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2. Breast Validation Screen 
Transcript map : C11ORF4
Exon
Length: 464
Transcript: C11ORF4.aAug05
Exon
Length: 50
Transcript: C11ORF4.aAug05
Exon
Length: 226
Transcript: C11ORF4.aAug05
Exon
Length: 150
Transcript: C11ORF4.aAug05
Exon
Length: 129
Transcript: C11ORF4.aAug05
Exon
Length: 119
Transcript: C11ORF4.aAug05
Exon
Length: 464
Transcript: C11ORF4.aAug05
Intron
Length: 83
Transcript: C11ORF4.aAug05
Intron
Length: 706
Transcript: C11ORF4.aAug05
Intron
Length: 118
Transcript: C11ORF4.aAug05
Intron
Length: 125
Transcript: C11ORF4.aAug05
Intron
Length: 134
Transcript: C11ORF4.aAug05
Intron
Length: 315
Transcript: C11ORF4.aAug05
Reaction
Expected peak: 260
Associated peak: 273,274,275,276,277,278
Repeated 51 time
Molarity: 29.51M
Forward: C11ORF4.F13
Reverse: C11ORF4.R5
Reaction
Expected peak: 274
Associated peak: 284,285,286,287,288,289
Repeated 51 time
Molarity: 23.30M
Forward: C11ORF4.F3
Reverse: C11ORF4.R3
Reaction
Expected peak: 290
Associated peak: 266,267,268,269,270,274,275,295,296,297,298,299,300
Repeated 51 time
Molarity: 13.91M
Forward: C11ORF4.F1
Reverse: C11ORF4.R1
Reaction
Expected peak: 324
Associated peak: 349,350,351,352,353,354,355,356
Repeated 51 time
Molarity: 12.14M
Forward: C11ORF4.F13
Reverse: C11ORF4.R15
Reaction
Expected peak: 398
Associated peak: 434,435,436,437,438,439,440,441
Repeated 51 time
Molarity: 18.77M
Forward: C11ORF4.F2
Reverse: C11ORF4.R2
Reaction
Expected peak: 607
Associated peak: 633,639,644,645,647,649,650,652,653,655,656,658,659,660,661,662
Repeated 51 time
Molarity: 5.89M
Forward: C11ORF4.F1
Reverse: C11ORF4.R14
Exon
Length: 124
Transcript: C11ORF4.bAug05
Exon
Length: 372
Transcript: C11ORF4.bAug05
Exon
Length: 50
Transcript: C11ORF4.bAug05
Exon
Length: 226
Transcript: C11ORF4.bAug05
Exon
Length: 129
Transcript: C11ORF4.bAug05
Exon
Length: 119
Transcript: C11ORF4.bAug05
Exon
Length: 457
Transcript: C11ORF4.bAug05
Intron
Length: 1129
Transcript: C11ORF4.bAug05
Intron
Length: 83
Transcript: C11ORF4.bAug05
Intron
Length: 706
Transcript: C11ORF4.bAug05
Intron
Length: 393
Transcript: C11ORF4.bAug05
Intron
Length: 134
Transcript: C11ORF4.bAug05
Intron
Length: 315
Transcript: C11ORF4.bAug05
Reaction
Expected peak: 110
Associated peak: 120,121,122,123,124
Repeated 51 time
Molarity: 7.53M
Forward: C11ORF4.F13
Reverse: C11ORF4.R5
Reaction
Expected peak: 124
Associated peak: 134,135,136
Repeated 51 time
Molarity: 2.05M
Forward: C11ORF4.F3
Reverse: C11ORF4.R3
Reaction
Expected peak: 174
Associated peak: 192,193,194
Repeated 51 time
Molarity: 2.74M
Forward: C11ORF4.F13
Reverse: C11ORF4.R15
Reaction
Expected peak: 248
Associated peak: 261,262,263,264
Repeated 51 time
Molarity: 3.08M
Forward: C11ORF4.F2
Reverse: C11ORF4.R2
Reaction
Expected peak: 395
Associated peak: 433,434,435,436,437,438,439,440
Repeated 51 time
Molarity: 18.76M
Forward: C11ORF4.F6
Reverse: C11ORF4.R6
Reaction
Expected peak: 457
Associated peak: 477,479,480,481,482,483,484,488,497
Repeated 51 time
Molarity: 0.58M
Forward: C11ORF4.F1
Reverse: C11ORF4.R14
Exon
Length: 118
Transcript: C11ORF4.cAug05
Exon
Length: 372
Transcript: C11ORF4.cAug05
Exon
Length: 50
Transcript: C11ORF4.cAug05
Exon
Length: 226
Transcript: C11ORF4.cAug05
Exon
Length: 150
Transcript: C11ORF4.cAug05
Exon
Length: 129
Transcript: C11ORF4.cAug05
Exon
Length: 235
Transcript: C11ORF4.cAug05
Intron
Length: 902
Transcript: C11ORF4.cAug05
Intron
Length: 83
Transcript: C11ORF4.cAug05
Intron
Length: 706
Transcript: C11ORF4.cAug05
Intron
Length: 118
Transcript: C11ORF4.cAug05
Intron
Length: 125
Transcript: C11ORF4.cAug05
Intron
Length: 568
Transcript: C11ORF4.cAug05
Reaction
Expected peak: 260
Associated peak: 273,274,275,276,277,278
Repeated 51 time
Molarity: 29.51M
Forward: C11ORF4.F13
Reverse: C11ORF4.R5
Reaction
Expected peak: 274
Associated peak: 284,285,286,287,288,289
Repeated 51 time
Molarity: 23.30M
Forward: C11ORF4.F3
Reverse: C11ORF4.R3
Reaction
Expected peak: 290
Associated peak: 266,267,268,269,270,274,275,295,296,297,298,299,300
Repeated 51 time
Molarity: 13.91M
Forward: C11ORF4.F1
Reverse: C11ORF4.R1
Reaction
Expected peak: 398
Associated peak: 434,435,436,437,438,439,440,441
Repeated 51 time
Molarity: 18.77M
Forward: C11ORF4.F2
Reverse: C11ORF4.R2
Exon
Length: 816
Transcript: C11ORF4.dAug05
Exon
Length: 372
Transcript: C11ORF4.dAug05
Exon
Length: 50
Transcript: C11ORF4.dAug05
Exon
Length: 226
Transcript: C11ORF4.dAug05
Exon
Length: 404
Transcript: C11ORF4.dAug05
Exon
Length: 119
Transcript: C11ORF4.dAug05
Exon
Length: 74
Transcript: C11ORF4.dAug05
Intron
Length: 15848
Transcript: C11ORF4.dAug05
Intron
Length: 83
Transcript: C11ORF4.dAug05
Intron
Length: 706
Transcript: C11ORF4.dAug05
Intron
Length: 118
Transcript: C11ORF4.dAug05
Intron
Length: 134
Transcript: C11ORF4.dAug05
Intron
Length: 315
Transcript: C11ORF4.dAug05
Reaction
Expected peak: 290
Associated peak: 266,267,268,269,270,274,275,295,296,297,298,299,300
Repeated 51 time
Molarity: 13.91M
Forward: C11ORF4.F1
Reverse: C11ORF4.R1
Reaction
Expected peak: 385
Associated peak: 417,420,421,423,424,425,426,427,429,430,431,432,433
Repeated 51 time
Molarity: 0.60M
Forward: C11ORF4.F13
Reverse: C11ORF4.R5
Reaction
Expected peak: 399
Associated peak: None
Repeated 51 time
Molarity: 0.00M
Forward: C11ORF4.F3
Reverse: C11ORF4.R3
Reaction
Expected peak: 449
Associated peak: None
Repeated 51 time
Molarity: 0.00M
Forward: C11ORF4.F13
Reverse: C11ORF4.R15
Reaction
Expected peak: 523
Associated peak: None
Repeated 51 time
Molarity: 0.00M
Forward: C11ORF4.F2
Reverse: C11ORF4.R2
Reaction
Expected peak: 732
Associated peak: 659,660,661,662,668,722,723,733,744,800
Repeated 51 time
Molarity: 0.76M
Forward: C11ORF4.F1
Reverse: C11ORF4.R14
Exon
Length: 1400
Transcript: C11ORF4.eAug05
Exon
Length: 50
Transcript: C11ORF4.eAug05
Exon
Length: 344
Transcript: C11ORF4.eAug05
Intron
Length: 83
Transcript: C11ORF4.eAug05
Intron
Length: 706
Transcript: C11ORF4.eAug05
Reaction
Expected peak: 408
Associated peak: None
Repeated 51 time
Molarity: 0.00M
Forward: C11ORF4.F1
Reverse: C11ORF4.R1
Exon
Length: 565
Transcript: C11ORF4.fAug05
Exon
Length: 372
Transcript: C11ORF4.fAug05
Exon
Length: 50
Transcript: C11ORF4.fAug05
Exon
Length: 226
Transcript: C11ORF4.fAug05
Exon
Length: 26
Transcript: C11ORF4.fAug05
Intron
Length: 1129
Transcript: C11ORF4.fAug05
Intron
Length: 83
Transcript: C11ORF4.fAug05
Intron
Length: 706
Transcript: C11ORF4.fAug05
Intron
Length: 118
Transcript: C11ORF4.fAug05
Reaction
Expected peak: 290
Associated peak: 266,267,268,269,270,274,275,295,296,297,298,299,300
Repeated 51 time
Molarity: 13.91M
Forward: C11ORF4.F1
Reverse: C11ORF4.R1
Reaction
Expected peak: 395
Associated peak: 433,434,435,436,437,438,439,440
Repeated 51 time
Molarity: 18.76M
Forward: C11ORF4.F6
Reverse: C11ORF4.R6
Exon
Length: 72
Transcript: C11ORF4.gAug05
Exon
Length: 74
Transcript: C11ORF4.gAug05
Exon
Length: 372
Transcript: C11ORF4.gAug05
Exon
Length: 50
Transcript: C11ORF4.gAug05
Exon
Length: 96
Transcript: C11ORF4.gAug05
Intron
Length: 588
Transcript: C11ORF4.gAug05
Intron
Length: 10088
Transcript: C11ORF4.gAug05
Intron
Length: 83
Transcript: C11ORF4.gAug05
Intron
Length: 980
Transcript: C11ORF4.gAug05
Reaction
Expected peak: 134
Associated peak: 142,143,144,145
Repeated 51 time
Molarity: 0.78M
Forward: C11ORF4.F1
Reverse: C11ORF4.R1
Exon
Length: 21
Transcript: C11ORF4.hAug05
Exon
Length: 129
Transcript: C11ORF4.hAug05
Exon
Length: 119
Transcript: C11ORF4.hAug05
Exon
Length: 240
Transcript: C11ORF4.hAug05
Intron
Length: 125
Transcript: C11ORF4.hAug05
Intron
Length: 134
Transcript: C11ORF4.hAug05
Intron
Length: 421
Transcript: C11ORF4.hAug05
Exon
Length: 127
Transcript: C11ORF4.iAug05
Exon
Length: 372
Transcript: C11ORF4.iAug05
Exon
Length: 50
Transcript: C11ORF4.iAug05
Exon
Length: 129
Transcript: C11ORF4.iAug05
Exon
Length: 112
Transcript: C11ORF4.iAug05
Intron
Length: 1129
Transcript: C11ORF4.iAug05
Intron
Length: 83
Transcript: C11ORF4.iAug05
Intron
Length: 1325
Transcript: C11ORF4.iAug05
Intron
Length: 134
Transcript: C11ORF4.iAug05
Reaction
Expected peak: 231
Associated peak: 243,244,245,246,251,257
Repeated 51 time
Molarity: 5.81M
Forward: C11ORF4.F1
Reverse: C11ORF4.R14
Reaction
Expected peak: 395
Associated peak: 433,434,435,436,437,438,439,440
Repeated 51 time
Molarity: 18.76M
Forward: C11ORF4.F6
Reverse: C11ORF4.R6
Exon
Length: 361
Transcript: C11ORF4.jAug05
Exon
Length: 50
Transcript: C11ORF4.jAug05
Exon
Length: 37
Transcript: C11ORF4.jAug05
Exon
Length: 25
Transcript: C11ORF4.jAug05
Intron
Length: 83
Transcript: C11ORF4.jAug05
Intron
Length: 895
Transcript: C11ORF4.jAug05
Intron
Length: 118
Transcript: C11ORF4.jAug05
Reaction
Expected peak: 101
Associated peak: 105,106,107,108
Repeated 51 time
Molarity: 13.42M
Forward: C11ORF4.F1
Reverse: C11ORF4.R1
Exon
Length: 523
Transcript: C11ORF4.kAug05
Exon
Length: 225
Transcript: C11ORF4.kAug05
Exon
Length: 50
Transcript: C11ORF4.kAug05
Exon
Length: 16
Transcript: C11ORF4.kAug05
Intron
Length: 1276
Transcript: C11ORF4.kAug05
Intron
Length: 83
Transcript: C11ORF4.kAug05
Intron
Length: 706
Transcript: C11ORF4.kAug05
Reaction
Expected peak: 248
Associated peak: 260,261,262,263
Repeated 51 time
Molarity: 0.49M
Forward: C11ORF4.F6
Reverse: C11ORF4.R6
Reaction
Expected peak: 408
Associated peak: None
Repeated 51 time
Molarity: 0.00M
Forward: C11ORF4.F1
Reverse: C11ORF4.R1
Exon
Length: 643
Transcript: C11ORF4.shed1.aAug05
Exon
Length: 372
Transcript: C11ORF4.shed2.aAug05
Exon
Length: 929
Transcript: C11ORF4.shed3.aAug05
Exon
Length: 673
Transcript: C11ORF4.shed4.aAug05
C11ORF4.R1
CTGGCACACACTGGTCCC
C11ORF4.R6
CTCGGAAGTAGAAGGAGAAGAGG
C11ORF4.F3
ACTCCCTGTTCGTCATCTGC
C11ORF4.R15
ATTGAGGATGTGGCTGGTGC
C11ORF4.R2
GAAGAGGATGAGGCCAAATACC
C11ORF4.F13
ACTAGCATCTACCTGGAGGCC
C11ORF4.F2
TCTTTCTGCTGGTGAACGTG
C11ORF4.R5
AGGGTGGTGGGCAGTAGCTC
C11ORF4.F6
GGCACTGGAGATAAGGAAAGG
C11ORF4.F1
TGTTCAAGGCCAAGGTGAAG
C11ORF4.R14
TCAAAGAAGTAGGACCGAGAGG
C11ORF4.R3
TTGTTCCCCAGGTCATTCAC
Gene primer pair list : Primer pairs
Forward Reverse Occurences View
C11ORF4.F1 C11ORF4.R1 51 View
C11ORF4.F1 C11ORF4.R14 51 View
C11ORF4.F13 C11ORF4.R15 51 View
C11ORF4.F13 C11ORF4.R5 51 View
C11ORF4.F2 C11ORF4.R2 51 View
C11ORF4.F3 C11ORF4.R3 51 View
C11ORF4.F6 C11ORF4.R6 51 View

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