B.eApr07
Intron
Length: 14798
Transcript: APH1B.eApr07
Intron
Length: 3141
Transcript: APH1B.eApr07
Reaction
Expected peak: 268
24M
APH1B.E.1.F2
APH1B.E.1.R2
Reaction
Expected peak: 305
25.9M
APH1B.E.1.F1
APH1B.E.1.R1
Exon
Length: 312
Transcript: APH1B.fApr07
Exon
Length: 128
Transcript: APH1B.fApr07
Exon
Length: 291
Transcript: APH1B.fApr07
Intron
Length: 10347
Transcript: APH1B.fApr07
Intron
Length: 3141
Transcript: APH1B.fApr07
Exon
Length: 366
Transcript: APH1B.gApr07
Exon
Length: 149
Transcript: APH1B.gApr07
Intron
Length: 14798
Transcript: APH1B.gApr07
Exon
Length: 246
Transcript: APH1B.hApr07
Exon
Length: 171
Transcript: APH1B.hApr07
Exon
Length: 71
Transcript: APH1B.hApr07
Exon
Length: 55
Transcript: APH1B.hApr07
Intron
Length: 1231
Transcript: APH1B.hApr07
Intron
Length: 7226
Transcript: APH1B.hApr07
Intron
Length: 795
Transcript: APH1B.hApr07
Exon
Length: 4799
Transcript: APH1B.iApr07-unspliced
Exon
Length: 245
Transcript: APH1B.jApr07
Exon
Length: 227
Transcript: APH1B.jApr07
Intron
Length: 363
Transcript: APH1B.jApr07
APH1B.E.1.F1
AGCCAGTGAAGGTTTGAAGAGT
APH1B.E.1.F2
GTGAGACAGCACCCTCTATGCG
APH1B.E.1.R2
TGGGTCAGGAGAACGATAAGGA
APH1B.E.1.R1
GCAGGTGGGTCAGGAGAACG

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