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Identification of alternative splicing markers for breast cancer
2. Breast Validation Screen
Transcript map
: BCAS1
Exon
Length: 162
Transcript: BCAS1.aAug05
Length: 162
Transcript: BCAS1.aAug05
Exon
Length: 77
Transcript: BCAS1.aAug05
Length: 77
Transcript: BCAS1.aAug05
Exon
Length: 70
Transcript: BCAS1.aAug05
Length: 70
Transcript: BCAS1.aAug05
Exon
Length: 581
Transcript: BCAS1.aAug05
Length: 581
Transcript: BCAS1.aAug05
Exon
Length: 159
Transcript: BCAS1.aAug05
Length: 159
Transcript: BCAS1.aAug05
Exon
Length: 45
Transcript: BCAS1.aAug05
Length: 45
Transcript: BCAS1.aAug05
Exon
Length: 213
Transcript: BCAS1.aAug05
Length: 213
Transcript: BCAS1.aAug05
Exon
Length: 42
Transcript: BCAS1.aAug05
Length: 42
Transcript: BCAS1.aAug05
Exon
Length: 168
Transcript: BCAS1.aAug05
Length: 168
Transcript: BCAS1.aAug05
Exon
Length: 66
Transcript: BCAS1.aAug05
Length: 66
Transcript: BCAS1.aAug05
Exon
Length: 264
Transcript: BCAS1.aAug05
Length: 264
Transcript: BCAS1.aAug05
Exon
Length: 1916
Transcript: BCAS1.aAug05
Length: 1916
Transcript: BCAS1.aAug05
Intron
Length: 11709
Transcript: BCAS1.aAug05
Length: 11709
Transcript: BCAS1.aAug05
Intron
Length: 492
Transcript: BCAS1.aAug05
Length: 492
Transcript: BCAS1.aAug05
Intron
Length: 29112
Transcript: BCAS1.aAug05
Length: 29112
Transcript: BCAS1.aAug05
Intron
Length: 32341
Transcript: BCAS1.aAug05
Length: 32341
Transcript: BCAS1.aAug05
Intron
Length: 835
Transcript: BCAS1.aAug05
Length: 835
Transcript: BCAS1.aAug05
Intron
Length: 9512
Transcript: BCAS1.aAug05
Length: 9512
Transcript: BCAS1.aAug05
Intron
Length: 9856
Transcript: BCAS1.aAug05
Length: 9856
Transcript: BCAS1.aAug05
Intron
Length: 8315
Transcript: BCAS1.aAug05
Length: 8315
Transcript: BCAS1.aAug05
Intron
Length: 9408
Transcript: BCAS1.aAug05
Length: 9408
Transcript: BCAS1.aAug05
Intron
Length: 3736
Transcript: BCAS1.aAug05
Length: 3736
Transcript: BCAS1.aAug05
Intron
Length: 8435
Transcript: BCAS1.aAug05
Length: 8435
Transcript: BCAS1.aAug05
Reaction
Expected peak: 251
Associated peak: 258,259,260,261,262
Repeated 51 time
Molarity: 16.86M
Forward: BCAS1.F8
Reverse: BCAS1.R3
Expected peak: 251
Associated peak: 258,259,260,261,262
Repeated 51 time
Molarity: 16.86M
Forward: BCAS1.F8
Reverse: BCAS1.R3
Reaction
Expected peak: 292
Associated peak: 286,287,288,289,290,291,292,293,295
Repeated 51 time
Molarity: 12.58M
Forward: BCAS1.F9
Reverse: BCAS1.R4
Expected peak: 292
Associated peak: 286,287,288,289,290,291,292,293,295
Repeated 51 time
Molarity: 12.58M
Forward: BCAS1.F9
Reverse: BCAS1.R4
Reaction
Expected peak: 400
Associated peak: 417,418,419,420,421,422,423,427
Repeated 51 time
Molarity: 4.67M
Forward: BCAS1.F4
Reverse: BCAS1.R4
Expected peak: 400
Associated peak: 417,418,419,420,421,422,423,427
Repeated 51 time
Molarity: 4.67M
Forward: BCAS1.F4
Reverse: BCAS1.R4
Reaction
Expected peak: 418
Associated peak: 439,440,441,442,443,444,445,446,447
Repeated 51 time
Molarity: 16.44M
Forward: BCAS1.F8
Reverse: BCAS1.R17
Expected peak: 418
Associated peak: 439,440,441,442,443,444,445,446,447
Repeated 51 time
Molarity: 16.44M
Forward: BCAS1.F8
Reverse: BCAS1.R17
Reaction
Expected peak: 486
Associated peak: 479,480,481,482,483,484,485,487
Repeated 51 time
Molarity: 11.81M
Forward: BCAS1.F2
Reverse: BCAS1.R1
Expected peak: 486
Associated peak: 479,480,481,482,483,484,485,487
Repeated 51 time
Molarity: 11.81M
Forward: BCAS1.F2
Reverse: BCAS1.R1
Reaction
Expected peak: 501
Associated peak: 467,489,491,492,493,494,495,496
Repeated 51 time
Molarity: 7.55M
Forward: BCAS1.F2
Reverse: BCAS1.R2
Expected peak: 501
Associated peak: 467,489,491,492,493,494,495,496
Repeated 51 time
Molarity: 7.55M
Forward: BCAS1.F2
Reverse: BCAS1.R2
Reaction
Expected peak: 519
Associated peak: 482,498,500,505,506,510,511,512,517
Repeated 51 time
Molarity: 9.73M
Forward: BCAS1.F1
Reverse: BCAS1.R1
Expected peak: 519
Associated peak: 482,498,500,505,506,510,511,512,517
Repeated 51 time
Molarity: 9.73M
Forward: BCAS1.F1
Reverse: BCAS1.R1
Exon
Length: 113
Transcript: BCAS1.bAug05
Length: 113
Transcript: BCAS1.bAug05
Exon
Length: 77
Transcript: BCAS1.bAug05
Length: 77
Transcript: BCAS1.bAug05
Exon
Length: 70
Transcript: BCAS1.bAug05
Length: 70
Transcript: BCAS1.bAug05
Exon
Length: 581
Transcript: BCAS1.bAug05
Length: 581
Transcript: BCAS1.bAug05
Exon
Length: 159
Transcript: BCAS1.bAug05
Length: 159
Transcript: BCAS1.bAug05
Exon
Length: 45
Transcript: BCAS1.bAug05
Length: 45
Transcript: BCAS1.bAug05
Exon
Length: 213
Transcript: BCAS1.bAug05
Length: 213
Transcript: BCAS1.bAug05
Exon
Length: 42
Transcript: BCAS1.bAug05
Length: 42
Transcript: BCAS1.bAug05
Exon
Length: 264
Transcript: BCAS1.bAug05
Length: 264
Transcript: BCAS1.bAug05
Exon
Length: 1019
Transcript: BCAS1.bAug05
Length: 1019
Transcript: BCAS1.bAug05
Intron
Length: 11709
Transcript: BCAS1.bAug05
Length: 11709
Transcript: BCAS1.bAug05
Intron
Length: 492
Transcript: BCAS1.bAug05
Length: 492
Transcript: BCAS1.bAug05
Intron
Length: 29112
Transcript: BCAS1.bAug05
Length: 29112
Transcript: BCAS1.bAug05
Intron
Length: 32341
Transcript: BCAS1.bAug05
Length: 32341
Transcript: BCAS1.bAug05
Intron
Length: 835
Transcript: BCAS1.bAug05
Length: 835
Transcript: BCAS1.bAug05
Intron
Length: 9512
Transcript: BCAS1.bAug05
Length: 9512
Transcript: BCAS1.bAug05
Intron
Length: 9856
Transcript: BCAS1.bAug05
Length: 9856
Transcript: BCAS1.bAug05
Intron
Length: 21693
Transcript: BCAS1.bAug05
Length: 21693
Transcript: BCAS1.bAug05
Intron
Length: 8435
Transcript: BCAS1.bAug05
Length: 8435
Transcript: BCAS1.bAug05
Reaction
Expected peak: 166
Associated peak: 179,180,181,182
Repeated 51 time
Molarity: 2.32M
Forward: BCAS1.F4
Reverse: BCAS1.R4
Expected peak: 166
Associated peak: 179,180,181,182
Repeated 51 time
Molarity: 2.32M
Forward: BCAS1.F4
Reverse: BCAS1.R4
Reaction
Expected peak: 252
Associated peak: 258,259,260,261,277,278,279,280
Repeated 51 time
Molarity: 3.95M
Forward: BCAS1.F2
Reverse: BCAS1.R1
Expected peak: 252
Associated peak: 258,259,260,261,277,278,279,280
Repeated 51 time
Molarity: 3.95M
Forward: BCAS1.F2
Reverse: BCAS1.R1
Reaction
Expected peak: 267
Associated peak: 272,273,274,275,290,291
Repeated 51 time
Molarity: 3.22M
Forward: BCAS1.F2
Reverse: BCAS1.R2
Expected peak: 267
Associated peak: 272,273,274,275,290,291
Repeated 51 time
Molarity: 3.22M
Forward: BCAS1.F2
Reverse: BCAS1.R2
Reaction
Expected peak: 285
Associated peak: 286,287,288,289,290,307,309
Repeated 51 time
Molarity: 3.29M
Forward: BCAS1.F1
Reverse: BCAS1.R1
Expected peak: 285
Associated peak: 286,287,288,289,290,307,309
Repeated 51 time
Molarity: 3.29M
Forward: BCAS1.F1
Reverse: BCAS1.R1
Exon
Length: 301
Transcript: BCAS1.cAug05
Length: 301
Transcript: BCAS1.cAug05
Exon
Length: 159
Transcript: BCAS1.cAug05
Length: 159
Transcript: BCAS1.cAug05
Exon
Length: 45
Transcript: BCAS1.cAug05
Length: 45
Transcript: BCAS1.cAug05
Exon
Length: 135
Transcript: BCAS1.cAug05
Length: 135
Transcript: BCAS1.cAug05
Exon
Length: 213
Transcript: BCAS1.cAug05
Length: 213
Transcript: BCAS1.cAug05
Exon
Length: 168
Transcript: BCAS1.cAug05
Length: 168
Transcript: BCAS1.cAug05
Exon
Length: 264
Transcript: BCAS1.cAug05
Length: 264
Transcript: BCAS1.cAug05
Exon
Length: 71
Transcript: BCAS1.cAug05
Length: 71
Transcript: BCAS1.cAug05
Exon
Length: 4749
Transcript: BCAS1.cAug05
Length: 4749
Transcript: BCAS1.cAug05
Intron
Length: 32341
Transcript: BCAS1.cAug05
Length: 32341
Transcript: BCAS1.cAug05
Intron
Length: 835
Transcript: BCAS1.cAug05
Length: 835
Transcript: BCAS1.cAug05
Intron
Length: 2365
Transcript: BCAS1.cAug05
Length: 2365
Transcript: BCAS1.cAug05
Intron
Length: 7012
Transcript: BCAS1.cAug05
Length: 7012
Transcript: BCAS1.cAug05
Intron
Length: 18213
Transcript: BCAS1.cAug05
Length: 18213
Transcript: BCAS1.cAug05
Intron
Length: 13210
Transcript: BCAS1.cAug05
Length: 13210
Transcript: BCAS1.cAug05
Intron
Length: 8435
Transcript: BCAS1.cAug05
Length: 8435
Transcript: BCAS1.cAug05
Intron
Length: 3401
Transcript: BCAS1.cAug05
Length: 3401
Transcript: BCAS1.cAug05
Reaction
Expected peak: 185
Associated peak: 197,198,199,214,215,216
Repeated 51 time
Molarity: 13.91M
Forward: BCAS1.F8
Reverse: BCAS1.R3
Expected peak: 185
Associated peak: 197,198,199,214,215,216
Repeated 51 time
Molarity: 13.91M
Forward: BCAS1.F8
Reverse: BCAS1.R3
Reaction
Expected peak: 226
Associated peak: 231,232,233,234,235,236,237
Repeated 51 time
Molarity: 2.36M
Forward: BCAS1.F9
Reverse: BCAS1.R4
Expected peak: 226
Associated peak: 231,232,233,234,235,236,237
Repeated 51 time
Molarity: 2.36M
Forward: BCAS1.F9
Reverse: BCAS1.R4
Reaction
Expected peak: 352
Associated peak: 371,372,373,374,375,376,377,378
Repeated 51 time
Molarity: 1.60M
Forward: BCAS1.F8
Reverse: BCAS1.R17
Expected peak: 352
Associated peak: 371,372,373,374,375,376,377,378
Repeated 51 time
Molarity: 1.60M
Forward: BCAS1.F8
Reverse: BCAS1.R17
Reaction
Expected peak: 378
Associated peak: 380,383,388,393,394,395,433,434,435,436,437,438,439
Repeated 51 time
Molarity: 1.62M
Forward: BCAS1.F2
Reverse: BCAS1.R1
Expected peak: 378
Associated peak: 380,383,388,393,394,395,433,434,435,436,437,438,439
Repeated 51 time
Molarity: 1.62M
Forward: BCAS1.F2
Reverse: BCAS1.R1
Reaction
Expected peak: 393
Associated peak: 408,409,410,411,412,413,414,425,430,445,447,448,449,450,451,452
Repeated 51 time
Molarity: 1.04M
Forward: BCAS1.F2
Reverse: BCAS1.R2
Expected peak: 393
Associated peak: 408,409,410,411,412,413,414,425,430,445,447,448,449,450,451,452
Repeated 51 time
Molarity: 1.04M
Forward: BCAS1.F2
Reverse: BCAS1.R2
Reaction
Expected peak: 411
Associated peak: 424,426,427,428,429,430,431,433,455,459,461,462,463,464,465,466,467
Repeated 51 time
Molarity: 1.28M
Forward: BCAS1.F1
Reverse: BCAS1.R1
Expected peak: 411
Associated peak: 424,426,427,428,429,430,431,433,455,459,461,462,463,464,465,466,467
Repeated 51 time
Molarity: 1.28M
Forward: BCAS1.F1
Reverse: BCAS1.R1
Exon
Length: 240
Transcript: BCAS1.dAug05
Length: 240
Transcript: BCAS1.dAug05
Exon
Length: 159
Transcript: BCAS1.dAug05
Length: 159
Transcript: BCAS1.dAug05
Exon
Length: 45
Transcript: BCAS1.dAug05
Length: 45
Transcript: BCAS1.dAug05
Exon
Length: 135
Transcript: BCAS1.dAug05
Length: 135
Transcript: BCAS1.dAug05
Exon
Length: 213
Transcript: BCAS1.dAug05
Length: 213
Transcript: BCAS1.dAug05
Exon
Length: 59
Transcript: BCAS1.dAug05
Length: 59
Transcript: BCAS1.dAug05
Intron
Length: 32341
Transcript: BCAS1.dAug05
Length: 32341
Transcript: BCAS1.dAug05
Intron
Length: 835
Transcript: BCAS1.dAug05
Length: 835
Transcript: BCAS1.dAug05
Intron
Length: 2365
Transcript: BCAS1.dAug05
Length: 2365
Transcript: BCAS1.dAug05
Intron
Length: 7012
Transcript: BCAS1.dAug05
Length: 7012
Transcript: BCAS1.dAug05
Intron
Length: 31591
Transcript: BCAS1.dAug05
Length: 31591
Transcript: BCAS1.dAug05
Reaction
Expected peak: 210
Associated peak: 219,220,221,222
Repeated 51 time
Molarity: 3.09M
Forward: BCAS1.F2
Reverse: BCAS1.R1
Expected peak: 210
Associated peak: 219,220,221,222
Repeated 51 time
Molarity: 3.09M
Forward: BCAS1.F2
Reverse: BCAS1.R1
Reaction
Expected peak: 225
Associated peak: 234,235,236,237
Repeated 51 time
Molarity: 2.84M
Forward: BCAS1.F2
Reverse: BCAS1.R2
Expected peak: 225
Associated peak: 234,235,236,237
Repeated 51 time
Molarity: 2.84M
Forward: BCAS1.F2
Reverse: BCAS1.R2
Reaction
Expected peak: 243
Associated peak: 250,251,252,253,254
Repeated 51 time
Molarity: 2.75M
Forward: BCAS1.F1
Reverse: BCAS1.R1
Expected peak: 243
Associated peak: 250,251,252,253,254
Repeated 51 time
Molarity: 2.75M
Forward: BCAS1.F1
Reverse: BCAS1.R1
Exon
Length: 38
Transcript: BCAS1.eAug05
Length: 38
Transcript: BCAS1.eAug05
Exon
Length: 45
Transcript: BCAS1.eAug05
Length: 45
Transcript: BCAS1.eAug05
Exon
Length: 213
Transcript: BCAS1.eAug05
Length: 213
Transcript: BCAS1.eAug05
Exon
Length: 42
Transcript: BCAS1.eAug05
Length: 42
Transcript: BCAS1.eAug05
Exon
Length: 66
Transcript: BCAS1.eAug05
Length: 66
Transcript: BCAS1.eAug05
Exon
Length: 264
Transcript: BCAS1.eAug05
Length: 264
Transcript: BCAS1.eAug05
Exon
Length: 148
Transcript: BCAS1.eAug05
Length: 148
Transcript: BCAS1.eAug05
Intron
Length: 835
Transcript: BCAS1.eAug05
Length: 835
Transcript: BCAS1.eAug05
Intron
Length: 9512
Transcript: BCAS1.eAug05
Length: 9512
Transcript: BCAS1.eAug05
Intron
Length: 9856
Transcript: BCAS1.eAug05
Length: 9856
Transcript: BCAS1.eAug05
Intron
Length: 17891
Transcript: BCAS1.eAug05
Length: 17891
Transcript: BCAS1.eAug05
Intron
Length: 3736
Transcript: BCAS1.eAug05
Length: 3736
Transcript: BCAS1.eAug05
Intron
Length: 8435
Transcript: BCAS1.eAug05
Length: 8435
Transcript: BCAS1.eAug05
Reaction
Expected peak: 232
Associated peak: 242,243,244,245
Repeated 51 time
Molarity: 0.96M
Forward: BCAS1.F4
Reverse: BCAS1.R4
Expected peak: 232
Associated peak: 242,243,244,245
Repeated 51 time
Molarity: 0.96M
Forward: BCAS1.F4
Reverse: BCAS1.R4
Reaction
Expected peak: 318
Associated peak: 315,316,317,318,319,320,321,322
Repeated 51 time
Molarity: 1.61M
Forward: BCAS1.F2
Reverse: BCAS1.R1
Expected peak: 318
Associated peak: 315,316,317,318,319,320,321,322
Repeated 51 time
Molarity: 1.61M
Forward: BCAS1.F2
Reverse: BCAS1.R1
Reaction
Expected peak: 333
Associated peak: 336,337,338,339,340,341,342,343,344
Repeated 51 time
Molarity: 1.09M
Forward: BCAS1.F2
Reverse: BCAS1.R2
Expected peak: 333
Associated peak: 336,337,338,339,340,341,342,343,344
Repeated 51 time
Molarity: 1.09M
Forward: BCAS1.F2
Reverse: BCAS1.R2
Reaction
Expected peak: 351
Associated peak: 359,360,361,362,363,364,365,366,367
Repeated 51 time
Molarity: 1.22M
Forward: BCAS1.F1
Reverse: BCAS1.R1
Expected peak: 351
Associated peak: 359,360,361,362,363,364,365,366,367
Repeated 51 time
Molarity: 1.22M
Forward: BCAS1.F1
Reverse: BCAS1.R1
Exon
Length: 146
Transcript: BCAS1.fAug05
Length: 146
Transcript: BCAS1.fAug05
Exon
Length: 66
Transcript: BCAS1.fAug05
Length: 66
Transcript: BCAS1.fAug05
Exon
Length: 264
Transcript: BCAS1.fAug05
Length: 264
Transcript: BCAS1.fAug05
Exon
Length: 71
Transcript: BCAS1.fAug05
Length: 71
Transcript: BCAS1.fAug05
Exon
Length: 208
Transcript: BCAS1.fAug05
Length: 208
Transcript: BCAS1.fAug05
Intron
Length: 9408
Transcript: BCAS1.fAug05
Length: 9408
Transcript: BCAS1.fAug05
Intron
Length: 3736
Transcript: BCAS1.fAug05
Length: 3736
Transcript: BCAS1.fAug05
Intron
Length: 8435
Transcript: BCAS1.fAug05
Length: 8435
Transcript: BCAS1.fAug05
Intron
Length: 3401
Transcript: BCAS1.fAug05
Length: 3401
Transcript: BCAS1.fAug05
Reaction
Expected peak: 251
Associated peak: 258,259,260,261,262
Repeated 51 time
Molarity: 16.86M
Forward: BCAS1.F8
Reverse: BCAS1.R3
Expected peak: 251
Associated peak: 258,259,260,261,262
Repeated 51 time
Molarity: 16.86M
Forward: BCAS1.F8
Reverse: BCAS1.R3
Reaction
Expected peak: 292
Associated peak: 286,287,288,289,290,291,292,293,295
Repeated 51 time
Molarity: 12.58M
Forward: BCAS1.F9
Reverse: BCAS1.R4
Expected peak: 292
Associated peak: 286,287,288,289,290,291,292,293,295
Repeated 51 time
Molarity: 12.58M
Forward: BCAS1.F9
Reverse: BCAS1.R4
Reaction
Expected peak: 418
Associated peak: 439,440,441,442,443,444,445,446,447
Repeated 51 time
Molarity: 16.44M
Forward: BCAS1.F8
Reverse: BCAS1.R17
Expected peak: 418
Associated peak: 439,440,441,442,443,444,445,446,447
Repeated 51 time
Molarity: 16.44M
Forward: BCAS1.F8
Reverse: BCAS1.R17
Exon
Length: 238
Transcript: BCAS1.gAug05
Length: 238
Transcript: BCAS1.gAug05
Exon
Length: 159
Transcript: BCAS1.gAug05
Length: 159
Transcript: BCAS1.gAug05
Exon
Length: 45
Transcript: BCAS1.gAug05
Length: 45
Transcript: BCAS1.gAug05
Exon
Length: 166
Transcript: BCAS1.gAug05
Length: 166
Transcript: BCAS1.gAug05
Exon
Length: 3943
Transcript: BCAS1.gAug05
Length: 3943
Transcript: BCAS1.gAug05
Exon
Length: 246
Transcript: BCAS1.gAug05
Length: 246
Transcript: BCAS1.gAug05
Intron
Length: 32341
Transcript: BCAS1.gAug05
Length: 32341
Transcript: BCAS1.gAug05
Intron
Length: 835
Transcript: BCAS1.gAug05
Length: 835
Transcript: BCAS1.gAug05
Intron
Length: 2334
Transcript: BCAS1.gAug05
Length: 2334
Transcript: BCAS1.gAug05
Intron
Length: 7012
Transcript: BCAS1.gAug05
Length: 7012
Transcript: BCAS1.gAug05
Intron
Length: 6126
Transcript: BCAS1.gAug05
Length: 6126
Transcript: BCAS1.gAug05
Exon
Length: 385
Transcript: BCAS1.hAug05
Length: 385
Transcript: BCAS1.hAug05
Exon
Length: 45
Transcript: BCAS1.hAug05
Length: 45
Transcript: BCAS1.hAug05
Intron
Length: 1382
Transcript: BCAS1.hAug05
Length: 1382
Transcript: BCAS1.hAug05
Exon
Length: 443
Transcript: BCAS1.shed1.aAug05
Length: 443
Transcript: BCAS1.shed1.aAug05
BCAS1.R4
TCTTCTGCTTGTTCATCTCGG
TCTTCTGCTTGTTCATCTCGG
BCAS1.F4
TCAGTTAAAGAGGACTCAGTCCC
TCAGTTAAAGAGGACTCAGTCCC
BCAS1.F1
AAGTCACCCACCACTTCAGC
AAGTCACCCACCACTTCAGC
BCAS1.R17
GATGGATACTGGGTCTGTTTGC
GATGGATACTGGGTCTGTTTGC
BCAS1.R1
CTCTGGCGGTGTGATAGTCTTT
CTCTGGCGGTGTGATAGTCTTT
BCAS1.F9
GACCTCAACGAAGGAGATGC
GACCTCAACGAAGGAGATGC
BCAS1.F8
ACCAAGAACCTCTCTGATGGC
ACCAAGAACCTCTCTGATGGC
BCAS1.F2
GACAAAGCCAACTTTACATCCC
GACAAAGCCAACTTTACATCCC
BCAS1.R2
TCCTGTTGGTTCAGGCTCTG
TCCTGTTGGTTCAGGCTCTG
BCAS1.R3
GTTCTTTGGCTTCCTGCTTG
GTTCTTTGGCTTCCTGCTTG