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Supplementary data
Tumor microenvironment associated modifications of alternative splicing
Splicing factor knockdown
Validation
ACIN1 EWSR1
QPCR Primer Pair List
: primer pair list
QPCR Relative Expression Quantification (N1-3)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | CASC3_RBP_G_1_s;SKOV3ip1 |
| 2 | CASC3_RBP_G_s;SKOV3ip1 |
| 3 | KITLG_84_L_2_s;SKOV3ip1 |
| 4 | SI_SFRS5_global_1;SKOV3ip1 |
| 5 | SI_SFRS5_global_2;SKOV3ip1 |
| 6 | SKOV3ip1;Lipofectamine 2000 |
| 7 | siEWSR1_global_1;SKOV3ip1 |
| 8 | siEWSR1_global_2;SKOV3ip1 |
| 9 | si_ACIN1_global_1;SKOV3ip1 |
| 10 | si_ACIN1_global_2;SKOV3ip1 |
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Page 1 of 1, records 1 to 10 of 10
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACIN1_G_3_f ACIN1_G_2_r | 1.295 | 1.000 | 0.363 | 0.329 | ||||||||||||||||
| CASC3_G_f CASC3_G_r | 1.932 | 1.048 | 1.588 | 1.000 | ||||||||||||||||
| EWSR1_G_1_f EWSR1_G_r | 1.812 | 1.000 | 0.408 | 0.446 | ||||||||||||||||
| SFRS5_G_1_f SFRS5_G_r | 1.572 | 0.754 | 0.242 | 1.000 |
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Page 1 of 1, records 1 to 4 of 4
QPCR Relative Expression Quantification (N1)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | CASC3_RBP_G_1_s;SKOV3ip1 |
| 2 | CASC3_RBP_G_s;SKOV3ip1 |
| 3 | KITLG_84_L_2_s;SKOV3ip1 |
| 4 | SI_SFRS5_global_1;SKOV3ip1 |
| 5 | SI_SFRS5_global_2;SKOV3ip1 |
| 6 | SKOV3ip1;Lipofectamine 2000 |
| 7 | siEWSR1_global_1;SKOV3ip1 |
| 8 | siEWSR1_global_2;SKOV3ip1 |
| 9 | si_ACIN1_global_1;SKOV3ip1 |
| 10 | si_ACIN1_global_2;SKOV3ip1 |
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Page 1 of 1, records 1 to 10 of 10
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACIN1_G_3_f ACIN1_G_2_r | 1.295 | 1.000 | 0.363 | 0.329 | ||||||||||||||||
| CASC3_G_f CASC3_G_r | 1.932 | 1.048 | 1.588 | 1.000 | ||||||||||||||||
| EWSR1_G_1_f EWSR1_G_r | 1.812 | 1.000 | 0.408 | 0.446 | ||||||||||||||||
| SFRS5_G_1_f SFRS5_G_r | 1.572 | 0.754 | 0.242 | 1.000 |
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Page 1 of 1, records 1 to 4 of 4
QPCR Relative Expression Quantification (N2)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | CASC3_RBP_G_1_s;SKOV3ip1 |
| 2 | CASC3_RBP_G_s;SKOV3ip1 |
| 3 | KITLG_84_L_2_s;SKOV3ip1 |
| 4 | SI_SFRS5_global_1;SKOV3ip1 |
| 5 | SI_SFRS5_global_2;SKOV3ip1 |
| 6 | SKOV3ip1;Lipofectamine 2000 |
| 7 | siEWSR1_global_1;SKOV3ip1 |
| 8 | siEWSR1_global_2;SKOV3ip1 |
| 9 | si_ACIN1_global_1;SKOV3ip1 |
| 10 | si_ACIN1_global_2;SKOV3ip1 |
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Page 1 of 1, records 1 to 10 of 10
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACIN1_G_3_f ACIN1_G_2_r | 1.295 | 1.000 | 0.363 | 0.329 | ||||||||||||||||
| CASC3_G_f CASC3_G_r | 1.932 | 1.048 | 1.588 | 1.000 | ||||||||||||||||
| EWSR1_G_1_f EWSR1_G_r | 1.812 | 1.000 | 0.408 | 0.446 | ||||||||||||||||
| SFRS5_G_1_f SFRS5_G_r | 1.572 | 0.754 | 0.242 | 1.000 |
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Page 1 of 1, records 1 to 4 of 4
QPCR Relative Expression Quantification (N3)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | CASC3_RBP_G_1_s;SKOV3ip1 |
| 2 | CASC3_RBP_G_s;SKOV3ip1 |
| 3 | KITLG_84_L_2_s;SKOV3ip1 |
| 4 | SI_SFRS5_global_1;SKOV3ip1 |
| 5 | SI_SFRS5_global_2;SKOV3ip1 |
| 6 | SKOV3ip1;Lipofectamine 2000 |
| 7 | siEWSR1_global_1;SKOV3ip1 |
| 8 | siEWSR1_global_2;SKOV3ip1 |
| 9 | si_ACIN1_global_1;SKOV3ip1 |
| 10 | si_ACIN1_global_2;SKOV3ip1 |
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Page 1 of 1, records 1 to 10 of 10
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACIN1_G_3_f ACIN1_G_2_r | 1.295 | 1.000 | 0.363 | 0.329 | ||||||||||||||||
| CASC3_G_f CASC3_G_r | 1.932 | 1.048 | 1.588 | 1.000 | ||||||||||||||||
| EWSR1_G_1_f EWSR1_G_r | 1.812 | 1.000 | 0.408 | 0.446 | ||||||||||||||||
| SFRS5_G_1_f SFRS5_G_r | 1.572 | 0.754 | 0.242 | 1.000 |
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Page 1 of 1, records 1 to 4 of 4
QPCR Relative Expression Quantification (N1-3)
: Systematic Outlier Filtering
0 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
Click here to view
0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
Click here to view
QPCR Relative Expression Quantification (N1)
: Systematic Outlier Filtering
0 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
Click here to view
0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
Click here to view
QPCR Relative Expression Quantification (N2)
: Systematic Outlier Filtering
0 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
Click here to view
0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
Click here to view
QPCR Relative Expression Quantification (N3)
: Systematic Outlier Filtering
0 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
Click here to view
0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
Click here to view
QPCR Reference Gene Page (N1-3)
: .
QPCR Reference Gene Page (N1)
: .
QPCR Reference Gene Page (N2)
: .
QPCR Reference Gene Page (N3)
: .
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