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Supplementary data
Tumor microenvironment associated modifications of alternative splicing
Splicing factor knockdown
Validation
RBFOX2 RBMX THOC4 SF3A2
QPCR Primer Pair List
: primer pair list
| Gene | Forward | Reverse | |
|---|---|---|---|
| VIEW | CDC5L | CDC5L for1 | CDC5L rev1 |
| VIEW | DDX17 | DDX17_G_1_f | DDX17_G_r |
| VIEW | DDX39 | DDX39_G_1_f | DDX39_G_r |
| VIEW | PSMC4 | PSMC4_G_1_f | PSMC4_G_1_r |
| VIEW | RBM35A | RBM35A_G_3_f | RBM35A_G_4_r |
| VIEW | RBM35B | RBM35B_G_4_f | RBM35B_G_1_r |
| VIEW | RBM38 | RBM38_G_f | RBM38_G_r |
| VIEW | RBM9 | RBM9_G_3_f | RBM9.G2.R1 |
| VIEW | RBMX | RBMX_G_1_f | RBMX_G_1_r |
| VIEW | SDHA | SDHA_G_f | SDHA_G_r |
| VIEW | SF3A1 | SF3A1_G_4_f | SF3A1_G_3_r |
| VIEW | SF3A2 | SF3A2.G1.F1 | SF3A2.G1.R1 |
| VIEW | THOC4 | THOC4_G_1_f | THOC4_G_r |
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Page 1 of 1, records 1 to 13 of 13
QPCR Relative Expression Quantification (SKOV3ip1)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | RBM35A_G_1_s;SKOV3ip1 |
| 2 | RBM35A_G_s;SKOV3ip1 |
| 3 | RBM35B_G_1_s;SKOV3ip1 |
| 4 | RBM35B_G_s;SKOV3ip1 |
| 5 | SI_RBM9_global_1;SKOV3ip1 |
| 6 | SI_RBM9_global_2;SKOV3ip1 |
| 7 | SI_RBMX_1;SKOV3ip1 |
| 8 | SI_RBMX_2;SKOV3ip1 |
| 9 | SKOV3ip1;Lipofectamine 2000 |
| 10 | THOC4__G_1_s;SKOV3ip1 |
| 11 | THOC4__G_s;SKOV3ip1 |
| 12 | allstar2_s;SKOV3ip1 |
| 13 | siCDC5L_global_1;SKOV3ip1 |
| 14 | siCDC5L_global_2;SKOV3ip1 |
| 15 | siDDX39_global_1;SKOV3ip1 |
| 16 | siDDX39_global_2;SKOV3ip1 |
| 17 | siSF3A2 global_1;SKOV3ip1 |
| 18 | siSF3A2 global_2;SKOV3ip1 |
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Page 1 of 1, records 1 to 18 of 18
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 | S11 | p11 | S12 | p12 | S13 | p13 | S14 | p14 | S15 | p15 | S16 | p16 | S17 | p17 | S18 | p18 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CDC5L for1 CDC5L rev1 | 1.000 | 0.885 | 0.108 | 0.155 | ||||||||||||||||||||||||||||||||
| DDX39_G_1_f DDX39_G_r | 1.000 | 0.879 | 0.135 | 0.318 | ||||||||||||||||||||||||||||||||
| RBM35A_G_3_f RBM35A_G_4_r | 0.354 | 0.182 | 1.000 | 1.009 | ||||||||||||||||||||||||||||||||
| RBM35B_G_4_f RBM35B_G_1_r | 0.303 | 0.277 | 1.000 | 1.033 | ||||||||||||||||||||||||||||||||
| RBM9_G_3_f RBM9.G2.R1 | 0.204 | 0.148 | 1.000 | 1.122 | ||||||||||||||||||||||||||||||||
| RBMX_G_1_f RBMX_G_1_r | 0.144 | 0.182 | 1.000 | 1.067 | ||||||||||||||||||||||||||||||||
| SF3A2.G1.F1 SF3A2.G1.R1 | 1.000 | 1.077 | 0.095 | 0.082 | ||||||||||||||||||||||||||||||||
| THOC4_G_1_f THOC4_G_r | 1.000 | 0.087 | 0.122 | 1.092 |
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Page 1 of 1, records 1 to 8 of 8
QPCR Relative Expression Quantification (NIH:OVCAR3)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | NIH:OVCAR-3-Crashing;Lipofectamine 2000 |
| 2 | RBM38_Global_177_1_s;NIH:OVCAR-3-Crashing |
| 3 | RBM38_Global_1782_s;NIH:OVCAR-3-Crashing |
| 4 | SI_DDX17_1;NIH:OVCAR-3-Crashing |
| 5 | SI_DDX17_2;NIH:OVCAR-3-Crashing |
| 6 | allstar2_s;NIH:OVCAR-3-Crashing |
| 7 | siDDX39_global_1;NIH:OVCAR-3-Crashing |
| 8 | siDDX39_global_2;NIH:OVCAR-3-Crashing |
| 9 | siSF3A1 global_1;NIH:OVCAR-3-Crashing |
| 10 | siSF3A1 global_2;NIH:OVCAR-3-Crashing |
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Page 1 of 1, records 1 to 10 of 10
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| DDX17_G_1_f DDX17_G_r | 1.000 | 0.707 | 0.727 | 1.185 | ||||||||||||||||
| DDX39_G_1_f DDX39_G_r | 1.000 | 0.647 | 0.298 | 0.445 | ||||||||||||||||
| RBM38_G_f RBM38_G_r | 1.000 | 0.847 | 0.580 | 1.241 | ||||||||||||||||
| SF3A1_G_4_f SF3A1_G_3_r | 1.000 | 0.740 | 0.322 | 0.505 |
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Page 1 of 1, records 1 to 4 of 4
QPCR Reference Gene Page
: .
QPCR Reference Gene Page (SKOV3ip1)
: .
QPCR Reference Gene Page (NIH:OVCAR3)
: .
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