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Supplementary data
Tumor microenvironment associated modifications of alternative splicing
Tissu quality control molecular markers (related to Fig. 1B and Table S1)
LCM discovery and validation sample set
Reference Genes
: primer pair list
| Gene | Forward | Reverse | |
|---|---|---|---|
| VIEW | B2M | B2M_G_3_f | B2M_G_3_r |
| VIEW | GAPDH | GAPDH_G_f | GAPDH_G_r |
| VIEW | HMBS | HMBS_G_3_f | HMBS_G_3_r |
| VIEW | MRPL19 | MRPL19_G_3_f | MRPL19_G_3_r |
| VIEW | PSMC4 | PSMC4_G_1_f | PSMC4_G_1_r |
| VIEW | PUM1 | PUM1_G_2_f | PUM1_G_2_r |
| VIEW | SDHA | SDHA_G_f | SDHA_G_r |
| VIEW | YWHAZ | YWHAZ_G_f | YWHAZ_G_r |
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Page 1 of 1, records 1 to 8 of 8
QPCR Relative Expression Quantification
: NF pairwise rate map
QPCR Relative Expression Quantification
: NF Histogram
QPCR Relative Expression Quantification
: Reference Genes list
Reference Genes For Relative Expression Analysis
- PUM1_G_2_f PUM1_G_2_r
- GAPDH_G_f GAPDH_G_r
- YWHAZ_G_f YWHAZ_G_r
QPCR Relative Expression Quantification
: Reports
Comments on the experiments
- same sample as discovery screen LCM epithelial but bulk
- same sample as discovery screen LCM epithelial but bulk
- same sample as discovery screen LCM epithelial
- same sample as discovery screen LCM epithelial
- same sample as discovery screen LCM epithelial
- same sample as discovery screen LCM epithelial
- same sample as discovery screen LCM epithelial
- same sample as discovery screen LCM epithelial
- same sample as discovery screen LCM epithelial
- same sample as discovery screen LCM epithelial
- same sample as discovery screen LCM epithelial
- same sample as discovery screen LCM epithelial
- same sample as discovery screen LCM epithelial
- same sample as discovery screen LCM epithelial
QPCR Genorm
: M -- average pairwise expression ratio
| Primer Pair | 8 | 7 | 6 | 5 | 4 | 3 | 2 | 1 |
|---|---|---|---|---|---|---|---|---|
| SDHA_G_f SDHA_G_r | 2.771 | |||||||
| HMBS_G_3_f HMBS_G_3_r | 2.377 | 2.209 | ||||||
| MRPL19_G_3_f MRPL19_G_3_r | 1.765 | 1.595 | 1.479 | |||||
| PSMC4_G_1_f PSMC4_G_1_r | 1.584 | 1.394 | 1.210 | 1.108 | ||||
| B2M_G_3_f B2M_G_3_r | 1.587 | 1.385 | 1.214 | 1.084 | 0.986 | |||
| PUM1_G_2_f PUM1_G_2_r | 1.504 | 1.274 | 1.053 | 0.912 | 0.980 | 0.980 | ||
| YWHAZ_G_f YWHAZ_G_r | 1.357 | 1.172 | 0.983 | 0.921 | 0.809 | 0.809 | ||
| GAPDH_G_f GAPDH_G_r | 1.292 | 1.114 | 0.931 | 0.865 | 0.790 | 0.790 |
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Page 1 of 1, records 1 to 8 of 8
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