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2. Breast Validation Screen 
Gene primer pair list : Primer pairs
Forward Reverse Occurences View
SCN5A.F9 SCN5A.R10 51 View
Transcript map : SCN5A
Exon
Length: 142
Transcript: SCN5A.aAug05
Exon
Length: 325
Transcript: SCN5A.aAug05
Exon
Length: 119
Transcript: SCN5A.aAug05
Exon
Length: 90
Transcript: SCN5A.aAug05
Exon
Length: 129
Transcript: SCN5A.aAug05
Exon
Length: 92
Transcript: SCN5A.aAug05
Exon
Length: 231
Transcript: SCN5A.aAug05
Exon
Length: 64
Transcript: SCN5A.aAug05
Exon
Length: 142
Transcript: SCN5A.aAug05
Exon
Length: 198
Transcript: SCN5A.aAug05
Exon
Length: 180
Transcript: SCN5A.aAug05
Exon
Length: 372
Transcript: SCN5A.aAug05
Exon
Length: 133
Transcript: SCN5A.aAug05
Exon
Length: 239
Transcript: SCN5A.aAug05
Exon
Length: 174
Transcript: SCN5A.aAug05
Exon
Length: 351
Transcript: SCN5A.aAug05
Exon
Length: 441
Transcript: SCN5A.aAug05
Exon
Length: 162
Transcript: SCN5A.aAug05
Exon
Length: 121
Transcript: SCN5A.aAug05
Exon
Length: 155
Transcript: SCN5A.aAug05
Exon
Length: 174
Transcript: SCN5A.aAug05
Exon
Length: 123
Transcript: SCN5A.aAug05
Exon
Length: 282
Transcript: SCN5A.aAug05
Exon
Length: 54
Transcript: SCN5A.aAug05
Exon
Length: 138
Transcript: SCN5A.aAug05
Exon
Length: 105
Transcript: SCN5A.aAug05
Exon
Length: 271
Transcript: SCN5A.aAug05
Exon
Length: 3496
Transcript: SCN5A.aAug05
Intron
Length: 16171
Transcript: SCN5A.aAug05
Intron
Length: 2605
Transcript: SCN5A.aAug05
Intron
Length: 7821
Transcript: SCN5A.aAug05
Intron
Length: 1428
Transcript: SCN5A.aAug05
Intron
Length: 7008
Transcript: SCN5A.aAug05
Intron
Length: 3778
Transcript: SCN5A.aAug05
Intron
Length: 1519
Transcript: SCN5A.aAug05
Intron
Length: 1340
Transcript: SCN5A.aAug05
Intron
Length: 520
Transcript: SCN5A.aAug05
Intron
Length: 1042
Transcript: SCN5A.aAug05
Intron
Length: 645
Transcript: SCN5A.aAug05
Intron
Length: 4661
Transcript: SCN5A.aAug05
Intron
Length: 950
Transcript: SCN5A.aAug05
Intron
Length: 10155
Transcript: SCN5A.aAug05
Intron
Length: 1358
Transcript: SCN5A.aAug05
Intron
Length: 4319
Transcript: SCN5A.aAug05
Intron
Length: 1435
Transcript: SCN5A.aAug05
Intron
Length: 2552
Transcript: SCN5A.aAug05
Intron
Length: 1209
Transcript: SCN5A.aAug05
Intron
Length: 8714
Transcript: SCN5A.aAug05
Intron
Length: 3871
Transcript: SCN5A.aAug05
Intron
Length: 1986
Transcript: SCN5A.aAug05
Intron
Length: 2862
Transcript: SCN5A.aAug05
Intron
Length: 652
Transcript: SCN5A.aAug05
Intron
Length: 680
Transcript: SCN5A.aAug05
Intron
Length: 1106
Transcript: SCN5A.aAug05
Intron
Length: 2720
Transcript: SCN5A.aAug05
Exon
Length: 142
Transcript: SCN5A.bAug05
Exon
Length: 325
Transcript: SCN5A.bAug05
Exon
Length: 119
Transcript: SCN5A.bAug05
Exon
Length: 90
Transcript: SCN5A.bAug05
Exon
Length: 129
Transcript: SCN5A.bAug05
Exon
Length: 92
Transcript: SCN5A.bAug05
Exon
Length: 231
Transcript: SCN5A.bAug05
Exon
Length: 64
Transcript: SCN5A.bAug05
Exon
Length: 142
Transcript: SCN5A.bAug05
Exon
Length: 198
Transcript: SCN5A.bAug05
Exon
Length: 180
Transcript: SCN5A.bAug05
Exon
Length: 372
Transcript: SCN5A.bAug05
Exon
Length: 133
Transcript: SCN5A.bAug05
Exon
Length: 239
Transcript: SCN5A.bAug05
Exon
Length: 174
Transcript: SCN5A.bAug05
Exon
Length: 351
Transcript: SCN5A.bAug05
Exon
Length: 441
Transcript: SCN5A.bAug05
Exon
Length: 159
Transcript: SCN5A.bAug05
Exon
Length: 121
Transcript: SCN5A.bAug05
Exon
Length: 155
Transcript: SCN5A.bAug05
Exon
Length: 174
Transcript: SCN5A.bAug05
Exon
Length: 123
Transcript: SCN5A.bAug05
Exon
Length: 282
Transcript: SCN5A.bAug05
Exon
Length: 54
Transcript: SCN5A.bAug05
Exon
Length: 138
Transcript: SCN5A.bAug05
Exon
Length: 105
Transcript: SCN5A.bAug05
Exon
Length: 271
Transcript: SCN5A.bAug05
Exon
Length: 3496
Transcript: SCN5A.bAug05
Intron
Length: 16171
Transcript: SCN5A.bAug05
Intron
Length: 2605
Transcript: SCN5A.bAug05
Intron
Length: 7821
Transcript: SCN5A.bAug05
Intron
Length: 1428
Transcript: SCN5A.bAug05
Intron
Length: 7008
Transcript: SCN5A.bAug05
Intron
Length: 3778
Transcript: SCN5A.bAug05
Intron
Length: 1519
Transcript: SCN5A.bAug05
Intron
Length: 1340
Transcript: SCN5A.bAug05
Intron
Length: 520
Transcript: SCN5A.bAug05
Intron
Length: 1042
Transcript: SCN5A.bAug05
Intron
Length: 645
Transcript: SCN5A.bAug05
Intron
Length: 4661
Transcript: SCN5A.bAug05
Intron
Length: 950
Transcript: SCN5A.bAug05
Intron
Length: 10155
Transcript: SCN5A.bAug05
Intron
Length: 1358
Transcript: SCN5A.bAug05
Intron
Length: 4319
Transcript: SCN5A.bAug05
Intron
Length: 1438
Transcript: SCN5A.bAug05
Intron
Length: 2552
Transcript: SCN5A.bAug05
Intron
Length: 1209
Transcript: SCN5A.bAug05
Intron
Length: 8714
Transcript: SCN5A.bAug05
Intron
Length: 3871
Transcript: SCN5A.bAug05
Intron
Length: 1986
Transcript: SCN5A.bAug05
Intron
Length: 2862
Transcript: SCN5A.bAug05
Intron
Length: 652
Transcript: SCN5A.bAug05
Intron
Length: 680
Transcript: SCN5A.bAug05
Intron
Length: 1106
Transcript: SCN5A.bAug05
Intron
Length: 2720
Transcript: SCN5A.bAug05
Exon
Length: 315
Transcript: SCN5A.cAug05
Exon
Length: 119
Transcript: SCN5A.cAug05
Exon
Length: 90
Transcript: SCN5A.cAug05
Exon
Length: 129
Transcript: SCN5A.cAug05
Exon
Length: 92
Transcript: SCN5A.cAug05
Exon
Length: 231
Transcript: SCN5A.cAug05
Exon
Length: 64
Transcript: SCN5A.cAug05
Exon
Length: 142
Transcript: SCN5A.cAug05
Exon
Length: 198
Transcript: SCN5A.cAug05
Exon
Length: 180
Transcript: SCN5A.cAug05
Exon
Length: 372
Transcript: SCN5A.cAug05
Exon
Length: 133
Transcript: SCN5A.cAug05
Exon
Length: 239
Transcript: SCN5A.cAug05
Exon
Length: 174
Transcript: SCN5A.cAug05
Exon
Length: 351
Transcript: SCN5A.cAug05
Exon
Length: 441
Transcript: SCN5A.cAug05
Exon
Length: 162
Transcript: SCN5A.cAug05
Exon
Length: 121
Transcript: SCN5A.cAug05
Exon
Length: 155
Transcript: SCN5A.cAug05
Exon
Length: 174
Transcript: SCN5A.cAug05
Exon
Length: 123
Transcript: SCN5A.cAug05
Exon
Length: 282
Transcript: SCN5A.cAug05
Exon
Length: 54
Transcript: SCN5A.cAug05
Exon
Length: 138
Transcript: SCN5A.cAug05
Exon
Length: 105
Transcript: SCN5A.cAug05
Exon
Length: 271
Transcript: SCN5A.cAug05
Exon
Length: 2210
Transcript: SCN5A.cAug05
Intron
Length: 2605
Transcript: SCN5A.cAug05
Intron
Length: 7821
Transcript: SCN5A.cAug05
Intron
Length: 1428
Transcript: SCN5A.cAug05
Intron
Length: 6776
Transcript: SCN5A.cAug05
Intron
Length: 4010
Transcript: SCN5A.cAug05
Intron
Length: 1519
Transcript: SCN5A.cAug05
Intron
Length: 1340
Transcript: SCN5A.cAug05
Intron
Length: 520
Transcript: SCN5A.cAug05
Intron
Length: 1042
Transcript: SCN5A.cAug05
Intron
Length: 645
Transcript: SCN5A.cAug05
Intron
Length: 4661
Transcript: SCN5A.cAug05
Intron
Length: 950
Transcript: SCN5A.cAug05
Intron
Length: 10155
Transcript: SCN5A.cAug05
Intron
Length: 1358
Transcript: SCN5A.cAug05
Intron
Length: 4319
Transcript: SCN5A.cAug05
Intron
Length: 1435
Transcript: SCN5A.cAug05
Intron
Length: 2552
Transcript: SCN5A.cAug05
Intron
Length: 1209
Transcript: SCN5A.cAug05
Intron
Length: 8714
Transcript: SCN5A.cAug05
Intron
Length: 3871
Transcript: SCN5A.cAug05
Intron
Length: 1986
Transcript: SCN5A.cAug05
Intron
Length: 2862
Transcript: SCN5A.cAug05
Intron
Length: 652
Transcript: SCN5A.cAug05
Intron
Length: 680
Transcript: SCN5A.cAug05
Intron
Length: 1106
Transcript: SCN5A.cAug05
Intron
Length: 2720
Transcript: SCN5A.cAug05
Reaction
Expected peak: 221
Associated peak: 233,234,235,236,237
Repeated 51 time
Molarity: 3.55M
Forward: SCN5A.F9
Reverse: SCN5A.R10
Exon
Length: 315
Transcript: SCN5A.dAug05
Exon
Length: 119
Transcript: SCN5A.dAug05
Exon
Length: 90
Transcript: SCN5A.dAug05
Exon
Length: 129
Transcript: SCN5A.dAug05
Exon
Length: 92
Transcript: SCN5A.dAug05
Exon
Length: 231
Transcript: SCN5A.dAug05
Exon
Length: 64
Transcript: SCN5A.dAug05
Exon
Length: 142
Transcript: SCN5A.dAug05
Exon
Length: 198
Transcript: SCN5A.dAug05
Exon
Length: 180
Transcript: SCN5A.dAug05
Exon
Length: 372
Transcript: SCN5A.dAug05
Exon
Length: 133
Transcript: SCN5A.dAug05
Exon
Length: 239
Transcript: SCN5A.dAug05
Exon
Length: 174
Transcript: SCN5A.dAug05
Exon
Length: 351
Transcript: SCN5A.dAug05
Exon
Length: 441
Transcript: SCN5A.dAug05
Exon
Length: 121
Transcript: SCN5A.dAug05
Exon
Length: 155
Transcript: SCN5A.dAug05
Exon
Length: 174
Transcript: SCN5A.dAug05
Exon
Length: 123
Transcript: SCN5A.dAug05
Exon
Length: 282
Transcript: SCN5A.dAug05
Exon
Length: 54
Transcript: SCN5A.dAug05
Exon
Length: 138
Transcript: SCN5A.dAug05
Exon
Length: 105
Transcript: SCN5A.dAug05
Exon
Length: 271
Transcript: SCN5A.dAug05
Exon
Length: 1591
Transcript: SCN5A.dAug05
Intron
Length: 2605
Transcript: SCN5A.dAug05
Intron
Length: 7821
Transcript: SCN5A.dAug05
Intron
Length: 1428
Transcript: SCN5A.dAug05
Intron
Length: 6776
Transcript: SCN5A.dAug05
Intron
Length: 4010
Transcript: SCN5A.dAug05
Intron
Length: 1519
Transcript: SCN5A.dAug05
Intron
Length: 1340
Transcript: SCN5A.dAug05
Intron
Length: 520
Transcript: SCN5A.dAug05
Intron
Length: 1042
Transcript: SCN5A.dAug05
Intron
Length: 645
Transcript: SCN5A.dAug05
Intron
Length: 4661
Transcript: SCN5A.dAug05
Intron
Length: 950
Transcript: SCN5A.dAug05
Intron
Length: 10155
Transcript: SCN5A.dAug05
Intron
Length: 1358
Transcript: SCN5A.dAug05
Intron
Length: 4319
Transcript: SCN5A.dAug05
Intron
Length: 4149
Transcript: SCN5A.dAug05
Intron
Length: 1209
Transcript: SCN5A.dAug05
Intron
Length: 8714
Transcript: SCN5A.dAug05
Intron
Length: 3871
Transcript: SCN5A.dAug05
Intron
Length: 1986
Transcript: SCN5A.dAug05
Intron
Length: 2862
Transcript: SCN5A.dAug05
Intron
Length: 652
Transcript: SCN5A.dAug05
Intron
Length: 680
Transcript: SCN5A.dAug05
Intron
Length: 1106
Transcript: SCN5A.dAug05
Intron
Length: 2720
Transcript: SCN5A.dAug05
Reaction
Expected peak: 221
Associated peak: 233,234,235,236,237
Repeated 51 time
Molarity: 3.55M
Forward: SCN5A.F9
Reverse: SCN5A.R10
Exon
Length: 76
Transcript: SCN5A.eAug05
Exon
Length: 180
Transcript: SCN5A.eAug05
Exon
Length: 372
Transcript: SCN5A.eAug05
Exon
Length: 133
Transcript: SCN5A.eAug05
Exon
Length: 239
Transcript: SCN5A.eAug05
Exon
Length: 174
Transcript: SCN5A.eAug05
Exon
Length: 351
Transcript: SCN5A.eAug05
Exon
Length: 441
Transcript: SCN5A.eAug05
Exon
Length: 159
Transcript: SCN5A.eAug05
Exon
Length: 121
Transcript: SCN5A.eAug05
Exon
Length: 155
Transcript: SCN5A.eAug05
Exon
Length: 174
Transcript: SCN5A.eAug05
Exon
Length: 123
Transcript: SCN5A.eAug05
Exon
Length: 282
Transcript: SCN5A.eAug05
Exon
Length: 138
Transcript: SCN5A.eAug05
Exon
Length: 105
Transcript: SCN5A.eAug05
Exon
Length: 271
Transcript: SCN5A.eAug05
Exon
Length: 3392
Transcript: SCN5A.eAug05
Intron
Length: 1042
Transcript: SCN5A.eAug05
Intron
Length: 645
Transcript: SCN5A.eAug05
Intron
Length: 4661
Transcript: SCN5A.eAug05
Intron
Length: 950
Transcript: SCN5A.eAug05
Intron
Length: 10155
Transcript: SCN5A.eAug05
Intron
Length: 1358
Transcript: SCN5A.eAug05
Intron
Length: 4319
Transcript: SCN5A.eAug05
Intron
Length: 1438
Transcript: SCN5A.eAug05
Intron
Length: 2552
Transcript: SCN5A.eAug05
Intron
Length: 1209
Transcript: SCN5A.eAug05
Intron
Length: 8714
Transcript: SCN5A.eAug05
Intron
Length: 3871
Transcript: SCN5A.eAug05
Intron
Length: 1986
Transcript: SCN5A.eAug05
Intron
Length: 3568
Transcript: SCN5A.eAug05
Intron
Length: 680
Transcript: SCN5A.eAug05
Intron
Length: 1106
Transcript: SCN5A.eAug05
Intron
Length: 2720
Transcript: SCN5A.eAug05
Exon
Length: 143
Transcript: SCN5A.fAug05
Exon
Length: 325
Transcript: SCN5A.fAug05
Exon
Length: 119
Transcript: SCN5A.fAug05
Exon
Length: 90
Transcript: SCN5A.fAug05
Exon
Length: 129
Transcript: SCN5A.fAug05
Exon
Length: 576
Transcript: SCN5A.fAug05
Intron
Length: 16171
Transcript: SCN5A.fAug05
Intron
Length: 2605
Transcript: SCN5A.fAug05
Intron
Length: 7821
Transcript: SCN5A.fAug05
Intron
Length: 1428
Transcript: SCN5A.fAug05
Intron
Length: 6239
Transcript: SCN5A.fAug05
Exon
Length: 186
Transcript: SCN5A.gAug05
Exon
Length: 514
Transcript: SCN5A.gAug05
Intron
Length: 2605
Transcript: SCN5A.gAug05
Exon
Length: 43
Transcript: SCN5A.hAug05
Exon
Length: 92
Transcript: SCN5A.hAug05
Exon
Length: 205
Transcript: SCN5A.hAug05
Intron
Length: 8333
Transcript: SCN5A.hAug05
Intron
Length: 4010
Transcript: SCN5A.hAug05
Reaction
Expected peak: 92
Associated peak: 99
Repeated 51 time
Molarity: 0.18M
Forward: SCN5A.F9
Reverse: SCN5A.R10
SCN5A.F9
AGCACGACCCTCCACCCT
SCN5A.R10
GACTCTGAAAGTTCGAAGAGCC

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