Back to Rnomics Platform
Open Session
×
1 warning:
- Access restricted to public data.
Please sign in at Rnomics Platform to access your data.
Home
Supplementary data
Tumor microenvironment associated modifications of alternative splicing
Quantitative RT-PCR primer validation
Splicing factors
Second attempt test
BSP Analysis
: Spliceosome redesign primer validation
Spliceosome redesign primer validation
| Event | PCR Hit | Detection | Primers | Primer test | BSP test | E | |||||||||
| Forward name | Reverse name | Forward seq | Reverse seq | Curve | NTC | CGE | Sequence | Conclusion | CT diff | NTC | Conclusion | ||||
| C19orf29 | C19orf29.G2.F1 | C19orf29.G2.R1 | AGCAGCTTCGTGCCCACAT | AGTTTGTACAGCTTCTGCCGC | 31.65 | BAD | BAD | NR | BAD | - | - | - | 1.68 +/- 0.64 | ||
| C19orf29 | C19orf29.G1.F1 | C19orf29.G1.R1 | ACAACCCCTTCGGAGACAACAA | TGGCTGATCCCCTTCTTCTCCA | 26.95 | BAD | GOOD | NR | BAD | - | - | - | 1.71 +/- 0.65 | ||
| CCDC49 | CCDC49.G1.F1 | CCDC49.G1.R1 | AGATGGCAAATTCCTCCCCTGT | ACCCTGGTTACGGTCAGAGTT | 27.27 | BAD | GOOD | NR | BAD | - | - | - | 1.37 +/- 0.34 | ||
| CCDC49 | CCDC49.G2.F1 | CCDC49.G2.R1 | AAGAGGTCTACCAAAGGCGACA | CCGTTTTCGCTCTAATTCCTCTGC | 30.41 | BAD | GOOD | NR | BAD | - | - | - | 1.67 +/- 0.07 | ||
| CCDC94 | CCDC94.G1.F1 | CCDC94.G1.R1 | CGCTTTTACATCAAGTGCACGC | TGCTCCATGGTGTAGTCTGTGT | 27.42 | BAD | GOOD | NR | BAD | - | - | - | 1.32 +/- 0.28 | ||
| CCDC94 | CCDC94.G2.F1 | CCDC94.G2.R1 | AAGAGGGTGCAGAAGGAGCG | TTGGAGTCCTTGGTCCGGTT | 27.22 | BAD | GOOD | NR | BAD | - | - | - | 1.34 +/- 0.31 | ||
| CDC5L | CDC5L for1 | CDC5L rev1 | AACATTAATCCCGAGGATGG | CAACCCTAAACGGAGATGTTC | 25.71 | BAD | GOOD | NR | BAD | - | - | - | 1.34 +/- 0.31 | ||
| CPSF1 | CPSF1.G2.F1 | CPSF1.G2.R1 | AGGGAGAGCCTGGCTGAGGA | CGATGATGTAGCTGGGCAGGAA | 25.66 | BAD | GOOD | NR | BAD | - | - | - | 1.39 +/- 0.36 | ||
| CPSF1 | CPSF1.G1.F1 | CPSF1.G1.R1 | GCTCCTAAGCTTCAAGGATGCCA | AGTGACAGGGTCTTCAGGTCAT | 25.26 | BAD | GOOD | NR | BAD | - | - | - | 1.14 +/- 0.12 | ||
| DHX9 | DHX9.G1.F1 | DHX9.G1.R1 | CGATGTTGTTTATGTCATTGACTCCTGC | GCCCATACGGTAGCATAGTTGGT | 23.5 | BAD | GOOD | NR | BAD | - | - | - | 1.3 +/- 0.26 | ||
| DHX9 | DHX9.G2.F1 | DHX9.G2.R1 | CCTCAGCTGCTGGTATCAACCTT | TATCGGGCCATCTTGGGAGGA | 23.56 | BAD | GOOD | NR | BAD | - | - | - | 1.36 +/- 0.34 | ||
| FLJ10292 | MAGOHB.G2.F1 | MAGOHB.G2.R1 | AGCTTAGATATGCCAACAACAGCA | TCAGTTCTTCCATTACACTCTTGTGC | 30.07 | BAD | GOOD | NR | BAD | - | - | - | 1.54 +/- 0.14 | ||
| FLJ10292 | MAGOHB.G1.F1 | MAGOHB.G1.R1 | GCAAGTTTGGGCACGAGTTTCT | GCTGTTGTTGGCATATCTAAGCTTTC | 25.22 | BAD | GOOD | NR | BAD | - | - | - | 1.46 +/- 0.36 | ||
| FUS | FUS.G1.F1 | FUS.G1.R1 | CACCTTCAGCTAAAGCAGCTATTGAC | GCAAATGAGACCTTGATAGGATTTCCG | 34.65 | BAD | GOOD | NR | BAD | - | - | - | 1.35 +/- 0.26 | ||
| FUS | FUS.G2.F1 | FUS.G2.R1 | AAGGCCCCTAAACCAGATGGC | ACGACGATCATCCCCGTAGTT | 23.03 | BAD | GOOD | NR | BAD | - | - | - | 1.32 +/- 0.29 | ||
| HNRPA1L3 | HNRPA1L3.G1.F1 | HNRPA1L3.G1.R1 | ATGAGAGCCTGAGGAGCCATTT | AGCGCTTGGTGTTTGGATCTCT | 29.92 | BAD | GOOD | NR | BAD | - | - | - | 1.56 +/- 0.06 | ||
| HNRPA1L3 | HNRPA1L3.G2.F1 | HNRPA1L3.G2.R1 | AGTGGCAAGAAAAGGGGCTTTG | GTTGTGGCCATTCACAGTATGGT | 19.73 | BAD | GOOD | NR | BAD | - | - | - | 1.33 +/- 0.3 | ||
| HNRPD | HNRPD.G2.F1 | HNRPD.G2.R1 | GCACTATGTCGGAGGAGCAGTT | ACCATGGCTCCCTCCTGCT | 28.925 | BAD | GOOD | NR | BAD | - | - | - | 1.48 +/- 0.04 | ||
| HNRPD | HNRPD.G1.F1 | HNRPD.G1.R1 | ACCTTTAAGGAAGAAGAACCAGTGAAG | TCCATGGGGAGCTCTATGGATTC | 37.53 | BAD | BAD | NR | BAD | - | - | - | 1.27 +/- 0.23 | ||
| HNRPLL | HNRPLL.G2.F1 | HNRPLL.G2.R1 | TTTGCTGCAGATGAACCCGT | TGGCCGAGTGATCCTTTTGCTT | 24.63 | BAD | GOOD | NR | BAD | - | - | - | 1.31 +/- 0.26 | ||
| HNRPLL | HNRPLL.G1.F1 | HNRPLL.G1.R1 | CAGGAGGCAACAAAGTTCTTCTGC | TGCACTTTGCCAACAGGGTT | 24.79 | BAD | GOOD | NR | BAD | - | - | - | 1.08 +/- 0.06 | ||
| IMP3 | IMP3.G1.F1 | IMP3.G1.R1 | TTTGTGGAGCAAGGGCACGTA | ACCCAAGTGACAAAGTCCTCCA | 37.045 | BAD | GOOD | NR | BAD | - | - | - | 1.27 +/- 0.24 | ||
| IMP3 | IMP3.G2.F1 | IMP3.G2.R1 | ACAATGAGGAGCGCGATGACTT | TCCAGCATCAGGCCTCAGTTTT | 24.88 | BAD | GOOD | NR | BAD | - | - | - | 1.34 +/- 0.31 | ||
| LOC100129585 | LOC100129585.G1.F1 | LOC100129585.G2.R1 | ATCGCATGCCTATTCACCCAGT | AGGTTCTTGCAGATTCCCAAGG | 28.27 | BAD | GOOD | NR | BAD | - | - | - | 1.4 +/- 0.37 | ||
| LOC100129585 | LOC100129585.G1.F1 | LOC100129585.G1.R1 | ATCGCATGCCTATTCACCCAGT | GGAGGTTCTTGCAGATTCCCAA | 27.94 | BAD | GOOD | NR | BAD | - | - | - | 1.36 +/- 0.29 | ||
| LSM7 | LSM7.G2.F1 | LSM7.G2.R1 | AACCCCTTCATCCAGCAGCA | AAGTCCGCGGGAAACCGAG | 35.35 | BAD | GOOD | NR | BAD | - | - | - | 1.49 +/- 0.01 | ||
| LSM7 | LSM7.G1.F1 | LSM7.G1.R1 | ACTCCTCAACCTTGTGCTGGA | TGTCCTCCGTGAGCTTGTACT | 30.97 | BAD | GOOD | NR | BAD | - | - | - | 1.55 +/- 0.04 | ||
| MBNL3 | MBNL3 .G2.F1 | MBNL3 .G2.R1 | TGCAGCTTATGCTCCAAAACGC | AGGATTGAAAGCCATGGGAGGA | 25.42 | BAD | GOOD | NR | BAD | - | - | - | 1.3 +/- 0.28 | ||
| MBNL3 | MBNL3.G1.F1 | MBNL3.G1.R1 | TTTGCCGAGAATTTCAGCGTGG | AAGCATCAGTAGGGTGAGCATAGC | 25.16 | BAD | GOOD | NR | BAD | - | - | - | 1.32 +/- 0.29 | ||
| MFAP1 | MFAP1.G1.F1 | MFAP1.G1.R1 | CTACGGCGTTTACAGAACCGT | TCTCCTACCACTTCAGGTTCCACT | 25.69 | BAD | GOOD | NR | BAD | - | - | - | 1.27 +/- 0.26 | ||
| MFAP1 | MFAP1.G2.F1 | MFAP1.G2.R1 | AGGCATGGAAAGTTCGAGAGC | TCGCATGCGTTCAATTTCTGC | 26.1 | BAD | GOOD | NR | BAD | - | - | - | 1.32 +/- 0.28 | ||
| POLR2E | POLR2E.G2.F1 | POLR2E.G2.R1 | TGAAGATCATCCGGCCCAGT | GGTAGCTACTGCACCAGCCG | 24.75 | BAD | GOOD | NR | BAD | - | - | - | 1.31 +/- 0.28 | ||
| POLR2E | POLR2E.G1.F1 | POLR2E.G1.R1 | ATTTGTGCGAGATGCAGCCT | CAGCGTGGTCTGGGTGAAGA | 27.34 | BAD | GOOD | NR | BAD | - | - | - | 1.29 +/- 0.28 | ||
| POLR2J | POLR2J.G1.F1 | POLR2J.G1.R1 | AACGCCATCACCGACCTCAT | TCTGGCCCCTACTCAATTCCTT | 24.69 | BAD | GOOD | NR | BAD | - | - | - | 1.31 +/- 0.3 | ||
| POLR2J | POLR2J.G2.F1 | POLR2J.G2.R1 | ACACCGACGTGTCCTGTACATA | ACCGCTGCTCAAGTCCACAT | 27.86 | BAD | GOOD | NR | BAD | - | - | - | 1.32 +/- 0.27 | ||
| POLR2K | POLR2K.G2.F1 | POLR2K.G2.R1 | AGGGATCCAATCAGATGCAGAGA | CCCAGCATTCATCGAGCATCAA | 24.33 | BAD | GOOD | NR | BAD | - | - | - | 1.6 +/- 0.57 | ||
| POLR2K | POLR2K.G1.F1 | POLR2K.G1.R1 | TGATGCTCGATGAATGCTGGGA | CAACAATCAGAAATGGGAAGAGAGCA | 24.87 | BAD | GOOD | NR | BAD | - | - | - | 1.36 +/- 0.32 | ||
| PPARGC1A | PPARGC1A.G1.F1 | PPARGC1A.G1.R1 | CGCACCTTAAGTGTGGAACTCT | TGGCTTTATGAGGAGGAGTGGT | 37.855 | BAD | GOOD | NR | BAD | - | - | - | 1.39 +/- 0.24 | ||
| PPARGC1A | PPARGC1A.G2.F1 | PPARGC1A.G2.R1 | TCGCAGAGAGTATGAGAAGCGA | CGACATAAATCACACGGCGCT | 27.57 | BAD | GOOD | NR | BAD | - | - | - | 1.32 +/- 0.29 | ||
| QKI | QKI.G2.F1 | QKI.G2.R1 | ACGGAAAGACATGTACAATGACACA | ACAGCATCAGGCAATTCTGCAC | 24.28 | BAD | BAD | NR | BAD | - | - | - | 1.32 +/- 0.29 | ||
| QKI | QKI.G1.F1 | QKI.G1.R1 | ACTTCTGCGGGATCTTCAACCA | TGTACATGTCTTTCCGTACTCTGC | 24.55 | BAD | GOOD | NR | BAD | - | - | - | 1.35 +/- 0.3 | ||
| RBM35B | RBM35B.G2.F1 | RBM35B.G2.R1 | AATCGCTGGTTCGTGCCGAG | TTGTCCAGCGGCTCTGCC | BAD | BAD | BAD | NR | BAD | - | - | - | 1.18 +/- 0.17 | ||
| RBM35B | RBM35B_G_1_f | RBM35B_G_2_r | ATGGGCAGCAGCTATTGCGA | AGAAGAACATGTCGGGGAGCA | 26.82 | BAD | GOOD | NR | BAD | - | - | - | 1.31 +/- 0.29 | ||
| RBM4B | RBM4B.G1.F1 | RBM4B.G1.R1 | ATCAACGTGGAAGCCAGCAA | TTGGTTGGTACAAGTGGGGCT | 27.82 | BAD | GOOD | NR | BAD | - | - | - | 1.3 +/- 0.26 | ||
| RBM4B | RBM4B.G2.F1 | RBM4B.G2.R1 | GCAGTTCGAACACCTTACACCA | TCGAGTGCTCCATATGCATCGT | 26.95 | BAD | GOOD | NR | BAD | - | - | - | 1.37 +/- 0.35 | ||
| RBM9 | RBM9_prom_xs_f | RBM9.G2.R1 | GCATTCATGAAGAAGGAGGGAGATG | CGTCTGTCTGTGCTCCACCTT | BAD | BAD | BAD | NR | BAD | - | - | - | 1.56 +/- 0.02 | ||
| RBM9 | RBM9_prom_xs_f | RBM9.G1.R1 | GCATTCATGAAGAAGGAGGGAGATG | TTGTGTCTGTGACTGCTGGC | BAD | BAD | BAD | NR | BAD | - | - | - | 1.53 +/- 0.02 | ||
| RBMY1A1 | RBMY1A1.G3.F1 | RBMY1A1.G3.R1 | TGGTAGAGATCATTCTGAACATCTAAGTGGAAG | TCGTGTATTACTATATGCGTGGCAGGT | 36.08 | BAD | GOOD | NR | BAD | - | - | - | 1.35 +/- 0.32 | ||
| RBMY1A1 | RBMY1A1.G4.F1 | RBMY1A1.G4.R1 | GGCCCTTGGTAGAGATCATTCTGAACA | GTGCTTCCACCATAAGACATCCGAG | BAD | BAD | NR | BAD | - | - | - | 1.03 +/- 0.03 | |||
| RNUXA | PHAX.G1.F1 | PHAX.G1.R1 | ACTCCTAGTATCAGCGAGGAACAAA | TGTTCTCCTCTTCCTAGCAGCTT | 30.31 | BAD | GOOD | NR | BAD | - | - | - | 1.35 +/- 0.01 | ||
| RNUXA | PHAX.G2.F1 | PHAX.G2.R1 | TCGATACGAGATCACAGCGGAA | ACTCGGGCTATCAGGTCTTTCT | 25.74 | BAD | GOOD | NR | BAD | - | - | - | 1.32 +/- 0.28 | ||
| SCAF1 | SCAF1.G1.F1 | SCAF1.G1.R1 | ATCCAGCAGGCTGTGGGAAG | ATCGAAGGCCATGACACCGA | 25.98 | BAD | GOOD | NR | BAD | - | - | - | 1.34 +/- 0.32 | ||
| SCAF1 | SCAF1.G2.F1 | SCAF1.G2.R1 | TGCCACGTCTGACAAGAGAGA | TGCGTGTGCAGCTTCTTCAGAT | 26.97 | BAD | GOOD | NR | BAD | - | - | - | 1.31 +/- 0.28 | ||
| SCNM1 | SCNM1.G1.F1 | SCNM1.G1.R1 | ACTATAACAGTTGCTGCCGCC | TTTGACCTCTGAGGGTGGCATA | 34.245 | BAD | BAD | NR | BAD | - | - | - | 1.31 +/- 0.19 | ||
| SCNM1 | SCNM1.G2.F1 | SCNM1.G2.R1 | AAAAGAAGAGTCGGGGACCTCCTA | GGGCAGATGGCACAAGCAAA | 26.23 | BAD | GOOD | NR | BAD | - | - | - | 1.33 +/- 0.3 | ||
| SF1 | SF1.G1.F1 | SF1.G1.R1 | TCAACTTTGTGGGGCTGCTCAT | TCTTGGCATTGCACTCCTTCTC | 23.55 | BAD | BAD | NR | BAD | - | - | - | 1.29 +/- 0.28 | ||
| SF1 | SF1.G2.F1 | SF1.G2.R1 | AGCGGCACAACCTCATCACA | CACTCACACGTGTTGCTGGA | 23.55 | BAD | GOOD | NR | BAD | - | - | - | 1.3 +/- 0.29 | ||
| SF3A2 | SF3A2.G2.F1 | SF3A2.G2.R1 | AGGTCAAGGTGGAGGTGAAGAA | TCTCCGAGTCTCTCTGCTTGGT | 23.9 | BAD | GOOD | NR | BAD | - | - | - | 1.31 +/- 0.28 | ||
| SF3A2 | SF3A2.G1.F1 | SF3A2.G1.R1 | TCGCCAAACGAAGTCGTGGA | TGATGGCCTTAGGGCTTTAGCA | 27.25 | BAD | GOOD | NR | BAD | - | - | - | 1.27 +/- 0.26 | ||
| SF3B2 | SF3B2.G1.F1 | SF3B2.G1.R1 | TGTTACTGGAGCAGGAACGACAG | ACACCAATCTGGCCCATGTCTT | 23.99 | BAD | GOOD | NR | BAD | - | - | - | 1.41 +/- 0.35 | ||
| SF3B2 | SF3B2.G2.F1 | SF3B2.G2.R1 | CCACTCCTACAGTTTTGCCCAT | TCTCTGTTCTCATCTCCAGGGG | 23.8 | BAD | BAD | NR | BAD | - | - | - | 1.4 +/- 0.38 | ||
| SFRS2 | SFRS2.G2.F1 | SFRS2.G2.R1 | TGTTGCAGTGCAGAGTGCTT | TGTTTCTGCACGACAAGGCA | 34.055 | BAD | GOOD | NR | BAD | - | - | - | 1.04 +/- 0.01 | ||
| SFRS2 | SFRS2.G1.F1 | SFRS2.G1.R1 | TCGGCAAGCAGTGTAAACGGA | AGCCAGTTGCTTGTTCCAAGG | 22.72 | BAD | GOOD | NR | BAD | - | - | - | 1.34 +/- 0.32 | ||
| SRRM1 | SRRM1_G_1_f | SRRM1.G2.R1 | GCAGCTGAAATTTGCAGAATGCCT | TCCAAGGCTTTATAACCTCCAAATTTACT | 24.47 | BAD | GOOD | NR | BAD | - | - | - | 1.32 +/- 0.29 | ||
| SRRM1 | SRRM1.G1.F1 | SRRM1.G1.R1 | GAACCTTCAGTACAAGAGGCTACTTC | ACCTATCACTGAAACCCAGAGAGC | 45.65 | BAD | BAD | NR | BAD | - | - | - | 1.28 +/- 0.25 | ||
| SRRM2 | SRRM2.G2.F1 | SRRM2.G2.R1 | TCTCCCGAGAAACTTCCCCAGT | AAGAGCTGGCATGCCGAGAAA | 23.28 | BAD | GOOD | NR | BAD | - | - | - | 1.31 +/- 0.26 | ||
| SRRM2 | SRRM2.G1.F1 | SRRM2.G1.R1 | AAACAGCCTAGCAGCCCTTATG | GGCTAGGTCGAGTTGCAGATTT | 22.37 | BAD | GOOD | NR | BAD | - | - | - | 1.3 +/- 0.26 | ||
| U2AF1L4 | U2AF1L4.G1.F1 | U2AF1L4.G1.R1 | GCTCCCGGGCTCCATAATGTAAT | ATGCCGAAGTGAAACACGCA | 28.71 | BAD | GOOD | NR | BAD | - | - | - | 1.29 +/- 0.26 | ||
| U2AF1L4 | U2AF1L4.G2.F1 | U2AF1L4.G2.R1 | AGGTGTTCACAGAACTGCAGGA | TTGACATAGACGTTGCCCACGA | 28.14 | BAD | GOOD | NR | BAD | - | - | - | 1.33 +/- 0.27 | ||
| UPF3B | UPF3B.G1.F1 | UPF3B.G1.R1 | AGTACAGAATCAATAGGCAGCTCAGA | AAAGCTGCATCGCTGGACGA | 26.08 | BAD | BAD | NR | BAD | - | - | - | 1.32 +/- 0.29 | ||
| UPF3B | UPF3B.G2.F1 | UPF3B.G2.R1 | TGTTCAGGGATCGCTTTGATGG | AGGTGCAAATTCTACTATAGCGGG | 26.11 | BAD | GOOD | NR | BAD | - | - | - | 1.33 +/- 0.31 | ||
| XAB2 | XAB2.G1.F1 | XAB2.G1.R1 | AGGAGCACAAGTACTTCGAGGA | TTGGGCCACTTGAACAGCGA | 25.69 | BAD | GOOD | NR | BAD | - | - | - | 1.38 +/- 0.25 | ||
| XAB2 | XAB2.G2.F1 | XAB2.G2.R1 | TACATCCGCAGCGGCCATTT | TCAAACACCTGTGTGAAGTCCC | 13.91 | BAD | GOOD | NR | BAD | - | - | - | 1.52 +/- 0.51 | ||
| YBX1 | YBX1.G2.F1 | YBX1.G2.R1 | CCGGCTTACCATCTCTACCATCAT | GGTCTTCAGCTCCAATCTTTTGTTCA | 21.37 | BAD | GOOD | NR | BAD | - | - | - | 1.35 +/- 0.33 | ||
| YBX1 | YBX1.G1.F1 | YBX1.G1.R1 | GGCGGGGACAAGAAGGTCAT | CCATATCCGTTCCTTACATTGAACCA | 21.21 | BAD | GOOD | NR | BAD | - | - | - | 1.4 +/- 0.38 | ||
| YTHDC1 | YTHDC1.G2.F1 | YTHDC1.G2.R1 | TGTCAGATCTGGTTCAGGCACA | GGAGATATGCCTCTAGCTCTCTTCCT | 25.33 | BAD | GOOD | NR | BAD | - | - | - | 1.36 +/- 0.33 | ||
| YTHDC1 | YTHDC1.G1.F1 | YTHDC1.G1.R1 | ATAACATGGGACCACCACCACC | TGGAGCATGGTGCTGATAGTAAGG | 29.12 | BAD | GOOD | NR | BAD | - | - | - | 1.59 +/- 0.1 | ||
| ZMAT2 | ZMAT2.G1.F1 | ZMAT2.G1.R1 | TGTCATTACCAAGACAACCCCTCA | AGTCCTTCACCACACAGTCACA | 25.52 | BAD | GOOD | NR | BAD | - | - | - | 1.33 +/- 0.3 | ||
| ZMAT2 | ZMAT2.G2.F1 | ZMAT2.G2.R1 | TTCGCCGAAAGTGGGACAAA | TTGACAGGCTGCACTGGTTTTC | 24.94 | BAD | GOOD | NR | BAD | - | - | - | 1.33 +/- 0.31 | ||
Copyright ©2008,2017, all rights reserved. 