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Home Supplementary data Tumor microenvironment associated modifications of alternative splicing Tissu quality control molecular markers (related to Fig. 1B and Table S1) LCM discovery and validation sample set permalink
LCM discovery and validation sample set ⚙
Reference Genes : primer pair list
Gene Forward Reverse
VIEW B2M B2M_G_3_f B2M_G_3_r
VIEW GAPDH GAPDH_G_f GAPDH_G_r
VIEW HMBS HMBS_G_3_f HMBS_G_3_r
VIEW MRPL19 MRPL19_G_3_f MRPL19_G_3_r
VIEW PSMC4 PSMC4_G_1_f PSMC4_G_1_r
VIEW PUM1 PUM1_G_2_f PUM1_G_2_r
VIEW SDHA SDHA_G_f SDHA_G_r
VIEW YWHAZ YWHAZ_G_f YWHAZ_G_r
QPCR Relative Expression Quantification : NF pairwise rate map
QPCR Relative Expression Quantification : NF Histogram
QPCR Relative Expression Quantification : Reference Genes list

Reference Genes For Relative Expression Analysis

  • PUM1_G_2_f PUM1_G_2_r
  • GAPDH_G_f GAPDH_G_r
  • YWHAZ_G_f YWHAZ_G_r
QPCR Relative Expression Quantification : Reports

Comments on the experiments

  • same sample as discovery screen LCM epithelial but bulk
  • same sample as discovery screen LCM epithelial but bulk
  • same sample as discovery screen LCM epithelial
  • same sample as discovery screen LCM epithelial
  • same sample as discovery screen LCM epithelial
  • same sample as discovery screen LCM epithelial
  • same sample as discovery screen LCM epithelial
  • same sample as discovery screen LCM epithelial
  • same sample as discovery screen LCM epithelial
  • same sample as discovery screen LCM epithelial
  • same sample as discovery screen LCM epithelial
  • same sample as discovery screen LCM epithelial
  • same sample as discovery screen LCM epithelial
  • same sample as discovery screen LCM epithelial
QPCR Genorm : M -- average pairwise expression ratio
Primer Pair 8 7 6 5 4 3 2 1
SDHA_G_f SDHA_G_r 2.771
HMBS_G_3_f HMBS_G_3_r 2.377 2.209
MRPL19_G_3_f MRPL19_G_3_r 1.765 1.595 1.479
PSMC4_G_1_f PSMC4_G_1_r 1.584 1.394 1.210 1.108
B2M_G_3_f B2M_G_3_r 1.587 1.385 1.214 1.084 0.986
PUM1_G_2_f PUM1_G_2_r 1.504 1.274 1.053 0.912 0.980 0.980
YWHAZ_G_f YWHAZ_G_r 1.357 1.172 0.983 0.921 0.809 0.809
GAPDH_G_f GAPDH_G_r 1.292 1.114 0.931 0.865 0.790 0.790

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