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Supplementary data
Tumor microenvironment associated modifications of alternative splicing
Quantitative RT-PCR primer validation
ASEs
Test in FFPE pool (additional)
BSP Analysis
: FT OVN ext NA primer validation
FT OVN ext NA primer validation
| Event | PCR Hit | Detection | Primers | Primer test | BSP test | E | |||||||||
| Forward name | Reverse name | Forward seq | Reverse seq | Curve | NTC | CGE | Sequence | Conclusion | CT diff | NTC | Conclusion | ||||
| ABCC11 | short | ABCC11_cass_S_f | ABCC11_cass_S_r | CATTCCTGACCAAGGCCATCAT | TCACGGATTGTGCGCTGGAT | BAD | BAD | BAD | NR | BAD | - | - | - | 1.5 +/- 0 | |
| ABCC11 | short | ABCC11_cass_S_f | ABCC11_cass_S_1_r | CATTCCTGACCAAGGCCATCAT | TTCACGGATTGTGCGCTGGAT | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.51 +/- 0 | |
| ACRV1 | short | ACRV1_cass_S_f | ACRV1_cass_S_1_r | CCAATTTCAAGCACATCTACAGGTG | CGTTCCATGGGAGAAGAGGTTCAT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.07 +/- 0 | |
| ACRV1 | short | ACRV1_cass_S_f | ACRV1_cass_S_r | CCAATTTCAAGCACATCTACAGGTG | TCGTTCCATGGGAGAAGAGGTT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.08 +/- 0 | |
| AGC1 | short | AGC1_cass_S_3_f | AGC1_cass_S_1_r | TGATGATCTGGCACGAGAAGGG | GCGTTTGTAGGTGGTGGCT | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.49 +/- 0 | |
| AGC1 | short | AGC1_cass_S_2_f | AGC1_cass_S_1_r | ATGATCTGGCACGAGAAGGGC | GCGTTTGTAGGTGGTGGCT | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.51 +/- 0 | |
| AKAP14 | long | AKAP14_cass_L_f | AKAP14_cass_L_r | TGAATTCACTGTGGAAAAGGGTCTT | CGCCATGTGCCCAGCATTTT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.53 +/- 0 | |
| AKAP14 | long | AKAP14_cass_L_f | AKAP14_cass_L_1_r | TGAATTCACTGTGGAAAAGGGTCTT | TACGCCATGTGCCCAGCATT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.56 +/- 0 | |
| AKAP14 | short | AKAP14_cass_S_f | AKAP14_cass_S_r | AACATCAAGTGGATGACTCACGG | ACAACAATGGGTGCATCCGAA | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.47 +/- 0 | |
| AKAP14 | short | AKAP14_cass_S_1_f | AKAP14_cass_S_r | AAACATCAAGTGGATGACTCACGG | ACAACAATGGGTGCATCCGAA | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.51 +/- 0 | |
| C18orf34 | long | C18orf34_cass_L_f | C18orf34_cass_L_1_r | AGGGGAAGAAAACCCTTGATGC | TCTAGGTCTTTAAAAATTGCTGAGCGTT | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.29 +/- 0 | |
| C18orf34 | long | C18orf34_cass_L_f | C18orf34_cass_L_r | AGGGGAAGAAAACCCTTGATGC | TCTAGGTCTTTAAAAATTGCTGAGCGT | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.4 +/- 0 | |
| C18orf34 | short | C18orf34_cass_S_f | C18orf34_cass_S_r | AGTCTCGACAAAGAACATTCTGTTAGT | TCATTGTCTTACTTTTAGTTGCTTCTAGGT | BAD | GOOD | GOOD | NR | BAD | - | - | - | 1.05 +/- 0 | |
| C18orf34 | short | C18orf34_cass_S_1_f | C18orf34_cass_S_1_r | CAACTAGAAGATGAAGCTGAAAGAATCAG | AAAAATTGCTGAGCGTTTACTAACAGAA | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.04 +/- 0 | |
| C1orf178 | short | C1orf178_cass_S_1_f | C1orf178_cass_S_r | CAACATTGCAGGTTGCCAGC | CCTGGAGTATAGCTCCTGTCTGAA | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.03 +/- 0 | |
| C1orf178 | short | C1orf178_cass_S_f | C1orf178_cass_S_r | CCCAACATTGCAGGTTGCCA | CCTGGAGTATAGCTCCTGTCTGAA | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.03 +/- 0 | |
| C9orf24 | short | C9orf24_alt5_S_f | C9orf24_alt5_S_r | AGCGCGAAGTGATGGTGAACA | GCTCGTAGGGAGGTACACATCC | GOOD | BAD | GOOD | NR | BAD | - | - | - | 1.45 +/- 0 | |
| C9orf24 | short | C9orf24_alt5_S_1_f | C9orf24_alt5_S_r | AACATGCTGAACTCACTGTCGC | GCTCGTAGGGAGGTACACATCC | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.43 +/- 0 | |
| CABP1 | long | CABP1_cass_L_f | CABP1_cass_L_r | GGGCAACTGTGTCAAGTATCCA | ACCATGTAGCTGGTCTGTTCCT | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.45 +/- 0 | |
| CABP1 | long | CABP1_cass_S_f | CABP1_cass_L_r | AAGAGGGGCTGCCTCAACTTA | ACCATGTAGCTGGTCTGTTCCT | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.47 +/- 0 | |
| CABP1 | short | CABP1_cass_S_1_f | CABP1_cass_S_r | AGAGGGGCTGCCTCAACTTA | CTCTGGTCGCAGTGATCTATCCT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.06 +/- 0 | |
| CABP1 | short | CABP1_cass_S_f | CABP1_cass_S_r | AAGAGGGGCTGCCTCAACTTA | CTCTGGTCGCAGTGATCTATCCT | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.41 +/- 0 | |
| CACNG6 | short | CACNG6_cass_S_1_f | CACNG6_cass_S_r | GCAAACTGCACCTATTTTAAATTCTTCAC | CCAAGAGCAGCAGGCCTTT | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.5 +/- 0 | |
| CACNG6 | short | CACNG6_cass_S_f | CACNG6_cass_S_r | GCAAACTGCACCTATTTTAAATTCTTCACC | CCAAGAGCAGCAGGCCTTT | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.24 +/- 0 | |
| CAMK2A | long | CAMK2A_cass_L_f | CAMK2A_cass_S_r | AGTCCAGTTCCAGCGTTCAGTT | TCCGCACTTTGGTGTCTTCATC | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.52 +/- 0 | |
| CAMK2A | long | CAMK2A_cass_L_1_f | CAMK2A_cass_S_r | AAGTCCAGTTCCAGCGTTCAGT | TCCGCACTTTGGTGTCTTCATC | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.51 +/- 0 | |
| CCNC | short | CCNC_alt5_S_f | CCNC_alt5_S_r | GTCAGCACCTCCCCTCCG | CTTGTTTATCCAAAATCCATTGCAAATGT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.06 +/- 0 | |
| CCNC | short | CCNC_alt5_S_1_f | CCNC_alt5_S_r | GGTCAGCACCTCCCCTCC | CTTGTTTATCCAAAATCCATTGCAAATGT | GOOD | BAD | BAD | NR | BAD | - | - | - | 1.1 +/- 0 | |
| CD79A | short | CD79A_alt5_S_f | CD79A_alt5_S_r | GGGCGAGGACCCCAATGAG | TCGGCTGTGATGATTCGGTTCT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.05 +/- 0 | |
| CD79A | short | CD79A_alt5_S_f | CD79A_alt5_S_1_r | GGGCGAGGACCCCAATGAG | CTCGGCTGTGATGATTCGGTT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.04 +/- 0 | |
| CERKL | long | CERKL_cass_L_f | CERKL_cass_L_r | CTGGCATGCAAACGTTAGAACC | TCAGAAGCAAAGCATGGGCTAC | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.5 +/- 0 | |
| CERKL | long | CERKL_cass_L_f | CERKL_cass_S_r | CTGGCATGCAAACGTTAGAACC | CAAAGCATGGGCTACTTCGCT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.47 +/- 0 | |
| CLEC4M | short | CLEC4M_cass_S_f | CLEC4M_cass_S_r | CAAAACTGCTGAGGAGCAGCT | GTCTTCATTCCCGCTATTGTTGGG | BAD | GOOD | GOOD | NR | BAD | - | - | - | 1.42 +/- 0 | |
| CLEC4M | short | CLEC4M_cass_S_f | CLEC4M_cass_S_1_r | CAAAACTGCTGAGGAGCAGCT | GCACAGTCTTCATTCCCGCTAT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.4 +/- 0 | |
| CMTM5 | short | CMTM5_cass_S_f | CMTM5_cass_S_r | GAATTAACTGGCCCTGTCTGGTT | AGTCCGGTAGATCTTGAAGGCA | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.06 +/- 0 | |
| CMTM5 | short | CMTM5_cass_S_f | CMTM5_cass_S_1_r | GAATTAACTGGCCCTGTCTGGTT | CAGTCCGGTAGATCTTGAAGGCAT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.04 +/- 0 | |
| COLQ | short | COLQ_cass_S_3_f | COLQ_cass_S_4_r | AGGACCAAAGGGAGAAAAGGGTA | AGGGCCTGGCCAACCGAT | BAD | GOOD | GOOD | NR | BAD | - | - | - | 1.22 +/- 0 | |
| COLQ | short | COLQ_cass_S_3_f | COLQ_cass_S_3_r | AGGACCAAAGGGAGAAAAGGGTA | GGGCCTGGCCAACCGATG | BAD | GOOD | GOOD | NR | BAD | - | - | - | 1.16 +/- 0 | |
| DUOX1 | long | DUOX1_cass_L_1_f | DUOX1_cass_L_r | GGAGACAAGTTGCAGTCCCG | TCTCCAATCTGATCCTTGTTTTCCTC | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.04 +/- 0 | |
| DUOX1 | long | DUOX1_cass_L_f | DUOX1_cass_L_r | CGGAGACAAGTTGCAGTCCC | TCTCCAATCTGATCCTTGTTTTCCTC | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.03 +/- 0 | |
| DUOX1 | short | DUOX1_cass_S_f | DUOX1_cass_S_r | GGACGAGAGAGGGCTCCG | CCAAAATGGAGACCCTGCGG | GOOD | BAD | BAD | NR | BAD | - | - | - | 1.44 +/- 0.01 | |
| EBF2 | short | EBF2_cass_S_f | EBF2_cass_S_1_r | GACGCACGAAGTGATGTGTAGAT | CCGTCTCATGTCCCTTGGGTT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.02 +/- 0 | |
| EBF2 | short | EBF2_cass_S_f | EBF2_cass_S_r | GACGCACGAAGTGATGTGTAGAT | CCGTCTCATGTCCCTTGGGTTT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.02 +/- 0 | |
| EMR3 | EMR3_cass_L_f | EMR3_cass_S_r | TGTGTCCCAGGATATAGACTGCAT | TGTCCACAATCTTTTGCAGCCT | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.52 +/- 0 | ||
| EMR3 | long | EMR3_cass_L_f | EMR3_cass_L_1_r | TGTGTCCCAGGATATAGACTGCAT | TCGGTTGTCTTTGAGGAGGTGG | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.52 +/- 0 | |
| EMR3 | short | EMR3_cass_S_f | EMR3_cass_S_r | CTGTCAATGTGTCCCAGGATATAGAC | TGTCCACAATCTTTTGCAGCCT | GOOD | BAD | BAD | NR | BAD | - | - | - | 1.05 +/- 0 | |
| EMR3 | short | EMR3_cass_S_1_f | EMR3_cass_S_r | AGACTGCATTCTGGGAATGAACAA | TGTCCACAATCTTTTGCAGCCT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.02 +/- 0 | |
| GH1 | short | GH1_alt3_S_1_f | GH1_alt3_S_r | TTTGACAACGCTATGCTCCGC | AGAGGGAGGTCTGGGGGTTAA | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.04 +/- 0 | |
| GH1 | short | GH1_alt3_S_f | GH1_alt3_S_r | TTTTGACAACGCTATGCTCCGC | AGAGGGAGGTCTGGGGGTTAA | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.02 +/- 0 | |
| GPR18 | long | rs.GPR18.F1 | GPR18_cass_L_r | TCGACAGAAGTGGAAGTGCTGA | CTCTACTTCAGTGGTTCACAGAAAGC | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.05 +/- 0 | |
| GPR18 | long | GPR18_cass_L_f | GPR18_cass_L_1_r | TTCGACAGAAGTGGAAGTGCTG | ACTTCAGTGGTTCACAGAAAGCA | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.48 +/- 0 | |
| KCNJ16 | long | KCNJ16_cass_L_1_f | KCNJ16_cass_S_1_r | TCAGTCACGCAGTTGAGGTCAG | AGGCCAAAGGATTTCCCAGC | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.61 +/- 0 | |
| KCNJ16 | long | KCNJ16_cass_L_f | KCNJ16_cass_S_r | AGTCACGCAGTTGAGGTCAGG | ATAGGCCAAAGGATTTCCCAGC | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.54 +/- 0 | |
| KCNRG | short | KCNRG_cass_S_f | KCNRG_cass_S_r | ACTGATAACCAAACTGGAGTCAGGTA | AGCCGAGGACATTTGTTCCGTT | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.49 +/- 0 | |
| KCNRG | short | KCNRG_cass_S_f | KCNRG_cass_S_1_r | ACTGATAACCAAACTGGAGTCAGGTA | AAGCCGAGGACATTTGTTCCGT | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.53 +/- 0 | |
| KLRC1 | long | KLRC1_cass_L_f | KLRC1_cass_L_r | GGCCTCTGTGGTAACGATAGTTGT | TGACGTGCTTTCTGAGTTCTTGT | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.47 +/- 0 | |
| KLRC1 | long | KLRC1_cass_L_1_f | KLRC1_cass_L_1_r | TCTTAATGGCCTCTGTGGTAACGA | TCAGGGAAGAATTGTTGTGCCTC | GOOD | BAD | GOOD | NR | BAD | - | - | - | 1.48 +/- 0 | |
| KLRC1 | short | KLRC1_cass_S_1_f | KLRC1_cass_S_r | AGAAGCTCATTGTTGGGATCCTGG | ACAATGGCCACAATGACGTGAG | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.03 +/- 0 | |
| KLRC1 | short | KLRC1_cass_S_f | KLRC1_cass_S_r | AAGCTCATTGTTGGGATCCTGG | ACAATGGCCACAATGACGTGAG | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.03 +/- 0 | |
| LOC653188 | short | LOC653188_alt5_S_f | LOC653188_alt5_S_r | GGCATGTGAGCACATAATGGCA | CTGGTAATTCACCAGCCCTGG | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.04 +/- 0 | |
| LOC653188 | short | LOC653188_alt5_L_f | LOC653188_alt5_S_r | TGGGCATGTGAGCACATAATGG | CTGGTAATTCACCAGCCCTGG | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.45 +/- 0 | |
| MAP2 | short | MAP2_cass_S_f | MAP2_cass_L_r | GCCTAAAGGGGGGCAGGTAC | GAGCCACATTTGGATGTCACATGG | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.38 +/- 0 | |
| MAP2 | short | MAP2_cass_S_f | MAP2_cass_S_r | GCCTAAAGGGGGGCAGGTAC | TGTGGCGGATGTTCTTCAGAGA | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.38 +/- 0 | |
| MRVI1 | MRVI1_cass_S_f | MRVI1_cass_S_1_r | CATGCCCCACATTCCCGAGG | GGGCTCCACAGGCTTTAAGG | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.5 +/- 0 | ||
| MRVI1 | long | MRVI1_cass_L_1_f | MRVI1_cass_L_r | CCATGCCCCACATTCCCGAG | CTGCAAGATACTCCTGCGGC | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.41 +/- 0 | |
| MRVI1 | long | MRVI1_cass_L_3_f | MRVI1_cass_L_1_r | AGAATAACCTGCCTCTTCGGCTG | GGTGACAGTGCGAGACCCTG | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.42 +/- 0 | |
| MRVI1 | long | MRVI1_cass_L_f | MRVI1_cass_L_r | CCCACATTCCCGAGGACGAG | CTGCAAGATACTCCTGCGGC | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.49 +/- 0 | |
| MRVI1 | long | MRVI1_cass_L_2_f | MRVI1_cass_L_1_r | AGAATAACCTGCCTCTTCGGCT | GGTGACAGTGCGAGACCCTG | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.45 +/- 0 | |
| MRVI1 | short | MRVI1_cass_S_1_f | MRVI1_cass_S_r | CACATTCCCGAGGACGAGGA | GTGGGGACTGTGAACTTTGGC | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.4 +/- 0 | |
| MRVI1 | short | MRVI1_cass_S_3_f | MRVI1_cass_S_1_r | ATCCTAGAATAACCTGCCTCTTCGG | GGGCTCCACAGGCTTTAAGG | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.21 +/- 0 | |
| MRVI1 | short | MRVI1_cass_S_2_f | MRVI1_cass_S_1_r | TCCTAGAATAACCTGCCTCTTCGG | GGGCTCCACAGGCTTTAAGG | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.53 +/- 0 | |
| NRXN2 | long | NRXN2_cass_L_f | NRXN2_cass_L_1_r | TGTCAACCTCGACTGCCTGC | CCGTGTGCCACTCATTGTCATT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.05 +/- 0 | |
| NRXN2 | long | NRXN2_cass_L_f | NRXN2_cass_L_r | TGTCAACCTCGACTGCCTGC | ACCGTGTGCCACTCATTGTCAT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.1 +/- 0 | |
| NRXN2 | short | NRXN2_cass_S_1_f | NRXN2_cass_S_1_r | CCTGACGTTAAACAGCGAAGGC | TGTGTGACAGGTCGTAGCAGAA | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.5 +/- 0 | |
| NRXN2 | short | NRXN2_cass_S_1_f | NRXN2_cass_S_2_r | CCTGACGTTAAACAGCGAAGGC | TTGTGTGACAGGTCGTAGCAGA | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.49 +/- 0 | |
| OTOF | long | OTOF_alt3_L_f | OTOF_alt3_L_r | AACACCACGGTCAGGCTTCT | TCTGGCTCCATAGTCACCACAA | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.42 +/- 0 | |
| OTOF | long | OTOF_alt3_L_f | OTOF_alt3_L_1_r | AACACCACGGTCAGGCTTCT | TCTGGCTCCATAGTCACCACA | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.3 +/- 0 | |
| PTCH1 | short | PTCH1_alt5_S_f | PTCH1_alt5_S_r | GACCCGCAGCGACAAGGG | TCTCTGAAACTTCGCTCTCAGCC | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.43 +/- 0 | |
| PTCH1 | short | PTCH1_alt5_S_f | PTCH1_alt5_S_1_r | GACCCGCAGCGACAAGGG | AGTCTCTGAAACTTCGCTCTCAGC | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.43 +/- 0 | |
| RGS13 | long | RGS13_cass_S_f | RGS13_cass_L_r | GGAATGTTTCCACATATTATACCACCAAC | GAGAAGAGAAAAGGCAGCGTTAGA | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.42 +/- 0 | |
| RGS13 | long | RGS13_cass_L_f | RGS13_cass_L_r | TGGAATGTTTCCACATATTATACCACCA | GAGAAGAGAAAAGGCAGCGTTAGA | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.04 +/- 0 | |
| RGS13 | short | RGS13_cass_S_1_f | RGS13_cass_S_r | GTTTCCACATATTATACCACCAACATGAG | CAATTCCGCCTGCTCATTTTTCAA | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.39 +/- 0 | |
| RGS13 | short | RGS13_cass_S_f | RGS13_cass_S_r | GGAATGTTTCCACATATTATACCACCAAC | CAATTCCGCCTGCTCATTTTTCAA | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.13 +/- 0 | |
| RIMS2 | long | RIMS2_cass_L_f | RIMS2_cass_S_1_r | ACCATCATCACAGGGATGGCA | AGGAGAGGCCGTTGTTCTGTT | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.04 +/- 0 | |
| RIMS2 | long | RIMS2_cass_L_f | RIMS2_cass_S_r | ACCATCATCACAGGGATGGCA | AAGGAGAGGCCGTTGTTCTGTT | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.5 +/- 0 | |
| SLC6A20 | short | SLC6A20_cass_S_1_f | SLC6A20_cass_S_r | CGCTGTGCCTCCTCCTGG | GCCAGCTGCTCTATCTTGCC | BAD | GOOD | GOOD | NR | BAD | - | - | - | 1.56 +/- 0 | |
| SLC6A20 | short | SLC6A20_cass_S_f | SLC6A20_cass_S_r | GCGCTGTGCCTCCTCCTG | GCCAGCTGCTCTATCTTGCC | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.52 +/- 0 | |
| SPAG6 | long | SPAG6_cass_L_f | SPAG6_cass_S_r | CAGTTGTTACCCCGAGGAAATAGTGA | TTGATAGCTGTCCACCCTCTGC | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.46 +/- 0 | |
| SPAG6 | long | SPAG6_cass_L_f | SPAG6_cass_S_1_r | CAGTTGTTACCCCGAGGAAATAGTGA | TGATAGCTGTCCACCCTCTGC | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.79 +/- 0 | |
| SPAG6 | short | SPAG6_cass_S_f | SPAG6_cass_S_r | TGTGGTTGGACAGTTCAGTAAGGTAT | TTGATAGCTGTCCACCCTCTGC | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.51 +/- 0 | |
| SPAG6 | short | SPAG6_cass_S_f | SPAG6_cass_S_1_r | TGTGGTTGGACAGTTCAGTAAGGTAT | TGATAGCTGTCCACCCTCTGC | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.53 +/- 0 | |
| TPO | short | TPO_cass_S_f | TPO_cass_S_r | ACGGGTATGAGCTCCAAGGC | GGAGCCTCCCGGAGTCTTT | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.3 +/- 0 | |
| TPO | short | TPO_cass_S_1_f | TPO_cass_S_r | GAGCAGCTCACTTGCACCCA | GGAGCCTCCCGGAGTCTTT | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.53 +/- 0 | |
| TRIM55 | long | TRIM55_cass_L_f | TRIM55_cass_L_r | TGCCTAGCGCTTTTGGCTTTTC | AGGGGAGGAGCCTCAAATCCA | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.1 +/- 0 | |
| TRIM55 | long | TRIM55_cass_L_1_f | TRIM55_cass_L_r | GCCTAGCGCTTTTGGCTTTTCT | AGGGGAGGAGCCTCAAATCCA | BAD | GOOD | BAD | NR | BAD | - | - | - | 1.59 +/- 0 | |
| TRIM55 | short | TRIM55_cass_S_f | TRIM55_cass_S_1_r | GCACCTGCAGCTACTTCTCAGAT | CTCCACTGCCACTCGCTGGA | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.16 +/- 0 | |
| TRIM55 | short | TRIM55_cass_S_f | TRIM55_cass_S_r | GCACCTGCAGCTACTTCTCAGAT | TCCACTGCCACTCGCTGGA | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.1 +/- 0 | |
| TRPM3 | short | TRPM3_cass_L_f | TRPM3_cass_S_1_r | GCTGCAACTACACGCGCAA | CGCCTCAAGGGAATATCATCCCT | GOOD | BAD | BAD | NR | BAD | - | - | - | 1.21 +/- 0.14 | |
| TRPM3 | short | TRPM3_cass_S_2_f | TRPM3_cass_S_1_r | GGGCTTATCGCTGCAACTACAC | CGCCTCAAGGGAATATCATCCCT | GOOD | BAD | BAD | NR | BAD | - | - | - | 1.22 +/- 0 | |
| VSIG4 | long | VSIG4_cass_S_f | VSIG4_cass_L_r | TGATGGCAACCAAGTCGTGAGA | ATAACCGCTGCCAGTTGTCACT | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.19 +/- 0 | |
| VSIG4 | long | VSIG4_cass_S_1_f | VSIG4_cass_L_r | ATGGCAACCAAGTCGTGAGAGA | ATAACCGCTGCCAGTTGTCACT | GOOD | BAD | GOOD | NR | BAD | - | - | - | 1.51 +/- 0 | |
| VSIG4 | short | VSIG4_cass_S_f | VSIG4_cass_S_r | TGATGGCAACCAAGTCGTGAGA | CTTGAGTAGCTTTGAGGAGTGTTTCT | GOOD | BAD | GOOD | NR | BAD | - | - | - | 1.57 +/- 0 | |
| VSIG4 | short | VSIG4_cass_S_1_f | VSIG4_cass_S_r | ATGGCAACCAAGTCGTGAGAGA | CTTGAGTAGCTTTGAGGAGTGTTTCT | GOOD | GOOD | GOOD | NR | GOOD | - | - | - | 1.7 +/- 0 | |
| ZNFN1A3 | short | ZNFN1A3_cass_S_f | ZNFN1A3_cass_S_r | CTGCAACTATGCATGCCAAAGAAGA | GCCTCCGCACTTGCAGAA | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.01 +/- 0 | |
| ZNFN1A3 | short | ZNFN1A3_cass_S_1_f | ZNFN1A3_cass_S_r | TGCAACTATGCATGCCAAAGAAGA | GCCTCCGCACTTGCAGAA | GOOD | GOOD | BAD | NR | BAD | - | - | - | 1.02 +/- 0 | |
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