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Home Supplementary data Tumor microenvironment associated modifications of alternative splicing Quantitative RT-PCR primer validation Splicing factors Third attempt permalink
Third attempt ⚙
BSP Analysis : primer validation

primer validation

Event PCR Hit Detection Primers Primer test BSP test E
Forward name Reverse name Forward seq Reverse seq Curve NTC CGE Sequence Conclusion CT diff NTC Conclusion
ACTB ACTB_G_3_f ACTB_G_3_r CGTGGGCCGCCCTAGGCACCA TTGGCCTTAGGGTTCAGGGGGG 18.62 GOOD BAD NR BAD - - - 1.09 +/- 0
HMG20B HMG20B_G_2_f HMG20B_G_1_r AAGGGCAAGAAGCGGAAGAAGA TCGTTCAGGAAGCGCACGTA 23.85 GOOD GOOD NR GOOD - - - 1.87 +/- 0
HMG20B HMG20B_G_3_f HMG20B_G_2_r CCGAGAGAGAGAAGCAGCAGT TGGATCTTCTCCGTGCACATCT 23.45 BAD GOOD NR BAD - - - 1.58 +/- 0
HMGCS2 HMGCS2_G_f HMGCS2_G_r GTGGTTACCCTGAGCTTAGC CGGGATGTGCTTGGCATTGA GOOD BAD NR BAD - - - 1.18 +/- 0
HNRPD HNRPD_G_1_f HNRPD.G2.R1 ACTATGTCGGAGGAGCAGTTCG ACCATGGCTCCCTCCTGCT 23.07 BAD BAD NR BAD - - - 1.62 +/- 0
HNRPD HNRPD_G_2_f HNRPD_G_1_r GCGTCTGGAGGCACCGAA TCCTCGTTCTTACTGGCGTCAA 22.28 GOOD GOOD NR GOOD - - - 1.62 +/- 0
HSPA1B HSPA1B_G_2_f HSPA1B_G_2_r CTGATCGGCCGCAAGTTCG ACCTTGGGCTTGTCTCCGTCGTTG 22.54 GOOD BAD NR BAD - - - 1.74 +/- 0
HSPA1B HSPA1B_G_3_f HSPA1B_G_3_r GCCATCGCCTACGGCC GGACACGTCGAAGGTGCCC 24.26 GOOD BAD NR BAD - - - 1.5 +/- 0
HSPA2 HSPA2_G_3_f HSPA2_G_2_r TTCGAGGTGGCCGTTAGTTGA ACTGAAAGGCGAGCGACGTTA 28.03 GOOD GOOD NR GOOD - - - 1.79 +/- 0
HSPA2 HSPA2_G_4_f HSPA2_G_3_r TGAACCCCACCAACACCATCTT TGTGGCATCCTCGAATTTCCGT 27.76 GOOD GOOD NR GOOD - - - 1.8 +/- 0
HSPA2 HSPA2_G_2_f HSPA2_G_1_r ACTTCGACAACCGCATGGTGA TGGGCCCAATGTCCTTCTTGT 27.77 GOOD GOOD NR GOOD - - - 1.94 +/- 0
HSPA7 HSPA7_G_3_f HSPA7_G_2_r AGGCCCAGATTCATGACTTCGT TCAGCTCCTTGCCGTTGAAGAA 29.2 GOOD BAD NR BAD - - - 1.68 +/- 0
HSPA7 HSPA7_G_2_f HSPA7_G_1_r TGCAAGAGGAAAGCCTTAGGGA TTGTCTTGCACTTTGCGCCT 29.98 GOOD BAD NR BAD - - - 2.13 +/- 0
LOC100129585 LOC100129585.G1.F1 LOC100129585.G2.R1 ATCGCATGCCTATTCACCCAGT AGGTTCTTGCAGATTCCCAAGG 29.02 GOOD GOOD NR GOOD - - - 1.55 +/- 0
LSM7 LSM7_G_3_f LSM7_G_3_r CTCGGTTTCCCGCGGACTT AAAATGCTTCCGTTCCAGGAGG 26.05 BAD GOOD NR BAD - - - 1.55 +/- 0
LSM7 LSM7_G_2_f LSM7_G_2_r GCACGTCCGTGGTGCTAATCT TGCTGCTGGATGAAGGGGTT 27.47 GOOD GOOD NR GOOD - - - 1.48 +/- 0
RBM35B RBM35B_G_4_f RBM35B_G_1_r TCCCCGACATGTTCTTCTCCTT CATGGTGGCCACAGTGAGGT 26.16 GOOD GOOD NR GOOD - - - 1.59 +/- 0
RBM35B RBM35B_G_1_f RBM35B_G_2_r ATGGGCAGCAGCTATTGCGA AGAAGAACATGTCGGGGAGCA 26.83 GOOD GOOD NR GOOD - - - 1.61 +/- 0
RBM9 RBM9_G_4_f RBM9_G_5_r AAGCCCAGTAGTTGGAGCTGT TTGCCTAGGGACACATCTGCTT 24.4 GOOD GOOD NR GOOD - - - 1.72 +/- 0
RBM9 RBM9_G_3_f RBM9.G2.R1 GCAACTCACCCAGCACACAAAA CGTCTGTCTGTGCTCCACCTT 24.73 GOOD BAD NR BAD - - - 1.59 +/- 0
RBM9 RBM9_G_4_f RBM9_G_4_r AAGCCCAGTAGTTGGAGCTGT TGCATCATTGCCTAGGGACACA 24.3 BAD GOOD NR BAD - - - 1.73 +/- 0
RBMY1A1 RBMY1A1_G_1_f RBMY1A1_G_2_r GCACTTCAGAGATACGGGACCT GTGGCAGGTGCTTCCACCATAA BAD GOOD BAD NR BAD - - - 1 +/- 0
RBMY1E RBMY1E_G_1_f RBMY1E_G_2_r GCACTTCAGAGATACGGGACCG GTGGCAGGTGCTTCCACCATAG GOOD BAD NR BAD - - - 1.11 +/- 0
RNU2-1 RNU2-1_G_2_f RNU2-1_G_r TCCGAGGACAATATATTAAATGGATTTTTGGAGCAG TGCAATACCAGGTCGATGCGT 21.86 BAD BAD NR BAD - - - 1.65 +/- 0
RNU2-1 RNU2-1_G_3_f RNU2-1_G_1_r TCCGAGGACAATATATTAAATGGATTTTTGGAGCA ACTGCAATACCAGGTCGATGC 22.09 BAD BAD NR BAD - - - 2.31 +/- 0
RNU2-2 RNU2-2_G_2_f RNU2-1_G_1_r TCCGAGGACAATATATTAAATGGATTTTTGGAACT ACTGCAATACCAGGTCGATGC 28.14 GOOD BAD NR BAD - - - 1.75 +/- 0
RNU2-2 RNU2-2_G_1_f RNU2-1_G_r TCCGAGGACAATATATTAAATGGATTTTTGGAACAT TGCAATACCAGGTCGATGCGT 33.13 BAD BAD NR BAD - - - 1.59 +/- 0

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