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Supplementary data
Tumor microenvironment associated modifications of alternative splicing
Splicing factor knockdown
Validation
XAB2
QPCR Primer Pair List
: primer pair list
| Gene | Forward | Reverse | |
|---|---|---|---|
| VIEW | HNRNPA1 | HNRNPA1_G_f | HNRNPA1_G_r |
| VIEW | PSMC4 | PSMC4_G_1_f | PSMC4_G_1_r |
| VIEW | RNPC3 | RNPC3_G_f | RNPC3_G_r |
| VIEW | SDHA | SDHA_G_f | SDHA_G_r |
| VIEW | U1SNRNPBP | U1SNRNPBP_G_1_f | U1SNRNPBP_G_1_r |
| VIEW | WBP4 | WBP4_G_f | WBP4_G_1_r |
| VIEW | XAB2 | XAB2.G1.F1 | XAB2.G1.R1 |
| VIEW | ZCCHC8 | ZCCHC8_G_f | ZCCHC8_G_r |
| VIEW | ZRSR2 | ZRSR2_G_f | ZRSR2_G_1_r |
current - show all
Page 1 of 1, records 1 to 9 of 9
QPCR Relative Expression Quantification (N1-3)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | HNRPA1__G_1_s;SKOV3ip1 |
| 2 | HNRPA1__G_s;SKOV3ip1 |
| 3 | KITLG_84_L_2_s;SKOV3ip1 |
| 4 | RNPC3__G_1_s;SKOV3ip1 |
| 5 | RNPC3__G_s;SKOV3ip1 |
| 6 | SKOV3ip1;Lipofectamine 2000 |
| 7 | U1SNRNPBP__G_1_s;SKOV3ip1 |
| 8 | U1SNRNPBP__G_s;SKOV3ip1 |
| 9 | WBP4__G_1_s;SKOV3ip1 |
| 10 | WBP4__G_s;SKOV3ip1 |
| 11 | XAB2__G_1_s;SKOV3ip1 |
| 12 | XAB2__G_s;SKOV3ip1 |
| 13 | ZCCHC8__G_1_s;SKOV3ip1 |
| 14 | ZCCHC8__G_s;SKOV3ip1 |
| 15 | ZRSR2__G_1_s;SKOV3ip1 |
| 16 | ZRSR2__G_s;SKOV3ip1 |
current - show all
Page 1 of 1, records 1 to 16 of 16
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 | S11 | p11 | S12 | p12 | S13 | p13 | S14 | p14 | S15 | p15 | S16 | p16 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HNRNPA1_G_f HNRNPA1_G_r | 1.449 | 0.486 | 0.961 | 1.000 | ||||||||||||||||||||||||||||
| RNPC3_G_f RNPC3_G_r | 1.074 | 4.757 | 3.171 | 1.000 | ||||||||||||||||||||||||||||
| U1SNRNPBP_G_1_f U1SNRNPBP_G_1_r | 1.000 | 3.160 | ||||||||||||||||||||||||||||||
| WBP4_G_f WBP4_G_1_r | 1.048 | 1.000 | 3.021 | 3.986 | ||||||||||||||||||||||||||||
| XAB2.G1.F1 XAB2.G1.R1 | 0.869 | 1.000 | 0.147 | 0.349 | ||||||||||||||||||||||||||||
| ZCCHC8_G_f ZCCHC8_G_r | 1.104 | 1.000 | 3.227 | 2.713 | ||||||||||||||||||||||||||||
| ZRSR2_G_f ZRSR2_G_1_r | 0.800 | 1.000 | 3.340 | 3.458 |
current - show all
Page 1 of 1, records 1 to 7 of 7
QPCR Relative Expression Quantification (N1)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | HNRPA1__G_1_s;SKOV3ip1 |
| 2 | HNRPA1__G_s;SKOV3ip1 |
| 3 | KITLG_84_L_2_s;SKOV3ip1 |
| 4 | RNPC3__G_1_s;SKOV3ip1 |
| 5 | RNPC3__G_s;SKOV3ip1 |
| 6 | SKOV3ip1;Lipofectamine 2000 |
| 7 | U1SNRNPBP__G_1_s;SKOV3ip1 |
| 8 | U1SNRNPBP__G_s;SKOV3ip1 |
| 9 | WBP4__G_1_s;SKOV3ip1 |
| 10 | WBP4__G_s;SKOV3ip1 |
| 11 | XAB2__G_1_s;SKOV3ip1 |
| 12 | XAB2__G_s;SKOV3ip1 |
| 13 | ZCCHC8__G_1_s;SKOV3ip1 |
| 14 | ZCCHC8__G_s;SKOV3ip1 |
| 15 | ZRSR2__G_1_s;SKOV3ip1 |
| 16 | ZRSR2__G_s;SKOV3ip1 |
current - show all
Page 1 of 1, records 1 to 16 of 16
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 | S11 | p11 | S12 | p12 | S13 | p13 | S14 | p14 | S15 | p15 | S16 | p16 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HNRNPA1_G_f HNRNPA1_G_r | 1.449 | 0.486 | 0.961 | 1.000 | ||||||||||||||||||||||||||||
| RNPC3_G_f RNPC3_G_r | 1.074 | 4.757 | 3.171 | 1.000 | ||||||||||||||||||||||||||||
| U1SNRNPBP_G_1_f U1SNRNPBP_G_1_r | 1.000 | 3.160 | ||||||||||||||||||||||||||||||
| WBP4_G_f WBP4_G_1_r | 1.048 | 1.000 | 3.021 | 3.986 | ||||||||||||||||||||||||||||
| XAB2.G1.F1 XAB2.G1.R1 | 0.869 | 1.000 | 0.147 | 0.349 | ||||||||||||||||||||||||||||
| ZCCHC8_G_f ZCCHC8_G_r | 1.104 | 1.000 | 3.227 | 2.713 | ||||||||||||||||||||||||||||
| ZRSR2_G_f ZRSR2_G_1_r | 0.800 | 1.000 | 3.340 | 3.458 |
current - show all
Page 1 of 1, records 1 to 7 of 7
QPCR Relative Expression Quantification (N2)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | HNRPA1__G_1_s;SKOV3ip1 |
| 2 | HNRPA1__G_s;SKOV3ip1 |
| 3 | KITLG_84_L_2_s;SKOV3ip1 |
| 4 | RNPC3__G_1_s;SKOV3ip1 |
| 5 | RNPC3__G_s;SKOV3ip1 |
| 6 | SKOV3ip1;Lipofectamine 2000 |
| 7 | U1SNRNPBP__G_1_s;SKOV3ip1 |
| 8 | U1SNRNPBP__G_s;SKOV3ip1 |
| 9 | WBP4__G_1_s;SKOV3ip1 |
| 10 | WBP4__G_s;SKOV3ip1 |
| 11 | XAB2__G_1_s;SKOV3ip1 |
| 12 | XAB2__G_s;SKOV3ip1 |
| 13 | ZCCHC8__G_1_s;SKOV3ip1 |
| 14 | ZCCHC8__G_s;SKOV3ip1 |
| 15 | ZRSR2__G_1_s;SKOV3ip1 |
| 16 | ZRSR2__G_s;SKOV3ip1 |
current - show all
Page 1 of 1, records 1 to 16 of 16
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 | S11 | p11 | S12 | p12 | S13 | p13 | S14 | p14 | S15 | p15 | S16 | p16 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HNRNPA1_G_f HNRNPA1_G_r | 1.449 | 0.486 | 0.961 | 1.000 | ||||||||||||||||||||||||||||
| RNPC3_G_f RNPC3_G_r | 1.074 | 4.757 | 3.171 | 1.000 | ||||||||||||||||||||||||||||
| U1SNRNPBP_G_1_f U1SNRNPBP_G_1_r | 1.000 | 3.160 | ||||||||||||||||||||||||||||||
| WBP4_G_f WBP4_G_1_r | 1.048 | 1.000 | 3.021 | 3.986 | ||||||||||||||||||||||||||||
| XAB2.G1.F1 XAB2.G1.R1 | 0.869 | 1.000 | 0.147 | 0.349 | ||||||||||||||||||||||||||||
| ZCCHC8_G_f ZCCHC8_G_r | 1.104 | 1.000 | 3.227 | 2.713 | ||||||||||||||||||||||||||||
| ZRSR2_G_f ZRSR2_G_1_r | 0.800 | 1.000 | 3.340 | 3.458 |
current - show all
Page 1 of 1, records 1 to 7 of 7
QPCR Relative Expression Quantification (N3)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | HNRPA1__G_1_s;SKOV3ip1 |
| 2 | HNRPA1__G_s;SKOV3ip1 |
| 3 | KITLG_84_L_2_s;SKOV3ip1 |
| 4 | RNPC3__G_1_s;SKOV3ip1 |
| 5 | RNPC3__G_s;SKOV3ip1 |
| 6 | SKOV3ip1;Lipofectamine 2000 |
| 7 | U1SNRNPBP__G_1_s;SKOV3ip1 |
| 8 | U1SNRNPBP__G_s;SKOV3ip1 |
| 9 | WBP4__G_1_s;SKOV3ip1 |
| 10 | WBP4__G_s;SKOV3ip1 |
| 11 | XAB2__G_1_s;SKOV3ip1 |
| 12 | XAB2__G_s;SKOV3ip1 |
| 13 | ZCCHC8__G_1_s;SKOV3ip1 |
| 14 | ZCCHC8__G_s;SKOV3ip1 |
| 15 | ZRSR2__G_1_s;SKOV3ip1 |
| 16 | ZRSR2__G_s;SKOV3ip1 |
current - show all
Page 1 of 1, records 1 to 16 of 16
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 | S11 | p11 | S12 | p12 | S13 | p13 | S14 | p14 | S15 | p15 | S16 | p16 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HNRNPA1_G_f HNRNPA1_G_r | 1.449 | 0.486 | 0.961 | 1.000 | ||||||||||||||||||||||||||||
| RNPC3_G_f RNPC3_G_r | 1.074 | 4.757 | 3.171 | 1.000 | ||||||||||||||||||||||||||||
| U1SNRNPBP_G_1_f U1SNRNPBP_G_1_r | 1.000 | 3.160 | ||||||||||||||||||||||||||||||
| WBP4_G_f WBP4_G_1_r | 1.048 | 1.000 | 3.021 | 3.986 | ||||||||||||||||||||||||||||
| XAB2.G1.F1 XAB2.G1.R1 | 0.869 | 1.000 | 0.147 | 0.349 | ||||||||||||||||||||||||||||
| ZCCHC8_G_f ZCCHC8_G_r | 1.104 | 1.000 | 3.227 | 2.713 | ||||||||||||||||||||||||||||
| ZRSR2_G_f ZRSR2_G_1_r | 0.800 | 1.000 | 3.340 | 3.458 |
current - show all
Page 1 of 1, records 1 to 7 of 7
QPCR Relative Expression Quantification (N1-3)
: Systematic Outlier Filtering
0 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
Click here to view
0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
Click here to view
QPCR Relative Expression Quantification (N1)
: Systematic Outlier Filtering
0 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
Click here to view
0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
Click here to view
QPCR Relative Expression Quantification (N2)
: Systematic Outlier Filtering
0 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
Click here to view
0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
Click here to view
QPCR Relative Expression Quantification (N3)
: Systematic Outlier Filtering
0 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
Click here to view
0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
Click here to view
QPCR Reference Gene Page (N1-3)
: .
QPCR Reference Gene Page (N1)
: .
QPCR Reference Gene Page (N2)
: .
QPCR Reference Gene Page (N3)
: .
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