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Second attempt test 
BSP Analysis : Spliceosome redesign primer validation

Spliceosome redesign primer validation

Event PCR Hit Detection Primers Primer test BSP test E
Forward name Reverse name Forward seq Reverse seq Curve NTC CGE Sequence Conclusion CT diff NTC Conclusion
C19orf29 C19orf29.G2.F1 C19orf29.G2.R1 AGCAGCTTCGTGCCCACAT AGTTTGTACAGCTTCTGCCGC 31.65 BAD BAD NR BAD - - - 1.68 +/- 0.64
C19orf29 C19orf29.G1.F1 C19orf29.G1.R1 ACAACCCCTTCGGAGACAACAA TGGCTGATCCCCTTCTTCTCCA 26.95 BAD GOOD NR BAD - - - 1.71 +/- 0.65
CCDC49 CCDC49.G1.F1 CCDC49.G1.R1 AGATGGCAAATTCCTCCCCTGT ACCCTGGTTACGGTCAGAGTT 27.27 BAD GOOD NR BAD - - - 1.37 +/- 0.34
CCDC49 CCDC49.G2.F1 CCDC49.G2.R1 AAGAGGTCTACCAAAGGCGACA CCGTTTTCGCTCTAATTCCTCTGC 30.41 BAD GOOD NR BAD - - - 1.67 +/- 0.07
CCDC94 CCDC94.G1.F1 CCDC94.G1.R1 CGCTTTTACATCAAGTGCACGC TGCTCCATGGTGTAGTCTGTGT 27.42 BAD GOOD NR BAD - - - 1.32 +/- 0.28
CCDC94 CCDC94.G2.F1 CCDC94.G2.R1 AAGAGGGTGCAGAAGGAGCG TTGGAGTCCTTGGTCCGGTT 27.22 BAD GOOD NR BAD - - - 1.34 +/- 0.31
CDC5L CDC5L for1 CDC5L rev1 AACATTAATCCCGAGGATGG CAACCCTAAACGGAGATGTTC 25.71 BAD GOOD NR BAD - - - 1.34 +/- 0.31
CPSF1 CPSF1.G2.F1 CPSF1.G2.R1 AGGGAGAGCCTGGCTGAGGA CGATGATGTAGCTGGGCAGGAA 25.66 BAD GOOD NR BAD - - - 1.39 +/- 0.36
CPSF1 CPSF1.G1.F1 CPSF1.G1.R1 GCTCCTAAGCTTCAAGGATGCCA AGTGACAGGGTCTTCAGGTCAT 25.26 BAD GOOD NR BAD - - - 1.14 +/- 0.12
DHX9 DHX9.G1.F1 DHX9.G1.R1 CGATGTTGTTTATGTCATTGACTCCTGC GCCCATACGGTAGCATAGTTGGT 23.5 BAD GOOD NR BAD - - - 1.3 +/- 0.26
DHX9 DHX9.G2.F1 DHX9.G2.R1 CCTCAGCTGCTGGTATCAACCTT TATCGGGCCATCTTGGGAGGA 23.56 BAD GOOD NR BAD - - - 1.36 +/- 0.34
FLJ10292 MAGOHB.G2.F1 MAGOHB.G2.R1 AGCTTAGATATGCCAACAACAGCA TCAGTTCTTCCATTACACTCTTGTGC 30.07 BAD GOOD NR BAD - - - 1.54 +/- 0.14
FLJ10292 MAGOHB.G1.F1 MAGOHB.G1.R1 GCAAGTTTGGGCACGAGTTTCT GCTGTTGTTGGCATATCTAAGCTTTC 25.22 BAD GOOD NR BAD - - - 1.46 +/- 0.36
FUS FUS.G1.F1 FUS.G1.R1 CACCTTCAGCTAAAGCAGCTATTGAC GCAAATGAGACCTTGATAGGATTTCCG 34.65 BAD GOOD NR BAD - - - 1.35 +/- 0.26
FUS FUS.G2.F1 FUS.G2.R1 AAGGCCCCTAAACCAGATGGC ACGACGATCATCCCCGTAGTT 23.03 BAD GOOD NR BAD - - - 1.32 +/- 0.29
HNRPA1L3 HNRPA1L3.G1.F1 HNRPA1L3.G1.R1 ATGAGAGCCTGAGGAGCCATTT AGCGCTTGGTGTTTGGATCTCT 29.92 BAD GOOD NR BAD - - - 1.56 +/- 0.06
HNRPA1L3 HNRPA1L3.G2.F1 HNRPA1L3.G2.R1 AGTGGCAAGAAAAGGGGCTTTG GTTGTGGCCATTCACAGTATGGT 19.73 BAD GOOD NR BAD - - - 1.33 +/- 0.3
HNRPD HNRPD.G2.F1 HNRPD.G2.R1 GCACTATGTCGGAGGAGCAGTT ACCATGGCTCCCTCCTGCT 28.925 BAD GOOD NR BAD - - - 1.48 +/- 0.04
HNRPD HNRPD.G1.F1 HNRPD.G1.R1 ACCTTTAAGGAAGAAGAACCAGTGAAG TCCATGGGGAGCTCTATGGATTC 37.53 BAD BAD NR BAD - - - 1.27 +/- 0.23
HNRPLL HNRPLL.G2.F1 HNRPLL.G2.R1 TTTGCTGCAGATGAACCCGT TGGCCGAGTGATCCTTTTGCTT 24.63 BAD GOOD NR BAD - - - 1.31 +/- 0.26
HNRPLL HNRPLL.G1.F1 HNRPLL.G1.R1 CAGGAGGCAACAAAGTTCTTCTGC TGCACTTTGCCAACAGGGTT 24.79 BAD GOOD NR BAD - - - 1.08 +/- 0.06
IMP3 IMP3.G1.F1 IMP3.G1.R1 TTTGTGGAGCAAGGGCACGTA ACCCAAGTGACAAAGTCCTCCA 37.045 BAD GOOD NR BAD - - - 1.27 +/- 0.24
IMP3 IMP3.G2.F1 IMP3.G2.R1 ACAATGAGGAGCGCGATGACTT TCCAGCATCAGGCCTCAGTTTT 24.88 BAD GOOD NR BAD - - - 1.34 +/- 0.31
LOC100129585 LOC100129585.G1.F1 LOC100129585.G2.R1 ATCGCATGCCTATTCACCCAGT AGGTTCTTGCAGATTCCCAAGG 28.27 BAD GOOD NR BAD - - - 1.4 +/- 0.37
LOC100129585 LOC100129585.G1.F1 LOC100129585.G1.R1 ATCGCATGCCTATTCACCCAGT GGAGGTTCTTGCAGATTCCCAA 27.94 BAD GOOD NR BAD - - - 1.36 +/- 0.29
LSM7 LSM7.G2.F1 LSM7.G2.R1 AACCCCTTCATCCAGCAGCA AAGTCCGCGGGAAACCGAG 35.35 BAD GOOD NR BAD - - - 1.49 +/- 0.01
LSM7 LSM7.G1.F1 LSM7.G1.R1 ACTCCTCAACCTTGTGCTGGA TGTCCTCCGTGAGCTTGTACT 30.97 BAD GOOD NR BAD - - - 1.55 +/- 0.04
MBNL3 MBNL3 .G2.F1 MBNL3 .G2.R1 TGCAGCTTATGCTCCAAAACGC AGGATTGAAAGCCATGGGAGGA 25.42 BAD GOOD NR BAD - - - 1.3 +/- 0.28
MBNL3 MBNL3.G1.F1 MBNL3.G1.R1 TTTGCCGAGAATTTCAGCGTGG AAGCATCAGTAGGGTGAGCATAGC 25.16 BAD GOOD NR BAD - - - 1.32 +/- 0.29
MFAP1 MFAP1.G1.F1 MFAP1.G1.R1 CTACGGCGTTTACAGAACCGT TCTCCTACCACTTCAGGTTCCACT 25.69 BAD GOOD NR BAD - - - 1.27 +/- 0.26
MFAP1 MFAP1.G2.F1 MFAP1.G2.R1 AGGCATGGAAAGTTCGAGAGC TCGCATGCGTTCAATTTCTGC 26.1 BAD GOOD NR BAD - - - 1.32 +/- 0.28
POLR2E POLR2E.G2.F1 POLR2E.G2.R1 TGAAGATCATCCGGCCCAGT GGTAGCTACTGCACCAGCCG 24.75 BAD GOOD NR BAD - - - 1.31 +/- 0.28
POLR2E POLR2E.G1.F1 POLR2E.G1.R1 ATTTGTGCGAGATGCAGCCT CAGCGTGGTCTGGGTGAAGA 27.34 BAD GOOD NR BAD - - - 1.29 +/- 0.28
POLR2J POLR2J.G1.F1 POLR2J.G1.R1 AACGCCATCACCGACCTCAT TCTGGCCCCTACTCAATTCCTT 24.69 BAD GOOD NR BAD - - - 1.31 +/- 0.3
POLR2J POLR2J.G2.F1 POLR2J.G2.R1 ACACCGACGTGTCCTGTACATA ACCGCTGCTCAAGTCCACAT 27.86 BAD GOOD NR BAD - - - 1.32 +/- 0.27
POLR2K POLR2K.G2.F1 POLR2K.G2.R1 AGGGATCCAATCAGATGCAGAGA CCCAGCATTCATCGAGCATCAA 24.33 BAD GOOD NR BAD - - - 1.6 +/- 0.57
POLR2K POLR2K.G1.F1 POLR2K.G1.R1 TGATGCTCGATGAATGCTGGGA CAACAATCAGAAATGGGAAGAGAGCA 24.87 BAD GOOD NR BAD - - - 1.36 +/- 0.32
PPARGC1A PPARGC1A.G1.F1 PPARGC1A.G1.R1 CGCACCTTAAGTGTGGAACTCT TGGCTTTATGAGGAGGAGTGGT 37.855 BAD GOOD NR BAD - - - 1.39 +/- 0.24
PPARGC1A PPARGC1A.G2.F1 PPARGC1A.G2.R1 TCGCAGAGAGTATGAGAAGCGA CGACATAAATCACACGGCGCT 27.57 BAD GOOD NR BAD - - - 1.32 +/- 0.29
QKI QKI.G2.F1 QKI.G2.R1 ACGGAAAGACATGTACAATGACACA ACAGCATCAGGCAATTCTGCAC 24.28 BAD BAD NR BAD - - - 1.32 +/- 0.29
QKI QKI.G1.F1 QKI.G1.R1 ACTTCTGCGGGATCTTCAACCA TGTACATGTCTTTCCGTACTCTGC 24.55 BAD GOOD NR BAD - - - 1.35 +/- 0.3
RBM35B RBM35B.G2.F1 RBM35B.G2.R1 AATCGCTGGTTCGTGCCGAG TTGTCCAGCGGCTCTGCC BAD BAD BAD NR BAD - - - 1.18 +/- 0.17
RBM35B RBM35B_G_1_f RBM35B_G_2_r ATGGGCAGCAGCTATTGCGA AGAAGAACATGTCGGGGAGCA 26.82 BAD GOOD NR BAD - - - 1.31 +/- 0.29
RBM4B RBM4B.G1.F1 RBM4B.G1.R1 ATCAACGTGGAAGCCAGCAA TTGGTTGGTACAAGTGGGGCT 27.82 BAD GOOD NR BAD - - - 1.3 +/- 0.26
RBM4B RBM4B.G2.F1 RBM4B.G2.R1 GCAGTTCGAACACCTTACACCA TCGAGTGCTCCATATGCATCGT 26.95 BAD GOOD NR BAD - - - 1.37 +/- 0.35
RBM9 RBM9_prom_xs_f RBM9.G2.R1 GCATTCATGAAGAAGGAGGGAGATG CGTCTGTCTGTGCTCCACCTT BAD BAD BAD NR BAD - - - 1.56 +/- 0.02
RBM9 RBM9_prom_xs_f RBM9.G1.R1 GCATTCATGAAGAAGGAGGGAGATG TTGTGTCTGTGACTGCTGGC BAD BAD BAD NR BAD - - - 1.53 +/- 0.02
RBMY1A1 RBMY1A1.G3.F1 RBMY1A1.G3.R1 TGGTAGAGATCATTCTGAACATCTAAGTGGAAG TCGTGTATTACTATATGCGTGGCAGGT 36.08 BAD GOOD NR BAD - - - 1.35 +/- 0.32
RBMY1A1 RBMY1A1.G4.F1 RBMY1A1.G4.R1 GGCCCTTGGTAGAGATCATTCTGAACA GTGCTTCCACCATAAGACATCCGAG BAD BAD NR BAD - - - 1.03 +/- 0.03
RNUXA PHAX.G1.F1 PHAX.G1.R1 ACTCCTAGTATCAGCGAGGAACAAA TGTTCTCCTCTTCCTAGCAGCTT 30.31 BAD GOOD NR BAD - - - 1.35 +/- 0.01
RNUXA PHAX.G2.F1 PHAX.G2.R1 TCGATACGAGATCACAGCGGAA ACTCGGGCTATCAGGTCTTTCT 25.74 BAD GOOD NR BAD - - - 1.32 +/- 0.28
SCAF1 SCAF1.G1.F1 SCAF1.G1.R1 ATCCAGCAGGCTGTGGGAAG ATCGAAGGCCATGACACCGA 25.98 BAD GOOD NR BAD - - - 1.34 +/- 0.32
SCAF1 SCAF1.G2.F1 SCAF1.G2.R1 TGCCACGTCTGACAAGAGAGA TGCGTGTGCAGCTTCTTCAGAT 26.97 BAD GOOD NR BAD - - - 1.31 +/- 0.28
SCNM1 SCNM1.G1.F1 SCNM1.G1.R1 ACTATAACAGTTGCTGCCGCC TTTGACCTCTGAGGGTGGCATA 34.245 BAD BAD NR BAD - - - 1.31 +/- 0.19
SCNM1 SCNM1.G2.F1 SCNM1.G2.R1 AAAAGAAGAGTCGGGGACCTCCTA GGGCAGATGGCACAAGCAAA 26.23 BAD GOOD NR BAD - - - 1.33 +/- 0.3
SF1 SF1.G1.F1 SF1.G1.R1 TCAACTTTGTGGGGCTGCTCAT TCTTGGCATTGCACTCCTTCTC 23.55 BAD BAD NR BAD - - - 1.29 +/- 0.28
SF1 SF1.G2.F1 SF1.G2.R1 AGCGGCACAACCTCATCACA CACTCACACGTGTTGCTGGA 23.55 BAD GOOD NR BAD - - - 1.3 +/- 0.29
SF3A2 SF3A2.G2.F1 SF3A2.G2.R1 AGGTCAAGGTGGAGGTGAAGAA TCTCCGAGTCTCTCTGCTTGGT 23.9 BAD GOOD NR BAD - - - 1.31 +/- 0.28
SF3A2 SF3A2.G1.F1 SF3A2.G1.R1 TCGCCAAACGAAGTCGTGGA TGATGGCCTTAGGGCTTTAGCA 27.25 BAD GOOD NR BAD - - - 1.27 +/- 0.26
SF3B2 SF3B2.G1.F1 SF3B2.G1.R1 TGTTACTGGAGCAGGAACGACAG ACACCAATCTGGCCCATGTCTT 23.99 BAD GOOD NR BAD - - - 1.41 +/- 0.35
SF3B2 SF3B2.G2.F1 SF3B2.G2.R1 CCACTCCTACAGTTTTGCCCAT TCTCTGTTCTCATCTCCAGGGG 23.8 BAD BAD NR BAD - - - 1.4 +/- 0.38
SFRS2 SFRS2.G2.F1 SFRS2.G2.R1 TGTTGCAGTGCAGAGTGCTT TGTTTCTGCACGACAAGGCA 34.055 BAD GOOD NR BAD - - - 1.04 +/- 0.01
SFRS2 SFRS2.G1.F1 SFRS2.G1.R1 TCGGCAAGCAGTGTAAACGGA AGCCAGTTGCTTGTTCCAAGG 22.72 BAD GOOD NR BAD - - - 1.34 +/- 0.32
SRRM1 SRRM1_G_1_f SRRM1.G2.R1 GCAGCTGAAATTTGCAGAATGCCT TCCAAGGCTTTATAACCTCCAAATTTACT 24.47 BAD GOOD NR BAD - - - 1.32 +/- 0.29
SRRM1 SRRM1.G1.F1 SRRM1.G1.R1 GAACCTTCAGTACAAGAGGCTACTTC ACCTATCACTGAAACCCAGAGAGC 45.65 BAD BAD NR BAD - - - 1.28 +/- 0.25
SRRM2 SRRM2.G2.F1 SRRM2.G2.R1 TCTCCCGAGAAACTTCCCCAGT AAGAGCTGGCATGCCGAGAAA 23.28 BAD GOOD NR BAD - - - 1.31 +/- 0.26
SRRM2 SRRM2.G1.F1 SRRM2.G1.R1 AAACAGCCTAGCAGCCCTTATG GGCTAGGTCGAGTTGCAGATTT 22.37 BAD GOOD NR BAD - - - 1.3 +/- 0.26
U2AF1L4 U2AF1L4.G1.F1 U2AF1L4.G1.R1 GCTCCCGGGCTCCATAATGTAAT ATGCCGAAGTGAAACACGCA 28.71 BAD GOOD NR BAD - - - 1.29 +/- 0.26
U2AF1L4 U2AF1L4.G2.F1 U2AF1L4.G2.R1 AGGTGTTCACAGAACTGCAGGA TTGACATAGACGTTGCCCACGA 28.14 BAD GOOD NR BAD - - - 1.33 +/- 0.27
UPF3B UPF3B.G1.F1 UPF3B.G1.R1 AGTACAGAATCAATAGGCAGCTCAGA AAAGCTGCATCGCTGGACGA 26.08 BAD BAD NR BAD - - - 1.32 +/- 0.29
UPF3B UPF3B.G2.F1 UPF3B.G2.R1 TGTTCAGGGATCGCTTTGATGG AGGTGCAAATTCTACTATAGCGGG 26.11 BAD GOOD NR BAD - - - 1.33 +/- 0.31
XAB2 XAB2.G1.F1 XAB2.G1.R1 AGGAGCACAAGTACTTCGAGGA TTGGGCCACTTGAACAGCGA 25.69 BAD GOOD NR BAD - - - 1.38 +/- 0.25
XAB2 XAB2.G2.F1 XAB2.G2.R1 TACATCCGCAGCGGCCATTT TCAAACACCTGTGTGAAGTCCC 13.91 BAD GOOD NR BAD - - - 1.52 +/- 0.51
YBX1 YBX1.G2.F1 YBX1.G2.R1 CCGGCTTACCATCTCTACCATCAT GGTCTTCAGCTCCAATCTTTTGTTCA 21.37 BAD GOOD NR BAD - - - 1.35 +/- 0.33
YBX1 YBX1.G1.F1 YBX1.G1.R1 GGCGGGGACAAGAAGGTCAT CCATATCCGTTCCTTACATTGAACCA 21.21 BAD GOOD NR BAD - - - 1.4 +/- 0.38
YTHDC1 YTHDC1.G2.F1 YTHDC1.G2.R1 TGTCAGATCTGGTTCAGGCACA GGAGATATGCCTCTAGCTCTCTTCCT 25.33 BAD GOOD NR BAD - - - 1.36 +/- 0.33
YTHDC1 YTHDC1.G1.F1 YTHDC1.G1.R1 ATAACATGGGACCACCACCACC TGGAGCATGGTGCTGATAGTAAGG 29.12 BAD GOOD NR BAD - - - 1.59 +/- 0.1
ZMAT2 ZMAT2.G1.F1 ZMAT2.G1.R1 TGTCATTACCAAGACAACCCCTCA AGTCCTTCACCACACAGTCACA 25.52 BAD GOOD NR BAD - - - 1.33 +/- 0.3
ZMAT2 ZMAT2.G2.F1 ZMAT2.G2.R1 TTCGCCGAAAGTGGGACAAA TTGACAGGCTGCACTGGTTTTC 24.94 BAD GOOD NR BAD - - - 1.33 +/- 0.31

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